Karina A Serban, Samin Rezania, Daniela N Petrusca, Christophe Poirier, Danting Cao, Matthew J Justice, Milan Patel, Irina Tsvetkova, Krzysztof Kamocki, Andrew Mikosz, Kelly S Schweitzer, Sean Jacobson, Angelo Cardoso, Nadia Carlesso, Walter C Hubbard, Katerina Kechris, Bogdan Dragnea, Evgeny V Berdyshev, Jeanette McClintock, Irina Petrache
Circulating endothelial microparticles (EMPs) are emerging as biomarkers of chronic obstructive pulmonary disease (COPD) in individuals exposed to cigarette smoke (CS), but their mechanism of release and function remain unknown. We assessed biochemical and functional characteristics of EMPs and circulating microparticles (cMPs) released by CS. CS exposure was sufficient to increase microparticle levels in plasma of humans and mice, and in supernatants of primary human lung microvascular endothelial cells. CS-released EMPs contained predominantly exosomes that were significantly enriched in let-7d, miR-191; miR-126; and miR125a, microRNAs that reciprocally decreased intracellular in CS-exposed endothelium...
August 17, 2016: Scientific Reports