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https://www.readbyqxmd.com/read/28702884/decellularization-of-large-tendon-specimens-combination-of-manually-performed-freeze-thaw-cycles-and-detergent-treatment
#1
Susanne Pauline Roth, Ina Erbe, Janina Burk
Reliable decellularization techniques applicable to tendon tissue play a critical role in the field of current tissue engineering. Particularly, an application as three-dimensional culture model for in vitro research and translational approaches to establish graft-based tendon repair as a routine clinical tool represent two main application fields for decellularized tendon scaffolds. Considering methodological issues of tendon decellularization, one of the major challenges lies in the preservation of the tendon-specific extracellular matrix (ECM) architecture to reflect natural tissue characteristic as best as possible...
July 13, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28697880/comparison-of-autologous-bone-marrow-and-adipose-tissue-derived-mesenchymal-stem-cells-and-platelet-rich-plasma-for-treating-surgically-induced-lesions-of-the-equine-superficial-digital-flexor-tendon
#2
A Romero, L Barrachina, B Ranera, A R Remacha, B Moreno, I de Blas, A Sanz, F J Vázquez, A Vitoria, C Junquera, P Zaragoza, C Rodellar
Several therapies have been investigated for equine tendinopathies, but satisfactory long term results have not been achieved consistently and a better understanding of the healing mechanism elicited by regenerative therapies is needed. The aim of this study was to assess the separate effects of autologous bone marrow (BM) and adipose tissue (AT) derived mesenchymal stem cells (MSCs), and platelet rich plasma (PRP), for treating lesions induced in the superficial digital flexor tendon (SDFT) of horses. Lesions were created surgically in both SDFTs of the forelimbs of 12 horses and were treated with BM-MSCs (six tendons), AT-MSCs (six tendons) or PRP (six tendons)...
June 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28681515/accuracy-of-open-magnetic-resonance-imaging-for-guiding-injection-of-the-equine-deep-digital-flexor-tendon-within-the-hoof
#3
Lauren M Groom, Nathaniel A White, M Norris Adams, Jennifer G Barrett
Lesions of the distal deep digital flexor tendon (DDFT) are frequently diagnosed using MRI in horses with foot pain. Intralesional injection of biologic therapeutics shows promise in tendon healing; however, accurate injection of distal deep digital flexor tendon lesions within the hoof is difficult. The aim of this experimental study was to evaluate accuracy of a technique for injection of the deep digital flexor tendon within the hoof using MRI-guidance, which could be performed in standing patients. We hypothesized that injection of the distal deep digital flexor tendon within the hoof could be accurately guided using open low-field MRI to target either the lateral or medial lobe at a specific location...
July 6, 2017: Veterinary Radiology & Ultrasound
https://www.readbyqxmd.com/read/28650233/evaluation-of-clinical-and-tenoscopic-findings-in-the-carpal-flexor-sheath-of-horses
#4
Sandra M Zetterström, Bengt C Johansson, James L Carmalt
OBJECTIVE To evaluate clinical and tenoscopic findings in a large group of horses undergoing surgery of the carpal flexor sheath (CFS) and determine whether any of the presurgical clinical signs were associated with tenoscopic findings. ANIMALS 242 horses that had undergone diagnostic and therapeutic tenoscopy of the CFS because of aseptic tenosynovitis. PROCEDURES Medical and tenoscopic video records (when available) of 242 horses undergoing tenoscopy of the CFS at a single equine clinic between January 2005 and June 2014 were reviewed...
July 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28583184/effect-of-single-intralesional-treatment-of-surgically-induced-equine-superficial-digital-flexor-tendon-core-lesions-with-adipose-derived-mesenchymal-stromal-cells-a-controlled-experimental-trial
#5
Florian Geburek, Florian Roggel, Hans T M van Schie, Andreas Beineke, Roberto Estrada, Kathrin Weber, Maren Hellige, Karl Rohn, Michael Jagodzinski, Bastian Welke, Christof Hurschler, Sabine Conrad, Thomas Skutella, Chris van de Lest, René van Weeren, Peter M Stadler
BACKGROUND: Adipose tissue is a promising source of mesenchymal stromal cells (MSCs) for the treatment of tendon disease. The goal of this study was to assess the effect of a single intralesional implantation of adipose tissue-derived mesenchymal stromal cells (AT-MSCs) on artificial lesions in equine superficial digital flexor tendons (SDFTs). METHODS: During this randomized, controlled, blinded experimental study, either autologous cultured AT-MSCs suspended in autologous inactivated serum (AT-MSC-serum) or autologous inactivated serum (serum) were injected intralesionally 2 weeks after surgical creation of centrally located SDFT lesions in both forelimbs of nine horses...
June 5, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28547889/comparison-between-chaotropic-and-detergent-based-sample-preparation-workflow-in-tendon-for-mass-spectrometry-analysis
#6
Yalda Ashraf Kharaz, Danae Zamboulis, Karen Sanders, Eithne Comerford, Peter Clegg, Mandy Peffers
Exploring the tendon proteome is a challenging but important task for understanding the mechanisms of physiological/pathological processes during ageing and disease and for the development of new treatments. Several extraction methods have been utilised for tendon mass spectrometry, however different extraction methods have not been simultaneously compared. In the present study we compared protein extraction in tendon with two chaotropic agents, guanidine hydrochloride (GnHCl) and urea, a detergent, RapiGest™, and their combinations for shotgun mass spectrometry...
May 26, 2017: Proteomics
https://www.readbyqxmd.com/read/28444753/examination-of-toxicity-and-collagen-linearity-after-the-administration-of-the-protein-cross-linker-genipin-in-equine-tendon-and-dermis-a-pilot-study
#7
M Bellefeuille, D F Peters, M Nolin, P Slusarewicz, D Telgenhoff
OBJECTIVE: Collagen cross-linking is an attractive therapeutic route aimed at supplementing natural collagen stabilisation. In this study the toxicity of the cross-linker genipin (GP) was examined in avascular (tendon) and vascular (dermis) tissue. METHODS: High doses of GP were injected intratendinously into three yearling horses and evaluated at various time points up to 30 days. A second group of three yearlings were injected into the dermis and evaluated at various time points up to 1 year...
May 2017: Australian Veterinary Journal
https://www.readbyqxmd.com/read/28415482/terminal-sterilization-of-equine-derived-decellularized-tendons-for-clinical-use
#8
Alessandro F Pellegata, Marta Bottagisio, Federica Boschetti, Marco Ferroni, Monica Bortolin, Lorenzo Drago, Arianna B Lovati
In the last few years, the demand for tissue substitutes has increased and decellularized matrices has been widely proposed in the medical field to restore severe damages thanks to high biocompatibility and biomechanical properties similar to the native tissues. However, biological grafts represent a potential source of contamination and disease transmission; thus, there is the need to achieve acceptable levels of sterility. Several sterilization methods have been investigated with no consensus on the outcomes in terms of minimizing structural damages and preserving functional features of the decellularized matrix for transplantation in humans...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28344975/equine-dental-pulp-connective-tissue-particles-reduced-lameness-in-horses-in-a-controlled-clinical-trial
#9
Alicia L Bertone, Nathalie A Reisbig, Allison H Kilborne, Mari Kaido, Navid Salmanzadeh, Rebecca Lovasz, Joy L Sizemore, Logan Scheuermann, Rosalind J Kopp, Lisa J Zekas, Matthew T Brokken
OBJECTIVE: To assess if injection of allogeneic dental pulp tissue particles would improve lameness in horses with naturally occurring osteoarthritis (OA) or soft tissue (ST) injury. DESIGN: Prospective, randomized, blinded, and controlled clinical trial and client survey assessment. ANIMALS: Forty lame client-owned horses. PROCEDURES: Sterile dental pulp, recovered from otherwise healthy foals that perish during dystocia, was processed under good manufacturing processing to produce mechanically manipulated, unexpanded pulp tissue particles containing viable cells surrounded in extracellular matrix...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28193263/automated-freeze-thaw-cycles-for-decellularization-of-tendon-tissue-a-pilot-study
#10
Susanne Pauline Roth, Sina Marie Glauche, Amelie Plenge, Ina Erbe, Sandra Heller, Janina Burk
BACKGROUND: Decellularization of tendon tissue plays a pivotal role in current tissue engineering approaches for in vitro research as well as for translation of graft-based tendon restoration into clinics. Automation of essential decellularization steps like freeze-thawing is crucial for the development of more standardized decellularization protocols and commercial graft production under good manufacturing practice (GMP) conditions in the future. METHODS: In this study, a liquid nitrogen-based controlled rate freezer was utilized for automation of repeated freeze-thawing for decellularization of equine superficial digital flexor tendons...
February 14, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28127617/an-anatomical-and-histological-study-of-the-equine-proximal-manica-flexoria
#11
Judith A Findley, Emanuele E Ricci, Ellen E Singer
OBJECTIVES: The main aim was to describe the gross and histological appearance of the equine manica flexoria and to identify any differences between the forelimbs and hindlimbs. An additional aim was to relate the findings to diagnostic and surgical anatomy of the manica flexoria. METHODS: Measurements of the manica flexoria were made on cadaveric limbs from horses free from pathology within the digital flexor tendon sheath. Histological sections, stained with haematoxylin and eosin and alcian- periodic acid schiff, were evaluated based on three micro-anatomical zones from dorsal to palmar or plantar...
January 27, 2017: Veterinary and Comparative Orthopaedics and Traumatology: V.C.O.T
https://www.readbyqxmd.com/read/27899062/scleraxis-is-essential-for-tendon-differentiation-by-equine-embryonic-stem-cells-and-in-equine-fetal-tenocytes
#12
Emma P Bavin, Francesca Atkinson, Tom Barsby, Debbie J Guest
The transcription factor scleraxis is required for tendon development and is upregulated during embryonic stem cell (ESC) differentiation into tenocytes. However, its role beyond early embryonic development is not defined. We utilized a short hairpin RNA to knock down scleraxis expression in ESCs and adult and fetal tenocytes. No effect on growth or morphology was observed in two-dimensional cultures. However, scleraxis knockdown in fetal tenocytes significantly reduced COL1A1, COMP, and SOX9 gene expression...
March 15, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/27898852/horse-species-symposium-biomechanics-of-the-exercising-horse
#13
Hilary M Clayton
In spite of having large height and body mass, horses are cursorial animals with an extensive gait repertoire and considerable athletic abilities. The limbs have evolved so that the heavy musculature is confined to the proximal limbs while the distal limbs are light in weight with a single functional digit and long, lightweight tendons to move and support the distal joints. These adaptations reduce the moment of inertia and decrease the energy expended to protract and retract the limbs during locomotion. There is a division of labor between the forelimbs, which have a pillar-like construction specialized for weight bearing, and the hind limbs, in which the more angulated joints provide leverage for the generation of propulsion...
October 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27881695/application-of-sonoelastography-for-evaluating-the-stiffness-of-equine-superficial-digital-flexor-tendon-during-healing
#14
N Tamura, T Kuroda, Y Kotoyori, K Fukuda, T Nukada, T Kato, A Kuwano, Y Kasashima
Sonoelastography can assess the inner stiffness of tissues. Sonoelastographic evaluation of injured equine superficial digital flexor tendons (SDFTs) is considered to be useful for assessing the stiffness of a lesion even during late-stage rehabilitation. The purpose of this study was to investigate and compare the sonoelastographic appearance of injured SDFTs over time from the onset of the injury. Eighteen horses were classified into three groups according to the length of time from injury onset: group A, within two weeks after injury; group B, approximately five months after injury; and group C, approximately nine months after injury...
February 4, 2017: Veterinary Record
https://www.readbyqxmd.com/read/27834918/identification-of-novel-equine-equus-caballus-tendon-markers-using-rna-sequencing
#15
Jan M Kuemmerle, Felix Theiss, Michal J Okoniewski, Fabienne A Weber, Sonja Hemmi, Ali Mirsaidi, Peter J Richards, Paolo Cinelli
Although several tendon-selective genes exist, they are also expressed in other musculoskeletal tissues. As cell and tissue engineering is reliant on specific molecular markers to discriminate between cell types, tendon-specific genes need to be identified. In order to accomplish this, we have used RNA sequencing (RNA-seq) to compare gene expression between tendon, bone, cartilage and ligament from horses. We identified several tendon-selective gene markers, and established eyes absent homolog 2 (EYA2) and a G-protein regulated inducer of neurite outgrowth 3 (GPRIN3) as specific tendon markers using RT-qPCR...
November 10, 2016: Genes
https://www.readbyqxmd.com/read/27810203/magic-angle-effect-on-low-field-magnetic-resonance-images-in-the-superficial-digital-flexor-tendon-in-the-equine-proximal-pastern-region
#16
C E Sherlock, T S Mair
Recognition of artefacts is an essential component of the accurate interpretation of diagnostic images. This study aimed to investigate the presence of magic angle effect in the superficial digital flexor tendon (SDFT) in the equine proximal pastern region. The proximal pastern of four cadaver limbs was imaged using a 0.27 Tesla magnet with high-resolution sequences that are commonly utilised in clinical equine practice. The limbs were imaged in a neutral position and positions that simulated the horse 'leaning in', 'leaning out' and having internal and external rotation of the distal limb...
November 2016: Veterinary Journal
https://www.readbyqxmd.com/read/27793082/dwarfism-with-joint-laxity-in-friesian-horses-is-associated-with-a-splice-site-mutation-in-b4galt7
#17
Peter A Leegwater, Manon Vos-Loohuis, Bart J Ducro, Iris J Boegheim, Frank G van Steenbeek, Isaac J Nijman, Glen R Monroe, John W M Bastiaansen, Bert W Dibbits, Leanne H van de Goor, Ids Hellinga, Willem Back, Anouk Schurink
BACKGROUND: Inbreeding and population bottlenecks in the ancestry of Friesian horses has led to health issues such as dwarfism. The limbs of dwarfs are short and the ribs are protruding inwards at the costochondral junction, while the head and back appear normal. A striking feature of the condition is the flexor tendon laxity that leads to hyperextension of the fetlock joints. The growth plates of dwarfs display disorganized and thickened chondrocyte columns. The aim of this study was to identify the gene defect that causes the recessively inherited trait in Friesian horses to understand the disease process at the molecular level...
October 28, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27761717/filarial-infection-caused-by-onchocerca-boehmi-supperer-1953-in-a-horse-from-italy
#18
Riccardo Paolo Lia, Yasen Mutafchiev, Vincenzo Veneziano, Alessio Giannelli, Francesca Abramo, Mario Santoro, Maria Stefania Latrofa, Cinzia Cantacessi, Coralie Martin, Domenico Otranto, Andrea Bertuglia, Barbara Riccio
Equids can be infected by a range of skin-dwelling filarial nematodes, including four species of the genus Onchocerca. Current literature on equine onchocercosis is fragmentary and often limited to isolated case reports. The present study aimed to describe a clinical case of equine onchocercosis caused by Onchocerca boehmi (Supperer, 1953) (syn. Elaeophora boehmi) in an 8-year-old gelding Belgian show jumper from northern Italy. The horse was presented with a firm and painless mass on the proximal third of the right metacarpal region...
January 2017: Parasitology Research
https://www.readbyqxmd.com/read/27746821/mri-based-assessment-of-intralesional-delivery-of-bone-marrow-derived-mesenchymal-stem-cells-in-a-model-of-equine-tendonitis
#19
Alexandra Scharf, Shannon P Holmes, Merrilee Thoresen, Jennifer Mumaw, Alaina Stumpf, John Peroni
Ultrasound-guided intralesional injection of mesenchymal stem cells (MSCs) is held as the benchmark for cell delivery in tendonitis. The primary objective of this study was to investigate the immediate cell distribution following intralesional injection of MSCs. Unilateral superficial digital flexor tendon (SDFT) lesions were created in the forelimb of six horses and injected with 10 × 10(6) MSCs labeled with superparamagnetic iron oxide nanoparticles (SPIOs) under ultrasound guidance. Assays were performed to confirm that there were no significant changes in cell viability, proliferation, migration, or trilineage differentiation due to the presence of SPIOs...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27726447/exposure-of-a-tendon-extracellular-matrix-to-synovial-fluid-triggers-endogenous-and-engrafted-cell-death-a-mechanism-for-failed-healing-of-intrathecal-tendon-injuries
#20
Elaine R Garvican, Mazdak Salavati, Roger K W Smith, Jayesh Dudhia
AIM: The purpose of this study was to investigate the effect of normal synovial fluid (SF) on exposed endogenous tendon-derived cells (TDCs) and engrafted mesenchymal stem cells (MSCs) within the tendon extracellular matrix. METHODS: Explants from equine superficial digital flexor (extra-synovial) and deep digital flexor tendons (DDFTs) from the compressed, intra-synovial and the tensile, extra-synovial regions were cultured in allogeneic or autologous SF-media...
October 11, 2016: Connective Tissue Research
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