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https://www.readbyqxmd.com/read/27863925/number-and-function-of-circulating-endothelial-progenitor-cells-in-patients-with-primary-budd-chiari-syndrome
#1
Rui Huang, Qingqiao Zhang, Qianxin Huang, Maoheng Zu, Hao Xu, Lingyu Zeng
BACKGROUND AND AIM: Primary Budd-Chiari syndrome (BCS) is associated with vascular endothelial injury. Circulating endothelial progenitor cells (EPCs) provide an endogenous mechanism to repair endothelial injury. This study investigated the levels and functionality of EPCs in patients with primary BCS. METHODS: EPCs (CD34(+)/CD133(+)/KDR(+)) were quantified in 82 patients with primary BCS (inferior vena cava type: n=19; hepatic vein type: n=22; and mixed type: n=41), 10 cirrhosis controls (CC group) and 10 age-matched healthy controls (HC group), using flow cytometry...
November 15, 2016: Clinics and Research in Hepatology and Gastroenterology
https://www.readbyqxmd.com/read/27786558/increased-water-content-in-bacterial-cellulose-synthesized-under-rotating-magnetic-fields
#2
Karol Fijałkowski, Anna Żywicka, Radosław Drozd, Adam Feliks Junka, Dorota Peitler, Marian Kordas, Maciej Konopacki, Patrycja Szymczyk, Rafał Rakoczy
The current study describes properties of bacterial cellulose (BC) obtained from Komagataeibacter xylinus cultures exposed to the rotating magnetic field (RMF) of 50 Hz frequency and magnetic induction of 34 mT for controlled time during 6 days of cultivation. The experiments were carried out in the customized RMF exposure system adapted for biological studies. The obtained BC displayed an altered micro-structure, degree of porosity, and water-related parameters in comparison to the non-treated, control BC samples...
October 27, 2016: Electromagnetic Biology and Medicine
https://www.readbyqxmd.com/read/27779404/rotation-driven-microfluidic-disc-for-white-blood-cell-enumeration-using-magnetic-bead-aggregation
#3
Yiwen Ouyang, Jingyi Li, Doris M Haverstick, James P Landers
We recently defined a magnetic bead-based assay that exploited an agglutination-like response for DNA and applied it to DNA-containing cell enumeration using inexpensive benchtop hardware [ J. Am. Chem. Soc. 2012 , 134 ( 12 ), 5689 - 96 ]. Although cost-efficient, the open-well format assay required numerous manual steps, and the magnetic field actuation scheme was not readily adaptable for integration. Here, we demonstrate a low-cost (<$2 in-lab), higher-throughput "pinwheel assay" platform that relies on a combination of a disposable rotation-driven microdisc (RDM), and a simple bidirectional rotating magnetic field (bi-RMF)...
October 25, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27681765/evaluation-of-functional-marrow-irradiation-based-on-skeletal-marrow-composition-obtained-using-dual-energy-computed-tomography
#4
Taiki Magome, Jerry Froelich, Yutaka Takahashi, Luke Arentsen, Shernan Holtan, Michael R Verneris, Keenan Brown, Akihiro Haga, Keiichi Nakagawa, Jennifer L Holter Chakrabarty, Sebastian Giebel, Jeffrey Wong, Kathryn Dusenbery, Guy Storme, Susanta K Hui
PURPOSE: To develop an imaging method to characterize and map marrow composition in the entire skeletal system, and to simulate differential targeted marrow irradiation based on marrow composition. METHODS AND MATERIALS: Whole-body dual energy computed tomography (DECT) images of cadavers and leukemia patients were acquired, segmented to separate bone marrow components, namely, bone, red marrow (RM), and yellow marrow (YM). DECT-derived marrow fat fraction was validated using histology of lumbar vertebrae obtained from cadavers...
November 1, 2016: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/27577585/eleven-year-descriptive-analysis-of-closed-court-verdicts-on-medical-errors-in-spain-and-massachusetts
#5
Priscila Giraldo, Luke Sato, Jose M Martínez-Sánchez, Mercè Comas, Kathy Dwyer, Maria Sala, Xavier Castells
OBJECTIVES: To evaluate and compare the characteristics of court verdicts on medical errors allegedly harming patients in Spain and Massachusetts from 2002 to 2012. DESIGN, SETTING AND PARTICIPANTS: We reviewed 1041 closed court verdicts obtained from data on litigation in the Thomson Reuters Aranzadi Westlaw databases in Spain (Europe), and 370 closed court verdicts obtained from the Controlled Risk and Risk Management Foundation of Harvard Medical Institutions (CRICO/RMF) in Massachusetts (USA)...
2016: BMJ Open
https://www.readbyqxmd.com/read/27574849/rosae-multiflorae-fructus-hot-water-extract-inhibits-a-murine-allergic-asthma-via-the-suppression-of-th2-cytokine-production-and-histamine-release-from-mast-cells
#6
Chang Ho Song, Thi Tho Bui, Chun Hua Piao, Hee Soon Shin, Dong-Hwa Shon, Eui-Hyeog Han, Hyoung Tae Kim, Ok Hee Chai
Mast cell-mediated anaphylactic reactions are involved in many allergic diseases, including asthma and allergic rhinitis. In Korea, where it has been used as a traditional medicine, Rosae Multiflorae fructus (RMF) is known to have potent antioxidative, analgesic, and anti-inflammatory activities and to have no obvious acute toxicity. However, its specific effect on asthma is still unknown. In this study, we evaluated whether or not RMF hot water extracts (RMFW) could inhibit ovalbumin (OVA)-induced allergic asthma and evaluated compound 48/80-induced mast cell activation to elucidate the mechanisms of asthma inhibition by RMFW...
September 2016: Journal of Medicinal Food
https://www.readbyqxmd.com/read/27203864/biologically-optimized-helium-ion-plans-calculation-approach-and-its-in-vitro-validation
#7
A Mairani, I Dokic, G Magro, T Tessonnier, F Kamp, D J Carlson, M Ciocca, F Cerutti, P R Sala, A Ferrari, T T Böhlen, O Jäkel, K Parodi, J Debus, A Abdollahi, T Haberer
Treatment planning studies on the biological effect of raster-scanned helium ion beams should be performed, together with their experimental verification, before their clinical application at the Heidelberg Ion Beam Therapy Center (HIT). For this purpose, we introduce a novel calculation approach based on integrating data-driven biological models in our Monte Carlo treatment planning (MCTP) tool. Dealing with a mixed radiation field, the biological effect of the primary (4)He ion beams, of the secondary (3)He and (4)He (Z  =  2) fragments and of the produced protons, deuterons and tritons (Z  =  1) has to be taken into account...
June 7, 2016: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/27007305/salt-effects-on-functional-traits-in-model-and-in-economically-important-lotus-species
#8
P Uchiya, F J Escaray, D Bilenca, F Pieckenstain, O A Ruiz, A B Menéndez
A common stress on plants is NaCl-derived soil salinity. Genus Lotus comprises model and economically important species, which have been studied regarding physiological responses to salinity. Leaf area ratio (LAR), root length ratio (RLR) and their components, specific leaf area (SLA) and leaf mass fraction (LMF) and specific root length (SRL) and root mass fraction (RMF) might be affected by high soil salinity. We characterised L. tenuis, L. corniculatus, L. filicaulis, L. creticus, L. burtii and L. japonicus grown under different salt concentrations (0, 50, 100 and 150 mm NaCl) on the basis of SLA, LMF, SRL and RMF using PCA...
July 2016: Plant Biology
https://www.readbyqxmd.com/read/26765506/seedling-root-responses-to-soil-moisture-and-the-identification-of-a-belowground-trait-spectrum-across-three-growth-forms
#9
Julie E Larson, Jennifer L Funk
Root trait variation and plasticity could be key factors differentiating plant performance under drought. However, water manipulation and root measurements are rarely coupled empirically across growth forms to identify whether belowground strategies are generalizable across species. We measured seedling root traits across three moisture levels in 18 Mediterranean forbs, grasses, and woody species. Drought increased the root mass fraction (RMF) and decreased the relative proportion of thin roots (indicated by increased root diameters and decreased specific root length (SRL)), rates of root elongation and growth, plant nitrogen uptake, and plant growth...
May 2016: New Phytologist
https://www.readbyqxmd.com/read/26752481/clinical-outcome-following-radiofrequency-denervation-for-refractory-sacroiliac-joint-dysfunction-using-the-simplicity-iii-probe-a-12-month-retrospective-evaluation
#10
Dominic Hegarty
BACKGROUND: Sacroiliac joint syndrome (SIJ) is diagnosed in 10% to 25% of cases of lower back pain. The response to traditional radiofrequency (RF) denervation of the SIJ has being inconsistent. The Simplicity III RF probe (Neruotherm. Inc.) offers a novel treatment option. OBJECTIVE: To evaluate the long-term clinical outcome (12 months) refractory SIJ syndrome in terms of pain intensity and functional improvement. A 50% reduction in intensity pain intensity (VAS) at 12 months was deemed clinically significant...
January 2016: Pain Physician
https://www.readbyqxmd.com/read/26688548/structure-of-a-tcr-mimic-antibody-with-target-predicts-pharmacogenetics
#11
Niloufar Ataie, Jingyi Xiang, Neal Cheng, Elliott J Brea, Wenjie Lu, David A Scheinberg, Cheng Liu, Ho Leung Ng
Antibody therapies currently target only extracellular antigens. A strategy to recognize intracellular antigens is to target peptides presented by immune HLA receptors. ESK1 is a human, T-cell receptor (TCR)-mimic antibody that binds with subnanomolar affinity to the RMF peptide from the intracellular Wilms tumor oncoprotein WT1 in complex with HLA-A*02:01. ESK1 is therapeutically effective in mouse models of WT1(+) human cancers. TCR-based therapies have been presumed to be restricted to one HLA subtype. The mechanism for the specificity and high affinity of ESK1 is unknown...
January 16, 2016: Journal of Molecular Biology
https://www.readbyqxmd.com/read/26460998/fast-biological-modeling-for-voxel-based-heavy-ion-treatment-planning-using-the-mechanistic-repair-misrepair-fixation-model-and-nuclear-fragment-spectra
#12
COMPARATIVE STUDY
Florian Kamp, Gonzalo Cabal, Andrea Mairani, Katia Parodi, Jan J Wilkens, David J Carlson
PURPOSE: The physical and biological differences between heavy ions and photons have not been fully exploited and could improve treatment outcomes. In carbon ion therapy, treatment planning must account for physical properties, such as the absorbed dose and nuclear fragmentation, and for differences in the relative biological effectiveness (RBE) of ions compared with photons. We combined the mechanistic repair-misrepair-fixation (RMF) model with Monte Carlo-generated fragmentation spectra for biological optimization of carbon ion treatment plans...
November 1, 2015: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/26443727/modification-of-the-ribosome-and-the-translational-machinery-during-reduced-growth-due-to-environmental-stress
#13
Antón Vila-Sanjurjo
Escherichia coli strains normally used under laboratory conditions have been selected for maximum growth rates and require maximum translation efficiency. Recent studies have shed light on the structural and functional changes undergone by the translational machinery in E. coli during heat and cold shock and upon entry into stationary phase. In these situations both the composition and the partitioning of this machinery into the different pools of cellular ribosomes are modified. As a result, the translational capacity of the cell is dramatically altered...
September 2008: EcoSal Plus
https://www.readbyqxmd.com/read/26433486/high-resource-capture-and-use-efficiency-and-effective-antioxidant-protection-contribute-to-the-invasiveness-of-alnus-formosana-plants
#14
Shiliang Liu, Yiming Luo, Rongjie Yang, Chengxiang He, Qingsu Cheng, Jianjun Tao, Bo Ren, Maohua Wang, Mingdong Ma
To investigate the traits contributing to the invasiveness of Alnus formosana and the mechanisms underlying its invasiveness, we compared A. formosana with its native congener (Alnus cremastogyne) under three light treatments (13%, 56%, and 100%). The consistently higher plant height, total leaf area, light-saturated photosynthetic rate (A(max)), light saturation point (LSP), light compensation point (LCP), respiration efficiency (RE), and non-photochemical quenching coefficient (NPQ) but lower root mass fraction (RMF) and specific leaf area (SLA) of the invader than of its native congener contributed to the higher RGR and total biomass of A...
November 2015: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/26425058/regional-myocutaneous-flaps-for-head-and-neck-reconstruction-experience-of-a-head-and-neck-cancer-unit
#15
Eyituoyo Okoturo
BACKGROUND: Pectoralis major myocutaneous pedicle flap and other regional myocutaneous pedicle flaps (RMF), despite the superiority shown by free flaps, have remained relevant in the reconstruction of major head and neck oncologic defects. It has continued to find relevance as the preferred reconstruction of choice in some general head and neck reconstructive applications. While its role has been defined in developed environment, the same cannot be said for developing environment. The aim thus, was to review our experiences with RMFs in head and neck reconstructions, with a view to evaluating the indications and outcomes in a limited opportunity environment with some free flaps expertise...
July 2015: Nigerian Journal of Surgery: Official Publication of the Nigerian Surgical Research Society
https://www.readbyqxmd.com/read/26344254/modification-of-bacterial-cellulose-through-exposure-to-the-rotating-magnetic-field
#16
Karol Fijałkowski, Anna Żywicka, Radosław Drozd, Agata Niemczyk, Adam Feliks Junka, Dorota Peitler, Marian Kordas, Maciej Konopacki, Patrycja Szymczyk, Mirosława El Fray, Rafał Rakoczy
The aim of the study was to assess the influence of rotating magnetic field (RMF) on production rate and quality parameters of bacterial cellulose synthetized by Glucanacetobacter xylinus. Bacterial cultures were exposed to RMF (frequency f=50Hz, magnetic induction B=34mT) for 72h at 28°C. The study revealed that cellulose obtained under RMF influence displayed higher water absorption, lower density and less interassociated microfibrils comparing to unexposed control. The application of RMF significantly increased the amount of obtained wet cellulose pellicles but decreased the weight and thickness of dry cellulose...
November 20, 2015: Carbohydrate Polymers
https://www.readbyqxmd.com/read/26324267/ribosome-hibernation-facilitates-tolerance-of-stationary-phase-bacteria-to-aminoglycosides
#17
Susannah L McKay, Daniel A Portnoy
Upon entry into stationary phase, bacteria dimerize 70S ribosomes into translationally inactive 100S particles by a process called ribosome hibernation. Previously, we reported that the hibernation-promoting factor (HPF) of Listeria monocytogenes is required for 100S particle formation and facilitates adaptation to a number of stresses. Here, we demonstrate that HPF is required for the high tolerance of stationary-phase cultures to aminoglycosides but not to beta-lactam or quinolone antibiotics. The sensitivity of a Δhpf mutant to gentamicin was suppressed by the bacteriostatic antibiotics chloramphenicol and rifampin, which inhibit translation and transcription, respectively...
November 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26290418/role-of-100s-ribosomes-in-bacterial-decay-period
#18
Ksenia Shcherbakova, Hideki Nakayama, Nobuo Shimamoto
Ribosomal proteins S10 and S2 were each fused with GFP to track the fates of these proteins in the stationary growth phase and the following decay period in Escherichia coli. The fused proteins localized mainly in the cytoplasm, and their amounts were proportional to the colony-forming unit. S10-GFP strains that lacked genes responsible for regulating 100S ribosomes and S2-GFP strain that was unable to form 100S both showed shortened stationary phases. This result indicates that these strains exhibit earlier death in the absence of 100S formation (S2-GFP, S10-GFP∆rmf and S10-GFP∆hpf) and breakdown (S10-GFP∆yfiA)...
October 2015: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/26241612/the-acute-effect-of-concurrent-training-on-running-performance-over-6-days
#19
Kenji Doma, Glen Deakin
PURPOSE: This study examined the effects of strength training on alternating days and endurance training on consecutive days on running performance for 6 days. METHODS: Sixteen male and 8 female moderately trained individuals were evenly assigned into concurrent-training (CCT) and strength-training (ST) groups. The CCT group undertook strength training on alternating days combined with endurance training on consecutive days for 6 days. One week later, the CCT group conducted 3 consecutive days of endurance training only to determine whether fatigue would be induced with endurance training alone (CCT-Con)...
2015: Research Quarterly for Exercise and Sport
https://www.readbyqxmd.com/read/26177672/evidence-supporting-see-and-treat-management-of-cervical-intraepithelial-neoplasia-a-systematic-review-and-meta-analysis
#20
REVIEW
R M F Ebisch, M M Rovers, R P Bosgraaf, H W van der Pluijm-Schouten, W J G Melchers, P A J van den Akker, L F A G Massuger, R L M Bekkers
BACKGROUND: Studies of see-and-treat management of cervical intraepithelial neoplasia (CIN) vary in their inclusion criteria, resulting in a broad range of overtreatment rates. OBJECTIVES: To determine overtreatment rates in see-and-treat management of women referred for colposcopy because of suspected CIN, in order to define circumstances supporting see-and-treat management. SEARCH STRATEGY: MEDLINE, EMBASE, and the Cochrane Library were searched from inception up to 12 May 2014...
January 2016: BJOG: An International Journal of Obstetrics and Gynaecology
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