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https://www.readbyqxmd.com/read/28087900/itraq-based-quantitative-proteomic-analysis-and-bioinformatics-study-of-proteins-in-pterygia
#1
Dandan Linghu, Lili Guo, Yinghua Zhao, Zhiming Liu, Mingwei Zhao, Lvzhen Huang, Xiaoxin Li
PURPOSE: To analysis proteins in the tissue of pterygia, and to investigate their potential roles in pterygia, using the comparative proteomic technique of Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) coupled with offline 2DLC-MS/MS, Western-bolt. METHOD: The tissues of pterygia and healthy conjunctiva were collected from 10 pterygia patients (6 females, 4 males; average age was 52 years old; average course of disease was 6 years) in our hospital from September, 2015 to March, 2016...
January 13, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28079986/establishment-of-an-in-vitro-3-d-model-for-cartilage-damage-in-rheumatoid-arthritis-ra
#2
Yvonne Peck, Li Ting Leom, Pei Fen Patricia Low, Dong-An Wang
Rheumatoid arthritis (RA) is a chronic inflammatory disease that leads to progressive joint destruction. To further understand the process of rheumatoid cartilage damage, an in vitro model consisting of an interactive tri-culture of synovial fibroblasts (SFs), LPS-stimulated macrophages and a primary chondrocyte-based tissue engineered construct was established. The tissue engineered construct has a similar composition to human cartilage, which are rich in collagen type II and proteoglycans. Data generated from this model revealed that healthy chondrocytes were activated in the presence of SFs and macrophages...
January 12, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28071894/noninvasive-monitoring-of-blood-glucose-with-raman-spectroscopy
#3
Rishikesh Pandey, Santosh Kumar Paidi, Tulio A Valdez, Chi Zhang, Nicolas Spegazzini, Ramachandra Rao Dasari, Ishan Barman
The successful development of a noninvasive blood glucose sensor that can operate reliably over sustained periods of time has been a much sought after but elusive goal in diabetes management. Since diabetes has no well-established cure, control of elevated glucose levels is critical for avoiding severe secondary health complications in multiple organs including the retina, kidney and vasculature. While fingerstick testing continues to be the mainstay of blood glucose detection, advances in electrochemical sensing-based minimally invasive approaches have opened the door for alternate methods that would considerably improve the quality of life for people with diabetes...
January 10, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28062990/ultrastructural-aspects-and-molecular-phylogeny-of-auerbachia-maamouni-n-sp-myxosporea-bivalvulida-from-the-gallbladder-of-gnathanodon-speciosus-forssk%C3%A3-l-actinopterygii-carangidae-in-the-red-sea
#4
Lamjed Mansour, Carlos Azevedo, Ângela Alves, Saleh Al-Quraishy, Abdel-Azeem S Abdel-Baki
A new myxosporean parasite, Auerbachia maamouni n. sp., infecting the gallbladder of the golden trevally Gnathanodon speciosus Forsskål, is described, based on morphology, ultrastructure, and small subunit (SSU) rRNA gene sequencing. Of 50 fish collected from the Red Sea in Jeddah, Saudi Arabia, five were found heavily infected with mature myxospores floating free in the bile. Mature spores are pyriform to club-shaped with smooth valves, and contain a single polar capsule with an S-shaped polar filament, arranged in 13-16 polar filament coils, oriented longitudinally, with an irregular distribution on the polar capsule matrix...
January 2017: Systematic Parasitology
https://www.readbyqxmd.com/read/28036072/maternal-exposure-to-domestic-hair-cosmetics-and-occupational-endocrine-disruptors-is-associated-with-a-higher-risk-of-hypospadias-in-the-offspring
#5
Elodie Haraux, Karine Braun, Philippe Buisson, Erwan Stéphan-Blanchard, Camille Devauchelle, Jannick Ricard, Bernard Boudailliez, Pierre Tourneux, Richard Gouron, Karen Chardon
Pregnant women are exposed to various chemical products at home and at work. Some of these products contain endocrine-disrupting chemicals (EDCs) such as cosmetics, pesticides, industrial chemicals, heavy metals, plastics or medications that could alter sexual differentiation and increase the risk of hypospadias. We evaluated maternal occupational and household exposures that could constitute risk factors for hypospadias. From 2011 to 2014, we enrolled 57 full-term newborns with hypospadias and three randomly selected controls per case (162 control newborns), matched for gestational age, from 11 maternity units in Picardy, France...
December 29, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28035380/prox1-promotes-human-glioblastoma-cell-proliferation-and-invasion-via-activation-of-the-nuclear-factor-%C3%AE%C2%BAb-signaling-pathway
#6
Xuchang Xu, Xuefeng Wan, Xinting Wei
Prospero homeobox protein 1 (PROX1) is highly expressed in high-grade malignant astrocytic gliomas. However, the role of PROX1 in the pathogenesis of glioblastoma multiforme (GBM) remains unclear. The present study overexpressed PROX1 in human GBM cell lines and examined its effects on cell growth, tumorigenesis, and invasiveness. In addition, the involvement of the nuclear factor‑κB (NF‑κB) signaling pathway in the action of PROX1 was examined. It was identified that overexpression of PROX1 significantly increased the proliferation and colony formation of glioblastoma cells, compared with empty vector‑transfected controls...
December 27, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28030951/wide-scope-screening-method-for-multiclass-veterinary-drug-residues-in-fish-shrimp-and-eel-using-liquid-chromatography-quadrupole-high-resolution-mass-spectrometry
#7
Sherri B Turnipseed, Joseph M Storey, Jack J Lohne, Wendy Cook Andersen, Robert Burger, Aaron S Johnson, Mark R Madson
A screening method for veterinary drug residues in fish, shrimp and eel using LC with a high resolution MS instrument has been developed and validated. The method was optimized for over 70 test compounds representing a variety of veterinary drug classes. Tissues were extracted by vortex mixing with acetonitrile acidified with 2% acetic acid and 0.2% p-toluene sulfonic acid. A centrifuged portion of the extract was passed through a novel solid phase extraction cartridge designed to remove interfering matrix components from tissue extracts...
December 28, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28028594/major-phytopathogens-and-strains-from-cocoa-theobroma-cacao-l-are-differentiated-by-maldi-ms-lipid-and-or-peptide-protein-profiles
#8
Fábio Neves Dos Santos, Alessandra Tata, Kátia Roberta Anacleto Belaz, Dilze Maria Argôlo Magalhães, Edna Dora Martins Newman Luz, Marcos Nogueira Eberlin
Phytopathogens are the main disease agents that promote attack of cocoa plantations in all tropical countries. The similarity of the symptoms caused by different phytopathogens makes the reliable identification of the diverse species a challenge. Correct identification is important in the monitoring and management of these pests. Here we show that matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) in combination with multivariate data analysis is able to rapidly and reliably differentiate cocoa phytopathogens, namely Moniliophthora perniciosa, Phytophthora palmivora, P...
December 27, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28018596/developing-shared-understandings-of-recovery-and-care-a-qualitative-study-of-women-with-eating-disorders-who-resist-therapeutic-care
#9
Connie Musolino, Megan Warin, Tracey Wade, Peter Gilchrist
BACKGROUND: This paper explores the differing perspectives of recovery and care of people with disordered eating. We consider the views of those who have not sought help for their disordered eating, or who have been given a diagnosis but have not engaged with health care services. Our aim is to demonstrate the importance of the cultural context of care and how this might shape people's perspectives of recovery and openness to receiving professional care. METHOD: This study utilised a mixed methods approach of ethnographic fieldwork and psychological evaluation with 28 women from Adelaide, South Australia...
2016: Journal of Eating Disorders
https://www.readbyqxmd.com/read/28011753/a-review-on-machine-learning-principles-for-multi-view-biological-data-integration
#10
Yifeng Li, Fang-Xiang Wu, Alioune Ngom
Driven by high-throughput sequencing techniques, modern genomic and clinical studies are in a strong need of integrative machine learning models for better use of vast volumes of heterogeneous information in the deep understanding of biological systems and the development of predictive models. How data from multiple sources (called multi-view data) are incorporated in a learning system is a key step for successful analysis. In this article, we provide a comprehensive review on omics and clinical data integration techniques, from a machine learning perspective, for various analyses such as prediction, clustering, dimension reduction and association...
December 22, 2016: Briefings in Bioinformatics
https://www.readbyqxmd.com/read/28010119/measuring-sexual-orientation-a-review-and-critique-of-u-s-data-collection-efforts-and-implications-for-health-policy
#11
Margaret Wolff, Brooke Wells, Christina Ventura-DiPersia, Audrey Renson, Christian Grov
The U.S. Department of Health and Human Services' (HHS) Healthy People 2020 goals sought to improve health outcomes among sexual minorities; HHS acknowledged that a dearth of sexual orientation items in federal and state health surveys obscured a broad understanding of sexual minority-related health disparities. The HHS 2011 data progression plan aimed to advance sexual orientation data collection efforts at the national level. Sexual orientation is a complex, multidimensional construct often composed of sexual identity, sexual attraction, and sexual behavior, thus posing challenges to its quantitative and practical measurement and analysis...
December 23, 2016: Journal of Sex Research
https://www.readbyqxmd.com/read/27992039/-evaluation-of-the-mobile-emergency-care-service-in-santa-catarina-state-brazil
#12
Angela Maria Blatt Ortiga, Josimari Telino de Lacerda, Sonia Natal, Maria Cristina Marino Calvo
This case study evaluated the Mobile Emergency Care Service (SAMU) in Santa Catarina State, Brazil, in 2013/2014. The theoretical log frame and evaluation matrix were validated by expert consensus workshops. Two dimensions were proposed: emergency care management and emergency care, analyzed with 22 indicators. Data collection used interviews, direct observation in the eight regional SAMU dispatches, and a questionnaire sent to the coordinators of the municipal SAMU. The analysis and value judgment according to separate dimensions, sub-dimensions, and indicators allowed identifying strengths and weaknesses amenable to intervention...
December 15, 2016: Cadernos de Saúde Pública
https://www.readbyqxmd.com/read/27984758/determination-of-cortisol-in-lake-sturgeon-acipenser-fulvescens-eggs-by-liquid-chromatography-tandem-mass-spectrometry
#13
Ugo Bussy, Lydia Wassink, Kim T Scribner, Weiming Li
Quantifying cortisol concentrations in fish eggs is important to understand the effects of environmental conditions on maternal physiological condition and on egg provisioning and quality. Data are particularly relevant to studies of the ecology of threatened species such as lake sturgeon (Aciperser fulvescens) as well as assessments of larval physical and behavioral phenotypes, fish health and caviar quality in sturgeon aquaculture. This study focuses on development of bioanalytical methods for high sensitivity and robust determination of cortisol in sturgeon eggs...
January 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27974875/profiling-of-exercise-induced-transcripts-in-the-peripheral-blood-cells-of-thoroughbred-horses
#14
Teruaki Tozaki, Mio Kikuchi, Hironaga Kakoi, Kei-Ichi Hirota, Kazutaka Mukai, Hiroko Aida, Seiji Nakamura, Shun-Ichi Nagata
Transcriptome analyses based on DNA microarray technology have been used to investigate gene expression profiles in horses. In this study, we aimed to identify exercise-induced changes in the expression profiles of genes in the peripheral blood of Thoroughbred horses using DNA microarray technology (15,429 genes on 43,603 probes). Blood samples from the jugular vein were collected from six horses before and 1 min, 4 hr, and 24 hr after all-out running on a treadmill. After the normalization of microarray data, a total of 26,830 probes were clustered into four groups and 11 subgroups showing similar expression changes based on k-mean clustering...
2016: Journal of Equine Science
https://www.readbyqxmd.com/read/27973400/perfluorinated-compounds-in-greenhouse-and-open-agricultural-producing-areas-of-three-provinces-of-china-levels-sources-and-risk-assessment
#15
Yanwei Zhang, Dongfei Tan, Yue Geng, Lu Wang, Yi Peng, Zeying He, Yaping Xu, Xiaowei Liu
Field investigations on perfluoroalkyl acid (PFAA) levels in various environmental matrixes were reported, but there is still a lack of PFAA level data for agricultural environments, especially agricultural producing areas, so we collected soil, irrigation water and agricultural product samples from agricultural producing areas in the provinces of Liaoning, Shandong and Sichuan in China. The background pollution from instruments was removed and C₄-C18 PFAAs were detected by LC-MS/MS. The concentrations of PFAAs in the top and deep layers of soil were compared, and the levels of PFAAs in different agricultural environments (greenhouses and open agriculture) were analyzed...
December 10, 2016: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/27965133/abdominal-wall-reconstruction-with-concomitant-ostomy-associated-hernia-repair-outcomes-and-propensity-score-analysis
#16
Alexander F Mericli, Patrick B Garvey, Salvatore Giordano, Jun Liu, Donald P Baumann, Charles E Butler
BACKGROUND: The optimal strategy for abdominal wall reconstruction in the presence of a stomal-site hernia is unclear. We hypothesized that the rate of ventral hernia recurrence in patients undergoing a combined ventral hernia repair and stomal-site herniorraphy would not differ clinically from the ventral hernia recurrence rate in patients undergoing an isolated ventral hernia repair. We also hypothesized that bridged ventral hernia repairs result in worse outcomes compared to reinforced repairs regardless of stomal hernia...
December 10, 2016: Journal of the American College of Surgeons
https://www.readbyqxmd.com/read/27943225/polymorphisms-in-the-matrix-metalloproteinase-9-promoters-and-susceptibility-to-glial-tumors-in-turkey
#17
Mahmut Özden, Salim Katar, Hakan Hanimoğlu, Mustafa Onur Ulu, Cihan Işler, Oğuz Baran, Veysel Antar, Cumhur Gökhan Ekmekci, Mehmet Yaşar Kaynar
AIM: Evidences suggest an association between MMP-9 functional gene polymorphisms with several tumors. The aim of this study was to investigate the possible role of single-nucleotide polymorphisms (SNP) at MMP-9 R279Q A/G, P574R G/C and R668Q G/A and R668Q (rs17577) genotypes with glial tumors in Turkey. MATERIAL AND METHODS: The present series consisted of tissue samples obtained from 100 cancer-free controls and 100 patients who had undergone glial tumor resection from 2007 to 2011 at the Cerrahpasa Medical Faculty of Istanbul University...
November 11, 2016: Turkish Neurosurgery
https://www.readbyqxmd.com/read/27935129/performance-comparison-of-esi-and-apci-in-untargeted-and-targeted-lc-ms-based-metabolomics-analysis-of-grapeberry-metabolites
#18
Mauro Commisso, Andrea Anesi, Silvia Dal Santo, Flavia Guzzo
RATIONALE: Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) are both used to generate ions for the analysis of metabolites by liquid chromatography/mass spectrometry (LC-MS). We compared the performance of these methods for the analysis of Corvina grapevine berry methanolic extracts, which are complex mixtures of diverse metabolites. METHODS: Corvina berries representing three ripening stages (veraison, early-ripening and full-ripening) were collected during two growing seasons, powdered and extracted with methanol...
December 9, 2016: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/27932041/source-apportionment-and-health-risk-assessment-of-heavy-metals-in-soil-for-a-township-in-jiangsu-province-china
#19
Yanxue Jiang, Sihong Chao, Jianwei Liu, Yue Yang, Yanjiao Chen, Aichen Zhang, Hongbin Cao
Human activities contribute greatly to heavy metal pollution in soils. Concentrations of 15 metal elements were detected in 105 soil samples collected from a typical rural-industrial town in southern Jiangsu, China. Among them, 7 heavy metals-lead, copper, zinc, arsenic, chromium, cadmium, and nickel-were considered in the health risk assessment for residents via soil inhalation, dermal contact, and/or direct/indirect ingestion. Their potential sources were quantitatively apportioned by positive matrix factorization using the data set of all metal elements, in combination with geostatistical analysis, land use investigation, and industrial composition analysis...
February 2017: Chemosphere
https://www.readbyqxmd.com/read/27916264/the-use-of-conifer-needles-as-biomonitor-candidates-for-the-study-of-temporal-air-pollution-variation-in-the-strasbourg-region
#20
Josephine Al-Alam, Ziad Fajloun, Asma Chbani, Maurice Millet
The continuous emission of polluting chemicals into the atmosphere requires the implementation of monitoring of ambient air quality. The use of vegetation for environmental monitoring can be considered as a simple monitoring technique by providing a cheap and accessible matrix. In this study, needles of two conifers (Pinus nigra and Cedrus atlantica), were used for the consecutive biomonitoring of multipollutants such as pesticides, polychlorinated biphenyls, and polyaromatic hydrocarbons (PAHs) in an urban area in Strasbourg (France)...
February 2017: Chemosphere
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