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https://www.readbyqxmd.com/read/28744638/counts-of-msc-in-the-bone-marrow-of-young-and-old-cba-mice-after-a-single-exposure-to-osteogenic-stimuli-curettage-bmp-2-injection-or-antigens-s-typhimurium-antigenic-complex-and-in-heterotopic-bone-marrow-transplants
#1
Yu F Gorskaya, A Sh Dzharullaeva, D S Onsina, V G Nesterenko
The efficiency of cloning and the content of multipotent stromal cells (MSC) in the femoral bone marrow of intact CBA mice was 1.5 times less in old mice (24-36 months) than in young ones (2-3 months). The concentration of osteogenic MSC was higher in old vs. young mice (42±3 vs. 22±2%, respectively). Changes in the total counts of MSC and concentrations of osteogenic MSC in response to osteogenic (curettage, BMP-2) and immunogenic stimuli (S. typhimurium antigenic complex) were similar in young and old mice in comparison with intact controls of respective age...
July 25, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28736908/bmp-7-ameliorates-cobalt-alloy-particle-induced-inflammation-by-suppressing-th17-responses
#2
Fengrong Chen, Ruisong Chen, Haoyuan Liu, Rupeng Sun, Jianming Huang, Zheyuan Huang, Guojian Jian
Metal wear debris has been shown to activate an aseptic osteolytic process that causes failure in total joint arthroplasty (TJA). This osteolysis is characterized by a proinflammatory, self-propagating immune response involving primarily macrophages, dendritic cells, and activated osteoclasts, as well as T cells and B cells. The human bone morphogenic protein (BMP)-7, on the other hand, was shown to promote osteoblast survival, and reversed the downregulation of anabolic Smad proteins and Runx2 following cobalt injury...
July 24, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28735764/bmp-2-7-heterodimer-strongly-induces-bone-regeneration-in-the-absence-of-increased-soft-tissue-inflammation
#3
Takashi Kaito, Tokimitsu Morimoto, Yuki Mori, Sadaaki Kanayama, Takahiro Makino, Shota Takenaka, Yusuke Sakai, Satoru Otsuru, Yoshichika Yoshioka, Hideki Yoshikawa
BACKGROUND CONTEXT: Bone morphogenetic protein (BMP)-2/7 heterodimer is a stronger inducer of bone regeneration than individual homodimers. However, clinical application of its potent bone induction ability may be hampered if its use is accompanied by excessive inflammatory reactions. PURPOSE: We sought to quantitatively evaluate bone induction and inflammatory reactions by BMP heterodimer and corresponding BMP homodimers using ultra-high resolution magnetic resonance imaging (MRI) and micro-computed tomography...
July 20, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28734792/genetic-factors-of-cervical-spondylotic-myelopathy-a-systemic-review
#4
REVIEW
Guohua Wang, Yong Cao, Tianding Wu, Chunyue Duan, Jianhuang Wu, Jianzhong Hu, Hongbin Lu
BACKGROUND: Cervical spondylotic myelopathy (CSM) is a degenerative disorder of the neck. Recent studies have reported the roles of single nucleotide polymorphisms and abnormal gene expression in the etiology and development of CSM. However, a systemic review of these findings is currently unavailable. METHODS: A systemic review of genetic factors of CSM was conducted through searching PubMed and EMbase databases. A total of 9 studies were included in this study, which included 8 genes: brain derived neurotrophic factor (BDNF), osteopontin (OPN), bone morphogenic protein (BMP) 4, collagen IX, vitamin D receptor (VDR), apolipoprotein E (ApoE), hypoxia-inducible factor α (HIF-1α), and cyclooxygenase 2 (COX-2)...
July 19, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/28730696/cd34-cells-seeded-in-collagen-scaffolds-promote-bone-formation-in-a-mouse-calvarial-defect-model
#5
Jens Hertweck, Ulrike Ritz, Hermann Götz, Patrick C Schottel, Pol Maria Rommens, Alexander Hofmann
Bone tissue engineering (BTE) holds promise for managing the clinical problem of large bone defects. However, clinical adoption of BTE is limited due to limited vascularization of constructs, which could be circumvented by pre-cultivation of osteogenic and endothelial derived cells in natural-based polymer scaffolds. However, until now not many studies compared the effect of mono- and cocultures pre-seeded in collagen before implantation. We utilized a mouse calvarial defect model and compared five groups of collagen scaffolds: a negative control of a collagen scaffold alone, a positive control treated with BMP-7, monocultures of either human osteoblasts (hOBs) or CD34+ cells, and a coculture of hOB and CD34(+) cells...
July 21, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28726579/the-immunomodulatory-role-of-bmp-2-on-macrophages-to-accelerate-osteogenesis
#6
Fei Wei, Yinghong Zhou, Jing Wang, Changsheng Liu, Yin Xiao
Bone morphogenetic protein-2 (BMP-2) is one of the most frequently used osteogenic factors for osteogenesis. Macrophages, acting as both immune cells and osteoclast precursors, have an indispensable functional input during multiple stages of bone healing. This study aims to investigate the immunoregulatory role of BMP-2 on macrophages and the subsequent effects on osteogenesis. In the subcutaneous implantation study, gelatin sponge incorporated with 20 μg/mL BMP-2 rendered significantly enhanced macrophage infiltration compared to the gelatin sponge control...
July 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28715143/repetitive-short-span-application-of-extracellular-calcium-is-osteopromotive-to-osteoprogenitor-cells
#7
Hyungjin Jung, Thomas Mbimba, Mustafa Unal, Ozan Akkus
Extracellular calcium ([Ca(2+) ]E ) concentration has been suggested to stimulate osteoblastic activity; thus, calcium can be used to enhance fracture healing. However, systemic administration of calcium at high dose levels may cause physiological problems such as hypercalcemia. Short span application of single or repetitive [Ca(2+) ]E stimulus may be suggested as a novel regimen to reduce such side effects. However, osteopromotive effect of such short term [Ca(2+) ]E stimulus on osteoprogenitor cells has not yet been evaluated yet...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28713017/intracellular-co-delivery-of-sr-ion-and-phenamil-drug-through-mesoporous-bioglass-nanocarriers-synergizes-bmp-signaling-and-tissue-mineralization
#8
Jung-Hwan Lee, Nandin Mandakhbayar, Ahmed El-Fiqi, Haw-Won Kim
Inducing differentiation and maturation of resident multipotent stem cells (MSCs) is an important strategy to regenerate hard tissues in mal-calcification conditions. Here we explore a co-delivery approach of therapeutic molecules comprised of ion and drug through a mesoporous bioglass nanoparticle (MBN) for this purpose. Recently, MBN has offered unique potential as a nanocarrier for hard tissues, in terms of high mesoporosity, bone bioactivity (and possibly degradability), tunable delivery of biomolecules, and ionic modification...
July 13, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28709740/effects-of-size-and-thermophilic-pre-hydrolysis-of-banana-peel-during-anaerobic-digestion-and-biomethanation-potential-of-key-tropical-fruit-wastes
#9
Mary Jesuyemi Odedina, Boonya Charnnok, Kanyarat Saritpongteeraka, Sumate Chaiprapat
Methane production potential of tropical fruit wastes, namely lady-finger banana peel, rambutan waste and longan waste were compared using BMP assay and stoichiometric modified Buswell and Mueller equation. Methane yields based on volatile solid (VS) were in the order of ground banana peel, chopped banana peel, chopped longan waste, and chopped rambutan waste (330.6, 268.3, 234.6 and 193.2 mLCH4/gVS) that corresponded to their calculated biodegradability. In continuous operations of banana peel digestion at feed concentrations based on total solid (TS) 1-2%, mesophilic single stage digester run at 20-day hydraulic retention time (20-day HRT) failed at 2%TS, but successfully recovered at 1...
July 11, 2017: Waste Management
https://www.readbyqxmd.com/read/28704415/c-elegans-sma-10-regulates-bmp-receptor-trafficking
#10
Ryan J Gleason, Mehul Vora, Ying Li, Nanci S Kane, Kelvin Liao, Richard W Padgett
Signal transduction of the conserved transforming growth factor-β (TGFβ) family signaling pathway functions through two distinct serine/threonine transmembrane receptors, the type I and type II receptors. Endocytosis orchestrates the assembly of signaling complexes by coordinating the entry of receptors with their downstream signaling mediators. Recently, we showed that the C. elegans type I bone morphogenetic protein (BMP) receptor SMA-6, part of the TGFβ family, is recycled through the retromer complex while the type II receptor, DAF-4 is recycled in a retromer-independent, ARF-6 dependent manner...
2017: PloS One
https://www.readbyqxmd.com/read/28704392/redifferentiation-of-aged-human-articular-chondrocytes-by-combining-bone-morphogenetic-protein-2-and-melanoma-inhibitory-activity-protein-in-3d-culture
#11
Stephan Payr, Brigitte Tichy, Clemens Atteneder, Marc Michel, Thomas Tiefenboeck, Nikolaus Lang, Sylvia Nuernberger, Stefan Hajdu, Elizabeth Rosado-Balmayor, Stefan Marlovits, Christian Albrecht
Melanoma inhibitory activity (MIA) affects the differentiation to hyaline cartilage and can inhibit the osteogenic potential of bone morphogenetic protein (BMP)-2 in mesenchymal stem cells (MSC). The aim of this study was to investigate if MIA also inhibits the osteogenic potential of BMP-2 in human articular chondrocytes during redifferentiation, which may lead to a higher grade of differentiation without calcification. HAC of four female patients (mean age: 73.75 ±6.98) were seeded into 3D culture for 28 days; after adding the recombinant proteins, four groups were formed (Control, BMP-2, MIA, BMP-2+MIA)...
2017: PloS One
https://www.readbyqxmd.com/read/28692113/analysing-the-bioactive-makeup-of-demineralised-dentine-matrix-on-bone-marrow-mesenchymal-stem-cells-for-enhanced-bone-repair
#12
S J Avery, L Sadaghiani, A J Sloan, R J Waddington
Dentine matrix has proposed roles for directing mineralised tissue repair in dentine and bone; however, the range of bioactive components in dentine and specific biological effects on bone-derived mesenchymal stem cells (MSCs) in humans are less well understood. The aims of this study were to further elucidate the biological response of MSCs to demineralised dentine matrix (DDM) in enhancing wound repair responses and ascertain key contributing components. Dentine was obtained from human teeth and DDM proteins solubilised with ethylenediaminetetraacetic acid (EDTA)...
July 10, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28688862/programmable-cells-of-monocytic-origin-as-a-source-of-osteochondroprogenitors-effect-of-growth-factors-on-osteogenic-differentiation
#13
Yahya Açil, Björn Möller, Jörg Wiltfang, Fred Fändrich, Hendrik Ungefroren
We have demonstrated previously that peripheral blood monocytes can be converted in vitro to a multipotent stem cell-like cell termed programmable cell of monocytic origin (PCMO) and subsequently into cells with chondrocyte-like phenotype. Here, we investigated whether PCMO could also be differentiated into osteoblast-like cells using growth factors with known osteoinductive potency. Following stimulation with BMP-2, BMP-7, IGF-1 or TGF-β1 for 7 and 14 days, PCMOs were analyzed for mRNA expression of collagen types I and V, alkaline phosphatase, osteocalcin, runt-related transcription factor-2 (Runx2) and Osterix (Osx) by quantitative RT-PCR (qPCR) and the levels of collagen I in culture supernatants by ELISA...
June 4, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/28684382/a-regulatory-role-of-androgen-in-ovarian-steroidogenesis-by-rat-granulosa-cells
#14
Toru Hasegawa, Yasuhiko Kamada, Takeshi Hosoya, Shiho Fujita, Yuki Nishiyama, Nahoko Iwata, Yuji Hiramatsu, Fumio Otsuka
Excess androgen and insulin-like growth factor (IGF)-I in the ovarian follicle has been suggested to be involved in the pathophysiology of polycystic ovary syndrome (PCOS). Here we investigated the impact of androgen and IGF-I on the regulatory mechanism of ovarian steroidogenesis using rat primary granulosa cells. It was revealed that androgen treatment with dihydrotestosterone (DHT) amplified progesterone synthesis in the presence of FSH and IGF-I, whereas it had no significant effect on estrogen synthesis by rat granulosa cells...
July 3, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28681971/growth-plate-expression-profiling-large-and-small-breed-dogs-provide-new-insights-in-endochondral-bone-formation
#15
Michelle Teunissen, Frank M Riemers, Dik van Leenen, Marian J A Groot Koerkamp, Björn P Meij, Jacqueline Alblas, Louis C Penning, Alberto Miranda-Bedate, Marianna A Tryfonidou
The difference in the adult height of mammals, and hence in endochondral bone formation, is not yet fully understood and may serve to identify targets for bone and cartilage regeneration. In line with this hypothesis, the intra-species disparity between the adult height of Great Danes and Miniature Poodles was investigated at a transcriptional level. Microarray analysis of the growth plate of 5 Great Danes and 5 Miniature Poodles revealed 2981 unique genes that were differentially expressed, including many genes with an unknown role in skeletal development...
July 6, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28667462/the-evaluation-of-cartilage-differentiations-using-transforming-growth-factor-beta3-alone-and-with-combination-of-bone-morphogenetic-protein-6-on-adult-stem-cells
#16
C C Ude, H C Chen, M Y Norhamdan, B M Azizi, B S Aminuddin, B H I Ruszymah
In our quest to standardize our formula for a clinical trial, transforming growth factor-beta3 (TGF-β3) alone and in combination with bone morphogenetic protein-6 (BMP-6) were evaluated for their effectiveness in cartilage differentiation. Bone Marrow Stem Cells (BMSCs) and Adipose Derived Stem Cells (ADSCs) were induced to chondrogenic lineage using two different media. Native chondrocytes served as positive control. ADSCs and BMSCs proved multipotency by tri-lineage differentiations. ADSC has significantly higher growth kinetics compare to Chondrocyte only p ≤ 0...
June 30, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28661587/endochondral-ossification-in-critical-sized-bone-defects-via-readily-implantable-scaffold-free-stem-cell-constructs
#17
Phuong N Dang, Samuel Herberg, Davood Varghai, Hooman Riazi, Daniel Varghai, Alexandra McMillan, Amad Awadallah, Lauren M Phillips, Oju Jeon, Minh K Nguyen, Neha Dwivedi, Xiaohua Yu, William L Murphy, Eben Alsberg
The growing socioeconomic burden of musculoskeletal injuries and limitations of current therapies have motivated tissue engineering approaches to generate functional tissues to aid in defect healing. A readily implantable scaffold-free system comprised of human bone marrow-derived mesenchymal stem cells embedded with bioactive microparticles capable of controlled delivery of transforming growth factor-beta 1 (TGF-β1) and bone morphogenetic protein-2 (BMP-2) was engineered to guide endochondral bone formation...
July 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28656306/99tc-mdp-induced-human-osteoblast-proliferation-differentiation-and-expression-of-osteoprotegerin
#18
Jie Chen, Youyu Lan, Yue He, Chengsong He, Fen Xu, Yugao Zhang, Yi Zhao, Yi Liu
The aim of the present study was to examine the influence of technetium methylenediphosphonate (99Tc-MDP) on the proliferation and differentiation of human osteoblasts. Human iliac cancellous bone was isolated and cultured with either 99Tc‑MDP, β fibroblast growth factor (as a positive control) or medium only (as a negative control). Proliferation was assessed by direct cell counting, CCK‑8 assay and bromodeoxyuridine staining. The cell cycle and rate of apoptosis was assessed by propidium iodide staining and flow cytometry...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28639437/ultrasound-assisted-green-economic-synthesis-of-hydroxyapatite-nanoparticles-using-eggshell-bio-waste-and-study-of-mechanical-and-biological-properties-for-orthopaedic-applications
#19
Vijay H Ingole, Kamal Hany Hussein, Anil A Kashale, Kalyani Ghule, Tomaž Vuherer, Vanja Kokol, Jia-Yaw Chang, Yong-Chien Ling, Aruna Vinchurkar, Hom N Dhakal, Anil V Ghule
Nanostructured hydroxyapatite (HAp) is most favorable candidate biomaterial for bone tissue engineering because of its bioactive and osteoconductive properties. Herein, we report for the first time ultrasound assisted facile and economic approach for the synthesis of nanocrystalline hydroxyapatite (Ca10 (PO4 )6 (OH)2 ) using recycled eggshell bio-waste referred as EHAp. The process involves the reaction of eggshell bio-waste as a source of calcium and ammonium dihydrogen orthophosphate as a phosphate source...
June 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28637038/synergic-impact-of-vascular-calcification-and-low-autonomic-tone-in-mortality-of-hemodialysis-patients
#20
Hwee-Yeong Ng, Shu-Kai Hsueh, Yueh-Ting Lee, Terry Ting-Yu Chiou, Pei-Chen Huang, Chien-Te Lee
BACKGROUND: Both cardiovascular calcification and autonomic dysfunction are frequently encountered in hemodialysis patients. We aimed to investigate the relationship between cardiovascular calcification and heart rate variability (HRV) and their influence on long-term outcome. METHODS: Seventy-eight hemodialysis patients underwent echocardiogram and radiography of the pelvis and hands to identify valvular and vascular calcification. HRV was evaluated using a commercial machine...
June 22, 2017: Nephron
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