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https://www.readbyqxmd.com/read/29351296/hybrid-genome-assembly-and-annotation-of-paenibacillus-pasadenensis-strain-r16-reveals-insights-on-endophytic-life-style-and-antifungal-activity
#1
Alessandro Passera, Luca Marcolungo, Paola Casati, Milena Brasca, Fabio Quaglino, Chiara Cantaloni, Massimo Delledonne
Bacteria of the Paenibacillus genus are becoming important in many fields of science, including agriculture, for their positive effects on the health of plants. However, there are little information available on this genus compared to other bacteria (such as Bacillus or Pseudomonas), especially when considering genomic information. Sequencing the genomes of plant-beneficial bacteria is a crucial step to identify the genetic elements underlying the adaptation to life inside a plant host and, in particular, which of these features determine the differences between a helpful microorganism and a pathogenic one...
2018: PloS One
https://www.readbyqxmd.com/read/29351192/seed-endophyte-microbiome-of-crotalaria-pumila-unpeeled-identification-of-plant-beneficial-methylobacteria
#2
Ariadna S Sánchez-López, Isabel Pintelon, Vincent Stevens, Valeria Imperato, Jean-Pierre Timmermans, Carmen González-Chávez, Rogelio Carrillo-González, Jonathan Van Hamme, Jaco Vangronsveld, Sofie Thijs
Metal contaminated soils are increasing worldwide. Metal-tolerant plants growing on metalliferous soils are fascinating genetic and microbial resources. Seeds can vertically transmit endophytic microorganisms that can assist next generations to cope with environmental stresses, through yet poorly understood mechanisms. The aims of this study were to identify the core seed endophyte microbiome of the pioneer metallophyte Crotalaria pumila throughout three generations, and to better understand the plant colonisation of the seed endophyte Methylobacterium sp...
January 19, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29348531/isopentylated-diphenyl-ether-derivatives-from-the-fermentation-products-of-an-endophytic-fungus-phomopsis-fukushii
#3
Zhen-Jie Li, Hai-Ying Yang, Jing Li, Xin Liu, Lin Ye, Wei-Song Kong, Shi-Yun Tang, Gang Du, Zhi-Hua Liu, Min Zhou, Guang-Yu Yang, Qiu-Fen Hu, Xue-Mei Li
Three new isopentylated diphenyl ethers, (1-3), together with two known isopentylated diphenyl ethers derivatives (4 and 5) were isolated from the fermentation products of an endophytic fungus Phomopsis fukushii. Their structures were elucidated by spectroscopic methods, including extensive 1D- and 2D NMR techniques. Compounds 1-3 were evaluated for their anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activity. The results showed that compounds 1-3 showed strong activity with diameter of inhibition zone (IZD) of 21...
January 18, 2018: Journal of Antibiotics
https://www.readbyqxmd.com/read/29348510/genome-and-secretome-analysis-of-pochonia-chlamydosporia-provide-new-insight-into-egg-parasitic-mechanisms
#4
Runmao Lin, Feifei Qin, Baoming Shen, Qianqian Shi, Chichuan Liu, Xi Zhang, Yang Jiao, Jun Lu, Yaoyao Gao, Marta Suarez-Fernandez, Federico Lopez-Moya, Luis Vicente Lopez-Llorca, Gang Wang, Zhenchuan Mao, Jian Ling, Yuhong Yang, Xinyue Cheng, Bingyan Xie
Pochonia chlamydosporia infects eggs and females of economically important plant-parasitic nematodes. The fungal isolates parasitizing different nematodes are genetically distinct. To understand their intraspecific genetic differentiation, parasitic mechanisms, and adaptive evolution, we assembled seven putative chromosomes of P. chlamydosporia strain 170 isolated from root-knot nematode eggs (~44 Mb, including 7.19% of transposable elements) and compared them with the genome of the strain 123 (~41 Mb) isolated from cereal cyst nematode...
January 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29339655/the-impact-of-arterial-clamping-technique-in-robot-assisted-partial-nephrectomy-on-renal-function-and-surgical-outcomes-six-year-experience-at-siriraj-hospital-thailand
#5
Tawatchai Taweemonkongsap, Chalairat Suk-Ouichai, Ekkarin Chotikawanich, Siros Jitpraphai, Varat Woranisarakul, Patkawat Ramart, Kittipong Phinthusophon, Teerapon Amornvesukit, Sunai Leewansangtong, Sittiporn Srinualnad, Chaiyong Nualyong
INTRODUCTION: Robot-assisted partial nephrectomy (RAPN) with different arterial clamping techniques has increasingly been performed to avoid ischemic injury to nephron. However, postoperative renal function remains controversial. We determine the impact of each renal arterial clamping on surgical and renal outcomes after RAPN. MATERIALS AND METHODS: Patients who underwent RAPN at Siriraj Hospital from 2010 to 2016 were retrospectively reviewed and stratified into 3 cohorts: main-clamp (MAC), selective-clamp, and off-clamp...
January 16, 2018: Urologia Internationalis
https://www.readbyqxmd.com/read/29338435/three-new-diphenyl-ether-derivatives-from-the-fermentation-products-of-an-endophytic-fungus-phomopsis-fukushii
#6
Yu-Hong Gao, Rui Zheng, Jing Li, Wei-Song Kong, Xin Liu, Lin Ye, Qi-Li Mi, Wei-Song Kong, Min Zhou, Guang-Yu Yang, Qiu-Fen Hu, Gang Du, Hai-Ying Yang, Xue-Mei Li
Three new diphenyl ethers (1-3), together with four known isopentylated diphenyl ethers derivatives (4-7), were isolated from the fermentation products of an endophytic fungus Phomopsis fukushii. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. Compounds 1-3 were evaluated for their anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activity. The results revealed that compounds 1 and 2 showed strong inhibitions with inhibition zone diameters (IZD) of 20...
January 17, 2018: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/29337927/the-plant-growth-promoting-fungus-pgpf-alternaria-sp-a13-markedly-enhances-salvia-miltiorrhiza-root-growth-and-active-ingredient-accumulation-under-greenhouse-and-field-conditions
#7
Li Si Zhou, Kun Tang, Shun Xing Guo
Plant growth-promoting fungi (PGPF) have attracted considerable interest as bio-fertilisers due to their multiple beneficial effects on plant quantity and quality and their positive relationship with the ecological environment. Advancements in the development of PGPF for crops and economic plant cultivation applications have been achieved, but such improvements for the use of PGPF with popular medicinal herbs, such as Salvia miltiorrhiza, are rare. In this study, we collected S. miltiorrhiza specimens inhabiting wild, semi-wild, farmland and pot-cultured areas in the Henan province of China and isolated endophytes from the roots, shoots and leaves of these samples...
January 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29335919/inter-and-intracellular-colonization-of-arabidopsis-roots-by-endophytic-actinobacteria-and-the-impact-of-plant-hormones-on-their-antimicrobial-activity
#8
Anne van der Meij, Joost Willemse, Martinus A Schneijderberg, René Geurts, Jos M Raaijmakers, Gilles P van Wezel
Many actinobacteria live in close association with eukaryotes such as fungi, insects, animals and plants. Plant-associated actinobacteria display (endo)symbiotic, saprophytic or pathogenic life styles, and can make up a substantial part of the endophytic community. Here, we characterised endophytic actinobacteria isolated from root tissue of Arabidopsis thaliana (Arabidopsis) plants grown in soil from a natural ecosystem. Many of these actinobacteria belong to the family of Streptomycetaceae with Streptomyces olivochromogenes and Streptomyces clavifer as well represented species...
January 15, 2018: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29335775/a-new-genus-planticonsortium-mucoromycotina-and-new-combination-p-tenue-for-the-fine-root-endophyte-glomus-tenue-basionym-rhizophagus-tenuis
#9
Christopher Walker, Armelle Gollotte, Dirk Redecker
In 1977, the fine root endophyte, originally named Rhizophagus tenuis, was transferred into the genus Glomus as G. tenue, thus positioning the species with all other known arbuscular mycorrhizal fungi (Glomeromycota, Glomeromycotina). Recent molecular evidence, however, places it in a different subphylum, Mucoromycotina in the Mucoromycota. No suitable genus exists in the Mucoromycotina to accommodate G. tenue, so it is moved to Planticonsortium gen. nov. as P. tenue comb. nov.
January 15, 2018: Mycorrhiza
https://www.readbyqxmd.com/read/29335638/clearcutting-alters-decomposition-processes-and-initiates-complex-restructuring-of-fungal-communities-in-soil-and-tree-roots
#10
Petr Kohout, Markéta Charvátová, Martina Štursová, Tereza Mašínová, Michal Tomšovský, Petr Baldrian
Forest management practices often severely affect forest ecosystem functioning. Tree removal by clearcutting is one such practice, producing severe impacts due to the total reduction of primary productivity. Here, we assessed changes to fungal community structure and decomposition activity in the soil, roots and rhizosphere of a Picea abies stand for a 2-year period following clearcutting compared to data from before tree harvest. We found that the termination of photosynthate flow through tree roots into soil is associated with profound changes in soil, both in decomposition processes and fungal community composition...
January 15, 2018: ISME Journal
https://www.readbyqxmd.com/read/29334729/polyoxygenated-cyclohexenoids-with-promising-%C3%AE-glycosidase-inhibitory-activity-produced-by-phomopsis-sp-ye3250-an-endophytic-fungus-derived-from-paeonia-delavayi
#11
Rong Huang, Bo-Guang Jiang, Xiao-Nian Li, Ya-Ting Wang, Si-Si Liu, Kai-Xuan Zheng, Jian He, Shao-Hua Wu
Seven new polyoxygenated cyclohexenoids, namely, phomopoxides A-G (1-7), were isolated from the fermentation broth extract of an endophytic fungal strain Phomopsis sp. YE3250 from the medicinal plant Paeonia delavayi Franch. The structures of these compounds were established by spectroscopic interpretation. The absolute configurations of compounds 1 and 4 were confirmed by X-ray crystallographic analysis and chemical derivative approach. All isolated compounds showed weak cytotoxic activities towards three human tumor cell lines (Hela, MCF-7, and NCI-H460), and weak antifungal activities against five pathogenic fungi (Candida albicans, Aspergillus niger, Pyricularia oryzae, Fusarium avenaceum, and Hormodendrum compactum)...
January 15, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29334248/nigrosporanenes-c-and-d-two-new-cyclohexene-derivatives-from-the-enphytic-fungus-nigrospora-oryzae-s4
#12
Wei Yan, Wei Wei, Yang-Yang Zhang, Jia-Jie Wang, Xiao-Fei Ping, Jia-Yun Xu, Yong-Hao Ye
Two new cyclohexene derivatives, nigrosporanenes C and D (1 and 2), together with three known compounds (3-5), were isolated from the culture of an endophyte Nigrospora oryzae S4. Their structures were characterized by a combination of detailed spectroscopic analysis and comparison of their NMR data with those reported in the literature. All compounds were tested for anti-phytopathogenic activity, however, none of them showed activity at a concentration of 20 μM.
January 15, 2018: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/29332277/efficient-degradation-of-triclosan-by-an-endophytic-fungus-penicillium-oxalicum-b4
#13
Hao Tian, Yan Jun Ma, Wan Yi Li, Jian Wen Wang
Triclosan (TCS), a widely used antimicrobial and preservative agent, is an emerging contaminant in aqueous and soil environment. Microbial degradation of TCS has not been reported frequently because of its inhibition of microbe growth. To explore the new microbial resources for TCS biodegradation, fungal endophytes were isolated and screened for the degradation potential. The endophytic strain B4 isolated from Artemisia annua L. showed higher degradation efficiency and was identified as Penicillium oxalicum based on its morphology and ITS sequences of ribosomal DNA...
January 13, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29330819/preparation-of-a-synthetic-seed-for-the-common-reed-harboring-an-endophytic-bacterium-promoting-seedling-growth-under-cadmium-stress
#14
Ting Gao, Xianyang Shi
Bacterial seed endophytes can facilitate germination and early plant development. Therefore, the introduction of seed-borne endophytes may improve selected plant characteristics across generations. In this study, regenerated plantlets of common reed (Phragmites australis) were inoculated with activated sludge to obtain a specific functional endophytic bacterium. Denaturing gradient gel electrophoresis demonstrated that abundant endophytic bacteria could be enriched in the roots. A siderophore-producing endophytic bacterium was isolated from the roots and identified as Herbaspirillum frisingense RE3-3 based on 16S rRNA sequences...
January 12, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29330499/three-dimensional-physical-model-assisted-planning-and-navigation-for-laparoscopic-partial-nephrectomy-in-patients-with-endophytic-renal-tumors
#15
Gang Fan, Jun Li, Mingfeng Li, Mingji Ye, Xiaming Pei, Feiping Li, Shuai Zhu, Han Weiqin, Xiao Zhou, Yu Xie
Resection of completely endophytic renal tumors is a huge challenge for surgeons due to a lack of definite visual clues, especially in the laparoscopic approach. Three-dimensional (3D) kidney models, which can illustrate the clear relationship between renal masses and surrounding health tissues, were considered as reliable tools for understanding renal tumor characteristics in previous studies. We hypothesized that 3D kidney models can be used not only for planning but also for navigating laparoscopic partial nephrectomy (LPN) in patients with completely endophytic renal tumors...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29327464/the-expression-of-an-exogenous-acc-deaminase-by-the-endophyte-serratia-grimesii-bxf1-promotes-the-early-nodulation-and-growth-of-common-bean
#16
Maria J Tavares, Francisco X Nascimento, Bernard R Glick, Márcio J Rossi
Ethylene acts as an inhibitor of the nodulation process of leguminous plants. However, some bacteria can decrease deleterious ethylene levels by the action of the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase which degrades ACC, the ethylene precursor in all higher plants. Co-inoculation of rhizobia with endophytes enhances the rhizobial symbiotic efficiency with legumes, improving both nodulation and nitrogen fixation. However, not much is understood about the mechanisms employed by these endophytic bacteria...
January 11, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29327320/phylogenetic-analysis-and-biological-evaluation-of-marine-endophytic-fungi-derived-from-red-sea-sponge-hyrtios-erectus
#17
Mervat Morsy Abbas Ahmed El-Gendy, Shaymaa M M Yahya, Ahmed R Hamed, Maha M Soltan, Ahmed Mohamed Ahmed El-Bondkly
Forty-four endophytic fungal isolates obtained from marine sponge, Hyrtios erectus, were evaluated and screened for their hydrolase activities. Most of the isolates were found to be prolific producers of hydrolytic enzymes. Only 11 isolates exhibited maximum cellular contents of lipids, rhamnolipids, and protein in the fungal isolates under the isolation numbers MERVA5, MERVA22, MERVA25, MERVA29, MERVA32, MERVA34, MERV36, MERVA39, MERVA42, MERVA43, and MERVA44. These isolate extracts exhibit the highest reducing activities against carbohydrate-metabolizing enzymes including α-amylase, α-glucosidase, β-glucosidase, β-glucuronidase, and tyrosinase...
January 12, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29327048/retinoblastoma-with-endophytic-and-exophytic-features
#18
Manuel Paez-Escamilla, Nika Bagheri, J William Harbour
No abstract text is available yet for this article.
January 11, 2018: JAMA Ophthalmology
https://www.readbyqxmd.com/read/29325988/arboriscoccus-pini-gen-nov-sp-nov-an-endophyte-from-a-pine-tree-of-the-class-alphaproteobacteria-emended-description-of-geminicoccus-roseus-and-proposal-of-geminicoccaceae-fam-nov
#19
Diogo N Proença, William B Whitman, Neha Varghese, Nicole Shapiro, Tanja Woyke, Nikos C Kyrpides, Paula V Morais
Bacterial strain B29T1T was isolated from the endophytic microbial community of a Pinus pinaster tree trunk and characterized. Strain B29T1T stained Gram-negative and formed diplococci that grew optimally at 26-30°C and at pH 6.0-7.0. The G+C content of the DNA was 61.6mol%. The respiratory quinone was ubiquinone 10 (UK-10), and the major fatty acids were C16:0, cyclo-C19:0ω8c and C18:0 12-methyl, representing 64% of the total fatty acids. Phylogenetic analyses based on the 16S rRNA gene sequences placed strain B29T1T within the order Rhodospirillales in a distinct lineage that also included the genus Geminicoccus...
December 22, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/29320724/purification-and-characterization-of-a-novel-high-molecular-weight-alkaline-protease-produced-by-an-endophytic-bacillus-halotolerans-strain-ct2
#20
Gharbi Dorra, Karkouch Ines, Ben Slimene Imen, Coquet Laurent, Azaiez Sana, Olfa Tabbene, Cosette Pascal, Jouenne Thierry, Limam Ferid
A protease-producing strain CT2 isolated from Tunisian potatoes, exhibiting a potent protease activity (prot CT2), was identified as Bacillus halotolerans according to 16S ribosomal DNA sequence analysis. Maximum prot CT2 production was obtained in medium supplemented with bean seed proteins. Proteolytic activity was purified by ammonium sulphate precipitation, Sephacryl S-200 gel filtration and SP-sepharose cation-exchange chromatography. Optimal enzyme activity was reached at pH 9 and temperature of 50 °C...
January 7, 2018: International Journal of Biological Macromolecules
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