Alireza Shiri, Jamal Sarvari, Saeed Firoozi Ghahestani, Nasser Gholijani, Ali Mohammad Tamaddon, Mahroo Rastegari, Afagh Moattari, Seyed Younes Hosseini
The role of coagulation factors on the inflammatory effect of adenovirus (Ad) is an unresolved question that was considered herein. Adenovirus-36(Ad36) and adenovector-5-GFP(Ad5-GFP) were prepared; then, they were loaded with VII or FX factors. The size/charge parameters and transduction efficiency were evaluated using fluorescent microscopy and Zetasizer, respectively. The Ad36-coagulation factor complexes were added on the stellate cells, LX-2. Thereafter, the expression levels of inflammatory and fibrotic genes including PKR, IL-1β, TNF-α, TIMP-1, collagen, and TGF-β were measured by qPCR and ELISA assays...
January 7, 2021: Current Microbiology