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https://www.readbyqxmd.com/read/28715559/interferon-%C3%AE-treatment-of-human-laryngotracheal-stenosis-derived-fibroblasts
#1
Kevin Motz, Idris Samad, Linda X Yin, Michael K Murphy, Madhavi Duvvuri, Dacheng Ding, Alexander T Hillel
Importance: Laryngotracheal stenosis (LTS) is a fibroproliferative disorder of the glottis, subglottis, and trachea. In models of fibrosis from other organ systems, the CD4+ T-cell response has been shown to regulate extracellular matrix deposition. Specifically, helper T cell 2 (TH2) promotes fibrosis, whereas TH1 and associated cytokines have been shown to be antifibrotic. However, this antifibrotic effect of the TH1 response has not been demonstrated in LTS. Objective: To determine whether the TH1 cytokine interferon-γ inhibits the function of LTS-derived fibroblasts in vitro...
July 13, 2017: JAMA Otolaryngology—Head & Neck Surgery
https://www.readbyqxmd.com/read/28703288/a-comparative-proteomic-analysis-of-blood-serum-for-developmental-stages-in-pigs
#2
K Srikanth, E Lee, A Kwon, J Shin, H Chung
This study aimed to differentiate genes at developmental stages of pigs from 0 to 150 days of age, to build up a protein database and to find candidate genetic markers for growth traits. The analysis of two-dimensional electrophoresis and matrix-assisted laser-desorption/ionization mass spectrometry separated 252 protein segments. After successfully blasting the peptide sequences, the analysis confirmed 37 differentially expressed proteins that increased from birth to 150 days of age (type A), whereas the type B proteins presented the inverse pattern...
July 12, 2017: Animal Genetics
https://www.readbyqxmd.com/read/28701310/deletion-of-%C3%AE-1-integrin-in-collecting-duct-principal-cells-leads-to-tubular-injury-and-renal-medullary-fibrosis
#3
Fahmy A Mamuya, Dongping Xie, Lei Lei, Ming Huang, Kenji Tsuji, Diane E Capen, Baoxue Yang, Ralph Weissleder, Teodor G Păunescu, Hua Jenny Lu
The renal collecting duct (CD) contains two major cell types, intercalated (ICs) and principal cells (PCs). A previous report showed that deletion of β-1 integrin in the entire renal CD causes defective CD morphogenesis resulting in kidney dysfunction. However, subsequent deletion of β-1 integrin specifically in ICs, and PCs respectively did not cause any morphological defects in the CDs. The discrepancy between these studies prompts us to reinvestigate the role of β-1 integrin in CD cells, specifically in the PCs...
July 12, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28697595/the-effect-of-a-receding-saline-lake-the-salton-sea-on-airborne-particulate-matter-composition
#4
Alexander L Frie, Justin H Dingle, Samantha C Ying, Roya Bahreini
The composition of ambient particulate matter (PM) and its sources were investigated at the Salton Sea, a shrinking saline lake in California, U.S.A. To investigate the influence of playa exposure on PM composition, PM samples were collected during two seasons and at two sites around the Salton Sea. To characterize source composition, soil samples were collected from local playa and desert surfaces. PM and soil samples were analyzed for 15 elements using mass spectrometry and X-ray diffraction. The contribution of sources to PM mass and composition was investigated using Al-referenced enrichment factors (EFs) and source factors resolved from positive matrix factorization (PMF)...
July 12, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28678310/the-expression-of-the-brm-and-mmp2-genes-in-thoracic-aortic-aneurysm-and-aortic-dissection
#5
Y-H Li, X-M Li, M-S Lu, M-F Lv, X Jin
OBJECTIVE: To study the expression, roles, and clinical significance of Brahma (BRM) and matrix metalloproteinase 2 (MMP2) in the thoracic aortic aneurysm and aortic dissection. PATIENTS ND METHODS: Arterial specimens from 20 cases of thoracic aortic dissection and 38 cases of thoracic aortic aneurysm, as well as normal tissue were collected, paraffin-embedded, sectioned, and stained with anti-BRM and MMP2 monoclonal antibodies. Sections were analyzed by immunofluorescence, and the distribution and expression of BRM and MMP2 in the aortic wall were determined...
June 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28675134/overview-of-the-infant-and-young-child-feeding-policy-environment-in-pakistan-federal-sindh-and-punjab-context
#6
Hana Mahmood, Yasmeen Suleman, Tabish Hazir, Durre Samin Akram, Shahadat Uddin, Michael J Dibley, Saleem Abassi, Amara Shakeel, Narjis Kazmi, Anne Marie Thow
BACKGROUND: Appropriate infant and young child feeding (IYCF) practices have been identified as important for appropriate child growth and development. (Ministry of Planning and Development, Ministry of National Health Services, Regulations and Coordination (2012)) Children in Pakistan still experience high rates of malnutrition, indicating a likely need for stronger IYCF policy. The purpose of this study was to identify major stakeholders who shape the IYCF policy environment and analyze which policies protect, promote and support IYCF practices, either directly or indirectly...
June 13, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28668767/dynamic-changes-of-plasma-metabolites-in-pigs-with-galn-induced-acute-liver-failure-using-gc-ms-and-uplc-ms
#7
Ermei Chen, Juan Lu, Deying Chen, Danhua Zhu, Yini Wang, Yimin Zhang, Ning Zhou, Jie Wang, Jianzhou Li, Lanjuan Li
Metabolomics facilitates investigation of the mechanisms of disease and screening for biomarkers. Here, a gas chromatography-mass spectrometry (GC-MS) and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS)-based metabolomics approach was employed to identify plasma biomarkers of acute liver failure (ALF) in pigs. Blood was collected from pigs at 12h intervals during ALF. Hepatic injury was quantified by determining liver function and histopathology. Based on a multivariate data matrix and pattern recognition, two upregulated metabolites, namely, amino acids and conjugated bile acids, and two downregulated metabolites, lysophosphatidylcholines (LPCs) and phosphatidylcholines (PCs), were identified...
June 29, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28664175/ct-images-and-radiotherapy-treatment-planning-of-patients-with-breast-cancer-a-dataset
#8
Mohammad Rezaei, Ahmad Mohammadbeigi, Karim Khoshgard, Abbas Haghparast
The data presented here were originally collected for the research project "CT-Scan processing and analysis in patient with breast cancer after radiotherapy". Also, it reported in our study "Prediction of Lung Tissue Damage by Evaluating Clinical and Dosimetric Parameters in Breast Cancer Patients" (Hasanabdali et al., 2016) [1]. This article describes and directly links to 52 subjects referred to Mahdieh Oncology and Radiotherapy Center from February to August 2015. Treatment planning was done for delivering 50 Gy dose to PTV in 25 fractions...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28651344/uterine-responses-to-early-pre-attachment-embryos-in-the-domestic-dog-and-comparisons-with-other-domestic-animal-species
#9
Felix R Graubner, Aykut Gram, Ewa Kautz, Stefan Bauersachs, Selim Aslan, Ali R Agaoglu, Alois Boos, Mariusz P Kowalewski
In the dog there is no luteolysis in the absence of pregnancy. Thus, this species lacks any anti-luteolytic endocrine signal as found in other species that modulate uterine function during the critical period of pregnancy establishment. Nevertheless, in the dog an embryo-maternal communication must occur in order to prevent rejection of embryos. Based on this hypothesis, we performed microarray analysis of canine uterine samples collected during pre-attachment phase (days 10-12) and in corresponding non-pregnant controls, in order to elucidate the embryo attachment signal...
June 23, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28627627/stromal-antigen-2-functions-as-a-tumor-suppressor-in-bladder-cancer-cells
#10
Han Wang, Jianhua Zhong, Chenglong Wu, Yang Liu, Jinghua Zhang, Xiaowen Zou, Yan Chen, Jinfeng Su, Gang Yang, Yuantang Zhong, Aifa Tang
Stromal antigen 2 (STAG2) is a subunit of the cohesion complex that plays an important role in the normal segregation of sister chromatids during mitosis or meiosis. However, the effect of STAG2 on the bladder cancer cell proliferation, migration, and invasion has not yet been fully clarified. In this study, we aimed to characterize STAG2 expression and functional significance in BC and adjacent normal tissue. Notably, STAG2 expression was markedly lower in BC cells and tumor tissues than their normal counterparts at the gene and protein levels...
June 12, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28624524/a-direct-comparison-of-porcine-strattice%C3%A2-and-bovine-surgimend%C3%A2-acellular-dermal-matrices-in-implant-based-immediate-breast-reconstruction
#11
Jessica F Ball, Yezen Sheena, Dina M Tarek Saleh, Parto Forouhi, Sarah L Benyon, Michael S Irwin, Charles M Malata
INTRODUCTION: Acellular dermal matrix (ADM) assisted implant-based breast reconstruction (IBBR) has grown in popularity over traditional submuscular techniques. Numerous human, bovine or porcine derived ADMs are available with the type used varying considerably worldwide. Yet, comparative evidence for the efficacy of different ADMs particularly xenogenic is limited. This study directly compares early outcomes of porcine (Strattice™) and bovine (Surgimend™) ADMs in IBBR. METHOD: Retrospective study of sequential experience of immediate IBBR using Strattice or Surgimend ADM...
May 19, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/28618245/multi-omics-integrative-investigation-of-fatty-acid-metabolism-in-obese-and-lean-subcutaneous-tissue
#12
Flavia Badoud, Dyanne Brewer, Armen Charchoglyan, Daniel J Cuthbertson, David M Mutch
White adipose tissue (WAT) plays a central role in whole-body energy homeostasis through storage and release of fatty acids. A deeper understanding of the complex and highly integrated pathways regulating WAT fatty acid metabolism, and how they are altered with obesity, is necessary for diagnostic and therapeutic innovations in nutritional disorders. In this multi-omics study, we investigated the influence of obesity on fatty acid metabolism in human subcutaneous adipose tissue (SAT) using an approach that integrated transcriptomic, peptidomic, and fatty acid analyses...
July 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/28606174/using-multiclass-classification-to-automate-the-identification-of-patient-safety-incident-reports-by-type-and-severity
#13
Ying Wang, Enrico Coiera, William Runciman, Farah Magrabi
BACKGROUND: Approximately 10% of admissions to acute-care hospitals are associated with an adverse event. Analysis of incident reports helps to understand how and why incidents occur and can inform policy and practice for safer care. Unfortunately our capacity to monitor and respond to incident reports in a timely manner is limited by the sheer volumes of data collected. In this study, we aim to evaluate the feasibility of using multiclass classification to automate the identification of patient safety incidents in hospitals...
June 12, 2017: BMC Medical Informatics and Decision Making
https://www.readbyqxmd.com/read/28605205/analysis-of-factors-influencing-drain-amount-time-to-drain-removal-and-seroma-formation-in-patients-undergoing-immediate-expander-implant-breast-reconstruction
#14
Kyong-Je Woo, Joo Myong Paik, Goo-Hyun Mun, Jai-Kyong Pyon, Byung-Joon Jeon, Sa Ik Bang
BACKGROUND: Placement of a closed suction drain is a common cause of patient complaints, such as postoperative pain or discomfort following immediate expander-implant breast reconstruction. This study aims to identify factors that affect drainage volume, time to drain removal, and seroma formation. METHODS: A retrospective chart review of prospectively collected data was conducted on patients who underwent immediate expander-implant breast reconstructions following nipple-sparing or skin-sparing mastectomy without skin excision (nipple areolar complex excision only) between February 2010 and April 2015...
June 12, 2017: Journal of Plastic Surgery and Hand Surgery
https://www.readbyqxmd.com/read/28591297/organization-of-professional-practices-against-intrafamily-violence-against-children-and-adolescents-in-the-institutional-context
#15
Gabriele Schek, Mara Regina Santos da Silva, Carl Lacharité, Maria Emília Nunes Bueno
Objective: to analyze based on the practitioners' discourse, the way they organize their practices confronting situations of intra-family violence against children and adolescents. Method: qualitative research carried out with 15 professionals who work in social and health services located in the southernmost of Brazil. Data were collected through semi-structured interviews, performed at the participants' workplace. We used a theoretical matrix to analyze the data, based on Institutional Ethnography and the technique of discursive textual analysis...
June 5, 2017: Revista Latino-americana de Enfermagem
https://www.readbyqxmd.com/read/28586724/sub-1min-separation-in-sequential-injection-chromatography-for-determination-of-synthetic-water-soluble-dyes-in-pharmaceutical-formulation
#16
Polina Davletbaeva, Petr Chocholouš, Andrey Bulatov, Dalibor Šatínský, Petr Solich
Sequential Injection Chromatography (SIC) evolved from fast and automated non-separation Sequential Injection Analysis (SIA) into chromatographic separation method for multi-element analysis. However, the speed of the measurement (sample throughput) is due to chromatography significantly reduced. In this paper, a sub-1min separation using medium polar cyano monolithic column (5mm×4.6mm) resulted in fast and green separation with sample throughput comparable with non-separation flow methods The separation of three synthetic water-soluble dyes (sunset yellow FCF, carmoisine and green S) was in a gradient elution mode (0...
September 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28558823/first-study-conducted-in-northern-india-that-identifies-group-c-rotavirus-as-the-etiological-agent-of-severe-diarrhea-in-children-in-delhi
#17
Vasundhara Razdan Tiku, Baoming Jiang, Praveen Kumar, Satender Aneja, Arvind Bagga, Maharaj Kishen Bhan, Pratima Ray
BACKGROUND: Group C Rotavirus (RVC) is an enteric pathogen responsible for acute gastroenteritis in children and adults globally. At present there are no surveillance studies on group C Rotaviruses in India and therefore their prevalence in India remains unknown. The present study aimed to evaluate group C rotavirus infection among <5 years old children hospitalized with acute gastroenteritis in New Delhi. METHODS: A total of 350 fecal specimens were collected during September 2013 to November 2014 from <5 years old diarrheal patients admitted at KSCH hospital, Delhi...
May 30, 2017: Virology Journal
https://www.readbyqxmd.com/read/28551536/decision-making-in-irrigation-networks-selecting-appropriate-canal-structures-using-multi-attribute-decision-analysis
#18
Zeinab Hosseinzade, Sheree A Pagsuyoin, Kumaraswamy Ponnambalam, Mohammad J Monem
The stiff competition for water between agriculture and non-agricultural production sectors makes it necessary to have effective management of irrigation networks in farms. However, the process of selecting flow control structures in irrigation networks is highly complex and involves different levels of decision makers. In this paper, we apply multi-attribute decision making (MADM) methodology to develop a decision analysis (DA) framework for evaluating, ranking and selecting check and intake structures for irrigation canals...
May 25, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28550741/exploring-syndrome-differentiation-using-non-negative-matrix-factorization-and-cluster-analysis-in-patients-with-atopic-dermatitis
#19
Younghee Yun, Wonmo Jung, Hyunho Kim, Bo-Hyoung Jang, Min-Hee Kim, Jiseong Noh, Seong-Gyu Ko, Inhwa Choi
Syndrome differentiation (SD) results in a diagnostic conclusion based on a cluster of concurrent symptoms and signs, including pulse form and tongue color. In Korea, there is a strong interest in the standardization of Traditional Medicine (TM). In order to standardize TM treatment, standardization of SD should be given priority. The aim of this study was to explore the SD, or symptom clusters, of patients with atopic dermatitis (AD) using non-negative factorization methods and k-means clustering analysis...
May 22, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28547838/more-efficient-parameter-estimates-for-factor-analysis-of-ordinal-variables-by-ridge-generalized-least-squares
#20
Ke-Hai Yuan, Ge Jiang, Ying Cheng
Data in psychology are often collected using Likert-type scales, and it has been shown that factor analysis of Likert-type data is better performed on the polychoric correlation matrix than on the product-moment covariance matrix, especially when the distributions of the observed variables are skewed. In theory, factor analysis of the polychoric correlation matrix is best conducted using generalized least squares with an asymptotically correct weight matrix (AGLS). However, simulation studies showed that both least squares (LS) and diagonally weighted least squares (DWLS) perform better than AGLS, and thus LS or DWLS is routinely used in practice...
May 26, 2017: British Journal of Mathematical and Statistical Psychology
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