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https://www.readbyqxmd.com/read/28516351/analysis-of-some-metallic-elements-and-metalloids-composition-and-relationships-in-parasol-mushroom-macrolepiota-procera
#1
Jerzy Falandysz, Atindra Sapkota, Anna Dryżałowska, Małgorzata Mędyk, Xinbin Feng
The aim of the study was to characterise the multi-elemental composition and associations between a group of 32 elements and 16 rare earth elements collected by mycelium from growing substrates and accumulated in fruiting bodies of Macrolepiota procera from 16 sites from the lowland areas of Poland. The elements were quantified by inductively coupled plasma quadrupole mass spectrometry using validated method. The correlation matrix obtained from a possible 48 × 16 data matrix has been used to examine if any association exits between 48 elements in mushrooms foraged from 16 sampling localizations by multivariate approach using principal component (PC) analysis...
May 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28509694/does-the-use-of-acellular-dermal-matrix-increase-postoperative-complications-of-the-first-stage-reconstruction-of-immediate-expander-implant-breast-reconstruction-a-matched-cohort-study
#2
Kyong-Je Woo, Jin-Woo Park, Goo-Hyun Mun, Jai-Kyong Pyon, Byung-Joon Jeon, Sa Ik Bang
PURPOSE: Controversy exists regarding complications associated with the use of acellular dermal matrix (ADM). This likely stems from the heterogeneous and unmatched patient characteristics in study groups. The purpose of this study was to analyze complications in a matched cohort to identify whether ADM use increased postoperative complications of the first-stage immediate expander-implant breast reconstructions. METHODS: A retrospective matched-cohort study was performed...
May 15, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28483139/job-stickiness-of-young-nurses-in-ontario-does-the-employer-organization-participation-in-the-nursing-graduate-guarantee-initiative-make-a-difference
#3
Mohamad Alameddine, Andrea Baumann, Kanecy Onate, Mary Crea, Nour El Arnaout, Raisa Deber
This study assesses the long-term impact of the Nursing Graduate Guarantee (NGG) initiative using the concept of "stickiness" to examine the employment trends of young nurses in Ontario and evaluate the effect of employers' participation in the NGG on the full-time (FT) employment patterns of young nurses and whether this effect varies by category of nurse and sector of employment. A quantitative analysis of a de-identified linked subset of the College of Nurses of Ontario nursing registration database for years 2000-2014 and data collected from the NGG employment portal for years 2007-2014 was performed...
April 28, 2017: Health Policy
https://www.readbyqxmd.com/read/28480136/the-earliest-known-titanosauriform-sauropod-dinosaur-and-the-evolution-of-brachiosauridae
#4
Philip D Mannion, Ronan Allain, Olivier Moine
Brachiosauridae is a clade of titanosauriform sauropod dinosaurs that includes the well-known Late Jurassic taxa Brachiosaurus and Giraffatitan. However, there is disagreement over the brachiosaurid affinities of most other taxa, and little consensus regarding the clade's composition or inter-relationships. An unnamed partial sauropod skeleton was collected from middle-late Oxfordian (early Late Jurassic) deposits in Damparis, in the Jura department of eastern France, in 1934. Since its brief description in 1943, this specimen has been informally known in the literature as the 'Damparis sauropod' and 'French Bothriospondylus', and has been considered a brachiosaurid by most authors...
2017: PeerJ
https://www.readbyqxmd.com/read/28437133/the-impact-of-the-centrifuge-characteristics-and-centrifugation-protocols-on-the-cells-growth-factors-and-fibrin-architecture-of-a-leukocyte-and-platelet-rich-fibrin-l-prf-clot-and-membrane
#5
David M Dohan Ehrenfest, Nelson R Pinto, Andrea Pereda, Paula Jiménez, Marco Del Corso, Byung-Soo Kang, Mauricio Nally, Nicole Lanata, Hom-Lay Wang, Marc Quirynen
L-PRF (leukocyte- and platelet-rich fibrin) is one of the four families of platelet concentrates for surgical use and is widely used in oral and maxillofacial regenerative therapies. The first objective of this article was to evaluate the mechanical vibrations appearing during centrifugation in four models of commercially available table-top centrifuges used to produce L-PRF and the impact of the centrifuge characteristics on the cell and fibrin architecture of a L-PRF clot and membrane. The second objective of this article was to evaluate how changing some parameters of the L-PRF protocol may influence its biological signature, independently from the characteristics of the centrifuge...
April 24, 2017: Platelets
https://www.readbyqxmd.com/read/28436032/application-of-maldi-tof-ms-for-estimating-the-postmortem-interval-in-rat-muscle-samples
#6
Chengzhi Li, Dong Ma, Kaifei Deng, Yijiu Chen, Ping Huang, Zhenyuan Wang
Estimating the postmortem interval (PMI) is very important in the forensic sciences. Although many approaches have been used for estimating the PMI, accurate PMI calculations are still difficult. In this study, four Sprague Dawley (SD) rats were sacrificed by suffocation, and muscle samples were collected by dissection at various time intervals (0, 48, 96, and 144 h) after death. All samples were probed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) to obtain molecular images and data for principal component analysis (PCA)...
April 23, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28433026/variational-koopman-models-slow-collective-variables-and-molecular-kinetics-from-short-off-equilibrium-simulations
#7
Hao Wu, Feliks Nüske, Fabian Paul, Stefan Klus, Péter Koltai, Frank Noé
Markov state models (MSMs) and master equation models are popular approaches to approximate molecular kinetics, equilibria, metastable states, and reaction coordinates in terms of a state space discretization usually obtained by clustering. Recently, a powerful generalization of MSMs has been introduced, the variational approach conformation dynamics/molecular kinetics (VAC) and its special case the time-lagged independent component analysis (TICA), which allow us to approximate slow collective variables and molecular kinetics by linear combinations of smooth basis functions or order parameters...
April 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28422971/comparison-of-chemical-use-between-hydraulic-fracturing-acidizing-and-routine-oil-and-gas-development
#8
COMPARATIVE STUDY
William T Stringfellow, Mary Kay Camarillo, Jeremy K Domen, Seth B C Shonkoff
The potential hazards and risks associated with well-stimulation in unconventional oil and gas development (hydraulic fracturing, acid fracturing, and matrix acidizing) have been investigated and evaluated and federal and state regulations requiring chemical disclosure for well-stimulation have been implemented as part of an overall risk management strategy for unconventional oil and gas development. Similar evaluations for chemicals used in other routine oil and gas development activities, such as maintenance acidizing, gravel packing, and well drilling, have not been previously conducted, in part due to a lack of reliable information concerning on-field chemical-use...
2017: PloS One
https://www.readbyqxmd.com/read/28412350/patient-sample-oriented-analysis-of-gene-expression-highlights-extracellular-signatures-in-breast-cancer-progression
#9
Yourae Hong, Nayoung Kim, Chao Li, Euna Jeong, Sukjoon Yoon
Although a large collection of cancer cell lines are useful surrogates for patient samples, the physiological relevance of observed molecular phenotypes in cell lines remains controversial. Because transcriptome data are a representative set of molecular phenotypes in cancers, we systematically analyzed the discrepancy of global gene expression profiles between patient samples and cell lines in breast cancers. While the majority of genes exhibited general consistency between patient samples and cell lines, the expression of genes in the categories of extracellular matrix, collagen trimers, receptor activity, catalytic activity and transporter activity were significantly up-regulated only in tissue samples...
April 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28410607/aprecisekure-an-approach-of-precision-medicine-in-a-rare-disease
#10
Ottavia Spiga, Vittoria Cicaloni, Andrea Bernini, Andrea Zatkova, Annalisa Santucci
BACKGROUND: Alkaptonuria (AKU; OMIM:203500) is a classic Mendelian genetic disorder described by Garrod already in 1902. It causes urine to turn black upon exposure to air and also leads to ochronosis as well as early osteoarthritis. Our objective is the implementation of a Precision Medicine (PM) approach to AKU. We present here a novel ApreciseKUre database facilitating the collection, integration and analysis of patient data in order to create an AKU-dedicated "PM Ecosystem" in which genetic, biochemical and clinical resources can be shared among registered researchers...
April 14, 2017: BMC Medical Informatics and Decision Making
https://www.readbyqxmd.com/read/28407189/molecular-analysis-of-genetic-diversity-among-vine-accessions-using-dna-markers
#11
A F da Costa, P E Teodoro, L L Bhering, F D Tardin, R F Daher, W F Campos, A P Viana, M G Pereira
Viticulture presents a number of economic and social advantages, such as increasing employment levels and fixing the labor force in rural areas. With the aim of initiating a program of genetic improvement in grapevine from the State University of the state of Rio de Janeiro North Darcy Ribeiro, genetic diversity between 40 genotypes (varieties, rootstock, and species of different subgenera) was evaluated using Random amplified polymorphic DNA (RAPD) molecular markers. We built a matrix of binary data, whereby the presence of a band was assigned as "1" and the absence of a band was assigned as "0...
April 13, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28401407/molecular-analysis-of-amantadine-resistant-influenza-a-h1n1-pdm09-virus-isolated-from-slum-dwellers-of-dhaka-bangladesh
#12
Mozibur Rahman, Sheikh Ariful Hoque, Mohammad Ariful Islam, Sabita Rezwana Rahman
Influenza is a highly contagious viral infection associated with excessive hospitalizations and deaths throughout the world. Continuous antigenic shift and drift is not only responsible for this devastating effect of influenza but also causes ineffectiveness of antiviral drugs and vaccines. In this study, we investigated the effectiveness of ribavirin, oseltamivir, and amantadine drugs in vitro against nine influenza A isolates collected during June 2012-August 2013 from different slums in Dhaka city. The effectiveness of these drugs was determined by measuring the inhibition of virus-induced cytopathic effect on MDCK cells through MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)...
June 2017: Virus Genes
https://www.readbyqxmd.com/read/28398127/near-infrared-spectroscopy-predicts-compositional-and-mechanical-properties-of-hyaluronic-acid-based-engineered-cartilage-constructs
#13
Farzad Yousefi, Minwook Kim, Syeda Yusra Nahri, Robert Leon Mauck, Nancy Pleshko
Hyaluronic acid (HA) has been widely used for cartilage tissue engineering applications. However, the optimal time point to harvest HA-based engineered constructs for cartilage repair is still under investigation. Here, we investigated the ability of a non-destructive modality, near infrared spectroscopic (NIR) analysis, to predict compositional and mechanical properties of HA-based engineered cartilage constructs. NIR spectral data were collected from control, unseeded constructs, and twice per week via fiber optic from constructs seeded with chondrocytes during their development over an 8 week period...
April 11, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28379208/analysis-of-optimal-sensor-positions-for-activity-classification-and-application-on-a-different-data-collection-scenario
#14
Natthapon Pannurat, Surapa Thiemjarus, Ekawit Nantajeewarawat, Isara Anantavrasilp
This paper focuses on optimal sensor positioning for monitoring activities of daily living and investigates different combinations of features and models on different sensor positions, i.e., the side of the waist, front of the waist, chest, thigh, head, upper arm, wrist, and ankle. Nineteen features are extracted, and the feature importance is measured by using the Relief-F feature selection algorithm. Eight classification algorithms are evaluated on a dataset collected from young subjects and a dataset collected from elderly subjects, with two different experimental settings...
April 5, 2017: Sensors
https://www.readbyqxmd.com/read/28370605/metabolite-mapping-by-consecutive-nanostructure-and-silver-assisted-mass-spectrometry-imaging-on-tissue-sections
#15
O J R Gustafsson, T M Guinan, D Rudd, H Kobus, K Benkendorff, N H Voelcker
RATIONALE: Nanostructure-based mass spectrometry imaging (MSI) is a promising technology for molecular imaging of small molecules, without the complex chemical background typically encountered in matrix-assisted molecular imaging approaches.Here, we have enhanced these surfaces with silver (Ag) to provide a second tier of MSI data from a single sample. METHODS: MSI data was acquired through the application of laser desorption/ionization mass spectrometry to biological samples imprinted onto desorption/ionisation on silicon (DIOS) substrates...
March 31, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28369339/hic-spector-a-matrix-library-for-spectral-and-reproducibility-analysis-of-hi-c-contact-maps
#16
Koon-Kiu Yan, Galip Gürkan Yardimci, Chengfei Yan, William S Noble, Mark Gerstein
Summary: Genome-wide proximity ligation based assays like Hi-C have opened a window to the 3D organization of the genome. In so doing, they present data structures that are different from conventional 1D signal tracks. To exploit the 2D nature of Hi-C contact maps, matrix techniques like spectral analysis are particularly useful. Here, we present HiC-spector, a collection of matrix-related functions for analyzing Hi-C contact maps. In particular, we introduce a novel reproducibility metric for quantifying the similarity between contact maps based on spectral decomposition...
March 22, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28367546/delineation-of-disease-phenotypes-associated-with-esophageal-adenocarcinoma-by-maldi-ims-ms-analysis-of-serum-n-linked-glycans
#17
M M Gaye, T Ding, H Shion, A Hussein, Y Hu, S Zhou, Z T Hammoud, B K Lavine, Y Mechref, J C Gebler, D E Clemmer
N-Linked glycans, extracted from patient sera and healthy control individuals, are analyzed by Matrix-assisted laser desorption ionization (MALDI) in combination with ion mobility spectrometry (IMS), mass spectrometry (MS) and pattern recognition methods. MALDI-IMS-MS data were collected in duplicate for 58 serum samples obtained from individuals diagnosed with Barrett's esophagus (BE, 14 patients), high-grade dysplasia (HGD, 7 patients), esophageal adenocarcinoma (EAC, 20 patients) and disease-free control (NC, 17 individuals)...
May 2, 2017: Analyst
https://www.readbyqxmd.com/read/28357075/de-novo-systemic-deleterious-amino-acid-substitutions-are-common-in-large-cytoskeleton-related-protein-coding-regions
#18
Rebecca J Stoll, Grace R Thompson, Mohammad D Samy, George Blanck
Human mutagenesis is largely random, thus large coding regions, simply on the basis of probability, represent relatively large mutagenesis targets. Thus, we considered the possibility that large cytoskeletal-protein related coding regions (CPCRs), including extra-cellular matrix (ECM) coding regions, would have systemic nucleotide variants that are not present in common SNP databases. Presumably, such variants arose recently in development or in recent, preceding generations. Using matched breast cancer and blood-derived normal datasets from the cancer genome atlas, CPCR single nucleotide variants (SNVs) not present in the All SNPs(142) or 1000 Genomes databases were identified...
February 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28351133/monitoring-the-behavior-of-emerging-contaminants-in-wastewater-impacted-rivers-based-on-the-use-of-fluorescence-excitation-emission-matrixes-eem
#19
Massimiliano Sgroi, Paolo Roccaro, Gregory V Korshin, Federico G A Vagliasindi
This study investigated the applicability of fluorescence indexes based on the interpretation of excitation emission matrices (EEMs) by PARAFAC analysis and by selecting fluorescence intensities at a priori defined excitation/emission pairs as surrogates for monitoring the behavior of emerging organic compounds (EOCs) in two catchment basins impacted by wastewater discharges. Relevant EOC and EEM data were obtained for a 90 km stretch of the Simeto River, the main river in Sicily, and the smaller San Leonardo River, which was investigated for a 17 km stretch...
April 6, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28340744/simultaneous-multi-residue-determination-of-twenty-one-veterinary-drugs-in-poultry-litter-by-modeling-three-way-liquid-chromatography-with-fluorescence-and-absorption-detection-data
#20
Carla M Teglia, Paola M Peltzer, Silvia N Seib, Rafael C Lajmanovich, María J Culzoni, Héctor C Goicoechea
A method for the simultaneous investigation of twenty one veterinary active ingredients in poultry litter based on MCR-ALS modeling of three-way liquid chromatography with fluorescence and UV detection data is presented. The chromatographic procedure was optimized in terms of both the nature of the organic solvent and the pH of the mobile phase to maximize the resolution of the analytes. In order to improve the simultaneous extraction efficiency of the twenty one veterinary drugs, a simplex-centroid design with combinations of the three components of the extracting mixture, i...
May 15, 2017: Talanta
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