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https://www.readbyqxmd.com/read/27881051/mechanisms-of-immune-suppression-utilized-by-canine-adipose-and-bone-marrow-derived-mesenchymal-stem-cells
#1
Lyndah Chow, Valerie Johnson, Jonathan W Coy, Daniel P Regan, Steven W Dow
Mesenchymal stem cells (MSC) from rodents and humans have been shown to suppress T cells by distinct primary pathways, with NO-dependent pathways dominating in rodents and IDO-dependent pathways dominating in humans. However, the immune suppressive pathways utilized by canine MSC have not been as thoroughly studied, nor have BM-MSC and Ad-MSC been directly compared for their immune modulatory potency or pathway utilization. Therefore, canine BM-MSC and Ad-MSC were generated in vitro and their potency in suppressing T cell proliferation and cytokine production was compared, as well as differential gene expression...
November 23, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27878275/method-for-in%C3%A2-vitro-differentiation-of-bone-marrow-mesenchymal-stem-cells-into-endothelial-progenitor-cells-and-vascular-endothelial-cells
#2
Qihong Wang, Weifeng Zhang, Guifen He, Huifang Sha, Zhe Quan
Vascular development is a regulated process and is dependent on the participation and differentiation of many cell types including the proliferation and migration of vascular endothelial cells and differentiation of endothelial progenitor cells (EPCs) to mesodermal precursor cells. Thus, reconstitution of this process in vitro necessitates providing ambient conditions for generating and culturing EPCs in vitro and differentiating them to vascular endothelial cells. In the present study, we developed methods to differentiate bone marrow mesenchymal stem cells (MSC) into EPCs and to vascular endothelial cells...
November 18, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27876576/the-bone-regenerative-capacity-of-canine-mesenchymal-stem-cells-is-regulated-by-site-specific-multilineage-differentiation
#3
Juan Bugueño, Weihua Li, Pinky Salat, Ling Qin, Sunday O Akintoye
OBJECTIVES: Mesenchymal stem cells (MSCs) offer a promising therapy in dentistry because of their multipotent properties. Selecting donor MSCs is crucial because Beagle dogs (canines) commonly used in preclinical studies have shown variable outcomes, and it is unclear whether canine MSCs (cMSCs) are skeletal site specific. This study tested whether jaw and long bone cMSCs have disparate in vitro and in vivo multilineage differentiation capabilities. STUDY DESIGN: Primary cMSCs were isolated from the mandible (M-cMSCs) and femur (F-cMSCs) of four healthy Beagle dogs...
September 28, 2016: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/27861292/long-term-tolerance-toward-haploidentical-vascularized-composite-allograft-transplantation-in-a-canine-model-using-bone-marrow-or-mobilized-stem-cells
#4
Jeff Chang, Scott S Graves, Tiffany Butts-Miwongtum, George E Sale, Rainer Storb, David Woodbridge Mathes
BACKGROUND: The development of safe and reliable protocols for the transplantation of the face and hands may be accomplished with animal modeling of transplantation of vascularized composite allografts (VCA). Previously, we demonstrated that tolerance to a VCA could be achieved after canine recipients were simultaneously given marrow from a dog leukocyte antigen-identical donor. In the present study, we extend those findings across a dog leukocyte antigen mismatched barrier. METHODS: Eight recipient dogs received total body irradiation (4...
December 2016: Transplantation
https://www.readbyqxmd.com/read/27842458/suppression-of-canine-dendritic-cell-activation-maturation-and-inflammatory-cytokine-release-by-mesenchymal-stem-cells-occurs-via-multiple-distinct-biochemical-pathways
#5
William Henry Wheat, Lyndah Chow, Jade N Kurihara, Daniel P Regan, Jonathan W Coy, Tracy L Webb, Steven W Dow
Mesenchymal stem cells (MSC) represent a readily accessible source of cells with potent immune modulatory activity. MSC can suppress ongoing inflammatory responses by suppressing T cell function, while fewer studies have examined the impact of MSC on dendritic cell function. The dog spontaneous disease model represents an important animal model with which to evaluate the safety and effectiveness of cellular therapy with MSC. This study evaluated the effects of canine MSC on the activation and maturation of canine monocyte-derived dendritic cells (DC), as well as mechanisms underlying these effects...
November 14, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27824291/development-of-a-micronized-meniscus-extracellular-matrix-scaffold-for-potential-augmentation-of-meniscus-repair-and-regeneration
#6
Farrah A Monibi, Chantelle C Bozynski, Keiichi Kuroki, Aaron M Stoker, Ferris Pfeiffer, Seth L Sherman, James Lee Cook
Decellularized scaffolds composed of extracellular matrix (ECM) hold promise for repair and regeneration of the meniscus, given the potential for ECM-based biomaterials to aid in stem cell recruitment, infiltration, and differentiation. The objectives of this study were to decellularize canine menisci in order to fabricate a micronized, ECM-derived scaffold, and to determine the cytocompatibility and repair potential of the scaffold ex vivo. Menisci were decellularized with a combination of physical agitation and chemical treatments...
November 8, 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27816649/engraftment-efficiency-after-intra-bone-marrow-versus-intravenous-transplantation-of-bone-marrow-cells-in-a-canine-nonmyeloablative-dla-identical-transplantation-model
#7
Sandra Lange, Anne Steder, Doreen Killian, Gudrun Knuebel, Anett Sekora, Heike Vogel, Iris Lindner, Simone Dunkelmann, Friedrich Prall, Hugo Murua Escobar, Mathias Freund, Christian Junghanss
An intra-bone marrow (IBM) hematopoietic stem cell transplantation (HSCT) is assumed to optimize the homing process and therefore to improve engraftment as well as hematopoietic recovery compared with conventional i.v. HSCT. This study investigated the feasibility and efficacy of IBM HSCT after nonmyeloablative conditioning in an allogeneic canine HSCT model. Two study cohorts received IBM HSCT of either density gradient (IBM-I, n = 7) or buffy coat (IBM-II, n = 6) enriched bone marrow cells. An historical i...
November 2, 2016: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/27810198/canine-mesenchymal-stem-cells-are-neurotrophic-and-angiogenic-an-in-vitro-assessment-of-their-paracrine-activity
#8
I R Al Delfi, J J Sheard, C R Wood, A Vernallis, J F Innes, P Myint, W E B Johnson
Mesenchymal stem cells (MSCs) have been used in cell replacement therapies for connective tissue damage, but also can stimulate wound healing through paracrine activity. In order to further understand the potential use of MSCs to treat dogs with neurological disorders, this study examined the paracrine action of adipose-derived canine MSCs on neuronal and endothelial cell models. The culture-expanded MSCs exhibited a MSC phenotype according to plastic adherence, cell morphology, CD profiling and differentiation potential along mesenchymal lineages...
November 2016: Veterinary Journal
https://www.readbyqxmd.com/read/27779470/tumour-associated-macrophages-influence-canine-mammary-cancer-stem-like-cells-enhancing-their-pro-angiogenic-properties
#9
A Rybicka, C Eyileten, B Taciak, J Mucha, K Majchrzak, E Hellmen, M Krol
Cancer stem-like cells as cells with ability to self-renewal and potential to differentiate into various types of cells are known to be responsible for tumour initiation, recurrence and drug resistance. Hence a comprehensive research is concentrated on discovering cancer stem-like cells biology and interdependence between them and other cells. The aim of our study was to evaluate the impact of macrophages on cancer stem-like cells in canine mammary carcinomas. As recent studies indicated presence of macrophages in cancer environment stimulates cancer cells into more motile and invasive cells by acquisition of macrophage phenotypes...
August 2016: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/27774720/immunolocalization-of-proteins-in-the-spermatogenesis-process-of-canine
#10
Ncg Pieri, A F Souza, Acf Mançanares, Kcs Roballo, J B Casals, C E Ambrosio, D S Martins
Spermatogenesis is a process in which differentiated cells are produced and the adult stem cell population-known as spermatogonial stem cells (SSCs)-is continuously replenished. However, the molecular mechanisms underlying these processes are not fully understood in the canine species. We addressed this in this study by analysing the expression of specific markers in spermatogonia of seminiferous tubules of canine testes. SSCs at different stages of reproductive development (prepubertal and adult) were examined by immunohistochemistry and flow cytometry...
October 23, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27774699/characterization-of-teratogenic-potential-and-gene-expression-in-canine-and-feline-amniotic-membrane-derived-stem-cells
#11
M T Cardoso, A O Pinheiro, A S Vidane, J B Casals, V C de Oliveira, Njn Gonçalves, D S Martins, C E Ambrósio
The biosafety of innovative procedures that utilize stem cells in regenerative medicine has been addressed in several studies. Previous work has showed no tumour formation following the use of feline and human amniotic membrane-derived stem cells (AMSCs). In contrast, tumour formation was observed when canine AMSCs were utilized. These findings suggested that feline and human, but not canine, AMSCs are suitable for cell transplantation trials. This study aimed to further evaluate the feasibility of utilizing canine AMSCs for transplantation purposes as well as for felines...
October 23, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27733685/microvesicle-mediated-wnt-%C3%AE-catenin-signaling-promotes-interspecies-mammary-stem-progenitor-cell-growth
#12
Leen Bussche, Gat Rauner, Marc Antonyak, Bethany Syracuse, Melissa McDowell, Anthony M C Brown, Richard A Cerione, Gerlinde R Van de Walle
Signaling mechanisms that regulate mammary stem/progenitor cell (MaSC) self-renewal are essential for developmental changes that occur in the mammary gland during pregnancy, lactation, and involution. We observed that equine MaSCs (eMaSCs) maintain their growth potential in culture for an indefinite period, whereas canine MaSCs (cMaSCs) lose their growth potential in long term cultures. We then used this system to investigate the role of microvesicles (MVs) in promoting self-renewal properties. We found that Wnt3a and Wnt1 were expressed at higher levels in MVs isolated from eMaSCs compared with those from cMaSCs...
November 18, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27725063/effect-of-gelatin-on-osteogenic-cell-sheet-formation-using-canine-adipose-tissue-derived-mesenchymal-stem-cells
#13
Ah Young Kim, Yongsun Kim, Seung Hoon Lee, Yongseok Yoon, Wan-Hee Kim, Oh-Kyeong Kweon
Osteogenically differentiated cell sheet techniques using mesenchymal stem cells (MSCs) are available to stimulate bone regeneration. The advantage of cell sheet technique is delivering live cells effectively into the focal region. We developed a novel osteogenic cell sheet technique by adding gelatin to osteogenic cell medium. Gelatin-induced cell sheets (GCSs) were compared to conventional osteogenic cell sheets (OCSs). Undifferentiated MSCs (UCs) were used as a control. The morphology of these cell sheets was evaluated microscopically and histologically...
October 7, 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27701459/monoclonal-antibodies-13a4-and-ac133-do-not-recognize-the-canine-ortholog-of-mouse-and-human-stem-cell-antigen-prominin-1-cd133
#14
Kristina Thamm, Sylvi Graupner, Carsten Werner, Wieland B Huttner, Denis Corbeil
The pentaspan membrane glycoprotein prominin-1 (CD133) is widely used in medicine as a cell surface marker of stem and cancer stem cells. It has opened new avenues in stem cell-based regenerative therapy and oncology. This molecule is largely used with human samples or the mouse model, and consequently most biological tools including antibodies are directed against human and murine prominin-1. Although the general structure of prominin-1 including its membrane topology is conserved throughout the animal kingdom, its primary sequence is poorly conserved...
2016: PloS One
https://www.readbyqxmd.com/read/27698817/stem-cell-growth-factor-receptor-in-canine-vs-feline-osteosarcomas
#15
Birgitt Wolfesberger, Andrea Fuchs-Baumgartinger, Juraj Hlavaty, Florian R Meyer, Martin Hofer, Ralf Steinborn, Christiane Gebhard, Ingrid Walter
Osteosarcoma is considered the most common bone cancer in cats and dogs, with cats having a much better prognosis than dogs, since the great majority of dogs with osteosarcoma develop distant metastases. In search of a factor possibly contributing to this disparity, the stem cell growth factor receptor KIT was targeted, and the messenger (m)RNA and protein expression levels of KIT were compared in canine vs. feline osteosarcomas, as well as in normal bone. The mRNA expression of KIT was quantified by reverse transcription-quantitative polymerase chain reaction, and was observed to be significantly higher in canine (n=14) than in feline (n=5) osteosarcoma samples (P<0...
October 2016: Oncology Letters
https://www.readbyqxmd.com/read/27694153/effect-of-residual-bacteria-on-the-outcome-of-pulp-regeneration-in-vivo
#16
P Verma, A Nosrat, J R Kim, J B Price, P Wang, E Bair, H H Xu, A F Fouad
It is not known to what extent residual infection may interfere with the success of pulp regeneration procedures. The aim of this study was to determine, radiographically and histologically, the effect of residual bacteria on the outcome of pulp regeneration mediated by a tissue-engineered construct as compared with traditional revascularization. Periapical lesions were induced in 24 canine teeth of 6 ferrets. After disinfection with 1.25% NaOCl and triple antibiotic paste, ferret dental pulp stem cells, encapsulated in a hydrogel scaffold, were injected into half the experimental teeth...
September 30, 2016: Journal of Dental Research
https://www.readbyqxmd.com/read/27682609/a-comparative-study-of-the-effect-of-bio-oss-in-combination-with-concentrated-growth-factors-or-bone-marrow-derived-mesenchymal-stem-cells-in-canine-sinus-grafting
#17
Fang Wang, Qiong Li, Zuolin Wang
BACKGROUND: To compare the effects of Bio-Oss in combination with concentrated growth factors (CGFs) and bone marrow-derived mesenchymal stem cells (BMSCs) on bone regeneration for maxillary sinus floor augmentation in beagle dogs. METHODS: Six beagle dogs received bilateral maxillary sinus floor augmentation. Venous blood drawn from dogs was collected and centrifugated to obtain CGFs. BMSCs derived from canine bone marrow was cultured using density gradient centrifugation...
September 28, 2016: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/27681267/long-term-tolerance-towards-haploidentical-vascularized-composite-allograft-transplantation-in-a-canine-model-using-bone-marrow-or-mobilized-stem-cells
#18
Jeff Chang, Scott S Graves, Tiffany Butts-Miwongtum, George E Sale, Rainer Storb, David W Mathes
BACKGROUND: The development of safe and reliable protocols for the transplantation of the face and hands may be accomplished with animal modeling of transplantation of vascularized composite allografts (VCA). Previously, we demonstrated that tolerance to a VCA could be achieved after canine recipients were simultaneously given marrow from a dog leukocyte antigen (DLA) identical donor. In the present study, we extend those findings across a DLA mismatched barrier. METHODS: Eight Recipient dogs received total body irradiation (4...
September 27, 2016: Transplantation
https://www.readbyqxmd.com/read/27647656/hair-growth-promoting-effect-of-dermal-papilla-like-tissues-from-canine-adipose-derived-mesenchymal-stem-cells-through-vascular-endothelial-growth-factor
#19
Aeri Lee, Sohee Bae, Seung Hoon Lee, Oh-Kyeong Kweon, Wan Hee Kim
The purpose of this study was to investigate the protein expression pattern and the in vivo trichogenicity of dermal papilla like tissues (DPLTs) made from canine adipose-derived mesenchymal stem cells (ASCs) in athymic nude mice. Canine ASCs were isolated and cultured from adipose tissue, and differentiation was induced by culturing ASCs in dermal papilla forming media. DPLTs were embedded in collagen gel, and their structural characteristics and protein expression were evaluated by hematoxylin and eosin stain and immunohistochemistry...
September 16, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27637672/intravitreal-implantation-of-tpp1-transduced-stem-cells-delays-retinal-degeneration-in-canine-cln2-neuronal-ceroid-lipofuscinosis
#20
Christopher J Tracy, Rebecca E H Whiting, Jacqueline W Pearce, Baye G Williamson, Daniella P Vansteenkiste, Lauren E Gillespie, Leilani J Castaner, Jeffrey N Bryan, Joan R Coates, Cheryl A Jensen, Martin L Katz
The CLN2 form of neuronal ceroid lipofuscinosis is a neurodegenerative disease that results from mutations in the TPP1 gene. Affected children exhibit progressive declines in most neurological functions including vision. Functional declines are accompanied by progressive brain and retinal atrophy. TPP1 encodes the soluble lysosomal enzyme tripeptidyl peptidase-1 (TPP1). Dachshunds with a TPP1 null mutation exhibit a disorder very similar to human CLN2 disease. Periodic infusion of recombinant TPP1 protein or a single injection of a TPP1 gene therapy vector into the cerebrospinal fluid of affected dogs significantly delays the onset and progression of neurological signs but does not slow vision loss or retinal degeneration...
September 13, 2016: Experimental Eye Research
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