Zhensheng Zhong, Can Zhang, Rui Liu, Jun He, Han Yang, Zijie Cheng, Tao Wang, Meng Shao, Shu Fang, Shengzhao Zhang, Hui Shi, Rufeng Xue, Huijuan Zou, Zeyu Ke, Zhiguo Zhang, Jinhua Zhou
Motility is one of the most critical features to evaluate sperm quality. As longitudinal rolling of human sperm has long been ignored until recently, its detailed dynamics and cellular biological mechanisms are still largely unknown. Here we report an optical-tweezers-based method to evaluate the chirality and frequency of sperm rotation. According to the intensity distribution patterns of off-focus micron-size particles, we established a method to judge the orientation of the sperm head along the optical axis in the optical trap...
2022: Frontiers in Bioengineering and Biotechnology