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https://www.readbyqxmd.com/read/27911481/photoluminescence-and-thermoluminescence-of-k2-mg-so4-2-eu-and-evaluation-of-its-kinetic-parameters
#1
Archana Deshpande, N S Dhoble, S C Gedam, S J Dhoble
The K2 Mg(SO4 )2 :Eu phosphor, synthesized by a solid-state diffusion method, was studied for its photoluminescence (PL) and thermoluminescence (TL) characteristics. The X-ray diffraction (XRD) pattern of the material was matched with the standard JCPDF No. 36-1499. For PL characteristics, K2 Mg(SO4 )2 :Eu(2)(+) showed an emission peak at 474 nm when excited at 340 nm, while it showed Eu(3)(+) emission at 580 nm, and 594 nm splitting at 613 nm and 618 nm for an excitation of 396 nm wavelength due to radiative transitions from (5) D0 to (7) Fj (j = 0, 1, 2, 3)...
December 2, 2016: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/27909721/protective-effect-of-vk2-on-glucocorticoid-treated-mc3t3-e1-cells
#2
Yue-Lei Zhang, Jun-Hui Yin, Hao Ding, Wei Zhang, Chang-Qing Zhang, You-Shui Gao
Glucocorticoids (GCs) contribute to the increased incidence of secondary osteoporosis and osteonecrosis, and medications for the prevention and treatment of these complications have been investigated for many years. Vitamin K2 (VK2) has been proven to promote bone formation both in vitro and in vivo. In this study, we examined the effects of VK2 on dexamethasone (DEX)-treated MC3T3-E1 osteoblastic cells. We observed that VK2 promoted the proliferation and enhanced the survival of dexamethasone-treated MC3T3-E1 cells...
December 1, 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27902346/hymenobacter-rutilus-sp-nov-isolated-from-marine-sediment-in-arctic
#3
Myong Chol Kim, Chol Myong Kim, Ok Chol Kang, Yumin Zhang, Zuobing Liu, Danzeng Wangmu, Ziyan Wei, Yao Huang, Fang Peng
Strain K2-33028T, appeared brick-red colony on R2A plate, was isolated from marine sediment sample in Kings Bay, Svalbard Archipelago, Norway. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K2-33028T belonged to the genus Hymenobacter. Cells were Gram-reaction-negative, non-spore-forming, aerobic, rod-shaped bacterium without motility. Growth occurred at 4-37℃ (optimum 28℃) and at pH 6.0-8.0 (optimum pH 7.0). Cells contained menaquinone-7, as the main respiratory quinone, and iso-C15:0, summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), C16:1 ω5c, summed feature 4 (comprising anteiso-C17:1 B and/or iso-C17:1 I) and anteiso-C15:0 as the major cellular fatty acids...
November 21, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27900479/evaluation-of-the-repeatability-of-a-swept-source-ocular-biometer-for-measuring-ocular-biometric-parameters
#4
Teresa Ferrer-Blasco, Alberto Domínguez-Vicent, José J Esteve-Taboada, Miguel A Aloy, Jose E Adsuara, Robert Montés-Micó
PURPOSE: The purpose was to evaluate the repeatability of a new swept-source optical biometer for measuring ocular biometric parameters. METHODS: Thirty subjects with healthy and phakic eyes were included in this study, and only one eye per participant was analysed. Each eye was measured five times with the IOLMaster 700 swept-source optical biometer (Carl Zeiss Meditec, Jena, Germany). Axial length (AL), anterior chamber depth (ACD), central corneal thickness (CCT), lens thickness (LT), white-to-white (WTW), and K1 and K2 keratometric readings were evaluated...
November 30, 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/27899371/refractive-improvements-and-safety-with-topography-guided-corneal-crosslinking-for-keratoconus-1-year-results
#5
Maria Nordström, Maria Schiller, Anneli Fredriksson, Anders Behndig
PURPOSE: To assess the refractive improvements and the corneal endothelial safety of an individualised topography-guided regimen for corneal crosslinking in progressive keratoconus. METHODS: An open-label prospective randomised clinical trial was performed at the Department of Clinical Sciences, Ophthalmology, Umeå University Hospital, Umeå, Sweden. Thirty-seven patients (50 eyes) with progressive keratoconus planned for corneal crosslinking were included. The patients were randomised to topography-guided crosslinking (photorefractive intrastromal crosslinking (PiXL); n=25) or uniform 9 mm crosslinking (corneal collagen crosslinking (CXL); n=25)...
November 29, 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/27896836/radiosynthesis-and-biological-evaluation-of-the-new-pde10a-radioligand-18-f-aq28a
#6
Sally Wagner, Rodrigo Teodoro, Winnie Deuther-Conrad, Mathias Kranz, Matthias Scheunemann, Steffen Fischer, Barbara Wenzel, Ute Egerland, Norbert Hoefgen, Jörg Steinbach, Peter Brust
Cyclic nucleotide phosphodiesterase 10A (PDE10A) regulates the level of the second messengers cAMP and cGMP in particular in brain regions assumed to be associated with neurodegenerative and psychiatric diseases. A better understanding of the pathophysiological role of the expression of PDE10A could be obtained by quantitative imaging of the enzyme by positron emission tomography (PET). Thus, in this study we developed, radiolabeled, and evaluated a new PDE10A radioligand, 8-bromo-1-(6-[(18) F]fluoropyridin-3-yl)-3,4-dimethylimidazo[1,5-a]quinoxaline ([(18) F]AQ28A)...
November 29, 2016: Journal of Labelled Compounds & Radiopharmaceuticals
https://www.readbyqxmd.com/read/27875886/an-accurate-potential-model-for-the-a-3-%C3%AE-u-state-of-the-alkali-dimers-na2-k2-rb2-and-cs2
#7
Jascha A Lau, J Peter Toennies, K T Tang
A modified semi-empirical Tang-Toennies potential model is used to describe the a(3)Σu(+) potentials of the alkali dimers. These potentials are currently of interest in connection with the laser manipulation of the ultracold alkali gases. The fully analytical model is based on three experimental parameters, the well depth De, well location Re, and the harmonic vibrational frequency ωe of which the latter is only slightly optimized within the range of the literature values. Comparison with the latest spectroscopic data shows good agreement for Na2, K2, Rb2, and Cs2, comparable to that found with published potential models with up to 55 parameters...
November 21, 2016: Journal of Chemical Physics
https://www.readbyqxmd.com/read/27874032/connexin-32-mediated-cell-cell-communication-is-essential-for-hepatic-differentiation-from-human-embryonic-stem-cells
#8
Jinhua Qin, Mingyang Chang, Shuyong Wang, Zhenbo Liu, Wei Zhu, Yi Wang, Fang Yan, Jian Li, Bowen Zhang, Guifang Dou, Jiang Liu, Xuetao Pei, Yunfang Wang
Gap junction-mediated cell-cell interactions are highly conserved and play essential roles in cell survival, proliferation, differentiation and patterning. We report that Connexin 32 (Cx32)-mediated gap junctional intercellular communication (GJIC) is necessary for human embryonic stem cell-derived hepatocytes (hESC-Heps) during step-wise hepatic lineage restriction and maturation. Vitamin K2, previously shown to promote Cx32 expression in mature hepatocytes, up-regulated Cx32 expression and GJIC activation during hepatic differentiation and maturation, resulting in significant increases of hepatic markers expression and hepatocyte functions...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27870069/suspected-myelodysplastic-myeloproliferative-neoplasm-in-a-feline-leukemia-virus-negative-cat
#9
Amy L Weeden, Kyle R Taylor, Scott P Terrell, Alexander E Gallagher, Heather L Wamsley
A 10-year-old castrated Domestic Short-Haired cat was presented to a primary care veterinarian for a wellness examination and laboratory examination for monitoring of diabetes mellitus. The CBC revealed marked thrombocytosis, leukopenia and macrocytic, normochromic anemia. The cat tested negative for FeLV and feline immunodeficiency virus, but was positive for Mycoplasma haemominutum by PCR. Hematologic abnormalities were not responsive to therapy, so a repeat CBC and a bone marrow aspiration for cytology were performed...
November 21, 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27865621/menaquinone-4-vitamin-k2-up-regulates-expression-of-human-intestinal-alkaline-phosphatase-in-caco-2-cells
#10
Seiko Noda, Asako Yamada, Rieko Tanabe, Kanae Nakaoka, Takayuki Hosoi, Masae Goseki-Sone
Alkaline phosphatase (ALP) hydrolyzes several monophosphate esters into inorganic acid and alcohol. In humans, 4 kinds of ALP isozymes have been identified: tissue-nonspecific ALP, intestinal ALP, placental ALP, and germ cell ALP. Intestinal ALP is expressed at a high concentration in the brush border membrane of intestinal epithelial cells and is known to be affected by several kinds of nutrients, such as lipids, but the physiological function of intestinal ALP has remained elusive. Vitamin K is an essential cofactor for the posttranslational carboxylation of glutamate residues into γ-carboxy glutamate...
October 14, 2016: Nutrition Research
https://www.readbyqxmd.com/read/27862738/catalytic-asymmetric-synthesis-of-3-indolyl-methanamines-using-unprotected-indoles-and-n-boc-imines-under-basic-conditions
#11
Takayoshi Arai, Junki Kakino
A chiral imidazolidine-containing NCN/Pd-OTf catalyst (C4) promoted the nucleophilic addition of unprotected indoles to N-Boc imines. Using sulfinyl amines as the N-Boc imine precursors, the combined use of C4 with K2 CO3 activated the NH indoles to give chiral 3-indolyl methanamines with up to 98 % ee. Compared with conventional acid-catalyzed Friedel-Crafts reactions, this reaction proceeds under mildly basic conditions and is advantageous for the use of acid-sensitive substrates.
November 10, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27862288/commercially-available-enzyme-linked-immunosorbent-assay-and-polymerase-chain-reaction-tests-for-detection-of-feline-immunodeficiency-virus-infection
#12
J Nichols, H Y Weng, A Litster, C Leutenegger, L Guptill
BACKGROUND: Feline immunodeficiency virus (FIV) infection is an important cause of disease of cats worldwide. Initial screening is commonly performed by commercially available point-of-care (POC) ELISA tests. Confirmatory testing for positive POC test results is recommended. Polymerase chain reaction (PCR) tests for FIV are commonly used additional testing methods; however, reported measures of diagnostic accuracy vary widely between PCR tests, making interpretation of results difficult...
November 15, 2016: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/27856233/liars-reporter-assay-a-simple-tool-to-identify-lipid-ii-binding-moieties-in-lantibiotic-nukacin-isk-1
#13
Khaled M Elsayed, Mohammad R Islam, Abdullah-Al-Mahin, Jun-Ichi Nagao, Takeshi Zendo, Kenji Sonomoto
Binding to lipid II is an important step in the mode of action of most lantibiotics targeting the bacterial cell wall. We applied the Bacillus subtilis two-component system, LiaRS, that is known to respond to antibiotics interfering with lipid II cycle, in order to evaluate lipid II binding activity of known bacteriocins and also to identify lipid II binding moieties in lantibiotic nukacin ISK-1. Using this method, we confirmed that the methyllanthionine ring in nukacin ISK-1 is crucial for lipid II binding as previously indicated...
November 14, 2016: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/27855333/transport-and-aggregation-of-rutile-titanium-dioxide-nanoparticles-in-saturated-porous-media-in-the-presence-of-ammonium
#14
Xiaoting Xu, Nan Xu, Xueying Cheng, Peng Guo, Zhigang Chen, Dongtian Wang
The widely used artificial nanoparticles (NPs) and the excess of ammonium (NH4(+)) fertilizers are easily released into the natural environment. So, clarifying the mobility of NPs in the presence of NH4(+) is therefore of great urgency and high priority. Currently, few studies focus on the transport and deposition of nanoparticle titanium dioxide (nTiO2) in single and binary systems containing NH4(+), especially describing this process by a mathematical model. In this work, the comparison between the transport and retention of rutile nTiO2 in single and binary electrolyte solutions of NH4Cl and/or NaCl (0...
November 14, 2016: Chemosphere
https://www.readbyqxmd.com/read/27851930/biophysical-characterization-of-the-interaction-between-m2-1-protein-of-hrsv-and-quercetin
#15
Thiago Salem Pançonato Teixeira, Ícaro Putinhon Caruso, Bruno Rafael Pereira Lopes, Luis Octávio Regasini, Karina Alves de Toledo, Marcelo Andrés Fossey, Fátima Pereira de Souza
hRSV is the major causative agent of acute respiratory infections. Among its eleven proteins, M2-1 is a transcription antiterminator, making it an interesting target for antivirals. Quercetin is a flavonol which inhibits some virus infectivity and replication. In the present work, the M2-1 gene was cloned, expressed and the protein was purified. Thermal stability and secondary structure were analyzed by circular dichroism and the interaction with Quercetin was evaluated by fluorescence spectroscopy. Molecular docking experiments were performed to understand this mechanism of interaction...
November 13, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27850472/834-k2-the-deadly-designer-drug-a-multicenter-case-series-on-synthetic-cannabinoid-complications
#16
Faith Armstrong, Michael McCurdy, Elizabeth Otto
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27847219/biomonitoring-of-29-trace-elements-in-whole-blood-from-inhabitants-of-cotonou-benin-by-icp-ms
#17
Brice Yedomon, Alain Menudier, Florence Lecavelier Des Etangs, Ludovic Anani, Benjamin Fayomi, Michel Druet-Cabanac, Christian Moesch
This study aimed to investigate the blood concentration of 29 trace elements, metals or metalloids, in a healthy population of Cotonou not directly exposed to metals in order to propose reference values. Blood samples from 70 blood donors were collected in K2 EDTA tubes for trace elements during September 2015 and a questionnaire was used to assess lifestyle exposure. Blood metal concentrations were determined by inductively coupled plasma mass spectrometry (ICP-MS) equipped with a quadrupole-based reaction cell...
November 9, 2016: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/27833747/microbiota-host-interplay-at-the-gut-epithelial-level-health-and-nutrition
#18
REVIEW
Jean-Paul Lallès
Growing evidence suggests the implication of the gut microbiota in various facets of health and disease. In this review, the focus is put on microbiota-host molecular cross-talk at the gut epithelial level with special emphasis on two defense systems: intestinal alkaline phosphatase (IAP) and inducible heat shock proteins (iHSPs). Both IAP and iHSPs are induced by various microbial structural components (e.g. lipopolysaccharide, flagellin, CpG DNA motifs), metabolites (e.g. n-butyrate) or secreted signal molecules (e...
2016: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/27832506/production-and-application-of-menaquinone-7-vitamin-k2-a-new-perspective
#19
REVIEW
Ehsan Mahdinia, Ali Demirci, Aydin Berenjian
Menaquinone-7, a highly valuable member of the vitamin K series, has significant effects on preventing osteoporosis and cardiovascular disease besides its positive effects on blood coagulation. In this review, chemical and biological aspects of menaquinone-7 are briefly summarized followed by a critical review on upstream and downstream processing developments for its production and recovery, including solid versus liquid fermentations, static versus agitated fermentations and online versus post-production recovery...
January 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27831606/-determination-of-the-healing-effect-of-piper-aduncum-spiked-pepper-or-matico-on-human-fibroblasts
#20
Karen Paco, Luis Alberto Ponce-Soto, Marco Lopez-Ilasaca, José L Aguilar
Objectives.: To evaluate the healing effect of a Piper aduncum ethanol-water extract on an adult human dermal fibroblast cell line (hDFa). Materials and Methods.: After obtaining the extract via solid-liquid extraction, concentration, and lyophilization, extract proteins were purified using reverse phase high-performance liquid chromatography, identified using tandem mass spectrometry of tryptic peptides, and analyzed using MALDI-TOF-TOF on an ABSciex4800 mass spectrometer...
July 2016: Revista Peruana de Medicina Experimental y Salud Pública
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