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https://www.readbyqxmd.com/read/28331417/%C3%AE-tocotrienol-inhibits-tgf-%C3%AE-1-induced-contractile-phenotype-expression-of-human-airway-smooth-muscle-cells
#1
Takehito Fukushima, Akira Yamasaki, Tomoya Harada, Hiroki Chikumi, Masanari Watanabe, Ryota Okazaki, Miki Takata, Yasuyuki Hasegawa, Jun Kurai, Masaaki Yanai, Akihiro Yamamoto, Yuriko Sueda, Andrew J Halayko, Eiji Shimizu
BACKGROUND: Tocotrienols, members of the vitamin E family, exist in four different isoforms (α, β, γ and δ tocotrienol) that have can be protective against brain damage, as well as having anticancer effects in vivo and in vitro. We have shown that γ-tocotrienol inhibits human airway smooth muscle cell proliferation and migration induced by platelet-derived growth factor (PDGF)-BB by suppressing RhoA activation. In this study, we tested whether γ-tocotrienol modulates transforming growth factor (TGF) -β-induced induction of human airway smooth muscle (ASM) into a contractile phenotype and concomitant synthesis of extracellular matrix proteins...
March 2017: Yonago Acta Medica
https://www.readbyqxmd.com/read/28331074/osteopontin-inhibits-osteoblast-responsiveness-through-the-downregulation-of-focal-adhesion-kinase-mediated-by-the-induction-of-low-molecular-weight-protein-tyrosine-phosphatase
#2
Joji Kusuyama, Kenjiro Bandow, Tomokazu Ohnishi, Mitsuhiro Hisadome, Kaori Shima, Ichiro Semba, Tetsuya Matsuguchi
Osteopontin (OPN) is an osteogenic marker protein. Osteoblast functions are affected by inflammatory cytokines and pathological conditions. OPN is highly expressed in bone legions such as rheumatoid arthritis. However, local regulatory effects of OPN on osteoblasts remain ambiguous. Here, we examined how OPN influences osteoblast responses to mechanical stress and growth factors. Expression of NO synthase 1 (Nos1) and Nos2 was increased by low intensity pulsed ultrasound (LIPUS) in MC3T3-E1 cells and primary osteoblasts...
March 22, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28330949/expression-analysis-of-platelet-derived-growth-factor-receptor-alpha-and-its-ligands-in-the-developing-mouse-lung
#3
Leonor Gouveia, Christer Betsholtz, Johanna Andrae
Activation of the platelet-derived growth factor receptor-α (PDGFRα) signaling pathway is critically important during lung alveogenesis, the process in lung development during which alveoli are formed from the terminal alveolar sacs. Several studies have aimed to characterize the expression patterns of PDGFRα and its two ligands (PDGF-A and -C) in the lung, but published analyses have been limited to embryonic and/or perinatal time points, and no attempts have been made to characterize both receptor and ligand expression simultaneously...
March 2017: Physiological Reports
https://www.readbyqxmd.com/read/28327812/platelet-derived-growth-factor-a-mrna-in-platelets-is-associated-with-the-degree-of-hepatic-fibrosis-in-chronic-hepatitis-c
#4
Aline Aki Tanikawa, Rejane Maria Tommasini Grotto, Giovanni Faria Silva, Adriana Camargo Ferrasi, Valéria Cristina Rodrigues Sarnighausen, Maria Inês de Moura Campos Pardini
INTRODUCTION: Transforming growth factor beta 1 (TGFB1) and platelet-derived growth factor (PDGF) are the main cytokines related to hepatic fibrogenesis. METHODS: RNA isolated from the platelets and hepatic tissue of 43 HCV carriers was used for quantitative polymerase chain reaction to determine TGFB1, PDGFA, and PDGFB RNA expression. RESULTS: The mRNA expression of PDGFA in platelets was significantly lower in the group with advanced fibrosis than in the group with early-stage fibrosis...
January 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/28327358/a-cell-autonomous-positive-signaling-circuit-associated-with-the-pdgf-no-id4-regulatory-axis-in-glioblastoma-cells
#5
Kiyoung Eun, Hye-Min Jeon, Sung-Ok Kim, Sang-Hun Choi, Seon Yong Lee, Xiong Jin, Sung-Chan Kim, Hyunggee Kim
Most cancer-related signaling pathways sustain their active or inactive status via genetic mutations or various regulatory mechanisms. Previously, we demonstrated that platelet-derived growth factor (PDGF) activates Notch signaling through nitric oxide (NO)-signaling-driven activation of inhibitor of differentiation 4 (ID4) in glioblastoma (GBM) stem cells (GSCs) and endothelial cells in the vascular niche of GBM, leading to maintenance of GSC traits and GBM progression. Here, we determined that the PDGF-NO-ID4-signaling axis is constantly activated through a positive regulatory circuit...
March 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28327149/release-kinetics-and-mitogenic-capacity-of-collagen-barrier-membranes-supplemented-with-secretome-of-activated-platelets-the-in-vitro-response-of-fibroblasts-of-the-periodontal-ligament-and-the-gingiva
#6
Eva-Maria Mozgan, Michael Edelmayer, Klara Janjić, Manuela Pensch, Michael B Fischer, Andreas Moritz, Hermann Agis
BACKGROUND: Platelet preparations can stimulate the healing process and have mitogenic properties. We hypothesized that collagen barrier membranes (CBM), clinically used in guided bone regeneration and guided tissue regeneration, can serve as carriers for platelet secretome. METHODS: Secretome was generated from washed platelets and unwashed platelets (washed/unwashed PSEC) and lyophilized onto CBM. Overall appearance of CBM was evaluated by scanning electron microscopy...
March 21, 2017: BMC Oral Health
https://www.readbyqxmd.com/read/28324485/maintenance-of-human-embryonic-stem-cells-by-sphingosine-1-phosphate-and-platelet-derived-growth-factor
#7
Raymond C B Wong, Martin F Pera, Alice Pébay
Human embryonic stem cells (hESCs) have historically been cultivated on feeder layers of primary mouse embryonic fibroblasts (MEF) in a medium supplemented with fetal calf serum (FCS). However, serum contains a wide variety of biologically active compounds that might adversely affect hESC growth and differentiation. Thus, cultivation of stem cells in FCS complicates experimental approaches to define the intracellular mechanisms required for hESC maintenance. This chapter describes the serum-free maintenance of hESCs in culture by addition of sphingosine-1-phosphate (S1P) and platelet-derived growth factor (PDGF)...
March 22, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28323386/the-association-of-maternal-lymphatic-markers-and-critical-congenital-heart-defects-in-the-fetus-a-population-based-case-control-study
#8
Martina A Steurer, Mary E Norton, Rebecca J Baer, Gary M Shaw, Sheila Keating, Anita J Moon-Grady, Christina D Chambers, Laura L Jelliffe-Pawlowski
The objective ot this study was to investigate whether lymphatic markers measured in women during the second trimester are associated with critical congenital heart defects (CCHDs) in offspring. This is a retrospective cohort study of pregnant women who participated in the California Prenatal Screening Program. CCHD data in the offspring was captured by linking birth certificate data with hospital patient discharge records. Second trimester samples were assayed for vascular endothelial growth factor (VEGF), platelet derived growth factor (PDGF) AA/BB, and PDGF AB...
March 21, 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/28322737/the-tead-family-transcription-factor-scalloped-regulates-blood-progenitor-maintenance-and-proliferation-in-drosophila-through-pdgf-vegfr-receptor-pvr-signaling
#9
Gabriel B Ferguson, Julian A Martinez-Agosto
The Drosophila lymph gland is a well-characterized hematopoietic organ in which a population of multipotent stem-like progenitors is maintained by a combination of signals from different cellular populations within the organ. The lymph gland serves as an ideal model both for the interrogation of signaling mechanisms involved in progenitor maintenance as well as a tool for the identification of novel regulatory mechanisms in the highly conserved process of hematopoiesis. Here, we demonstrate a requirement for the TEAD transcription factor Scalloped in the maintenance and proliferation of hematopoietic progenitors...
March 18, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28320405/urinary-collagen-degradation-products-as-early-markers-of-progressive-renal-fibrosis
#10
Ryanne S Hijmans, Daniel Guldager Kring Rasmussen, Saleh Yazdani, Gerjan Navis, Harry van Goor, Morten Asser Karsdal, Federica Genovese, Jacob van den Born
BACKGROUND: Renal fibrogenesis is associated with increased ECM remodeling and release of collagen fragments in urine in progressive renal disease. We investigated the diagnostic value of urinary collagen degradation products in a proteinuria-driven fibrosis rat model with and without anti-fibrotic S1P-receptor modulator FTY720 treatment. METHODS: Proteinuria was induced in male Wistar rats by Adriamycin (ADR) injection (n = 16). Healthy rats served as controls (n = 12)...
March 20, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28318371/p-selectin-endocan-and-some-adhesion-molecules-in-obese-children-and-adolescents-with-non-alcoholic-fatty-liver-disease
#11
Ala Ustyol, Esra Aycan Ustyol, Figen Gurdol, Funda Kokali, Seldag Bekpınar
There is increasing evidence for a direct relationship between the vascular system and non-alcoholic fatty liver disease (NAFLD). The aim of this study was to investigate endocan and adhesion molecules such as P-selectin derived from the endothelium and platelets in obese children and adolescents with NAFLD. One hundred obese patients and 40 lean controls were enrolled. The obese subjects were divided into two subgroups based on the presence or absence of fatty liver. Blood samples were assayed for endocan, P-selectin, platelet-derived growth factor (PDGF), intercellular cell adhesion molecule (ICAM)-1, and vascular cell adhesion molecule (VCAM)-1...
May 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/28315522/human-cardiac-fibroblasts-adaptive-responses-to-controlled-combined-mechanical-strain-and-oxygen-changes-in-vitro
#12
Giovanni Stefano Ugolini, Andrea Pavesi, Marco Rasponi, Gianfranco Beniamino Fiore, Roger Kamm, Monica Soncini
Upon cardiac pathological conditions such as ischemia, microenvironmental changes instruct a series of cellular responses that trigger cardiac fibroblasts-mediated tissue adaptation and inflammation. A comprehensive model of how early environmental changes may induce cardiac fibroblasts (CF) pathological responses is far from being elucidated, partly due to the lack of approaches involving complex and simultaneous environmental stimulation. Here, we provide a first analysis of human primary CF behavior by means of a multi-stimulus microdevice for combined application of cyclic mechanical strain and controlled oxygen tension...
March 18, 2017: ELife
https://www.readbyqxmd.com/read/28314909/localization-of-dynamin-related-protein-1-and-its-potential-role-in-lamellipodia-formation
#13
Youhwa Jo, Hyo Min Cho, Woong Sun, Jae Ryun Ryu
Dynamin-related protein1 (Drp1) plays an essential role in mitochondrial fission: Cytosolic Drp1 is translocated to the mitochondria upon stimulus, and oligomerized Drp1 constricts mitochondria by aid of actin filaments. Drp1 completes the fission process with GTP hydrolysis by its own GTPase activity. The importance of actin filament and its interaction with Drp1 in the mitochondrial fission process have been demonstrated. In this study, we found that Drp1 is enriched in the actin-rich leading edge of lamellipodia of mouse embryonic fibroblasts (MEFs) wherein mitochondria or peroxisomes are absent...
March 17, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28314864/systematic-model-of-peripheral-inflammation-after-subarachnoid-hemorrhage
#14
Jude P J Savarraj, Kaushik Parsha, Georgene W Hergenroeder, Liang Zhu, Suhas S Bajgur, Sungho Ahn, Kiwon Lee, Tiffany Chang, Dong H Kim, Yin Liu, H Alex Choi
OBJECTIVE: To investigate inflammatory processes after aneurysmal subarachnoid hemorrhage (aSAH) with network models. METHODS: This is a retrospective observational study of serum samples from 45 participants with aSAH analyzed at multiple predetermined time points: <24 hours, 24 to 48 hours, 3 to 5 days, and 6 to 8 days after aSAH. Concentrations of cytokines were measured with a 41-plex human immunoassay kit, and the Pearson correlation coefficients between all possible cytokine pairs were computed...
March 17, 2017: Neurology
https://www.readbyqxmd.com/read/28302707/neuropeptide-y3-36-incorporated-into-pvax-nanoparticle-improves-functional-blood-flow-in-a-murine-model-of-hind-limb-ischemia
#15
Derek Eshun, Rabya Saraf, Soochan Bae, Jelliffe Jeganathan, Feroze Mahmood, Serkan Dilmen, Qingen Ke, Dongwon Lee, Peter M Kang, Robina Matyal
We generated a novel nanoparticle called PVAX, which has intrinsic anti-apoptotic and anti-inflammatory properties. This nanoparticle was loaded with Neuropeptide Y3-36, an angiogenic neuro-hormone that plays a central role in angiogenesis. Subsequently, we investigated whether PVAX-NPY3-36 could act as a therapeutic agent and induce angiogenesis and vascular remodeling in a murine model of hind limb ischemia. Adult C57BL/J6 mice (n=40) were assigned to treatment groups: Control, Ischemia PBS, Ischemia PVAX, Ischemia NPY3-36, and Ischemia PVAX-NPY3-36 Ischemia was induced by ligation of the femoral artery in all groups except Control and given relevant treatments (PBS, PVAX, NPY3-36, and PVAX-NPY3-36)...
March 16, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28300261/quantifying-3d-chemotaxis-in-microfluidic-based-chips-with-step-gradients-of-collagen-hydrogel-concentrations
#16
C Del Amo, C Borau, N Movilla, Jesús Asín, J M García-Aznar
Cell migration is an essential process involved in crucial stages of tissue formation, regeneration or immune function as well as in pathological processes including tumor development or metastasis. During the last few years, the effect of gradients of soluble molecules on cell migration has been widely studied, and complex systems have been used to analyze cell behavior under simultaneous mechano-chemical stimuli. Most of these chemotactic assays have, however, focused on specific substrates in 2D. The aim of the present work is to develop a novel microfluidic-based chip that allows the long-term chemoattractant effect of growth factors (GFs) on 3D cell migration to be studied, while also providing the possibility to analyze the influence of the interface generated between different adjacent hydrogels...
March 16, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/28297834/-effect-of-inhibiting-perivascular-adhesion-on-intimal-hyperplasia-of-the-vein-grafts-in-rabbits
#17
M K Guo, J X Zhu, C M Han, T Bao, D Yang, P Yu, Y F Duan, F L Fan, H G Zhang, H J Tian
Objective: To investigate the effect of preventing perivascular adhesion with topical application of sodium hyaluronate on intimal hyperplasia of the vein grafts in rabbits. Methods: Twenty-four male New Zealand white rabbits, aged 5 months, were randomly divided into 2 groups: Group A and B (n=12 rabbits per group). Artery defect model was established by cutting about 1 cm artery from the middle part of the dissociated left common carotid artery. A section about 3 cm was cut from the right external jugular vein, and the harvested vein was inverted and end-to-end anastomosed to the artery defect...
March 7, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28296053/vasoactive-effect-of-fibronectin-derived-epiviosamine-1-and-related-peptides-in-quiescent-and-stress-models
#18
Mary D Frame, Fubao Lin, Anthony M Dewar, Richard Af Clark
OBJECTIVE: Following thermal burn injury, plasma fibronectin degrades within the interstitium; one possible product is epiviosamine-1 (EVA-1, PSHISKYILRWRPK) found within the first type III repeat of plasma fibronectin (FNIII1 ). EVA-1 ameliorates thermal burn injury progression, and binds to and enhances platelet-derived growth factor-BB (PDGF-BB) in promoting cell metabolism, growth and survival; shorter related peptides lose these abilities. Here we study the effect of EVA-1 and shorter peptides for their vasoactivity under quiescent and stress conditions...
March 15, 2017: Microcirculation: the Official Journal of the Microcirculatory Society, Inc
https://www.readbyqxmd.com/read/28294563/a-new-material-for-tissue-engineered-vagina-reconstruction-acellular-porcine-vagina-matrix
#19
Jing-Kun Zhang, Run-Xuan Du, Lin Zhang, Ya-Nan Li, Ming-le Zhang, Shuo Zhao, Xiang-Hua Huang, Yan-Fang Xu
Acellular matrix materials have been widely used to repair various tissues and organs. According to the plastic principle, when a part of the body is lost, it should be replaced with a similar material. Therefore, the use of a homologous organ-specific acellular vaginal tissue in vagina reconstruction repair surgery may show good results. However, the acellular vagina matrix (AVM) form large vertebrates is difficult to isolate. In this study, we described a multi-step method to prepare porcine AVM and evaluated the efficacy of acellularization...
March 10, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28290607/homemade-device-induced-negative-pressure-promotes-wound-healing-more-efficiently-than-vsd-induced-positive-pressure-by-regulating-inflammation-proliferation-and-remodeling
#20
Jinyan Liu, Feng Hu, Jintian Tang, Shijie Tang, Kun Xia, Song Wu, Chaoqi Yin, Shaohua Wang, Quanyong He, Huiqing Xie, Jianda Zhou
Vacuum sealing drainage (VSD) is an effective technique used to promote wound healing. However, recent studies have shown that it exerts positive pressure (PP) rather than negative pressure (NP) on skin. In this study, we created a homemade device that could maintain NP on the wound, and compared the therapeutic effects of VSD-induced PP to those of our homemade device which induced NP on wound healing. The NP induced by our device required less time for wound healing and decreased the wound area more efficiently than the PP induced by VSD...
March 13, 2017: International Journal of Molecular Medicine
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