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Cyclin A1

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https://www.readbyqxmd.com/read/28301569/meiotic-failure-in-cyclin-a1-deficient-mouse-spermatocytes-triggers-apoptosis-through-intrinsic-and-extrinsic-signaling-pathways-and-14-3-3-proteins
#1
Sunil K Panigrahi, Marcia Manterola, Debra J Wolgemuth
Cyclin A1 (Ccna1), a member of the mammalian A type cyclins, is most abundantly expressed in spermatocytes and is essential for spermatogenesis in the mouse. Ccna1- deficient spermatocytes arrest at late meiotic prophase and undergo apoptosis. To further delineate the mechanisms and key factors involved in this process, we have examined changes in expression of genes involved in both intrinsic and extrinsic signaling pathways that trigger apoptosis in the mutant spermatocytes. Our results show that both pathways are involved, and that the factors involved in the intrinsic pathway were expressed earlier than those involved in the extrinsic pathway...
2017: PloS One
https://www.readbyqxmd.com/read/28214847/contribution-of-p62-to-phenotype-transition-of-coronary-arterial-myocytes-with-defective-autophagy
#2
Junxiang Bao, Guangbi Li, Xinxu Yuan, Erich Gulbins, Pin-Lan Li
BACKGROUND: Autophagy disorder contributes to dedifferentiation of arterial smooth muscle cells, but the mechanisms are poorly understood. Here, we sought to investigate the role of scaffolding adaptor p62/SQSTM1 (p62) in phenotype switching of mouse coronary arterial myocytes (CAMs) induced by CD38 gene deficiency or lysosomal dysfunction which blocks autophagic flux in the cells. METHODS: Protein expression was measured by western blot analysis and immunofluorescent staining...
February 3, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28202053/up-regulation-of-mir-146a-increases-the-sensitivity-of-non-small-cell-lung-cancer-to-ddp-by-downregulating-cyclin-j
#3
Lin Shi, Zhaozhong Xu, Gang Wu, Xiaoting Chen, Yuanyuan Huang, Yanjing Wang, Weiqiang Jiang, Bin Ke
BACKGROUND: Cisplatin (DDP)-based chemotherapy is the common first-line therapy for lung cancer. However, their efficacy is often limited by primary drug resistance and/or acquired drug resistance. The aim of this study was to investigate the function of miRNA-146a (miR-146a) in DDP-resistant non-small cell lung cancer (NSCLC), as well as the underlying mechanisms. METHODS: The effect of overexpression of miR-146a and/or knockdown of cyclin J (CCNJ) in A549/DDP and SPC-A1/DDP cells were investigated as follows...
February 15, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28115626/prmt5-regulates-ires-dependent-translation-via-methylation-of-hnrnp-a1
#4
Guozhen Gao, Surbhi Dhar, Mark T Bedford
The type II arginine methyltransferase PRMT5 is responsible for the symmetric dimethylation of histone to generate the H3R8me2s and H4R3me2s marks, which correlate with the repression of transcription. However, the protein level of a number of genes (MEP50, CCND1, MYC, HIF1a, MTIF and CDKN1B) are reported to be downregulated by the loss of PRMT5, while their mRNA levels remain unchanged, which is counterintuitive for PRMT5's proposed role as a transcription repressor. We noticed that the majority of the genes regulated by PRMT5, at the posttranscriptional level, express mRNA containing an internal ribosome entry site (IRES)...
January 23, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28110416/lasiokaurin-derivatives-synthesis-antimicrobial-and-antitumor-biological-evaluation-and-apoptosis-inducing-effects
#5
Da-Hong Li, Ping Hu, Sheng-Tao Xu, Chun-Yan Fang, Shuang Tang, Xin-Yu Wang, Xing-Yan Sun, He Li, Ying Xu, Xiao-Ke Gu, Jin-Yi Xu
Herein, a series of lasiokaurin derivatives were designed and synthesized. All the derivatives together with lasiokaurin and oridonin were tested for their antimicrobial and antiproliferative activity. Compound 16 showed the most promising antimicrobial activity with MICs of 2.0 and 1.0 μg/mL against Gram-Positive bacteria S. aureus and B. subtilis, respectively. All the synthetic lasiokaurin derivatives showed better antiproliferative activity than parent compound lasiokaurin 1. Compound 10 exhibited the strongest cytotoxicity with IC50 values of 0...
January 21, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/28076854/open-angle-glaucoma-drug-development-pipeline-during-the-last-20-years-1995-2015
#6
André Vicente, Sylvie Prud'homme, Joana Ferreira, Luís Abegão Pinto, Ingeborg Stalmans
OBJECTIVES: To analyse drug development for open-angle glaucoma during the last 20 years. METHODS: Research was performed by referring to clinical trials registered at the International Clinical Trials Registry Platform (ICTRP). A search for the condition "open-angle glaucoma" with the intervention "drug" was performed. We included trials registered from 01/01/1995 to 01/01/2015, only involving studies in phases 1, 2, and 3. Only studies resorting to novel treatment strategies (either novel drugs or yet-untested fixed associations of approved medication) were considered...
January 12, 2017: Ophthalmic Research
https://www.readbyqxmd.com/read/27984373/hnrnpa1-a-splicing-regulator-is-an-effective-target-protein-for-cervical-cancer-detection-comparison-with-conventional-tumor-markers
#7
Young-Jon Kim, Byoung-Ryun Kim, Jae-Suk Ryu, Gyeong-Ok Lee, Hak-Ryul Kim, Keum-Ha Choi, Jae-Won Ryu, Kyoung-Suk Na, Min-Cheol Park, Hong-Seob So, Ji-Hyun Cho, Do-Sim Park
OBJECTIVE: Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1), serine/arginine-rich splicing factor 1 (SRSF1), and SRSF3 are splicing regulators associated with oncogenesis. However, the alterations of SF proteins and their diagnostic values in cervical cancer are unclear. To apply SFs clinically, effective marker selection and characterization of the target organ properties are essential. MATERIALS AND METHODS: We concurrently analyzed HNRNPA1, SRSF1, SRSF3, and the conventional tumor markers squamous cell carcinoma antigen (SCCA) and carcinoembryonic antigen (CEA) in cervical tissue samples (n = 127) using semiquantitative immunoblotting...
February 2017: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/27973579/knockdown-of-creb3-luman-by-shrna-in-mouse-granulosa-cells-results-in-decreased-estradiol-and-progesterone-synthesis-and-promotes-cell-proliferation
#8
Fan Zhao, Nan Wang, Yanglei Yi, Pengfei Lin, Keqiong Tang, Aihua Wang, Yaping Jin
Luman (also known as LZIP or CREB3) is a transcription factor and a member of the cAMP responsive element-binding (CREB) family proteins. Although Luman has been detected in apoptotic granulosa cells and disorganized atretic bodies, the physiological function of Luman in follicular development has not been reported. Our objective is to determine the role of Luman in folliculogenesis by knocking down Luman expression in mouse GCs (granulosa cells) using shRNA. Luman expression was successfully knocked down in mouse GCs at the mRNA and protein level, as confirmed by real-time quantitative PCR, western blot and immunofluorescence staining, respectively...
2016: PloS One
https://www.readbyqxmd.com/read/27884605/arsenic-induced-activation-of-the-homeodomain-interacting-protein-kinase-2-hipk2-to-camp-response-element-binding-protein-creb-axis
#9
Kazunori Hashimoto, Yoshiaki Tsuji
Cyclic AMP-response element-binding protein (CREB) plays key transcriptional roles in cell metabolism, proliferation, and survival. Ser133 phosphorylation by protein kinase A (PKA) is a well-characterized CREB activation mechanism. Homeodomain-interacting protein kinase (HIPK) 2, a nuclear serine/threonine kinase, activates CREB through Ser271 phosphorylation; however, the regulatory mechanism remains uncharacterized. Transfection of CREB in HEK293 cells together with the kinase demonstrated that HIPK2 phosphorylated CREB at Ser271 but not Ser133; likewise, PKA phosphorylated CREB at Ser133 but not Ser271, suggesting two distinct CREB regulatory mechanisms by HIPK2 and PKA...
January 6, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/27880045/koelreuteria-formosana-extract-induces-growth-inhibition-and-cell-death-in-human-colon-carcinoma-cells-via-g2-m-arrest-and-lc3-ii-activation-dependent-autophagy
#10
Chi-Ting Horng, Yueh-Jung Wu, Pei-Ni Chen, Shu-Chen Chu, Chun-Miao Tsai, Yih-Shou Hsieh
Autophagy is a self-destructive process that degrades cytoplasmic constituents. In our previous study, Koelreuteria formosana ethanolic extract (KFEE), which is obtained from natural plants endemic to Taiwan, has inhibited cell metastasis in renal carcinoma cells. However, the anticancer effects of KFEE on colon cancer remain unclear. In this study, KFEE exerted a strong cytotoxic effect on DLD-1 and COLO 205 human colorectal cancer cell lines. KFEE effectively inhibited cancer cell proliferation, induced G2/M-phase arrest associated with downregulaton of cyclin E, cyclin B and cdc25C and upregulation of p21, and induced cell death by activating autophagy but did not cause apoptotic cell death...
January 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/27845389/bafilomycin-a1-induces-caspase-independent-cell-death-in-hepatocellular-carcinoma-cells-via-targeting-of-autophagy-and-mapk-pathways
#11
Yumei Yan, Ke Jiang, Peng Liu, Xianbin Zhang, Xin Dong, Jingchun Gao, Quentin Liu, Martin P Barr, Quan Zhang, Xiukun Hou, Songshu Meng, Peng Gong
Hepatocellular carcinoma (HCC) is refractory to chemotherapies, necessitating novel effective agents. The lysosome inhibitor Bafilomycin A1 (BafA1) at high concentrations displays cytotoxicity in a variety of cancers. Here we show that BafA1 at nanomolar concentrations suppresses HCC cell growth in both 2 dimensional (2D) and 3D cultures. BafA1 induced cell cycle arrest in the G1 phase and triggered Cyclin D1 turnover in HCC cells in a dual-specificity tyrosine phosphorylation-regulated kinase 1B (DYRK1B) dependent manner...
November 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27792641/lim-domain-only-2-regulates-endothelial-proliferation-angiogenesis-and-tissue-regeneration
#12
Shu Meng, Gianfranco Matrone, Jie Lv, Kaifu Chen, Wing Tak Wong, John P Cooke
BACKGROUND: LIM domain only 2 (LMO2, human gene) is a key transcription factor that regulates hematopoiesis and vascular development. However, its role in adult endothelial function has been incompletely characterized. METHODS AND RESULTS: In vitro loss- and gain-of-function studies on LMO2 were performed in human umbilical vein endothelial cells with lentiviral overexpression or short hairpin RNA knockdown (KD) of LMO2, respectively. LMO2 KD significantly impaired endothelial proliferation...
October 6, 2016: Journal of the American Heart Association
https://www.readbyqxmd.com/read/27698802/identification-of-genes-involved-in-epstein-barr-virus-associated-nasopharyngeal-carcinoma
#13
Junguo Wang, Fang Mei, Xia Gao, Shoulin Wang
Nasopharyngeal carcinoma (NPC) is the most common cancer originating from the nasopharynx, and can be induced by infection with Epstein-Barr virus (EBV). To study the mechanisms of EBV-associated NPC, a microarray of the GSE12452 dataset was analyzed. GSE12452 was downloaded from Gene Expression Omnibus and consisted of 31 NPC samples and 10 normal healthy nasopharyngeal tissue samples. The differentially-expressed genes (DEGs) were screened using the linear models for microarray data package in R. Using Database for Annotation, Visualization and Integrated Discovery software, potential functions of the DEGs were predicted by Gene Ontology and pathway enrichment analyses...
October 2016: Oncology Letters
https://www.readbyqxmd.com/read/27669502/cyclin-a1-expression-and-paclitaxel-resistance-in-human-ovarian-cancer-cells
#14
Kuan-Chun Huang, Junzheng Yang, Michelle C Ng, Shu-Kay Ng, William R Welch, Michael G Muto, Ross S Berkowitz, Shu-Wing Ng
BACKGROUND: The development of intrinsic and acquired resistance to antineoplastic agents is a major obstacle to successful chemotherapy in ovarian cancers. Identification and characterisation of chemoresponse-associated biomarkers are of paramount importance for novel therapeutic development. METHODS: Global RNA expression profiles were obtained by high-throughput microarray analysis. Cell cycle, proliferation rate, and paclitaxel sensitivity of ovarian cancer cells harbouring cyclin A1-inducible expression construct were compared with and without tetracycline induction, as well as when the cyclin A1 expression was suppressed by short inhibiting RNA (siRNA)...
November 2016: European Journal of Cancer
https://www.readbyqxmd.com/read/27569210/smyd3-mediated-h2a-z-1-methylation-promotes-cell-cycle-and-cancer-proliferation
#15
Cheng-Hui Tsai, Yun-Ju Chen, Chia-Jung Yu, Shiou-Ru Tzeng, I-Chen Wu, Wen-Hung Kuo, Ming-Chieh Lin, Nei-Li Chan, Kou-Juey Wu, Shu-Chun Teng
SMYD3 methyltransferase is nearly undetectable in normal human tissues but highly expressed in several cancers, including breast cancer, although its contributions to pathogenesis in this setting are unclear. Here we report that histone H2A.Z.1 is a substrate of SMYD3 that supports malignancy. SMYD3-mediated dimethylation of H2A.Z.1 at lysine 101 (H2A.Z.1K101me2) increased stability by preventing binding to the removal chaperone ANP32E and facilitating its interaction with histone H3. Moreover, a microarray analysis identified cyclin A1 as a target coregulated by SMYD3 and H2A...
October 15, 2016: Cancer Research
https://www.readbyqxmd.com/read/27551490/sundew-plant-a-potential-source-of-anti-inflammatory-agents-selectively-induces-g2-m-arrest-and-apoptosis-in-mcf-7-cells-through-upregulation-of-p53-and-bax-bcl-2-ratio
#16
N B Ghate, A Das, D Chaudhuri, S Panja, N Mandal
The worldwide cancer incidences are remarkable despite the advancement in cancer drug discovery field, highlighting the need for new therapies focusing on cancer cell and its microenvironment, including inflammation. Several species of Drosera (family: Droseraceae) are used in various traditional as well as homeopathic systems of medicine. Drosera burmannii Vahl. is also enlisted in French Pharmacopoeia in 1965 for the treatment of inflammatory diseases, including chronic bronchitis, asthma and whooping cough...
2016: Cell Death Discovery
https://www.readbyqxmd.com/read/27460030/endosulfan-inhibiting-the-meiosis-process-via-depressing-expressions-of-regulatory-factors-and-causing-cell-cycle-arrest-in-spermatogenic-cells
#17
Fang-Zi Guo, Lian-Shuang Zhang, Jia-Liu Wei, Li-Hua Ren, Jin Zhang, Li Jing, Man Yang, Ji Wang, Zhi-Wei Sun, Xian-Qing Zhou
Endosulfan is a persistent organic pollutant and widely used in agriculture as a pesticide. It is present in air, water, and soil worldwide; therefore, it is a health risk affecting especially the reproductive system. The aim of this study was to evaluate the toxicity of endosulfan in the reproductive system. To investigate the effect of endosulfan on meiosis process, 32 rats were divided into four groups, treated with 0, 1, 5, and 10 mg/kg/day endosulfan, respectively, and sacrificed after the 21 days of treatments...
October 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27388959/identification-of-cyclins-a1-e1-and-vimentin-as-downstream-targets-of-heme-oxygenase-1-in-vascular-endothelial-growth-factor-mediated-angiogenesis
#18
Andrea Bauer, Hayley Mylroie, C Clare Thornton, Damien Calay, Graeme M Birdsey, Allan P Kiprianos, Garrick K Wilson, Miguel P Soares, Xiaoke Yin, Manuel Mayr, Anna M Randi, Justin C Mason
Angiogenesis is an essential physiological process and an important factor in disease pathogenesis. However, its exploitation as a clinical target has achieved limited success and novel molecular targets are required. Although heme oxygenase-1 (HO-1) acts downstream of vascular endothelial growth factor (VEGF) to modulate angiogenesis, knowledge of the mechanisms involved remains limited. We set out identify novel HO-1 targets involved in angiogenesis. HO-1 depletion attenuated VEGF-induced human endothelial cell (EC) proliferation and tube formation...
July 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27299463/synergistic-cytotoxicity-of-ampelopsin-sodium-and-carboplatin-in-human-non-small-cell-lung-cancer-cell-line-spc-a1-by-g1-cell-cycle-arrested
#19
Li Lu, Li-Ning Yang, Xue-Xi Wang, Chun-Li Song, Hong Qin, Yong-Jie Wu
OBJECTIVE: To evaluate the cytotoxic effects of ampelopsin sodium (Amp-Na) and carboplatin (CBP) used alone or in combination on human non-small cell lung cancer (NSCLC) cells SPC-A1 in vitro and its related mechanism. METHODS: Cytotoxic effects were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. The synergistic effects of the drugs were calculated with coeffifi cient of drug interaction (CDI). Cell cycle was determined by flfl ow cytometry (FCM)...
June 14, 2016: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/27295628/atheroprotective-laminar-flow-inhibits-hippo-pathway-effector-yap-in-endothelial-cells
#20
Suowen Xu, Marina Koroleva, Meimei Yin, Zheng Gen Jin
Atherosclerosis is a mechanobiology-related disease that preferentially develops in the aortic arch and arterial branches, which are exposed to disturbed/turbulent blood flow but less in thoracic aorta where the flow pattern is steady laminar flow (LF). Increasing evidence supports that steady LF with high shear stress is protective against atherosclerosis. However, the molecular mechanisms of LF-mediated atheroprotection remain incompletely understood. Hippo/YAP (yes-associated protein) pathway senses and effects mechanical cues and has been reported to be a master regulator of cell proliferation, differentiation, and tissue homeostasis...
October 2016: Translational Research: the Journal of Laboratory and Clinical Medicine
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