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equine stem cell

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https://www.readbyqxmd.com/read/28095754/3d-co-culture-of-meniscal-cells-and-mesenchymal-stem-cells-in-collagen-type-i-hydrogel-on-a-small-intestinal-matrix-a-pilot-study-towards-equine-meniscus-tissue-engineering
#1
Antje Kremer, Iris Ribitsch, Jenny Reboredo, Julia Dürr, Monika Egerbacher, Florien Jenner, Heike Walles
Meniscal injuries are the most frequently encountered soft tissue injuries in the equine stifle joint. Due to the inherent limited repair potential of meniscal tissue, meniscal injuries do not only affect the meniscus itself but also lead to impaired joint homeostasis and secondary osteoarthritis. The presented study compares 3D co-culture constructs of primary equine mesenchymal stem cells (MSC) and meniscus cells (MC) seeded on three different scaffolds - a cell-laden collagen type I hydrogel (Col I gel), a tissue-derived small intestinal matrix scaffold (SIS-muc) and a combination thereof - for their qualification to be applied for meniscus tissue engineering...
January 18, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27958776/autologous-and-allogeneic-equine-mesenchymal-stem-cells-exhibit-equivalent-immunomodulatory-properties-in-vitro
#2
Aimée C Colbath, Steven W Dow, Jennifer N Phillips, C Wayne McIlwraith, Laurie R Goodrich
The use of allogeneic bone marrow-derived mesenchymal stem cells (BMDMSCs) may provide an effective alternative to autologous BMDMSCs for treatment of equine musculoskeletal injuries. However, concerns have been raised regarding the potential safety and effectiveness of allogeneic BMDMSCs. We conducted studies to assess the immunological properties of equine allogeneic BMDMSCs compared with those of autologous BMDMSCs. For assessment of inherent immunogenicity, the relative ability of allogeneic and autologous BMDMSCs to stimulate spontaneous proliferation of equine lymphocytes was compared...
January 12, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/27931511/myod-overexpressed-equine-adipose-derived-stem-cells-enhanced-myogenic-differentiation-potential
#3
Soo-Eun Sung, Meeyul Hwang, Ah-Young Kim, Eun-Mi Lee, Eun-Joo Lee, Su-Kyeong Hwang, Shin-Yoon Kim, Hong-Kyun Kim, Kyu-Shik Jeong
Mesenchymal stem cells could potentially be used in the clinical treatment of muscle disorders and muscle regeneration. Adipose-derived stem cells (ADSCs) can be easily isolated from adipose tissue, as opposed to stem cells of other tissues. We believe that cell therapy using ADSCs could be applied to muscle disorders in horses and other species. We sought to improve the myogenic differentiation potential of equine ADSCs (eqADSCs) using a MyoD lentiviral vector. MyoD lentiviruses were transduced into eqADSCs and selected using puromycin...
November 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27931510/generation-of-equine-induced-pluripotent-stem-cells-and-analysis-of-their-therapeutic-potential-for-muscle-injuries
#4
Eun-Mi Lee, Ah-Young Kim, Eun-Joo Lee, Jin-Kyu Park, Se-Il Park, Ssang-Goo Cho, Hong Kyun Kim, Shin-Yoon Kim, Kyu-Shik Jeong
Horse health has become a major concern with the expansion of horse-related industries and sports; the importance of healthy muscles for horse performance and daily activities is undisputed. Here we generated equine-induced pluripotent stem cells (E-iPSCs) by reprogramming equine adipose-derived stem cells (E-ADSCs) into iPSCs using a polycistronic lentiviral vector encoding four transcription factors (i.e., Oct4, Sox2, Klf4, and c-Myc) and then examined their pluripotent characteristics. Subsequently, established E-iPSCs were transplanted into muscle-injured Rag/mdx mice...
November 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27927230/molecular-control-of-nitric-oxide-synthesis-through-enos-and-caveolin-1-interaction-regulates-osteogenic-differentiation-of-adipose-derived-stem-cells-by-modulation-of-wnt-%C3%AE-catenin-signaling
#5
Nadeeka Bandara, Saliya Gurusinghe, Shiang Yong Lim, Haying Chen, Shuangfeng Chen, Dawei Wang, Bryan Hilbert, Le-Xin Wang, Padraig Strappe
BACKGROUND: Nitric oxide (NO) plays a role in a number of physiological processes including stem cell differentiation and osteogenesis. Endothelial nitric oxide synthase (eNOS), one of three NO-producing enzymes, is located in a close conformation with the caveolin-1 (CAV-1(WT)) membrane protein which is inhibitory to NO production. Modification of this interaction through mutation of the caveolin scaffold domain can increase NO release. In this study, we genetically modified equine adipose-derived stem cells (eASCs) with eNOS, CAV-1(WT), and a CAV-1(F92A) (CAV-1(WT) mutant) and assessed NO-mediated osteogenic differentiation and the relationship with the Wnt signaling pathway...
December 7, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27925007/viability-in-vitro-differentiation-and-molecular-characterization-of-equine-adipose-tissue-derived-mesenchymal-stem-cells-cryopreserved-in-serum-and-serum-free-medium
#6
B Merlo, S Pirondi, E Iacono, B Rossi, F Ricci, G Mari
BACKGROUND: The effect of freezing-thawing equine adipose tissue-derived mesenchymal stem cells (eATMSCs) have been poorly investigated. OBJECTIVE: This study is to test the influence of cryopreservation solution and temperature when adding the cryoprotectant for freezing eATMSCs, and to investigate the effects of cryopreservation on their stemness features. MATERIALS AND METHODS: Four freezing protocols were evaluated. Viability and proliferation ability of cryopreserved cells were investigated by MTT assay...
July 2016: Cryo Letters
https://www.readbyqxmd.com/read/27899062/scleraxis-is-essential-for-tendon-differentiation-by-equine-embryonic-stem-cells-and-in-equine-fetal-tenocytes
#7
Emma Bavin, Francesca Atkinson, Tom Barsby, Debbie Guest
The transcription factor scleraxis is required for tendon development and is upregulated during embryonic stem cell (ESC) differentiation into tenocytes. However, its role beyond early embryonic development is not defined. We utilised a short hairpin RNA to knockdown scleraxis expression in ESCs, adult and fetal tenocytes. No effect on growth or morphology was observed in 2D cultures. However, scleraxis knockdown in fetal tenocytes significantly reduced COL1A1, COMP and SOX9 gene expression. Scleraxis knockdown in adult tenocytes had no effect on the expression of these genes...
November 29, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27888544/conditioned-medium-a-new-alternative-for-cryopreservation-of-equine-umbilical-cord-mesenchymal-stem-cells
#8
Leandro Maia, Marianne Camargos Dias, Carolina Nogueira de Moraes, Camila de Paula Freitas-Dell'aqua, Ligia S L Silveira da Mota, Valquíria Santiloni, Fernanda da Cruz Landim-Alvarenga
Cryopreservation is a feasible alternative to maintaining several cell lines, particularly for immediate therapeutic use, transportation of samples and implementation of new in vitro studies. This work parts from the hypothesis that the medium of cryopreservation composed by 90% of conditioned medium (CM) supports cryopreservation of equine umbilical cord intervascular matrix mesenchymal stem cells (UCIM-MSCs), allowing the maintenance of the biological properties for the establishment of cell banks intended for therapeutic use and in vitro studies...
November 26, 2016: Cell Biology International
https://www.readbyqxmd.com/read/27863532/microvesicles-secreted-from-equine-amniotic-derived-cells-and-their-potential-role-in-reducing-inflammation-in-endometrial-cells-in-an-in-vitro-model
#9
Claudia Perrini, Maria Giuseppina Strillacci, Alessandro Bagnato, Paola Esposti, Maria Giovanna Marini, Bruna Corradetti, Davide Bizzaro, Antonella Idda, Sergio Ledda, Emanuele Capra, Flavia Pizzi, Anna Lange-Consiglio, Fausto Cremonesi
BACKGROUND: It is known that a paracrine mechanism exists between mesenchymal stem cells and target cells. This process may involve microvesicles (MVs) as an integral component of cell-to-cell communication. METHODS: In this context, this study aims to understand the efficacy of MVs in in-vitro endometrial stressed cells in view of potential healing in in-vivo studies. For this purpose, the presence and type of MVs secreted by amniotic mesenchymal stem cells (AMCs) were investigated and the response of endometrial cells to MVs was studied using a dose-response curve at different concentrations and times...
November 18, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27862236/allogeneic-major-histocompatibility-complex-mismatched-equine-bone-marrow-derived-mesenchymal-stem-cells-are-targeted-for-death-by-cytotoxic-anti-major-histocompatibility-complex-antibodies
#10
A K Berglund, L V Schnabel
BACKGROUND: Allogeneic mesenchymal stem cells (MSCs) are a promising cell source for treating musculoskeletal injuries in horses. Controversy exists, however, over whether major histocompatibility complex (MHC)-mismatched MSCs are recognised by the recipient immune system and targeted for death by a cytotoxic antibody response. OBJECTIVES: To determine if cytotoxic anti-MHC antibodies generated in vivo following MHC-mismatched MSC injections are capable of initiating complement-dependent cytotoxicity of MSCs...
November 10, 2016: Equine Veterinary Journal
https://www.readbyqxmd.com/read/27829446/erratum-to-viability-of-equine-mesenchymal-stem-cells-during-transport-and-implantation
#11
Elaine R Garvican, Sandra Cree, Lydia Bull, Roger K W Smith, Jayesh Dudhia
No abstract text is available yet for this article.
November 9, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27818457/isolation-and-characterization-of-equine-dental-pulp-stem-cells-derived-from-thoroughbred-wolf-teeth
#12
Shingo Ishikawa, Chie Horinouchi, Daiki Murata, Shota Matsuzaki, Kazuhiro Misumi, Yohei Iwamoto, Kenji Korosue, Seiji Hobo
Mesenchymal stem cells (MSCs) are adult multipotent stem cells that are capable of self-renewal and differentiation into multiple cell lineages. Methods for cell therapy using MSCs have been developed in equine medicine. Recently, human dental pulp stem cells (DPSCs) have drawn much attention owing to their trophic factor producing ability and minimally invasive collection methods. However, there have been no reports on equine dental pulp-derived cells (eDPCs). Therefore, the aim of this study was to isolate and characterize the eDPCs from discarded wolf teeth...
November 5, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27746821/mri-based-assessment-of-intralesional-delivery-of-bone-marrow-derived-mesenchymal-stem-cells-in-a-model-of-equine-tendonitis
#13
Alexandra Scharf, Shannon P Holmes, Merrilee Thoresen, Jennifer Mumaw, Alaina Stumpf, John Peroni
Ultrasound-guided intralesional injection of mesenchymal stem cells (MSCs) is held as the benchmark for cell delivery in tendonitis. The primary objective of this study was to investigate the immediate cell distribution following intralesional injection of MSCs. Unilateral superficial digital flexor tendon (SDFT) lesions were created in the forelimb of six horses and injected with 10 × 10(6) MSCs labeled with superparamagnetic iron oxide nanoparticles (SPIOs) under ultrasound guidance. Assays were performed to confirm that there were no significant changes in cell viability, proliferation, migration, or trilineage differentiation due to the presence of SPIOs...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27733784/evaluation-of-adipose-derived-stromal-vascular-fraction-from-the-lateral-tailhead-inguinal-region-and-mesentery-of-horses
#14
Garrett L Metcalf, Scott R McClure, Jesse M Hostetter, Rudy F Martinez, Chong Wang
Use of mesenchymal stem cells (MSCs) found in the stromal vascular fraction (SVF) of equine adipose tissue has promising applications for regenerative therapies. The most commonly used source of equine adipose tissue is the subcutaneous tailhead. The objective of this study was to compare 3 adipose depot sites in horses and determine the viability and cellular yield, capillary density, gene expression for selected markers, and colony-forming unit fibroblasts (CFU-Fs) in adipose tissue taken from these sites...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/27733685/microvesicle-mediated-wnt-%C3%AE-catenin-signaling-promotes-interspecies-mammary-stem-progenitor-cell-growth
#15
Leen Bussche, Gat Rauner, Marc Antonyak, Bethany Syracuse, Melissa McDowell, Anthony M C Brown, Richard A Cerione, Gerlinde R Van de Walle
Signaling mechanisms that regulate mammary stem/progenitor cell (MaSC) self-renewal are essential for developmental changes that occur in the mammary gland during pregnancy, lactation, and involution. We observed that equine MaSCs (eMaSCs) maintain their growth potential in culture for an indefinite period, whereas canine MaSCs (cMaSCs) lose their growth potential in long term cultures. We then used this system to investigate the role of microvesicles (MVs) in promoting self-renewal properties. We found that Wnt3a and Wnt1 were expressed at higher levels in MVs isolated from eMaSCs compared with those from cMaSCs...
November 18, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27732616/in-vitro-and-in-vivo-development-of-horse-cloned-embryos-generated-with-ipscs-mesenchymal-stromal-cells-and-fetal-or-adult-fibroblasts-as-nuclear-donors
#16
Ramiro Olivera, Lucia Natalia Moro, Roberto Jordan, Carlos Luzzani, Santiago Miriuka, Martin Radrizzani, F Xavier Donadeu, Gabriel Vichera
The demand for equine cloning as a tool to preserve high genetic value is growing worldwide; however, nuclear transfer efficiency is still very low. To address this issue, we first evaluated the effects of time from cell fusion to activation (<1h, n = 1261; 1-2h, n = 1773; 2-3h, n = 1647) on in vitro and in vivo development of equine embryos generated by cloning. Then, we evaluated the effects of using different nuclear donor cell types in two successive experiments: I) induced pluripotent stem cells (iPSCs) vs...
2016: PloS One
https://www.readbyqxmd.com/read/27729913/utility-of-a-mouse-model-of-osteoarthritis-to-demonstrate-cartilage-protection-by-ifn%C3%AE-primed-equine-mesenchymal-stem-cells
#17
Marie Maumus, Gautier Roussignol, Karine Toupet, Geraldine Penarier, Isabelle Bentz, Sandrine Teixeira, Didier Oustric, Mireille Jung, Olivier Lepage, Regis Steinberg, Christian Jorgensen, Danièle Noel
OBJECTIVE: Mesenchymal stem cells isolated from adipose tissue (ASC) have been shown to influence the course of osteoarthritis (OA) in different animal models and are promising in veterinary medicine for horses involved in competitive sport. The aim of this study was to characterize equine ASCs (eASCs) and investigate the role of interferon-gamma (IFNγ)-priming on their therapeutic effect in a murine model of OA, which could be relevant to equine OA. METHODS: ASC were isolated from subcutaneous fat...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27726447/exposure-of-a-tendon-extracellular-matrix-to-synovial-fluid-triggers-endogenous-and-engrafted-cell-death-a-mechanism-for-failed-healing-of-intrathecal-tendon-injuries
#18
Elaine R Garvican, Mazdak Salavati, Roger K W Smith, Jayesh Dudhia
AIM: The purpose of this study was to investigate the effect of normal synovial fluid (SF) on exposed endogenous tendon-derived cells (TDCs) and engrafted mesenchymal stem cells (MSCs) within the tendon extracellular matrix. METHODS: Explants from equine superficial digital flexor (extra-synovial) and deep digital flexor tendons (DDFTs) from the compressed, intra-synovial and the tensile, extra-synovial regions were cultured in allogeneic or autologous SF-media...
October 11, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27712399/priming-equine-bone-marrow-derived-mesenchymal-stem-cells-with-proinflammatory-cytokines-implications-in-immunomodulation-immunogenicity-balance-cell-viability-and-differentiation-potential
#19
Laura Barrachina, Ana Rosa Remacha, Antonio Romero, Francisco José Vázquez, Jorge Albareda, Marta Prades, Jaime Gosálvez, Rosa Roy, Pilar Zaragoza, Inmaculada Martín-Burriel, Clementina Rodellar
Mesenchymal stem cells (MSCs) have a great potential for treating equine musculoskeletal injuries. Although their mechanisms of action are not completely known, their immunomodulatory properties appear to be key in their functions. The expression of immunoregulatory molecules by MSCs is regulated by proinflammatory cytokines; so inflammatory priming of MSCs might improve their therapeutic potential. However, inflammatory environment could also increase MSC immunogenicity and decrease MSC viability and differentiation capacity...
January 1, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/27695697/a-novel-model-for-acute-peripheral-nerve-injury-in-the-horse-and-evaluation-of-the-effect-of-mesenchymal-stromal-cells-applied-in-situ-on-nerve-regeneration-a-preliminary-study
#20
Claudia Cruz Villagrán, Jim Schumacher, Robert Donnell, Madhu S Dhar
Transplantation of mesenchymal stromal cells (MSCs) to sites of experimentally created nerve injury in laboratory animals has shown promising results in restoring nerve function. This approach for nerve regeneration has not been reported in horses. In this study, we first evaluated the in vitro ability of equine bone marrow-derived MSCs (EBM-MSCs) to trans-differentiate into Schwann-like cells and subsequently tested the MSCs in vivo for their potential to regenerate a transected nerve after implantation. The EBM-MSCs from three equine donors were differentiated into SCLs for 7 days, in vitro, in the presence of specialized differentiation medium and evaluated for morphological characteristics, by using confocal microscopy, and for protein characteristics, by using selected Schwann cell markers (GFAP and S100b)...
2016: Frontiers in Veterinary Science
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