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https://www.readbyqxmd.com/read/29216929/psat1-is-regulated-by%C3%A2-atf4-and-enhances-cell-proliferation-via-the-gsk3%C3%AE-%C3%AE-catenin-cyclin-d1-signaling-pathway-in-er-negative-breast-cancer
#1
Song Gao, Anqi Ge, Shouping Xu, Zilong You, Shipeng Ning, Yashuang Zhao, Da Pang
BACKGROUND: A growing amount of evidence has indicated that PSAT1 is an oncogene that plays an important role in cancer progression and metastasis. In this study, we explored the expression and function of PSAT1 in estrogen receptor (ER)-negative breast cancer. METHOD: The expression level of PSAT1 in breast cancer tissues and cells was analyzed using real-time-PCR (RT-PCR), TCGA datasets or immunohistochemistry (IHC). The overall survival of patients with ER-negative breast cancer stratified by the PSAT1 expression levels was evaluated using Kaplan-Meier analysis...
December 8, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29216580/microfluidic-magnetic-fluidized-bed-for-dna-analysis-in-continuous-flow-mode
#2
Iván Hernández-Neuta, Iago Pereiro, Annika Ahlford, Davide Ferraro, Qiongdi Zhang, Jean-Louis Viovy, Stéphanie Descroix, Mats Nilsson
Magnetic solid phase substrates for biomolecule manipulation have become a valuable tool for simplification and automation of molecular biology protocols. However, the handling of magnetic particles inside microfluidic chips for miniaturized assays is often challenging due to inefficient mixing, aggregation, and the advanced instrumentation required for effective actuation. Here, we describe the use of a microfluidic magnetic fluidized bed approach that enables dynamic, highly efficient and simplified magnetic bead actuation for DNA analysis in a continuous flow platform with minimal technical requirements...
November 26, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29214328/5-azacytidine-treatment-and-tapbf-d-over-expression-increases-glutenin-accumulation-within-the-wheat-grain-by-hypomethylating-the-glu-1-promoters
#3
Jiantang Zhu, Linlin Fang, Jiaqi Yu, Ying Zhao, Fanguo Chen, Guangmin Xia
5-azaC treatment and TaPBF - D over-expression decrease C-methylation status of three Glu - 1 gene promoters, and aid in enhancing the expression of the Glu - 1 genes. The wheat glutenins exert a strong influence over dough elasticity, but the regulation of their encoding genes has not been firmly established. Following treatment with 5-azacytidine (5-azaC), both the weight and glutenin content of the developing and mature grains were significantly increased. The abundance of transcript produced by the Glu-1 genes (encoding high-molecular-weight glutenin subunits), as well as those encoding demethylases and transcriptional factors associated with prolamin synthesis was higher than in grain of non-treated plants...
December 6, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29212199/klf4-reduces-stemness-phenotype-triggers-mesenchymal-epithelial-transition-met-like-molecular-changes-and-prevents-tumor-progression-in-nasopharygeal-carcinoma
#4
Xiqing Li, Zhunlan Zhao, Xiaoling Zhang, Sheng Yang, Xia Lin, Xinglong Yang, Xiaolin Lin, Junwen Shi, Shengchun Wang, Wentao Zhao, Jing Li, Fei Gao, Mingyue Liu, Ning Ma, Weiren Luo, Kaitai Yao, Yan Sun, Shengjun Xiao, Dong Xiao, Junshuang Jia
The reprogramming factor Krüppel-like factor 4 (Klf4), one of the Yamanaka's reprogramming factors, plays an essential role in reprogramming somatic cells into induced pluripotent stem cells (iPSCs). Klf4 is dysregulated and displays divergent functions in multiple malignancies, but the biological roles of Klf4 in nasopharyngeal carcinoma (NPC) remain unknown. The present study revealed that Klf4 downregulation in a cohort of human NPC biopsies is significantly associated with invasive and metastatic phenotypes of NPC...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29212168/nrf2-induces-cisplatin-resistance-via-suppressing-the-iron-export-related-gene-slc40a1-in-ovarian-cancer-cells
#5
Jianfa Wu, Lingjie Bao, Zhenbo Zhang, Xiaofang Yi
Induction of Nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) has been demonstrated to be involved in cisplatin resistance in ovarian cancer. Solute carrier family 40 member 1 (SLC40A1) is an iron exporter, which possesses many putative Nrf2 binding sites. Here we hypothesize that it may be a possible downstream gene of Nrf2. Elevated level of Nrf2 and reduced level of SLC40A1 were found in cisplatin-resistant ovarian cancer cells as compared with cisplatin-sensitive ovarian cancer cells. Exogenous knockdown of Nrf2 leaded to increased expression of SLC40A1...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29211985/the-rna-binding-site-of-poliovirus-3c-protein-doubles-as-a-phosphoinositide-binding-domain
#6
Djoshkun Shengjuler, Yan Mei Chan, Simou Sun, Ibrahim M Moustafa, Zhen-Lu Li, David W Gohara, Matthias Buck, Paul S Cremer, David D Boehr, Craig E Cameron
Some viruses use phosphatidylinositol phosphate (PIP) to mark membranes used for genome replication or virion assembly. PIP-binding motifs of cellular proteins do not exist in viral proteins. Molecular-docking simulations revealed a putative site of PIP binding to poliovirus (PV) 3C protein that was validated using nuclear magnetic resonance spectroscopy. The PIP-binding site was located on a highly dynamic α helix, which also functions in RNA binding. Broad PIP-binding activity was observed in solution using a fluorescence polarization assay or in the context of a lipid bilayer using an on-chip, fluorescence assay...
December 5, 2017: Structure
https://www.readbyqxmd.com/read/29211822/insights-from-resolving-protein-dna-interactions-at-near-base-pair-resolution
#7
Bryan J Venters
One of the central goals in molecular biology is to understand how cell-type-specific expression patterns arise through selective recruitment of RNA polymerase II (Pol II) to a subset of gene promoters. Pol II needs to be recruited to a precise genomic position at the proper time to produce messenger RNA from a DNA template. Ostensibly, transcription is a relatively simple cellular process; yet, experimentally measuring and then understanding the combinatorial possibilities of transcriptional regulators remain a daunting task...
December 2, 2017: Briefings in Functional Genomics
https://www.readbyqxmd.com/read/29211088/multi-size-spheroid-formation-using-microfluidic-funnels
#8
M Marimuthu, N Rousset, A St-Georges-Robillard, M A Lateef, M Ferland, A-M Mes-Masson, T Gervais
We present a microfluidic platform for automatic multi-size spheroid formation within constant volume hanging droplets (HDs) from a single inlet loading of a constant cell concentration. The platform introduces three technological improvements over the existing spheroid formation platforms: 1) cell seeding control is achieved by enrichment of a cell solution rather than dilution; 2) cell seeding in each HD is fully independent and pre-programmable at the design stage; 3) the fabricated chip operates well using a hydrophobic PDMS surface, ensuring long-term storage possibility for device usage...
December 6, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29208528/heterochromatin-protein-hp-1%C3%AE-is-not-only-in-the-nucleus-but-also-in-the-cytoplasm-interacting-with-actin-in-both-cell-compartments
#9
Nancy L Charó, Natalia M Galigniana, Graciela Piwien-Pilipuk
Confocal and electron microscopy images, and WB analysis of cellular fractions revealed that HP1γ is in the nucleus but also in the cytoplasm of C2C12 myoblasts, myotubes, skeletal and cardiac muscles, N2a, HeLa and HEK293T cells. Signal specificity was tested with different antibodies and by HP1γ knockdown. Leptomycin B treatment of myoblasts increased nuclear HP1γ, suggesting that its nuclear export is Crm-1-dependent. HP1γ exhibited a filamentous pattern of staining partially co-localizing with actin in the cytoplasm of myotubes and myofibrils...
December 2, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29207671/ginkgo-biloba-extract-egb-761-induced-upregulation-of-lincrna-p21-inhibits-colorectal-cancer-metastasis-by-associating-with-ezh2
#10
Tingting Liu, Junzhong Zhang, Zhongqiu Chai, Gang Wang, Naiqiang Cui, Bing Zhou
EGb 761, the standard ginkgo biloba extract, is frequently prescribed in traditional Chinese medicine. Currently, there is no research focusing on its role in human colorectal cancer progression. In our study, we determined the anti-metastatic effect of EGb 761 on colorectal cancer cells and further explored the potential underlying regulatory mechanism. The cell migration and invasion assay indicated that EGb 761 treatment of colorectal cancer cells induced inhibition of cell migration and invasion ability in a concentration-dependent manner...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29207655/egr-1-regulates-rta-transcription-through-a-cooperative-involvement-of-transcriptional-regulators
#11
Roni Sarkar, Subhash C Verma
Kaposi's sarcoma associated herpesvirus (KSHV) regulates the host cellular environment to establish life-long persistent infection by manipulating cellular signaling pathways, with approximately 1- 5% of cells undergoing lytic reactivation during the course of infection. Egr-1 (Early Growth Response Factor-1) is one such cellular transcription factor, which gets phosphorylated during the lytic phase of viral life cycle to perpetrate its function. This study demonstrates the mechanism of how Egr-1 mediates transcription of the immediate early gene, RTA (Replication and transcription activator), which is the lytic switch gene of KSHV...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29206205/an-all-glass-microfluidic-network-with-integrated-amorphous-silicon-photosensors-for-on-chip-monitoring-of-enzymatic-biochemical-assay
#12
Francesca Costantini, Roald M Tiggelaar, Riccardo Salvio, Marco Nardecchia, Stefan Schlautmann, Cesare Manetti, Han J G E Gardeniers, Giampiero de Cesare, Domenico Caputo, Augusto Nascetti
A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on the sealed microfluidic substrate preventing the contamination of the micro-channels. The microfluidic network is then made accessible by opening inlets and outlets just prior to the use, ensuring the sterility of the device...
December 5, 2017: Biosensors
https://www.readbyqxmd.com/read/29204837/current-technologies-based-on-the-knowledge-of-the-stem-cells-microenvironments
#13
Damia Mawad, Gemma Figtree, Carmine Gentile
The stem cell microenvironment or niche plays a critical role in the regulation of survival, differentiation and behavior of stem cells and their progenies. Recapitulating each aspect of the stem cell niche is therefore essential for their optimal use in in vitro studies and in vivo as future therapeutics in humans. Engineering of optimal conditions for three-dimensional stem cell culture includes multiple transient and dynamic physiological stimuli, such as blood flow and tissue stiffness. Bioprinting and microfluidics technologies, including organs-on-a-chip, are among the most recent approaches utilized to replicate the three-dimensional stem cell niche for human tissue fabrication that allow the integration of multiple levels of tissue complexity, including blood flow...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29202812/celastrol-improves-self-renewal-and-differentiation-of-human-tendon-derived-stem-cells-by-suppressing-smad7-through-hypoxia
#14
Tianyi Wu, Shenghe Liu, Gen Wen, Jia Xu, Yaling Yu, Yimin Chai
BACKGROUND: We aimed to evaluate the potential enhancing effect of celastrol on the stemness of human tendon-derived stem cells (hTSCs) in vitro and the underlying molecular mechanisms. METHODS: The capability of hTSC self-renewal was assessed by cell proliferation and colony formation as determined with the CCK-8 kit. Adipogenesis, chondrogenesis, and osteogenesis were determined by Oil Red O, Alcian Blue, and Alizarin Red staining, respectively. The relative mRNA levels of Sox9, PPARγ, Runx2, Smad7, and HIF1α were determined by real-time polymerase chain reaction (PCR)...
December 4, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29200969/inhibition-of-breast-cancer-cell-proliferation-and-tumorigenesis-by-long-non-coding-rna-rpph1-down-regulation-of-mir-122-expression
#15
Yi Zhang, Lili Tang
Background: Recent studies showed that long non-coding RNA (lncRNA) plays an important role in many life activities. RPPH1 is one of the lncRNA genes that are expressed differently between breast cancer and normal tissues by the lncRNA gene chip. Our study was conducted to examine the regulation of lncRNA RPPH1 in breast cancer. Methods: Two cell lines, MCF-7 and MDA-MB-231, were selected to be the research objects in this study; RPPH1 overexpression and knockdown models were established by transforming vectors...
2017: Cancer Cell International
https://www.readbyqxmd.com/read/29199733/a-robust-portable-and-backflow-free-micromixing-device-based-on-both-capillary-and-vacuum-driven-flows
#16
Yaguang Zhai, Anyang Wang, Domin Koh, Philip Schneider, Kwang W Oh
In capillary- or vacuum-driven microfluidics, surge backflow events are common when merging or pumping two similar or dissimilar liquids together if a pressure difference exists between them. In this work, a robust, portable micromixing device that is insensitive to backflow was designed, fabricated and characterised. A capillary-driven pressure balancing bypass connected between two inlet ports diminished the initial pressure difference caused by capillarity and gravity present in each liquid at the two inlet ports...
December 4, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29198118/rapid-and-quantitative-detection-of-vibrio-parahemolyticus-by-the-mixed-dye-based-loop-mediated-isothermal-amplification-assay-on-a-self-priming-compartmentalization-microfluidic-chip
#17
Bo Pang, Xiong Ding, Guoping Wang, Chao Zhao, Yanan Xu, Kaiyue Fu, Jingjing Sun, Xiuling Song, Wenshuai Wu, Yushen Liu, Qi Song, Jiumei Hu, Juan Li, Ying Mu
Vibrio parahemolyticus (VP) mostly isolated from aquatic products is one of the major causes of bacterial food poisoning events worldwide, which could be reduced using a promising on-site detection method. Herein, a rapid and quantitative method for VP detection was developed by applying mixed-dye-loaded loop-mediated isothermal amplification (LAMP) assay on self-priming compartmentalization (SPC) microfluidic chip, termed on-chip mixed-dye-based LAMP (CMD-LAMP). Compared to conventional approaches, CMD-LAMP was advantageous on the limit of detection which reached down to 1×103 CFU/ mL in food contaminated samples without the pre-enrichment of bacteria...
December 3, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29197187/development-of-a-microfluidic-platform-for-high-throughput-screening-of-non-viral-gene-delivery-vectors
#18
Elisa Giupponi, Roberta Visone, Paola Occhetta, Federica Colombo, Marco Rasponi, Gabriele Candiani
The grail of gene delivery is the development of delivery vectors as effective and non-cytotoxic as possible. In this regard, there is an urgent need of new tools for the straightforward and quantitative assessment of transfection efficiency and cytotoxicity simultaneously. We herein reported the development and validation of an easy-to-use lab-on-chip platform to perform cell transfection assays for unbiased, high-throughput selection of more and more effective gene delivery vectors by using 2 commercially sourced lipids, Lipofectamine 2000® and FuGene® 6...
December 2, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29196315/phosphorylated-ccaat-enhancer-binding-protein-%C3%AE-contributes-to-rat-hiv-related-neuropathic-pain-in-vitro-and-in-vivo-studies
#19
Hyun Yi, Shue Liu, Yuta Kashiwagi, Daigo Ikegami, Wan Huang, Hirotsugu Kanda, Takafumi Iida, Ching-Hang Liu, Keiya Takahashi, David A Lubarsky, Shuanglin Hao
Chronic pain is increasingly recognized as an important comorbidity of HIV-infected patients, however, the exact molecular mechanisms of HIV-related pain are still elusive. CCAAT/enhancer binding proteins (C/EBPs) are expressed in various tissues, including the central nervous system. C/EBPβ, one of C/EBPs is involved in the progression of HIV/AIDS, but the exact role of C/EBPβ and its upstream factors are not clear in HIV pain state. Here, we used a neuropathic pain model of perineural HIV envelope glycoprotein gp120 application onto the rat sciatic nerve to test the role of phosphorylated C/EBPβ (pC/EBPβ) and its upstream pathway in the spinal cord dorsal horn (SCDH)...
December 1, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29195126/stimulation-of-interferon-stimulated-gene-20-by-thyroid-hormone-enhances-angiogenesis-in-liver-cancer
#20
Syuan-Ling Lin, Sheng-Ming Wu, I-Hsiao Chung, Yang-Hsiang Lin, Ching-Ying Chen, Hsiang-Cheng Chi, Tzu-Kang Lin, Chau-Ting Yeh, Kwang-Huei Lin
Thyroid hormone, 3,3',5-triiodo-l-thyronine (T3), mediates several physiological processes, including embryonic development, cellular differentiation and cell proliferation, via binding to its nuclear thyroid receptors (TR). Previous microarray and Chromatin immunoprecipitation (ChIP)-on-ChIP analyses have revealed that interferon-stimulated gene 20 kDa (ISG20), an exoribonuclease involved in the antiviral function of interferon, is up-regulated by T3 in HepG2-TR cells. However, the underlying mechanisms of ISG20 action in tumor progression remain unknown to date...
November 28, 2017: Neoplasia: An International Journal for Oncology Research
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