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okadaic acid

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https://www.readbyqxmd.com/read/28439489/detection-and-analysis-of-lactobacillus-paracasei-penicillin-binding-proteins-revealed-the-presence-of-cholate-sensitive-penicillin-binding-protein-3-and-an-elongated-cell-shape-in-a-cholate-sensitive-strain
#1
Masahiro Hattori, Glaezel Angelique Torres, Naoto Tanaka, Sanae Okada, Akihito Endo, Junichi Nakagawa
Penicillin-binding proteins (PBPs) are responsible for peptidoglycan synthesis. By using biotinylated ampicillin, we detected PBPs of Lactobacillus paracasei strains. Ten PBPs were identified, 7 of which had apparent molecular sizes similar to those of Escherichia coli. In the presence of cholate, strain NRIC 0625 showed an elongated shape, and its putative PBP3 showed cholate-sensitive penicillin-binding activity. Furthermore, this strain was highly sensitive to cefalexin, which is known to inhibit cell division by inactivating PBP3...
2017: Bioscience of Microbiota, Food and Health
https://www.readbyqxmd.com/read/28439300/genetic-variants-of-helicobacter-pylori-type-iv-secretion-system-components-cagl-and-cagi-and-their-association-with-clinical-outcomes
#2
Hirofumi Ogawa, Akira Iwamoto, Toshihito Tanahashi, Rina Okada, Koji Yamamoto, Shin Nishiumi, Masaru Yoshida, Takeshi Azuma
BACKGROUND: Helicobacter pylori infection is associated with risk for chronic gastritis (CG), gastric ulcer (GU), duodenal ulcer (DU), and gastric cancer (GC). The H. pylori Cag type IV secretion system (TFSS) translocates the virulence factor cytotoxin-associated gene A protein into host cells and plays an important role in initiating gastric carcinogenesis. The CagL and CagI proteins are components of the TFSS. The Arg-Gly-Asp (RGD) motif of CagL, and the six most distal C-terminal amino acids (Ser-Lys-Ile-Ile-Val-Lys, and Ser-Lys-Val-Ile-Val-Lys) of CagL and CagI are essential for TFSS adhesion to host cells...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28429183/label-free-okadaic-acid-detection-using-growth-of-gold-nanoparticles-in-sensor-gaps-as-a-conductive-tag
#3
Yuxiang Pan, Zijian Wan, Longjie Zhong, Xueqin Li, Qi Wu, Jun Wang, Ping Wang
Okadaic acid (OA) is a marine toxin ingested by shellfish. In this work, a simple, sensitive and label-free gap-based electrical competitive bioassay has been developed for this biotoxin detection. The gap-electrical biosensor is constructed by modifying interdigitated microelectrodes with gold nanoparticles (AuNPs) and using the self-catalytic growth of AuNPs as conductive bridges. In this development, the AuNPs growth is realized in the solution of glucose and chloroauric acid, with glucose oxidation used as the catalysis for growth of the AuNPs...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28427570/high-resolution-mass-spectrometry-based-screening-reveals-lipophilic-toxins-in-multiple-trophic-levels-from-the-north-sea
#4
Gabriel Orellana, Lieven Van Meulebroek, Maarten De Rijcke, Colin R Janssen, Lynn Vanhaecke
Lipophilic marine biotoxins, which are mainly produced by small dinoflagellates, are increasingly detected in coastal waters across the globe. As these producers are consumed by zooplankton and shellfish, the toxins are introduced, bioaccumulated and possibly biomagnified throughout marine food chains. Recent research has demonstrated that ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS) is an excellent tool to detect marine toxins in algae and seafood. In this study, UHPLC-HRMS was used to screen lipophilic marine biotoxins in organisms from different trophic levels of the Belgian coastal zone ecosystem...
April 2017: Harmful Algae
https://www.readbyqxmd.com/read/28420890/endoscopic-manifestations-and-clinical-characteristics-of-cytomegalovirus-infection-in-the-upper-gastrointestinal-tract
#5
Masaya Iwamuro, Eisei Kondo, Takehiro Tanaka, Hideharu Hagiya, Seiji Kawano, Yoshiro Kawahara, Fumio Otsuka, Hiroyuki Okada
We retrospectively analyzed the cases of 14 patients (9 women, 5 men, mean age: 51.6 years) with cytomegalovirus (CMV) involvement in the esophagus, stomach, and/or duodenum diagnosed at a single center, to determine their endoscopic features and clinical backgrounds. Thirteen patients (92.9%) had hematologic disease; the other had rheumatoid arthritis. Of the former, 12 patients underwent allogeneic hematopoietic stem cell transplantation, and 9 of these patients had graft-versus-host disease (GVHD) before undergoing esophagogastroduodenoscopy (EGD)...
April 2017: Acta Medica Okayama
https://www.readbyqxmd.com/read/28420813/establishment-of-cell-lines-from-adult-t-cell-leukemia-cells-dependent-on-negatively-charged-polymers
#6
Yoshitoyo Kagami, Susumu Uchiyama, Harumi Kato, Yasutaka Okada, Masao Seto, Tomohiro Kinoshita
Growing adult T-cell leukemia/lymphoma (ATLL) cells in vitro is difficult. Here, we examined the effects of static electricity in the culture medium on the proliferation of ATLL cells. Six out of 10 ATLL cells did not proliferate in vitro and thus had to be cultured in a medium containing negatively charged polymers. In the presence of poly-γ-glutamic acid (PGA) or chondroitin sulfate (CDR), cell lines (HKOX3-PGA, HKOX3-CDR) were established from the same single ATLL case using interleukin (IL)-2, IL-4, and feeder cells expressing OX40L (OX40L(+)HK)...
April 18, 2017: Journal of Clinical and Experimental Hematopathology: JCEH
https://www.readbyqxmd.com/read/28419442/characterization-of-runx2-phosphorylation-sites-required-for-tgf-%C3%AE-1-mediated-stimulation-of-matrix-metalloproteinase-13-expression-in-osteoblastic-cells
#7
B Arumugam, M Vairamani, N C Partridge, N Selvamurugan
Transforming growth factor-beta1 (TGF-β1), a highly abundant growth factor in skeletal tissues, stimulates matrix metalloproteinase-13 (MMP-13) expression in osteoblastic cells. MMP-13 plays a critical role in bone remodeling. Runx2, a bone transcription factor, is required for TGF-β1-mediated stimulation of MMP-13 expression in osteoblastic cells. In this study, the molecular mechanism responsible for TGF-β1-stimulation of MMP-13 expression via Runx2 in osteoblastic cells was elucidated. TGF-β1 stimulated the phosphorylation of Runx2 at serine amino acids, and ERK inhibition blocked this effect in rat (UMR106-01) and human (MG-63) osteoblastic cells...
April 17, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28414161/metabolism-of-okadaic-acid-by-nadph-dependent-enzymes-present-in-human-or-rat-liver-s9-fractions-results-in-different-toxic-effects
#8
Franziska Kolrep, Kathleen Rein, Alfonso Lampen, Stefanie Hessel
The lipophilic marine biotoxin okadaic acid (OA) represents a natural contaminant produced by algae accumulating in seafood. Acute intoxications result in diarrhetic shellfish poisoning causing symptoms like nausea, vomiting and abdominal cramps. OA was preincubated with liver enzymes present in S9 fractions from humans, rats and rats pretreated with enzyme inducers in the presence or absence of the cofactor NADPH to investigate hepatic metabolism. Cytotoxicity was examined in HepG2 cells and metabolites of OA were determined by LC-MS/MS...
April 14, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28405802/vasculo-protective-effect-of-bms-309403-is-independent-of-its-specific-inhibition-of-fatty-acid-binding-protein-4
#9
Yuta Okamura, Kosuke Otani, Akihiro Sekiguchi, Taisuke Kogane, Chiharu Kakuda, Yuzaburo Sakamoto, Tomoko Kodama, Muneyoshi Okada, Hideyuki Yamawaki
Fatty acid-binding protein (FABP) 4 is an adipocytokine mainly expressed in adipocyte and macrophage. Blood FABP4 is related not only to metabolic disorders including insulin resistance and atherosclerosis but also increased blood pressure. We tested the hypothesis that FABP4 plays roles in pathogenesis of hypertension development including proliferation, migration, and inflammation of vascular smooth muscle cells (SMCs) as well as contractile reactivity. FABP4 alone had no influence on proliferation, migration, and inflammation of rat mesenteric arterial SMCs, while it significantly enhanced smooth muscle contraction and increases of systolic blood pressure (SBP) induced by noradrenaline (NA)...
April 13, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28404686/propofol-anesthesia-is-reduced-in-phospholipase-c-related-inactive-protein-type-1-knockout-mice
#10
Yoshikazu Nikaido, Tomonori Furukawa, Shuji Shimoyama, Junko Yamada, Keisuke Migita, Kohei Koga, Tetsuya Kushikata, Kazuyoshi Hirota, Takashi Kanematsu, Masato Hirata, Shinya Ueno
The GABA type A receptor (GABAA-R) is a major target of intravenous anesthetics. Phospholipase C-related inactive protein type-1 (PRIP-1) is important in GABAA-R phosphorylation and membrane trafficking. In this study, we investigated the role of PRIP-1 in general anesthetic action. The anesthetic effects of propofol, etomidate, and pentobarbital were evaluated in wild-type and PRIP-1 knockout (PRIP-1 KO) mice by measuring the latency and duration of loss of righting reflex (LORR) and loss of tail-pinch withdrawal response (LTWR)...
April 12, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/28392064/prenatal-exposure-to-perfluoroalkyl-acids-and-prevalence-of-infectious-diseases-up-to-4years-of-age
#11
Houman Goudarzi, Chihiro Miyashita, Emiko Okada, Ikuko Kashino, Chi-Jen Chen, Sachiko Ito, Atsuko Araki, Sumitaka Kobayashi, Hideyuki Matsuura, Reiko Kishi
Perfluoroalkyl acids (PFAAs) are synthetic chemicals with ability to repel oils and water, and have been widely used in many industrial and household applications such as adhesives and water- and stain-repellent surfaces to nonstick coatings. Animal studies have shown that PFAAs have immunotoxic effects. However, few epidemiological studies have investigated the effects of PFAAs on infectious diseases occurrence. We examined the relationship between prenatal exposure to PFAAs and prevalence of infectious diseases up to 4years of life...
April 6, 2017: Environment International
https://www.readbyqxmd.com/read/28387373/identification-of-a-variant-specific-phosphorylation-of-th2a-during-spermiogenesis
#12
Masashi Hada, Koji Masuda, Kosuke Yamaguchi, Katsuhiko Shirahige, Yuki Okada
Tissue-specific histone variant incorporation into chromatin plays dynamic and important roles in tissue development. Testis is one such tissue, and a number of testis-specific histone variants are expressed that have unique roles. While it is expected that such variants acquire post-transcriptional modifications to be functional, identification of variant-specific histone modifications is challenging because of the high similarity of amino acid sequences between canonical and variant versions. Here we identified a novel phosphorylation on TH2A, a germ cell-specific histone H2A variant...
April 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28383915/limaol-a-polyketide-from-the-benthic-marine-dinoflagellate-prorocentrum-lima
#13
A Reum Yang, Sangbum Lee, Young Du Yoo, Hyung Seop Kim, Eun Ju Jeong, Jung-Rae Rho
Limaol (1), along with a dinophysistoxin 1 derivative and an okadaic acid (OA) derivative, was isolated from the large-scale cultivation of the benthic marine dinoflagellate Prorocentrum lima. The structure of 1 was determined by a combination of NMR spectroscopy and mass spectrometry and contained tetrahydropyran, 1,3,5,7-tetra(methylene)heptane, and octahydrospiro[pyran-2,2'-pyrano[3,2-b]pyran] moieties. The absolute configuration of 1 was completely elucidated on the basis of ROESY correlations, J-based configuration analysis, and modified Mosher's ester analysis...
April 6, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28381553/functional-and-biochemical-characterization-of-a-t-cell-associated-anti-apoptotic-protein-gimap6
#14
Ching-Huang Ho, Shih-Feng Tsai
GTPases of immunity-associated proteins (GIMAPs) are expressed in lymphocytes and regulate survival/death signaling and cell development within the immune system. We found that human GIMAP6 is expressed primarily in T cell lines. By sorting human peripheral blood mononuclear cells and performing quantitative RT-PCR, GIMAP6 was found to be expressed in CD3+ cells. In Jurkat cells that had been knocked down for GIMAP6, treatment with hydrogen peroxide, FasL or okadaic acid significantly increased cell death/apoptosis...
April 5, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28381458/protein-phosphatase-2c-pp2c-is-responsible-for-vp-induced-dephosphorylation-of-aqp2-serine-261
#15
Pui W Cheung, Lars Ueberdiek, Jack Day, Richard Bouley, Dennis Brown
AQP2 trafficking is regulated by phosphorylation and dephosphorylation of serine residues in the AQP2 c-terminus. Vasopressin (VP) binding to its receptor (V2R) leads to a cascade of events that result in the phosphorylation of serine 256 (S256), S264 and S269, but dephosphorylation of S261. To identify which phosphatase is responsible for VP-induced S261 dephosphorylation, we pretreated cells with different phosphatase inhibitors before VP stimulation. Only sanguinarine, a specific protein phosphatase 2C (PP2C) inhibitor, abolished VP-induced S261 dephosphorylation, but not inhibitors of PP1, PP2A (okadaic acid) or PP2B (cyclosporine)...
April 5, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28379171/restoration-of-tear-secretion-in-a-murine-dry-eye-model-by-oral-administration-of-palmitoleic-acid
#16
Yuki Kimura, Daisuke Mori, Toshihiro Imada, Yusuke Izuta, Michiko Shibuya, Hisayo Sakaguchi, Erina Oonishi, Naoko Okada, Kenji Matsumoto, Kazuo Tsubota
Sea buckthorn (Hippophae rhamnoides)-derived products have traditionally been used as food and medicinal ingredients in Eastern countries. The purpose of this study was to investigate the effect of oral intake of sea buckthorn oil products on tear secretion using a murine dry eye model. Orally administered sea buckthorn pulp oil (not seed oil) restored aqueous tear secretion to its normal value under a dry eye condition. Palmitoleate (C16:1), a fatty acid present in sea buckthorn pulp oil, preserved tear secretion and suppressed inflammatory cytokines in the lacrimal gland to the same extent as that by pulp oil...
April 5, 2017: Nutrients
https://www.readbyqxmd.com/read/28366788/identification-and-characterization-of-guanosine-5-monophosphate-reductase-of-trypanosoma-congolense-as-a-drug-target
#17
Albertus Eka Yudistira Sarwono, Keisuke Suganuma, Shinya Mitsuhashi, Tadashi Okada, Simon Peter Musinguzi, Kengo Shigetomi, Noboru Inoue, Makoto Ubukata
Trypanosoma congolense is one of the most prevalent pathogens which causes trypanosomosis in African animals, resulting in a significant economic loss. In its life cycle, T. congolense is incapable of synthesizing purine nucleotides via a de novo pathway, and thus relies on a salvage pathway to survive. In this study, we identified a gene from T. congolense, TcIL3000_5_1940, as a guanosine 5'-monophosphate reductase (GMPR), an enzyme that modulates the concentration of intracellular guanosine in the pathogen...
March 30, 2017: Parasitology International
https://www.readbyqxmd.com/read/28366404/sulfated-diesters-of-okadaic-acid-and-dtx-1-self-protective-precursors-of-diarrhetic-shellfish-poisoning-dsp-toxins
#18
Tingmo Hu, Patricia LeBlanc, Ian W Burton, John A Walter, Pearse McCarron, Jeremy E Melanson, Wendy K Strangman, Jeffrey L C Wright
Many toxic secondary metabolites used for defense are also toxic to the producing organism. One important way to circumvent toxicity is to store the toxin as an inactive precursor. Several sulfated diesters of the diarrhetic shellfish poisoning (DSP) toxin okadaic acid have been reported from cultures of various dinoflagellate species belonging to the genus Prorocentrum. It has been proposed that these sulfated diesters are a means of toxin storage within the dinoflagellate cell, and that a putative enzyme mediated two-step hydrolysis of sulfated diesters such as DTX-4 and DTX-5 initially leads to the formation of diol esters and ultimately to the release of free okadaic acid...
March 2017: Harmful Algae
https://www.readbyqxmd.com/read/28363841/aca-an-inhibitor-phospholipases-a2-and-transient-receptor-potential-melastatin-2-channels-attenuates-okadaic-acid-induced-neurodegeneration-in-rats
#19
Murat Cakir, Halil Duzova, Suat Tekin, Elif Taslıdere, Gul Busra Kaya, Yılmaz Cigremis, Tuba Ozgocer, Saim Yologlu
AIM: In recent studies, it has been shown that the Transient Receptor Potential Melastatin-2 Channels (TRPM2) and Phospholipases A2 (PLA2) inhibitors may have a protective effect on neurons. This study was aimed to investigate the protective effect of TRPM2 and PLA2 inhibitor N-(p-amylcinnamoyl) Anthranilic Acid (ACA) in a neurodegenerative model induced by Okadaic Acid (OKA). MAIN METHODS: OKA (200ng/10μl) was administered bilateral intracerebroventricularly as a single injection...
May 1, 2017: Life Sciences
https://www.readbyqxmd.com/read/28359686/slm-a-novel-carbazole-based-fluorophore-attenuates-okadaic-acid-induced-tau-hyperphosphorylation-via-down-regulating-gsk-3%C3%AE-activity-in-sh-sy5y-cells
#20
Xiaoli Wu, Jayasankar Kosaraju, Kin Yip Tam
Phosphorylated tau dissociates from microtubules and aggregates to form neurofibrillary tangles resulting in neuronal toxicity and cognitive deficits. Attenuating tau hyperphosphorylation is considered as an effective therapeutic approach for Alzheimer's disease (AD). From our previous study, SLM, a carbazole-based fluorophore prevents Aβ aggregation, reduced glycogen synthase kinase-3β (GSK-3β) activity and tau hyperphosphorylation in triple transgenic mouse model of AD. However, the mechanism by which SLM attenuates tau hyperphosphorylation warrants further investigation...
March 27, 2017: European Journal of Pharmaceutical Sciences
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