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equine stem cell

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https://www.readbyqxmd.com/read/28420427/isolation-and-characterization-of-equine-native-msc-populations
#1
Cristina L Esteves, Tara A Sheldrake, Simone P Mesquita, Juan J Pesántez, Timothy Menghini, Lucy Dawson, Bruno Péault, F Xavier Donadeu
BACKGROUND: In contrast to humans in which mesenchymal stem/stromal cell (MSC) therapies are still largely in the clinical trial phase, MSCs have been used therapeutically in horses for over 15 years, thus constituting a valuable preclinical model for humans. In human tissues, MSCs have been shown to originate from perivascular cells, namely pericytes and adventitial cells, which are identified by the presence of the cell surface markers CD146 and CD34, respectively. In contrast, the origin of MSCs in equine tissues has not been established, preventing the isolation and culture of defined cell populations in that species...
April 18, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28417917/minicircle-mediated-gene-delivery-to-canine-and-equine-mesenchymal-stem-cells
#2
Naomie Tidd, Jacob Michelsen, Bryan Hilbert, Jane C Quinn
Gene-directed tissue repair offers the clinician, human or veterinary, the chance to enhance cartilage regeneration and repair at a molecular level. Non-viral plasmid vectors have key biosafety advantages over viral vector systems for regenerative therapies due to their episomal integration however, conventional non-viral vectors can suffer from low transfection efficiency. Our objective was to identify and validate in vitro a novel non-viral gene expression vector that could be utilized for ex vivo and in vivo delivery to stromal-derived mesenchymal stem cells (MSCs)...
April 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28376684/equine-mesenchymal-stromal-cells-retain-a-pericyte-like-phenotype
#3
Cristina L Esteves, Tara A Sheldrake, Lucy Dawson, Timothy Menghini, Elisabeth Rink, Karin Amilon, Nusrat Khan, Bruno Peault, Xavier Donadeu
Mesenchymal Stem/Stromal Cells (MSCs) have been used in human and equine regenerative medicine, and interest in exploiting their potential has increased dramatically over the years. Despite significant effort to characterize equine MSCs, the actual origin of these cells and how much of their native phenotype is maintained in culture have not been determined. In this study, we investigated the relationship between MSCs, derived from adipose tissue (AT) and bone marrow (BM), and pericytes in the horse. Both pericyte (CD146, NG2 and αSMA) and MSC (CD29, CD90 and CD73) markers were detected in equine adipose tissue and co-localized around blood vessels...
April 4, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28328147/effect-of-needle-diameter-on-the-viability-of-equine-bone-marrow-derived-mesenchymal-stem-cells
#4
Hayley M Lang, Lauren V Schnabel, Jennifer M Cassano, Lisa A Fortier
OBJECTIVES: Mesenchymal stem cells (MSCs) are frequently delivered via needle injection for treatment of musculoskeletal injuries. The purpose of this study was to evaluate the effect of needle diameter on the viability of MSCs. METHODS: Equine bone marrow-derived MSCs from 5 horses were suspended in PBS, and held at room temperature for 7 hours to mimic shipping conditions. Two replicate samples for each needle size (20, 22, 23, or 25-gauge [ga]) were aspirated into a 3 mL syringe and re-injected into the holding vial 3 times, to reproduce the resuspension of cells prior to injection in clinical cases...
March 22, 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/28241885/repeated-intra-articular-injection-of-allogeneic-mesenchymal-stem-cells-causes-an-adverse-response-compared-to-autologous-cells-in-the-equine-model
#5
Amanda-Jo Joswig, Alexis Mitchell, Kevin J Cummings, Gwendolyn J Levine, Carl A Gregory, Roger Smith, Ashlee E Watts
BACKGROUND: Intra-articular injection of mesenchymal stem cells (MSCs) is efficacious in osteoarthritis therapy. A direct comparison of the response of the synovial joint to intra-articular injection of autologous versus allogeneic MSCs has not been performed. The objective of this study was to assess the clinical response to repeated intra-articular injection of allogeneic versus autologous MSCs prepared in a way to minimize xeno-contaminants in a large animal model. METHODS: Intra-articular injections of bone marrow-derived, culture-expanded MSCs to a forelimb metacarpophalangeal joint were performed at week 0 and week 4 (six autologous; six autologous with xeno-contamination; six allogeneic)...
February 28, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28222255/stemness-signature-of-equine-marrow-derived-mesenchymal-stem-cells
#6
Morteza Zahedi, Abbas Parham, Hesam Dehghani, Hossein Kazemi Mehrjerdi
Background: Application of competent cells such as mesenchymal stem cells (MSCs) for treatment of musculoskeletal disorders in equine athletes is increasingly needed. Moreover, similarities of horse and human in size, load and types of joint injuries, make horse as a good model for MSCs therapy studies. This study was designed to isolate and characterize stemness signature of equine bone marrow-derived mesenchymal stem cells (BM-MSCs). Methods: BM of three mares was aspirated and the mononuclear cells (MNCs) were isolated using density gradient...
February 28, 2017: International Journal of Stem Cells
https://www.readbyqxmd.com/read/28095754/3d-co-culture-of-meniscal-cells-and-mesenchymal-stem-cells-in-collagen-type-i-hydrogel-on-a-small-intestinal-matrix-a-pilot-study-towards-equine-meniscus-tissue-engineering
#7
Antje Kremer, Iris Ribitsch, Jenny Reboredo, Julia Dürr, Monika Egerbacher, Florien Jenner, Heike Walles
Meniscal injuries are the most frequently encountered soft tissue injuries in the equine stifle joint. Due to the inherent limited repair potential of meniscal tissue, meniscal injuries do not only affect the meniscus itself but also lead to impaired joint homeostasis and secondary osteoarthritis. The presented study compares 3D co-culture constructs of primary equine mesenchymal stem cells (MSC) and meniscus cells (MC) seeded on three different scaffolds - a cell-laden collagen type I hydrogel (Col I gel), a tissue-derived small intestinal matrix scaffold (SIS-muc) and a combination thereof - for their qualification to be applied for meniscus tissue engineering...
January 18, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27958776/autologous-and-allogeneic-equine-mesenchymal-stem-cells-exhibit-equivalent-immunomodulatory-properties-in-vitro
#8
Aimée C Colbath, Steven W Dow, Jennifer N Phillips, C Wayne McIlwraith, Laurie R Goodrich
The use of allogeneic bone marrow-derived mesenchymal stem cells (BMDMSCs) may provide an effective alternative to autologous BMDMSCs for treatment of equine musculoskeletal injuries. However, concerns have been raised regarding the potential safety and effectiveness of allogeneic BMDMSCs. We conducted studies to assess the immunological properties of equine allogeneic BMDMSCs compared with those of autologous BMDMSCs. For assessment of inherent immunogenicity, the relative ability of allogeneic and autologous BMDMSCs to stimulate spontaneous proliferation of equine lymphocytes was compared...
April 1, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/27931511/myod-overexpressed-equine-adipose-derived-stem-cells-enhanced-myogenic-differentiation-potential
#9
Soo-Eun Sung, Meeyul Hwang, Ah-Young Kim, Eun-Mi Lee, Eun-Joo Lee, Su-Kyeong Hwang, Shin-Yoon Kim, Hong-Kyun Kim, Kyu-Shik Jeong
Mesenchymal stem cells could potentially be used in the clinical treatment of muscle disorders and muscle regeneration. Adipose-derived stem cells (ADSCs) can be easily isolated from adipose tissue, as opposed to stem cells of other tissues. We believe that cell therapy using ADSCs could be applied to muscle disorders in horses and other species. We sought to improve the myogenic differentiation potential of equine ADSCs (eqADSCs) using a MyoD lentiviral vector. MyoD lentiviruses were transduced into eqADSCs and selected using puromycin...
November 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27931510/generation-of-equine-induced-pluripotent-stem-cells-and-analysis-of-their-therapeutic-potential-for-muscle-injuries
#10
Eun-Mi Lee, Ah-Young Kim, Eun-Joo Lee, Jin-Kyu Park, Se-Il Park, Ssang-Goo Cho, Hong Kyun Kim, Shin-Yoon Kim, Kyu-Shik Jeong
Horse health has become a major concern with the expansion of horse-related industries and sports; the importance of healthy muscles for horse performance and daily activities is undisputed. Here we generated equine-induced pluripotent stem cells (E-iPSCs) by reprogramming equine adipose-derived stem cells (E-ADSCs) into iPSCs using a polycistronic lentiviral vector encoding four transcription factors (i.e., Oct4, Sox2, Klf4, and c-Myc) and then examined their pluripotent characteristics. Subsequently, established E-iPSCs were transplanted into muscle-injured Rag/mdx mice...
November 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27927230/molecular-control-of-nitric-oxide-synthesis-through-enos-and-caveolin-1-interaction-regulates-osteogenic-differentiation-of-adipose-derived-stem-cells-by-modulation-of-wnt-%C3%AE-catenin-signaling
#11
Nadeeka Bandara, Saliya Gurusinghe, Shiang Yong Lim, Haying Chen, Shuangfeng Chen, Dawei Wang, Bryan Hilbert, Le-Xin Wang, Padraig Strappe
BACKGROUND: Nitric oxide (NO) plays a role in a number of physiological processes including stem cell differentiation and osteogenesis. Endothelial nitric oxide synthase (eNOS), one of three NO-producing enzymes, is located in a close conformation with the caveolin-1 (CAV-1(WT)) membrane protein which is inhibitory to NO production. Modification of this interaction through mutation of the caveolin scaffold domain can increase NO release. In this study, we genetically modified equine adipose-derived stem cells (eASCs) with eNOS, CAV-1(WT), and a CAV-1(F92A) (CAV-1(WT) mutant) and assessed NO-mediated osteogenic differentiation and the relationship with the Wnt signaling pathway...
December 7, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27925007/viability-in-vitro-differentiation-and-molecular-characterization-of-equine-adipose-tissue-derived-mesenchymal-stem-cells-cryopreserved-in-serum-and-serum-free-medium
#12
B Merlo, S Pirondi, E Iacono, B Rossi, F Ricci, G Mari
BACKGROUND: The effect of freezing-thawing equine adipose tissue-derived mesenchymal stem cells (eATMSCs) have been poorly investigated. OBJECTIVE: This study is to test the influence of cryopreservation solution and temperature when adding the cryoprotectant for freezing eATMSCs, and to investigate the effects of cryopreservation on their stemness features. MATERIALS AND METHODS: Four freezing protocols were evaluated. Viability and proliferation ability of cryopreserved cells were investigated by MTT assay...
July 2016: Cryo Letters
https://www.readbyqxmd.com/read/27899062/scleraxis-is-essential-for-tendon-differentiation-by-equine-embryonic-stem-cells-and-in-equine-fetal-tenocytes
#13
Emma P Bavin, Francesca Atkinson, Tom Barsby, Debbie J Guest
The transcription factor scleraxis is required for tendon development and is upregulated during embryonic stem cell (ESC) differentiation into tenocytes. However, its role beyond early embryonic development is not defined. We utilized a short hairpin RNA to knock down scleraxis expression in ESCs and adult and fetal tenocytes. No effect on growth or morphology was observed in two-dimensional cultures. However, scleraxis knockdown in fetal tenocytes significantly reduced COL1A1, COMP, and SOX9 gene expression...
March 15, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/27888544/conditioned-medium-a-new-alternative-for-cryopreservation-of-equine-umbilical-cord-mesenchymal-stem-cells
#14
Leandro Maia, Marianne Camargos Dias, Carolina Nogueira de Moraes, Camila de Paula Freitas-Dell'Aqua, Ligia S L Silveira da Mota, Valquíria Santiloni, Fernanda da Cruz Landim-Alvarenga
Cryopreservation is a feasible alternative to maintaining several cell lines, particularly for immediate therapeutic use, transportation of samples, and implementation of new in vitro studies. This work parts from the hypothesis that the medium of cryopreservation composed by 90% of conditioned medium (CM) supports cryopreservation of equine umbilical cord intervascular matrix mesenchymal stem cells (UCIM-MSCs), allowing the maintenance of the biological properties for the establishment of cell banks intended for therapeutic use and in vitro studies...
March 2017: Cell Biology International
https://www.readbyqxmd.com/read/27863532/microvesicles-secreted-from-equine-amniotic-derived-cells-and-their-potential-role-in-reducing-inflammation-in-endometrial-cells-in-an-in-vitro-model
#15
Claudia Perrini, Maria Giuseppina Strillacci, Alessandro Bagnato, Paola Esposti, Maria Giovanna Marini, Bruna Corradetti, Davide Bizzaro, Antonella Idda, Sergio Ledda, Emanuele Capra, Flavia Pizzi, Anna Lange-Consiglio, Fausto Cremonesi
BACKGROUND: It is known that a paracrine mechanism exists between mesenchymal stem cells and target cells. This process may involve microvesicles (MVs) as an integral component of cell-to-cell communication. METHODS: In this context, this study aims to understand the efficacy of MVs in in-vitro endometrial stressed cells in view of potential healing in in-vivo studies. For this purpose, the presence and type of MVs secreted by amniotic mesenchymal stem cells (AMCs) were investigated and the response of endometrial cells to MVs was studied using a dose-response curve at different concentrations and times...
November 18, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27862236/allogeneic-major-histocompatibility-complex-mismatched-equine-bone-marrow-derived-mesenchymal-stem-cells-are-targeted-for-death-by-cytotoxic-anti-major-histocompatibility-complex-antibodies
#16
A K Berglund, L V Schnabel
BACKGROUND: Allogeneic mesenchymal stem cells (MSCs) are a promising cell source for treating musculoskeletal injuries in horses. Controversy exists, however, over whether major histocompatibility complex (MHC)-mismatched MSCs are recognised by the recipient immune system and targeted for death by a cytotoxic antibody response. OBJECTIVES: To determine if cytotoxic anti-MHC antibodies generated in vivo following MHC-mismatched MSC injections are capable of initiating complement-dependent cytotoxicity of MSCs...
November 10, 2016: Equine Veterinary Journal
https://www.readbyqxmd.com/read/27829446/erratum-to-viability-of-equine-mesenchymal-stem-cells-during-transport-and-implantation
#17
Elaine R Garvican, Sandra Cree, Lydia Bull, Roger K W Smith, Jayesh Dudhia
No abstract text is available yet for this article.
November 9, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27818457/isolation-and-characterization-of-equine-dental-pulp-stem-cells-derived-from-thoroughbred-wolf-teeth
#18
Shingo Ishikawa, Chie Horinouchi, Daiki Murata, Shota Matsuzaki, Kazuhiro Misumi, Yohei Iwamoto, Kenji Korosue, Seiji Hobo
Mesenchymal stem cells (MSCs) are adult multipotent stem cells that are capable of self-renewal and differentiation into multiple cell lineages. Methods for cell therapy using MSCs have been developed in equine medicine. Recently, human dental pulp stem cells (DPSCs) have drawn much attention owing to their trophic factor producing ability and minimally invasive collection methods. However, there have been no reports on equine dental pulp-derived cells (eDPCs). Therefore, the aim of this study was to isolate and characterize the eDPCs from discarded wolf teeth...
January 20, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27746821/mri-based-assessment-of-intralesional-delivery-of-bone-marrow-derived-mesenchymal-stem-cells-in-a-model-of-equine-tendonitis
#19
Alexandra Scharf, Shannon P Holmes, Merrilee Thoresen, Jennifer Mumaw, Alaina Stumpf, John Peroni
Ultrasound-guided intralesional injection of mesenchymal stem cells (MSCs) is held as the benchmark for cell delivery in tendonitis. The primary objective of this study was to investigate the immediate cell distribution following intralesional injection of MSCs. Unilateral superficial digital flexor tendon (SDFT) lesions were created in the forelimb of six horses and injected with 10 × 10(6) MSCs labeled with superparamagnetic iron oxide nanoparticles (SPIOs) under ultrasound guidance. Assays were performed to confirm that there were no significant changes in cell viability, proliferation, migration, or trilineage differentiation due to the presence of SPIOs...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27733784/evaluation-of-adipose-derived-stromal-vascular-fraction-from-the-lateral-tailhead-inguinal-region-and-mesentery-of-horses
#20
Garrett L Metcalf, Scott R McClure, Jesse M Hostetter, Rudy F Martinez, Chong Wang
Use of mesenchymal stem cells (MSCs) found in the stromal vascular fraction (SVF) of equine adipose tissue has promising applications for regenerative therapies. The most commonly used source of equine adipose tissue is the subcutaneous tailhead. The objective of this study was to compare 3 adipose depot sites in horses and determine the viability and cellular yield, capillary density, gene expression for selected markers, and colony-forming unit fibroblasts (CFU-Fs) in adipose tissue taken from these sites...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
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