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okadaic acid

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https://www.readbyqxmd.com/read/29222112/rheb-localized-on-the-golgi-membrane-activates-lysosome-localized-mtorc1-at-the-golgi-lysosome-contact-site
#1
Feike Hao, Kazuhiko Kondo, Takashi Itoh, Sumiko Ikari, Shigeyuki Nada, Masato Okada, Takeshi Noda
In response to amino acid supply, mTORC1, a master regulator of cell growth, is recruited to the lysosome and activated by a small GTPase, Rheb. However, the intracellular localization of Rheb is controversial. In this study, we showed that a significant portion of Rheb was localized on the Golgi but not on the lysosome. GFP-Rheb could activate mTORC1, even when forced to exclusively localize to the Golgi. Likewise, artificial recruitment of mTORC1 to the Golgi allowed its activation. Accordingly, the Golgi was in contact with the lysosome at the newly described Golgi lysosome contact site (GLCS)...
December 8, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/29207020/quercetin-inhibits-okadaic-acid-induced-tau-protein-hyperphosphorylation-through-the-ca2-%C3%A2-calpain%C3%A2-p25%C3%A2-cdk5-pathway-in-ht22-cells
#2
Xiu-Yin Shen, Tao Luo, Sheng Li, Ou-Yang Ting, Feng He, Jie Xu, Hua-Qiao Wang
Alzheimer's disease (AD) is a common neurodegenerative disorder characterized by aberrant tau protein hyperphosphorylation, which eventually leads to the formation of neurofibrillary tangles. Hyperphosphorylated tau protein is considered as a vital factor in the development of AD and is highly associated with cognitive impairment. Therefore, it is recognized to be a potential therapeutic target. Quercetin (QUE) is a naturally occurring flavonoid compound. In the present study, the inhibitory effect of QUE on okadaic acid (OA)-induced tau protein hyperphosphorylation in HT22 cells was explored...
November 22, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29203539/novel-and-shared-neoantigen-derived-from-histone-3-variant-h3-3k27m-mutation-for-glioma-t-cell-therapy
#3
Zinal S Chheda, Gary Kohanbash, Kaori Okada, Naznin Jahan, John Sidney, Matteo Pecoraro, Xinbo Yang, Diego A Carrera, Kira M Downey, Shruti Shrivastav, Shuming Liu, Yi Lin, Chetana Lagisetti, Pavlina Chuntova, Payal B Watchmaker, Sabine Mueller, Ian F Pollack, Raja Rajalingam, Angel M Carcaboso, Matthias Mann, Alessandro Sette, K Christopher Garcia, Yafei Hou, Hideho Okada
The median overall survival for children with diffuse intrinsic pontine glioma (DIPG) is less than one year. The majority of diffuse midline gliomas, including more than 70% of DIPGs, harbor an amino acid substitution from lysine (K) to methionine (M) at position 27 of histone 3 variant 3 (H3.3). From a CD8+ T cell clone established by stimulation of HLA-A2+ CD8+ T cells with synthetic peptide encompassing the H3.3K27M mutation, complementary DNA for T cell receptor (TCR) α- and β-chains were cloned into a retroviral vector...
December 4, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/29186909/ketamine-a-clinically-used-anesthetic-inhibits-vascular-smooth-muscle-cell-proliferation-via-pp2a-activated-pi3k-akt-erk-inhibition
#4
Yi Chang, Jiun-Yi Li, Thanasekaran Jayakumar, Shou-Huang Hung, Wei-Cheng Lee, Manjunath Manubolu, Joen-Rong Sheu, Ming-Jen Hsu
Abnormal proliferation of vascular smooth muscle cells (VSMCs) gives rise to major pathological processes involved in the development of cardiovascular diseases. The use of anti-proliferative agents for VSMCs offers potential for the treatment of vascular disorders. Intravenous anesthetics are firmly established to have direct effects on VSMCs, resulting in modulation of blood pressure. Ketamine has been used for many years in the intensive care unit (ICU) for sedation, and has recently been considered for adjunctive therapy...
November 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29175632/intravenous-administration-of-the-adeno-associated-virus-php-b-capsid-fails-to-upregulate-transduction-efficiency-in-the-marmoset-brain
#5
Yasunori Matsuzaki, Ayumu Konno, Ryuta Mochizuki, Yoichiro Shinohara, Keisuke Nitta, Yukihiro Okada, Hirokazu Hirai
Intravenous administration of adeno-associated virus (AAV)-PHP.B, a capsid variant of AAV9 containing seven amino acid insertions, results in a greater permeability of the blood brain barrier (BBB) than standard AAV9 in mice, leading to highly efficient and global transduction of the central nervous system (CNS). The present study aimed to examine whether the enhanced BBB penetrance of AAV-PHP.B observed in mice also occurs in non-human primates. Thus, a young adult (age, 1.6 years) and an old adult (age, 7...
November 23, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/29174400/self-assembled-monolayer-based-immunoassays-for-okadaic-acid-detection-in-seawater-as-monitoring-tools
#6
Sandra Leonardo, Anna Toldrà, Maria Rambla-Alegre, Margarita Fernández-Tejedor, Karl B Andree, Laura Ferreres, Katrina Campbell, Christopher T Elliott, Ciara K O'Sullivan, Yolanda Pazos, Jorge Diogène, Mònica Campàs
Rapid and cost-effective methods to monitor the presence of diarrhetic shellfish poisoning (DSP) toxins in seawater samples in an easy and reliable manner are required to protect human health and avoid economic losses to shellfish industry. Immunoassays for the detection of okadaic acid (OA) and dinophysistoxin-1 and dinophysistoxin-2 are developed by immobilising OA on self-assembled monothiols or dithiols in an ordered and oriented way, providing an effective limit of detection of ∼1 ng OA equiv./mL seawater...
November 14, 2017: Marine Environmental Research
https://www.readbyqxmd.com/read/29169570/morphology-and-phylogeny-of-prorocentrum-caipirignum-sp-nov-dinophyceae-a-new-tropical-toxic-benthic-dinoflagellate
#7
Silvia M Nascimento, M Cristina Q Mendes, Mariângela Menezes, Francisco Rodríguez, Catharina Alves-de-Souza, Suema Branco, Pilar Riobó, José Franco, José Marcos C Nunes, Mariusz Huk, Steven Morris, Santiago Fraga
A new species of toxic benthic dinoflagellate is described based on laboratory cultures isolated from two locations from Brazil, Rio de Janeiro and Bahia. The morphology was studied with SEM and LM. Cells are elliptical in right thecal view and flat. They are 37-44μm long and 29-36μm wide. The right thecal plate has a V shaped indentation where six platelets can be identified. The thecal surface of both thecal plates is smooth and has round or kidney shaped and uniformly distributed pores except in the central area of the cell, and a line of marginal pores...
December 2017: Harmful Algae
https://www.readbyqxmd.com/read/29158492/structural-basis-for-the-assembly-of-the-ragulator-rag-gtpase-complex
#8
Ryo Yonehara, Shigeyuki Nada, Tomokazu Nakai, Masahiro Nakai, Ayaka Kitamura, Akira Ogawa, Hirokazu Nakatsumi, Keiichi I Nakayama, Songling Li, Daron M Standley, Eiki Yamashita, Atsushi Nakagawa, Masato Okada
The mechanistic target of rapamycin complex 1 (mTORC1) plays a central role in regulating cell growth and metabolism by responding to cellular nutrient conditions. The activity of mTORC1 is controlled by Rag GTPases, which are anchored to lysosomes via Ragulator, a pentameric protein complex consisting of membrane-anchored p18/LAMTOR1 and two roadblock heterodimers. Here we report the crystal structure of Ragulator in complex with the roadblock domains of RagA-C, which helps to elucidate the molecular basis for the regulation of Rag GTPases...
November 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/29151538/iron-deficiency-anemia-due-to-the-long-term-use-of-a-proton-pump-inhibitor
#9
Ryosuke Imai, Takakazu Higuchi, Masaya Morimoto, Ryosuke Koyamada, Sadamu Okada
A 52-year-old man who had been taking omeprazole, a proton pump inhibitor (PPI), for 25 years developed iron deficiency anemia. An evaluation of the entire gastrointestinal tract did not reveal any possible causes of gastrointestinal blood loss. The cause of the iron deficiency was considered to be a reduction in gastrointestinal iron absorption in association with the reduced secretion of gastric acid due to PPI use. This case demonstrates that long-term PPI use for as long as 25 years may cause iron deficiency anemia and should be considered in the differential diagnosis of iron deficiency anemia in long-term PPI users...
November 20, 2017: Internal Medicine
https://www.readbyqxmd.com/read/29149435/equine-herpesvirus-type-1-orf51-encoding-ul11-as-an-essential-gene-for-replication-in-cultured-cells
#10
Yassien Badr, Ayaka Okada, Rania Abo-Sakaya, Emad Beshir, Kenji Ohya, Hideto Fukushi
Equine herpesvirus type 1 (EHV-1) UL11 is a 74-amino-acid tegument protein encoded by ORF51 of the EHV-1 genome. EHV-1 UL11 was previously reported by other researchers using the RacL22 and RacH strains to be nonessential for viral replication in cultured cells. Here, we constructed UL11 mutant viruses including a UL11 null mutant and three C-terminal truncated mutants, for further characterization of EHV-1 UL11 using bacterial artificial chromosome (BAC) technology based on the neuropathogenic strain Ab4p...
November 17, 2017: Archives of Virology
https://www.readbyqxmd.com/read/29138881/a-harmonized-immunoassay-with-liquid-chromatography-mass-spectrometry-analysis-in-egg-allergen-determination
#11
Masaomi Nimata, Hideki Okada, Kei Kurihara, Tsukasa Sugimoto, Tsutomu Honjoh, Kazuhiko Kuroda, Takeo Yano, Hirofumi Tachibana, Masahiro Shoji
Food allergy is a serious health issue worldwide. Implementing allergen labeling regulations is extremely challenging for regulators, food manufacturers, and analytical kit manufacturers. Here we have developed an "amino acid sequence immunoassay" approach to ELISA. The new ELISA comprises of a monoclonal antibody generated via an analyte specific peptide antigen and sodium lauryl sulfate/sulfite solution. This combination enables the antibody to access the epitope site in unfolded analyte protein. The newly developed ELISA recovered 87...
November 14, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29123986/expression-of-a-urokinase-type-plasminogen-activator-during-tumor-growth-leads-to-angiogenesis-via-galanin-activation-in-tumor-bearing-mice
#12
Hiroyuki Yamamoto, Rina Okada, Rika Tanaka, Keiko Unno, Kazuaki Iguchi
Small-cell lung carcinoma releases progalanin. The released progalanin is activated via a nonclassical processing pathway, being processed into an active form of galanin (1-20) by plasmin in extracellular components. Plasmin is produced from plasminogen activators. To clarify the regulation of progalanin via plasminogen activation by urokinase and tissue-plasminogen activator (t-PA), we investigated the regulation mechanism for urokinase and t-PA expression and their effect on galanin activation. Additionally, we studied the effect of activated galanin on angiogenesis...
November 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/29103093/enhancement-of-gustatory-neural-responses-by-parasympathetic-nerve-in-the-frog
#13
Toshihide Sato, Yukio Okada
The autonomic nervous system affects the gustatory responses in animals. Frog glossopharyngeal nerve (GPN) contains the parasympathetic nerve. We checked the effects of electrical stimulation (ES) of the parasympathetic nerves on the gustatory neural responses. The gustatory neural impulses of the GPNs were recorded using bipolar AgCl wires under normal blood circulation and integrated with a time constant of 1 s. Electrical stimuli were applied to the proximal side of the GPN with a pair of AgCl wires. The parasympathetic nerves of the GPN were strongly stimulated for 10 s with 6 V at 30 Hz before taste stimulation...
November 4, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/29095659/indirect-activation-of-pregnane-x-receptor-in-the-induction-of-hepatic-cyp3a11-by-high-dose-rifampicin-in-mice
#14
Yuki Yamasaki, Kaoru Kobayashi, Asumi Inaba, Daisuke Uehara, Hiroki Tojima, Satoru Kakizaki, Kan Chiba
Rifampicin (RIF), a typical ligand of human pregnane X receptor (PXR), powerfully induces the expression of cytochrome P450 3A4 (CYP3A4) in humans. Although it is thought that RIF is not a ligand of rodent PXR, treatment with high-dose RIF (e.g., more than 20 mg/kg) increases the expression of CYP3A in the mouse liver. In this study, we investigated whether the induction of CYP3A by high-dose RIF in the mouse liver is mediated via indirect activation of mouse PXR (mPXR). The results showed that high-dose RIF increased the expression of CYP3A11 and other PXR-target genes in the liver of wild-type mice but not PXR-knockout mice...
November 2, 2017: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/29082049/a-calcium-enterolith-in-a-patient-with-crohn-s-disease-and-its-in-vitro-dissolubility-in-citric-acid
#15
Masaya Iwamuro, Haruo Urata, Sakiko Hiraoka, Masayasu Ohmori, Yoshitaka Kondo, Yoshiro Kawahara, Hiroyuki Okada
The microstructure and dissolubility of a calcified enterolith and enterolith pieces removed from a 26-year-old Japanese woman with Crohn's disease were analyzed using scanning electron microscopy and energy dispersive X-ray spectroscopy. The enterolith showed a multilayered structure with fatty acid calcium and magnesium phosphate. The amount of calcium, magnesium, and phosphate decreased after they were immersed in a citric acid solution, suggesting a potential contribution of acidic aqueous solution to elute inorganic substances contained in calcified enteroliths...
2017: Case Reports in Gastrointestinal Medicine
https://www.readbyqxmd.com/read/29080939/a-common-variant-of-maf-c-maf-transcriptional-factor-gene-in-the-kidney-is-associated-with-gout-susceptibility
#16
Toshihide Higashino, Hirotaka Matsuo, Yukinori Okada, Hiroshi Nakashima, Seiko Shimizu, Masayuki Sakiyama, Shin Tadokoro, Akiyoshi Nakayama, Makoto Kawaguchi, Mako Komatsu, Asahi Hishida, Masahiro Nakatochi, Hiroshi Ooyama, Junko Imaki, Nariyoshi Shinomiya
Gout is a multifactorial disease characterized by acute inflammatory arthritis, and it is caused as a consequence of hyperuricemia. A recent meta-analysis of genome-wide association studies has newly identified the relationship between serum uric acid (SUA) levels and rs889472, a single nucleotide polymorphism of musculoaponeurotic fibrosarcoma oncogene (MAF/c-MAF). However, it remained unclear whether rs889472 is associated with gout susceptibility. In the present study, we investigate the association between c-MAF rs889472 and gout in Japanese male population...
October 28, 2017: Human Cell
https://www.readbyqxmd.com/read/29066793/phenazine-carboxylic-acid-and-its-derivative-induce-osteoblast-differentiation-in-preosteoblastic-mc3t3-e1-cells-but-adipocyte-differentiation-in-pluripotent-mesenchymal-c3h10t1-2-cells
#17
Shuichi Sakamoto, Takumi Watanabe, Yasuko Kohda, Masatomi Iijima, Ryuichi Sawa, Maiko Okada, Hayamitsu Adachi, Yoshio Nishimura, Manabu Kawada
Osteoblast and adipocyte are differentiated from mesenchymal stem cells and dysregulation of the differentiation might result in disease, such as osteoporosis and diabetes. To find small compounds that induce osteoblast differentiation, we screened an in-house natural compounds library with mouse preosteoblastic MC3T3-E1 cells using alkaline phosphatase (ALP) expression as an early osteoblast marker. We found that phenazine-1-carboxylic acid (PCA), one of the major phenazine derivatives produced by Pseudomonas, induced osteoblast differentiation in the cells at micromolar concentrations...
October 25, 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/29053076/protective-effect-of-biodegradable-nerve-conduit-against-peripheral-nerve-adhesion-after-neurolysis
#18
Kosuke Shintani, Takuya Uemura, Kiyohito Takamatsu, Takuya Yokoi, Ema Onode, Mitsuhiro Okada, Hiroaki Nakamura
OBJECTIVE Peripheral nerve adhesion caused by extraneural and intraneural scar formation after neurolysis leads to nerve dysfunction. The authors previously developed a novel very flexible biodegradable nerve conduit composed of poly(L-lactide) and poly(ε-caprolactone) for use in peripheral nerve regeneration. In the present study, they investigated the effect of protective nerve wrapping on preventing adhesion in a rat sciatic nerve adhesion model. METHODS Rat sciatic nerves were randomly assigned to one of the following four groups: a no-adhesion group, which involved neurolysis alone without an adhesion procedure; an adhesion group, in which the adhesion procedure was performed after neurolysis, but no treatment was subsequently administered; a nerve wrap group, in which the adhesion procedure was performed after neurolysis and protective nerve wrapping was then performed with the nerve conduit; and a hyaluronic acid (HA) group, in which the adhesion procedure was performed after neurolysis and nerve wrapping was then performed with a 1% sodium HA viscous solution...
October 20, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/29052147/pulsed-light-reduces-the-toxicity-of-the-algal-toxin-okadaic-acid-to-freshwater-crustacean-daphnia-pulex
#19
Iain M T Murray, Neil J Rowan, Sara McNamee, Katrina Campbell, Andrew M Fogarty
This constitutes the first study to report on the reduction in toxicity of the dinoflagellate algal toxin okadaic acid after novel pulsed light (PL) treatments where ecotoxicological assessment was performed using a miniaturised format of the conventional in vivo freshwater crustacean Daphnia sp. acute toxicity test. Bivalves accumulate this toxin, which can then enter the human food chain causing deleterious health effects such as diarrhetic shellfish poisoning. This miniaturised toxicological bioassay used substantially less sample volume and chemical reagents...
October 19, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29040229/development-of-a-liquid-chromatography-tandem-mass-spectrometric-method-for-quantification-of-mycophenolic-acid-and-its-glucuronides-in-dried-blood-spot-samples
#20
Hiromasa Iboshi, Hiroaki Yamaguchi, Hiroyuki Suzuki, Masafumi Kikuchi, Masaki Tanaka, Shinya Takasaki, Akiko Takahashi, Masamitsu Maekawa, Miki Shimada, Yasushi Matsuda, Yoshinori Okada, Nariyasu Mano
BACKGROUND: Personalized immunosuppressive therapy, including accurate drug dosing based on the drug blood level, leads to better clinical outcomes, specifically regarding avoidance of drug-induced adverse effects and maintenance of efficacy. Mycophenolic acid (MPA) is used as an immunosuppressant in transplantation of various solid organs. The aim of this study was to develop a method for quantification of MPA and its metabolites, mycophenolic acid 7-O-glucuronide (MPAG) and mycophenolic acid acyl glucuronide, in dried blood spot (DBS) samples, using liquid chromatography/electrospray ionization/tandem mass spectrometry...
December 2017: Therapeutic Drug Monitoring
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