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Yuting Qin, Lingling Wu, Ye Ouyang, Ping Zhou, Haibo Zhou, Yan Wang, Jianyang Ma, Jinsong Zhang, Yanan Chen, Jie Qian, Yuanjia Tang, Nan Shen
MicroRNAs are universal post-transcriptional regulators in genomes. They have the ability of buffering gene expressional programs, contributing to robustness of biological systems and playing important roles in development, physiology and diseases. Here, we identified a microRNA, miR-125a, as a positive regulator of granulopoiesis. MiR125a knockout mice show reduced infiltration of neutrophils in the lung and alleviated tissue destruction after endotoxin challenge as a consequence of decreased neutrophil numbers...
October 2017: PLoS Genetics
Giorgio Aquila, Cinzia Fortini, Antonio Pannuti, Serena Delbue, Micaela Pannella, Marco Bruno Morelli, Cristiana Caliceti, Fausto Castriota, Monica de Mattei, Alessia Ongaro, Agnese Pellati, Pasquale Ferrante, Lucio Miele, Luigi Tavazzi, Roberto Ferrari, Paola Rizzo, Alberto Cremonesi
BACKGROUND: The lack of early diagnosis, progression markers and effective pharmacological treatment has dramatic unfavourable effects on clinical outcomes in patients with peripheral artery disease (PAD). Addressing these issues will require dissecting the molecular mechanisms underlying this disease. We sought to characterize the Notch signaling and atherosclerosis relevant markers in lesions from femoral arteries of symptomatic PAD patients. METHODS: Plaque material from the common femoral, superficial femoral or popliteal arteries of 20 patients was removed by directional atherectomy...
May 4, 2017: Journal of Translational Medicine
TianTian Cai, Jie Li, Xiaofei An, Ni Yan, Danfeng Li, Yanfei Jiang, Wen Wang, Liangfeng Shi, Qiu Qin, Ronghua Song, Guofei Wang, Wenjuan Jiang, Jin-An Zhang
BACKGROUND: Single nucleotide polymorphisms (SNPs) of the miR-146a, miR-499a and miR-125a have been shown to be associated with the susceptibility to several autoimmune diseases. This study was conducted to identify the association of SNPs rs2910164, rs57095329, rs3746444 and rs12976445 with autoimmune thyroid diseases (AITDs) in a Chinese Han population. METHODS: We enrolled 1061 patients with AITDs, including 701 patients with Graves' disease (GD) and 360 patients with Hashimoto's thyroiditis (HT), and 938 healthy individuals for a case-control genetic association study...
January 15, 2017: Molecular and Cellular Endocrinology
Karina A Serban, Samin Rezania, Daniela N Petrusca, Christophe Poirier, Danting Cao, Matthew J Justice, Milan Patel, Irina Tsvetkova, Krzysztof Kamocki, Andrew Mikosz, Kelly S Schweitzer, Sean Jacobson, Angelo Cardoso, Nadia Carlesso, Walter C Hubbard, Katerina Kechris, Bogdan Dragnea, Evgeny V Berdyshev, Jeanette McClintock, Irina Petrache
Circulating endothelial microparticles (EMPs) are emerging as biomarkers of chronic obstructive pulmonary disease (COPD) in individuals exposed to cigarette smoke (CS), but their mechanism of release and function remain unknown. We assessed biochemical and functional characteristics of EMPs and circulating microparticles (cMPs) released by CS. CS exposure was sufficient to increase microparticle levels in plasma of humans and mice, and in supernatants of primary human lung microvascular endothelial cells. CS-released EMPs contained predominantly exosomes that were significantly enriched in let-7d, miR-191; miR-126; and miR125a, microRNAs that reciprocally decreased intracellular in CS-exposed endothelium...
August 17, 2016: Scientific Reports
Vahid Khori, Ali Mohammad Alizadeh, Zohre Gheisary, Sadaf Farsinejad, Farrokh Najafi, Solmaz Khalighfard, Fatemeh Ghafari, Maryam Hadji, Hamid Khodayari
Low-level laser therapy (LLLT) is a form of photon therapy which can be a non-invasive therapeutic procedure in cancer therapy using low-intensity light in the range of 450-800 nm. One of the main functional features of laser therapy is the photobiostimulation effects of low-level lasers on various biological systems including altering DNA synthesis and modifying gene expression, and stopping cellular proliferation. This study investigated the effects of LLLT on mice mammary tumor and the expression of Let-7a, miR155, miR21, miR125, and miR376b in the plasma and tumor samples...
December 2016: Lasers in Medical Science
Mehmet Akif Camkurt, Fatih Karababa, Mehmet Emin Erdal, Hüseyin Bayazıt, Sultan Basmacı Kandemir, Mustafa Ertan Ay, Hasan Kandemir, Özlem İzci Ay, Erdinç Çiçek, Salih Selek, Bahar Taşdelen
OBJECTIVE: The prevalence of schizophrenia is 1%, and it is a debilitating disorder that often results in a shortened lifespan. Peripheral blood samples are good candidates to investigate because they can be easily drawn, and they are widely studied in psychiatric disorders. MicroRNAs are small non-coding RNA transcripts. They regulate the expression of genes by binding to the 3'-untranslated region (UTR) of mRNAs and pointing them to degrade. In this study, we aimed to investigate the expression of miR-9-5p, miR-29a-3p, miR-106-5p, miR-106b-5p, miR-107, miR-125a-3p, and miR-125b-3p in schizophrenia patients and healthy controls...
August 31, 2016: Clinical Psychopharmacology and Neuroscience: the Official Scientific Journal of the Korean College of Neuropsychopharmacology
Clark C Chen, Patryk Moskwa, Pascal O Zinn, Brian R Hirshman, Young Eun Choi, Sachet A Shukla, Wojciech Fendler, Jun Lu, Todd R Golub, Anita Hjelmeland, Dipanjan Chowdhury
INTRODUCTION: The efficacy of radiotherapy in many tumor types is limited by normal tissue toxicity and by intrinsic or acquired radioresistance. METHODS: An unbiased functional microRNA screen identified 4 miRNAs (miR1, miR125a, miR150, and miR425) that induced glioblastoma radioresistance. We employed gain and loss of function approaches to validate the critical importance of these miRNAs as determinants of glioblastoma radiation resistance. RESULTS: Overexpression of miR1, miR125a, miR150, and/or miR425 in glioblastoma promotes radioresistance through upregulation of the cell-cycle checkpoint response...
August 2016: Neurosurgery
Yunha Kim, Junghee Lee, Hoon Ryu
MicroRNAs (miRNAs) can regulate the expression of genes that are involved in multiple cellular pathways. However, their targets and mechanism of action associated with the autophagy pathway are not fully investigated yet. EWSR1 (EWS RNA-Binding Protein 1/Ewing Sarcoma Break Point Region 1) gene encodes a RNA/DNA binding protein that is ubiquitously expressed and plays roles in numerous cellular processes. Recently, our group has shown that EWSR1 deficiency leads to developmental failure and accelerated senescence via processing of miRNAs, but its role in the regulation of autophagy remains elusive...
July 2015: BMB Reports
Marius F Ifrim, Kathryn R Williams, Gary J Bassell
Fragile X syndrome (FXS) is caused by the loss of the fragile X mental retardation protein (FMRP), an RNA binding protein that regulates translation of numerous target mRNAs, some of which are dendritically localized. Our previous biochemical studies using synaptoneurosomes demonstrate a role for FMRP and miR-125a in regulating the translation of PSD-95 mRNA. However, the local translation of PSD-95 mRNA within dendrites and spines, as well as the roles of FMRP or miR-125a, have not been directly studied. Herein, local synthesis of a Venus-PSD-95 fusion protein was directly visualized in dendrites and spines using single-molecule imaging of a diffusion-restricted Venus-PSD-95 reporter under control of the PSD-95 3'UTR...
May 6, 2015: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
Yunha Kim, Young-Sook Kang, Na-Young Lee, Ki Yoon Kim, Yu Jin Hwang, Hyun-Wook Kim, Im Joo Rhyu, Song Her, Min-Kyung Jung, Sun Kim, Chai-Jin Lee, Seyoon Ko, Neil W Kowall, Sean Bong Lee, Junghee Lee, Hoon Ryu
The EWSR1 (EWS RNA-binding protein 1/Ewing Sarcoma Break Point Region 1) gene encodes a RNA/DNA binding protein that is ubiquitously expressed and involved in various cellular processes. EWSR1 deficiency leads to impairment of development and accelerated senescence but the mechanism is not known. Herein, we found that EWSR1 modulates the Uvrag (UV radiation resistance associated) gene at the post-transcription level. Interestingly, EWSR1 deficiency led to the activation of the DROSHA-mediated microprocessor complex and increased the level of Mir125a and Mir351, which directly target Uvrag...
2015: Autophagy
Perrine J Martin, Nathalie Haren, Olfa Ghali, Aline Clabaut, Christophe Chauveau, Pierre Hardouin, Odile Broux
BACKGROUND: In osteoporosis, bone loss is accompanied by increased marrow adiposity. Given their proximity in the bone marrow and their shared origin, a dialogue between adipocytes and osteoblasts could be a factor in the competition between human Mesenchymal Stem Cells (hMSC) differentiation routes, leading to adipocyte differentiation at the expense of osteoblast differentiation. The adipocyte/osteoblast balance is highly regulated at the level of gene transcription. In our work, we focused on PPARgamma, CEBPalpha and CEBPdelta, as these transcription factors are seen as master regulators of adipogenesis and expressed precociously, and on leptin and adiponectin, considered as adipocyte marker genes...
2015: BMC Cell Biology
Christopher S Inchley, Tonje Sonerud, Hans O Fjærli, Britt Nakstad
BACKGROUND: Respiratory syncytial virus (RSV) infection is a common cause of pediatric hospitalization. microRNA, key regulators of the immune system, have not previously been investigated in respiratory specimens during viral infection. We investigated microRNA expression in the nasal mucosa of 42 RSV-positive infants, also comparing microRNA expression between disease severity subgroups. METHODS: Nasal mucosa cytology specimens were collected from RSV-positive infants and healthy controls...
2015: BMC Infectious Diseases
Jianjian Zheng, Zhenxu Zhou, Ziqiang Xu, Guojun Li, Peihong Dong, Zhanguo Chen, Dezhao Lin, Bicheng Chen, Fujun Yu
It has been demonstrated that liver microRNA-125a-5p (miR-125a-5p) is correlated with disease progression in different liver diseases, including liver fibrosis and hepatocellular carcinoma (HCC). The present study investigated whether serum miR-125a-5p correlated with the progression of different liver diseases. Serum samples were obtained from healthy individuals, patients with chronic hepatitis B who had undergone a liver biopsy, and patients with HCC and were analyzed for the levels of miR-125a-5p. Compared with the healthy controls, the serum levels of miR-125a-5p were significantly higher in the liver fibrosis serum, and were reduced in HCC...
July 2015: Molecular Medicine Reports
Lei Sun, Baogang Zhang, Yuqing Liu, Lihong Shi, Hongli Li, Shijun Lu
Poor prognosis of glioma is due to the characteristics of high invasiveness. Recently, it was demonstrated that Gab2 was over-expressed and related to cellular migration and invasion in glioma, however, the mechanisms of regulation are still unknown. A better understanding of molecular events key to the carcinogenesis and tumor progression may facilitate development of new therapeutic targets and anti-glioma strategies. This study is the first to focus on miR125a-5p, which was predicted to regulate Gab2 with directly targeting the 3' un-translated region (3'UTR) of Gab2 and could inhibit migration and invasion of glioma cells by mediating Gab2 to affect cytoskeleton rearrangement and matrix metalloproteinases expression...
January 2016: Molecular Carcinogenesis
Xuehu Xu, Xiaobing Wu, Yong Li, Haibo Liu, Qinping Jiang, Yan Sun
OBJECTIVE: To examine the differential expression of miRNAs in colon cancer tissues and matched tumor adjacent tissues. METHODS: Differential expression of microRNAs in twenty paired human colon cancer tissues and adjacent tissues were detected by QPCR. miRNAs with significantly differential expression (fold change >2.4 and P<0.01) were screened to analyze their accumulation by Cluster analysis. Thus correlation of miRNA and other colon cancer-associated proteins was examined...
November 2014: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
Patryk Moskwa, Pascal O Zinn, Young Eun Choi, Sachet A Shukla, Wojciech Fendler, Clark C Chen, Jun Lu, Todd R Golub, Anita Hjelmeland, Dipanjan Chowdhury
UNLABELLED: The efficacy of radiotherapy in many tumor types is limited by normal tissue toxicity and by intrinsic or acquired radioresistance. Therefore, it is essential to understand the molecular network responsible for regulating radiosensitivity/resistance. Here, an unbiased functional screen identified four microRNAs (miR1, miR125a, miR150, and miR425) that induce radioresistance. Considering the clinical importance of radiotherapy for patients with glioblastoma, the impact of these miRNAs on glioblastoma radioresistance was investigated...
December 2014: Molecular Cancer Research: MCR
Y Inoue, M Watanabe, N Inoue, T Kagawa, S Shibutani, H Otsu, M Saeki, Y Takuse, Y Hidaka, Y Iwatani
It is important to search the biomarker to predict the development and prognosis of autoimmune thyroid diseases (AITDs) such as Hashimoto's disease (HD) and Graves' disease (GD). MicroRNA (miR) bind directly to the 3' untranslated region of specific target mRNAs to suppress the expression of proteins, promote the degradation of target mRNAs and regulate immune response. miR-125a is known to be a negative regulator of regulated upon activation normal T cell expressed and secreted (RANTES), interleukin (IL)-6 and transforming growth factor (TGF)-β; however, its association with AITDs remains unknown...
November 2014: Clinical and Experimental Immunology
Elvira L Liclican, Tonya C Walser, Saswati Hazra, Kostyantyn Krysan, Stacy J Park, Paul C Pagano, Brian K Gardner, Jill E Larsen, John D Minna, Steven M Dubinett
Understanding the molecular pathogenesis of lung cancer is necessary to identify biomarkers/targets specific to individual airway molecular profiles and to identify options for targeted chemoprevention. Herein, we identify mechanisms by which loss of microRNA (miRNA)125a-3p (miR125a) contributes to the malignant potential of human bronchial epithelial cells (HBEC) harboring an activating point mutation of the K-ras proto-oncogene (HBEC K-ras). Among other miRNAs, we identified significant miR125a loss in HBEC K-ras lines and determined that miR125a is regulated by the PEA3 transcription factor...
August 2014: Cancer Prevention Research
Xinhuan Zhang, Shanshan Shao, Houfa Geng, Yong Yu, Chenggang Wang, Zhanfeng Liu, Chunxiao Yu, Xiuyun Jiang, Yangxin Deng, Ling Gao, Jiajun Zhao
CONTEXT: Increasing evidence shows that subclinical hypothyroidism (SCH) is associated with atherosclerosis (ATH), but the association remains controversial. MicroRNAs (miRNAs) have been proved to be involved in atherosclerosis and dyslipidemia as gene regulators. OBJECTIVE: The objective of the study was to determine the expression profiles of six serum miRNAs critical to atherosclerosis in SCH patients and reanalyze the association between atherosclerosis and SCH from a new perspective...
May 2014: Journal of Clinical Endocrinology and Metabolism
Gian Matteo Rigolin, Elena Saccenti, Lara Rizzotto, Manuela Ferracin, Sara Martinelli, Luca Formigaro, Francesca Cibien, Maurizio Cavallari, Enrico Lista, Giulia Daghia, Olga Sofritti, Maria Ciccone, Francesco Cavazzini, Laura Lupini, Cristian Bassi, Barbara Zagatti, Massimo Negrini, Antonio Cuneo
The majority of patients with chronic lymphocytic leukemia (CLL) and favorable prognostic features live for long periods without treatment. However, unexpected disease progression is observed in some cases. In a cohort of untreated CD38- CLL patients with normal FISH or isolated 13q- we found that, by fluorescence in situ hybridization (FISH), 16/28 cases presented, within immunomagnetic sorted CD38+ cells, genetic lesions undetectable in the CD38- fraction. These patients showed a shorter time to first treatment (TTFT, p=0...
January 15, 2014: Oncotarget
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