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https://www.readbyqxmd.com/read/29230357/comparative-analyses-of-putative-toxin-gene-homologs-from-an-old-world-viper-daboia-russelii
#1
Neeraja M Krishnan, Binay Panda
Availability of snake genome sequences has opened up exciting areas of research on comparative genomics and gene diversity. One of the challenges in studying snake genomes is the acquisition of biological material from live animals, especially from the venomous ones, making the process cumbersome and time-consuming. Here, we report comparative sequence analyses of putative toxin gene homologs from Russell's viper (Daboia russelii) using whole-genome sequencing data obtained from shed skin. When compared with the major venom proteins in Russell's viper studied previously, we found 45-100% sequence similarity between the venom proteins and their putative homologs in the skin...
2017: PeerJ
https://www.readbyqxmd.com/read/29230010/serotype-and-virulence-genes-of-klebsiella-pneumoniae-isolated-from-mink-and-its-pathogenesis-in-mice-and-mink
#2
Wang Jian-Li, Shang Yuan-Yuan, Guo Shou-Yu, Diao Fei-Fei, Yu Jia-Yu, Wei Xue-Hua, Zhao Yong-Feng, Jiang Shi-Jin, Xie Zhi-Jing
In the study, 15 K. pneumoniae strains were isolated from the mink experiencing respiratory distress in mideastern Shandong province, China, and the prevalence of K. pneumoniae in the sampled mink was 11.9% (15/126). Fourteen (93.33%) of the 15 K. pneumoniae isolates were identified as serotype K2 and hypermucoviscosity phenotype. The 12 virulence-associated genes of the K. pneumoniae isolates were tested. The prevalence of the wabG gene for the isolates were 100% (15/15), the ureA gene 100% (15/15), the rmpA gene 93...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29226932/ligand-induced-decarbonylation-in-diphosphine-ligated-palladium-acetates-ch3co2pd-pr2-2ch2-r-me-and-ph
#3
Michael Lesslie, Yang Yang, Allan J Canty, Elettra Piacentino, Francis Berthias, Philippe Maitre, Victor Ryzhov, Richard A J O'Hair
A new decarbonylation reaction is observed for [(K2-acetate)Pd(K2-diphosphine)]+ complexes. Gas-phase IR experiments identify the product as [CH3Pd(OP(Ph2)CH2PPh2)]+. DFT calculations uncovered a plausible mechanism involving O atom abstraction by the diphosphine ligand within the coordination sphere to yield the acetyl complex, [CH3COPd(OP(Ph2)CH2PPh2)]+, which then undergoes decarbonylation.
December 11, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29220089/potato-plants-solanum-tuberosum-l-are-chloride-sensitive-is-this-dogma-valid
#4
Birgit W Hütsch, Katrin Keipp, Ann-Kathrin Glaser, Sven Schubert
BACKGROUND: Chloride sensitivity of the potato (Solanum tuberosum L.) cultivars Marabel and Désirée was investigated in two pot experiments (soil/sand mixture and hydroponics). It was tested whether there are differential effects of KCl and K2 SO4 application on tuber yield and tuber quality, and whether both potato cultivars differ in their chloride sensitivity. RESULTS: Tuber yield, dry matter percentage of the tubers, starch concentration and starch yield were not significantly affected by potassium source (K2 SO4 or KCl)...
December 8, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29219951/comparative-study-of-long-term-outcomes-of-accelerated-and-conventional-collagen-crosslinking-for-progressive-keratoconus
#5
J J Males, D Viswanathan
PurposeTo compare the long-term outcomes of accelerated corneal collagen crosslinking (CXL) to conventional CXL for progressive keratoconus.Patients and methodsComparative clinical study of consecutive progressive keratoconic eyes that underwent either accelerated CXL (9 mW/cm2 ultraviolet A (UVA) light irradiance for 10 min) or conventional CXL (3 mW/cm2 UVA light irradiance for 30 min). Eyes with minimum 12 months' follow-up were included. Post-procedure changes in keratometry readings (Flat meridian: K1; steep meridian: K2), central corneal thickness (CCT), best spectacle-corrected visual acuity (BSCVA), and manifest refraction spherical equivalent (MRSE) were analysed...
December 8, 2017: Eye
https://www.readbyqxmd.com/read/29214795/retraction-paper-comparison-of-the-effect-of-vitamin-k2-and-risedronate-on-trabecular-bone-in-glucocorticoid-treated-rats-a-bone-histomorphometry-study-by-iwamoto-j-et-al-yonsei-med-j-2009-50-2-189-194
#6
https://www.readbyqxmd.com/read/29213118/vitamin-k2-prevents-lymphoma-in-drosophila
#7
Maytham A Dragh, Zhiliang Xu, Zainab S Al-Allak, Ling Hong
Previous studies have established the anticancer effect of vitamin K2 (VK2). However, its effect on lymphoma induced by UBIAD1/heix mutation in Drosophila remains unknown. Therefore, we aimed to develop an in vivo model of lymphoma for the precise characterization of lymphoma phenotypes. We also aimed to improve the understanding of the mechanisms that underlie the preventative effects of VK2 on lymphoma. Our results demonstrated that VK2 prevents lymphoma by acting as an electron carrier and by correcting the function and structure of mitochondria by inhibiting mitochondrial reactive oxygen species production mtROS...
December 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29211812/a-simulation-study-on-the-impact-of-the-blood-flow-dependent-component-in-18f-av45-suvr-in-alzheimer-s-disease
#8
Julie Ottoy, Jeroen Verhaeghe, Ellis Niemantsverdriet, Sebastiaan Engelborghs, Sigrid Stroobants, Steven Staelens
BACKGROUND: Increased brain uptake on [18F]AV45 PET is a biomarker for Alzheimer's disease (AD). The standardised uptake value ratio (SUVR) is widely used for quantification but is subject to variability. Here we evaluate how SUVR of a cortical target region is affected by blood flow changes in the target and two frequently used reference regions. METHODS: Regional baseline time-activity curves (TACs) were simulated based on metabolite-corrected plasma input functions and pharmacokinetic parameters obtained from our previously acquired data in healthy control (HC; n = 10), amnestic mild cognitive impairment (aMCI; n = 15) and AD cohorts (n = 9)...
2017: PloS One
https://www.readbyqxmd.com/read/29206117/synthesis-crystal-structures-and-properties-of-the-new-compounds-k7-xag1-x-xo4-4-x-mo-w
#9
Tatyana S Spiridonova, Sergey F Solodovnikov, Aleksandra A Savina, Zoya A Solodovnikova, Sergey Yu Stefanovich, Bogdan I Lazoryak, Iliya V Korolkov, Elena G Khaikina
Two new isostructural compounds, namely heptapotassium silver tetrakis(tetraoxomolybdate), K7-xAg1+x(MoO4)4 (0 ≤ x ≤ 0.4), and heptapotassium silver tetrakis(tetraoxotungstate), K7-xAg1+x(WO4)4 (0 ≤ x ≤ 0.4), have been synthesized and found to crystallize in the polar space group P63mc (Z = 2) with the unit-cell dimensions a = 12.4188 (2) and c = 7.4338 (2) Å for K6.68Ag1.32(MoO4)4 (single-crystal data), and a = 12.4912 (5) and c = 7.4526 (3) Å for K7Ag(WO4)4 (Rietveld analysis data). Both structures represent a new structure type, with characteristic [K1(XO4)6] `pinwheels' of K1O6 octahedra and six XO4 tetrahedra (X = Mo, W) connected by common opposite faces into columns along the c axes...
December 1, 2017: Acta Crystallographica. Section C, Structural Chemistry
https://www.readbyqxmd.com/read/29205407/effects-of-the-acute-and-chronic-ethanol-intoxication-on-acetate-metabolism-and-kinetics-in-the-rat-brain
#10
Ya-Ju Hsieh, Liang-Chih Wu, Chien-Chih Ke, Chi-Wei Chang, Jung-Wen Kuo, Wen-Sheng Huang, Fu-Du Chen, Bang-Hung Yang, Hsiao-Ting Tai, Sharon Chia-Ju Chen, Ren-Shyan Liu
BACKGROUND: Ethanol intoxication inhibits glucose transport and decreases overall brain glucose metabolism; however, humans with long-term ethanol consumption were found to have a significant increase in [1-11 C]-acetate uptake in the brain. The relationship between the cause and effect of [1-11 C]-acetate kinetics and acute/chronic ethanol intoxication, however, are still unclear. METHODS: [1-11 C]-acetate positron emission tomography (PET) with dynamic measurement of K1 and k2 rate constants was used to investigate the changes in acetate metabolism in different brain regions of rats with acute or chronic ethanol intoxication...
December 5, 2017: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/29205138/virulence-genes-in-isolates-of-klebsiella-pneumoniae-from-the-uk-during-2016-including-among-carbapenemase-gene-positive-hypervirulent-k1-st23-and-non-hypervirulent-types-st147-st15-and-st383
#11
Jane F Turton, Zoë Payne, Amy Coward, Katie L Hopkins, Jack A Turton, Michel Doumith, Neil Woodford
PURPOSE: Klebsiella pneumoniae is a concern because of its multidrug resistance and the ability of hypervirulent types, especially capsular type K1-clonal complex 23 (K1-CC23), to cause community-acquired, life-threatening infections. Hypervirulent types carry an array of virulence genes including rmpA/rmpA2, coding for capsule up-regulation. We sought to identify isolates carrying these elements among submissions to the UK national reference laboratory during 2016. METHODOLOGY: Virulence elements and carbapenemase genes were sought by PCR or from whole genome sequences...
December 5, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29202321/quantitative-analysis-of-breast-cancer-diagnosis-using-a-probabilistic-modelling-approach
#12
Shuo Liu, Jinshu Zeng, Huizhou Gong, Hongqin Yang, Jia Zhai, Yi Cao, Junxiu Liu, Yuling Luo, Yuhua Li, Liam Maguire, Xuemei Ding
BACKGROUND: Breast cancer is the most prevalent cancer in women in most countries of the world. Many computer-aided diagnostic methods have been proposed, but there are few studies on quantitative discovery of probabilistic dependencies among breast cancer data features and identification of the contribution of each feature to breast cancer diagnosis. METHODS: This study aims to fill this void by utilizing a Bayesian network (BN) modelling approach. A K2 learning algorithm and statistical computation methods are used to construct BN structure and assess the obtained BN model...
November 21, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/29196151/effect-of-vitamin-k2-on-type-2-diabetes-mellitus-a-review
#13
REVIEW
Yan Li, Jie Peng Chen, Lili Duan, Shuzhuang Li
Type 2 diabetes mellitus (T2DM) continue to be a major public health problem around the world that frequently presents with microvascular and macrovascular complications. Individuals with T2DM are not only suffering from significant emotional and physical misery, but also at increased risk of dying from severe complications. In recent years, evidence from prospective observational studies and clinical trials has shown T2DM risk reduction with vitamin K2 supplementation. We thus did an overview of currently available studies to assess the effect of vitamin K2 supplementation on insulin sensitivity, glycaemic control and reviewed the underlying mechanisms...
November 28, 2017: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/29194040/effect-of-preanalytical-and-analytical-variables-on-the-clinical-utility-of-mean-platelet-volume
#14
Mauro Buttarello, Giacomo Mezzapelle, Mario Plebani
BACKGROUND: The study endpoint was to analyze the effect of preanalytical (time, temperature, anticoagulant) and analytical (imprecision, correlation between volume and platelet concentration) variables on mean platelet volume (MPV). A further aim was to calculate in an adult population the reference intervals using the Sysmex XE-5000 analyzer. A critical evaluation was also made of the clinical utility of these parameters. METHODS: Analyses of the above values were performed in duplicate in 170 healthy adults of both sexes: (1) within 30 min from collection, and (2) after 4 h...
December 1, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29190485/removing-the-residual-cellulase-by-graphene-oxide-to-recycle-the-bio-polishing-effluent-for-dyeing-cotton-fabrics
#15
Rui Wang, Chao Yang, Kuanjun Fang, Yuqing Cai, Longyun Hao
In this research, a stable graphene oxide (GO) suspension was prepared by chemical reduction method from graphite powder. By TEM, the irregular GO sheets with single-atom-layered structure could be observed. The zeta potentials measurement indicated the surface charges of GO were strongly related to pH. BET analysis showed the GO had a specific surface area of 30.7 m2/g and pore volume of 0.10 cm3/g. When the GO was used to remove the residual cellulase in bio-polishing effluent, it was found the removal capacity reached its maximum value at the pH 4-5...
November 27, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/29175288/mrcz-a-file-format-for-cryo-tem-data-with-fast-compression
#16
Robert A McLeod, Ricardo Diogo Righetto, Andy Stewart, Henning Stahlberg
The introduction of fast CMOS detectors is moving the field of transmission electron microscopy into the computer science field of big data. Automated data pipelines control the instrument and initial processing steps which imposes more onerous data transfer and archiving requirements. Here we conduct a technical demonstration whereby storage and read/write times are improved 10x at a dose rate of 1 e-/pix/frame for data from a Gatan K2 direct-detection device by combination of integer decimation and lossless compression...
November 23, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/29173812/activity-measurement-of-222rn-gas-for-a-key-comparison
#17
B J Kim, J M Lee, K B Lee, T S Park, B C Kim
The Korea Research Institute of Standards and Science (KRISS) participated, in 2015, in an international Key Comparison (KC) of gaseous radon-222 activity named CCRI (II) -K2.Rn-222 to confirm international equivalence of KRISS-established gaseous radon-222 measurement standards. LNHB acted as KC pilot laboratory. This paper describes the KC measurement procedure followed at KRISS using the defined solid angle counting method together with auxiliary relative measurement methods and presents the results.
November 7, 2017: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/29172023/investigations-of-the-local-distortions-and-epr-parameters-for-cu-2-in-xna2-o-30-x-k2-o-70b2-o3-5-%C3%A2-x-%C3%A2-25-mol-glasses
#18
Zhen-Ya Zhang, Shao-Yi Wu, Fu Zhang, Cheng-Xi Zhang, Rui-Jie Qin, Han Gao
The local distortions and electron paramagnetic resonance (EPR) parameters for Cu(2+) in the mixed alkali borate glasses xNa2 O-(30-x)K2 O-70B2 O3 (5 ≤ x ≤ 25 mol%) are theoretically studied with distinct modifier Na2 O compositions x. Owing to the Jahn-Teller effect, the octahedral [CuO6 ](10-) clusters show significant tetragonal elongation ratios p ~ 19% along the C4 axis. With the increase of composition x, the cubic field parameter Dq and the orbital reduction factor k exhibit linearly and quasi-linearly decreasing tendencies, respectively, while the relative tetragonal elongation ratio p has quasi-linearly increasing rule with some fluctuations, leading to the minima of g factors at x = 10 mol %...
November 24, 2017: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/29171854/preparation-and-characterization-of-terdentate-c-n-n-acetophenone-and-acetylpyridine-hydrazone-platinacycles-a-dft-insight-into-the-reaction-mechanism
#19
Ismael Marcos, Vicente Ojea, Digna Vázquez-García, Jesús J Fernández, Alberto Fernández, Margarita López-Torres, Jorge Lado, José M Vila
The reaction of N-ortho-chlorophenyl-substituted acetylpyridine hydrazones (a and d) with K2[PtCl4] (n-butanol/water, 100 °C) gave mononuclear complexes 1a and 1d with the ligands as [N,N] bidentate. In contrast, the reaction of N-phenyl or N-meta-chlorophenyl hydrazones (b and c, respectively) under analogous reaction conditions gave the cycloplatinated species 2b and 2c with the ligand as [C,N,N] terdentate. The treatment of the mononuclear complexes 1a and 1d with NaOAc (n-butanol, 100 °C) gave the corresponding cycloplatinated complexes 2a and 2d...
November 24, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29170577/terminal-ni-ii-oh-oh2-complexes-in-trigonal-bipyramidal-geometries-derived-from-h2o
#20
Nathanael Lau, Yohei Sano, Joseph W Ziller, A S Borovik
The preparation and characterization of two Ni(II) complexes are described, a terminal Ni(II)-OH complex with the tripodal ligand tris[(N)-tertbutylureaylato)-N-ethyl)]aminato ([H3buea](3-)) and a terminal Ni (II)-OH2 complex with the tripodal ligand N,N',N″-[2,2',2″-nitrilotris(ethane-2,1-diyl)]tris(2,4,6-trimethylbenzenesulfonamido) ([MST](3-)). For both complexes, the source of the -OH and -OH2 ligand is water. The salts K2[Ni(II)H3buea(OH)] and NMe4[Ni(II)MST(OH2)] were characterized using perpendicular-mode X-band electronic paramagnetic resonance, Fourier transform infrared, UV-visible spectroscopies, and its electrochemical properties were evaluated using cyclic voltammetry...
March 29, 2017: Polyhedron
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