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https://www.readbyqxmd.com/read/29089333/electrical-pulse-stimulation-induces-glut4-glucose-transporter-translocation-in-c2c12-myotubes-that-depends-on-rab8a-rab13-and-rab14
#1
Zhu Li, Yingying Yue, Fang Hu, Chang Zhang, Xiaofang Ma, Nana Li, Lihong Qiu, Maolong Fu, Liming Chen, Zhi Yao, Philip J Bilan, Amira Klip, Wenyan Niu
The signals mobilizing GLUT4 to the plasma membrane in response to muscle contraction are less known than those elicited by insulin. This disparity is undoubtedly due to lack of suitable in vitro models to study skeletal muscle contraction. We generated C2C12 myotubes stably expressing HA-tagged GLUT4 (C2C12-GLUT4HA) that contract in response to electrical pulse stimulation (EPS) and investigated molecular mechanisms regulating GLUT4HA EPS (60min, 20V, 1Hz, 24ms pulses at 976ms intervals) elicited a gain in surface GLUT4HA (GLUT4 translocation) comparably to insulin or 5-amino imidazole-4-carboxamide ribonucleotide (AICAR)...
October 31, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29045420/inhibition-of-apoptosis-signal-regulating-kinase-1-alters-the-wound-epidermis-and-enhances-auricular-cartilage-regeneration
#2
Qian-Shi Zhang, Deepa S Kurpad, My G Mahoney, Marla J Steinbeck, Theresa A Freeman
Why regeneration does not occur in mammals remains elusive. In lower vertebrates, epimorphic regeneration of the limb is directed by the wound epidermis, which controls blastema formation to promote regrowth of the appendage. Herein, we report that knockout (KO) or inhibition of Apoptosis Signal-regulated Kinase-1 (ASK1), also known as mitogen-activated protein kinase kinase kinase 5 (MAP3K5), after full thickness ear punch in mice prolongs keratinocyte activation within the wound epidermis and promotes regeneration of auricular cartilage...
2017: PloS One
https://www.readbyqxmd.com/read/28990082/fluid-shear-stress-induces-osteoblast-differentiation-and-arrests-the-cell-cycle-at-the-g0-phase-via-the-erk1-2-pathway
#3
Liyin Yu, Xingfeng Ma, Junqin Sun, Jie Tong, Liang Shi, Lijun Sun, Jianbao Zhang
Numerous studies have demonstrated that fluid shear stress (FSS) may promote the proliferation and differentiation of osteoblast cells. However, proliferation and differentiation are mutually exclusive processes and are unlikely to be promoted by FSS simultaneously. Cell proliferation and differentiation induced by FSS has rarely been reported. In order to provide an insight into this process, the present study investigated the effects of FSS on osteoblast‑like MC3T3 cells in the G0/G1 phase, the period during which the fate of a cell is determined...
December 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28970726/ca-2-calmodulin-dependent-protein-kinase-ii-regulates-colon-cancer-proliferation-and-migration-via-erk1-2-and-p38-pathways
#4
Wei Chen, Ping An, Xiao-Jing Quan, Jun Zhang, Zhong-Yin Zhou, Li-Ping Zou, He-Sheng Luo
AIM: To investigate the role of calmodulin-dependent protein kinase II (CaMKII) in colon cancer growth, migration and invasion. METHODS: CaMKII expression in colon cancer and paracancerous tissues was evaluated via immunochemistry. Transcriptional and posttranscriptional levels of CaMKIIin tissue samples and MMP2, MMP9 and TIMP-1 expression in the human colon cancer cell line HCT116 were assessed by qRT-PCR and western blot. Cell proliferation was detected with the MTT assay...
September 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28937250/role-of-exercise-induced-calmodulin-protein-kinase-camk-ii-activation-in-the-regulation-of-omega-6-fatty-acids-and-lipid-metabolism-genes-in-rat-skeletal-muscle
#5
J S Joseph, A O Ayeleso, E Mukwevho
Activation of calmodulin dependent protein kinase (CaMK)II by exercise is beneficial in controlling membrane lipids associated with type 2 diabetes and obesity. Regulation of lipid metabolism is crucial in the improvement of type 2 diabetes and obesity associated symptoms. The role of CaMKII in membrane associated lipid metabolism was the focus of this study. Five to six weeks old male Wistar rats were used in this study. GC×GC-TOFMS technique was used to determine the levels of polyunsaturated fatty acids (linoleic acid, arachidonic acid and 11,14-eicosadienoic acid)...
September 22, 2017: Physiological Research
https://www.readbyqxmd.com/read/28805296/basidiomycete-specific-pscamkl1-encoding-a-camk-like-protein-kinase-is-required-for-full-virulence-of-puccinia-striiformis-f-sp-tritici
#6
Min Jiao, Dan Yu, Chenglong Tan, Jia Guo, Dingyun Lan, Ershang Han, Tuo Qi, Ralf Thomas Voegele, Zhensheng Kang, Jun Guo
Calcium/calmodulin-dependent kinases (CaMKs) are Ser/Thr protein kinases (PKs) that respond to changes in cytosolic free Ca(2+) and play diverse roles in eukaryotes. In fungi, CAMKs are generally classified into four families CAMK1, CAMKL, RAD53 and CAMK-Unique. Among these, CAMKL constitutes the largest family. In some fungal plant pathogens, members of the CaMKL family have been shown to be responsible for pathogenesis. However, little is known about their role(s) in rust fungi. In this study, we functionally characterized a novel PK gene, PsCaMKL1, from Puccinia striiformis f...
August 14, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28768289/%C3%AE-adrenergic-receptor-stimulated-cardiac-myocyte-apoptosis-role-of-cytochrome-p450-%C3%AF-hydroxylase
#7
Shan Jiang, Dan Huo, Xueying Wang, Huan Zhao, Jiang Tan, Qinghua Zeng, Stephen T OʼRourke, Chengwen Sun
Prolonged or excessive β-adrenergic activation leads to cardiac myocyte loss and heart dysfunction; however, the underlying cellular mechanisms are still unclear. Therefore, we first confirmed the effect of isoproterenol (ISO), a β-adrenergic receptor agonist, on cardiac toxicity using TUNEL and caspase activity assays in cultured rat cardiomyocytes. ISO treatment significantly increased cardiomyocyte apoptosis. Persistent ISO stimulation of cardiomyocytes also increased the expression of CYP4A3, a major CYP450 ω-hydroxylase that produces 20-hydroxyeicosatetraenoic acid (20-HETE) in a time-dependent manner...
August 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28668302/role-of-camkii-in-free-fatty-acid-hyperlipidemia-induced-cardiac-remodeling-both-in-vitro-and-in-vivo
#8
Peng Zhong, Dajun Quan, Jianye Peng, Xiaoju Xiong, Yu Liu, Bin Kong, He Huang
RATIONALE: The cellular mechanisms of obesity/hyperlipidemia-induced cardiac remodeling are many and not completely elucidated. Ca(2+)/calmodulin-dependent protein kinase II (CaMKII), a multifunctional serine/threonine kinase, has been reported to be involved in a variety of cardiovascular diseases. However, its role in obesity/hyperlipidemia-induced cardiac remodeling is still unknown. OBJECTIVE: The objective of this study was to demonstrate the role of CaMKII in the pathogenesis of obesity/hyperlipidemia-induced cardiac remodeling both in vitro and in vivo...
August 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28662005/camkii-activation-promotes-cardiac-electrical-remodeling-and-increases-the-susceptibility-to-arrhythmia-induction-in-high-fat-diet-fed-mice-with-hyperlipidemia-conditions
#9
Peng Zhong, Dajun Quan, Yan Huang, He Huang
BACKGROUND: Obesity/hyperlipidemia is closely related to both atrial and ventricular arrhythmias. CaMKII, a multifunctional serine/threonine kinase, has been involved in cardiac arrhythmias of different etiologies. However, its role in obesity/hyperlipidemia-related cardiac arrhythmia is unexplored. The aim of this was to determine the involvement of CaMKII in the process. METHODS: Adult male APOE mice were fed a high-fat diet (HFD), administrated with KN93 (10 mg·kg·2d), a specific inhibitor of CaMKII...
October 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28597397/protein-kinase-c%C3%AE-gene-depletion-protects-against-methamphetamine-induced-impairments-in-recognition-memory-and-erk1-2-signaling-via-upregulation-of-glutathione-peroxidase-1-gene
#10
The-Vinh Tran, Eun-Joo Shin, Lan Thuy Ty Nguyen, Youngho Lee, Dae-Joong Kim, Ji Hoon Jeong, Choon-Gon Jang, Seung-Yeol Nah, Kazuya Toriumi, Toshitaka Nabeshima, Kiyofumi Yamada, Hyoung-Chun Kim
Accumulating evidence has suggested that repeated treatment with methamphetamine (MA) resulted in cognitive impairments. Importantly, we show that selective upregulation of protein kinase Cδ (PKCδ) in the prefrontal cortex (PFC) of wild-type mice persisted for 28 days post withdrawal of MA. On day 28, the MA-induced increase in phospho-PKCδ expression and decrease in phospho-ERK1/2 expression were significantly attenuated by both the Src inhibitor PP2 and the dopamine D1 receptor antagonist SCH 23390. However, neither protein kinase A inhibitor H89 nor calmodulin-dependent protein kinase II inhibitor KN93 attenuated MA-induced alterations in phospho-PKCδ expression and phospho-ERK1/2 expression...
June 9, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28566491/chronic-hypoxia-alters-fetal-cerebrovascular-responses-to-endothelin-1
#11
Jinjutha Silpanisong, Dahlim Kim, James M Williams, Olayemi O Adeoye, Richard B Thorpe, William J Pearce
In utero hypoxia influences the structure and function of most fetal arteries, including those of the developing cerebral circulation. Whereas the signals that initiate this hypoxic remodeling remain uncertain, these appear to be distinct from the mechanisms that maintain the remodeled vascular state. The present study explores the hypothesis that chronic hypoxia elicits sustained changes in fetal cerebrovascular reactivity to endothelin-1 (ET-1), a potent vascular contractant and mitogen. In fetal lambs, chronic hypoxia (3,820-m altitude for the last 110 days of gestation) had no significant effect on plasma ET-1 levels or ETA receptor density in cerebral arteries but enhanced contractile responses to ET-1 in an ETA-dependent manner...
August 1, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28489932/camkii%C3%AE-may-modulate-fentanyl-induced-hyperalgesia-via-a-celc-pag-rvm-spinal-cord-descending-facilitative-pain-pathway-in-rats
#12
Zhen Li, Pingping Yin, Jian Chen, Shenglan Jin, Jieqiong Liu, Fang Luo
Each of the lateral capsular division of central nucleus of amygdala(CeLC), periaqueductal gray (PAG), rostral ventromedial medulla(RVM) and spinal cord has been proved to contribute to the development of opioid-induced hyperalgesia(OIH). Especially, Ca2+/calmodulin-dependent protein kinase IIα (CaMKIIα) in CeLC and spinal cord seems to play a key role in OIH modulation. However, the pain pathway through which CaMKIIα modulates OIH is not clear. The pathway from CeLC to spinal cord for this modulation was explored in the present study...
2017: PloS One
https://www.readbyqxmd.com/read/28263745/exercise-increases-hyper-acetylation-of-histones-on-the-cis-element-of-nrf-1-binding-to-the-mef2a-promoter-implications-on-type-2-diabetes
#13
Jitcy S Joseph, Ademola O Ayeleso, Emmanuel Mukwevho
Exercise brings changes on the chromatin ensuing the upregulation of many genes that confer protection from type 2 diabetes. In type-2 diabetes, critical genes are down-regulated such as those involved in glucose transport (GLUT4, MEF2A) and also oxidative phosphorylation (NRF-1 and its target genes). Recent reports have shown that NRF-1 not only regulate mitochondrial oxidative genes but also controls MEF2A, the main transcription factor for glucose transporter, GLUT4. Such dual control of the two pathways by NRF-1 place it as critical gene in the design of therapeutic modalities much needed to cure or better manage type 2 diabetes...
April 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28241801/the-protective-effects-of-propofol-against-cocl2-induced-ht22-cell-hypoxia-injury-via-pp2a-camkii%C3%AE-nnos-pathway
#14
Yan Lu, Wei Chen, Chen Lin, Jiaqiang Wang, Minmin Zhu, Jiawei Chen, Changhong Miao
BACKGROUND: Perioperative cerebral ischemia/hypoxia could induce hippocampal injury and has been reported to induce cognitive impairment. In this study, we used cobalt chloride (CoCl2) to build a hypoxia model in mouse hippocampal cell lines. Propofol, a widely used intravenous anesthetic agent, has been demonstrated to have neuroprotective effect. Here, we explored whether and how propofol attenuated CoCl2-induced mouse hippocampal HT22 cell injury. METHODS: Mouse hippocampal HT22 cells were pretreated with propofol, and then stimulated with CoCl2...
February 28, 2017: BMC Anesthesiology
https://www.readbyqxmd.com/read/28230177/storage-of-passive-motion-pattern-in-hippocampal-ca1-region-depends-on-camkii-creb-signaling-pathway-in-a-motion-sickness-rodent-model
#15
Junqin Wang, Jiluo Liu, Leilei Pan, Ruirui Qi, Peng Liu, Wei Zhou, Yiling Cai
Sensory mismatch between actual motion information and anticipated sensory patterns (internal model) is the etiology of motion sickness (MS). Some evidence supports that hippocampus might involve the neural storage of the "internal model". This study established an "internal model" acquisition-retention behavioral model using a repeated habituation rotation training protocol. We tried to identify the hippocampal subregion involved in "internal model" retention using chemical lesion methods. Hippocampal kinases (CaMK, CaMKIV, CREB and ERK1/2) phosphorylation in the target subregion was assayed and the effects of kinase inhibitors (KN93 or U0126) on "internal model" retention were investigated...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28188886/propofol-inhibits-high-glucose-induced-pp2a-expression-in-human-umbilical-vein-endothelial-cells
#16
Qichao Wu, Yanjun Zhao, Wenming Duan, Yi Liu, Xiangyuan Chen, Minmin Zhu
Perioperative hyperglycemia is a common clinical metabolic disorder. Hyperglycemia could induce endothelial apoptosis, dysfunction and inflammation, resulting in endothelial injury. Propofol is a widely used anesthetic drug in clinical settings. Our previous studies indicated that propofol, via inhibiting high glucose-induced phosphatase A2 (PP2A) expression, attenuated high glucose-induced reactive oxygen species (ROS) accumulation, thus improving endothelial apoptosis, dysfunction and inflammation. However, the mechanisms by which propofol attenuated high glucose-induced PP2A expression is still obscure...
April 2017: Vascular Pharmacology
https://www.readbyqxmd.com/read/28181390/tris-1-3-dichloro-2-propyl-phosphate-induces-toxicity-by-stimulating-camk2-in-pc12-cells
#17
Chaonan Li, Li Li, Bencheng Lin, Yanjun Fang, Honglian Yang, Huanliang Liu, Zhuge Xi
Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is one of the widely used organophosphorus flame retardants (OPFRs), which are regarded as suitable substitutes for brominated flame retardants (BFRs). Previously, we have validated the toxicity of TDCIPP in PC12 cells owing to the induced alterations in GAP43, NF-H, CaMK2a/2b, and tubulin α/β proteins; however, limited information is currently available on the toxicity and mechanism of TDCIPP. In the present study, cytotoxicity effects were evaluated by exposing PC12 cells to different concentrations of TDCIPP (0-50 μM) for 4 days...
June 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28122728/absence-of-suppressor-of-cytokine-signaling-2-turns-cardiomyocytes-unresponsive-to-lif-dependent-increases-in-ca-2-levels
#18
Cibele Rocha-Resende, Itamar Couto Guedes de Jesus, Danilo Roman-Campos, Artur S Miranda, Fabiana Alves, Rodrigo Ribeiro Resende, Jader Dos Santos Cruz, Fabiana Simão Machado, Silvia Guatimosim
Little is known regarding the role of suppressor of cytokine signaling (SOCS) in the control of cytokine signaling in cardiomyocytes. We investigated the consequences of SOCS2 ablation for leukemia inhibitory factor (LIF)-induced enhancement of intracellular Ca(2+) ([Ca(2+)]i) transient by performing experiments with cardiomyocytes from SOCS2-knockout (ko) mice. Similar levels of SOCS3 transcripts were seen in cardiomyocytes from wild-type and SOCS2-ko mice, while SOCS1 mRNA was reduced in SOCS2-ko. Immunoprecipitation experiments showed increased SOCS3 association with gp130 receptor in SOCS2-ko myocytes...
April 1, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28117139/epigallocatechin-3-gallate-modulates-arrhythmogenic-activity-and-calcium-homeostasis-of-left-atrium
#19
Jun-Hei Chang, Shih-Lin Chang, Po-Da Hong, Ping-Nan Chen, Chih-Hsueng Hsu, Yen-Yu Lu, Yao-Chang Chen
BACKGROUND: Atrial fibrillation (AF) is the commonest sustained arrhythmia, and increases the risk of stroke, heart failure, and mortality. Calcium (Ca(2+)) overload and oxidative stress are thought to participate in the pathogenesis of AF. Epigallocatechin-3-gallate (EGCG) has an antioxidative effect and been shown to be beneficial in promoting cardiovascular health. However, it is not clear if EGCG directly modulates the electrophysiological characteristics and Ca(2+) homeostasis of the left atrium (LA)...
January 14, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/27986626/propofol-attenuates-pancreatic-cancer-malignant-potential-via-inhibition-of-nmda-receptor
#20
Xiangyuan Chen, Qichao Wu, Li You, Sisi Chen, Minmin Zhu, Changhong Miao
Propofol is a commonly used intravenous anesthetic, and could attenuate cancer cells malignant potential via inhibiting hypoxia-inducible factor-1α (HIF-1α) expression. However, the mechanism is still inclusive. In the present study, we mainly focus on the mechanism by which propofol down-regulated HIF-1α expression and malignant potential in pancreatic cancer cells. Human pancreatic cancer cells (Miapaca-2 and Panc-1) in vitro and murine pancreatic cancer cell (Panc02) in vivo were used to assess the effect of propofol on vascular endothelial growth factor (VEGF) expression and migration of pancreatic cancer cells...
January 15, 2017: European Journal of Pharmacology
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