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https://www.readbyqxmd.com/read/27978927/-zinc-finger-e-box-binding-homeobox-2-inhibits-hepatitis-b-virus-replication-and-expression
#1
X P Li, Q Hu, Q He, W X Chen
Objective: To investigate the effect of zinc finger E-box-binding homeobox 2 (ZEB2) on hepatitis B virus (HBV) replication and expression. Methods: HepG2, HepG2.2.15, and HepAD38 cells were cultured separately, and Western blot was used to measure the expression of ZEB2. HepG2.2.15 cells were cultured and transfected with ZEB2 expression plasmids or shRNA targeting ZEB2. Western blot was used to measure the expression of ZEB2 and HBV core proteins, quantitative real-time PCR was used to measure HBV 3.5 kb RNA and HBV DNA, Southern blot was used to measure HBV replicative intermediate, and ELISA was used to measure the expression of HBsAg and HBeAg, in order to clarify the effect of ZEB2 on HBV replication and expression...
November 20, 2016: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/27975313/immunofluorescent-staining-for-the-detection-of-the-hepatitis-b-core-antigen-in-frozen-liver-sections-of-human-liver-chimeric-mice
#2
Lena Allweiss, Marc Lütgehetmann, Maura Dandri
The hepatitis B virus (HBV) is the causative agent for chronic hepatitis B infection, which affects an estimate of 240 million people worldwide and puts them at risk of developing terminal liver disease. The life cycle of the virus and its interactions with the host immune system are still incompletely understood, and currently available treatment options rarely achieve a cure. Therefore, basic research and new drug development are needed. One parameter for measuring the intrahepatic activity of the virus is monitoring the production of the HBV core antigen (HBcAg), which not only serves as the main structural protein of its nucleocapsid but is also recruited to the covalently closed circular DNA (cccDNA), the nuclear HBV genome responsible for infection persistence...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27975307/a-homokaryon-assay-for-nucleocytoplasmic-shuttling-activity-of-hbv-core-protein
#3
Ching-Chun Yang, Hung-Cheng Li, Chiaho Shih
Hepatitis B virus (HBV) core protein (HBc) can be present in both nucleus and cytoplasm. The arginine-rich domain (ARD) at the cytoplasmic tail of HBc contains both a nuclear localization signal (NLS) and nuclear export signal (NES). We established a homokaryon assay to detect the dynamic trafficking of HBc between nucleus and cytoplasm in hepatocytes. Using immunofluorescence assay (IFA) and PEG-induced cell-cell fusion, we demonstrated that a chimeric reporter protein of SV40 large T antigen, when fused in-frame with HBc ARD, can shuttle from a donor nuclei (green) to the recipient nuclei (red) in the context of binucleated or polynucleated hybrid cells...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27958343/hbv-maintains-electrostatic-homeostasis-by-modulating-negative-charges-from-phosphoserine-and-encapsidated-nucleic-acids
#4
Pei-Yi Su, Ching-Jen Yang, Tien-Hua Chu, Chih-Hsu Chang, Chiayn Chiang, Fan-Mei Tang, Chih-Yin Lee, Chiaho Shih
Capsid assembly and stability of hepatitis B virus (HBV) core protein (HBc) particles depend on balanced electrostatic interactions between encapsidated nucleic acids and an arginine-rich domain (ARD) of HBc in the capsid interior. Arginine-deficient ARD mutants preferentially encapsidated spliced viral RNA and shorter DNA, which can be fully or partially rescued by reducing the negative charges from acidic residues or serine phosphorylation of HBc, dose-dependently. Similarly, empty capsids without RNA encapsidation can be generated by ARD hyper-phosphorylation in insect, bacteria, and human hepatocytes...
December 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27956427/synergistic-interactions-between-hepatitis-b-virus-ribonuclease-h-antagonists-and-other-inhibitors
#5
Elena Lomonosova, Adam Zlotnick, John E Tavis
Combination therapies are standard for management of human immunodeficiency virus (HIV) and hepatitis C virus (HCV) infections, however no such therapies are established for human hepatitis B virus (HBV). Recently we identified several promising inhibitors of HBV ribonuclease H (RNaseH) activity that have significant activity against viral replication in vitro Here, we investigated in vitro antiviral efficacy of combinations of two RNaseH inhibitors with the current anti-HBV drug nucleoside analog lamivudine, with HAP12, an experimental core protein allosteric modulator, and with each other...
December 12, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27917199/molecular-and-serological-detection-of-occult-hepatitis-b-virus-among-healthy-hepatitis-b-surface-antigen-negative-blood-donors-in-malaysia
#6
Shuaibu A Hudu, Nabil S Harmal, Mohammed I Saeed, Ahmad S Alshrari, Yasmin A Malik, Mohd T Niazlin, Roshida Hassan, Zamberi Sekawi
BACKGROUND: Occult hepatitis B infections are becoming a major global threat, but the available data on its prevalence in various parts of the world are often divergent. OBJECTIVE: This study aimed to detect occult hepatitis B virus in hepatitis B surface antigen-negative serum using anti-HBc as a marker of previous infection. PATIENT AND METHODS: A total of 1000 randomly selected hepatitis B surface antigen-negative sera from blood donors were tested for hepatitis B core antibody and hepatitis B surface antibody using an ELISA and nested polymerase chain reaction was done using primers specific to the surface gene (S-gene)...
September 2016: African Health Sciences
https://www.readbyqxmd.com/read/27882067/bioinformatic-identification-of-rare-codon-clusters-rccs-in-hbv-genome-and-evaluation-of-rccs-in-proteins-structure-of-hepatitis-b-virus
#7
Mojtaba Mortazavi, Mohammad Zarenezhad, Saeid Gholamzadeh, Seyed Moayed Alavian, Mohammad Ghorbani, Reza Dehghani, Abdorrasoul Malekpour, Mohammadhasan Meshkibaf, Ali Fakhrzad
BACKGROUND: Hepatitis B virus (HBV) as an infectious disease that has nine genotypes (A - I) and a 'putative' genotype J. OBJECTIVES: The aim of this study was to identify the rare codon clusters (RCC) in the HBV genome and to evaluate these RCCs in the HBV proteins structure. METHODS: For detection of protein family accession numbers (Pfam) in HBV proteins, the UniProt database and Pfam search tool were used. Protein family accession numbers is a comprehensive and accurate collection of protein domains and families...
October 2016: Hepatitis Monthly
https://www.readbyqxmd.com/read/27864147/host-factor-prpf31-is-involved-in-cccdna-production-in-hbv-replicating-cells
#8
Wataru Kinoshita, Naoki Ogura, Koichi Watashi, Takaji Wakita
Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) plays a central role in chronic HBV infection and replication, and is an important factor for HBV surface antigen loss indicating the endpoint of HBV treatment. However, there is a known problem that current anti-HBV drugs, including interferons and nucleos(t)ide analogues, reduce HBV replication but have a little or no effect on reducing cccDNA. Therefore, the development of new therapeutic agents is necessary to eradicate cccDNA. In this study, we identified pre-mRNA processing factor 31 (PRPF31) by siRNA screening as a factor associated with cccDNA...
January 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27779207/hbv-core-promoter-mutations-and-akt-upregulate-s-phase-kinase-associated-protein-2-to-promote-postoperative-hepatocellular-carcinoma-progression
#9
Lubiao Chen, Lin Gu, Yurong Gu, Hongbo Wang, Meihai Deng, Zania Stamataki, Ye Htun Oo, Yuehua Huang
Mutations in the hepatitis B virus (HBV) core promoter (CP) have been shown to be associated with hepatocellular carcinoma (HCC). The CP region overlaps HBV X gene, which activates AKT to regulate hepatocyte survival. However, the cooperation between these two cascades in HCC progression remains poorly understood. Here, we assayed virological factors and AKT expression in liver tissues from 56 HCC patients with better prognoses (BHCC, ≥5-year survival) and 58 with poor prognoses (PHCC, <5-year survival) after partial liver resection...
October 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27771945/binding-and-uptake-into-human-hepatocellular-carcinoma-cells-of-peptide-functionalized-gold-nanoparticles
#10
Satadru Jha, Federico Ramadori, Santina Quarta, Alessandra Biasiolo, Enrica Fabris, Paola Baldan, Gaetano Guarino, Mariagrazia Ruvoletto, Gianmarco Villano, Cristian Turato, Angelo Gatta, Fabrizio Mancin, Patrizia Pontisso, Paolo Scrimin
One of the most daunting challenges of nanomedicine is the finding of appropriate targeting agents to deliver suitable payloads precisely to cells affected by malignancies. Even more complex is the ability to ensure that the nanosystems enter those cells. Here, we use 2 nm (metal core) gold nanoparticles to target human hepatocellular carcinoma (HepG2) cells stably transfected with the SERPINB3 (SB3) protein. The nanoparticles were coated with a 85:15 mixture of thiols featuring, respectively, a phosphoryl choline (to ensure water solubility and biocompatibility) and a 28-mer peptide corresponding to the amino acid sequence 21-47 of the hepatitis B virus-PreS1 protein (PreS1(21-47))...
January 18, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/27761438/treatment-for-hepatitis-b-in-patients-with-drug-resistance
#11
REVIEW
Frank Tacke, Daniela C Kroy
Persistent hepatitis B virus (HBV) infections affect about 240 million patients worldwide that are at risk of developing liver cirrhosis or hepatocellular carcinoma. HBV is a small, partially double stranded DNA virus with four overlapping genes and a unique life cycle, which involves the generation of an RNA template for replication via reverse transcription. Mutations occur frequently during chronic infection, and particular selection pressures select distinct mutants. Nucleoside and nucleotide analogues like lamivudine (LMV), entecavir (ETV), telbivudine (LdT), adefovir dipivoxil (ADV) and tenofovir (TDF) are used to achieve long-term suppression of viral replication...
September 2016: Annals of Translational Medicine
https://www.readbyqxmd.com/read/27724895/hepatitis-b-virus-inhibits-apolipoprotein-a5-expression-through-its-core-gene
#12
Chengliang Zhu, Guosheng Gao, Hui Song, Fengxia Xu, Kailang Wu, Xinghui Liu
BACKGROUND: Hepatitis B virus (HBV) infection causes lipid metabolism disorders. Apolipoprotein A5 (ApoA5) is a new apolipoprotein family member that plays an important role in the regulation of lipid metabolism. The present study was to investigate the impact of HBV on ApoA5 expression and its regulatory mechanism. METHODS: Reverse transcription polymerase chain reaction (RT-PCR) and western blotting were used to measure ApoA5 mRNA and protein expression in HepG2 and HepG2...
October 10, 2016: Lipids in Health and Disease
https://www.readbyqxmd.com/read/27672298/different-pre-s-deletion-patterns-and-their-association-with-hepatitis-b-virus-genotypes
#13
Bing-Fang Chen
AIM: To investigate the associations of different types of pre-S deletions with hepatitis B virus (HBV) genotypes. METHODS: The sequences of the pre-S region, basal core promoter (BCP) mutation, and precore (PC) mutation were examined through direct DNA sequencing or clonal analysis and sequencing in 273 HBV carriers, namely 55 asymptomatic carriers, 55 carriers with chronic hepatitis (CH), 55 with liver cirrhosis (LC), 53 with liver cirrhotic hepatocellular carcinoma (LC-HCC), and 55 with noncirrhotic HCC...
September 21, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27668597/-the-true-story-and-advantages-of-the-famous-hepatitis-b-virus-core-particles-outlook-2016
#14
P Pumpens, E Grens
This review article is a continuation of the paper "Hepatitis B core particles as a universal display model: a structure-function basis for development" written by Pumpens P. and Grens E., ordered by Professor Lev Kisselev and published in FEBS Letters, 1999, 442, 1-6. The past 17 years have strengthened the paper's finding that the human hepatitis B virus core protein, along with other Hepadnaviridae family member core proteins, is a mysterious, multifunctional protein. The core gene of the Hepadnaviridae genome encodes five partially collinear proteins...
July 2016: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/27636324/modulators-of-hbv-capsid-assembly-as-an-approach-to-treating-hepatitis-b-virus-infection
#15
Andrew G Cole
The search for a cure for hepatitis B virus infection extends beyond interferon and the existing polymerase inhibitors, and targets different aspects of the virus life cycle to develop agents that operate by alternative mechanisms. Examples of small molecules that disrupt the encapsidation of pgRNA have been known for some time, but recent advances in the understanding of nucleocapsid formation, how compounds interact with core protein, and the development of drug-like molecules have recently progressed the study of capsid assembly modulators to proof of concept in the clinic with respect to reduction of viral load in chronic HBV patients...
September 13, 2016: Current Opinion in Pharmacology
https://www.readbyqxmd.com/read/27630726/prevalence-of-serologic-hepatitis-b-markers-in-blood-donors-from-puebla-mexico-the-association-of-relatively-high-levels-of-anti-core-antibodies-with-the-detection-of-surface-antigen-and-genomic-dna
#16
Francisca Sosa-Jurado, Nora Hilda Rosas-Murrieta, Belinda Guzman-Flores, Cintia Perez Zempoaltecalt, Ana Patricia Sanchez Torres, Leticia Ramirez Rosete, Maribel Bernal-Soto, Luis Marquez-Dominguez, Daniel Melendez-Mena, Miguel Angel Mendoza Torres, Maria Teresa Lopez Delgado, Julio Reyes-Leyva, Veronica Vallejo-Ruiz, Gerardo Santos-Lopez
BACKGROUND: The hepatitis B virus (HBV) causes chronic hepatitis, hepatic cirrhosis, and hepatocellular carcinoma. Surface antigen (HBsAg) detection is a definitive test that can confirm HBV infection, while the presence of antibodies against the core protein (anti-HBc) suggests either a previous or ongoing infection or occult hepatitis B infection (OBI). OBJECTIVES: The aim of the present study was to determine the prevalence of anti-HBc and HBsAg in blood donors...
June 2016: Hepatitis Monthly
https://www.readbyqxmd.com/read/27591143/identification-of-hydrolyzable-tannins-punicalagin-punicalin-and-geraniin-as-novel-inhibitors-of-hepatitis-b-virus-covalently-closed-circular-dna
#17
Chunlan Liu, Dawei Cai, Lin Zhang, Wei Tang, Ran Yan, Haitao Guo, Xulin Chen
The development of new agents to target HBV cccDNA is urgently needed because of the limitations of current available drugs for treatment of hepatitis B. By using a cell-based assay in which the production of HBeAg is in a cccDNA-dependent manner, we screened a compound library derived from Chinese herbal remedies for inhibitors against HBV cccDNA. Three hydrolyzable tannins, specifically punicalagin, punicalin and geraniin, emerged as novel anti-HBV agents. These compounds significantly reduced the production of secreted HBeAg and cccDNA in a dose-dependent manner in our assay, without dramatic alteration of viral DNA replication...
October 2016: Antiviral Research
https://www.readbyqxmd.com/read/27558941/functional-characterization-of-hepatitis-b-virus-core-promoter-mutants-revealed-transcriptional-interference-among-co-terminal-viral-mrnas
#18
Chaoyang Chen, Haodi Jia, Fei Zhang, Yanli Qin, Li Zong, Quan Yuan, Yongxiang Wang, Ningshao Xia, Jisu Li, Yumei Wen, Shuping Tong
Hepatitis B virus (HBV) has a 3.2 kb circular DNA genome. It employs four promoters in conjunction with a single polyadenylation signal to generate 3.5, 2.4, 2.1 and 0.7 kb co-terminal RNAs. The 3.5 kb RNA is subdivided into the precore RNA for e-antigen expression and pregenomic RNA for genome replication. When introduced to a genotype A clone, several core promoter mutations markedly enhanced HBV genome replication, but suppressed e-antigen expression through up-regulation of pregenomic RNA at the expense of precore RNA...
October 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27553619/hepatitis-b-e-antigen-expression-by-hepatitis-b-virus-subgenotype-a1-relative-to-subgenotypes-a2-and-d3-in-cultured-hepatocellular-carcinoma-huh7-cells
#19
Nimisha Harshadrai Bhoola, Anna Kramvis
BACKGROUND: Hepatitis B virus (HBV) is hyperendemic in southern Africa, with subgenotype A1 prevailing. The precore/core (preC/C) region of A1, encoding for hepatitis B e antigen (HBeAg), has unique sequence characteristics, differentiating it from subgenotypes A2 and D3. Our aim was to follow the expression of HBeAg in vitro by the three subgenotypes. METHODS: Huh7 cells were transfected with plasmids belonging to subgenotypes A1, A2, and D3. Using indirect immunofluorescence, the expression of HBeAg was followed, as was the activation of the unfolded protein response (UPR) and subsequent activation of apoptosis...
2016: Intervirology
https://www.readbyqxmd.com/read/27547127/review-of-laboratory-tests-used-in-monitoring-hepatitis-b-response-to-pegylated-interferon-and-nucleos-t-ide-analog-therapy
#20
Carla Osiowy, Carla Coffin, Anton Andonov
There are only two currently approved classes of hepatitis B virus (HBV) antiviral agents, pegylated interferon (Peg-IFN), and nucleos(t)ide analogs (NAs) for chronic HBV infection. Although Peg-IFN is used for a finite 48-week duration and offers a greater chance of sustained off-treatment virological response, it is poorly tolerated and can only be offered to selected patients. The NAs are well tolerated but require prolonged therapy due to risk of relapse with treatment cessation. There is evolving data that novel virological assays (e...
2016: Current Treatment Options in Infectious Diseases
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