Charlotte Deschamps, Sylvain Denis, Delphine Humbert, Nathalie Priymenko, Sandrine Chalancon, Jana De Bodt, Tom Van de Wiele, Ignacio Ipharraguerre, Inma Alvarez-Acero, Caroline Achard, Emmanuelle Apper, Stéphanie Blanquet-Diot
Differences in dog breed sizes are an important determinant of variations in digestive physiology, mainly related to the large intestine. In vitro gut models are increasingly used as alternatives to animal experiments for technical, cost, societal, and regulatory reasons. Up to now, only one in vitro model of the canine colon incorporates the dynamics of different canine gut regions, yet no adaptations exist to reproduce size-related digestive parameters. To address this limitation, we developed a new model of the canine colon, the CANIne Mucosal ARtificial COLon (CANIM-ARCOL), simulating main physiochemical (pH, transit time, anaerobiosis), nutritional (ileal effluent composition), and microbial (lumen and mucus-associated microbiota) parameters of this ecosystem and adapted to three dog sizes (i...
January 23, 2024: Applied Microbiology and Biotechnology