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https://www.readbyqxmd.com/read/28723877/human-spinal-bone-dust-as-a-potential-local-autograft-in-vitro-potent-anabolic-effect-on-human-osteoblasts
#1
Ryan Gao, Matthew Street, Mei Lin Tay, Karen E Callon, Dorit Naot, Alistair Lock, Jacob T Munro, Jillian Cornish, John Ferguson, David Musson
STUDY DESIGN: In Vitro Study. OBJECTIVE: To evaluate the effect that factors released from human posterior spinal bone dust have on primary human osteoblast growth and maturation. SUMMARY OF BACKGROUND DATA: Bone dust, created during spinal fusion surgeries has the potential to be used as an autologous bone graft by providing a source of viable autologous osteoblasts and mesenchymal stem cells with osteogenic potential. To date, no information is available on whether bone dust also provides a source of anabolic factors with the potential to enhance osteoblast proliferation and maturation, which would enhance its therapeutic potential...
July 18, 2017: Spine
https://www.readbyqxmd.com/read/28719662/the-galnac-t-activation-pathway-gala-is-not-a-general-mechanism-for-regulating-mucin-type-o-glycosylation
#2
Gaetan G Herbomel, Raul E Rojas, Duy T Tran, Monica Ajinkya, Lauren Beck, Lawrence A Tabak
Mucin-type O-glycosylation is initiated by the UDP-GalNAc polypeptide:N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. Their activity results in the GalNAc α1-O-Thr/Ser structure, termed the Tn antigen, which is further decorated with additional sugars. In neoplastic cells, the Tn antigen is often overexpressed. Because O-glycosylation is controlled by the activity of GalNAc-Ts, their regulation is of great interest. Previous reports suggest that growth factors, EGF or PDGF, induce Golgi complex-to-endoplasmic reticulum (ER) relocation of both GalNAc-Ts and Tn antigen in HeLa cells, offering a mechanism for Tn antigen overexpression termed "GALA"...
2017: PloS One
https://www.readbyqxmd.com/read/28717129/3d-printed-scaffolds-of-calcium-silicate-doped-%C3%AE-tcp-synergize-with-co-cultured-endothelial-and-stromal-cells-to-promote-vascularization-and-bone-formation
#3
Yuan Deng, Chuan Jiang, Cuidi Li, Tao Li, Mingzheng Peng, Jinwu Wang, Kerong Dai
Synthetic bone scaffolds have potential application in repairing large bone defects, however, inefficient vascularization after implantation remains the major issue of graft failure. Herein, porous β-tricalcium phosphate (β-TCP) scaffolds with calcium silicate (CS) were 3D printed, and pre-seeded with co-cultured human umbilical cord vein endothelial cells (HUVECs) and human bone marrow stromal cells (hBMSCs) to construct tissue engineering scaffolds with accelerated vascularization and better bone formation...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28714058/in-vitro-and-in-vivo-effects-of-pdgf-bb-delivery-strategies-on-tendon-healing-a-review
#4
O Evrova, J Buschmann
To promote and support tendon healing, one viable strategy is the use or administration of growth factors at the wound/rupture site. Platelet derived growth factor-BB (PDGF-BB), together with other growth factors, is secreted by platelets after injury. PDGF-BB promotes mitogenesis and angiogenesis, which could accelerate tendon healing. Therefore, in vitro studies with PDGF-BB have been performed to determine its effect on tenocytes and tenoblasts. Moreover, accurate and sophisticated drug delivery devices, aiming for a sustained release of PDGF-BB, have been developed, either by using heparin-binding and fibrin-based matrices or different electrospinning techniques...
July 17, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28714000/circulating-levels-of-angiogenesis-related-growth-factors-in-breast-cancer-a-study-to-profile-proteins-responsible-for-tubule-formation
#5
Gemma A Barron, Marie Goua, Klaus W J Wahle, Giovanna Bermano
The present study exploited a versatile in vitro endothelial cell/fibroblast co-culture cell system to investigate the association between angiogenesis and breast cancer by comparing the capacity of plasma from women with breast cancer and age-matched controls, to influence tubule formation and modulate angiogenesis in vitro, and to identify plasma circulating factors which might be responsible. Plasma from women with breast cancer (n=8) (added on day 7 after co-culture establishment) significantly increased tubule formation by 57% (P<0...
July 11, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28713961/adipose-differentiation%C3%A2-related-protein-knockdown-inhibits-vascular-smooth-muscle-cell-proliferation-and-migration-and-attenuates-neointima-formation
#6
Haomin Zhao, Tao Han, Xin Hong, Dajun Sun
Vascular smooth muscle cells (VSMCs) have an important role in atherosclerosis development. Evidence has demonstrated that adipose differentiation‑related protein (ADRP) is associated with foam cell formation and atherosclerosis progression. However, to the best of our knowledge, no previous studies have investigated the role of ADRP knockdown in platelet‑derived growth factor (PDGF)‑stimulated proliferation and migration of VSMCs in vitro. Furthermore, the effect of ADRP knockdown on neointima formation in vivo remains unclear...
July 15, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28710554/morphofunctional-changes-after-sleeve-gastrectomy-and-very-low-calorie-diet-in-an-animal-model-of-non-alcoholic-fatty-liver-disease
#7
Eider Talavera-Urquijo, Sarai Rodríguez-Navarro, Marc Beisani, Maria Teresa Salcedo-Allende, Aisha Chakkur, Marc Arús-Avilés, Manel Cremades, Salvador Augustin, María Martell, José M Balibrea
BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver disease and is found in 70% of obese people. The evidence available to date suggests that bariatric surgery could be an effective treatment by reducing weight and also by improving metabolic complications in the long term. This work aimed to compare, in a diet-induced NAFLD animal model, the effect of both sleeve gastrectomy (SG) and very-low calorie diet (VLCD). METHODS: Thirty-five Wistar rats were divided into control rats (n = 7) and obese rats fed a high-fat diet (HFD)...
July 15, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/28702959/treatment-of-skin-defects-with-growth-factors-and-biodegradable-collagen-carrier-histological-evaluation-in-animal-model
#8
G B Menchini Fabris, P F Nocini, F Gelpi, J Lotti, V Favero, G Zanotti, A Jurlaro, I Rosskopf, T Lotti, A Barone, G Castegnaro, D De Santis
The aim of the present study is to evaluate the effects on skin regeneration of a new collagen matrix (CM-10826) when used in different combination with or without growth factors, using skin regeneration without membrane as control. An area of 10x15 cm on rabbit back was shaved and three circular wounds on test side were covered with a differently soaked membrane. The first wound was soaked with Epidermal Growth Factor (EGF, 26mg/130mL) (Test EGF), the second with Platelet-Derived Growth Factor (PDGF, 6mg/120mL) (Test PDGF) and the third with EGF (13mg/65mL) and PDGF (3mg/60mL) (Test EGF+PDGF)...
July 13, 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28701355/mir-4632-mediates-pdgfbb-induced-proliferation-and-anti-apoptosis-of-human-pulmonary-artery-smooth-muscle-cells-via-targeting-cjun
#9
Zhengjiang Qian, Yanjiao Li, Jidong Chen, Xiang Li, Deming Gou
MicroRNAs (miRNAs) can regulate proliferative status of pulmonary artery smooth muscle cells (PASMCs), which is a core factor modulating pulmonary vascular remodeling diseases, such as atherosclerosis and pulmonary arterial hypertension (PAH). Our previous work has shown that miR-4632, a rarely reported miRNA, is significantly downregulated in Platelet-Derived Growth Factor (PDGF)BB-stimulated human pulmonary artery smooth muscle cells (HPASMCs), yet its cell function and the underlying molecular mechanisms remain to be elucidated...
July 12, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28700916/a-reaction-diffusion-model-explains-amplification-of-the-plc-pkc-pathway-in-fibroblast-chemotaxis
#10
Krithika Mohan, Jamie L Nosbisch, Timothy C Elston, James E Bear, Jason M Haugh
During the proliferative phase of cutaneous wound healing, dermal fibroblasts are recruited into the clotted wound by a concentration gradient of platelet-derived growth factor (PDGF), together with other spatial cues. Despite the importance of this chemotactic process, the mechanisms controlling the directed migration of slow-moving mesenchymal cells such as fibroblasts are not well understood. Here, we develop and analyze a reaction-diffusion model of phospholipase C/protein kinase C (PKC) signaling, which was recently identified as a requisite PDGF-gradient-sensing pathway, with the goal of identifying mechanisms that can amplify its sensitivity in the shallow external gradients typical of chemotaxis experiments...
July 11, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28700345/platelet-derived-growth-factor-c-promotes-human-melanoma-aggressiveness-through-activation-of-neuropilin-1
#11
Federica Ruffini, Lauretta Levati, Grazia Graziani, Simona Caporali, Maria Grazia Atzori, Stefania D'Atri, Pedro M Lacal
Despite recent progress in advanced melanoma therapy, identification of signalling pathways involved in melanoma switch from proliferative to invasive states is still crucial to uncover new therapeutic targets for improving the outcome of metastatic disease. Neuropilin-1 (NRP-1), a co-receptor for vascular endothelial growth factor-A (VEGF-A) tyrosine kinase receptors (VEGFRs), has been suggested to play a relevant role in melanoma progression. NRP-1 can be activated by VEGF-A also in the absence of VEGFRs, triggering specific signal transduction pathways (e...
June 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28699690/microrna-let-7g-suppresses-pdgf-induced-conversion-of-vascular-smooth-muscle-cell-into-the-synthetic-phenotype
#12
Tzu-Ming Wang, Ku-Chung Chen, Po-Yuan Hsu, Hsiu-Fen Lin, Yung-Song Wang, Chien-Yuan Chen, Yi-Chu Liao, Suh-Hang H Juo
Platelet-derived growth factor (PDGF) can promote vascular smooth muscle cells (VSMCs) to switch from the quiescent contractile phenotype to synthetic phenotype, which contributes to atherosclerosis. We aimed to investigate the role of microRNA let-7g in phenotypic switching. Bioinformatics prediction was used to find let-7g target genes in the PDGF/mitogen-activated protein kinase kinase kinase 1 (MEKK1)/extracellular signal-regulated kinase (ERK)/Krüppel-like factor-4 (KLF4) signalling pathway that affects VSMC phenotypic switching...
July 12, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28698077/examination-of-in-vitro-and-in-vivo-biocompatibility-of-alginate-hyaluronic-acid-microbeads-as-a-promising-method-in-cell-delivery-for-kidney-regeneration
#13
Jhaleh Amirian, Tran Thi Tuong Van, Sang-Ho Bae, Hae-Il Jung, Hwan-Jun Choi, Hyun-Deuk Cho, Byong-Taek Lee
In this study, alginate (ALG) and alginate-hyaluronic acid (ALG-HA) injectable microbeads, with the purpose of delivering stem cells for tissue engineering, were prepared by a spraying method into a CaCl2 solution that shows high porosity for the exchange of nutrition and waste. In addition, the size distribution and surface morphology was investigated using optical and scanning electron microscopy, respectively. The chemical structural properties of the ALG-HA microbeads were examined by Fourier transform infrared spectroscopy...
July 8, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28694192/laminar-shear-stress-suppresses-vascular-smooth-muscle-cell-proliferation-through-nitric-oxide-ampk-pathway
#14
Sun Ae Kim, Jin Young Sung, Chang Hoon Woo, Hyoung Chul Choi
In healthy condition, vascular smooth muscle cells (VSMCs) are not directly exposed to shear stresses, because they are shielded by endothelial cell (EC) layer that lines blood vessels. After injury to EC layer caused by rupture of atherosclerotic lesions or invasive techniques such as angioplasty, VSMCs are directly exposed to blood flow which modulate molecular signaling and function. In endothelium, exposure to fluid shear stress has been reported to induce AMP-activated protein kinase (AMPK) phosphorylation and nitric oxide (NO) production...
July 7, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28693920/s100b-promotes-injury-induced-vascular-remodeling-through-modulating-smooth-muscle-phenotype
#15
Teng Cao, Lei Zhang, Ling-Ling Yao, Fei Zheng, Lu Wang, Jian-Ye Yang, Ling-Yun Guo, Xing-Yuan Li, Yu-Wen Yan, Ya-Mu Pan, Miao Jiang, Long Chen, Jun-Ming Tang, Shi-You Chen, Jia-NingWang
S100B is a biomarker of nervous system injury, but it is unknown if it is also involved in vascular injury. In the present study, we investigated S100B function in vascular remodeling following injury. Balloon injury in rat carotid artery progressively induced neointima formation while increasing S100B expression in both neointimal vascular smooth muscle (VSMC) and serum along with an induction of proliferating cell nuclear antigen (PCNA). Knockdown of S100B by its shRNA delivered by adenoviral transduction attenuated the PCNA expression and neointimal hyperplasia in vivo and suppressed PDGF-BB-induced VSMC proliferation and migration in vitro...
July 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28692941/fibroblast-growth-factor-21-ameliorates-high-glucose-induced-fibrogenesis-in-mesangial-cells-through-inhibiting-stat5-signaling-pathway
#16
Shuai Li, Xiaochen Guo, Teng Zhang, Nan Wang, Junyan Li, Pengfei Xu, Shengqi Zhang, Guiping Ren, Deshan Li
Fibroblast growth factor 21 (FGF21) is a member of the FGF family and acts as a potent regulator of glucose and lipid homeostasis, but its effect on renal fibrosis and the underlying mechanisms are totally unknown. The purpose of this study was designed to investigate whether FGF21 has effect on high glucose-induced fibrogenesis in human mesangial cells (HMCs) and the underlying mechanism. High glucose is well known to stimulate the expression of extracelluar matrix (ECM) in human mesangial cells. In humans, overexpression and deposition of ECM lead to renal fibrosis...
July 7, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28692698/nitric-oxide-attenuates-overexpression-of-gi%C3%AE-proteins-in-vascular-smooth-muscle-cells-from-shr-role-of-ros-and-ros-mediated-signaling
#17
Oli Sarkar, Yuan Li, Madhu B Anand-Srivastava
Vascular smooth muscle cells (VSMC) from spontaneously hypertensive rats (SHR) exhibit decreased levels of nitric oxide (NO) that may be responsible for the overexpression of Giα proteins that has been shown as a contributing factor for the pathogenesis of hypertension in SHR. The present study was undertaken to investigate if increasing the intracellular levels of NO by NO donor S-Nitroso-N-acetyl-DL-penicillamine (SNAP) could attenuate the enhanced expression of Giα proteins in VSMC from SHR and explore the underlying mechanisms responsible for this response...
2017: PloS One
https://www.readbyqxmd.com/read/28692113/analysing-the-bioactive-makeup-of-demineralised-dentine-matrix-on-bone-marrow-mesenchymal-stem-cells-for-enhanced-bone-repair
#18
S J Avery, L Sadaghiani, A J Sloan, R J Waddington
Dentine matrix has proposed roles for directing mineralised tissue repair in dentine and bone; however, the range of bioactive components in dentine and specific biological effects on bone-derived mesenchymal stem cells (MSCs) in humans are less well understood. The aims of this study were to further elucidate the biological response of MSCs to demineralised dentine matrix (DDM) in enhancing wound repair responses and ascertain key contributing components. Dentine was obtained from human teeth and DDM proteins solubilised with ethylenediaminetetraacetic acid (EDTA)...
July 10, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28691538/olaratumab-for-soft-tissue-sarcoma
#19
Diego Teyssonneau, Antoine Italiano
Soft tissue sarcomas (STS) are rare malignant tumors. Unfortunately, the first-line doxorubicin-based treatment has not been improved since the 1970s. Platelet-derived growth factor (PDGF) receptor alpha (PDGFR-α) and its ligands are co-expressed in many types of cancer, including sarcomas. They are involved in stimulating growth and regulating stromal-derived fibroblasts and angiogenesis. PDGFR-α and its ligand may play an important role in tumorigenesis and be a potential target in the treatment of sarcomas...
July 10, 2017: Expert Opinion on Biological Therapy
https://www.readbyqxmd.com/read/28684416/thioredoxin-reductase-1-and-nadph-directly-protect-protein-tyrosine-phosphatase-1b-from-inactivation-during-h2o2-exposure
#20
Markus Dagnell, Paul E Pace, Qing Cheng, Jeroen Frijhoff, Arne Östman, Elias S J Arnér, Mark B Hampton, Christine C Winterbourn
Regulation of growth factor signaling involves reversible inactivation of protein tyrosine phosphatases (PTPs) through the oxidation and reduction of their active site cysteine. However, there is limited mechanistic understanding of these redox events and their coordination in the presence of cellular antioxidant networks. Here, we investigated interactions between PTP1B and the peroxiredoxin 2 (Prx2)/thioredoxin 1 (Trx1)/thioredoxin reductase 1 (TrxR1) network. We found that Prx2 becomes oxidized in PDGF-treated fibroblasts, but only when TrxR1 has first been inhibited...
July 6, 2017: Journal of Biological Chemistry
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