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Cyclin A1

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https://www.readbyqxmd.com/read/29207139/cpne1-silencing-inhibits-the-proliferation-invasion-and-migration-of-human-osteosarcoma-cells
#1
Zhenhuan Jiang, Jiannong Jiang, Bizeng Zhao, Huilin Yang, Yunliang Wang, Shang Guo, Youping Deng, Deyi Lu, Tieliang Ma, Hongwei Wang, Jinzhi Wang
Osteosarcoma (OS) is the most common primary malignancy of the bone affecting children and adolescents. Copine 1 (CPNE1) is a highly conserved calcium-dependent phospholipid-binding protein and may function in regulating signal transduction and membrane trafficking. In the present study, we investigated CPNE1 expression in osteosarcoma tissues and cells, and studied the effects of small interfering RNA (siRNA)-targeting CPNE1 on proliferation, metastasis and chemosensitivity of the osteosarcoma cells. The results demonstrated that CPNE1 was highly expressed in the osteosarcoma tissues and cell lines...
December 4, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28985758/pharmacological-targeting-of-the-ephrin-receptor-kinase-signalling-by-glpg1790-in-vitro-and-in-vivo-reverts-oncophenotype-induces-myogenic-differentiation-and-radiosensitizes-embryonal-rhabdomyosarcoma-cells
#2
Francesca Megiorni, Giovanni Luca Gravina, Simona Camero, Simona Ceccarelli, Andrea Del Fattore, Vincenzo Desiderio, Federica Papaccio, Heather P McDowell, Rajeev Shukla, Antonio Pizzuti, Filip Beirinckx, Philippe Pujuguet, Laurent Saniere, Ellen Van der Aar, Roberto Maggio, Francesca De Felice, Cinzia Marchese, Carlo Dominici, Vincenzo Tombolini, Claudio Festuccia, Francesco Marampon
BACKGROUND: EPH (erythropoietin-producing hepatocellular) receptors are clinically relevant targets in several malignancies. This report describes the effects of GLPG1790, a new potent pan-EPH inhibitor, in human embryonal rhabdomyosarcoma (ERMS) cell lines. METHODS: EPH-A2 and Ephrin-A1 mRNA expression was quantified by real-time PCR in 14 ERMS tumour samples and in normal skeletal muscle (NSM). GLPG1790 effects were tested in RD and TE671 cell lines, two in vitro models of ERMS, by performing flow cytometry analysis, Western blotting and immunofluorescence experiments...
October 6, 2017: Journal of Hematology & Oncology
https://www.readbyqxmd.com/read/28948561/relationship-between-the-contents-of-cyclins-cyclin-dependent-kinases-and-their-inhibitors-in-whole-blood-mononuclear-leukocytes-during-the-postclinical-stage-of-community-acquired-pneumonia-under-the-influence-of-1-ghz-microwaves
#3
V G Zilov, A A Khadartsev, I V Terekhov, S S Bondar'
The contents of cyclins A1, B1, E1, and D1, cyclin-dependent kinases 2 and 4 (CDK2 and CDK4), cyclin-dependent kinase 2C inhibitor (p18), and cyclin-dependent kinase 1C inhibitor (p57) in whole blood mononuclear cells of practically healthy donors and convalescents from community-acquired pneumonia were measured after cell exposure to low-intensity electromagnetic radiation of 1 GHz. The level of cyclins A1, D1, and B1, p57, and p18 proteins, and CDK2 was reduced in convalescents, while the content of cyclin B1 was elevated in both groups...
September 26, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28870911/sunitinib-inhibits-breast-cancer-cell-proliferation-by-inducing-apoptosis-cell-cycle-arrest-and-dna-repair-while-inhibiting-nf-%C3%AE%C2%BAb-signaling-pathways
#4
Hesham M Korashy, Zaid H Maayah, Fawaz E Al Anazi, Abdulaziz M Alsaad, Ibrahim O Alanazi, Osamah M Belali, Fahad O Al-Atawi, Aws Alshamsan
The tyrosine kinase inhibitor sunitinib was recently approved for use against gastrointestinal stromal tumors and advanced renal cell carcinoma. Yet, the protective effect of sunitinib against breast cancer has been poorly investigated. In this study, we investigated the antiproliferative and apoptogenic effects of sunitinib and the possible mechanism involved against the MCF7 human breast cancer cell line. Treatment of MCF7 cells with sunitinib caused concentration-dependent cell growth suppression due to apoptosis...
September 2017: Anticancer Research
https://www.readbyqxmd.com/read/28803881/cell-specific-and-dose-dependent-effects-of-pahs-on-proliferation-cell-cycle-and-apoptosis-protein-expression-and-hormone-secretion-by-placental-cell-lines
#5
Eliza Drwal, Agnieszka Rak, Adam Grochowalski, Tomasz Milewicz, Ewa Lucja Gregoraszczuk
In the preset study we measured the concentrations of 16 priority PAHs in maternal blood and placental tissue by using the GC-MS/MS system, and investigated the effects of selected PAHs (naphthalene, anthracene, phenanthrene, pyrene) and mixtures on BeWo and JEG-3 human placental cell line proliferation (Alamar Blue), cytotoxicity (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (XTT), lactate dehydrogenase (LDH), acid phosphatase (AP), endocrine activity (progesterone and estradiol secretion) and apoptosis (cyclin A1, cyclin D2, cdk 2, cdk 4, Bcl-xl, Bax, and caspase-3 protein expression)...
October 5, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28777434/bifunctional-role-of-ephrin-a1-eph-system-in-stimulating-cell-proliferation-and-protecting-cells-from-cell-death-through-the-attenuation-of-er-stress-and-inflammatory-responses-in-bovine-mammary-epithelial-cells
#6
Minkyung Kang, Wooyoung Jeong, Hyocheol Bae, Whasun Lim, Fuller W Bazer, Gwonhwa Song
Structural and functional development of the mammary gland is constant in the mammary gland life cycle. Eph receptors and their ligands, ephrins, control events through cell-to-cell interactions during embryonic development and adult tissue homeostasis; however, little information on participation of ephrin A1, a representative ligand of the Eph receptor, in the development and function of normal mammary glands is known. In this study, we demonstrated functional effects of the ephrin A1-Eph system and mechanisms of its action on bovine mammary epithelial (MAC-T) cells...
August 4, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28737823/overexpression-of-non%C3%A2-smc-condensin%C3%A2-i-complex-subunit%C3%A2-g-serves-as-a-promising-prognostic-marker-and-therapeutic-target-for-hepatocellular-carcinoma
#7
Wanwei Liu, Bo Liang, Hongliang Liu, Yong Huang, Xiangbao Yin, Fan Zhou, Xin Yu, Qian Feng, Enliang Li, Zhenhong Zou, Linquan Wu
The non‑SMC condensin I complex subunit G (NCAPG) that organizes the coiling topology of individual chromatids, represents an overexpressed antigen in various types of cancer, and also contributes to restructuring chromatin into rod‑shaped mitotic chromosomes and ensuring the segregation of sister chromatid during cell division. In this study, we investigated the association between NCAPG expression and the biological behavior of hepatocellular carcinoma (HCC) to further explore the potential of NCAPG as a therapeutic target...
September 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28670110/cryptotanshinone-induces-cell-cycle-arrest-and-apoptosis-through-the-jak2-stat3-and-pi3k-akt-nf%C3%AE%C2%BAb-pathways-in-cholangiocarcinoma-cells
#8
Fayong Ke, Zheng Wang, Xiaoling Song, Qiang Ma, Yunping Hu, Lin Jiang, Yijian Zhang, Yingbin Liu, Yong Zhang, Wei Gong
BACKGROUND: Cholangiocarcinoma (CCA) is the most common biliary tract malignancy in the world with high resistance to current chemotherapies and extremely poor prognosis. The main objective of this study was to investigate the inhibitory effects of cryptotanshinone (CTS), a natural compound isolated from Salvia miltiorrhiza Bunge, on CCA both in vitro and in vivo and to explore the underlying mechanisms of CTS-induced apoptosis and cell cycle arrest. METHODS: The anti-tumor activity of CTS on HCCC-9810 and RBE cells was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay and colony forming assays...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28627629/reducing-autophagy-and-inducing-g1-phase-arrest-by-aloperine-enhances-radio-sensitivity-in-lung-cancer-cells
#9
Zhijie Xu, Yuanliang Yan, Shuangshuang Zeng, Long Qian, Shuang Dai, Lingfang Xiao, Lin Wang, Xue Yang, Yi Xiao, Zhicheng Gong
Aloperine (ALO), an isolated alkaloid from the leaves of Sophora alopecuroides (S. alopecuroides), has been suggested to exhibit anti-inflammatory and antitumor properties, and has been traditionally used to treat various diseases, including cancers. However, little is known about the effects of ALO on the radio-sensitivity of lung cancer cells. In the present study, we confirmed that agent ALO inhibits cell growth, promotes cell aopotosis and induces G1 phase arrest and consequently enhanced the radio-sensitivity in radio-resistant lung cancer cells A549/IR...
June 19, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28608720/diagnostic-algorithm-of-common-mature-b-cell-lymphomas-by-immunohistochemistry
#10
REVIEW
Huan-You Wang, Youli Zu
CONTEXT: - Different types of mature B-cell lymphomas, including plasma cell neoplasms, exhibit distinct immunohistochemical profiles, which enable them to be correctly diagnosed. However, except for rare examples of lymphoma-specific immunohistochemistry, such as cyclin D1 in mantle cell lymphoma and annexin A1 in hairy cell leukemia, immunohistochemical profiles of mature B-cell lymphomas overlap and lack specificity. OBJECTIVES: - To systemically review immunohistochemical features associated with commonly encountered mature B-cell lymphomas based on the presence or absence of CD5 and CD10; to review the immunophenotypic profile of plasma cells derived from plasma cell myelomas and B-cell lymphomas; and to review a group of rare, aggressive B-cell lymphomas with antigen expression features of plasma cells...
September 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/28569402/lrwd1-regulates-microtubule-nucleation-and-proper-cell-cycle-progression-in-the-human-testicular-embryonic-carcinoma-cells
#11
Chia-Yih Wang, Yu-Han Hong, Jhih-Siang Syu, Yung-Chieh Tsai, Xiu-Ying Liu, Ting-Yu Chen, Yin-Mei Su, Pao-Lin Kuo, Yung-Ming Lin, Yen-Ni Teng
Leucine-rich repeats and WD repeat domain containing protein 1 (LRWD1) is a testis-specific protein that mainly expressed in the sperm neck where centrosome is located. By using microarray analysis, LRWD1 is identified as a putative gene that involved in spermatogenesis. However, its role in human male germ cell development has not been extensively studied. When checking in the semen of patients with asthenozoospermia, teratozoospermia, and asthenoteratozoospermia, the level of LRWD1 in the sperm neck was significantly reduced with a defective neck or tail...
June 1, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28555054/knockdown-of-xbp1-by-rnai-in-mouse-granulosa-cells-promotes-apoptosis-inhibits-cell-cycle-and-decreases-estradiol-synthesis
#12
Nan Wang, Fan Zhao, Pengfei Lin, Guangle Zhang, Keqiong Tang, Aihua Wang, Yaping Jin
Granulosa cells are crucial for follicular growth, development, and follicular atresia. X-box binding protein 1 (XBP1), a basic region-leucine zipper protein, is widely involved in cell differentiation, proliferation, apoptosis, cellular stress response, and other signaling pathways. In this study, RNA interference, flow cytometry, western blot, real-time PCR, Cell Counting Kit (CCK8), and ELISA were used to investigate the effect of XBP1 on steroidogenesis, apoptosis, cell cycle, and proliferation of mouse granulosa cells...
May 29, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28521488/mir-137-inhibits-the-proliferation-of-human-non-small-cell-lung-cancer-cells-by-targeting-src3
#13
Ruilin Chen, Yongqing Zhang, Chengcheng Zhang, Hua Wu, Shumei Yang
Non-small cell lung cancer (NSCLC) is the most common type of lung cancer. The results of the present study demonstrate that high expression of microRNA (miR)-137 and low expression of steroid receptor coactivator-3 (SRC3) had a significant negative correlation in 40 NSCLC tissue samples. In addition, cell colony formation and proliferation was significantly reduced in miR-137-transfected A549 and NCI-H838 cells compared with scramble-transfected NSCLC cell lines. miR-137 was identified to induce G1/S cell cycle arrest and dysregulate the mRNA expression of cell cycle-associated proteins (proliferating cell nuclear antigen, cyclin E, cyclin A1, cyclin A2 and p21) in NSCLC cells...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28488543/the-transcription-factor-foxa1-induces-epithelial-ovarian-cancer-tumorigenesis-and-progression
#14
Li-Li Wang, Yin-Ling Xiu, Xi Chen, Kai-Xuan Sun, Shuo Chen, Dan-Dan Wu, Bo-Liang Liu, Yang Zhao
FOXA1 (forkhead box A1), a member of the FOXA transcription factor superfamily, plays an important role in tumor occurrence and development. However, the relationship between FOXA1 and ovarian cancer has not been reported. We examined normal ovarian tissue and ovarian cancer tissue and found increased FOXA1 expression in the cancer tissue. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry assays demonstrated that transfection with small interfering RNA to silence FOXA1 (si-FOXA1) in ovarian cancer cell lines decreased cell proliferation and induced apoptosis and S-phase arrest...
May 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28414743/the-et-1-mediated-carbonylation-and-degradation-of-anxa1-induce-inflammatory-phenotype-and-proliferation-of-pulmonary-artery-smooth-muscle-cells-in-hps
#15
Jing He, Bin Yi, Yang Chen, Qing Huang, Huan Wang, Kaizhi Lu, Weiling Fu
Hepatopulmonary syndrome (HPS) is a serious complication of advanced liver disease, which markedly increases mortality. Pulmonary vascular remodelling (PVR) induced by circulating mediators plays an important role in the pathogenesis of HPS, while the underlying mechanism remains undefined. In the present study, we reported that endothelin-1 (ET-1) is up-regulated and annexin A1(ANXA1) is down-regulated in HPS rat, and ET-1 decreases the ANXA1 expression in a dose-dependent manner in rat pulmonary arterial smooth muscle cells (PASMCs)...
2017: PloS One
https://www.readbyqxmd.com/read/28343894/-1-9-n%C3%AE-c-crourorb-a1-isolated-from-croton-urucurana-latex-induces-g2-m-cell-cycle-arrest-and-apoptosis-in-human-hepatocarcinoma-cells
#16
Priscila de Matos Cândido-Bacani, Frédéric Ezan, Patrícia de Oliveira Figueiredo, Maria de Fátima Cepa Matos, Fernanda Rodrigues Garcez, Walmir Silva Garcez, Georges Baffet
[1-9-NαC]-crourorb A1 is a cyclic peptide isolated from Croton urucurana Baillon latex, found in midwestern Brazil, that has been shown to exert cytotoxic effects against a panel of cancer cell lines. However, the underlying mechanisms responsible for the crourorb A1-induced cytotoxicity in cancer cells remain unknown. In this study, the effects of crourorb A1 on the viability, apoptosis, cell cycle and migration of Huh-7 (human hepatocarcinoma) cells were investigated. We evaluated the viability of Huh-7 cells treated with crourorb A1 in 2D and 3D collagen cultures and found that cells in 3D culture exhibited increased resistance to crourorb A1 compared to cells in 2D culture (IC50: 62μg/ml versus 35...
May 5, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28301569/meiotic-failure-in-cyclin-a1-deficient-mouse-spermatocytes-triggers-apoptosis-through-intrinsic-and-extrinsic-signaling-pathways-and-14-3-3-proteins
#17
Sunil K Panigrahi, Marcia Manterola, Debra J Wolgemuth
Cyclin A1 (Ccna1), a member of the mammalian A type cyclins, is most abundantly expressed in spermatocytes and is essential for spermatogenesis in the mouse. Ccna1- deficient spermatocytes arrest at late meiotic prophase and undergo apoptosis. To further delineate the mechanisms and key factors involved in this process, we have examined changes in expression of genes involved in both intrinsic and extrinsic signaling pathways that trigger apoptosis in the mutant spermatocytes. Our results show that both pathways are involved, and that the factors involved in the intrinsic pathway were expressed earlier than those involved in the extrinsic pathway...
2017: PloS One
https://www.readbyqxmd.com/read/28214847/contribution-of-p62-to-phenotype-transition-of-coronary-arterial-myocytes-with-defective-autophagy
#18
Junxiang Bao, Guangbi Li, Xinxu Yuan, Pin-Lan Li, Erich Gulbins
BACKGROUND: Autophagy disorder contributes to dedifferentiation of arterial smooth muscle cells, but the mechanisms are poorly understood. Here, we sought to investigate the role of scaffolding adaptor p62/SQSTM1 (p62) in phenotype switching of mouse coronary arterial myocytes (CAMs) induced by CD38 gene deficiency or lysosomal dysfunction which blocks autophagic flux in the cells. METHODS: Protein expression was measured by western blot analysis and immunofluorescent staining...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28202053/up-regulation-of-mir-146a-increases-the-sensitivity-of-non-small-cell-lung-cancer-to-ddp-by-downregulating-cyclin-j
#19
Lin Shi, Zhaozhong Xu, Gang Wu, Xiaoting Chen, Yuanyuan Huang, Yanjing Wang, Weiqiang Jiang, Bin Ke
BACKGROUND: Cisplatin (DDP)-based chemotherapy is the common first-line therapy for lung cancer. However, their efficacy is often limited by primary drug resistance and/or acquired drug resistance. The aim of this study was to investigate the function of miRNA-146a (miR-146a) in DDP-resistant non-small cell lung cancer (NSCLC), as well as the underlying mechanisms. METHODS: The effect of overexpression of miR-146a and/or knockdown of cyclin J (CCNJ) in A549/DDP and SPC-A1/DDP cells were investigated as follows...
February 15, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28115626/prmt5-regulates-ires-dependent-translation-via-methylation-of-hnrnp-a1
#20
Guozhen Gao, Surbhi Dhar, Mark T Bedford
The type II arginine methyltransferase PRMT5 is responsible for the symmetric dimethylation of histone to generate the H3R8me2s and H4R3me2s marks, which correlate with the repression of transcription. However, the protein level of a number of genes (MEP50, CCND1, MYC, HIF1a, MTIF and CDKN1B) are reported to be downregulated by the loss of PRMT5, while their mRNA levels remain unchanged, which is counterintuitive for PRMT5's proposed role as a transcription repressor. We noticed that the majority of the genes regulated by PRMT5, at the posttranscriptional level, express mRNA containing an internal ribosome entry site (IRES)...
May 5, 2017: Nucleic Acids Research
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