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https://www.readbyqxmd.com/read/28526890/a-heteromeric-molecular-complex-regulates-the-migration-of-lung-alveolar-epithelial-cells-during-wound-healing
#1
Manik C Ghosh, Patrudu S Makena, Joseph Kennedy, Bin Teng, Charlean Luellen, Scott E Sinclair, Christopher M Waters
Alveolar type II epithelial cells (ATII) are instrumental in early wound healing in response to lung injury, restoring epithelial integrity through spreading and migration. We previously reported in separate studies that focal adhesion kinase-1 (FAK) and the chemokine receptor CXCR4 promote epithelial repair mechanisms. However, potential interactions between these two pathways were not previously considered. In the present study, we found that wounding of rat ATII cells promoted increased association between FAK and CXCR4...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28526615/lung-inflammation-after-bleomycin-treatment-in-mice-selection-of-an-accurate-normalization-strategy-for-gene-expression-analysis-in-an-ex-vivo-and-in-vitro-model
#2
Veronica Della Latta, Manuela Cabiati, Silvia Burchielli, Giada Frenzilli, Margherita Bernardeschi, Antonella Cecchettini, Federica Viglione, Maria-Aurora Morales, Silvia Del Ry
Pulmonary fibrosis (PF) is the most common and aggressive interstitial lung disease, characterized by a patchy development of fibrosis leading to progressive destruction of the normal lung architecture which is preceded by an inflammatory process. Gene expression studies are important to understand the development of PF but the accuracy and reproducibility of Real-Time PCR depend on appropriate normalization strategies. This study aimed to analyze the expression variability of eight commonly used reference genes during the initial inflammatory phase of bleomycin-induced PF in a mouse model and to verify whether the selected reference genes could be applied to an in-vitro model of BLM-treated primary murine lung fibroblasts...
May 16, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28524369/therapeutic-roles-of-telocytes-in-ova-induced-acute-asthma-in-mice
#3
Ling Ye, Dongli Song, Meiling Jin, Xiangdong Wang
Telocytes (TCs) newly discovered as the mesenchyme-derived interstitial cells were found to have supportive effects on mesenchymal stem cells (MSCs). The present study aimed at investigating effects of TCs or TCs gathered with MSCs on experimental airway inflammation and hyper-responsiveness. The TCs were isolated from the lung tissue of the female BALB/c mice. The ovalbumin (OVA)-induced asthma model was established. TCs (1 × 10(6) /2 × 10(6) ) and/or MSCs (1 × 10(6) ) were injected through mice tail vein for consecutive three days before OVA excited the mice...
May 19, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28523981/classification-of-lunge-biomechanics-with-multiple-and-individual-inertial-measurement-units
#4
Martin A O'Reilly, Darragh F Whelan, Tomas E Ward, Eamonn Delahunt, Brian Caulfield
Lunges are a common, compound lower limb resistance exercise. If completed with aberrant technique, the increased stress on the joints used may increase risk of injury. This study sought to first investigate the ability of inertial measurement units (IMUs), when used in isolation and combination, to (a) classify acceptable and aberrant lunge technique (b) classify exact deviations in lunge technique. We then sought to investigate the most important features and establish the minimum number of top-ranked features and decision trees that are needed to maintain maximal system classification efficacy...
May 19, 2017: Sports Biomechanics
https://www.readbyqxmd.com/read/28523921/combination-of-h120-and-1-96-avian-infectious-bronchitis-virus-vaccine-strains-protect-chickens-against-challenge-with-is-1494-06-variant-2-like-infectious-bronchitis-virus
#5
M Habibi, V Karimi, A G Langeroudi, S A Ghafouri, M Hashemzadeh, R K Farahani, H Maghsoudloo, H Abdollahi, P Seifouri
Avian infectious bronchitis (IB) is a worldwide chicken disease, caused by avian infectious bronchitis virus (IBV) which infects all commercial poultry lines. The present study was done to evaluate protection caused by two different serotype vaccines (Massachusetts and 793/B) in order to evaluate protection against challenge with IS/1494/06-like virus (variant 2-like virus), which is prevalent in the Middle East. SPF chickens were divided into four groups (n = 20). First and second group as negative control group and non-vaccinated-challenged group received no vaccine...
2017: Acta Virologica
https://www.readbyqxmd.com/read/28523388/detection-and-genetic-characterization-of-pasteurella-multocida-from-alpaca-vicugna-pacos-pneumonia-cases
#6
Rocío Rímac, Luis Luna, Raquel Hurtado, Raúl Rosadio, Lenin Maturrano
Pasteurella multocida is a common constituent of upper respiratory tract microbiota but is frequently isolated of alpaca lung tissues from pulmonary infections. Despite its importance, very little is known about this bacteria at molecular level. In order to characterize P. multocida isolates, 24 isolates recovered from 46 mortal acute cases in young alpacas with suspected pneumonia were analyzed, using biochemical and molecular tests for capsule and LPS typing, virulence factors detection, and ERIC-PCR genetic diversity analysis...
May 18, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28522873/recombinant-bcg-expressing-ltak63-adjuvant-induces-superior-protection-against-mycobacterium-tuberculosis
#7
Ivan P Nascimento, Dunia Rodriguez, Carina C Santos, Eduardo P Amaral, Henrique K Rofatto, Ana P Junqueira-Kipnis, Eduardo D C Gonçalves, Maria R D'Império-Lima, Mario H Hirata, Celio L Silva, Nathalie Winter, Brigitte Gicquel, Kingston H G Mills, Mariagrazia Pizza, Rino Rappuoli, Luciana C C Leite
In order to develop an improved BCG vaccine against tuberculosis we have taken advantage of the adjuvant properties of a non-toxic derivative of Escherichia coli heat labile enterotoxin (LT), LTAK63. We have constructed rBCG strains expressing LTAK63 at different expression levels. Mice immunized with BCG expressing low levels of LTAK63 (rBCG-LTAK63lo) showed higher Th1 cytokines and IL-17 in the lungs, and when challenged intratracheally with Mycobacterium tuberculosis displayed a 2.0-3.0 log reduction in CFU as compared to wild type BCG...
May 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28522728/draft-genome-sequence-of-mycobacterium-abscessus-bamboo
#8
Michelle Yee, David Klinzing, Jun-Rong Wei, Martin Gengenbacher, Eric J Rubin, Thomas Dick
Mycobacterium abscessus, an intrinsically multidrug-resistant pathogen, causes chronic incurable lung disease. New drugs for this emerging pathogen represent an urgent unmet medical need. Here, we report a draft genome sequence of M. abscessus Bamboo, a clinical isolate used as a screening strain for drug discovery.
May 18, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28522561/immediate-and-delayed-potentiating-effects-of-tumor-necrosis-factor-alpha-on-trpv1-sensitivity-of-rat-vagal-pulmonary-sensory-neurons
#9
Chun-Chun Hsu, You Shuei Lin, Ruei-Lung Lin, Lu-Yuan Lee
We studied acute effects of tumor necrosis factor-alpha (TNFα) on the sensitivity of isolated rat vagal pulmonary sensory neurons. Our results showed: 1) A brief pretreatment with a low dose of TNFα (1.44 nM, 9 min) enhanced the sensitivity of transient receptor potential vanilloid type 1 (TRPV1) receptors in these neurons in two distinct phases: the inward current evoked by capsaicin was amplified (∆=247%) immediately following the TNFα pretreatment, which gradually declined toward control and then increased again reaching another peak (∆=384%) after 60-90 min...
May 18, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28522112/diffuse-parenchymal-lung-disease-on-chest-radiographs-and-ct
#10
Theodore J Dubinsky, Hardik Shah, Rachelle Sonneborn, Daniel S Hippe
BACKGROUND: We prospectively identified B-lines in patients undergoing ultrasound examinations following liver transplants who also had a chest x-ray (CXR) and/or chest CT scan on the same day to determine if an association between the presence of B-lines s from the thorax on ultrasound images correlates with the presence of pulmonary abnormalities on chest radiographs. MATERIALS AND METHODS: Following IRB approval, patients undergoing routine postoperative liver transplant ultrasound examinations and a CXR and/or CT scan on the same day between 1/1/2015 through 7/1/2016 were prospectively identified...
May 15, 2017: Chest
https://www.readbyqxmd.com/read/28521570/two-novel-compounds-from-the-root-bark-of-morus-alba-l
#11
Ming Li, Xuewei Wu, Xiaoning Wang, Tao Shen, Dongmei Ren
Chemical investigation of the root bark of Morus alba led to the isolation of a new flavone, dioxycudraflavone A (1) and a new 2-arylbenzofuran, 5-hydroxyethyl moracin M (2), together with seven known compounds namely sanggenon V (3), morusin (4), morusignin L (5), licoflavone C (6), moracin C (7), alfafuran (8) and mulberrofuran G (9). The structure elucidation of these compounds was based on analyses of spectroscopic data including 1D, 2D NMR and HR-ESI-MS. All compounds were evaluated for the α-glucosidase inhibitory and cytotoxic activities...
May 19, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28521225/recovery-of-gold-from-industrial-wastewater-by-extracellular-proteins-obtained-from-a-thermophilic-bacterium-tepidimonas-fonticaldi-at-a2
#12
Yin-Lung Han, Jen-Hao Wu, Chieh-Lun Cheng, Dillirani Nagarajan, Ching-Ray Lee, Yi-Heng Li, Yung-Chung Lo, Jo-Shu Chang
Biosorption has emerged as a promising alternative approach for treating wastewater with dilute metal contents in a green and cost effective way. In this study, extracellular proteins of an isolated thermophilic bacterium (Tepidimonas fonticaldi AT-A2) were used as biosorbent to recover precious metal (i.e., Au) from wastewater. The Au (III) adsorption capacity on the T. fonticaldi AT-A2 proteins was the highest when the pH was set at about 4.0-5.0. The adsorption capacity increased with increasing temperature from 15 to 70°C...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28518083/bronchoalveolar-lavage-of-murine-lungs-to-analyze-inflammatory-cell-infiltration
#13
Lien Van Hoecke, Emma Richelle Job, Xavier Saelens, Kenny Roose
Bronchoalveolar Lavage (BAL) is an experimental procedure that is used to examine the cellular and acellular content of the lung lumen ex vivo to gain insight into an ongoing disease state. Here, a simple and efficient method is described to perform BAL on murine lungs without the need of special tools or equipment. BAL fluid is isolated by inserting a catheter in the trachea of terminally anesthetized mice, through which a saline solution is instilled into the bronchioles. The instilled fluid is gently retracted to maximize BAL fluid retrieval and to minimize shearing forces...
May 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28516066/the-alpha-tocopherol-form-of-vitamin-e-boosts-elastase-activity-of-human-pmns-and-their-ability-to-kill-streptococcus-pneumoniae
#14
Elsa N Bou Ghanem, James N Lee, Basma H Joma, Simin N Meydani, John M Leong, Alexander Panda
Despite the availability of vaccines, Streptococcus pneumoniae remains a leading cause of life-threatening infections, such as pneumonia, bacteremia and meningitis. Polymorphonuclear leukocytes (PMNs) are a key determinant of disease course, because optimal host defense requires an initial robust pulmonary PMN response to control bacterial numbers followed by modulation of this response later in infection. The elderly, who manifest a general decline in immune function and higher basal levels of inflammation, are at increased risk of developing pneumococcal pneumonia...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28515374/polyphyllin-i-overcomes-emt-associated%C3%A2-resistance%C3%A2-to-erlotinib-in-lung-cancer-cells-via-il-6-stat3-pathway-inhibition
#15
Wei Lou, Yan Chen, Ke-Ying Zhu, Huizi Deng, Tianhao Wu, Jun Wang
Acquired resistance to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) is the most important limiting factor for treatment efficiency in EGFR-mutant non-small cell lung cancer (NSCLC). Much work has linked the epithelial-mesenchymal transition (EMT) to the emergence of drug resistance, consequently, ongoing research has been focused on exploring the therapeutic options to reverse EMT for delaying or preventing drug resistance. Polyphyllin I (PPI) is a natural compound isolated from Paris polyphylla rhizomes and displayed anti-cancer properties...
May 18, 2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28515041/multidisciplinary-evaluation-of-interstitial-lung-diseases-current-insights-number-1-in-the-series-radiology-edited-by-nicola-sverzellati-and-sujal-desai
#16
Simon L F Walsh
Multidisciplinary team (MDT) diagnosis is regarded as the diagnostic reference standard for interstitial lung disease (ILD). Several studies have reported that MDT diagnosis is associated with higher levels of diagnostic confidence and better interobserver agreement when compared to the individual components of the MDT in isolation. Although this recommendation is widely accepted, no guideline statement specifies what constitutes an MDT meeting and how its participants should govern it. Furthermore, the precise role of an MDT meeting in the setting of ILD may vary from one group to another...
June 30, 2017: European Respiratory Review: An Official Journal of the European Respiratory Society
https://www.readbyqxmd.com/read/28512704/pseudomonas-aeruginosa-inhibits-the-growth-of-scedosporium-and-lomentospora-in-vitro
#17
Sharon C-A Chen, Shilpa Patel, Wieland Meyer, Belinda Chapman, Hong Yu, Karen Byth, Peter G Middleton, Helena Nevalainen, Tania C Sorrell
In vitro bacterial-fungal interaction studies in cystic fibrosis (CF) have mainly focused on interactions between bacteria and Candida. Here we investigated the effect of Pseudomonas aeruginosa on the growth of Scedosporium/Lomentospora spp. Standard suspensions of P. aeruginosa (16 non-mucoid and nine mucoid isolates) were dropped onto paper disks, placed on lawns of Lomentospora prolificans (formerly Scedosporium prolificans) strain WM 14.140 or Scedosporium aurantiacum strain WM 11.78 on solid agar. The median inhibitory activity (mIz) was calculated for each fungal-bacterial combination...
May 16, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28509856/first-report-of-a-fatal-case-associated-with-ev-d68-infection-in-hong-kong-and-emergence-of-an-interclade-recombinant-in-china-revealed-by-genome-analysis
#18
Cyril C Y Yip, Janice Y C Lo, Siddharth Sridhar, David C Lung, Shik Luk, Kwok-Hung Chan, Jasper F W Chan, Vincent C C Cheng, Patrick C Y Woo, Kwok-Yung Yuen, Susanna K P Lau
A fatal case associated with enterovirus D68 (EV-D68) infection affecting a 10-year-old boy was reported in Hong Kong in 2014. To examine if a new strain has emerged in Hong Kong, we sequenced the partial genome of the EV-D68 strain identified from the fatal case and the complete VP1, and partial 5'UTR and 2C sequences of nine additional EV-D68 strains isolated from patients in Hong Kong. Sequence analysis indicated that a cluster of strains including the previously recognized A2 strains should belong to a separate clade, clade D, which is further divided into subclades D1 and D2...
May 16, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28508992/palivizumab-prophylaxis-in-infants-with-cystic-fibrosis-does-not-delay-first-isolation-of-pseudomonas-aeruginosa-or-staphylococcus-aureus
#19
Clélia Buchs, Marie-Laure Dalphin, Stéphane Sanchez, Marie Perceval, Laurianne Coutier, Catherine Mainguy, Behrouz Kassaï-Koupaï, Philippe Reix
Respiratory syncytial virus (RSV) infections may worsen cystic fibrosis (CF) lung disease and favor Pseudomonas aeruginosa (Pa) or Staphylococcus aureus (Sa) acquisition, which is of particular importance in the youngest patients. We aimed to determine the effectiveness of PVZ on microbiological outcomes in young children with CF. We conducted a retrospective case-control study to compare these outcomes in children who systematically received PVZ (PVZ+; n = 40) or not (PVZ-; n = 140). One case was matched with at least three same-gender controls born the same year and month...
May 16, 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/28508743/mycobacterium-kansasii-isolated-from-tuberculinpositive-rhesus-macaques-macaca-mulatta-in-the-absence-of-disease
#20
Steven T Shipley, David K Johnson, Morteza Roodgar, David Glenn Smith, Charles A Montgomery, Steven M Lloyd, James A Higgins, Edwin H Kriel, Hilton J Klein, William P Porter, Jerome B Nazareno, Paul W Houghton, Aruna Panda, Louis J DeTolla
Mycobacterial infections are of primary health concern in NHP colonies in biomedical research. NHP are constantly monitoredand screened for Mycobacterium spp. We report 6 Chinese-origin rhesus macaques infected with Mycobacterium kansasii thatexhibited positive tuberculin skin tests in the absence of disease. Two of these macaques were being used for research purposes;the remaining 4 macaques were residing at the contract quarantine company. Histopathology and acid-fast staining of fixed tissues from all macaques showed that all were free of disease...
May 15, 2017: Comparative Medicine
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