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https://www.readbyqxmd.com/read/28224115/the-escherichia-coli-serogroup-o1-and-o2-lipopolysaccharides-are-encoded-by-multiple-o-antigen-gene-clusters
#1
Sabine Delannoy, Lothar Beutin, Patricia Mariani-Kurkdjian, Aubin Fleiss, Stéphane Bonacorsi, Patrick Fach
Escherichia coli strains belonging to serogroups O1 and O2 are frequently associated with human infections, especially extra-intestinal infections such as bloodstream infections or urinary tract infections. These strains can be associated with a large array of flagellar antigens. Because of their frequency and clinical importance, a reliable detection of E. coli O1 and O2 strains and also the frequently associated K1 capsule is important for diagnosis and source attribution of E. coli infections in humans and animals...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28221838/use-of-the-escherichia-coli-identification-microarray-for-characterizing-the-health-risks-of-shiga-toxin-producing-escherichia-coli-isolated-from-foods
#2
David W Lacher, Jayanthi Gangiredla, Isha Patel, Christopher A Elkins, Peter C H Feng
More than 470 serotypes of Shiga toxin-producing Escherichia coli (STEC) have been identified, but not all cause severe illness in humans. Most STEC that cause severe diseases can adhere to epithelial cells, produce specific stx subtypes, and belong to certain serotypes; therefore, these traits appear to be critical STEC risk factors. However, testing for these traits is labor intensive, and serotyping is inadequate because of extensive variations among E. coli O and H antigen types. In the present study, the E...
October 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28218480/human-leucocyte-antigen-hla-dqb1-03-02-and-hla-a-02-01-have-opposite-patterns-in-their-effects-on-susceptibility-to-hiv-infection
#3
N Rallón, C Restrepo, J L Vicario, J Del Romero, C Rodríguez, J García-Samaniego, M García, A Cabello, M Górgolas, J M Benito
OBJECTIVES: The objective of this study was to seek correlates of immune protection in HIV infection. We sought to elucidate the association between the presence of human leucocyte antigen (HLA) alleles, as well as killer immunoglobulin receptor (KIR) genotypes, and the susceptibility to HIV infection in a Spanish cohort of HIV-exposed seronegative (HESN) individuals. METHODS: A total of 152 individuals were evaluated: 29 HESN individuals in stable heterosexual relationships with an HIV-infected partner admitting high-risk sexual intercourse for at least 12 months prior to inclusion in the study, 61 HIV-infected patients and 62 healthy controls...
February 20, 2017: HIV Medicine
https://www.readbyqxmd.com/read/28214743/tio2-nanowire-arrays-modified-with-a-simultaneous-etching-doping-and-deposition-technique-for-ultrasensitive-amperometric-immunosensing
#4
Xiaoqiang Liu, Xiaohe Huo, Peipei Liu, Yunfei Tang, Jun Xu, Huangxian Ju
In this work, an ultrasensitive immunosensing scaffold was structured with TiO2 nanowire (TiNW) arrays modified with molybdenum (Mo) and MoS2 flakes by a triplex "etching, doping and deposition" technique. The triply modification of TiNW arrays improved their electron transfer, and the decoration of MoS2 flakes on TiNW arrays increased both the conductivity and the specific surface area of TiNW. Accordingly, the triply modified TiNW arrays provided a biocompatible microenviroment for the biomolecules and high specific surface area to load big amount of biomolecules...
February 14, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28187738/naturally-occurring-anti-glycan-antibodies-binding-to-globo-h-expressing-cells-identify-ovarian-cancer-patients
#5
Tatiana Pochechueva, Shahidul Alam, Andreas Schötzau, Alexander Chinarev, Nicolai V Bovin, Neville F Hacker, Francis Jacob, Viola Heinzelmann-Schwarz
BACKGROUND: Glycosphingolipids are important compounds of the plasma membrane of mammalian cells and a number of them have been associated with malignant transformation and progression, reinforcing tumour aggressiveness and metastasis. Here we investigated the levels of naturally occurring anti-glycan antibodies to Globo H in blood plasma obtained from high-grade serous ovarian cancer patients (SOC) and women without gynaecological malignancies (control) using suspension glycan array technology employing chemically synthesized glycans as antibody targets...
February 10, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28183334/circulating-plasmablasts-plasma-cells-a-potential-biomarker-for-igg4-related-disease
#6
Wei Lin, Panpan Zhang, Hua Chen, Yu Chen, Hongxian Yang, Wenjie Zheng, Xuan Zhang, Fengxiao Zhang, Wen Zhang, Peter E Lipsky
BACKGROUND: Immunoglobulin G4 (IgG4)-related disease (IgG4-RD) is a multisystem fibroinflammatory disease. We previously reported that a circulating cell population expressing CD19(+)CD24(-)CD38(hi) was increased in patients with IgG4-RD. In this study, we aimed to document that this cell population represented circulating plasmablasts/plasma cells, to identify the detailed phenotype and gene expression profile of these IgG4-secreting plasmablasts/plasma cells, and to determine whether this B-cell lineage subset could be a biomarker in IgG4-related disease (IgG4-RD)...
February 10, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28183299/avidity-of-anti-malarial-antibodies-inversely-related-to-transmission-intensity-at-three-sites-in-uganda
#7
Isaac Ssewanyana, Emmanuel Arinaitwe, Joaniter I Nankabirwa, Adoke Yeka, Richard Sullivan, Moses R Kamya, Philip J Rosenthal, Grant Dorsey, Harriet Mayanja-Kizza, Chris Drakeley, Bryan Greenhouse, Kevin K A Tetteh
BACKGROUND: People living in malaria endemic areas acquire protection from severe malaria quickly, but protection from clinical disease and control of parasitaemia is acquired only after many years of repeated infections. Antibodies play a central role in protection from clinical disease; however, protective antibodies are slow to develop. This study sought to investigate the influence of Plasmodium falciparum exposure on the acquisition of high-avidity antibodies to P. falciparum antigens, which may be associated with protection...
February 10, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28167275/single-amino-acid-fingerprinting-of-the-human-antibody-repertoire-with-high-density-peptide-arrays
#8
Laura K Weber, Andrea Palermo, Jonas Kügler, Olivier Armant, Awale Isse, Simone Rentschler, Thomas Jaenisch, Jürgen Hubbuch, Stefan Dübel, Alexander Nesterov-Mueller, Frank Breitling, Felix F Loeffler
The antibody species that patrol in a patient's blood are an invaluable part of the immune system. While most of them shield us from life-threatening infections, some of them do harm in autoimmune diseases. If we knew exactly all the antigens that elicited all the antibody species within a group of patients, we could learn which ones correlate with immune protection, are irrelevant, or do harm. Here, we demonstrate an approach to this question: First, we use a plethora of phage-displayed peptides to identify many different serum antibody binding peptides...
February 3, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28166716/pyhla-tests-for-the-association-between-hla-alleles-and-diseases
#9
Yanhui Fan, You-Qiang Song
BACKGROUND: Recently, several tools have been designed for human leukocyte antigen (HLA) typing using single nucleotide polymorphism (SNP) array and next-generation sequencing (NGS) data. These tools provide high-throughput and cost-effective approaches for identifying HLA types. Therefore, tools for downstream association analysis are highly desirable. Although several tools have been designed for multi-allelic marker association analysis, they were designed only for microsatellite markers and do not scale well with increasing data volumes, or they were designed for large-scale data but provided a limited number of tests...
February 6, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28164316/first-description-of-a-d-ce-d-hybrid-gene-on-a-weak-d-type-2-molecular-background
#10
Thomas Granier, Jacques Chiaroni, Pascal Bailly, Monique Silvy
BACKGROUND: RhD phenotypes that express a significantly reduced amount of RhD antigen per red blood cell may be mistyped as RhD-negative by standard serologic methods. The molecular identification of weak D Type 1, 2, or 3 carriers allows managing them as RhD-positive and, thus, rationalizes the use of RhD-negative stock units and the administration of Rh-immunoglobulin prophylaxis, avoiding unnecessary costs and possible side effects. STUDY DESIGN AND METHODS: One sample was investigated for confirming a D-C-E+c+e- phenotype...
February 5, 2017: Transfusion
https://www.readbyqxmd.com/read/28163018/multiplexed-fc-array-for-evaluation-of-antigen-specific-antibody-effector-profiles
#11
Eric P Brown, Karen G Dowell, Austin W Boesch, Erica Normandin, Alison E Mahan, Thach Chu, Dan H Barouch, Chris Bailey-Kellogg, Galit Alter, Margaret E Ackerman
Antibodies are widely considered to be a frequent primary and often mechanistic correlate of protection of approved vaccines; thus evaluating the antibody response is of critical importance in attempting to understand and predict the efficacy of novel vaccine candidates. Historically, antibody responses have been analyzed by determining the titer of the humoral response using measurements such as an ELISA, neutralization, or agglutination assays. In the simplest case, sufficiently high titers of antibody against vaccine antigen(s) are sufficient to predict protection...
February 2, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28143425/higher-igfbp-1-to-igf-1-serum-ratio-predicts-unfavourable-survival-in-patients-with-nasopharyngeal-carcinoma
#12
Xinwei Feng, Jianhua Lin, Shan Xing, Wanli Liu, Ge Zhang
BACKGROUND: The insulin-like growth factor (IGF) system plays an important role in the development and progression of cancer. However, little is known about the expression of the IGF system components and their clinicopathological significance and prognostic value in nasopharyngeal carcinoma (NPC). METHODS: IGF system components (IGF-1, IGF-2, IGF-1SR, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4 and IGFBP-6) were quantified from the plasma of NPC patients and healthy individuals using the RayBio Human Cytokine Antibody Array...
January 31, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28131394/novel-adenovirus-encoded-virus-like-particles-displaying-the-placental-malaria-associated-var2csa-antigen
#13
Anne-Marie C Andersson, Mafalda Resende, Ali Salanti, Morten A Nielsen, Peter J Holst
The malaria parasite Plasmodium falciparum presents antigens on the infected erythrocyte surface that bind human receptors expressed on the vascular endothelium. The VAR2CSA mediated binding to a distinct chondroitin sulphate A (CSA) is a crucial step in the pathophysiology of placental malaria and the CSA binding region of VAR2CSA has been identified as a promising vaccine target against placental malaria. Here we designed adenovirus encoded virus-like particles (VLP) by co-encoding Simian Immunodeficiency Virus (SIV) gag and VAR2CSA...
January 25, 2017: Vaccine
https://www.readbyqxmd.com/read/28125011/microshell-arrays-enhanced-sensitivity-in-detection-of-specific-antibody-for-reduced-graphene-oxide-optical-sensor
#14
Wen-Shuai Jiang, Wei Xin, Shao-Nan Chen, Cun-Bo Li, Xiao-Guang Gao, Lei-Ting Pan, Zhi-Bo Liu, Jian-Guo Tian
Protein-protein interactions play an important role in the investigation of biomolecules. In this paper, we reported on the use of a reduced graphene oxide microshell (RGOM)-based optical biosensor for the determination of goat anti-rabbit IgG. The biosensor was prepared through a self-assembly of monolayers of monodisperse polystyrene microspheres, combined with a high-temperature reduction, in order to decorate the RGOM with rabbit IgG. The periodic microshells allowed a simpler functionalization and modification of RGOM with bioreceptor units, than reduced graphene oxide (RGO)...
January 24, 2017: Sensors
https://www.readbyqxmd.com/read/28109682/label-free-checkerboard-assay-to-determine-overlapping-epitopes-of-ebola-virus-vp-40-antibodies-using-surface-plasmon-resonance
#15
George P Anderson, Jinny L Liu, Dan Zabetakis, Patricia M Legler, Ellen R Goldman
Immunoassay formats, in which antibodies provide sensitivity and specificity, are often utilized to provide rapid and simple diagnostic tests. Surface plasmon resonance is frequently used to evaluate the suitability of antibodies by determining binding kinetics to agents or surrogate antigens. We used SPR to evaluate a number of commercial monoclonal antibodies as well as single domain antibodies produced in-house. All the antibodies targeted the Ebola virus viral protein 40 (VP40). We determined the ability of each antibody to bind to immobilized VP40, and ensured they did not bind Ebola glycoprotein or the nucleoprotein...
January 19, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28107606/dishevelled-segment-polarity-protein-3-dvl3-a-novel-and-easily-applicable-recurrence-predictor-in-localized-prostate-adenocarcinoma
#16
Pil-Jong Kim, Ji Y Park, Hong-Gee Kim, Yong Mee Cho, Heounjeong Go
OBJECTIVE: To identify new biomarkers for the biochemical recurrence (BCR) of prostate adenocarcinoma. PATIENTS AND METHODS: Clinical information of 500 prostate adenocarcinoma patients and their 152 RNA-sequencing and protein-array data from The Cancer Genome Atlas (TCGA) were separated into a discovery-set and a validation-set. Each dataset was analyzed according to the Gleason grade groups reflecting BCR. The results obtained from the analysis using TCGA dataset were confirmed by immunohistochemistry analyses of the confirmation cohort composed of 395 localized prostate adenocarcinoma patients...
January 20, 2017: BJU International
https://www.readbyqxmd.com/read/28092639/lithographic-patterning-of-antibodies-by-direct-lift-off-and-improved-surface-adhesion
#17
Sankalp Verma, Vivek Verma
The inherent property of antibodies binding to their antigen with high specificity makes them a strong candidate for sensing and detection applications. Microscale patterning of antibodies is desired for the miniaturization of sensors and fundamental cell biology studies. However, existing methodologies to pattern antibodies at the microscale are multi-step. In this work, we demonstrate microscale patterning of antibodies on a glass coverslip in a single step photolithography process. The microscale features of the photoresist were generated on the coverslip using photolithography, and the antibody solution was incubated...
January 16, 2017: Biofabrication
https://www.readbyqxmd.com/read/28090561/diversity-in-rotavirus-host-glycan-interactions-a-sweet-spectrum
#18
REVIEW
Sasirekha Ramani, Liya Hu, B V Venkataram Prasad, Mary K Estes
Interaction with cellular glycans is a critical initial step in the pathogenesis of many infectious agents. Technological advances in glycobiology have expanded the repertoire of studies delineating host glycan-pathogen interactions. For rotavirus, the VP8* domain of the outer capsid spike protein VP4 is known to interact with cellular glycans. Sialic acid was considered the key cellular attachment factor for rotaviruses for decades. Although this is true for many rotavirus strains causing infections in animals, glycan array screens show that many human rotavirus strains bind nonsialylated glycoconjugates, called histo-blood group antigens, in a strain-specific manner...
May 2016: Cellular and Molecular Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28067370/automated-3d-printed-unibody-immunoarray-for-chemiluminescence-detection-of-cancer-biomarker-proteins
#19
C K Tang, A Vaze, J F Rusling
A low cost three-dimensional (3D) printed clear plastic microfluidic device was fabricated for fast, low cost automated protein detection. The unibody device features three reagent reservoirs, an efficient 3D network for passive mixing, and an optically transparent detection chamber housing a glass capture antibody array for measuring chemiluminescence output with a CCD camera. Sandwich type assays were built onto the glass arrays using a multi-labeled detection antibody-polyHRP (HRP = horseradish peroxidase)...
January 31, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28058210/biology-of-chronic-graft-vs-host-disease-immune-mechanisms-and-progress-in-biomarker-discovery
#20
REVIEW
Richard B Presland
Chronic graft-vs-host disease (cGVHD) is the leading cause of long-term morbidity and mortality following allogeneic hematopoietic stem cell transplantation. It presents as a chronic inflammatory and sclerotic autoimmune-like condition that most frequently affects the skin, oral mucosa, liver, eyes and gastrointestinal tract. Both clinical and animal studies have shown that multiple T cell subsets including Th1, Th2, Th17, T follicular helper cells and regulatory T-cells play some role in cGVHD development and progression; B cells also play an important role in the disease including the production of antibodies to HY and nuclear antigens that can cause serious tissue damage...
December 24, 2016: World Journal of Transplantation
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