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https://www.readbyqxmd.com/read/28738346/synergistic-activity-of-berberine-with-azithromycin-against-pseudomonas-aeruginosa-isolated-from-patients-with-cystic-fibrosis-of-lung-in-vitro-and-in-vivo
#1
YongTao Li, JianRong Huang, LanJuan Li, LinSheng Liu
BACKGROUND/AIMS: Pseudomonas aeruginosa (PA) is one of the major opportunistic pathogens which can cause chronic lung infection of cystic fibrosis (CF). The formation of PA biofilm promotes CF development and restricts the antimicrobial efficacies of current antibiotics. METHODS: The antimicrobial effects of azithromycin (AZM) and berberine (BER) alone and in combination were evaluated using microdilution method, checkerboard assay, time-kill test, qRT-PCR analysis and absorption method...
July 24, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28737721/pyranopyran-1-8-dione-an-active-compound-from-vitices-fructus-attenuates-cigarette-smoke-induced-lung-inflammation-in-mice
#2
Gihyun Lee, Kyung-Hwa Jung, Eun Seok Ji, Hyunsu Bae
Previously, we isolated and identified pyranopyran-1,8-dione (PPY) from Viticis Fructus, as a bioactive compound possessing anti-inflammatory properties. The present study was aimed to evaluate the preventive benefit of PPY on cigarette-smoke (CS)-induced lung inflammation. C57BL/6 mice were exposed to CS for 2 weeks while PPY was administrated by oral injection 2 h before CS exposure. To validate the anti-inflammatory effects of PPY, the numbers of immune cells in the bronchoalveolar lavage fluid were counted...
July 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28735722/-is-the-introduction-of-clinical-management-programs-for-patients-with-chronic-obstructive-pulmonary-disease-useful-comparison-of-the-effectiveness-of-two-interventions-on-the-clinical-progress-and-care-received
#3
Ingrid Solanes, Ignasi Bolíbar, Maria Antònia Llauger, Meritxell Peiro, Pepi Valverde, Mar Fraga, Casimira Medrano, Teresa Bigorra, Montserrat Freixas, Iskra Ligüerre, Maria Antònia Pou, Leandra Domínguez, Carles Valero, Judit Solà, Jordi Giner, Vicente Plaza
OBJECTIVE: To evaluate the effectiveness of two management programs on patients with chronic obstructive pulmonary disease (COPD). DESIGN: A study with a quasi-experimental design was used to evaluate the effectiveness of two interventions (I1, I2) for the care of patients with COPD after a mean follow-up of 31.2months. SETTING: Primary Care Centres in two Barcelona Health Areas and their referral hospitals. PARTICIPANTS: Patients with COPD selected by simple random sampling using any disease code corresponding to COPD...
July 20, 2017: Atencion Primaria
https://www.readbyqxmd.com/read/28734862/respiratory-infections-and-antibiotic-usage-in-common-variable-immunodeficiency
#4
Johannes M Sperlich, Bodo Grimbacher, Sarita Workman, Tanzina Haque, Suranjith L Seneviratne, Siobhan O Burns, Veronika Reiser, Werner Vach, John R Hurst, David M Lowe
BACKGROUND: Patients with common variable immunodeficiency (CVID) suffer frequent respiratory tract infections despite immunoglobulin replacement and are prescribed significant quantities of antibiotics. The clinical and microbiological nature of these exacerbations, the symptomatic triggers to take antibiotics, and the response to treatment have not been previously investigated. OBJECTIVES: To describe the nature, frequency, treatment, and clinical course of respiratory tract exacerbations in patients with CVID and to describe pathogens isolated during respiratory tract exacerbations...
July 19, 2017: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/28734808/-congenital-cytomegalovirus-infection-manifesting-as-neonatal-respiratory-distress-in-an-hiv-exposed-uninfected-newborn
#5
A Pham, H El Mjati, N Nathan, F Kieffer, D Mitanchez
BACKGROUND: Cytomegalovirus (CMV) is one of the most common intrauterine infections, affecting approximately 1% of all live births. There are few reports on congenital CMV infections manifesting as isolated pneumonitis. CASE REPORT: We report a case of congenital CMV with neonatal respiratory distress affecting an HIV-exposed uninfected infant. This infant required noninvasive ventilation beginning within the first 15min of life. The initial chest X-ray showed diffuse bilateral ground-glass opacifications...
July 19, 2017: Archives de Pédiatrie: Organe Officiel de la Sociéte Française de Pédiatrie
https://www.readbyqxmd.com/read/28734369/systematic-comparison-of-exosomal-proteomes-from-human-saliva-and-serum-for-the-detection-of-lung-cancer
#6
Yan Sun, Sha Liu, Zhi Qiao, Zhi Shang, Zhijun Xia, Xiaomin Niu, Liqiang Qian, Yan Zhang, Liuyin Fan, Cheng-Xi Cao, Hua Xiao
Circulating tumor exosomes harbor plenty of cancer biological information, which have emerged as promising targets for cancer early detection and diagnosis. Human serum and saliva are unique diagnostic body fluids, which contain numerous circulating exosomes. It is necessary to establish standardized isolation method and compare their proteome profiling for translational medicine. High abundant proteins in these body fluids were removed before exosomes isolation and obtained exosomes were further confirmed by morphology analysis and surface biomarker test...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28733286/the-regulator-ldhr-and-the-d-lactate-dehydrogenase-ldha-of-burkholderia-multivorans-play-a-role-in-carbon-overflow-and-in-planktonic-cellular-aggregates-formation
#7
Inês N Silva, Marcelo J Ramires, Lisa A Azevedo, Ana R Guerreiro, Andreia C Tavares, Jörg D Becker, Leonilde M Moreira
LysR-type transcriptional regulators (LTTR) are the most commonly found regulators in Burkholderia cepacia complex, comprising opportunistic pathogens causing chronic respiratory infections in cystic fibrosis (CF) patients. Despite LTTRs being global regulators of pathogenicity in several bacteria, few have been characterized in Burkholderia Here, we showed that gene ldhR of B. multivorans encoding a LTTR is co-transcribed with ldhA encoding a d-lactate dehydrogenase, and evaluate their implication in virulence traits like exopolysaccharide (EPS) synthesis and biofilm formation...
July 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28733217/protective-effect-of-forsythiaside-a-on-ova-induced-asthma-in-mice
#8
Jin Qian, Xiaorong Ma, Yali Xun, Lei Pan
Forsythiaside A (FSA), an active constituent isolated from the Chinese medicinal herb Forsythia suspensa, has been known to have anti-inflammatory effect. However, the effect of FSA on allergic airway inflammation remains unclear. The aim of this study was to investigate the effect of FSA on OVA-induced asthma in mice. Mice model of asthma was induced by OVA. OVA-induced airway hyperresponsiveness (AHR) and inflammatory cells in BALF were detected. The production of IgE, IL-4, IL-5, IFN-γ, and IL-13 were detected by ELISA...
July 18, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28731226/curcumin-converts-foxp3-regulatory-t-cells-to-t-helper-1-cells-in-patients-with-lung-cancer
#9
JianYong Zou, ChunHua Su, HongHe Luo, YiYan Lei, Bo Zeng, HaoShuai Zhu, ZhenGuang Chen
The regulatory T cells (Treg) play an important role in the tumor tolerance. The methods to regulate the Treg population in cancer-bearing hosts are limited currently. The effect of curcumin on inhibiting cancer has been recognized, but the mechanism remains elusive. This study tests a hypothesis that administration of curcumin down regulates Tregs in lung cancer (LC) patients. In this study, a group of LC patients was treated with curcumin. The peripheral Tregs and T helper (Th) 1 cells were analyzed by flow cytometry...
July 21, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28730484/analysis-of-phosphotyrosine-signaling-networks-in-lung-cancer-cell-lines
#10
Malgorzata Broncel, Paul H Huang
Robust isolation and identification of peptides phosphorylated at their tyrosine residues are key steps in deciphering complex signaling networks governed by protein tyrosine kinases, including kinases involved in oncogenesis. Phosphotyrosine (pY)-containing peptides are commonly isolated from cellular lysates by means of antibody and/or metal affinity-based enrichment followed by their identification by mass spectrometry. Herein, we describe robust two-stage isolation of phosphotyrosine peptides and mass spectrometry-aided identification of phosphosites to characterize basal signaling networks in unstimulated non-small cell lung cancer (NSCLC) cell lines...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28729396/acute-volume-loading-exacerbates-direct-ventricular-interaction-in-a-model-of-copd
#11
William Spencer Cheyne, Alexandra Mackenzie Williams, Megan I Harper, Neil Derek Eves
Volume loading increases left ventricular (LV) stroke volume (LVSV) through series interaction but may paradoxically reduce LVSV in the presence of large increases in right ventricular (RV) afterload due to direct ventricular interaction (DVI). RV afterload is often increased in COPD as a result of pathological changes to respiratory mechanics, namely increased negative intrathoracic pressure (nITP), dynamic lung hyperinflation (DH) and increased pulmonary vascular resistance (PVR). These hallmarks of COPD negatively impact LV hemodynamics in normovolemia...
July 20, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28729229/pharbitis-nil-pn-induces-apoptosis-and-autophagy-in-lung-cancer-cells-and-autophagy-inhibition-enhances-pn-induced-apoptosis
#12
Hyun Jin Jung, Ju-Hee Kang, Seungho Choi, Youn Kyoung Son, Kang Ro Lee, Je Kyung Seong, Sun Yeou Kim, Seung Hyun Oh
ETHNOPHARMACOLOGICAL RELEVANCE: Pharbitis Nil (PN) is used as a main component of the existing drug, DA-9701, which was developed to treat functional dyspepsia (FD) in Korea. PN extracts isolated from its seeds have been reported to have anticancer effects. AIM OF THE STUDY: The purpose of this study was to investigate the underlying mechanism of the chemotherapeutic effects of PN in lung cancer cells. MATERIALS AND METHODS: We performed MTT assays, colony formation assays, flow cytometry assays, Western blot analysis, reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescence analysis, and cell counting assays to study the molecular mechanism of chemotherapeutic effects of PN in lung cancer cells...
July 17, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28729000/airway-microbiota-signals-anabolic-and-catabolic-remodeling-in-the-transplanted-lung
#13
Stéphane Mouraux, Eric Bernasconi, Céline Pattaroni, Angela Koutsokera, John-David Aubert, Johanna Claustre, Christophe Pison, Pierre-Joseph Royer, Antoine Magnan, Romain Kessler, Christian Benden, Paola M Soccal, Benjamin J Marsland, Laurent P Nicod
BACKGROUND: Homeostatic turnover of the extracellular matrix conditions the structure and function of the healthy lung. In lung transplantation, long-term management remains limited by Chronic Lung Allograft Dysfunction (CLAD), an umbrella term used for a heterogeneous entity ultimately associated with pathological airway and/or parenchyma remodeling. OBJECTIVE: To assess whether the local cross-talk between the pulmonary microbiota and host cells is a key determinant in the control of lower airway remodeling post-transplantation...
July 17, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28728277/-effects-of-pi3k%C3%AE-rhoa-pathway-on-phagocytosis-defect-of-alveolar-macrophages-in-a-mouse-model-of-chronic-obstructive-pulmonary-disease
#14
S T Hu, Q Xia, X L Zeng, H R Bao, X J Liu
Objective: To investigate effects of Phosphoinositide3-Kinases (PI3Kδ)-Ras homolog gene family member A(RhoA) pathway on phagocytosis deficiency of alveolar macrophages (AMs) in a mouse model of chronic obstructive pulmonary disease (COPD). Methods: Twenty mice were exposed to cigarette smoking to establish the COPD model, with 20 mice as the control group. AMs were isolated from lung tissue by discontinuous density gradient centrifugation and then divided into a healthy control group, a COPD group, a healthy IC87114 group and a COPD IC87114 group...
July 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28726588/reconstituting-mouse-lungs-with-conditionally-reprogrammed-human-bronchial-epithelial-cells
#15
Ryan LaRanger, Jennifer R Peters-Hall, Melissa Coquelin, Busola R Alabi, Chris Chen, Woodring E Wright, Jerry Shay
We developed methods for conditionally reprogramming (CR) primary human bronchial epithelial cells (HBECs) to extend their functional life span and permit their differentiation into both upper and lower airway lung epithelium. We also developed a bioreactor to support vascular perfusion and rhythmic breathing of decellularized mouse lungs reconstituted with CR HBECs isolated from patients with and without cystic fibrosis (CF). While conditionally reprogrammed cells only differentiate into an upper airway epithelium after 35 days at the air liquid interface, in reconstituted lungs these cells differentiate into upper airway bronchial epithelium and lower airway alveolar structures after 12 days...
July 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28725552/repeated-inoculations-with-the-lung-and-heartworm-nematode-angiostrongylus-vasorum-result-in-increasing-larval-excretion-and-worm-burden-in-the-red-fox-vulpes-vulpes
#16
Ian David Woolsey, P Webster, S Thamsborg, Manuela Schnyder, Jesper Monrad, C M O Kapel
The French heartworm Angiostongylus vasorum is found in European red fox (Vulpes vulpes) and dog populations, where it appears to be spreading geographically. Once introduced into new areas, it establishes in local fox populations, typically to over 50% prevalence in a few years. High susceptibility and constant excretion of first stage larvae (L1) by the definitive hosts are prerequisites for sustaining high parasite biomass in a particular habitat. The present study explores the hypothesis that repeated ingestion of gastropods in nature will result in accumulation of adult worms and elevated excretion of L1 in feces...
December 2017: International Journal for Parasitology. Parasites and Wildlife
https://www.readbyqxmd.com/read/28724778/eupnea-tachypnea-and-autoresuscitation-in-a-closed-loop-respiratory-control-model
#17
Casey O Diekman, Peter J Thomas, Christopher G Wilson
How sensory information influences the dynamics of rhythm generation varies across systems, and general principles for understanding this aspect of motor control are lacking. Determining the origin of respiratory rhythm generation is challenging because the mechanisms in a central circuit considered in isolation may be different than those in the intact organism. We analyze a closed-loop respiratory control model incorporating a central pattern generator (CPG), the Butera-Rinzel-Smith (BRS) model, together with lung mechanics, oxygen handling, and chemosensory components...
July 19, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28723550/induced-pluripotent-stem-cell-derived-primitive-macrophages-provide-a-platform-for-modeling-tissue-resident-macrophage-differentiation-and-function
#18
Kazuyuki Takata, Tatsuya Kozaki, Christopher Zhe Wei Lee, Morgane Sonia Thion, Masayuki Otsuka, Shawn Lim, Kagistia Hana Utami, Kerem Fidan, Dong Shin Park, Benoit Malleret, Svetoslav Chakarov, Peter See, Donovan Low, Gillian Low, Marta Garcia-Miralles, Ruizhu Zeng, Jinqiu Zhang, Chi Ching Goh, Ahmet Gul, Sandra Hubert, Bernett Lee, Jinmiao Chen, Ivy Low, Nurhidaya Binte Shadan, Josephine Lum, Tay Seok Wei, Esther Mok, Shohei Kawanishi, Yoshihisa Kitamura, Anis Larbi, Michael Poidinger, Laurent Renia, Lai Guan Ng, Yochai Wolf, Steffen Jung, Tamer Önder, Evan Newell, Tara Huber, Eishi Ashihara, Sonia Garel, Mahmoud A Pouladi, Florent Ginhoux
Tissue macrophages arise during embryogenesis from yolk-sac (YS) progenitors that give rise to primitive YS macrophages. Until recently, it has been impossible to isolate or derive sufficient numbers of YS-derived macrophages for further study, but data now suggest that induced pluripotent stem cells (iPSCs) can be driven to undergo a process reminiscent of YS-hematopoiesis in vitro. We asked whether iPSC-derived primitive macrophages (iMacs) can terminally differentiate into specialized macrophages with the help of growth factors and organ-specific cues...
July 18, 2017: Immunity
https://www.readbyqxmd.com/read/28723487/clinical-case-discussion-isolated-lung-metastases-after-radical-nephrectomy-for-clear-cell-carcinoma-the-case-for-surgery
#19
Axel S Merseburger, Emanuel Palade
In a stable patient with metastatic renal cell carcinoma, metastasectomy is an option when all the lesions can be resected. Individual informed consent from the patient is most important.
December 15, 2016: European Urology Focus
https://www.readbyqxmd.com/read/28723486/isolated-lung-metastases-after-radical-nephrectomy-for-clear-cell-carcinoma
#20
R Jeroen A van Moorselaar, C Willemien Menke-van der Houven van Oordt
Here we describe a case of a biologically indolent renal cell carcinoma. A single lung metastasis was found 1 yr after resection of a pT2bNxM0 Fuhrmann grade 2 tumor. After a further 1 yr, the patient was treated with IFN-alpha and bevacuzimab. Treatment for renal cell carcinoma should be tailored individually for each patient.
December 15, 2016: European Urology Focus
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