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equine stem cell

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https://www.readbyqxmd.com/read/29776607/differentiation-of-equine-bone-marrow-derived-mesenchymal-stem-cells-increases-the-expression-of-immunogenic-genes
#1
Laura Barrachina, Ana Rosa Remacha, Antonio Romero, Pilar Zaragoza, Francisco José Vázquez, Clementina Rodellar
Mesenchymal stem cells (MSCs) are a promising treatment for equine musculoskeletal injuries because of their ability to regulate the inflammation and to differentiate into other cell types. Since interest in allogeneic therapy is rising, concerns about MSC immunogenicity need to be addressed. Differentiated MSCs from several species increase their expression of immunogenic molecules and induce alloresponses, but equine MSC immunogenic profile after differentiation has not been reported. Therefore, the aim of this study was to assess the gene expression of immunogenic markers in tri-lineage differentiated equine bone marrow derived MSCs (eBM-MSCs)...
June 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29775846/reproductive-stage-and-sex-steroid-hormone-levels-influence-the-expression-of-mesenchymal-stromal-cell-msc-markers-in-the-equine-endometrium
#2
B Elisabeth Rink, Juliane Kuhl, Cristina L Esteves, Hilari M French, Elaine Watson, Christine Aurich, F Xavier Donadeu
Mesenchymal stem or stromal cells (MSCs) play key roles in tissue homeostasis. In the cyclic equine endometrium, this may be regulated by changes in serum concentrations of sex steroid hormones. This study was designed to investigate the changes in endometrial expression of MSC markers during reproductive cycles in mares and the influence of sex steroid hormones on endometrial MSC proliferation in vitro. Endometrial biopsies were collected from pony mares at different reproductive stages (estrus; day 5 and 13 after ovulation; seasonal anestrus; 20 h and 7days post-partum; n = 5 per stage) and were analyzed by RT-qPCR...
May 4, 2018: Theriogenology
https://www.readbyqxmd.com/read/29721754/effects-of-three-dimensional-spheroid-culture-on-equine-mesenchymal-stem-cell-plasticity
#3
Mi Jeong Park, Jienny Lee, Jeong Su Byeon, Da-Un Jeong, Na-Yeon Gu, In-Soo Cho, Sang-Ho Cha
Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy fields. We optimize culture conditions of equine adipose tissue-derived MSCs (eAD-MSCs) for treatment of horse fractures. To investigate enhancing properties of three-dimensional (3D) culture system in eAD-MSCs, we performed various sized spheroid formation and determined changes in gene expression levels to obtain different sized spheroid for cell therapy. eAD-MSCs were successfully isolated from horse tailhead. Using hanging drop method, spheroid formation was generated for three days...
May 2, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29715649/allogeneic-mesenchymal-stem-cell-transplantation-in-healthy-equine-superficial-digital-flexor-tendon-a-study-of-the-local-inflammatory-response
#4
Jaqueline Souza Brandão, Marina Landim Alvarenga, João Pedro Hubbe Pfeifer, Vitor Hugo Dos Santos, Carlos Eduardo Fonseca-Alves, Mirian Rodrigues, Renée Laufer-Amorim, José Antonio Lucas Castillo, Ana Liz Garcia Alves
The superficial digital flexor tendon (SDFT) is a structure frequently affected by injuries in high-performance athletic horses, and there are limited therapeutic options. Regenerative medicine has evolved significantly in treating different illnesses. However, understanding the cellular behaviour during mesenchymal stem cell (MSC) transplantation in healthy tissues is not fully known yet. To address the inflammatory response induced by allogeneic MSC transplantation, this study evaluated the local inflammatory response after the application of allogeneic adipose tissue-derived mesenchymal stem cells (AT-MSCs) in the equine tendon compared to an autologous transplant and the control group...
June 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29713634/cell-based-therapies-for-joint-disease-in-veterinary-medicine-what-we-have-learned-and-what-we-need-to-know
#5
REVIEW
Sophie Helen Bogers
Biological cell-based therapies for the treatment of joint disease in veterinary patients include autologous-conditioned serum, platelet-rich plasma, and expanded or non-expanded mesenchymal stem cell products. This narrative review outlines the processing and known mechanism of action of these therapies and reviews current preclinical and clinical efficacy in joint disease in the context of the processing type and study design. The significance of variation for biological activity and consequently regulatory approval is also discussed...
2018: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29685993/osteoblast-differentiation-of-equine-induced-pluripotent-stem-cells
#6
Arabella Baird, Timothy Lindsay, Alice Everett, Valentine Iyemere, Yasmin Z Paterson, Alyce McClellan, Frances M D Henson, Deborah J Guest
Bone fractures occur in horses following traumatic and non-traumatic (bone overloading) events. They can be difficult to treat due to the need for the horse to bear weight on all legs during the healing period. Regenerative medicine to improve fracture union and recovery could significantly improve horse welfare. Equine induced pluripotent stem cells (iPSCs) have previously been derived. Here we show that equine iPSCs cultured for 21 days in osteogenic induction media on an OsteoAssay surface upregulate the expression of osteoblast associated genes and proteins, including COL1A1 , SPARC , SPP1 , IBSP , RUNX2 and BGALP We also demonstrate that iPSC-osteoblasts are able to produce a mineralised matrix with both calcium and hydroxyapatite deposition...
May 10, 2018: Biology Open
https://www.readbyqxmd.com/read/29618647/identification-of-the-rna-pseudoknot-within-the-3-end-of-the-porcine-reproductive-and-respiratory-syndrome-virus-genome-as-a-pathogen-associated-molecular-pattern-to-activate-antiviral-signaling-via-rig-i-and-toll-like-receptor-3
#7
Sha Xie, Xin-Xin Chen, Songlin Qiao, Rui Li, Yangang Sun, Shuangfei Xia, Lin-Jian Wang, Xuegang Luo, Ruiguang Deng, En-Min Zhou, Gai-Ping Zhang
Once infected by viruses, cells can detect pathogen-associated molecular patterns (PAMPs) on viral nucleic acid by host pattern recognition receptors (PRRs) to initiate the antiviral response. Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of porcine reproductive and respiratory syndrome (PRRS), characterized by reproductive failure in sows and respiratory diseases in pigs of different ages. To date, the sensing mechanism of PRRSV has not been elucidated. Here, we reported that the pseudoknot region residing in the 3' untranslated regions (UTR) of the PRRSV genome, which has been proposed to regulate RNA synthesis and virus replication, was sensed as nonself by retinoic acid-inducible gene I (RIG-I) and Toll-like receptor 3 (TLR3) and strongly induced type I interferons (IFNs) and interferon-stimulated genes (ISGs) in porcine alveolar macrophages (PAMs)...
June 15, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29615127/inflammatory-licensed-equine-mscs-are-chondroprotective-and-exhibit-enhanced-immunomodulation-in-an-inflammatory-environment
#8
Jennifer M Cassano, Lauren V Schnabel, Margaret B Goodale, Lisa A Fortier
BACKGROUND: Inflammatory licensed mesenchymal stem cells (MSCs) have the ability to promote functional tissue repair. This study specifically sought to understand how the recipient tissue environment reciprocally affects MSC function. Inflammatory polarized macrophages, modeling an injured tissue environment, were exposed to licensed MSCs, and the resultant effects of MSC immunomodulation and functionality of the MSC secretome on chondrocyte homeostasis were studied. METHODS: Inflammatory licensed MSCs were generated through priming with either IFN-γ or polyinosinic:polycytidylic acid (poly I:C)...
April 3, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29608232/cytokine-induced-interleukin-1-receptor-antagonist-protein-expression-in-genetically-engineered-equine-mesenchymal-stem-cells-for-osteoarthritis-treatment
#9
Simone Gabner, Reinhard Ertl, Karsten Velde, Matthias Renner, Florien Jenner, Monika Egerbacher, Juraj Hlavaty
BACKGROUND: A combination of tissue engineering methods employing mesenchymal stem cells (MSCs) together with gene transfer takes advantage of innovative strategies and highlights a new approach for targeting osteoarthritis (OA) and other cartilage defects. Furthermore, the development of systems allowing tunable transgene expression as regulated by natural disease-induced substances is highly desirable. METHODS: Bone marrow-derived equine MSCs were transduced with a lentiviral vector expressing interleukin-1 receptor antagonist (IL-1Ra) gene under the control of an inducible nuclear factor-kappa B-responsive promoter and IL-1Ra production upon pro-inflammatory cytokine stimulation [tumor necrosis factor (TNF)α, interleukin (IL)-1β] was analysed...
May 2018: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29601969/concepts-and-challenges-in-the-use-of-mesenchymal-stem-cells-as-a-treatment-for-cartilage-damage-in-the-horse
#10
REVIEW
Mohammed Zayed, Steve Adair, Tena Ursini, James Schumacher, Nabil Misk, Madhu Dhar
Osteoarthritis (OA), the most common form of joint disease affecting humans and horses, is characterized by the advance and decline of cartilage and loss of function of the affected joint. The progression of OA is steadily accompanied with biochemical events, which interfere with the cytokines and proteolytic enzymes responsible for progress of the disease. Recently, regenerative therapies have been used with an assumption that mesenchymal stem cells (MSCs) possess the potential to prevent the advancement of cartilage damage and potentially regenerate the injured tissue with an ultimate goal of preventing OA...
June 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29587733/culture-of-mesenchymal-stem-cells-derived-from-equine-synovial-membrane-in-alginate-hydrogel-microcapsules
#11
Vitor Hugo Santos, João Pedro Hübbe Pfeifer, Jaqueline Brandão de Souza, Betsabéia Heloisa Gentilha Milani, Rogério Antonio de Oliveira, Marjorie Golim Assis, Elenice Deffune, Andrei Moroz, Ana Liz Garcia Alves
No abstract text is available yet for this article.
March 27, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29566772/platelet-lysate-as-a-novel-serum-free-media-supplement-for-the-culture-of-equine-bone-marrow-derived-mesenchymal-stem-cells
#12
Maria C Naskou, Scarlett M Sumner, Anna Chocallo, Hannah Kemelmakher, Merrilee Thoresen, Ian Copland, Jacques Galipeau, John F Peroni
BACKGROUND: Mesenchymal stem cells (MSCs) produced for clinical purposes rely on culture media containing fetal bovine serum (FBS) which is xenogeneic and has the potential to significantly alter the MSC phenotype, rendering these cells immunogenic. As a result of bovine-derived exogenous proteins expressed on the cell surface, MSCs may be recognized by the host immune system as non-self and be rejected. Platelet lysate (PL) may obviate some of these concerns and shows promising results in human medicine as a possible alternative to FBS...
March 22, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29562867/cortical-neurons-derived-from-equine-induced-pluripotent-stem-cells-are-susceptible-to-neurotropic-flavivirus-infection-and-replication-an-in-vitro-model-for-equine-neuropathic-diseases
#13
Patrick R J Fortuna, Helle Bielefeldt-Ohmann, Dmitry A Ovchinnikov, Ernst J Wolvetang, Deanne J Whitworth
Horses are susceptible to a number of neurotropic viruses, including West Nile virus (WNV), which is a pathogen of global significance in both horses and humans. However, there are no in vitro models with which to study infectious neuropathic diseases in the horse. In an effort to redress this, we have generated neurons from equine induced pluripotent stem cells (equiPSCs) that express a range of cortical neuron-specific markers, in addition to the membrane-bound ligand ephrin B3, which plays an important role in axon guidance as well as functioning as the receptor through which henipaviruses, such as Hendra virus, enter mammalian neurons...
May 15, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29535784/stem-cells-for-cartilage-repair-preclinical-studies-and-insights-in-translational-animal-models-and-outcome-measures
#14
REVIEW
Melissa Lo Monaco, Greet Merckx, Jessica Ratajczak, Pascal Gervois, Petra Hilkens, Peter Clegg, Annelies Bronckaers, Jean-Michel Vandeweerd, Ivo Lambrichts
Due to the restricted intrinsic capacity of resident chondrocytes to regenerate the lost cartilage postinjury, stem cell-based therapies have been proposed as a novel therapeutic approach for cartilage repair. Moreover, stem cell-based therapies using mesenchymal stem cells (MSCs) or induced pluripotent stem cells (iPSCs) have been used successfully in preclinical and clinical settings. Despite these promising reports, the exact mechanisms underlying stem cell-mediated cartilage repair remain uncertain. Stem cells can contribute to cartilage repair via chondrogenic differentiation, via immunomodulation, or by the production of paracrine factors and extracellular vesicles...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29508214/wharton-s-jelly-derived-mesenchymal-stem-cells-comparing-human-and-horse
#15
Barbara Merlo, Gabriella Teti, Eleonora Mazzotti, Laura Ingrà, Viviana Salvatore, Marina Buzzi, Giorgia Cerqueni, Manuela Dicarlo, Aliai Lanci, Carolina Castagnetti, Eleonora Iacono
Wharton's jelly (WJ) is an important source of mesenchymal stem cells (MSCs) both in human and other animals. The aim of this study was to compare human and equine WJMSCs. Human and equine WJMSCs were isolated and cultured using the same protocols and culture media. Cells were characterized by analysing morphology, growth rate, migration and adhesion capability, immunophenotype, differentiation potential and ultrastructure. Results showed that human and equine WJMSCs have similar ultrastructural details connected with intense synthetic and metabolic activity, but differ in growth, migration, adhesion capability and differentiation potential...
March 5, 2018: Stem Cell Reviews
https://www.readbyqxmd.com/read/29485180/progenitor-cells-in-auricular-cartilage-demonstrate-cartilage-forming-capacity-in-3d-hydrogel-culture
#16
I A Otto, R Levato, W R Webb, I M Khan, C C Breugem, J Malda
Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs). However, the quality of neo-tissues resulting from these cell types is inadequate due to inefficient chondrogenic differentiation and endochondral ossification, respectively...
February 27, 2018: European Cells & Materials
https://www.readbyqxmd.com/read/29478385/a-comparison-of-bone-marrow-and-cord-blood-mesenchymal-stem-cells-for-cartilage-self-assembly
#17
Jamie L White, Naomi J Walker, Jerry C Hu, Dori L Borjesson, Kyriacos A Athanasiou
Joint injury is a common cause of premature retirement for the human and equine athlete alike. Implantation of engineered cartilage offers the potential to increase the success rate of surgical intervention and hasten recovery times. Mesenchymal stem cells (MSCs) are a particularly attractive cell source for cartilage engineering. While bone marrow-derived MSCs (BM-MSCs) have been most extensively characterized for musculoskeletal tissue engineering, studies suggest that cord blood MSCs (CB-MSCs) may elicit a more robust chondrogenic phenotype...
April 2, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29454522/genetic-and-subunit-vaccines-based-on-the-stem-domain-of-the-equine-influenza-hemagglutinin-provide-homosubtypic-protection-against-heterologous-strains
#18
Lorena Itatí Ibañez, Cecilia Andrea Caldevilla, Yesica Paredes Rojas, Nora Mattion
H3N8 influenza virus strains have been associated with infectious disease in equine populations throughout the world. Although current vaccines for equine influenza stimulate a protective humoral immune response against the surface glycoproteins, disease in vaccinated horses has been frequently reported, probably due to poor induction of cross-reactive antibodies against non-matching strains. This work describes the performance of a recombinant protein vaccine expressed in prokaryotic cells (ΔHAp) and of a genetic vaccine (ΔHAe), both based on the conserved stem region of influenza hemagglutinin (HA) derived from A/equine/Argentina/1/93 (H3N8) virus...
March 14, 2018: Vaccine
https://www.readbyqxmd.com/read/29439436/chondrogenic-differentiation-of-defined-equine-mesenchymal-stem-cells-derived-from-umbilical-cord-blood-for-use-in-cartilage-repair-therapy
#19
Mélanie Desancé, Romain Contentin, Lélia Bertoni, Tangni Gomez-Leduc, Thomas Branly, Sandrine Jacquet, Jean-Marc Betsch, Agnès Batho, Florence Legendre, Fabrice Audigié, Philippe Galéra, Magali Demoor
Cartilage engineering is a new strategy for the treatment of cartilage damage due to osteoarthritis or trauma in humans. Racehorses are exposed to the same type of cartilage damage and the anatomical, cellular, and biochemical properties of their cartilage are comparable to those of human cartilage, making the horse an excellent model for the development of cartilage engineering. Human mesenchymal stem cells (MSCs) differentiated into chondrocytes with chondrogenic factors in a biomaterial appears to be a promising therapeutic approach for direct implantation and cartilage repair...
February 10, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29421480/advanced-nutritional-and-stem-cells-approaches-to-prevent-equine-metabolic-syndrome
#20
REVIEW
Krzysztof Marycz, Izabela Michalak, Katarzyna Kornicka
Horses metabolic disorders have become an important problem of modern veterinary medicine. Pathological obesity, insulin resistance and predisposition toward laminitis are associated with Equine Metabolic Syndrome (EMS). Based on pathogenesis of EMS, dietary and cell therapy management may significantly reduce development of this disorder. Special attention has been paid to the diet supplementation with highly bioavailable minerals and mesenchymal stem cells (MSC) which increase insulin sensitivity. In nutrition, there is a great interests in natural algae enriched via biosorption process with micro- and macroelements...
January 31, 2018: Research in Veterinary Science
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