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NF-KB Transcriptional regulation

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https://www.readbyqxmd.com/read/29148034/role-of-p62-sqstm1-beyond-autophagy-a-lesson-learned-from-drug-induced-toxicity-in-vitro
#1
Fernando Alegre, Ángela B Moragrega, Miriam Polo, Alberto Marti-Rodrigo, Juan V Esplugues, Ana Blas-Garcia, Nadezda Apostolova
BACKGROUND AND PURPOSE: SQSTM1/p62 is a multifunctional, stress-induced, scaffold protein involved in multiple cellular processes including autophagic clearance, regulation of inflammatory responses and redox homeostasis. Alterations in its function have been associated with a long list of human pathologies such as neurodegenerative, metabolic and bone diseases (down-regulation), and cancerogenesis (up-regulation). However, its role in the off-target effects of clinically used drugs is still not understood...
November 17, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29115856/lps-induced-acute-lung-injury-involves-the-nf-%C3%AE%C2%BAb-mediated-downregulation-of-sox18
#2
Christine M Gross, Manuela Kellner, Ting Wang, Qing Lu, Xutong Sun, Evgeny A Zemskov, Satish Noonepalle, Archana Kangath, Sanjiv Kumar, Manuel Gonzalez-Garay, Ankit A Desai, Saurabh Aggarwal, Boris Gorshkov, Christina Klinger, Alexander D Verin, John D Catravas, Jeffrey R Jacobson, Jason X-J Yuan, Ruslan Rafikov, Joe G N Garcia, Stephen M Black
One of the early events in the progression of lipopolysaccharide (LPS)-mediated acute lung injury (ALI) in mice is the disruption of the pulmonary endothelial barrier resulting in lung edema. However, the molecular mechanisms by which the endothelial barrier becomes compromised remain unresolved. The SRY-related High Mobility Group box (Sox) group-F family member, Sox18, is a barrier- protective protein through its ability to increase the expression of the tight junction protein, Claudin-5. Thus, the purpose of this study was to determine if down-regulation of the Sox18-Claudin-5 axis plays a role in the pulmonary endothelial barrier disruption associated with LPS exposure...
November 8, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/29100435/effect-of-talen-mediated-il-6-knockout-on-cell-proliferation-apoptosis-invasion-and-anti-cancer-therapy-in-hepatocellular-carcinoma-hcc-lm3-cells
#3
Peng-Yuan Zhuang, Ke-Wei Zhang, Jian-Dong Wang, Xue-Ping Zhou, Ying-Bin Liu, Zhi-Wei Quan, Jun Shen
Purpose: To determine the exact effect of Interleukin-6 (IL-6) on tumor cell proliferation, apoptosis, invasion, and anti-cancer therapy in hepatocellular carcinoma (HCC). Experimental Design: IL-6 was disrupted by transcription activator-like effector nucleases (TALEN) in HCCLM3 cells, and was used to evaluate the role of IL-6 on tumor cell proliferation, apoptosis, invasion and key signaling pathways involved in sorafenib and/or IFNα therapy. Results: IL-6 has no direct effect on cell proliferation and invasion but promotes cell apoptosis and up-regulate IL-33 and VEGF-A expression...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29100036/nafamostat-mesilate-a-serine-protease-inhibitor-suppresses-interferon-gamma-induced-up-regulation-of-programmed-cell-death-ligand-1-in-human-cancer-cells
#4
Sadamu Homma, Kazumi Hayashi, Kosaku Yoshida, Yukiko Sagawa, Yuko Kamata, Masaki Ito
Programmed cell death ligand-1 (PD-L1) plays a pivotal role in the suppression of antitumour immunity by binding to programmed cell death-1 (PD-1) on tumouricidal cytotoxic T lymphocytes (CTLs), rendering them inactive. As blockade of PD-1/PD-L1 interaction by the monoclonal antibodies induced effective T cell-mediated antitumour response, suppression of PD-L1 expression in tumour cells by the chemical agent might contribute to treatment against malignant tumours. Nafamostat mesilate (NM), a serine protease inhibitor that is frequently used in the clinic, potently suppressed interferon-gamma (IFN-gamma)-induced up-regulation of PD-L1 in cultured human lung cancer cells (HLC-1) at both the messenger RNA (mRNA) and protein levels...
October 28, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/29074539/nf-kb-promotes-ovarian-tumorigenesis-via-classical-pathways-supporting-proliferative-cancer-cells-and-alternative-pathways-supporting-aldh-cancer-stem-like-cells
#5
Carrie D House, Elizabeth Jordan, Lidia Hernandez, Michelle Ozaki, Jana M James, Marianne Kim, Michael J Kruhlak, Eric Batchelor, Fathi Elloumi, Margaret C Cam, Christina M Annunziata
Understanding the mechanisms supporting tumor-initiating cells (TIC) is vital to combat advanced stage recurrent cancers. Here we show that in advanced ovarian cancers NF-kB signaling via the RelB transcription factor supports TIC populations by directly regulating the cancer stem-like associated enzyme aldehyde dehydrogenase (ALDH). Loss of RelB significantly inhibited spheroid formation, ALDH expression and activity, chemoresistance, and tumorigenesis in subcutaneous and intrabursal mouse xenograft models of human ovarian cancer...
October 26, 2017: Cancer Research
https://www.readbyqxmd.com/read/29051223/crispr-cas9-based-screening-of-long-noncoding-rnas-lncrnas-in-macrophages-with-an-nf-kappa-b-reporter
#6
Sergio Covarrubias, Elektra K Robinson, Barbara Shapleigh, Apple Vollmers, Solomon Katzman, Nicole Hanley, Nicholas Fong, Michael T McManus, Susan Carpenter
The innate immune system protects against infections by initiating an inducible inflammatory response. NF-kappa B (NF-KB) is one of the critical transcription factors controlling this complex response, but some aspects of its regulation remain unclear. For example, although long noncoding RNAs (lncRNAs) have been shown to critically regulate gene expression, only a fraction of these have been functionally characterized, and the extent to which lncRNAs control NF-KB expression is unknown. Here, we describe the generation of a GFP-based NF-KB reporter system in immortalized murine bone marrow-derived macrophages (iBMDMs)...
October 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29047264/the-inactivation-of-erk1-2-p38-and-nf-kb-is-involved-in-the-down-regulation-of-osteoclastogenesis-and-function-by-a2b-adenosine-receptor-stimulation
#7
Bo Hyun Kim, Ju Hee Oh, Na Kyung Lee
A2B adenosine receptor (A2BAR) is known to be the regulator of bone homeostasis, but its regulatory mechanisms in osteoclast formation are less well-defined. Here, we demonstrate the effect of A2BAR stimulation on osteoclast differentiation and activity by RANKL. A2BAR was expressed in bone marrow-derived monocyte/macrophage (BMM) and RANKL increased A2BAR expression during osteoclastogenesis. A2BAR stimulation with its specific agonist BAY 60-6583 was sufficient to inhibit the activation of ERK1/2, p38 MAP kinases and NF-κB by RANKL as well as it abrogated cell-cell fusion in the late stage of osteoclast differentiation...
October 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29046054/porphyromonas-gingivalis-lipopolysaccharide-stimulation-in-human-periodontal-ligament-stem-cells-role-of-epigenetic-modifications-to-the-inflammation
#8
Francesca Diomede, Soundara Rajan Thangavelu, Ilaria Merciaro, Monica D'Orazio, Placido Bramanti, Emanuela Mazzon, Oriana Trubiani
Periodontitis is a chronic oral inflammatory disease produced by bacteria. Gingival retraction and bone and connective tissues resorption are the hallmarks of this disease. Chronic periodontitis may contribute to the risk of onset or progression of neuroinflammatory pathological conditions, such as Alzheimer's disease. The main goal of the present study was to investigate if the role of epigenetic modulations is involved in periodontitis using human periodontal ligament stem cells (hPDLSCs) as an in vitro model system...
August 25, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28983059/pro-inflammatory-proteins-s100a9-and-tnf%C3%AE-suppress-erythropoietin-elaboration-in-myelodysplastic-syndromes
#9
Thomas Cluzeau, Kathy L McGraw, Brittany Irvine, Erico Masala, Lionel Ades, Ashley A Basiorka, Jaroslaw P Maciejewski, Patrick Auberger, Sheng Wei, Pierre Fenaux, Valeria Santini, Alan List
Accumulating evidence implicates innate immune activation in the pathobiology of myelodysplastic syndromes. A key myeloid related inflammatory protein, S100A9, serves as a Toll-like receptor ligand regulating TNFα and IL-1β production. The role of MDS-related inflammatory proteins in endogenous Epo regulation and response to erythroid stimulating agents or lenalidomide has not been investigated. The HepG2 hepatoma cell line was used to investigate in vitro Epo elaboration. Serum collected from 311 MDS patients were investigated (125 prior to erythroid stimulating agents and 186 prior to lenalidomide)...
October 5, 2017: Haematologica
https://www.readbyqxmd.com/read/28967880/nlrx1-promotes-immediate-irf1-directed-antiviral-responses-by-limiting-dsrna-activated-translational-inhibition-mediated-by-pkr
#10
Hui Feng, Erik M Lenarcic, Daisuke Yamane, Eliane Wauthier, Jinyao Mo, Haitao Guo, David R McGivern, Olga González-López, Ichiro Misumi, Lola M Reid, Jason K Whitmire, Jenny P-Y Ting, Joseph A Duncan, Nathaniel J Moorman, Stanley M Lemon
NLRX1 is unique among the nucleotide-binding-domain and leucine-rich-repeat (NLR) proteins in its mitochondrial localization and ability to negatively regulate antiviral innate immunity dependent on the adaptors MAVS and STING. However, some studies have suggested a positive regulatory role for NLRX1 in inducing antiviral responses. We found that NLRX1 exerted opposing regulatory effects on viral activation of the transcription factors IRF1 and IRF3, which might potentially explain such contradictory results...
December 2017: Nature Immunology
https://www.readbyqxmd.com/read/28941036/association-of-donor-sumo4-rs237025-genetic-variant-with-tacrolimus-elimination-in-the-early-period-after-liver-transplantation
#11
Tao Zhang, Yuan Liu, Rong Zeng, Qi Ling, Peihao Wen, Junwei Fan, Zhihai Peng
BACKGROUNDS & AIMS: Individualized tacrolimus treatment can improve drug safety and efficacy. In this study, we aimed to investigate the association of donor and recipient small ubiquitin-like modifier 4 (SUMO4) rs237025 polymorphisms with tacrolimus elimination and the potential mechanism. METHODS: A total of 297 liver transplant patients were enrolled in the study. CYP3A5 rs776746 and SUMO4 rs237025 were genotyped using TaqMan SNPs assays. The activity of nuclear factor-kB (NF-kB) was evaluated by luciferase assay...
September 21, 2017: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/28928248/carbonic-anhydrase-iii-protects-osteocytes-from-oxidative-stress
#12
Chao Shi, Yuhei Uda, Christopher Dedic, Ehab Azab, Ningyuan Sun, Amira I Hussein, Christopher A Petty, Keertik Fulzele, Maria C Mitterberger-Vogt, Werner Zwerschke, Renata Pereira, Kunzheng Wang, Paola Divieti Pajevic
Osteocytes are master orchestrators of bone remodeling; they control osteoblast and osteoclast activities both directly via cell-to-cell communication and indirectly via secreted factors, and they are the main postnatal source of sclerostin and RANKL (receptor activator of NF-kB ligand), two regulators of osteoblast and osteoclast function. Despite progress in understanding osteocyte biology and function, much remains to be elucidated. Recently developed osteocytic cell lines-together with new genome editing tools-has allowed a closer look at the biology and molecular makeup of these cells...
September 19, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28883651/the-role-of-transcriptional-factor-p63-in-regulation-of-epithelial-barrier-and-ciliogenesis-of-human-nasal-epithelial-cells
#13
Yakuto Kaneko, Takayuki Kohno, Takuya Kakuki, Ken-Ichi Takano, Noriko Ogasawara, Ryo Miyata, Shin Kikuchi, Takumi Konno, Tsuyoshi Ohkuni, Ryoto Yajima, Akito Kakiuchi, Shin-Ichi Yokota, Tetsuo Himi, Takashi Kojima
Disruption of nasal epithelial tight junctions (TJs) and ciliary dysfunction are found in patients with chronic rhinosinusitis (CRS) and nasal polyps (NPs), along with an increase of p63-positive basal cells and histone deacetylase (HDAC) activity. To investigate these mechanisms, primary cultures of HNECs transfected with human telomerase reverse transcriptase (hTERT-HNECs) were transfected with siRNAs of TAp63 and ΔNp63, treated with the NF-kB inhibitor curucumin and inhibitors of HDACs, and infected with respiratory syncytial virus (RSV)...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28878257/molecular-mechanisms-governing-microrna-125a-expression-in-human-hepatocellular-carcinoma-cells
#14
Nicoletta Potenza, Marta Panella, Filomena Castiello, Nicola Mosca, Elena Amendola, Aniello Russo
MicroRNA-125a-5p (miR-125a) is a vertebrate homolog of lin-4, the first discovered microRNA, and plays a fundamental role in embryo development by downregulating Lin-28 protein. MiR-125a is also expressed in differentiated cells where it generally acts as an antiproliferative factor by targeting membrane receptors or intracellular transductors of mitogenic signals. MiR-125a expression is downregulated in several tumors, including hepatocellular carcinoma (HCC) where it targets sirtuin-7, matrix metalloproteinase-11, VEGF-A, Zbtb7a, and c-Raf...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28870864/counter-regulation-of-ecrg4-gene-expression-by-hypermethylation-dependent-inhibition-and-the-sp1-transcription-factor-dependent-stimulation-of-the-c2orf40-promoter
#15
Xitong Dang, Xiaorong Zeng, Raul Coimbra, Brian P Eliceiri, Andrew Baird
The human cytokine precursor ECRG4 has been associated with multiple physiological, developmental and pathophysiological processes involving cell proliferation, cell migration, innate immunity, inflammation, cancer progression and metastases. Although down-regulation of ECRG4 gene expression has been largely attributed to hypermethylation of CpG islands in the 5'untranslated region of the ECRG4 promoter, the mechanisms that underlie the dynamics of its regulation have never been systematically described. Here we show that the ECRG4 gene is widely expressed in human tissues and report that its core promoter lies between the -780 to +420 base pairs relative to the ATG start codon of the ECRG4 open reading frame...
December 15, 2017: Gene
https://www.readbyqxmd.com/read/28859984/epstein-barr-virus-encoded-latent-membrane-protein-1-induces-epithelial-to-mesenchymal-transition-by-inducing-v-set-ig-domain-containing-4-vsig4-expression-via-nf-kb-in-renal-tubular-epithelial-hk-2%C3%A2-cells
#16
Seung-Mi Kim, Se Won Oh, Sang Hyun Park, Dae Young Hur, Seung-Woo Hong, Sang Youb Han
The epithelial to mesenchymal transition (EMT), a hallmark of chronic kidney disease, is a key event in the conversion from tubular epithelial cells to myofibroblasts in renal fibrosis. Epstein-Barr virus (EBV) is a γ-herpes oncovirus associated with chronic kidney disease. However, the relationship between EBV and the EMT process in renal tubular epithelial cells is not well understood. Among EBV-latent genes, EBV-encoded latent membrane protein 1 (LMP1) induces EMT by regulating a variety of molecules in EBV-induced oncogenic transformation...
October 21, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28840846/tumour-necrosis-factor-alpha-and-nuclear-factor-kappa-b-gene-variants-in-sepsis
#17
Leyla Acar, Nazan Atalan, E Hande Karagedik, Arzu Ergen
BACKGROUND: Humoral system is activated and various cytokines are released due to infections in tissues and traumatic damage. NF-kB dimers are encoded by NF-kB genes and regulate transcription of several crucial proteins of inflammation such as tumour necrosis factor-alpha (TNFα). AIMS: To investigate the possible effect of polymorphisms on TNFα serum levels with clinical and prognostic parameters of sepsis by determining the NF-kB1 -94 ins/del ATTG and TNFα (-308 G/A) gene polymorphisms and TNFα serum levels...
August 25, 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/28827109/chromofungin-chr-chga47-66-is-downregulated-in-persons-with-active-ulcerative-colitis-and-suppresses-pro-inflammatory-macrophage-function-through-the-inhibition-of-nf-%C3%AE%C2%BAb-signaling
#18
Nour Eissa, Hayam Hussein, Laëtitia Kermarrec, Omar Elgazzar, Marie-Helene Metz-Boutigue, Charles N Bernstein, Jean-Eric Ghia
Chromogranin-A (CHGA) is a prohormone secreted by neuroendocrine cells and is a precursor of several bioactive peptides, which are implicated in different and distinctive biological and immune functions. Chromofungin (CHR: CHGA47-66) is a short peptide with antimicrobial effects and encodes from CHGA exon-IV. Inflammatory bowel disease (IBD) is characterized by alterations in the activation of pro-inflammatory pathways, pro-inflammatory macrophages (M1), and nuclear transcription factor kappa B (NF-κB) signaling leading to the perpetuation of the inflammatory process...
August 19, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28803264/hyaluronan-in-the-experimental-injury-of-the-cartilage-biochemical-action-and-protective-effects
#19
REVIEW
Angela Avenoso, Angela D'Ascola, Michele Scuruchi, Giuseppe Mandraffino, Alberto Calatroni, Antonino Saitta, Salvatore Campo, Giuseppe M Campo
INTRODUCTION: Our knowledge of extracellular matrix (ECM) structure and function has increased enormously over the last decade or so. There is evidence demonstrating that ECM provides signals affecting cell adhesion, shape, migration, proliferation, survival, and differentiation. ECM presents many domains that become active after proteolytic cleavage. These active ECM fragments are called matrikines which play different roles; in particular, they may act as potent inflammatory mediators during cartilage injury...
August 12, 2017: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/28748217/mechanisms-and-applications-of-the-anti-inflammatory-effects-of-photobiomodulation
#20
Michael R Hamblin
Photobiomodulation (PBM) also known as low-level level laser therapy is the use of red and near-infrared light to stimulate healing, relieve pain, and reduce inflammation. The primary chromophores have been identified as cytochrome c oxidase in mitochondria, and calcium ion channels (possibly mediated by light absorption by opsins). Secondary effects of photon absorption include increases in ATP, a brief burst of reactive oxygen species, an increase in nitric oxide, and modulation of calcium levels. Tertiary effects include activation of a wide range of transcription factors leading to improved cell survival, increased proliferation and migration, and new protein synthesis...
2017: AIMS Biophysics
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