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lncRNA and Osteosarcoma

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https://www.readbyqxmd.com/read/28902349/tug1-promotes-osteosarcoma-tumorigenesis-by-upregulating-ezh2-expression-via-mir-144-3p
#1
Jiaqing Cao, Xinyou Han, Xin Qi, Xiangyun Jin, Xiaolin Li
lncRNA-TUG1 (Taurine upregulated 1) is up-regulated and highly correlated with poor prognosis and disease status in osteosarcoma. TUG1 knockdown inhibits osteosarcoma cell proliferation, migration and invasion, and promotes apoptosis. However, its mechanism of action has not been well addressed. Growing evidence documented that lncRNA works as competing endogenous (ce)RNAs to modulate the expression and biological functions of miRNA. As a putative combining target of TUG1, miR-144-3p has been associated with the progress of osteosarcoma...
August 30, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28866868/the-lncrna-hnf1a-as1-is-a-negative-prognostic-factor-and-promotes-tumorigenesis-in-osteosarcoma
#2
Lijun Cai, Jinhan Lv, Yinquan Zhang, Junhong Li, Yinong Wang, Huilin Yang
Recent studies have revealed that long noncoding RNA HNF1A-antisense 1 (HNF1A-AS1) plays an important role in the development of several human malignancy entities. However, the expression and function of HNF1A-AS1 in the carcinogenesis and development of osteosarcoma remains unknown. In this study, we detected the HNF1A-AS1 levels in human osteosarcoma tissues and cell lines by quantitative real-time polymerase chain reaction (qRT-PCR), and investigated its role in osteosarcoma by using in vitro assays. Our study showed that HNF1A-AS1 expression was significantly up-regulated in human osteosarcoma tissues and cell lines compared with their normal counterparts, and its expression level was positively correlated with the distance metastasis (P = 0...
September 2, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28810936/lncrna-c2dat1-promotes-cell-proliferation-migration-and-invasion-by-targeting-mir-34a-5p-in-osteosarcoma-cells
#3
Daofu Jia, Yanping Niu, Dongling Li, Zhaorui Liu
Osteosarcoma is a highly aggressive malignant bone tumor with poor prognosis in cases. Evidence suggested that lncRNAsare deregulated in multiple cancers. In this study, we investigated the role of the lncRNA C2dat1 on the biological functions of osteosarcoma cells. The expressions of C2dat1, miR-34a-5p and Sirt1 in human osteosarcoma cells were altered by transfection with their specific vectors/shRNA, or mimic/inhibitor. Then, cell viability, migration, invasion and apoptosis were respectively assessed post-transfection...
August 15, 2017: Oncology Research
https://www.readbyqxmd.com/read/28750740/lncrna-odrul-contributes-to-osteosarcoma-progression-through-the-mir-3182-mmp2-axis
#4
Kun-Peng Zhu, Xiao-Long Ma, Chun-Lin Zhang
Recent findings have shown that lncRNA dysregulation is involved in many cancers, including osteosarcoma (OS). In a previous study, we reported a novel lncRNA, ODRUL, that could promote doxorubicin resistance in OS. We now report the function and underlying mechanism of ODRUL in regulating OS progression. We show that ODRUL is upregulated in OS tissues and cell lines and correlates with poor prognosis. ODRUL knockdown significantly inhibits OS cell proliferation, migration, invasion, and tumor growth in vitro and in vivo by decreasing matrix metalloproteinase (MMP) expression...
July 5, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28720111/lncrna-snhg15-contributes-to-proliferation-invasion-and-autophagy-in-osteosarcoma-cells-by-sponging-mir-141
#5
Ke Liu, Yi Hou, Yunke Liu, Jia Zheng
BACKGROUND: LncRNA small nucleolar RNA host gene 15 (SNHG15) was reported to play an oncogenic role in tumors. However, the role of SNHG15 and its molecular mechanism in osteosarcoma (OS) cells are largely unknown. METHODS: qRT-PCR was performed to evaluate the expression levels of SNHG15 and miR-141 in OS tissues and cells. Cell transfection with different siRNAs, miRNAs or pcDNAs into U2OS and MG63 cells were carried out by Lipofectamine 2000. The effects of SNHG15 and miR-141 on OS cell proliferation, invasion and the levels of autophagy-related proteins were analyzed by MTT assay, Transwell invasion/migration assay and western blot, respectively...
July 18, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28688193/knockdown-of-lncrna-hulc-inhibits-proliferation-migration-invasion-and-promotes-apoptosis-by-sponging-mir-122-in-osteosarcoma
#6
Daliang Kong, Yang Wang
Osteosarcoma is a rare malignant bone tumor with high degree of malignancy. HULC (highly upregulated in liver cancer), a long noncoding RNA (lncRNA) was involved in hepatocellular carcinoma development and progression, but its underlying mechanism in osteosarcoma is unknown. The aim of this study was to explore the functional role of HULC in osteosarcoma. The study was conducted in human osteosarcoma cell lines and the expression of HULC in the cell lines was detected by qRT-PCR. Furthermore, the effects of HULC on tumorigenicity of osteosarcoma cells were evaluated by in vitro assays...
July 8, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28679390/lncrna-ancr-regulates-the-cell-growth-of-osteosarcoma-by-interacting-with-ezh2-and-affecting-the-expression-of-p21-and-p27
#7
Fei Zhang, Hao Peng
BACKGROUND: Osteosarcoma (OS) is one of the most common malignant tumors developed in the bone. EZH2 has been found to play pivotal roles in the development of various cancers. LncRNA-ANCR (anti-differentiation ncRNA) has been reported to interact with EZH2 and regulated osteoblast differentiation. Our study aimed to investigate the effect of lncRNA-ANCR on the tumorigenesis of osteosarcoma and explore the underlying molecular mechanism. METHODS: RT-PCR was performed to detect the messenger RNA (mRNA) levels of lncRNA-ANCR, EZH2, p21, and p27 in OS tissues and cell lines...
July 5, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/28642170/lncrna-dancr-promotes-tumor-progression-and-cancer-stemness-features-in-osteosarcoma-by-upregulating-axl-via-mir-33a-5p-inhibition
#8
Nian Jiang, Xuedi Wang, Xianbiao Xie, Yan Liao, Ni Liu, Junfeng Liu, Nabo Miao, Jingnan Shen, Tingsheng Peng
lncRNAs regulate the initiation and progression of osteosarcoma, although the mechanism by which this occurs remains unknown. The present study shows that over-expression of the lncRNA DANCR increased osteosarcoma cell proliferation, migration, and invasion in vitro, as well as promoted xenograft tumor growth and lung metastasis in vivo. Mechanistically, DANCR promoted osteosarcoma progression by mediating cancer stem cells (CSCs) features. Moreover, pull-down assays and luciferase reporter assays indicated that DANCR upregulated expression of the receptor tyrosine kinase AXL by competitively binding to miR-33a-5p...
June 19, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28636101/knockdown-of-lncrna-meg3-inhibits-viability-migration-and-invasion-and-promotes-apoptosis-by-sponging-mir-127-in-osteosarcoma-cell
#9
Yang Wang, Daliang Kong
Osteosarcoma (OS) is one of the most common bone malignancies and occurs almost exclusively in children and adolescents. This study aimed to explore the role of lncRNA maternally expressed gene 3 (MEG3) in OS cells growth and metastasis, and to uncover the possible underlying mechanism. In this study, the expressions of MEG3 in five OS cell lines (MG63, OS-732, SaOS, G292, and 143B) and in a human osteoblast cell line hFOB1.19 were measured by qRT-PCR analysis. The expressions of MEG3, miR-127 and ZEB1 in OS-732 cells were overexpressed or suppressed by transfection...
June 21, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28602700/long-non-coding-rna-pvt1-promotes-glycolysis-and-tumor-progression-by-regulating-mir-497-hk2-axis-in-osteosarcoma
#10
Jingyi Song, Xiaolin Wu, Fengxia Liu, Maohua Li, Yuerong Sun, Yan Wang, Chao Wang, Kai Zhu, Xiujuan Jia, Bing Wang, Xuexiao Ma
Cancer cells usually utilize glucose as a carbon source for aerobic glycolysis, a phenomenon known as the Warburg effect. Increasing studies have shown that PVT1 (a long non-coding RNA, lncRNA) functions as critical regulator of multiple cancers. However, it remains totally unknown whether and how PVT1 regulates glucose metabolism in OS cells. In this study, we found that the expression of PVT1 were specifically increased in OS cells and tissues, and the upregulated PVT1 indicated poor prognosis. Glucose uptake, lactate production, and the expression of HK2 in OS cells were increased by overexpression of PVT1, while decreased by PVT1 knockdown...
August 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28590934/lncrna-fendrr-sensitizes-doxorubicin-resistance-of-osteosarcoma-cells-through-down-regulating-abcb1-and-abcc1
#11
Zhu Kun-Peng, Ma Xiao-Long, Zhang Chun-Lin
Long noncoding RNAs (LncRNAs) act as crucial regulators in various cancers including osteosarcoma (OS), yet their potential roles and molecular mechanisms in OS chemoresistance remain unclear. In the present study, we investigated the role and potential regulatory mechanism of the most down-regulated expressed lncRNA, FENDRR screened by our previous lncRNA microarray analysis between the paired doxorubicin-resistant and sensitive human osteosarcoma cell lines (MG63/DXR vs MG63). FENDRR expression was down-regulated in the doxorubicin-resistant OS cell lines and tissues and negatively correlated to the poor prognosis of OS patients...
May 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28560950/malat1-promotes-the-proliferation-and-metastasis-of-osteosarcoma-cells-by-activating-the-rac1-jnk-pathway-via-targeting-mir-509
#12
Yong Zhang, Qingfeng Dai, Fanying Zeng, Haichun Liu
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a long noncoding RNA (lncRNA) that contributes to the initiation and development of many solid tumors, including osteosarcoma (OS). In this study, we showed that MALAT1 was increased in human OS tissues and cell lines. MALAT1 knockdown suppressed OS cell growth and metastasis, induced OS cell apoptosis and delayed tumor growth in an OS xenograft model. We also detected downregulation of microRNA-509 (miR-509), a suppressor of OS growth, in OS tissues and cell lines...
May 26, 2017: Oncology Research
https://www.readbyqxmd.com/read/28558357/long-non-coding-rna-hoxa11-as-functions-as-a-competing-endogenous-rna-to-regulate-rock1-expression-by-sponging-mir-124-3p-in-osteosarcoma
#13
Mingxing Cui, Jingyu Wang, Qingjiang Li, Junlei Zhang, Jinling Jia, Xinli Zhan
Long non-coding RNAs (lncRNAs) have been strongly associated with various types of cancer. this study was to explore the critical role of lncRNA HOXA11-AS in osteosarcoma (OS) progression. Briefly, we should that the expression of HOXA11-AS was upregulated in OS tissues and cell lines. The high expression of HOXA11-AS was associated with advanced clinical stage, distant metastasis and poor overall survival of OS. In addition, We found that HOXA11-AS silencing suppressed OS cells proliferation, invasion and induced cell arrest in G0/G1 phase...
August 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28550663/-sensitivity-of-doxorubicin-resistant-osteosarcoma-cells-to-doxorubicin-regulated-by-long-non-coding-rna-nr_036444
#14
K P Zhu, C L Zhang
Objective: To investigate the mechanism of long non-coding RNA (LncRNA) NR_036444 mediated sensitivity of multidrug-resistant osteosarcoma to doxorubicin. Methods: LncRNA-mRNA combined microarray was used to screen the differential expressions of lncRNA and mRNA in doxorubicin-resistant MG63/DXR osteosarcoma cells and their paired doxorubicin-sensitive MG63 cells; qRT-PCR was used to check the consistency of microarray. LncRNA NR_036444 was over-expressed in MG63/DXR cells by lentrvirus. CCK-8 array was used to evaluate cell proliferation and the sensitivity of cells to doxorubicin; Flow cytometry was used to evaluate cell cycle and apoptosis...
April 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28519068/long-noncoding-rna-gas5-suppresses-cell-growth-and-epithelial-mesenchymal-transition-in-osteosarcoma-by-regulating-the-mir-221-arhi-pathway
#15
Kaishan Ye, Shuanke Wang, Haihong Zhang, Hua Han, Bing Ma, Wei Nan
Dysregulated long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) play key roles in the development of human cancers. The lncRNA growth arrest-specific 5 (GAS5) is reported to be a tumor suppressor in multiple cancers. However, the roles of GAS5 and its related miRNAs in osteosarcoma are poorly understood. This study explored the potential functions and mechanisms of GAS5 in the tumorigenesis of osteosarcoma. Here, the expression of GAS5, miR-221 and aplasia Ras homologue member I (ARHI) was determined in osteosarcoma tissues and cells by Real-time PCR (RT-qPCR)...
May 18, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28469961/long-non-coding-rna-ak093407-promotes-proliferation-and-inhibits-apoptosis-of-human-osteosarcoma-cells-via-stat3-activation
#16
Yongkun Wang, Tingting Liang, Yao Wang, Yan Huang, Ye Li
Osteosarcoma is a malignant tumor of the skeletal system. Long non-coding RNAs (lncRNAs) have been shown to play significant role in osteosarcoma. The present study evaluated the effects and mechanism of lncRNA AK093407 in osteosarcoma. The study included human osteosarcoma cell line, U-2OS. Cell proliferation, viability, and apoptosis were measured using Ki-67 proliferation assay, MTT assay, and Annexin V/PI staining assay, respectively. Relative mRNA and protein expressions were measured using qRT-PCR and western blot, respectively...
2017: American Journal of Cancer Research
https://www.readbyqxmd.com/read/28469952/long-noncoding-rna-atb-promotes-osteosarcoma-cell-proliferation-migration-and-invasion-by-suppressing-mir-200s
#17
Feng Han, Changhe Wang, Yi Wang, Liang Zhang
Long noncoding RNA activated by transforming growth factor-β (lncRNA-ATB) is a novel lncRNA, which is recently reported to have critical roles in carcinogenesis and progression of several cancers. However, the expression, clinical values, biological roles, and underlying molecular mechanisms of lncRNA-ATB in osteosarcoma are still known. In this study, we measured lncRNA-ATB expression in serum and osteosarcoma tissues of osteosarcoma patients, analyzed its diagnostic and prognostic values. Serum lncRNA-ATB is increased in osteosarcoma patients and could accurately discriminate osteosarcoma patients from healthy controls...
2017: American Journal of Cancer Research
https://www.readbyqxmd.com/read/28466821/long-non-codingrna-lncrna-tug1-and-the-prognosis-of-cancer-a-meta-analysis
#18
X-H Yu, W Guo, J Zhang, C Ma, A-J Chu, B-L Wen, X Zhang, X-Y Yan, C-M Wu, D-M Wang, Y-L Qu
Some studies assessed the association between lncRNA taurine-upregulated gene 1 (TUG1) and the survival in cancer. However, the results were inconclusive.  Therefore, we performed a meta-analysis to determine this association. We used the following electronic databases to search for eligible literature: PubMed, Embase, Chinese National Knowledge Infrastructure (CNKI) and Wanfang. We used ORs and 95% CIs to measure the association between TUG1 and the survival of cancer. There was no significant association between TUG1 and OS of cancer (HR=1...
March 31, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28415638/altered-long-non-coding-rnas-predict-worse-outcome-in-osteosarcoma-patients-evidence-from-a-meta-analysis
#19
Yanliang Yang, Shunli Wang, Teng Li
Long non-coding RNAs (lncRNAs) are emerging as promising prognostic biomarkers in an expanding list of malignant neoplasms. Here, we sought to investigate the strength of associations between lncRNA signatures and clinical outcomes in osteosarcoma. We conducted a systematic search of the online databases from inception to July 2016. Hazard ratios (HRs) with corresponding 95% confidence intervals (CIs) for the primary endpoints of overall survival (OS), progression-free survival (PFS) or event-free survival (EFS) were extracted and meta-analyzed...
May 23, 2017: Oncotarget
https://www.readbyqxmd.com/read/28415557/long-non-coding-rna-cta-sensitizes-osteosarcoma-cells-to-doxorubicin-through-inhibition-of-autophagy
#20
Zhengguang Wang, Zhendong Liu, Song Wu
Recently, several long non-coding RNAs (lncRNAs) have been implicated in osteosarcoma (OS). However, the regulatory roles of lncRNAs in chemotherapy resistance of OS still remain unclear. This study aimed to screen a novel lncRNA that contributes to chemotherapeutic resistance of OS, and to explore the underlying mechanisms. Our data showed that lncRNA CTA was markedly downregulated in OS tissues compared to their matched non-tumor tissues, and low expression of lncRNA CTA was significantly associated with the advanced clinical stage and tumor size...
May 9, 2017: Oncotarget
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