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Protein membrane interactions

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https://www.readbyqxmd.com/read/28742972/stimuli-responsive-directional-vesicular-assembly-with-tunable-surface-functionality-and-impact-on-enzyme-inhibition
#1
Amrita Sikder, Debes Ray, Vinod Kumar Aswal, Suhrit Ghosh
The article describes self-assembly of a series of unsymmetrical bola-shape π-amphiphiles (NDI-1, NDI-1a, NDI-2, NDI-3 and NDI-4) consisting of a hydrophobic naphthalene-diimide (NDI) chromophore attached with a non-ionic hydrophilic wedge and an anionic head group in the two opposite arms of the central NDI. By design only a single hydrazide group is linked either on the ionic or non-ionic arm of the NDI. NDI-1 and NDI-1a are regioisomers differing only in the location of the hydrazide group, placed in the non-ionic or ionic arm, respectively...
July 25, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28742866/contribution-of-factor-h-binding-protein-sequence-to-the-cross-reactivity-of-meningococcal-native-outer-membrane-vesicle-vaccines-with-over-expressed-fhbp-variant-group-1
#2
Arianna Marini, Omar Rossi, Maria Grazia Aruta, Francesca Micoli, Simona Rondini, Serafina Guadagnuolo, Isabel Delany, Ian R Henderson, Adam F Cunningham, Allan Saul, Calman A MacLennan, Oliver Koeberling
Factor H-binding protein (fHbp) is an important meningococcal vaccine antigen. Native outer membrane vesicles with over-expressed fHbp (NOMV OE fHbp) have been shown to induce antibodies with broader functional activity than recombinant fHbp (rfHbp). Improved understanding of this broad coverage would facilitate rational vaccine design. We performed a pair-wise analysis of 48 surface-exposed amino acids involved in interacting with factor H, among 383 fHbp variant group 1 sequences. We generated isogenic NOMV-producing meningococcal strains from an African serogroup W isolate, each over-expressing one of four fHbp variant group 1 sequences (ID 1, 5, 9, or 74), including those most common among invasive African meningococcal isolates...
2017: PloS One
https://www.readbyqxmd.com/read/28742858/reduction-of-endoplasmic-reticulum-mitochondria-interactions-in-beta-cells-from-patients-with-type-2-diabetes
#3
Charles Thivolet, Guillaume Vial, Romeo Cassel, Jennifer Rieusset, Anne-Marie Madec
Type 2 diabetes develops when beta cells are not able to fulfill insulin needs. The role of the endoplasmic reticulum-mitochondria junction in coordinating the functions of these two organelles throughout the natural history of type 2 diabetes is determinant and may explain the alterations of insulin biosynthesis. Our goal was to study endoplasmic reticulum and mitochondrial interactions in human beta cells from organ donors with type 2 diabetes. Pancreas samples were obtained via the network for pancreatic organ donors with diabetes (nPOD) based on disease status with 12 subjects with type 2 diabetes and 9 non-diabetic controls...
2017: PloS One
https://www.readbyqxmd.com/read/28742600/cholesterol-reduction-and-macrophage-function-role-of-paraoxonases
#4
C Roger White, G M Anantharamaiah
PURPOSE OF REVIEW: Unregulated uptake of oxidized LDL by macrophages to form foam cells is the hallmark for atherosclerosis. The paraoxonase (PON) family of enzymes plays a critical role in attenuating atherosclerotic lesion formation by hydrolyzing lipid peroxides (LOOHs) and preventing the oxidation of LDL particles and by enhancing HDL-mediated cholesterol efflux. Findings in recent years suggest novel mechanisms by which PON isoforms interact with macrophages to regulate cholesterol metabolism and cellular function...
July 22, 2017: Current Opinion in Lipidology
https://www.readbyqxmd.com/read/28742147/genetic-analysis-of-the-regulation-of-the-voltage-gated-calcium-channel-homolog-cch1-by-the-%C3%AE-subunit-homolog-ecm7-and-cortical-er-protein-scs2-in-yeast
#5
Takafumi Kato, Aya Kubo, Tatsuya Nagayama, Shinichiro Kume, Chikara Tanaka, Yoshitaka Nakayama, Kazuko Iida, Hidetoshi Iida
The yeast Cch1/Mid1 Ca2+ channel is equivalent to animal voltage-gated Ca2+ channels and activated in cells incubated in low Ca2+ medium. We herein investigated the third subunit, Ecm7, under the same cell culture conditions. The deletion of ECM7 slightly lowered Ca2+ influx activity in the CNB1+ background, in which calcineurin potentially dephosphorylates Cch1, but markedly lowered this activity in the cnb1Δ background. The deletion of the C-terminal cytoplasmic region of Ecm7 also reduced Ca2+ influx activity...
2017: PloS One
https://www.readbyqxmd.com/read/28742068/a-peptide-based-viral-inactivator-inhibits-zika-virus-infection-in-pregnant-mice-and-fetuses
#6
Yufeng Yu, Yong-Qiang Deng, Peng Zou, Qian Wang, Yanyan Dai, Fei Yu, Lanying Du, Na-Na Zhang, Min Tian, Jia-Nan Hao, Yu Meng, Yuan Li, Xiaohui Zhou, Jasper Fuk-Woo Chan, Kwok-Yung Yuen, Cheng-Feng Qin, Shibo Jiang, Lu Lu
Zika virus (ZIKV), a re-emerging flavivirus associated with neurological disorders, has spread rapidly to more than 70 countries and territories. However, no specific vaccines or antiviral drugs are currently available to prevent or treat ZIKV infection. Here we report that a synthetic peptide derived from the stem region of ZIKV envelope protein, designated Z2, potently inhibits infection of ZIKV and other flaviviruses in vitro. We show that Z2 interacts with ZIKV surface protein and disrupts the integrity of the viral membrane...
July 25, 2017: Nature Communications
https://www.readbyqxmd.com/read/28741693/antioxidants-protect-proteins-anchorage-to-the-bilayer-by-improving-plasma-membrane-integrity-of-ram-spermatozoa-during-liquid-preservation-in-a-soya-lecithin-based-diluent
#7
R K Paul, D Kumar, Smk Naqvi
Antioxidants are known to prevent the reactive oxygen species (ROS)-mediated peroxidative damage to the membrane lipids during hypothermic storage of mammalian spermatozoa. We hypothesized here that ROS also affect the lipid-protein interactions, thereby diminishing the membrane's integrity and proteins' anchorage to the bilayer. Antioxidants prevent these damages by scavenging the ROS. Ejaculates from Patanwadi rams were pooled after subjective evaluation and centrifuged using Percoll(®) . Sperm pellet was resuspended in soya lecithin-Tris-fructose diluent (400 × 10(6)  cells/ml) containing either antioxidants (100 IU/ml catalase + 10 mM reduced glutathione) or no antioxidant...
July 25, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28741466/binding-of-cxcr4-transmembrane-peptides-to-the-bacterial-chaperonin-groel
#8
Xiaoqiang Wang, Haixia Chi, Shixin Li, Yan Xu
: Newly translated membrane protein CXCR4 was observed to gain significant folding enhancement upon interacting with the bacterial chaperonin GroEL-GroES. The main fold of CXCR4 consists of 7 transmembrane α-helices distinct from soluble proteins that have dominated research on the chaperonin-assisted folding. This work extends our previous observation to the binding of GroEL with CXCR4 transmembrane peptides, which are mainly composed of hydrophobic residues characteristic of the substrates recognized by the chaperonin...
July 24, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28741036/network-based-subcellular-proteomics-in-monocyte-membrane-revealed-novel-candidate-genes-involved-in-osteoporosis
#9
Y Zeng, L Zhang, W Zhu, H He, H Sheng, Q Tian, F-Y Deng, L-S Zhang, H-G Hu, H-W Deng
In this study, label-free-based quantitative subcellular proteomics integrated with network analysis highlighted several candidate genes including P4HB, ITGB1, CD36, and ACTN1 that may be involved in osteoporosis. All of them are predicted as significant membrane proteins with high confidence and enriched in bone-related biological process. The results were further verified in transcriptomic and genomic levels. INTRODUCTION: Osteoporosis is a metabolic bone disease mainly characterized by low bone mineral density (BMD)...
July 24, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/28738898/silicanin-1-is-a-conserved-diatom-membrane-protein-involved-in-silica-biomineralization
#10
Alexander Kotzsch, Philip Gröger, Damian Pawolski, Paul H H Bomans, Nico A J M Sommerdijk, Michael Schlierf, Nils Kröger
BACKGROUND: Biological mineral formation (biomineralization) proceeds in specialized compartments often bounded by a lipid bilayer membrane. Currently, the role of membranes in biomineralization is hardly understood. RESULTS: Investigating biomineralization of SiO2 (silica) in diatoms we identified Silicanin-1 (Sin1) as a conserved diatom membrane protein present in silica deposition vesicles (SDVs) of Thalassiosira pseudonana. Fluorescence microscopy of GFP-tagged Sin1 enabled, for the first time, to follow the intracellular locations of a biomineralization protein during silica biogenesis in vivo...
July 24, 2017: BMC Biology
https://www.readbyqxmd.com/read/28738245/two-amino-acids-change-in-the-nsp4-of-sars-coronavirus-abolishes-viral-replication
#11
Yusuke Sakai, Kengo Kawachi, Yutaka Terada, Hiroko Omori, Yoshiharu Matsuura, Wataru Kamitani
Infection with coronavirus rearranges the host cell membrane to assemble a replication/transcription complex in which replication of the viral genome and transcription of viral mRNA occur. Although coexistence of nsp3 and nsp4 is known to cause membrane rearrangement, the mechanisms underlying the interaction of these two proteins remain unclear. We demonstrated that binding of nsp4 with nsp3 is essential for membrane rearrangement and identified amino acid residues in nsp4 responsible for the interaction with nsp3...
July 21, 2017: Virology
https://www.readbyqxmd.com/read/28738206/a-preliminary-study-on-the-effects-of-lanthanum-iii-on-plant-vitronectin-like-protein-and-its-toxicological-basis
#12
Lihong Wang, Jingfang He, Qing Yang, Xiaodong Li, Haiyan Wei, David D Y Chen, Xiaohua Huang
Vitronectin-like protein (VN) is widely found outside plant plasma membranes. The VN molecular surface contains a large number of active groups that combine strongly with rare earth elements (REEs), which means that VN is a preferential binding target for REEs exhibiting their toxic effects, but the toxicological mechanism remains unknown. This study used transmission electron microscopy, circular dichroism, fluorescence spectrometry, ultraviolet-visible spectroscopy, X-ray photoelectron spectroscopy, and calculational chemistry (homology modeling, molecular dynamics simulation and quantum chemical calculation) to preliminarily investigate the effect of lanthanum [La(III)] as an REE, on the structure of VN and its toxicological mechanism...
July 21, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28736556/ras-signaling-inhibitors-attenuate-disease-in-adjuvant-induced-arthritis-via-targeting-pathogenic-antigen-specific-th17-type-cells
#13
Morad Zayoud, Victoria Marcu-Malina, Einav Vax, Jasmine Jacob-Hirsch, Galit Elad-Sfadia, Iris Barshack, Yoel Kloog, Itamar Goldstein
The Ras family of GTPases plays an important role in signaling nodes downstream to T cell receptor and CD28 activation, potentially lowering the threshold for T-cell receptor activation by autoantigens. Somatic mutation in NRAS or KRAS may cause a rare autoimmune disorder coupled with abnormal expansion of lymphocytes. T cells from rheumatoid arthritis (RA) patients show excessive activation of Ras/MEK/ERK pathway. The small molecule farnesylthiosalicylic acid (FTS) interferes with the interaction between Ras GTPases and their prenyl-binding chaperones to inhibit proper plasma membrane localization...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28735865/rps27a-enhances-ebv-encoded-lmp1-mediated-proliferation-and-invasion-by-stabilizing-of-lmp1
#14
Seung-Woo Hong, Seung-Mi Kim, Dong-Hoon Jin, Yeong Seok Kim, Dae Young Hur
Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) is an oncoviral protein that plays a pivotal role in EBV-induced oncogenic transformation. The function of LMP1 in EBV-induced oncogenesis has been well studied. However, the molecular mechanisms underlying LMP1 protein stability remain poorly understood. In this study, we found that ribosomal protein s27a (RPS27a) regulates LMP1 stability by a tandem affinity purification analysis. RPS27a interacts directly with LMP1 in vitro and in vivo. Furthermore, overexpression of RPS27a increases the half-life of LMP1 in 293T cells, whereas downregulation of RPS27a using lentiviral shRNA technology accelerates the decrease in LMP1 protein level in EBV-transformed B cells...
July 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28735341/the-effect-of-nanosecond-high-voltage-electric-pulses-on-the-shape-and-permeability-of-polymersome-guvs
#15
Tina Batista Napotnik, Gianluca Bello, Eva-Kathrin Sinner, Damijan Miklavčič
Polymersomes, vesicles composed of block copolymers, are promising candidates as membrane alternatives and functional containers, e.g., as potential carriers for functional molecules because of their stability and tunable membrane properties. In the scope of possible use for membrane protein delivery to cells by electrofusion, we investigated the cytotoxicity of such polymersomes as well as the effects of nanosecond electric pulses with variable repetition rate on the shape and permeability of polymersomes in buffers with different conductivities...
July 22, 2017: Journal of Membrane Biology
https://www.readbyqxmd.com/read/28734932/a-pyridone-derivative-activates-serca2a-by-attenuating-the-inhibitory-effect-of-phospholamban
#16
Manami Kaneko, Hisato Yamamoto, Hiroki Sakai, Yusuke Kamada, Toshiki Tanaka, Shuji Fujiwara, Synsuke Yamamoto, Hiroki Takahagi, Hideyuki Igawa, Shizuo Kasai, Masakuni Noda, Makoto Inui, Tomoyuki Nishimoto
The cardiac sarco/endoplasmic reticulum Ca(2+)-dependent ATPase 2a (SERCA2a) plays a central role in Ca(2+) handling within cardiomyocytes and is negatively regulated by phospholamban (PLN), a sarcoplasmic reticulum (SR) membrane protein. The activation of SERCA2a, which has been reported to improve cardiac dysfunction in heart failure, is a potential therapeutic approach for heart failure. Therefore, we developed a novel small molecule, compound A and characterized it both in vitro and in vivo. Compound A activated the Ca(2+)-dependent ATPase activity of cardiac SR vesicles but not that of skeletal muscle SR vesicles that lack PLN...
July 19, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28734476/quantifying-the-interaction-between-egfr-dimers-and-grb2-in-live-cells
#17
Nuala Del Piccolo, Kalina Hristova
Adaptor proteins are a class of cytoplasmic proteins that bind to phosphorylated residues in receptor tyrosine kinases and trigger signaling cascades that control critically important cellular processes, such as cell survival, growth, differentiation, and motility. Here, we seek to characterize the interaction between epidermal growth factor receptor (EGFR) and the cytoplasmic adaptor protein growth factor receptor-bound protein 2 (Grb2) in a cellular context. To do so, we explore the utility of a highly biologically relevant model system, mammalian cells under reversible osmotic stress, and a recently introduced Förster resonance energy transfer microscopy method, fully quantified spectral imaging...
July 19, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28733628/solute-carriers-affect-anopheles-stephensi-survival-and-plasmodium-berghei-infection-in-the-salivary-glands
#18
J Couto, S Antunes, R Pinheiro-Silva, V do Rosário, J de la Fuente, A Domingos
Malaria is caused by mosquito-borne Plasmodium spp. parasites that must infect and survive within mosquito salivary glands (SGs) prior to host transmission. Recent advances in transcriptomics and the complete genome sequencing of mosquito vectors have increased our knowledge of the SG genes and proteins involved in pathogen infection and transmission. Membrane solute carriers are key proteins involved in drug transport and are useful in the development of new interventions for transmission blocking. Herein, we applied transcriptomics analysis to compare SGs mRNA levels in Anopheles stephensi fed on non-infected and P...
July 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28733553/exosomal-tetraspanins-mediate-cancer-metastasis-by-altering-host-microenvironment
#19
Jun Lu, Jun Li, Shuo Liu, Teng Wang, Alessandro Ianni, Eva Bober, Thomas Braun, Rong Xiang, Shijing Yue
The metastases of malignant tumors develop through a cascade of events. The establishment of a pre-metastatic micro-environment is initiated by communication between tumors and host. Exosomes come into focus as the most potent intercellular communicators playing a pivotal role in this process. Cancer cells release exosomes into the extracellular environment prior to metastasis. Tetraspanin is a type of 4 times transmembrane proteins. It may be involved in cell motility, adhesion, morphogenesis, as well as cell and vesicular membrane fusion...
July 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28733288/bacillomycin-d-produced-by-bacillus-amyloliquefaciens-is-involved-in-the-antagonistic-interaction-with-the-plant-pathogenic-fungus-fusarium-graminearum
#20
Qin Gu, Yang Yang, Qiming Yuan, Guangming Shi, Liming Wu, Zhiying Lou, Rong Huo, Huijun Wu, Rainer Borriss, Xuewen Gao
Fusarium graminearum (teleomorph: Ascomycota, Hypocreales, Gibberella, Gibberella zeae) is a destructive fungal pathogen that threatens the production and quality of wheat and barley worldwide. Controlling this toxin-producing pathogen is a significant challenge. In the present study, the commercially available strain Bacillus amyloliquefaciens (Bacteria, Firmicutes, Bacillales, Bacillus) FZB42 showed strong activity against F. graminearum The lipopeptide, bacillomycin D, produced by FZB42, was proven to contribute to the antifungal activity...
July 21, 2017: Applied and Environmental Microbiology
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