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https://www.readbyqxmd.com/read/27910758/antimicrobial-coumarins-from-the-oyster-culinary-medicinal-mushroom-pleurotus-ostreatus-agaricomycetes-from-kenya
#1
Lilechi D Baraza, Wekesa Neser, Korir Cheruiyot Jackson, Juma B Fredrick, Ochieno Dennis, Kamau R Wairimu, Aggrey Osogo Keya, Matthias Heydenreich
Pleurotus ostreatus has been widely used as food because of its nutritional and medicinal properties. These have been attributed to the presence of macronutrients, minerals, vitamins, and amino acids, among other secondary metabolites. There are, however, few reports on the antimicrobial activities of different classes of purified compounds from P. ostreatus. This led to the current study, the objective of which was to chemically characterize the antibiotic activities of P. ostreatus against selected human pathogenic bacteria and endophytic fungi...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27910702/a-new-isoquinolone-alkaloid-from-an-endophytic-fungus-r22-of-nerium-indicum
#2
Yang-Min Ma, Ke Qiao, Yang Kong, Meng-Yun Li, Lin-Xin Guo, Zhi Miao, Chao Fan
A new isoquinolone alkaloid named 5-hydroxy-8-methoxy-4-phenylisoquinolin-1(2H)-one (3), together with two known quinolinone alkaloids 3-O-methylviridicatin (1) and viridicatol (2) were isolated from the fermentation of an endophytic fungus Penicillium sp. R22 in Nerium indicum. Their structures were elucidated by NMR, IR and MS data, and were also confirmed by comparing with the reported data in the literature. Meanwhile, the antibacterial and antifungal activities of all compounds were tested, and the results showed that three compounds had strong antifungal activity...
December 2, 2016: Natural Product Research
https://www.readbyqxmd.com/read/27910191/tintelnotia-a-new-genus-in-phaeosphaeriaceae-harbouring-agents-of-cornea-and-nail-infections-in-humans
#3
S A Ahmed, W Hofmüller, M Seibold, G S de Hoog, H Harak, I Tammer, A D van Diepeningen, W Behrens-Baumann
Phaeosphaeriaceae is a family in the order Pleosporales containing numerous plant pathogens, endophytes, lichenised fungi, and environmental saprobes. A novel genus, Tintelnotia is introduced containing two species, one of which caused an eye infection and several nail infections in humans. All species of Tintelnotia produce conidia in soft pycnidia with a wide ostiole. The generic type species is T. opuntiae causing necrotic spots on cactus plants. The isolates of the human opportunist T. destructans showed variable susceptibility pattern to a panel of common antifungal agents...
December 1, 2016: Mycoses
https://www.readbyqxmd.com/read/27902318/streptomyces-capparidis-sp-nov-a-novel-endophytic-actinobacterium-isolated-from-fruits-of-capparis-spinosa-l
#4
Hong-Fei Wang, Qiu-Li Li, Min Xiao, Yong-Guang Zhang, Xing-Kui Zhou, Manik Prabhu Narsing Rao, Yan-Qing Duan, Wen-Jun Li
A novel endophytic actinobactrium strain, designated EGI 6500195T, was isolated from Capparis spinosa. Growth occurred at 10-45 °C (optimum 30 °C), pH 6-8 (optimum pH 7) and in the presence of 0-1% NaCl (w/v). The strain EGI 6500195T shared the highest 16S rRNA gene sequence similarity (97.74 %) with Streptomyces vitaminophilus DSM 41686T and less than 97% sequence similarities with other members of the genus Streptomyces. The diagnostic amino acid in the peptidoglycan was LL-diaminopimelic acid. Whole-cell hydrolysates contained glucose, ribose,fructose and mannose...
October 18, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902296/allostreptomyces-psammosilenae-gen-nov-sp-nov-an-endophytic-actinobacterium-isolated-from-the-roots-of-psammosilene-tunicoides-and-emended-description-of-the-family-streptomycetaceae-waksman-and-henrici-1943-al-emend-rainey-et-al-1997-emend-kim-et-al-2003-emend
#5
Mei-Juan Huang, Manik Prabhu Narsing Rao, Nimaichand Salam, Min Xiao, Hai-Quan Huang, Wen-Jun Li
A Gram-staining positive actinobacterium, designated strain YIM DR4008T was isolated from the root sample of Psammosilene tunicoides collected from Lijiang, Yunnan, China. Strain YIM DR4008T could grow at temperature ranging from 10 to 50 °C (optimum at 28-30 °C), pH 5.0-11.0 (optimum pH 7.0) and in the presence of up to 4 % (w/v) NaCl. Sequence analysis of the 16S ribosomal RNA gene revealed that strain YIM DR4008T shared highest similarity (95.0 %) with Streptomyces griseoplanus NBRC 12779T and < 95 % similarity with other known members of the genera Streptomyces, Kitasatospora and Streptacidiphilus...
October 27, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902284/chryseobacterium-cucumeris-sp-nov-an-endophyte-isolated-from-cucumber-cucumis-sativus-l-root-and-emended-description-of-chryseobacterium-arthrosphaerae
#6
Jin-Ju Jeong, Dong Wan Lee, Byeonghyeok Park, Mee Kyung Sang, In-Geol Choi, Ki Deok Kim
The Gram-stain-negative, yellow-pigmented, rod-shaped bacterial strain GSE06T, isolated from the surface-sterilized root of a cucumber plant grown in a field in Gunsan, Korea, was characterized by not only cultural and morphological features but also physiological, biochemical, and molecular analyses. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain GSE06T was most related to species of the genus Chryseobacterium. Furthermore, strain GSE06T exhibited the highest sequence similarities with type strains of Chryseobacterium indologenes ATCC 29897T (98...
November 17, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902278/chryseobacterium-endophyticum-sp-nov-isolated-from-a-maize-leaf
#7
Shih-Yao Lin, Asif Hameed, You-Cheng Liu, Yi-Han Hsu, Yu-Ting Hsieh, Wei-An Lai, Chiu-Chung Young
A Gram-stain-negative, aerobic, yellow-pigmented, flexirubin-producing, rod-shaped and endophytic bacterium, designated strain CC-YTH209T, was isolated from a maize leaf and subjected to a taxonomic study. Strain CC-YTH209T was found to grow at 15-40 °C (optimal 30 °C), at pH 6.0-8.0 (optimal pH 7.0) and in the presence of 0-2 % (optimal 1 %) (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain CC-YTH209T appeared to belong to the genus Chryseobacterium within the class Flavobacteriia, and was closely related to Chryseobacterium rigui CJ16T (97...
November 11, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902277/pedobacter-zeae-sp-nov-an-endophytic-bacterium-isolated-from-maize-root
#8
Jun-Lian Gao, Pengbo Sun, Xiao-Jie Mao, Yan-Li Du, Bing-Yan Liu, Jian-Guang Sun
A novel Gram-staining-negative, aerobic, non-motile by gliding and rod-shaped strain designated 22T was isolated from surface-sterilized root tissue of maize planted in the Fangshan District of Beijing, People's Republic of China. The highest levels of 16S rRNA gene sequence similarity were found with respect to Pedobacter suwonensis15-52T (97.5 %), Pedobacter terrae DS-57T (97.1 %) and Pedobacter alluvionis NWER-II11T (97.0 %). Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain 22T is a member of the genus Pedobacter...
October 25, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902235/micromonospora-parathelypteridis-sp-nov-a-novel-endophytic-actinomycete-with-antifungal-activity-isolated-from-the-root-of-parathelypteris-beddomei-bak-ching
#9
Shanshan Zhao, Chongxi Liu, Weiwei Zheng, Zhaoxu Ma, Tingting Cao, Junwei Zhao, Kai Yan, Wensheng Xiang, Xiangjing Wang
A novel endophytic actinomycete with antifungal activity, designated strain NEAU-JXY5T, was isolated from the root of Parathelypteris beddomei (Bak.) Ching. Strain NEAU-JXY5T showed closest 16S rRNA gene sequence similarity to Micromonospora luteifusca GUI2T (99.31 %), and phylogenetically clustered with Micromonospora noduli GUI43T (99.24 %), Micromonospora lycii NEAU-gq11T (99.19 %), Micromonospora zeae NEAU-gq9T (99.12 %), Micromonospora saelicesensis Lupac 09T (98.97 %), Micromonospora vinacea GUI63T (98...
October 26, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902223/bacillus-capparidis-sp-nov-a-novel-endophytic-bacterium-isolated-from-roots-of-capparis-spinosa-l
#10
Hong-Fei Wang, Qiu-Li Li, Yong-Guang Zhang, Min Xiao, Xing-Kui Zhou, Jian-Wei Guo, Yan-Qing Duan, Wen-Jun Li
A novel endophytic bacterium, designated strain EGI 6500252T, was isolated from the surface-sterilized roots of medicinal plant (Capparis spinosa L.) collected from Urumqi city, Xinjiang, north-west of China. Cells were Gram-staining-positive, non-motile, aerobic, catalase- and oxidase-positive, rod-shaped and did not display spore formation. Strain EGI 6500252T grew at 10-40 °C (optimum 25-30 °C) and pH 6.0-8.0 (optimum pH 7.0), and in the presence of 0-10 % NaCl (w/v) (optimum 0-3 %). The major cellular fatty acids (> 10 %) were identified as iso-C15:0, anteiso-C15:0, anteiso-C17:0 and Sum In feature 4...
October 26, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27901067/a-chitin-deacetylase-from-the-endophytic-fungus-pestalotiopsis-sp-efficiently-inactivates-the-elicitor-activity-of-chitin-oligomers-in-rice-cells
#11
Stefan Cord-Landwehr, Rebecca L J Melcher, Stephan Kolkenbrock, Bruno M Moerschbacher
To successfully survive in plants, endophytes need strategies to avoid being detected by the plant immune system, as the cell walls of endophytes contain easily detectible chitin. It is possible that endophytes "hide" this chitin from the plant immune system by modifying it, or oligomers derived from it, using chitin deacetylases (CDA). To explore this hypothesis, we identified and expressed a CDA from Pestalotiopsis sp. (PesCDA), an endophytic fungus, in E. coli and characterized this enzyme and its chitosan oligomer products...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27900685/assessing-bacterial-and-fungal-diversity-in-the-plant-endosphere
#12
Bernd Wemheuer, Franziska Wemheuer
Plants are colonized various microorganisms including endophytes. These microbes can play an important role in agricultural production as they promote plant growth and/or enhance the resistance of their host plant against diseases and environmental stress conditions. Although culture-independent molecular approaches such as DNA barcoding have greatly enhanced our understanding of bacterial and fungal endophyte communities, there are some methodical problems when investigating endophyte diversity. One main issue are sequence contaminations such as plastid-derived rRNA gene sequences which are co-amplified due to their high homology to bacterial 16S rRNA genes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27900468/complete-genome-sequence-of-a-variant-of-maize-associated-totivirus-from-ecuador
#13
Robert A Alvarez-Quinto, Rodrigo F Espinoza-Lozano, Carlos A Mora-Pinargote, Diego F Quito-Avila
The complete genomic sequence of a variant of the recently reported maize-associated totivirus (MATV) from China was obtained from commercial maize in Ecuador. The genome of MATV-Ec (Ecuador) (4,998 bp) is considerably longer than that of MATV-Ch (China) (3,956 bp), the main difference due to a ≈ 1-kb-long capsid-protein-encoding fragment that is completely absent from the Chinese genome. Sequence alignments between MATV-Ec and MATV-Ch showed an overall identity of 82% at the nucleotide level, whereas at the amino acid level, the viruses exhibited 95% and 94% identity for the putative capsid protein and the RNA-dependent RNA polymerase (RdRp), respectively...
November 30, 2016: Archives of Virology
https://www.readbyqxmd.com/read/27897983/effects-of-polysaccharide-elicitors-from-endophytic-fusarium-oxysporum-fat9-on-the-growth-flavonoid-accumulation-and-antioxidant-property-of-fagopyrum-tataricum-sprout-cultures
#14
Lingyun Zhong, Bei Niu, Lin Tang, Fang Chen, Gang Zhao, Jianglin Zhao
The purpose of this study was to evaluate the effects of four different fungal polysaccharides, named water-extracted mycelia polysaccharide (WPS), sodium hydroxide-extracted mycelia polysaccharide (SPS), hydrochloric-extracted mycelia polysaccharide (APS), and exo-polysaccharide (EPS) obtained from the endophytic Fusarium oxysporum Fat9 on the sprout growth, flavonoid accumulation, and antioxidant capacity of tartary buckwheat. Without visible changes in the appearance of the sprouts, the exogenous polysaccharide elicitors strongly stimulated sprout growth and flavonoid production, and the stimulation effect was closely related with the polysaccharide (PS) species and its treatment dosage...
November 25, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27897975/polyketides-with-immunosuppressive-activities-from-mangrove-endophytic-fungus-penicillium-sp-zj-sy%C3%A2
#15
Hongju Liu, Senhua Chen, Weiyang Liu, Yayue Liu, Xishan Huang, Zhigang She
Nine polyketides, including two new benzophenone derivatives, peniphenone (1) and methyl peniphenone (2), along with seven known xanthones (3-9) were obtained from mangrove endophytic fungus Penicillium sp. ZJ-SY₂ isolated from the leaves of Sonneratia apetala. Their structures were elucidated on the basis of MS, 1D, and 2D NMR data. Compounds 1, 3, 5, and 7 showed potent immunosuppressive activity with IC50 values ranging from 5.9 to 9.3 μg/mL.
November 25, 2016: Marine Drugs
https://www.readbyqxmd.com/read/27896581/secondary-metabolites-of-endophytic-xylaria-species-with-potential-applications-in-medicine-and-agriculture
#16
REVIEW
Martha Lydia Macías-Rubalcava, Rosa Elvira Sánchez-Fernández
Fungal endophytes are important sources of bioactive secondary metabolites. The genus Xylaria Hill (ex Schrank, 1789, Xylariaceae) comprises various endophytic species associated to both vascular and non vascular plants. The secondary metabolites produced by Xylaria species include a variety of volatile and non-volatile compounds. Examples of the former are sesquiterpenoids, esters, and alcohols, among others; and of the latter we find terpenoids, cytochalasins, mellein, alkaloids, polyketides, and aromatic compounds...
January 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27895938/understanding-colonization-and-proliferation-potential-of-endophytes-and-pathogen-in-planta-via-plating-polymerase-chain-reaction-and-ergosterol-assay
#17
Yiing Yng Chow, Sadequr Rahman, Adeline Su Yien Ting
This study aimed to establish the colonization behavior and proliferation potential of three endophytes and one pathogen Ganoderma boninense (Gb) introduced into oil palm ramets (host model). The endophytes selected were Diaporthe phaseolorum (WAA02), Trichoderma asperellum (T2), and Penicillium citrinum (BTF08). Ramets were first inoculated with 100 mL of fungal cells (10(6) cfu mL(-)(1)) via soil drenching. For the next 7 days, ramets were sampled and subjected to three different assays to detect and identify fungal colonization, and establish their proliferation potential in planta...
January 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/27895623/molecular-approaches-to-screen-bioactive-compounds-from-endophytic-fungi
#18
REVIEW
M Vasundhara, Anil Kumar, M Sudhakara Reddy
Endophytic fungi are capable of producing plant associated metabolites and their analogs with therapeutic value. In order to identify the potential endophytic isolates producing bioactive compounds, one need to screen all isolated endophytes, which may run into hundreds. Isolation of endophytic fungi is relatively a simple process; but screening of the isolated fungi for required metabolite production is a cumbersome process. Endophytic fungi producing plant associated metabolites may contain genes involved in the entire biosynthetic pathway(s)...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27893193/the-oil-contaminated-soil-diazotroph-azoarcus-olearius-dqs-4-t-is-genetically-and-phenotypically-similar-to-the-model-grass-endophyte-azoarcus-sp-bh72
#19
Helisson Faoro, Rodrigo Rene Menegazzo, Federico Battistoni, Prasad Gyaneshwar, Fernanda P do Amaral, Cecilia Taulé, Sydnee Rausch, Patricia Gonçalves Galvão, Cecilia de Los Santos, Shubhajit Mitra, Gabriela Heijo, Shih-Yi Sheu, Wen-Ming Chen, Cintia Mareque, Michelle Zibetti Tadra-Sfeir, J Ivo Baldani, Marta Maluk, Ana Paula Guimarães, Gary Stacey, Emanuel M de Souza, Fabio O Pedrosa, Leonardo Magalhães Cruz, Euan K James
The genome of Azoarcus olearius DQS-4(T) , a N2 -fixing Betaproteobacterium isolated from oil-contaminated soil in Taiwan, was sequenced and compared with other Azoarcus strains. The genome sequence showed high synteny with Azoarcus sp. BH72, a model endophytic diazotroph, but low synteny with five non-plant-associated strains (Azoarcus CIB, Azoarcus EBN1, Azoarcus KH32C, A. toluclasticus MF63(T) and Azoarcus PA01). Average Nucleotide Identity (ANI) revealed that DQS-4(T) shares 98.98% identity with Azoarcus BH72, which should now be included in the species A...
November 28, 2016: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/27892688/secondary-metabolites-from-colletotrichum-capsici-an-endophytic-fungus-derived-from-siegesbeckia-pubescens-makino
#20
Fuqian Wang, Haoran Ma, Zhengxi Hu, Jie Jiang, Hucheng Zhu, Lu Cheng, Quanwei Yang, Hong Zhang, Geng Zhang, Yonghui Zhang
A rare new tremulane sesquiterpenoid analogue, 11,12-epoxy-5,6-seco-1,6(13)-tremuladien-5,12-olide (1), together with five known altenuene derivatives (2-6) was isolated from the cultures of Colletotrichum capsici, which was isolated as an endophytic fungus from fresh leaves of Siegesbeckia pubescens Makino (Compositae). Their structures were elucidated by means of spectroscopic methods and comparison with literature data. All compounds isolated were reported for the first time from the fungus C. capsici.
November 28, 2016: Natural Product Research
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