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https://www.readbyqxmd.com/read/29333517/safety-and-feasibility-of-platelet-rich-fibrin-matrix-injections-for-treatment-of-common-urologic-conditions
#1
Ethan L Matz, Amy M Pearlman, Ryan P Terlecki
Purpose: Autologous platelet rich plasma (PRP) is used increasingly in a variety of settings. PRP injections have been used for decades to improve angiogenesis and wound healing. They have also been offered commercially in urology with little to no data on safety or efficacy. PRP could theoretically improve multiple urologic conditions, such as erectile dysfunction (ED), Peyronie's disease (PD), and stress urinary incontinence (SUI). A concern with PRP, however, is early washout, a situation potentially avoided by conversion to platelet rich fibrin matrix (PRFM)...
January 2018: Investigative and Clinical Urology
https://www.readbyqxmd.com/read/29320965/alterations-of-the-gut-microbiota-in-hashimoto-s-thyroiditis-patients
#2
Fuya Zhao, Jing Feng, Jun Li, Lei Zhao, Yang Liu, Huinan Chen, Ye Jin, Biqiang Zhu, Yunwei Wei
BACKGROUND: Hashimoto's thyroiditis (HT) is an organ-specific autoimmune disease in which both genetic predisposition and environmental factors serve as disease triggers. Many studies have indicated that alterations in the gut microbiota are important environmental factors in the development of inflammatory and autoimmune diseases. We systematically performed a comparative analysis of the gut microbiota in HT patients and healthy controls. METHODS: We first conducted a cross-sectional study of 28 HT patients and 16 matched healthy controls...
January 10, 2018: Thyroid: Official Journal of the American Thyroid Association
https://www.readbyqxmd.com/read/29295537/redundancy-analysis-of-capacitance-data-of-a-coplanar-electrode-array-for-fast-and-stable-imaging-processing
#3
Yintang Wen, Zhenda Zhang, Yuyan Zhang, Dongtao Sun
A coplanar electrode array sensor is established for the imaging of composite-material adhesive-layer defect detection. The sensor is based on the capacitive edge effect, which leads to capacitance data being considerably weak and susceptible to environmental noise. The inverse problem of coplanar array electrical capacitance tomography (C-ECT) is ill-conditioning, in which a small error of capacitance data can seriously affect the quality of reconstructed images. In order to achieve a stable image reconstruction process, a redundancy analysis method for capacitance data is proposed...
December 24, 2017: Sensors
https://www.readbyqxmd.com/read/29289302/accurate-anisotropy-recovery-from-fluorophore-mixtures-using-multivariate-curve-resolution-mcr
#4
Yannick Casamayou-Boucau, Alan G Ryder
Anisotropy resolved multidimensional emission spectroscopy (ARMES) provides valuable insights into multi-fluorophore systems like proteins that have complex overlapping emission bands. The method combines multidimensional fluorescence, anisotropy, and chemometrics to facilitate the differentiation of fluorophores with very similar emission properties. Here, we address the critical issue of standardizing the chemometric methods required to accurately extract spectral and anisotropy information from fluorophore mixtures using two standard sample sets: perylene in glycerol, and a mixture of Erythrosin B and Phloxine B with overlapping emission but different anisotropies...
February 13, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29287951/the-effect-of-platelet-rich-fibrin-matrix-at-the-time-of-gluteus-medius-repair-a-retrospective-comparative-study
#5
Bryan M Saltzman, Gift Ukwuani, Eric C Makhni, Jeffrey P Stephens, Shane J Nho
PURPOSE: To evaluate the effect of platelet-rich fibrin matrix (PRFM) on outcomes after surgical repair of gluteus medius tendons. METHODS: This is a retrospective review of prospectively collected data comparing patients who underwent gluteus medius repair with PRFM and patients without PRFM. Preoperative characteristics, intraoperative characteristics, and postoperative outcomes at a minimum of 1 year were recorded. Statistical analysis was performed using a multivariate analysis of variance to test for differences in continuous demographic variables and postoperative-only scores between patient groups, χ2 tests were performed for categorical variables, and a repeated-measures analysis of variance was performed to test for the effects of PRFM...
December 26, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/29273645/stratification-of-knee-osteoarthritis-two-major-patient-subgroups-identified-by-genome-wide-expression-analysis-of-articular-cartilage
#6
Jamie Soul, Sara L Dunn, Sanjay Anand, Ferdinand Serracino-Inglott, Jean-Marc Schwartz, Ray P Boot-Handford, Tim E Hardingham
INTRODUCTION: Osteoarthritis (OA) is a heterogeneous and complex disease. We have used a network biology approach based on genome-wide analysis of gene expression in OA knee cartilage to seek evidence for pathogenic mechanisms that may distinguish different patient subgroups. METHODS: Results from RNA-Sequencing (RNA-Seq) were collected from intact knee cartilage at total knee replacement from 44 patients with OA, from 16 additional patients with OA and 10 control patients with non-OA...
December 22, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/29260858/a-methodology-for-the-validation-of-isotopic-analyses-by-mass-spectrometry-in-stable-isotope-labelling-experiments
#7
Maud Heuillet, Floriant Bellvert, Edern Cahoreau, Fabien Letisse, Pierre Millard, Jean-Charles Portais
Stable-isotope labeling experiments (ILEs) are widely used to investigate the topology and operation of metabolic networks. The quality of isotopic data collected in ILEs is of utmost importance to guarantee the reliability of biological interpretations, but current evaluation approaches are limited due to the lack of a suitable reference material and relevant evaluation criteria. In this work, we present a complete methodology to evaluate mass spectrometry (MS) methods used for quantitative isotopic studies of metabolism...
December 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29251826/digging-deep-into-peptidomics-applied-to-body-fluids
#8
REVIEW
Rui Vitorino
Peptidomics techniques have allowed the identification of thousands of peptides that are derived from proteins in body fluids, despite the considerable challenges behind sample handling, mass spectrometry-based identification, data analysis and integration with bioinformatics tools. Body fluids' naturally occurring peptides are known to perform a variety of local and systemic functions; however, its knowledge is limited. Even so, the biological meaning that can be retrieved from peptidomics applied to the identification of disease markers and to the development of therapies using peptides has driven the progresses made in this field...
December 18, 2017: Proteomics
https://www.readbyqxmd.com/read/29228899/use-and-optimization-of-different-sources-of-information-for-genomic-prediction
#9
Joanna J Ilska, Theo H E Meuwissen, Andreas Kranis, John A Woolliams
BACKGROUND: Molecular data is now commonly used to predict breeding values (BV). Various methods to calculate genomic relationship matrices (GRM) have been developed, with some studies proposing regression of coefficients back to the reference matrix of pedigree-based relationship coefficients (A). The objective was to compare the utility of two GRM: a matrix based on linkage analysis (LA) and anchored to the pedigree, i.e. [Formula: see text] and a matrix based on linkage disequilibrium (LD), i...
December 11, 2017: Genetics, Selection, Evolution: GSE
https://www.readbyqxmd.com/read/29226434/protein-identification-in-imaging-mass-spectrometry-through-spatially-targeted-liquid-micro-extractions
#10
Daniel J Ryan, David Nei, Boone M Prentice, Kristie L Rose, Richard M Caprioli, Jeffrey M Spraggins
RATIONALE: Liquid extraction surface analysis (LESA) can be used to generate spatially-directed protein identifications in an imaging mass spectrometry (IMS) workflow. This approach involves the use of robotic micro-extractions coupled to online liquid chromatography (LC). We have characterized the extraction efficiency of this method as well as its ability to identify proteins from a matrix assisted laser/desorption ionization (MALDI) IMS experiment. METHODS: Proteins and peptides were extracted from transverse sections of a rat brain and sagittal sections of a mouse pup using liquid surface extractions...
December 10, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29223417/left-ventricular-assist-device-impact-on-aortic-valve-mechanics-proteomics-and-ultrastructure
#11
Elizabeth H Stephens, Jiho Han, Emma A Trawick, Elena S Di Martino, Hemanth Akkiraju, Lewis M Brown, Jennifer P Connell, K Jane Grande-Allen, Gordana Vunjak-Novakovic, Hiroo Takayama
BACKGROUND: Aortic regurgitation is a prevalent, detrimental complication of left ventricular assist devices (LVADs). The altered hemodynamics of LVADs results in aortic valves (AVs) having distinct mechanical stimulation. Our hypothesis was that the altered AV hemodynamics modulates the valve cells and matrix, resulting in changes in valvular mechanical properties that then can lead to regurgitation. METHODS: AVs were collected from 16 LVAD and 6 non-LVAD patients at time of heart transplant...
December 6, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/29218249/pericellular-regulation-of-prostate-cancer-expressed-kallikrein-related-peptidases-and-matrix-metalloproteinases-by-cell-surface-serine-proteases
#12
Janet C Reid, Admire Matsika, Claire M Davies, Yaowu He, Amy Broomfield, Nigel C Bennett, Viktor Magdolen, Bhuvana Srinivasan, Judith A Clements, John D Hooper
We provide evidence of a pericellular network of proteases that are elevated and co-expressed in prostate cancer. The network involves the membrane bound serine proteases hepsin and TMPRSS2, the secreted kallikrein-related peptidases KLK4 and KLK14, and the secreted matrix metalloproteinases MMP-3 and MMP-9. Western blot analysis of cell lysates, conditioned cell culture media, immunoprecipitates and cell surface proteins, demonstrates a network of interactions centred largely at the plasma membrane, with the Arg/Lys specific proteases hepsin and TMPRSS2 key regulators of the network...
2017: American Journal of Cancer Research
https://www.readbyqxmd.com/read/29215699/lncrna-linc00880-promotes-cell-proliferation-migration-and-invasion-while-inhibiting-apoptosis-by-targeting-cacng5-through-the-mapk-signaling-pathway-in-spinal-cord-ependymoma
#13
Dong-Mei Wu, Yong-Jian Wang, Xin-Rui Han, Xin Wen, Shan Wang, Min Shen, Shao-Hua Fan, Juan Zhuang, Zi-Feng Zhang, Qun Shan, Meng-Qiu Li, Bin Hu, Chun-Hui Sun, Jun Lu, Yuan-Lin Zheng
The present study was to investigate the effect of lncRNA LINC00880 targeting CACNG5 on cell proliferation, migration, invasion and apoptosis in spinal cord ependymoma (SCE) through the MAPK signaling pathway. GEO database was used to download gene expression data related with SCE (GSE50161 and GSE66354) and annotation file. LncRNA with differential expression was predicted by Multi Experiment Matrix website (MEM). The target gene was analyzed by KEGG pathway enrichment analysis. SCE tissues and adjacent tissues were collected...
December 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29185600/indications-of-comprehensiveness-in-the-pedagogical-relationship-a-design-to-be-constructed-in-nursing-education
#14
Margarete Maria de Lima, Kenya Schmidt Reibnitz, Daiana Kloh, Jussara Gue Martini, Vania Marli Schubert Backes
OBJECTIVE: To analyze how the indications of comprehensiveness translate into the teaching-learning process in a nursing undergraduate course. METHOD: Qualitative case study carried out with professors of a Nursing Undergraduate Course. Data collection occurred through documentary analysis, non-participant observation and individual interviews. Data analysis was guided from an analytical matrix following the steps of the operative proposal. RESULTS: Eight professors participated in the study...
November 27, 2017: Revista da Escola de Enfermagem da U S P
https://www.readbyqxmd.com/read/29185111/factors-influencing-spinal-sagittal-balance-bone-mineral-density-and-oswestry-disability-index-outcome-measures-in-patients-with-rheumatoid-arthritis
#15
Kazutaka Masamoto, Bungo Otsuki, Shunsuke Fujibayashi, Koichiro Shima, Hiromu Ito, Moritoshi Furu, Motomu Hashimoto, Masao Tanaka, Stephen Lyman, Hiroyuki Yoshitomi, Shimei Tanida, Tsuneyo Mimori, Shuichi Matsuda
PURPOSE: To identify the factors influencing spinal sagittal alignment, bone mineral density (BMD), and Oswestry Disability Index (ODI) outcome measures in patients with rheumatoid arthritis (RA). METHODS: We enrolled 272 RA patients to identify the factors influencing sagittal vertical axis (SVA). Out of this, 220 had evaluation of bone mineral density (BMD) and vertebral deformity (VD) on the sagittal plane; 183 completed the ODI questionnaire. We collected data regarding RA-associated clinical parameters and standing lateral X-ray images via an ODI questionnaire from April to December 2012 at a single center...
November 28, 2017: European Spine Journal
https://www.readbyqxmd.com/read/29182017/compressive-detection-of-highly-overlapped-spectra-using-walsh-hadamard-based-filter-functions
#16
Timothy C Corcoran
In the chemometric context in which spectral loadings of the analytes are already known, spectral filter functions may be constructed which allow the scores of mixtures of analytes to be determined in on-the-fly fashion directly, by applying a compressive detection strategy. Rather than collecting the entire spectrum over the relevant region for the mixture, a filter function may be applied within the spectrometer itself so that only the scores are recorded. Consequently, compressive detection shrinks data sets tremendously...
January 1, 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/29179778/determining-requirements-for-patient-centred-care-a-participatory-concept-mapping-study
#17
Kathryn Ogden, Jennifer Barr, David Greenfield
BACKGROUND: Recognition of a need for patient-centred care is not new, however making patient-centred care a reality remains a challenge to organisations. We need empirical studies to extend current understandings, create new representations of the complexity of patient-centred care, and guide collective action toward patient-centred health care. To achieve these ends, the research aim was to empirically determine what organisational actions are required for patient-centred care to be achieved...
November 28, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/29174826/predicting-of-ultrafiltration-performances-by-advanced-data-analysis
#18
B Teychene, A Touffet, J Baron, B Welte, M Joyeux, H Gallard
In order to optimize drinking water production operation, membrane users can use several analytical tools that help membrane fouling prediction and alleviate fouling by a proper feed water resource selection. However, during strong fouling event, membrane decision-makers still face short-term deadline to decide between different options (e.g. optimization of pretreatment or change in feed water quality). Hence, statistical approach might help to better select the most relevant analytical parameter related to fouling potential of a specific resource in order to speed-up decision taking...
November 9, 2017: Water Research
https://www.readbyqxmd.com/read/29173162/oral-fluid-vs-urine-analysis-to-monitor-synthetic-cannabinoids-and-classic-drugs-recent-exposure
#19
Marta Concheiro, Vincent Blandino, Jillian Wetzel, Jiyoung Kim, Petrit Haxhi
BACKGROUND: Urine is a common biological sample to monitor recent drug exposure, and oral fluid is an alternative matrix of increasing interest in clinical and forensic toxicology. Limited data are available about oral fluid vs. urine drug disposition, especially for synthetic cannabinoids. OBJECTIVE: To compare urine and oral fluid as biological matrices to monitor recent drug exposure among HIV-infected homeless individuals. METHODS: Seventy matched urine and oral fluid samples were collected from 13 participants...
November 21, 2017: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/29166708/-effects-of-astragalus-polysaccharide-on-cardiac-dysfunction-in-rabbits-with-severe-scald-injury
#20
L Zhong, J H Zhan, J H Luo, X Cheng
Objective: To investigate the effects of astragalus polysaccharide (AP) on cardiac dysfunction in rabbits with severe scald injury. Methods: Sixty-four New Zealand white rabbits were divided into pure scald group and AP group according to the random number table, with 32 rabbits in each group. Rabbits in the two groups were all inflicted with 30% total body surface area full-thickness scald on the back. Immediately after injury, rabbits in two groups were intraperitoneally injected with lactated Ringer's solution once for antishock...
November 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
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