Ying Wang, Weiwei Liang, Tianhang Wang, Chunting Zhang, Yueqing Yang, Chaohua Cong, Xudong Wang, Shuyu Wang, Di Wang, Di Huo, Hongyong Wang, Xiaoli Su, Xingli Tan, Honglin Feng
BACKGROUND: ANO1 is closely correlated with the activation of EGFR and CaMKII, while EGFR and CaMKII show low activation in amyotrophic lateral sclerosis (ALS) models. Therefore, we designed experiments to verify that ANO1 may play a protective role on motor neurons in ALS by activating EGFR and CaMKII. METHODS: The expression changes of ANO1, EGFR, CaMKII, pEGFR, and pCaMKII, cell survival status, and apoptosis were studied by western blot, real-time quantitative PCR, immunofluorescence, immunohistochemistry, CCK-8, and flow cytometry...
October 17, 2023: Brain Research Bulletin