keyword
MENU ▼
Read by QxMD icon Read
search

equine stem cell

keyword
https://www.readbyqxmd.com/read/29439436/chondrogenic-differentiation-of-defined-equine-mesenchymal-stem-cells-derived-from-umbilical-cord-blood-for-use-in-cartilage-repair-therapy
#1
Mélanie Desancé, Romain Contentin, Lélia Bertoni, Tangni Gomez-Leduc, Thomas Branly, Sandrine Jacquet, Jean-Marc Betsch, Agnès Batho, Florence Legendre, Fabrice Audigié, Philippe Galéra, Magali Demoor
Cartilage engineering is a new strategy for the treatment of cartilage damage due to osteoarthritis or trauma in humans. Racehorses are exposed to the same type of cartilage damage and the anatomical, cellular, and biochemical properties of their cartilage are comparable to those of human cartilage, making the horse an excellent model for the development of cartilage engineering. Human mesenchymal stem cells (MSCs) differentiated into chondrocytes with chondrogenic factors in a biomaterial appears to be a promising therapeutic approach for direct implantation and cartilage repair...
February 10, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29421480/advanced-nutritional-and-stem-cells-approaches-to-prevent-equine-metabolic-syndrome
#2
REVIEW
Krzysztof Marycz, Izabela Michalak, Katarzyna Kornicka
Horses metabolic disorders have become an important problem of modern veterinary medicine. Pathological obesity, insulin resistance and predisposition toward laminitis are associated with Equine Metabolic Syndrome (EMS). Based on pathogenesis of EMS, dietary and cell therapy management may significantly reduce development of that disorder. Special attention has been paid to the diet supplementation with high bioavailability minerals and mesenchymal stem cells (MSC) which increase insulin sensitivity. In nutrition, there is a great interests in natural algae enriched in biosorption process with micro and macroelements...
January 31, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29389887/improvement-of-the-chondrocyte-specific-phenotype-upon-equine-bone-marrow-mesenchymal-stem-cell-differentiation-influence-of-culture-time-transforming-growth-factors-and-type-i-collagen-sirnas-on-the-differentiation-index
#3
Thomas Branly, Romain Contentin, Mélanie Desancé, Thibaud Jacquel, Lélia Bertoni, Sandrine Jacquet, Frédéric Mallein-Gerin, Jean-Marie Denoix, Fabrice Audigié, Magali Demoor, Philippe Galéra
Articular cartilage is a tissue characterized by its poor intrinsic capacity for self-repair. This tissue is frequently altered upon trauma or in osteoarthritis (OA), a degenerative disease that is currently incurable. Similar musculoskeletal disorders also affect horses and OA incurs considerable economic loss for the equine sector. In the view to develop new therapies for humans and horses, significant progress in tissue engineering has led to the emergence of new generations of cartilage therapy. Matrix-associated autologous chondrocyte implantation is an advanced 3D cell-based therapy that holds promise for cartilage repair...
February 1, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29342064/minimally-manipulated-bone-marrow-concentrate-compared-with-microfracture-treatment-of-full-thickness-chondral-defects-a-one-year-study-in-an-equine-model
#4
Constance R Chu, Lisa A Fortier, Ashley Williams, Karin A Payne, Taralyn M McCarrel, Megan E Bowers, Diego Jaramillo
BACKGROUND: Microfracture is commonly performed for cartilage repair but usually results in fibrocartilage. Microfracture augmented by autologous bone marrow concentrate (BMC) was previously shown to yield structurally superior cartilage repairs in an equine model compared with microfracture alone. The current study was performed to test the hypothesis that autologous BMC without concomitant microfracture improves cartilage repair compared with microfracture alone. METHODS: Autologous sternal bone marrow aspirate (BMA) was concentrated using a commercial system...
January 17, 2018: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/29329028/equine-allogeneic-chondrogenic-induced-mesenchymal-stem-cells-a-gcp-target-animal-safety-and-biodistribution-study
#5
S Y Broeckx, J H Spaas, K Chiers, L Duchateau, L Van Hecke, L Van Brantegem, M Dumoulin, A M Martens, F Pille
The safety of the intra-articular use of mesenchymal stem cells (MSCs) is scarcely reported. Therefore, the goal of this study was to investigate the safety of a single intra-articular injection with allogeneic chondrogenic induced MSCs combined with equine plasma (=the investigational product: IVP) compared to a saline (0.9% NaCl) placebo control (=control product: CP). Sixteen healthy experimental horses were randomly assigned to receive a single intra-articular injection with either the IVP (n=8) or the CP (n=8) in the left metacarpophalangeal joint...
December 27, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29287161/evaluation-of-equine-synovial-derived-extracellular-matrix-scaffolds-seeded-with-equine-synovial-derived-mesenchymal-stem-cells
#6
Nathalie A Reisbig, Hayam A Hussein, Erin Pinnell, Alicia L Bertone
OBJECTIVE To create a bioactive synovium scaffold by infusing decellularized synovial-derived extracellular matrix (synECM) with synovial-derived mesenchymal stem cells (synMSCs). SAMPLE Synovium from the femoropatellar and medial femorotibial joints of equine cadavers. PROCEDURES The synMSCs were cultured in monolayer and not treated or cotransduced to enhance expression of green fluorescent protein (GFP) and human bone morphogenetic protein (BMP)-2. The synECM was decellularized with 0.1% peracetic acid and then seeded with synMSCs (0...
January 2018: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/29249319/innate-immune-responses-of-equine-monocytes-cultured-in-equine-platelet-lysate
#7
Maria C Naskou, Natalie A Norton, Ian B Copland, Jacques Galipeau, John F Peroni
Platelet lysate (PL) has been extensively used for the laboratory expansion of human mesenchymal stem cells (MSC) in order to avoid fetal bovine serum (FBS) which has been associated with immune-mediated host reactions and transmission of bovine-origin microbial contaminants. Before suggesting the routine use of PL for MSC culture, we wanted to further investigate whether PL alone might trigger inflammatory responses when exposed to reactive white blood cells such as monocytes. Our objectives were to evaluate the inflammatory profile of equine monocytes cultured with equine PL (ePL) and to determine if ePL can modulate the expression of inflammatory cytokines in lipopolysaccharide (LPS)-stimulated monocytes...
January 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29249315/the-immunomodulatory-function-of-equine-mscs-is-enhanced-by-priming-through-an-inflammatory-microenvironment-or-tlr3-ligand
#8
Jennifer M Cassano, Lauren V Schnabel, Margaret B Goodale, Lisa A Fortier
Mesenchymal stem cells (MSCs) have the therapeutic potential to treat a variety of inflammatory and degenerative disease processes, however the effects of the tissue environment on MSCs have been overlooked. Our hypothesis was that the immunomodulatory function of MSCs would be impaired by TLR4 stimulation or exposure to inflammatory macrophages, whereas their immunosuppressive properties would be enhanced by TLR3 stimulation. MSCs were exposed to polyinosinic:polycytidylic acid (poly I:C) to stimulate TLR3 receptors or lipopolysaccharide (LPS) to stimulate TLR4 receptors...
January 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29207466/evaluation-of-poly-lactic-co-glycolic-acid-alone-or-in-combination-with-hydroxyapatite-on-human-periosteal-cells-bone-differentiation-and-in-sinus-lift-treatment
#9
Gabriele Ceccarelli, Rossella Presta, Saturnino Marco Lupi, Nefele Giarratana, Nora Bloise, Laura Benedetti, Maria Gabriella Cusella De Angelis, Ruggero Rodriguez Y Baena
Most recent advances in tissue engineering in the fields of oral surgery and dentistry have aimed to restore hard and soft tissues. Further improvement of these therapies may involve more biological approaches and the use of dental tissue stem cells in combination with inorganic/organic scaffolds. In this study, we analyzed the osteoconductivity of two different inorganic scaffolds based on poly (lactic-co-glycolic) acid alone (PLGA-Fisiograft) or in combination with hydroxyapatite (PLGA/HA-Alos) in comparison with an organic material based on equine collagen (PARASORB Sombrero) both in vitro and in vivo...
December 2, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29175012/osteogenic-differentiation-of-equine-adipose-tissue-derived-mesenchymal-stem-cells-using-cacl2
#10
Mohamed I Elashry, Nadine Baulig, Manuela Heimann, Caroline Bernhardt, Sabine Wenisch, Stefan Arnhold
Adipose tissue derived mesenchymal stem cells (ASCs) may be used to cure bone defects after osteogenic differentiation. In this study we tried to optimize osteogenic differentiation for equine ASCs using various concentrations of CaCl2 in comparison to the standard osteogenic protocol. ASCs were isolated from subcutaneous adipose tissue from mixed breed horses. The osteogenic induction protocols were (1) the standard osteogenic medium (OM) composed of dexamethasone, ascorbic acid and β-glycerol phosphate; (2) CaCl2 based protocol composed of 3, 5 and 7...
November 21, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29131717/in-vitro-effects-of-high-intensity-laser-photobiomodulation-on-equine-bone-marrow-derived-mesenchymal-stem-cell-viability-and-cytokine-expression
#11
Frances J Peat, Aimee C Colbath, Lori M Bentsen, Laurie R Goodrich, Melissa R King
OBJECTIVE: This study aimed to examine the influence of neodymium-doped yttrium aluminum garnet (Nd:YAG) laser irradiation on equine bone marrow-derived mesenchymal stem cell (MSC) viability, proliferation, and cytokine expression in vitro. BACKGROUND: Photobiomodulation of cells using monochromatic light is a technique designed to influence cellular processes. Previous studies have shown dose-dependent effects of low-level laser irradiation on cell proliferation and cytokine expression in a range of cell types and species...
November 13, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/29063737/allogeneic-stem-cells-alter-gene-expression-and-improve-healing-of-distal-limb-wounds-in-horses
#12
Jamie A Textor, Kaitlin C Clark, Naomi J Walker, Fabio A Aristizobal, Amir Kol, Sarah S LeJeune, Andrea Bledsoe, Arik Davidyan, Sarah N Gray, Laurie K Bohannon-Worsley, Kevin D Woolard, Dori L Borjesson
Distal extremity wounds are a significant clinical problem in horses and humans and may benefit from mesenchymal stem cell (MSC) therapy. This study evaluated the effects of direct wound treatment with allogeneic stem cells, in terms of gross, histologic, and transcriptional features of healing. Three full-thickness cutaneous wounds were created on each distal forelimb in six healthy horses, for a total of six wounds per horse. Umbilical cord-blood derived equine MSCs were applied to each wound 1 day after wound creation, in one of four forms: (a) normoxic- or (b) hypoxic-preconditioned cells injected into wound margins, or (c) normoxic- or (d) hypoxic-preconditioned cells embedded in an autologous fibrin gel and applied topically to the wound bed...
October 24, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29049924/equine-mesenchymal-stem-cells-derived-from-endometrial-or-adipose-tissue-share-significant-biological-properties-but-have-distinctive-pattern-of-surface-markers-and-migration
#13
J Cabezas, D Rojas, F Navarrete, R Ortiz, G Rivera, F Saravia, L Rodriguez-Alvarez, F O Castro
Adult stromal mesenchymal stem cells (MSCs) have been postulated as responsible for cell renewal in highly and continuously regenerative tissues such as the endometrium. MSCs have been identified in the endometrium of many species including humans, rodents, pets and some farm animals, but not in horses. The objective of this work was to isolate such cells from the endometrium of mares and to compare their main biological attributes with horse adipose-derived MSCs. Here we successfully isolated and characterized endometrial MSCs (eMSCs) from mares...
October 6, 2017: Theriogenology
https://www.readbyqxmd.com/read/29031416/zinc-silicate-mineral-coated-scaffold-improved-in-vitro-osteogenic-differentiation-of-equine-adipose-derived-mesenchymal-stem-cells
#14
Behnaz Bageshlooyafshar, Saeid Vakilian, Mousa Kehtari, Tarlan Eslami-Arshaghi, Farjad Rafeie, Rouhallah Ramezanifard, Reza Rahchamani, Abdollah Mohammadi-Sangcheshmeh, Yousef Mostafaloo, Ehsan Seyedjafari
In current study we aimed to coat the PLLA scaffold with zinc (Zn) silicate mineral nanoparticles. Then, using equine adipose-derived stem cells (ASCs) we intended to compare the osteogenic induction potency of Zn silicate mineral-coated PLLA scaffold with uncoated PLLA scaffold and tissue culture plastic (TCPS). Adipose tissues were collected from 3 horses, and isolation of ASCs was achieved by enzymatic digestion. PLLA scaffold was successfully prepared using a phase separation method and coated with Zn silicate mineral nanoparticles...
September 18, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29019941/the-contribution-of-adipose-tissue-derived-mesenchymal-stem-cells-and-platelet-rich-plasma-to-the-treatment-of-chronic-equine-laminitis-a-proof-of-concept
#15
Mario Angelone, Virna Conti, Cristiano Biacca, Beatrice Battaglia, Laura Pecorari, Francesco Piana, Giacomo Gnudi, Fabio Leonardi, Roberto Ramoni, Giuseppina Basini, Silvia Dotti, Sabrina Renzi, Maura Ferrari, Stefano Grolli
Laminitis, a highly debilitating disease of the foot in ungulates, is characterized by pathological changes of the complex lamellar structures that maintain the appendicular skeleton within the hoof. Laminitis is a multifactorial disease that involves perturbation of the vascular, hematological, and inflammatory homeostasis of the foot. Interestingly, the pathogenesis of the disease resembles what is observed in metabolic syndromes and sepsis-induced organ failure in humans and animals. We hypothesized that local administration of mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) might contribute to establishing an anti-inflammatory and pro-angiogenic environment, and could stimulate the injured tissue in order to restore its functional integrity...
October 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28945121/effects-of-autologous-stromal-cells-and-cytokines-on-differentiation-of-equine-bone-marrow-derived-progenitor-cells
#16
Ute E Schwab, Rebecca L Tallmadge, Mary Beth Matychak, M Julia B Felippe
OBJECTIVE To develop an in vitro system for differentiation of equine B cells from bone marrow hematopoietic progenitor cells on the basis of protocols for other species. SAMPLE Bone marrow aspirates aseptically obtained from 12 research horses. PROCEDURES Equine bone marrow CD34 + cells were sorted by use of magnetic beads and cultured in medium supplemented with cytokines (recombinant human interleukin-7, equine interleukin-7, stem cell factor, and Fms-like tyrosine kinase-3), murine OP9 stromal cell preconditioned medium, and equine fetal bone marrow mesenchymal stromal cell preconditioned medium...
October 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28906588/surface-glycan-pattern-of-canine-equine-and-ovine-bone-marrow-derived-mesenchymal-stem-cells
#17
Salvatore Desantis, Gianluca Accogli, Antonio Crovace, Edda G Francioso, Alberto Maria Crovace
The use of bone marrow-derived mesenchymal stem cells (MSCs) for clinical and experimental studies is increasing, but full characterization of MSCs in veterinary species is hindered by the variability in species-specific cell surface marker expression and antibody cross reactivity. Recent studies demonstrated that the glycans in the glycocalyx of MSCs are promising candidates as cell biomarkers. In the present study, we analyzed the glycocalyx of canine MSCs (cMSCs), ovine MSCs (oMSCs), and equine MSCs (eMSCs) using a cell microarray procedure in which MSCs were spotted on microarray slides and incubated with a panel of 14 biotinylated lectins and Cy3-conjugated streptavidin...
September 14, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28906257/double-printing-of-hyaluronic-acid-poly-glycidol-hybrid-hydrogels-with-poly-%C3%AE%C2%B5-caprolactone-for-msc-chondrogenesis
#18
Simone Stichler, Thomas Böck, Naomi Claire Paxton, Sarah Bertlein, Riccardo Levato, Verena Schill, Willi Smolan, Jos Malda, Joerg Tessmar, Torsten Blunk, Juergen Groll
This study investigates the use of allyl-functionalized poly(glycidol)s (P(AGE-co-G)) as cytocompatible cross-linker for thiol-functionalized hyaluronic acid (HA-SH) and the optimization of this hybrid hydrogel as bioink for 3D bioprinting. Chemical cross-linking of gels with 10 wt.% overall polymer concentration was achieved by UV-induced radical thiol-ene coupling between the thiol and allyl groups. Addition of unmodified high molecular weight HA (1.36 MDa) allowed tuning of the rheology for extrusion based bioprinting...
September 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/28865236/horse-hair-follicles-a-novel-dermal-stem-cell-source-for-equine-regenerative-medicine
#19
Jule K Michler, Aline Hillmann, Vuk Savkovic, Christoph K W Mülling
The easily accessible niche represented by skin and its appendages may serve as a promising source to complement modern regenerative medicine for horses. In humans and in animal models for human medicine, the hair follicle and its stem cell niches are well characterized. Since literature in this field of equine research is scarce, we sought to analyze cells of the dermal stem cell niche of the equine hair follicle morphologically and for a subset of markers useful for cell characterization via immunolabeling...
September 2, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28851519/bioactive-glass-ceramic-nanoparticles-coated-poly-l-lactic-acid-scaffold-improved-osteogenic-differentiation-of-adipose-stem-cells-in-equine
#20
Fatemeh Sadat Mahdavi, Abdolreza Salehi, Ehsan Seyedjafari, Abdollah Mohammadi-Sangcheshmeh, Abdolreza Ardeshirylajimi
Horses with big bone fractures have low chance to live mainly due to the lake of a proper treatment strategy. We believe that further attempts in equine bone tissue engineering will probably be required to meet all the needs for the lesion therapies. Therefore in this study we aimed to investigate the osteogenic differentiation capacity of equine adipose-derived stem cells (e-ASCs) on nano-bioactive glass (nBGs) coated poly(l-lactic acid) (PLLA) nanofibers scaffold (nBG-PLLA). Using electrospinning technique, PLLA scaffold was prepared successfully and coated with nBGs...
July 20, 2017: Tissue & Cell
keyword
keyword
99612
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"