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okadaic acid

Jun Hirata, Tomomitsu Hirota, Takeshi Ozeki, Masahiro Kanai, Takeaki Sudo, Toshihiro Tanaka, Nobuyuki Hizawa, Hidemi Nakagawa, Shinichi Sato, Taisei Mushiroda, Hidehisa Saeki, Mayumi Tamari, Yukinori Okada
Psoriasis vulgaris (PsV) is an autoimmune disease of skin and joints with heterogeneity in epidemiological and genetic landscapes of global populations. We conducted an initial genome-wide association study (GWAS) and a replication study of PsV in the Japanese population (606 PsV cases and 2,052 controls). We identified significant associations of the SNPs with PsV risk at TNIP1 and the MHC region (P = 3.7×10(-10) and 6.6×10(-15), respectively). By updating the HLA imputation reference panel of Japanese (n = 908) to expand HLA gene coverage, we fine-mapped the HLA variants associated with PsV risk...
October 12, 2017: Journal of Investigative Dermatology
Fumiaki Beppu, Keiko Yasuda, Ayako Okada, Yoshitsugu Hirosaki, Masako Okazaki, Naohiro Gotoh
Highly unsaturated fatty acid (HUFA) binding at the sn-2 position of phospholipids (PL) becomes a resource for prostaglandin, leukotriene, resolvin, and protectin synthesis. Both triacylglycerol (TAG) and PL synthesis pathways in vivo are via phosphatidic acid; therefore, the distribution of fatty acid species at the sn-2 position must theoretically be the same for TAG and PL if rearrangement does not occur. However, it is known that little HUFA is located at the sn-2 position of TAG in marine mammals. Therefore, distribution of fatty acid species at the sn-2 position of TAG and PL was compared between marine fishes and mammals in this study...
October 11, 2017: Journal of Oleo Science
Maria João Botelho, Carlos Vale, Sandra Joaquim, Sara T Costa, Florbela Soares, Claúdia Roque, Domitília Matias
The influence of nutritional regime and water temperature on depuration rates of OA-group toxins in the wedge shell Donax trunculus was examined by exposing naturally contaminated specimens to three nutritional regimes (microalgae, commercial paste of microalgae, and starvation) for 14 days at 16 °C and 20 °C. Total OA was quantified in the whole soft tissues of the individuals collected in days 2, 4, 6, 8, 10, 12 and 14. Mortality, dry weight, condition index, gross biochemical composition and gametogenic stages were surveyed...
September 21, 2017: Chemosphere
Ying Wang, Yanli Xia, Dong Kuang, Yaqi Duan, Guoping Wang
AIMS: Previous studies have shown that stem cell factor (SCF) induces the migration of cardiac stem cells (CSCs) and helps to repair myocardial infarctions. Earlier studies on the migration mechanism only focused on the activation of kinases; here, we aimed to explore the functional role of protein phosphatase 2A (PP2A) in SCF-induced CSC migration. MAIN METHODS: CSCs were treated with SCF, PP2A enzymatic activity was measured, the phosphorylation levels of PP2A, p38 MAPK and cofilin were evaluated using western blot...
October 3, 2017: Life Sciences
Sahoko Takagi, Mariko Naito, Sayo Kawai, Rieko Okada, Chisato Nagata, Satoyo Hosono, Yuichiro Nishida, Naoyuki Takashima, Sadao Suzuki, Ippei Shimoshikiryo, Haruo Mikami, Hirokazu Uemura, Nagato Kuriyama, Keizo Ohnaka, Michiaki Kubo, Nobuyuki Hamajima, Hideo Tanaka, Kenji Wakai
Although higher circulating levels of oestrogen are related to postmenopausal breast cancer risk, limited information is available regarding effects of diet on endogenous oestrogen. Thus, we examined associations between macronutrient intakes and serum oestrogen with consideration of polymorphisms in oestrogen-metabolising genes. In this cross-sectional study, 784 naturally menopaused Japanese women aged 47-69 years were selected from participants of the Japan Multi-Institutional Collaborative Cohort Study...
September 2017: British Journal of Nutrition
Keita Nishiyama, Yuji Yamamoto, Makoto Sugiyama, Takashi Takaki, Tadasu Urashima, Satoru Fukiya, Atsushi Yokota, Nobuhiko Okada, Takao Mukai
Bifidobacterium is a natural inhabitant of the human gastrointestinal (GI) tract. We studied the role of the extracellular sialidase (SiaBb2, 835 amino acids [aa]) from Bifidobacterium bifidum ATCC 15696 in mucosal surface adhesion and carbohydrate catabolism. Human milk oligosaccharides (HMOs) or porcine mucin oligosaccharides as the sole carbon source enhanced B. bifidum growth. This was impaired in a B. bifidum ATCC 15696 strain harboring a mutation in the siabb2 gene. Mutant cells in early to late exponential growth phase also showed decreased adhesion to human epithelial cells and porcine mucin relative to the wild-type strain...
October 3, 2017: MBio
Lei Pan, Junhui Chen, Huihui Shen, Xiuping He, Guangjiu Li, Xincheng Song, Deshan Zhou, Chengjun Sun
Extracellular toxins released by marine toxigenic algae into the marine environment have attracted increasing attention in recent years. In this study, profiling, characterization and quantification of extracellular toxin compounds associated with diarrhetic shellfish poison (DSP) in the culture medium of toxin-producing dinoflagellates were performed using high-performance liquid chromatography-high-resolution mass spectrometry/tandem mass spectrometry for the first time. Results showed that solid-phase extraction can effectively enrich and clean the DSP compounds in the culture medium of Prorocentrum lima (P...
September 30, 2017: Toxins
Mitsuru Maruno, Masamichi Shinoda, Kuniya Honda, Reio Ito, Kentaro Urata, Masahiro Watanabe, Shinji Okada, Jun Lee, Nobuhito Gionhaku, Koichi Iwata
AIMS: To develop a tongue pain model with no mucosal pathologic changes and to examine whether phosphorylation of p38 in trigeminal ganglion (TG) neurons innervating the tongue is associated with tongue heat hypersensitivity in mice. METHODS: Tongue heat sensitivity in mice was assessed following application of the irritant 2,4,6-trinitrobenzene sulfonic acid (TNBS) to the tongue. After TNBS application, the expressions of p38, phosphorylated p38 (pp38), and transient receptor potential vanilloid 1 (TRPV1) were examined in TG neurons innervating the tongue...
October 3, 2017: Journal of Oral & Facial Pain and Headache
Valentina Giussani, Valentina Asnaghi, Andrea Pedroncini, Mariachiara Chiantore
Concern regarding Benthic Harmful Algal Blooms (BHABs) is increasing since some harmful benthic species have been identified in new areas. In the Mediterranean basin, the most common harmful benthic microalgae are Ostreopsis cf. ovata and Prorocentrum lima, which produce palytoxin-like compounds and okadaic acid respectively, and the need to implement monitoring activities has increased. However, a general agreement on appropriate strategies (e.g. sampling season, definition of alarm thresholds, etc.) is still lagging behind, especially for P...
September 2017: Harmful Algae
Raku Irie, Kentaro Takada, Yuji Ise, Susumu Ohtsuka, Shigeru Okada, Kirk R Gustafson, Shigeki Matsunaga
The planar structure of poecillastrin C (1) was revised through selective reduction of the ester carbon. The absolute configuration of the β-hydroxyaspartic acid (OHAsp) residue was determined to be d-threo by Marfey's analysis. The acid hydrolysate of the reduction product of 1 liberated (2R,3R)-2-amino-3,4-dihydroxybutanoic acid, demonstrating that the β-carboxyl group in poecillastrin C was esterified. The structures of poecillastrins B-D and 73-deoxychondropsin A were also revised.
October 6, 2017: Organic Letters
Hirota Fujiki, Eisaburo Sueoka, Anchalee Rawangkan, Masami Suganuma
PURPOSE: Our previous experiments show that the main constituent of green-tea catechins, (-)-epigallocatechin gallate (EGCG), completely prevents tumor promotion on mouse skin initiated with 7,12-dimethylbenz(a)anthracene followed by okadaic acid and that EGCG and green tea extract prevent cancer development in a wide range of target organs in rodents. Therefore, we focused our attention on human cancer stem cells (CSCs) as targets of cancer prevention and treatment with EGCG. METHODS: The numerous reports concerning anticancer activity of EGCG against human CSCs enriched from cancer cell lines were gathered from a search of PubMed, and we hope our review of the literatures will provide a broad selection for the effects of EGCG on various human CSCs...
September 23, 2017: Journal of Cancer Research and Clinical Oncology
Marcia Bodero, Ron L A P Hoogenboom, Toine F H Bovee, Liza Portier, Laura de Haan, Ad Peijnenburg, Peter J M Hendriksen
A study with DNA microarrays was performed to investigate the effects of two diarrhetic and one azaspiracid shellfish poison, okadaic acid (OA), dinophysistoxin-1 (DTX-1) and azaspiracid-1 (AZA-1) respectively, on the whole-genome mRNA expression of undifferentiated intestinal Caco-2 cells. Previously, the most responding genes were used to develop a dedicated array tube test to screen shellfish samples on the presence of these toxins. In the present study the whole genome mRNA expression was analyzed in order to reveal modes of action and obtain hints on potential biomarkers suitable to be used in alternative bioassays...
September 20, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Joana Machado Oliveira, Cristóvão B da Cruz E Silva, Thorsten Müller, Tânia Soares Martins, Marta Cova, Odete A B da Cruz E Silva, Ana Gabriela Henriques
Neuroproteomics is an evolving field of postgenomic medicine, highlighting the convergence of psychiatry/neurology and proteomics, yet compared with neurogenetics, it has received little attention. This study in rat primary neuronal cultures provides an example of a neuroproteomic approach relevant to the study of psychiatric disease pathophysiology, focusing on Alzheimer's disease. In this context, okadaic acid (OA) is routinely used in experimental designs to investigate phosphorylation-mediated events. It is a potent protein phosphatase (PP) inhibitor, particularly of PP1 and PP2A...
September 2017: Omics: a Journal of Integrative Biology
Gábor Gullner, Csilla Juhász, Adél Németh, Balázs Barna
The interactions of powdery mildew (Golovinomyces orontii) and Tobacco mosaic virus (TMV) with tobacco lines having down or upregulated antioxidants were investigated. Xanthi-nc, its salicylic acid-deficient NahG mutant, a paraquat-sensitive Samsun (PS) and its paraquat tolerant (PT) mutant were used. Cell membrane damage caused by H2O2 was significantly higher in NahG than Xanthi, whereas it was lower in PT than in PS. Leakage of ions from PT was reduced by the powdery mildew infection. On the other hand TMV inoculation led to a 6-fold and 2-fold elevation of ion leakage from hypersensitive resistant NahG and Xanthi leaves, respectively, whereas ion leakage increased slightly from susceptible PS leaves...
September 8, 2017: Plant Physiology and Biochemistry: PPB
Junhui Chen, Tongzhu Han, Xiaotong Li, Xiuping He, Yanlong Wang, Farong Chen, Xincheng Song, Deshan Zhou, Xiaoru Wang
For the first time, the composition, concentration and distribution characteristics of typical lipophilic marine algal toxins (LMATs) are investigated in surface seawater, suspended particulate matter (SPM) and sediments from the Yellow Sea and the Bohai Sea, China. Pectenotoxin-2 (PTX2) and okadaic acid (OA) were detected in offshore surface seawater samples (n=67) of the Yellow and Bohai Seas, and PTX2 was found in higher concentrations than OA. The concentrations of PTX2 were between 0.49 and 14.14ng/L. OA, dinophysistoxin-1(DTX1), PTX2 and gymnodimine (GYM) were detected in the nearshore surface seawater samples (n=20) of the Haizhou Bay of the Yellow Sea...
September 5, 2017: Science of the Total Environment
Keisuke Nakagawa, Kento Nakagawa, Tsutomu Omatsu, Yukie Katayama, Mami Oba, Hiromichi Mitake, Kazuma Okada, Satoko Yamaoka, Yasuhiro Takashima, Tatsunori Masatani, Kota Okadera, Naoto Ito, Tetsuya Mizutani, Makoto Sugiyama
The current live rabies vaccine SAG2 is attenuated by only one mutation (Arg-to-Glu) at position 333 in the glycoprotein (G333). This fact generates a potential risk of the emergence of a pathogenic revertant by a back mutation at this position during viral propagation in the body. To circumvent this risk, it is desirable to generate a live vaccine strain highly and stably attenuated by multiple mutations. However, the information on attenuating mutations other than that at G333 is very limited. We previously reported that amino acids at positions 273 and 394 in the nucleoprotein (N273/394) (Leu and His, respectively) of fixed rabies virus Ni-CE are responsible for the attenuated phenotype by enhancing interferon (IFN)/chemokine gene expressions in infected neural cells...
September 4, 2017: Vaccine
Shotaro Hoshino, Masahiro Okada, Takayoshi Awakawa, Shumpei Asamizu, Hiroyasu Onaka, Ikuro Abe
Two novel macrolactams, dracolactams A and B, were identified from a combined-culture of Micromonospora species and a mycolic-acid containing bacterium (MACB). Their structures and stereochemistries were completely assigned, based on spectroscopic analyses and chemical derivatization. Both dracolactams were probably generated from a common macrolactam precursor produced by the Micromonospora species. In this combined-culture system, MACB is likely to activate cryptic oxidase genes in the Micromonospora species and induce the downstream polyene macrolactam cyclization...
September 7, 2017: Organic Letters
Yoshiyasu Kono, Yoshiro Kawahara, Hiroyuki Okada
No abstract text is available yet for this article.
August 30, 2017: Clinical Gastroenterology and Hepatology
Yuri Yoshida, Koji Miyamoto, Hisakazu Yamane, Yoko Nishizawa, Eiichi Minami, Hideaki Nojiri, Kazunori Okada
Phytocassanes and momilactones are known as major diterpenoid phytoalexins (DPs), characterized by abundant production and antimicrobial activity and their biosynthetic genes are clustered in rice genomes. The basic leucine zipper transcription factor OsTGAP1 is known to act as a regulator of the coordinated production of DPs in cultured rice cells, but the in planta functions of OsTGAP1 remain largely unknown. Here, we present evidence on the biological function of OsTGAP1 in planta. In wild-type plants, OsTGAP1 is abundantly expressed in roots compared to that in shoots...
August 30, 2017: Physiologia Plantarum
Akihiko Ono, Hiroaki Azukizawa, Sayami Ito, Yuki Nakamura, Hideo Asada, Ying-Shu Quan, Fumio Kamiyama, Ichiro Katayama, Sachiko Hirobe, Naoki Okada
Microneedle (MN) patches have great potential as transcutaneous vaccine delivery devices because MNs can effectively deliver vaccine antigen into the skin through the micropores formed in the stratum corneum by low-invasive and painless skin puncturing. This study aims to develop novel double-decker MN patches which have not only high safety and efficacy but also broad applicability to various vaccine antigens. We developed two types of MN patches (PGA-MN and Nylon-MN) that are made from polyglycolic acid and Nylon-6...
August 30, 2017: International Journal of Pharmaceutics
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