Jackson Alves da Silva Queiroz, Tárcio Peixoto Roca, Rutilene Barbosa Souza, Luiz Fellype Alves de Souza, Ana Maísa Passos-Silva, André Luiz Ferreira da Silva, Eugênia de Castro E Silva, Lourdes Maria Pinheiro Borzacov, Rita de Cássia Pontello Rampazzo, Soraya Dos Santos Pereira, Thor Oliveira Dantas, Janaína Mazaro, Lívia Melo Villar, Juan Miguel Villalobos Salcedo, Daniel Archimedes da Matta, Deusilene Vieira
Hepatitis Delta is a disease caused by exposure to hepatitis B (HBV) and hepatitis D (HDV) viruses, usually with a more severe clinical outcome when compared to an HBV monoinfection. To date, the real prevalence of HDV infection is underestimated and detection methods are poorly available, especially in more endemic regions. Therefore, a one-step RT-qPCR method for quantification of HDV-RNA was developed. Biological samples were selected between 2017 and 2023 from patients at the Ambulatório Especializado em Hepatites Virais of the Centro de Pesquisa em Medicina Tropical de Rondônia and Serviço de Assistência Especializada and underwent the test developed by this study and a second quantitative RT-qPCR assay...
July 26, 2023: Scientific Reports