Read by QxMD icon Read


William A Harrell, Rebecca C Vieira, Susan M Ensel, Vicki Montgomery, Rebecca Guernieri, Vanessa S Eccard, Yvette Campbell, Virginia Roxas-Duncan, John H Cardellina, Robert P Webb, Leonard A Smith
Our initial discovery of 8-hydroxyquinoline inhibitors of BoNT/A and separation/testing of enantiomers of one of the more active leads indicated considerable flexibility in the binding site. We designed a limited study to investigate this flexibility and probe structure-activity relationships; utilizing the Betti reaction, a 36 compound matrix of quinolinol BoNT/A LC inhibitors was developed using three 8-hydroxyquinolines, three heteroaromatic amines, and four substituted benzaldehydes. This study has revealed some of the most effective quinolinol-based BoNT/A inhibitors to date, with 7 compounds displaying IC50 values ⩽1μM and 11 effective at ⩽2μM in an ex vivo assay...
November 9, 2016: Bioorganic & Medicinal Chemistry Letters
Alexa Childs, Clare Vesely, Leah Ensell, Helen Lowe, Tu Vinh Luong, Martyn E Caplin, Christos Toumpanakis, Christina Thirlwell, John A Hartley, Tim Meyer
BACKGROUND: Neuroendocrine tumours (NET) overexpress somatostatin receptors (SSTR) that can be targeted for therapy. Somatostatin receptor expression is routinely measured by molecular imaging but the resolution is insufficient to define heterogeneity. We hypothesised that SSTR expression could be measured on circulating tumour cells (CTCs) and used to investigate heterogeneity of expression and track changes during therapy. METHODS: MCF-7 cells were transfected with SSTR2 or 5 and spiked into donor blood for analysis by CellSearch...
December 6, 2016: British Journal of Cancer
Yu Jin Kim, Sungyoul Hong, Minjung Sung, Min Jeong Park, Kyungsoo Jung, Ka-Won Noh, Doo-Yi Oh, Mi-Sook Lee, Ensel Oh, Young Kee Shin, Yoon-La Choi
Therapies targeting SRC family kinases (SFKs) have shown efficacy in treating non-small cell lung cancer (NSCLC). However, recent clinical trials have found that the SFK inhibitor dasatinib is ineffective in some patient cohorts. Regardless, dasatinib treatment may benefit some NSCLC patient subgroups. Here, we investigated whether expression of LYN, a member of the SFK family, is associated with patient survival, the efficacy of dasatinib, and/or NSCLC cell viability. LYN expression was associated with poor overall survival in a multivariate analysis, and this association was strongest in non-smoker female patients with adenocarcinoma (ADC)...
October 14, 2016: Oncotarget
Lara Riehl, Alexander Seyboldt, Markus Ströbele, David Enseling, Thomas Jüstel, Michael Westberg, Peter R Ogilby, H-Jürgen Meyer
Octahedral tungsten iodide clusters equipped with apical ligands (L) are synthesized to implement substantial photophysical properties. The [W6I8(CF3COO)6](2-) cluster reported herein is the first example of a family of ligand substituted [W6I8L6](2-) clusters. Such compounds are expected to exhibit a rich photochemistry in which the apical ligands play a crucial role. The versatile solid state and solution phase photophysical properties of (TBA)2[W6I8(CF3COO)6] described herein parallel characteristics obtained in some photophysically active organic compounds, including a broad absorption in the UV/VIS region...
October 4, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
Shiyong Li, Yoon-La Choi, Zhuolin Gong, Xiao Liu, Maruja Lira, Zhengyan Kan, Ensel Oh, Jian Wang, Jason C Ting, Xiangsheng Ye, Christoph Reinhart, Xiaoqiao Liu, Yunfei Pei, Wei Zhou, Ronghua Chen, Shijun Fu, Gang Jin, Awei Jiang, Julio Fernandez, James Hardwick, Min Woong Kang, Hoseok I, Hancheng Zheng, Jhingook Kim, Mao Mao
INTRODUCTION: The incidence rate of lung adenocarcinoma (LUAD), the predominant histological subtype of lung cancer, is elevated in Asians, particularly in female nonsmokers. The mutation patterns in LUAD in Asians might be distinct from those in LUAD in whites. METHODS: We profiled 271 resected LUAD tumors (mainly stage I) to characterize the genomic landscape of LUAD in Asians with a focus on female nonsmokers. RESULTS: Mutations in EGFR, KRAS, erb-b2 receptor tyrosine kinase 2 gene (ERBB2), and BRAF; gene fusions involving anaplastic lymphoma receptor tyrosine kinase gene (ALK), ROS1, and ret proto-oncogene (RET); and Met Proto-Oncogene Tyrosine Kinase (MET) exon 14 skipping were the major drivers in LUAD in Asians, exhibiting mutually exclusive and differing prevalence from those reported in studies of LUAD in non-Asians...
December 2016: Journal of Thoracic Oncology
D Mandair, C Vesely, L Ensell, H Lowe, V Spanswick, J A Hartley, M E Caplin, T Meyer
No abstract text is available yet for this article.
October 2016: Endocrine-related Cancer
Hae Min Jeong, Sangseon Lee, Heejoon Chae, RyongNam Kim, Mi Jeong Kwon, Ensel Oh, Yoon-La Choi, Sun Kim, Young Kee Shin
AIMS: We compared four common methods for measuring DNA methylation levels and recommended the most efficient method in terms of cost and coverage. MATERIALS & METHODS: The DNA methylation status of liver and stomach tissues was profiled using four different methods, whole-genome bisulphite sequencing (WG-BS), targeted bisulphite sequencing (Targeted-BS), methylated DNA immunoprecipitation sequencing (MeDIP-seq) and methylated DNA immunoprecipitation bisulphite sequencing (MeDIP-BS)...
August 2016: Epigenomics
Carol Coohey, Scott D Easton
Depression is a common problem among homeless men that may interfere with functional tasks, such as securing stable housing, obtaining employment, and accessing health services. Previous research on depression among homeless men has largely focused on current psychosocial resources, substance abuse, and past victimization. Guided by Ensel and Lin's life course stress process model, the authors examined whether distal stressors, including victimization and exposure to parent problems in childhood, contributed to men's depression above and beyond current (or proximal) stressors, such as substance abuse and health problems, and social resources...
May 2016: Health & Social Work
Charlotte R Lemech, Leah Ensell, Jennifer C Paterson, Gemma Eminowicz, Helen Lowe, Rupali Arora, Hendrik-Tobias Arkenau, Martin Widschwendter, Nicola MacDonald, Adeola Olaitan, Tim Mould, Tim Meyer, John Hartley, Anita Mitra, Jonathan A Ledermann, Mary McCormack, Rebecca S Kristeleit
BACKGROUND: This is a feasibility study to determine whether circulating tumour cells (CTCs) are detectable and suitable for molecular profiling in advanced endometrial cancer (aEC). METHOD: Between October 2012 and February 2014, 30 patients with aEC had baseline and up to 3 follow-up samples. CTCs and stathmin expression were evaluated using the CellSearch platform. Epithelial cell adhesion molecule (EpCAM) and stathmin immunohistochemistry were performed on FFPE tumour tissue...
2016: Oncology
Gab-Yong Bae, Soon-Ki Hong, Jeong-Rak Park, Ok-Seon Kwon, Keun-Tae Kim, JaeHyung Koo, Ensel Oh, Hyuk-Jin Cha
Chronic exposure to TGFβ, a frequent occurrence for tumor cells in the tumor microenvironment, confers more aggressive phenotypes on cancer cells by promoting their invasion and migration while at the same time increasing their resistance to the growth-inhibitory effect of TGFβ. In this study, a transdifferentiated (TD) A549 cell model, established by chronically exposing A549 cells to TGFβ, showed highly invasive phenotypes in conjunction with attenuation of Smad-dependent signaling. We show that Snail protein, the mRNA expression of which strongly correlates with a poor prognosis in lung cancer patients, was highly stable in TD cells after TGFβ stimulation...
May 3, 2016: Oncotarget
Seung Eun Lee, Ensel Oh, Boram Lee, Yu Jin Kim, Doo-Yi Oh, Kyungsoo Jung, Jong-Sun Choi, Junghan Kim, Sung Joo Kim, Jung Wook Yang, Jungsuk An, Young Lyun Oh, Yoon La Choi
Malignant pheochromocytoma/paraganglioma (PCC/PGL) is defined by the presence of metastases at non-chromaffin sites, which makes it difficult to prospectively diagnose malignancy. Here, we performed array CGH (aCGH) and paired gene expression profiling of fresh, frozen PCC/PGL samples (n = 12), including three malignant tumors, to identify genes that distinguish benign from malignant tumors. Most PCC/PGL cases showed few copy number aberrations, regardless of malignancy status, but mRNA analysis revealed that 390 genes were differentially expressed in benign and malignant tumors...
April 26, 2016: Oncotarget
Andrew R McNamara, Scott Ensell, Timothy D Farley
Friction blisters are a common sequela of many athletic activities. Their significance can range from minor annoyance to major performance disruptions. The latter is particularly true in baseball pitchers, who sustain repeated trauma between the baseball seams and the fingers of the pitching hand, predominately at the tips of the index and long fingers. Since 2010, 6 Major League Baseball (MLB) players accounted for 7 stints on the disabled list (DL) due to blisters. These injuries resulted in a total of 151 days spent on the DL...
March 2016: American Journal of Orthopedics
Ensel Oh, Yoon-La Choi, Mi Jeong Kwon, Ryong Nam Kim, Yu Jin Kim, Ji-Young Song, Kyung Soo Jung, Young Kee Shin
Formalin fixing with paraffin embedding (FFPE) has been a standard sample preparation method for decades, and archival FFPE samples are still very useful resources. Nonetheless, the use of FFPE samples in cancer genome analysis using next-generation sequencing, which is a powerful technique for the identification of genomic alterations at the nucleotide level, has been challenging due to poor DNA quality and artificial sequence alterations. In this study, we performed whole-exome sequencing of matched frozen samples and FFPE samples of tissues from 4 cancer patients and compared the next-generation sequencing data obtained from these samples...
2015: PloS One
Ok-Seon Kwon, Ensel Oh, Jeong-Rak Park, Ji-Seon Lee, Gab-Yong Bae, Jae-Hyung Koo, Hyongbum Kim, Yoon L Choi, Young Soo Choi, Jhingook Kim, Hyuk-Jin Cha
While metastasis, the main cause of lung cancer-related death, has been extensively studied, the underlying molecular mechanism remains unclear. A previous clinicogenomic study revealed that expression of N-acetylgalactosaminyltransferase (GalNAc-T14), is highly inversely correlated with recurrence-free survival in those with non-small cell lung cancer (NSCLC). However, the underlying molecular mechanism(s) has not been determined. Here, we showed that GalNAc-T14 expression was positively associated with the invasive phenotype...
December 8, 2015: Oncotarget
Doo-Yi Oh, Seokhwi Kim, Yoon-La Choi, Young Jae Cho, Ensel Oh, Jung-Joo Choi, Kyungsoo Jung, Ji-Young Song, Suzie E Ahn, Byoung-Gie Kim, Duk-Soo Bae, Woong-Yang Park, Jeong-Won Lee, Sangyong Song
Surgery and radiation are the current standard treatments for cervical cancer. However, there is no effective therapy for metastatic or recurrent cases, necessitating the identification of therapeutic targets. In order to create preclinical models for screening potential therapeutic targets, we established 14 patient-derived xenograft (PDX) models of cervical cancers using subrenal implantation methods. Serially passaged PDX tumors retained the histopathologic and genomic features of the original tumors. Among the 9 molecularly profiled cervical cancer patient samples, a HER2-amplified tumor was detected by array comparative genomic hybridization and targeted next-generation sequencing...
November 3, 2015: Oncotarget
M Kubus, K Levin, S Kroeker, D Enseling, T Jüstel, H-J Meyer
CaMg(2)AlN(3) was synthesized in a closed system by solid state reaction from binary nitrides. Structure refinements based on powder X-ray diffraction data suggested ambiguity about the occupancy of magnesium and aluminum tetrahedral sites. Solid-state (27)Al and (25)Mg NMR studies were used to adjudicate amongst possible space groups. With reference to projector augmented wave calculations of the quadrupolar coupling constants, the measured values of CQ and the numbers of crystallographically inequivalent Al and Mg sites indicate that CaMg(2)AlN(3) crystallizes in the space group P6(3)/mmc with partial occupancy of the distorted tetrahedral Al site and possibly also mixing of Mg(2+) and Al(3+) ions on opposite sites...
February 14, 2015: Dalton Transactions: An International Journal of Inorganic Chemistry
Yu Jin Kim, Jong-Sun Choi, Jinwon Seo, Ji-Young Song, Seung Eun Lee, Mi Jung Kwon, Mi Jeong Kwon, Juthika Kundu, Kyungsoo Jung, Ensel Oh, Young Kee Shin, Yoon-La Choi
MET, a cell surface receptor for hepatocyte growth factor, is involved in the development of triple-negative/basal-like breast cancer (TNBC/BLBC). However, its utility as a therapeutic target in this subtype of breast cancer is poorly understood. To evaluate MET fully as a potential therapeutic target for TNBC/BLBC, we investigated the relationship between MET expression and clinical outcomes of patients with breast cancer and the functional effect of MET inhibition. Using automated immunohistochemistry (Ventana), we analyzed MET expression in 924 breast cancer patients with relevant clinicopathologic parameters...
May 15, 2014: International Journal of Cancer. Journal International du Cancer
Mineui Hong, Ryong Nam Kim, Ji-Young Song, So-Jung Choi, Ensel Oh, Maruja E Lira, Mao Mao, Kengo Takeuchi, Joungho Han, Jhingook Kim, Yoon-La Choi
INTRODUCTION: The most common mechanism underlying overexpression and activation of anaplastic lymphoma kinase (ALK) in non-small-cell lung carcinoma could be attributed to the formation of a fusion protein. To date, five fusion partners of ALK have been reported, namely, echinoderm microtubule associated protein like 4, tropomyosin-related kinase-fused gene, kinesin family member 5B, kinesin light chain 1, and protein tyrosine phosphatase, nonreceptor type 3. METHODS: In this article, we report a novel fusion gene huntingtin interacting protein 1 (HIP1)-ALK, which is conjoined between the huntingtin-interacting protein 1 gene HIP1 and ALK...
March 2014: Journal of Thoracic Oncology
Silvia Park, Jeeyun Lee, In-Gu Do, Jiryeon Jang, Kyoohyoung Rho, Seonjoo Ahn, Lira Maruja, Sung Joo Kim, Kyoung-Mee Kim, Mao Mao, Ensel Oh, Yu Jin Kim, Jhingook Kim, Yoon-La Choi
Rhabdomyosarcoma (RMS) is the most commonly occurring type of soft tissue tumor in children. However, it is rare in adults, and therefore, very little is known about the most appropriate treatment strategy for adult RMS patients. We performed genomic analysis of RMS cells derived from a 27-year-old male patient whose disease was refractory to treatment. A peritoneal seeding nodule from the primary tumor, pleural metastases, malignant pleural effusion, and ascites obtained during disease progression, were analyzed...
January 10, 2014: Scientific Reports
Yong Beom Cho, Hye Kyung Hong, Yoon-La Choi, Ensel Oh, Kyeung Min Joo, Juyoun Jin, Do-Hyun Nam, Young-Hyeh Ko, Woo Yong Lee
BACKGROUND: Despite significant improvements in colon cancer outcomes over the past few decades, preclinical development of more effective therapeutic strategies is still limited by the availability of clinically relevant animal models. To meet those clinical unmet needs, we generated a well-characterized in vivo preclinical platform for colorectal cancer using fresh surgical samples. METHODS: Primary and metastatic colorectal tumor tissues (1-2 mm(3)) that originate from surgery were implanted into the subcutaneous space of nude mice and serially passaged in vivo...
April 2014: Journal of Surgical Research
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"