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Molecular-epidemiology

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https://www.readbyqxmd.com/read/28810241/molecular-epidemiology-of-charcot-marie-tooth-disease-in-northern-ostrobothnia-finland-a-population-based-study
#1
Maria Marttila, Laura Kytövuori, Seppo Helisalmi, Mika Kallio, Marjo Laitinen, Mikko Hiltunen, Mikko Kärppä, Kari Majamaa
BACKGROUND: Charcot-Marie-Tooth (CMT) disease is the most common hereditary neuromuscular disorder with a population prevalence of 9.7-82.3/100,000. In this study, we have estimated the prevalence of CMT and its subtypes in Finland and examined the frequency of molecular etiologies. METHODS: A population-based survey included adult patients with peripheral neuropathy from the province of Northern Ostrobothnia, Finland. Secondary causes of peripheral polyneuropathy were excluded and patients with clinical and neurophysiological features pertinent with CMT were included...
August 16, 2017: Neuroepidemiology
https://www.readbyqxmd.com/read/28807095/simultaneous-detection-of-13-key-bacterial-respiratory-pathogens-by-combination-of-multiplex-pcr-and-capillary-electrophoresis
#2
Lu Xi Jiang, Hong Yu Ren, Hai Jian Zhou, Si Hong Zhao, Bo Yan Hou, Jian Ping Yan, Tian Qin, Yu Chen
OBJECTIVE: Lower respiratory tract infections continue to pose a significant threat to human health. It is important to accurately and rapidly detect respiratory bacteria. To compensate for the limits of current respiratory bacteria detection methods, we developed a combination of multiplex polymerase chain reaction (PCR) and capillary electrophoresis (MPCE) assay to detect thirteen bacterial pathogens responsible for lower respiratory tract infections, including Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Mycoplasma pneumoniae, Legionella spp...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28806866/molecular-screening-and-single-nucleotide-polymorphism-typing-of-molluscum-contagiosum-virus-mcv-from-genital-specimens-between-2012-and-2015
#3
Sedigheh Taghinezhad-S, Amir Hossein Mohseni, Hossein Keyvani, Narges Ghobadi
Background: The present study is the first comprehensive report of the Molluscum contagiosum virus (MCV) in Iran based on the molecular technique for differentiation and typing of the MCV1 and MCV2. Methods: Patients were diagnosed as having tumor-like genital warts less than 5 mm in diameter, and HIV seronegative samples were chosen for this cross-sectional study. TaqMan real-time PCR was used to identify MCV following clinical examination. Typing of the MCV-positive specimens was performed in the SNP A27451G region of MC021L gene...
August 15, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28801381/fluoroquinolone-resistance-mechanisms-by-molecular-epidemiologic-study-of-ciprofloxacin-nonsusceptible-escherichia-coli-sequence-types-isolated-from-clinical-specimens-in-a-tertiary-care-university-hospital-in-korea-emergence-of-clone-st131-2006-2008
#4
Hyung-Seok Yang, Youngjin Kim, Sun Young Cho, You Sun Nam, Tae Sang Oh, Kyung-Sun Park, Hyun-Chul Kim, Hee Joo Lee
To investigate the sequence types (STs) and fluoroquinolone resistance related mutations among ciprofloxacin (CIP)-non-susceptible extended-spectrum β-lactamase (ESBL)-producing E. coli isolated from Korean patients from 2006-2008. The prevalence of fluoroquinolone resistance-determining region (QRDR) mutations in gyrA, gyrB, parC, and parE and plasmid-mediated quinolone resistance (PMQR) genes were also studied. Multilocus sequence typing (MLST) was performed to identify STs. The most common ST was ST131 (33/51, 64...
August 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/28796034/the-association-between-xpg-polymorphisms-and-cancer-susceptibility-evidence-from-observational-studies
#5
Cuihong Han, Xiaoyi Huang, Ruixi Hua, Shujie Song, Lihua Lyu, Na Ta, Jinhong Zhu, Peixi Zhang
BACKGROUND: Exposure to environmental carcinogens can cause damages to DNA. If not properly repaired, the DNA damages may increase the risk of carcinogenesis. Xeroderma pigmentosum group G (XPG) gene is an essential gene in the nucleotide excision repair (NER) pathway. The association between XPG polymorphisms and cancer susceptibility has been the focus of attention in the molecular epidemiology of cancer. However, the conclusions have been divergent. Therefore, we conducted a comprehensive meta-analysis to precisely evaluate the association of 3 frequently investigated XPG polymorphisms (rs751402, rs873601, and rs2296147) with cancer risk...
August 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28789913/molecular-epidemiology-of-klebsiella-pneumoniae-invasive-infections-over-a-decade-at-kilifi-county-hospital-in-kenya
#6
Sonal P Henson, Christine J Boinett, Matthew J Ellington, Ngure Kagia, Salim Mwarumba, Sammy Nyongesa, Neema Mturi, Samuel Kariuki, J Anthony G Scott, Nicholas R Thomson, Susan C Morpeth
Multidrug resistant (MDR) Klebsiella pneumoniae is a common cause of nosocomial infections worldwide. Recent years have seen an explosion of resistance to extended-spectrum β-lactamases (ESBLs) and emergence of carbapenem resistance. Here, we examine 198 invasive K. pneumoniae isolates collected from over a decade in Kilifi County Hospital (KCH) in Kenya. We observe a significant increase in MDR K. pneumoniae isolates, particularly to third generation cephalosporins conferred by ESBLs. Using whole-genome sequences, we describe the population structure and the distribution of antimicrobial resistance genes within it...
July 22, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28783427/molecular-epidemiology-of-carbapenem-resistant-acinetobacter-baumannii-in-a-tertiary-care-hospital-in-egypt-clonal-spread-of-blaoxa-23
#7
Afaf Mohamed Samy El Bannah, Nada Nabil Nawar, Reem Mostafa Mohamed Hassan, Sherifa Tarek Bassiouni Salem
Of great concern is the increased frequency of carbapenem-resistant Acinetobacter baumannii (CRAB) causing healthcare-associated infections. Different classes of β-lactamases are involved in this resistance through hydrolyzing carbapenems. Multilocus sequence typing (MLST) has been applied successfully for characterizing different varieties of bacterial pathogens epidemiologically. In the present study, we aimed to type and characterize the resistance profile of clinical isolates of CRAB causing healthcare-associated infections in patients admitted to Kasr Al-Aini hospital, using MLST, and compare with sequence types (STs) from other countries...
August 7, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28782998/evaluation-of-whole-genome-mapping-as-a-fast-and-automated-molecular-epidemiological-tool-for-the-study-of-cronobacter-spp-in-powdered-infant-formula-processing-facilities
#8
Yanjie Tang, Geertrui Rasschaert, Liping Yu, Claire Chilton, Leen Baert
Cronobacter has been identified as the causative agent of outbreaks or sporadic cases of meningitis, necrotizing enterocolitis, and septicemia associated with powdered infant formula. Food processing environments may provide a possible contamination route. The purpose of this study was to evaluate whole genome mapping (WGM) as a fast and automated molecular epidemiological method for characterizing Cronobacter spp. in the processing environment. This is the first study indicating the applicability of WGM to Cronobacter...
August 7, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28781769/unraveling-neisseria-meningitidis-pathogenesis-from-functional-genomics-to-experimental-models
#9
REVIEW
Marco Soriani
Neisseria meningitidis is a harmless commensal bacterium finely adapted to humans. Unfortunately, under "privileged" conditions, it adopts a "devious" lifestyle leading to uncontrolled behavior characterized by the unleashing of molecular weapons causing potentially lethal disease such as sepsis and acute meningitis. Indeed, despite the lack of a classic repertoire of virulence genes in N. meningitidis separating commensal from invasive strains, molecular epidemiology and functional genomics studies suggest that carriage and invasive strains belong to genetically distinct populations characterized by an exclusive pathogenic potential...
2017: F1000Research
https://www.readbyqxmd.com/read/28776101/molecular-epidemiology-of-multidrug-resistant-clinical-isolates-of-acinetobacter-baumannii-a%C3%A2-10-year-analysis-in-a%C3%A2-large-tertiary-care-university-hospital-in-central-europe-with-international-admissions
#10
Luigi Segagni Lusignani, Peter Starzengruber, Verena Dosch, Ojan Assadian, Elisabeth Presterl, Magda Diab-Elschahawi
BACKGROUND: Over the last 10 years, multidrug resistant Acinetobacter baumannii has been spreading worldwide as emerging microorganisms that negatively impact on the outcome of in-hospital patients. METHODS: Between 2007 and 2016, all isolates of patients of the Vienna General Hospital (VGH), tested positive for multidrug resistant Acinetobacter baumannii (MDR A. baumannii) strains, were investigated with respect to their genetic relationship. Patient medical histories were reviewed in order to collect discriminating factors related to MDR A...
August 3, 2017: Wiener Klinische Wochenschrift
https://www.readbyqxmd.com/read/28774406/natural-history-of-acute-and-chronic-hepatitis-b-the-role-of-hbv-genotypes-and-mutants
#11
REVIEW
Chih-Lin Lin, Jia-Horng Kao
Molecular epidemiologic studies reveal remarkable differences in the geographical distribution of hepatitis B virus (HBV) genotypes. The frequency of mutants among HBV genotypes also varies. The role of HBV genotypes/mutants in the pathogenesis of HBV infection and natural history of HBV infection has been extensively investigated. The distribution of HBV genotypes in acute hepatitis B patients reflects the predominant genotypes in a given geographic area. In chronic hepatitis B patients, genotype C and D have a higher frequency of basal core promoter A1762T/G1764A mutations than genotype A and B...
June 2017: Best Practice & Research. Clinical Gastroenterology
https://www.readbyqxmd.com/read/28771142/comparison-of-semi-automated-commercial-rep-pcr-fingerprinting-spoligotyping-12-locus-miru-vntr-typing-and-single-nucleotide-polymorphism-analysis-of-the-embb-gene-as-molecular-typing-tools-for-mycobacterium-bovis
#12
Federica Armas, Cristina Camperio, Luana Coltella, Serena Selvaggini, Maria Beatrice Boniotti, Maria Lodovica Pacciarini, Vincenzo Di Marco Lo Presti, Cinzia Marianelli
PURPOSE: Highly discriminatory genotyping strategies are essential in molecular epidemiological studies of tuberculosis. In this study we evaluated, for the first time, the efficacy of the repetitive sequence-based PCR (rep-PCR) DiversiLab Mycobacterium typing kit over spoligotyping, 12-locus mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing and embB single nucleotide polymorphism (SNP) analysis for Mycobacterium bovis typing. METHODOLOGY: A total of 49 M...
August 4, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28770243/changing-pattern-of-classical-swine-fever-virus-genogroup-from-classical-1-1-to-emerging-2-2-in-india
#13
Vinod Kumar Singh, Kaushal Kishor Rajak, Ravi Kumar, Sachin D Raut, Arpit Saxena, Dhanavellu Muthuchelvan, Raj Kumar Singh, Awadh Bihari Pandey
Classical swine fever (CSF) is one of the most important viral diseases of pigs with high economic impact. The causative agent, Classical swine fever virus (CSFV) is a member of genus Pestivirus in family Flaviviredae and is structurally and antigenically related to other members of the genus. The identification of virus strains and genotypes can conveniently be used to trace the origin and patterns of virus spread, which contribut substantially in control strategies. In the present study, we have partially sequenced and analysed the 5' untranslated region (UTR) and E2 regions of CSFV clinical samples (n = 24) from various parts of the country...
June 2017: Virusdisease
https://www.readbyqxmd.com/read/28766060/noroviruses-and-sapoviruses-associated-with-acute-gastroenteritis-in-pediatric-patients-in-thailand-increased-detection-of-recombinant-norovirus-gii-p16-gii-13-strains
#14
Pattara Khamrin, Kattareeya Kumthip, Kanittapon Supadej, Aksara Thongprachum, Shoko Okitsu, Satoshi Hayakawa, Hiroshi Ushijima, Niwat Maneekarn
Enteric caliciviruses, including noroviruses (NoVs) and sapoviruses (SaVs), are recognized as important etiologic agents of acute gastroenteritis (AGE) with considerable genetic diversity. In order to gain an overview of the molecular epidemiology of human NoVs and SaVs in children hospitalized with AGE in Chiang Mai, Thailand, a total of 889 fecal specimens were collected from 2012 to 2014 and screened for NoVs and SaVs. Out of 889 fecal specimens, 154 (17.3%) and 6 (0.7%) were positive for NoV GII isolates and SaV, respectively...
August 1, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28763075/-group-b-streptococcal-perinatal-infection-a-global-latin-american-and-mexican-overview
#15
Gerardo C Palacios-Saucedo, Talyha Itzel Hernández-Hernández, Lydia Guadalupe Rivera-Morales, Evangelina Briones-Lara, Amílcar Caballero-Trejo, José M Vázquez-Guillén, Gustavo I Amador-Patiño, Ricardo García-Cabello, Fortino Solórzano-Santos, Cristina Rodríguez-Padillacs
Group B streptococci (Streptococcus agalactiae) cause a number of infections in women during pregnancy and postpartum, such as urinary tract infection, chorioamnionitis and endometritis, consequently may affect the newborn. Group B streptococci is the most common cause of severe infections in newborns in developed countries. Studies on the epidemiology of group B streptococci infections in Latin America are still limited. This information is also unknown in Mexico, but studies carried out in the center of the country have found high rates of vaginal colonization in pregnant women and there are case series and case reports of newborns...
May 2017: Gaceta Médica de México
https://www.readbyqxmd.com/read/28761458/genotyping-drug-susceptibility-and-prevalence-survey-of-trichomonas-vaginalis-among-women-attending-gynecology-clinics-in-hamadan-western-iran-in-2014-2015
#16
Mohammad Matini, Hossein Rezaei, Mohammad Fallah, Amir Hossein Maghsood, Massoud Saidijam, Tayebeh Shamsi-Ehsan
BACKGROUND: In spite of sufficient knowledge about phenotypic variation of Trichomonas vaginalis, its genetic characteristics are poorly understood. We carried out a molecular epidemiology study in which in vitro metronidazole susceptibility of T. vaginalis isolates was considered. METHODS: This study was conducted on 862 women admitted to Gynecology Clinics in Hamadan, west of Iran, during 2014-2015. After recording the socio-demographic and clinical characteristics of participants, vaginal swab samples were taken and subjected to microscopic examination, culture, in vitro sensitivity testing and PCR-restriction fragment length polymorphism (RFLP) analysis...
January 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28760895/molecular-epidemiology-and-phylogenomics-of-staphylococcus-aureus-bacteremia-in-latin-america-a-prospective-cohort-multicenter-study-in-nine-countries
#17
Cesar A Arias, Jinnethe Reyes, Lina Paola Carvajal, Sandra Rincon, Lorena Diaz, Diana Panesso, Gabriel Ibarra, Rafael Rios, Jose M Munita, Mauro J Salles, Carlos Alvarez-Moreno, Jaime Labarca, Coralith Garcia, Carlos M Luna, Carlos Mejia-Villatoro, Jeannete Zurita, Manuel Guzman-Blanco, Eduardo Rodriguez-Noriega, Apurva Narechania, Laura J Rojas, Paul J Planet, George M Weinstock, Eduardo Gotuzzo, Carlos Seas
Staphylococcus aureus are important pathogens causing a spectrum of diseases ranging from mild skin and soft tissue infections to life-threatening conditions. Bloodstream infections are particularly important and the treatment approach is complicated by the presence of methicillin resistance among S. aureus isolates (MRSA). Emergence of new genetic lineages of MRSA has occurred in Latin America (LA) with the rise and dissemination of the community-associated USA300 Latin American Variant (USA300-LV). Here, we prospectively characterized bloodstream MRSA recovered from selected hospitals in 9 Latin American countries...
July 31, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28760145/molecular-epidemiology-of-blastocystosis-in-malaysia-does-seasonal-variation-play-an-important-role-in-determining-the-distribution-and-risk-factors-of-blastocystis-subtype-infections-in-the%C3%A2-aboriginal-community
#18
Samseh Abdullah Noradilah, Norhayati Moktar, Tengku Shahrul Anuar, Ii Li Lee, Fatmah Md Salleh, Siti Nor Azreen Abdul Manap, Noor Shazleen Husnie Mohd Mohtar, Syed Muhamad Azrul, Wan Omar Abdullah, Anisah Nordin, Syamsa Rizal Abdullah
BACKGROUND: Alternating wet and dry seasons may play an important role in the acquisition and distribution of Blastocystis subtype infection in the tropics. This cross-sectional study was therefore conducted to provide the prevalence of Blastocystis and to determine the potential risk factors associated with each subtype during the wet and dry seasons in the Aboriginal community, Pahang, Malaysia. METHODS: A total of 473 faecal samples were collected: 256 (54.1%) and 217 (45...
July 31, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28758347/prevalence-complete-genome-sequencing-and-phylogenetic-analysis-of-porcine-deltacoronavirus-in-south-korea-2014-2016
#19
G Jang, K-K Lee, S-H Kim, C Lee
Porcine deltacoronavirus (PDCoV) is a newly emerged enterotropic swine coronavirus that causes enteritis and diarrhoea in piglets. Here, a nested reverse transcription (RT)-PCR approach for the detection of PDCoV was developed to identify and characterize aetiologic agent(s) associated with diarrhoeal diseases in piglets in South Korea. A PCR-based method was applied to investigate the presence of PDCoV in 683 diarrhoeic samples collected from 449 commercial pig farms in South Korea from January 2014 to December 2016...
July 30, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28757141/molecular-epidemiology-of-a-novel-re-assorted-epidemic-strain-of-equine-influenza-virus-in-pakistan-in-2015-16
#20
Amjad Khan, Muhammad Hassan Mushtaq, Mansur Ud Din Ahmad, Jawad Nazir, Shahid Hussain Farooqi, Asghar Khan
BACKGROUND: A widespread epidemic of equine influenza (EI) occurred in nonvaccinated equine population across multiple districts in Khyber Pakhtunkhwa Province of Pakistan during 2015-2016. OBJECTIVES AND METHODS: An epidemiological surveillance study was conducted from Oct 2015 to April 2016 to investigate the outbreak. EI virus strains were isolated in embryonated eggs from suspected equines swab samples and were subjected to genome sequencing using M13 tagged segment specific primers...
July 27, 2017: Virus Research
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