We have located links that may give you full text access.
Expression of cytokeratins and additional markers in undifferentiated lymph node metastases of the neck.
Anticancer Research 2000 November
OBJECTIVE: A variety of carcinomas (CA) can metastasize to the lymph nodes of the neck. Differentiation of CA in the lymph nodes according to their resemblance to the structure of origin is the basis of histopathological diagnosis. However, during the course of the disease, e.g. tumor recurrence after ablative surgery, these tissues can completely lose the ability to imitate typical structures of the organ that gave rise to malignant transformation. This can result in the inability of the pathologist to identify the origin of the metastases. The identification of the large group of cytokeratins (CK) as a member of the family of intermediate filaments has improved the diagnosis of epithelial tissues. An attempt was made to use CK antibodies to identify the organ of origin of poorly differentiated and anaplastic lymph node metastases of the neck.
METHODS: We investigated 34 routinely fixed (formalin/paraffin) lymph node metastases of the neck or specimens of these metastases. The tumors differed in terms of suspected primary tumor site and differentiation. Depending on the case history, diagnosis was performed by hematoxylin-eosin staining and by immunohistochemical staining of sections using antibodies against CK (CK nos. 1-10-11, 5-6, 6-8, 7, 8, 8-18, 13-15-16, 19 and 20) and against additional markers [vimentin, leukocyte common antigen (LCA), S-100, gross cystic disease fluid protein (GCDFP) and Epstein-Barr virus-induced latent membrane antigen (EBV-LMP)].
RESULTS: The histopathological diagnosis was lympho-epithelial CA (8 cases), thyroid gland CA (2 cases), mammary gland CA (5 cases), bronchial CA (4 cases), basaloid CA (3 cases), clear cell CA (2 cases), sebaceous CA (1 case) and pharyngeal CA (9 cases). Some metastases were anaplastic in differentiation (G3-4). The marker expression in the immunohistological sections supported the histopathological findings. In some cases diagnosis succeeded especially in evaluating the marker expression. For example, in lympho-epithelial CA the epithelial tumor cell formations were positive for CK 5-6 antibodies, while expression in the lymphatic cells was lacking. In mammary gland CA the tumor cells were clearly identified by their positivity for CK 7 and GCDFP antibodies, in contrast to the negative infiltrating cells surrounding the tumor cells.
CONCLUSIONS: In light of the case history details and the histopathological findings, immunohistological expression of various markers, especially the identification of CK subtypes, supported the differential diagnosis. In some cases the diagnosis may be established only by using CK profile and additional markers.
METHODS: We investigated 34 routinely fixed (formalin/paraffin) lymph node metastases of the neck or specimens of these metastases. The tumors differed in terms of suspected primary tumor site and differentiation. Depending on the case history, diagnosis was performed by hematoxylin-eosin staining and by immunohistochemical staining of sections using antibodies against CK (CK nos. 1-10-11, 5-6, 6-8, 7, 8, 8-18, 13-15-16, 19 and 20) and against additional markers [vimentin, leukocyte common antigen (LCA), S-100, gross cystic disease fluid protein (GCDFP) and Epstein-Barr virus-induced latent membrane antigen (EBV-LMP)].
RESULTS: The histopathological diagnosis was lympho-epithelial CA (8 cases), thyroid gland CA (2 cases), mammary gland CA (5 cases), bronchial CA (4 cases), basaloid CA (3 cases), clear cell CA (2 cases), sebaceous CA (1 case) and pharyngeal CA (9 cases). Some metastases were anaplastic in differentiation (G3-4). The marker expression in the immunohistological sections supported the histopathological findings. In some cases diagnosis succeeded especially in evaluating the marker expression. For example, in lympho-epithelial CA the epithelial tumor cell formations were positive for CK 5-6 antibodies, while expression in the lymphatic cells was lacking. In mammary gland CA the tumor cells were clearly identified by their positivity for CK 7 and GCDFP antibodies, in contrast to the negative infiltrating cells surrounding the tumor cells.
CONCLUSIONS: In light of the case history details and the histopathological findings, immunohistological expression of various markers, especially the identification of CK subtypes, supported the differential diagnosis. In some cases the diagnosis may be established only by using CK profile and additional markers.
Full text links
Related Resources
Trending Papers
Challenges in Septic Shock: From New Hemodynamics to Blood Purification Therapies.Journal of Personalized Medicine 2024 Februrary 4
Molecular Targets of Novel Therapeutics for Diabetic Kidney Disease: A New Era of Nephroprotection.International Journal of Molecular Sciences 2024 April 4
The 'Ten Commandments' for the 2023 European Society of Cardiology guidelines for the management of endocarditis.European Heart Journal 2024 April 18
A Guide to the Use of Vasopressors and Inotropes for Patients in Shock.Journal of Intensive Care Medicine 2024 April 14
Diagnosis and Management of Cardiac Sarcoidosis: A Scientific Statement From the American Heart Association.Circulation 2024 April 19
Essential thrombocythaemia: A contemporary approach with new drugs on the horizon.British Journal of Haematology 2024 April 9
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app
All material on this website is protected by copyright, Copyright © 1994-2024 by WebMD LLC.
This website also contains material copyrighted by 3rd parties.
By using this service, you agree to our terms of use and privacy policy.
Your Privacy Choices
You can now claim free CME credits for this literature searchClaim now
Get seemless 1-tap access through your institution/university
For the best experience, use the Read mobile app