[Correlation between apoptosis and proliferation in gastric pre-carcinoam].

OBJECTIVE: To clarify the correlation between apoptosis/proliferation and the expressions of oncogene bcl-2 and P53 in the changes from superficial gastritis, intestinal metaplasia, dysplasia, gastric cancer in early stage to advanced gastric cancer.

METHODS: TUNEL method was used to detected apoptosis. Immunohistochemical analysis was used to detect the expressions of proliferating cellular nuclear antigen(PCNA), bcl-2 protein and P53 protein. Results In the patients of superficial gastritis, atrophic gastritis, intestinal metaplasia, dysplasia, early gastric cancer and advanced gastric cancer and advanced gastric cancer, apoptotic indexes were: (6.4 +/- 1.9)%, (14.1 +/- 4.1)%, (23.3 +/- 6.1)%, (17.4 +/- 2.6)%, (11.3 +/- 3.7)%, (6.3 +/- 2.0)% respectively. PCNA indexes were: (11.3 +/- 2.2)%, (18.9 +/- 6.5)%, (20.3 +/- 7.3)%, (40.0 +/- 10.6)%, (53.1 +/- 10.9)% and (72.4 +/- 18.4)% respectively; the expression rates of bcl-2 were 10.0%, 23.3%, 40.0%, 56.7%, 85.7% and 46.7%. The expression rates of P53 were: 0%, 0%, 0%, 4.3%, 14.3% and 53.3%. The difference in the majority of observed indexes were significant (P < 0.05-0.01).

CONCLUSION: During the changes of gastric cancer, the apoptotic and proliferating indexes incerased simultaneously in superficial gastritis, atrophic gastritis and intestinal metaplasia. However, PCNA indexes further increased but apoptotic indexes decreased in dysplasia, early gastric cancer and advanced gastric cancer and advanced gastric cancer. The former may be the phenomenon in early gastric cancer and advanced gastric cancer and advanced gastic cancer. The former may be the phenomenon in ear early gastric cancer the latter may be the phenomenon in advanced gastric cancer.