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https://www.readbyqxmd.com/read/27899062/scleraxis-is-essential-for-tendon-differentiation-by-equine-embryonic-stem-cells-and-in-equine-fetal-tenocytes
#1
Emma Bavin, Francesca Atkinson, Tom Barsby, Debbie Guest
The transcription factor scleraxis is required for tendon development and is upregulated during embryonic stem cell (ESC) differentiation into tenocytes. However, its role beyond early embryonic development is not defined. We utilised a short hairpin RNA to knockdown scleraxis expression in ESCs, adult and fetal tenocytes. No effect on growth or morphology was observed in 2D cultures. However, scleraxis knockdown in fetal tenocytes significantly reduced COL1A1, COMP and SOX9 gene expression. Scleraxis knockdown in adult tenocytes had no effect on the expression of these genes...
November 29, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27888544/conditioned-medium-a-new-alternative-for-cryopreservation-of-equine-umbilical-cord-mesenchymal-stem-cells
#2
Leandro Maia, Marianne Camargos Dias, Carolina Nogueira de Moraes, Camila de Paula Freitas-Dell'aqua, Ligia S L Silveira da Mota, Valquíria Santiloni, Fernanda da Cruz Landim-Alvarenga
Cryopreservation is a feasible alternative to maintaining several cell lines, particularly for immediate therapeutic use, transportation of samples and implementation of new in vitro studies. This work parts from the hypothesis that the medium of cryopreservation composed by 90% of conditioned medium (CM) supports cryopreservation of equine umbilical cord intervascular matrix mesenchymal stem cells (UCIM-MSCs), allowing the maintenance of the biological properties for the establishment of cell banks intended for therapeutic use and in vitro studies...
November 26, 2016: Cell Biology International
https://www.readbyqxmd.com/read/27863532/microvesicles-secreted-from-equine-amniotic-derived-cells-and-their-potential-role-in-reducing-inflammation-in-endometrial-cells-in-an-in-vitro-model
#3
Claudia Perrini, Maria Giuseppina Strillacci, Alessandro Bagnato, Paola Esposti, Maria Giovanna Marini, Bruna Corradetti, Davide Bizzaro, Antonella Idda, Sergio Ledda, Emanuele Capra, Flavia Pizzi, Anna Lange-Consiglio, Fausto Cremonesi
BACKGROUND: It is known that a paracrine mechanism exists between mesenchymal stem cells and target cells. This process may involve microvesicles (MVs) as an integral component of cell-to-cell communication. METHODS: In this context, this study aims to understand the efficacy of MVs in in-vitro endometrial stressed cells in view of potential healing in in-vivo studies. For this purpose, the presence and type of MVs secreted by amniotic mesenchymal stem cells (AMCs) were investigated and the response of endometrial cells to MVs was studied using a dose-response curve at different concentrations and times...
November 18, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27862236/allogeneic-mhc-mismatched-equine-bone-marrow-derived-mesenchymal-stem-cells-are-targeted-for-death-by-cytotoxic-anti-mhc-antibodies
#4
A K Berglund, L V Schnabel
REASONS FOR PERFORMING STUDY: Allogeneic mesenchymal stem cells (MSCs) are a promising cell source for treating musculoskeletal injuries in horses. Controversy exists, however, over whether major histocompatibility complex (MHC)-mismatched MSCs are recognised by the recipient immune system and targeted for death by a cytotoxic antibody response. OBJECTIVES: To determine if cytotoxic anti-MHC antibodies generated in-vivo following MHC-mismatched MSC injections are capable of initiating complement-dependent cytotoxicity of MSCs...
November 10, 2016: Equine Veterinary Journal
https://www.readbyqxmd.com/read/27829446/erratum-to-viability-of-equine-mesenchymal-stem-cells-during-transport-and-implantation
#5
Elaine R Garvican, Sandra Cree, Lydia Bull, Roger K W Smith, Jayesh Dudhia
No abstract text is available yet for this article.
November 9, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27818457/isolation-and-characterization-of-equine-dental-pulp-stem-cells-derived-from-thoroughbred-wolf-teeth
#6
Shingo Ishikawa, Chie Horinouchi, Daiki Murata, Shota Matsuzaki, Kazuhiro Misumi, Yohei Iwamoto, Kenji Korosue, Seiji Hobo
Mesenchymal stem cells (MSCs) are adult multipotent stem cells that are capable of self-renewal and differentiation into multiple cell lineages. Methods for cell therapy using MSCs have been developed in equine medicine. Recently, human dental pulp stem cells (DPSCs) have drawn much attention owing to their trophic factor producing ability and minimally invasive collection methods. However, there have been no reports on equine dental pulp-derived cells (eDPCs). Therefore, the aim of this study was to isolate and characterize the eDPCs from discarded wolf teeth...
November 5, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27746821/mri-based-assessment-of-intralesional-delivery-of-bone-marrow-derived-mesenchymal-stem-cells-in-a-model-of-equine-tendonitis
#7
Alexandra Scharf, Shannon P Holmes, Merrilee Thoresen, Jennifer Mumaw, Alaina Stumpf, John Peroni
Ultrasound-guided intralesional injection of mesenchymal stem cells (MSCs) is held as the benchmark for cell delivery in tendonitis. The primary objective of this study was to investigate the immediate cell distribution following intralesional injection of MSCs. Unilateral superficial digital flexor tendon (SDFT) lesions were created in the forelimb of six horses and injected with 10 × 10(6) MSCs labeled with superparamagnetic iron oxide nanoparticles (SPIOs) under ultrasound guidance. Assays were performed to confirm that there were no significant changes in cell viability, proliferation, migration, or trilineage differentiation due to the presence of SPIOs...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27733784/evaluation-of-adipose-derived-stromal-vascular-fraction-from-the-lateral-tailhead-inguinal-region-and-mesentery-of-horses
#8
Garrett L Metcalf, Scott R McClure, Jesse M Hostetter, Rudy F Martinez, Chong Wang
Use of mesenchymal stem cells (MSCs) found in the stromal vascular fraction (SVF) of equine adipose tissue has promising applications for regenerative therapies. The most commonly used source of equine adipose tissue is the subcutaneous tailhead. The objective of this study was to compare 3 adipose depot sites in horses and determine the viability and cellular yield, capillary density, gene expression for selected markers, and colony-forming unit fibroblasts (CFU-Fs) in adipose tissue taken from these sites...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/27733685/microvesicle-mediated-wnt-%C3%AE-catenin-signaling-promotes-interspecies-mammary-stem-progenitor-cell-growth
#9
Leen Bussche, Gat Rauner, Marc Antonyak, Bethany Syracuse, Melissa McDowell, Anthony M C Brown, Richard A Cerione, Gerlinde R Van de Walle
Signaling mechanisms that regulate mammary stem/progenitor cell (MaSC) self-renewal are essential for developmental changes that occur in the mammary gland during pregnancy, lactation, and involution. We observed that equine MaSCs (eMaSCs) maintain their growth potential in culture for an indefinite period, whereas canine MaSCs (cMaSCs) lose their growth potential in long term cultures. We then used this system to investigate the role of microvesicles (MVs) in promoting self-renewal properties. We found that Wnt3a and Wnt1 were expressed at higher levels in MVs isolated from eMaSCs compared with those from cMaSCs...
November 18, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27732616/in-vitro-and-in-vivo-development-of-horse-cloned-embryos-generated-with-ipscs-mesenchymal-stromal-cells-and-fetal-or-adult-fibroblasts-as-nuclear-donors
#10
Ramiro Olivera, Lucia Natalia Moro, Roberto Jordan, Carlos Luzzani, Santiago Miriuka, Martin Radrizzani, F Xavier Donadeu, Gabriel Vichera
The demand for equine cloning as a tool to preserve high genetic value is growing worldwide; however, nuclear transfer efficiency is still very low. To address this issue, we first evaluated the effects of time from cell fusion to activation (<1h, n = 1261; 1-2h, n = 1773; 2-3h, n = 1647) on in vitro and in vivo development of equine embryos generated by cloning. Then, we evaluated the effects of using different nuclear donor cell types in two successive experiments: I) induced pluripotent stem cells (iPSCs) vs...
2016: PloS One
https://www.readbyqxmd.com/read/27729913/utility-of-a-mouse-model-of-osteoarthritis-to-demonstrate-cartilage-protection-by-ifn%C3%AE-primed-equine-mesenchymal-stem-cells
#11
Marie Maumus, Gautier Roussignol, Karine Toupet, Geraldine Penarier, Isabelle Bentz, Sandrine Teixeira, Didier Oustric, Mireille Jung, Olivier Lepage, Regis Steinberg, Christian Jorgensen, Danièle Noel
OBJECTIVE: Mesenchymal stem cells isolated from adipose tissue (ASC) have been shown to influence the course of osteoarthritis (OA) in different animal models and are promising in veterinary medicine for horses involved in competitive sport. The aim of this study was to characterize equine ASCs (eASCs) and investigate the role of interferon-gamma (IFNγ)-priming on their therapeutic effect in a murine model of OA, which could be relevant to equine OA. METHODS: ASC were isolated from subcutaneous fat...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27726447/exposure-of-a-tendon-extracellular-matrix-to-synovial-fluid-triggers-endogenous-and-engrafted-cell-death-a-mechanism-for-failed-healing-of-intrathecal-tendon-injuries
#12
Elaine R Garvican, Mazdak Salavati, Roger K W Smith, Jayesh Dudhia
AIM: The purpose of this study was to investigate the effect of normal synovial fluid (SF) on exposed endogenous tendon-derived cells (TDCs) and engrafted mesenchymal stem cells (MSCs) within the tendon extracellular matrix. METHODS: Explants from equine superficial digital flexor (extra-synovial) and deep digital flexor tendons (DDFTs) from the compressed, intra-synovial and the tensile, extra-synovial regions were cultured in allogeneic or autologous SF-media...
October 11, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27712399/priming-equine-bone-marrow-derived-mesenchymal-stem-cells-with-proinflammatory-cytokines-implications-in-immunomodulation-immunogenicity-balance-cell-viability-and-differentiation-potential
#13
Laura Barrachina, Ana Rosa Remacha, Antonio Romero, Francisco José Vázquez, Jorge Albareda, Marta Prades, Jaime Gosálvez, Rosa Roy, Pilar Zaragoza, Inmaculada Martín-Burriel, Clementina Rodellar
Mesenchymal stem cells (MSCs) have a great potential for treating equine musculoskeletal injuries. Although their mechanisms of action are not completely known, their immunomodulatory properties appear to be key in their functions. The expression of immunoregulatory molecules by MSCs is regulated by proinflammatory cytokines; so inflammatory priming of MSCs might improve their therapeutic potential. However, inflammatory environment could also increase MSC immunogenicity and decrease MSC viability and differentiation capacity...
November 3, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27695697/a-novel-model-for-acute-peripheral-nerve-injury-in-the-horse-and-evaluation-of-the-effect-of-mesenchymal-stromal-cells-applied-in-situ-on-nerve-regeneration-a-preliminary-study
#14
Claudia Cruz Villagrán, Jim Schumacher, Robert Donnell, Madhu S Dhar
Transplantation of mesenchymal stromal cells (MSCs) to sites of experimentally created nerve injury in laboratory animals has shown promising results in restoring nerve function. This approach for nerve regeneration has not been reported in horses. In this study, we first evaluated the in vitro ability of equine bone marrow-derived MSCs (EBM-MSCs) to trans-differentiate into Schwann-like cells and subsequently tested the MSCs in vivo for their potential to regenerate a transected nerve after implantation. The EBM-MSCs from three equine donors were differentiated into SCLs for 7 days, in vitro, in the presence of specialized differentiation medium and evaluated for morphological characteristics, by using confocal microscopy, and for protein characteristics, by using selected Schwann cell markers (GFAP and S100b)...
2016: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/27651133/evaluation-of-amniotic-mesenchymal-cell-derivatives-on-cytokine-production-in-equine-alveolar-macrophages-an-in-vitro-approach-to-lung-inflammation
#15
Enrica Zucca, Emanuela Corsini, Valentina Galbiati, Anna Lange-Consiglio, Francesco Ferrucci
BACKGROUND: Data obtained in both animal models and clinical trials suggest that cell-based therapies represent a potential therapeutic strategy for lung repair and remodeling. Recently, new therapeutic approaches based on the use of stem cell derivatives (e.g., conditioned medium (CM) and microvesicles (MVs)) to regenerate tissues and improve their functions were proposed. The aim of this study was to investigate the immunomodulatory effects of equine amniotic mesenchymal cell derivatives on lipopolysaccharide (LPS)-induced cytokine production in equine alveolar macrophages, which may be beneficial in lung inflammatory disorders such as recurrent airway obstruction (RAO) in horses...
September 20, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27629697/equine-metabolic-syndrome-impairs-adipose-stem-cells-osteogenic-differentiation-by-predominance-of-autophagy-over-selective-mitophagy
#16
Krzysztof Marycz, Katarzyna Kornicka, Monika Marędziak, Paweł Golonka, Jakub Nicpoń
Adipose-derived mesenchymal stem cells (ASC) hold great promise in the treatment of many disorders including musculoskeletal system, cardiovascular and/or endocrine diseases. However, the cytophysiological condition of cells, used for engraftment seems to be fundamental factor that might determine the effectiveness of clinical therapy. In this study we investigated growth kinetics, senescence, accumulation of oxidative stress factors, mitochondrial biogenesis, autophagy and osteogenic differentiation potential of ASC isolated from horses suffered from equine metabolic syndrome (EMS)...
September 14, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27572709/intramuscular-transplantation-of-allogeneic-mesenchymal-stromal-cells-derived-from-equine-umbilical-cord
#17
Marianne Camargos Dias, Fernanda da Cruz Landim-Alvarenga, Carolina Nogueira de Moraes, Leonardo Dourado da Costa, Caroline Medeiros Geraldini, Vânia Maria de Vasconcelos Machado, Leandro Maia
Background and Objectives: Mesenchymal stromal cells (MSCs) have great therapeutic potential, particularly in the process of tissue repair and immunomodulation through the secretion of biomolecules. Thus, the aim of this study was to evaluate the hypothesis that intramuscular transplantation of allogeneic MSCs obtained from equine umbilical cord (UC-MSCs) is safe, demonstrating that this is a suitable source of stem cells for therapeutic use. Methods and Results: For this, UC-MSCs were cultured, characterized and cryopreserved for future transplantation in six healthy mares...
November 30, 2016: International Journal of Stem Cells
https://www.readbyqxmd.com/read/27565870/molecular-characterization-of-equine-april-and-its-expression-analysis-during-the-adipogenic-differentiation-of-equine-adipose-derived-stem-cell-in-vitro
#18
Haitao Wu, Xiaolin Bi, Fang Cao, Cuicui Zhu, Hongzhen Liu, Jinyun Song, Lei Ma, Li Ma, Yi Zhang, Dongwei Zhao, Hongyan Liu, Xinzhou Xu, Shuangquan Zhang
A proliferation inducing ligand (APRIL) is a member of the TNF superfamily. It shares two receptors with B-cell activating factor (BAFF), B-cell maturation antigen (BCMA), and transmembrane activator and CAML interactor (TACI). Herein, the equine APRIL was identified from equine adipose-derived stem cell (ASC), and the protein expression of APRIL and its related molecules were detected during the adipogenic differentiation of equine ASC in vitro. The equine APRIL gene was located on chromosome 11, spans 1852 base pairs (bp)...
October 2016: Animal Biotechnology
https://www.readbyqxmd.com/read/27406327/differential-adhesion-selection-for-enrichment-of-tendon-derived-progenitor-cells-during-in-vitro-culture
#19
Sushmitha Durgam, Brooke Schuster, Anna Cymerman, Allison Stewart, Matthew Stewart
Preplating, a technique used to separate rapidly adherent fibroblasts from the less-adherent progenitor cells, has been used successfully to isolate skeletal muscle-derived stem cells. The objective of this study was to determine if preplating could also be applied to enrich tendon-derived progenitor cells (TDPCs) before monolayer expansion. Cell suspensions obtained by collagenase digestion of equine lateral digital extensor tendon were serially transferred into adherent plates every 12 h for 4 days. TDPC fractions obtained from initial (TPP0), third (TPP3), and seventh (TPP7) preplate were passaged twice and used for subsequent analyses...
August 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27379167/effects-of-hypoxia-and-chitosan-on-equine-umbilical-cord-derived-mesenchymal-stem-cells
#20
D J Griffon, J Cho, J R Wagner, C Charavaryamath, J Wei, A Wagoner Johnson
Chitosan opens new perspectives in regenerative medicine as it enhances the properties of mesenchymal stem cells (MSCs) through formation of spheroids. Hypoxia has also been proposed to enhance stemness and survival of MSCs after in vivo implantation. These characteristics are relevant to the development of an off-the-shelf source of allogenic cells for regenerative therapy of tendinopathies. Umbilical cord-derived MSCs (UCM-MSCs) offer an abundant source of immature and immunoprivileged stem cells. In this study, equine UCM-MSCs (eqUCM-MSCs) conditioned for 3 and 7 days on chitosan films at 5% oxygen were compared to eqUCM-MSCs under standard conditions...
2016: Stem Cells International
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