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https://www.readbyqxmd.com/read/29777762/astrocyte-elevated-gene-1-is-a-novel-regulator-of-astrogliosis-and-excitatory-amino-acid-transporter-2-via-interplaying-with-nuclear-factor-%C3%AE%C2%BAb-signaling-in-astrocytes-from-amyotrophic-lateral-sclerosis-mouse-model-with-hsod1-g93a-mutation
#1
Xiang Yin, Shuyu Wang, Yan Qi, Xudong Wang, Hongquan Jiang, Tianhang Wang, Yueqing Yang, Ying Wang, Chunting Zhang, Honglin Feng
AEG-1 has received extensive attention on cancer research. However, little is known about its roles in astrogliosis of Amyotrophic lateral sclerosis (ALS). In this study, we detected AEG-1 expression in hSOD1G93A -positive (mut-SOD1) astrocytes and wild type (wt-SOD1) astrocytes, and intend to elucidate its potential functions in ALS related astrogliosis and the always accompanied dysregulated glutamate clearance. Results showed elevated protein and mRNA levels of AEG-1 in mut-SOD1 astrocytes; Also, NF-κB signaling pathway related proteins and inflammatory cytokines were upregulated in mut-SOD1 astrocytes; AEG-1 knockdown attenuated astrocytes proliferation and pro-inflammatory release; also we found that AEG-1 silence inhibited translocation of p65 from cytoplasma to nuclear, which was associated with inhibited NF-κB signaling...
May 16, 2018: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/29776446/valproic-acid-attenuates-traumatic-spinal-cord-injury-induced-inflammation-via-stat1-and-nf-%C3%AE%C2%BAb-pathway-dependent-of-hdac3
#2
Shoubo Chen, Jingfang Ye, Xiangrong Chen, Jinnan Shi, Wenhua Wu, Wenping Lin, Weibin Lin, Yasong Li, Huangde Fu, Shun Li
BACKGROUND: Microglial polarization with M1/M2 phenotype shifts and the subsequent neuroinflammatory responses are vital contributing factors for spinal cord injury (SCI)-induced secondary injury. Nuclear factor-κB (NF-κB) is considered the central transcription factor of inflammatory mediators, which plays a crucial role in microglial activation. Lysine acetylation of STAT1 seems necessary for NF-kB pathway activity, as it is regulated by histone deacetylases (HDACs). There have been no studies that have explained if HDAC inhibition by valproic acid (VPA) affects the NF-κB pathway via acetylation of STAT1 dependent of HDAC activity in the microglia-mediated central inflammation following SCI...
May 18, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29775759/-938-rhrk-941-is-responsible-for-ubiquitin-specific-protease-48-nuclear-translocation-which-can-stabilize-nf-%C3%AE%C2%BAb-p65-in-the-nucleus
#3
Ying-Li Liu, Jing Yan, Jie Zheng, Qing-Bao Tian
The transcription factor NF-κB is a key regulator of cellular processes. A mechanism that contributes to timely termination of NF-κB activity is UPS-dependent degradation of p65 in the nucleus or on chromatin. The ubiquitin-specific protease that takes part in this process and its molecular mechanisms are shown in previous study, but which structural feature of USP48 was responsible for these effects is unknown. Here, we show that maybe the stability of NF-κB is controlled by proteasome-mediated degradation and ubiquitin-specific protease 48 (USP48), also known as synaptic ubiquitin-specific protease (synUSP) or USP31, can enhance NF-κB stability through proteasome-dependent regulation in the nucleus...
May 15, 2018: Gene
https://www.readbyqxmd.com/read/29773986/flavopiridol-protects-bone-tissue-by-attenuating-rankl-induced-osteoclast-formation
#4
Zi'ang Hu, Yilei Chen, Lijiang Song, Jasper H N Yik, Dominik R Haudenschild, Shunwu Fan
Bone resorption and homeostasis is carried out by osteoclasts, whose differentiation and activity are regulated by the RANK/RANKL axis. Our previous studies using a mouse model of joint injury show that joint trauma induces local inflammation followed by bone remodeling. The transcription factor cyclin-dependent kinase 9 (CDK9) is the major regulator of inflammation, as CDK9 inhibitor flavopiridol effectively suppress injury-induced inflammatory response. The objective of this study was to investigate the underlying mechanism through which flavopiridol regulates bone resorption...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29768255/asiatic-acid-aa-sensitizes-multidrug-resistant-human-lung-adenocarcinoma-a549-ddp-cells-to-cisplatin-ddp-via-downregulation-of-p-glycoprotein-mdr1-and-its-targets
#5
Qilai Cheng, Meixiang Liao, Haibo Hu, Hongliang Li, Longhuo Wu
BACKGROUND/AIMS: P-glycoprotein (P-gp, i.e., MDR1) is associated with the phenotype of multidrug resistance (MDR) and causes chemotherapy failure in the management of cancers. Searching for effective MDR modulators and combining them with anticancer drugs is a promising strategy against MDR. Asiatic acid (AA), a natural triterpene isolated from the plant Centella asiatica, may have an antitumor activity. The present study assessed the reversing effect of AA on MDR and possible molecular mechanisms of AA action in MDR1-overexpressing cisplatin (DDP)-resistant lung cancer cells, A549/DDP...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29767264/elemental-diet-inhibits-pro%C3%A2-inflammatory-cytokine-production-in-keratinocytes-through-the-suppression-of-nf%C3%A2-%C3%AE%C2%BAb-activation
#6
Koji Harada, Tarannum Ferdous, Yoichi Mizukami, Katsuaki Mishima
An elemental diet (ED) has been reported to reduce oral mucositis and dermatitis induced by chemotherapy. However, its molecular mechanism of action as an anti‑inflammatory agent is still unknown. The aim of the present study was to clarify whether ED confers its anti‑inflammatory action via reduction of pro‑inflammatory cytokine production in keratinocytes in vivo and in vitro. We evaluated the efficacy of ED in the treatment of 5‑fluorouracil (5‑FU)‑induced dermatitis of nude mice, and examined the expression of pro‑inflammatory cytokines such as tumor necrosis factor‑α (TNF‑α), interleukin (IL)‑1β and IL‑6 using immunohistochemistry...
May 16, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29758489/fisetin-inhibits-the-generation-of-inflammatory-mediators-in-interleukin-1%C3%AE-induced-human-lung-epithelial-cells-by-suppressing-the-nf-%C3%AE%C2%BAb-and-erk1-2-pathways
#7
Hui-Ling Peng, Wen-Chung Huang, Shu-Chen Cheng, Chian-Jiun Liou
Fisetin, a flavone that can be isolated from fruits and vegetables, has anti-tumor and anti-oxidative properties and ameliorates airway hyperresponsiveness in asthmatic mice. This study investigated whether fisetin can suppress the expression of inflammatory mediators and intercellular adhesion molecule 1 (ICAM-1) in A549 human lung epithelial cells that were stimulated with interleukin-1β (IL-1β) to induce inflammatory responses. A549 cells were treated with fisetin (3-30 μM) and then with IL-1β. Fisetin significantly inhibited COX-2 expression and reduced prostaglandin E2 production, and it suppressed the levels of IL-8, CCL5, monocyte chemotactic protein 1, tumor necrosis factor α, and IL-6...
May 11, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29755455/rnai-based-identification-of-gene-specific-nuclear-cofactor-networks-regulating-interleukin-1-target-genes
#8
Johanna Meier-Soelch, Liane Jurida, Axel Weber, Doris Newel, Johnny Kim, Thomas Braun, M Lienhard Schmitz, Michael Kracht
The potent proinflammatory cytokine interleukin (IL)-1 triggers gene expression through the NF-κB signaling pathway. Here, we investigated the cofactor requirements of strongly regulated IL-1 target genes whose expression is impaired in p65 NF-κB-deficient murine embryonic fibroblasts. By two independent small-hairpin (sh)RNA screens, we examined 170 genes annotated to encode nuclear cofactors for their role in Cxcl2 mRNA expression and identified 22 factors that modulated basal or IL-1-inducible Cxcl2 levels...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29748072/nicorandil-reduces-burn-wound-progression-by-enhancing-skin-blood-flow
#9
Jaehoon Choi, Ryeolwoo Kim, Junhyung Kim, Woonhyeok Jeong, Sang Woo Park, Hyoun Wook Lee, Ho Yun Chung
We assessed whether nicorandil, an adenosine triphosphate-sensitive K + channel opener, reduces burn wound progression in a rat comb burn model. A total of 24 rats were used. Following thermal injury, one dose of nicorandil (10 or 30 mg/kg) was administered intragastrically twice daily for 3 days. At days 1 and 3 after injury, skin was harvested for histopathological examination and protein isolation. Rats treated with the 10-mg/kg and 30-mg/kg doses of nicorandil exhibited significantly increased tissue survival in the zone of stasis at days 1 and 3 after injury...
April 12, 2018: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/29748061/massive-ggaas-in-genomic-repetitive-sequences-serve-as-a-nuclear-reservoir-of-nf-%C3%AE%C2%BAb
#10
Jian Wu, Qiao Wang, Wei Dai, Wei Wang, Ming Yue, Jinke Wang
Nuclear factor κB (NF-κB) is a DNA-binding transcription factor. Characterizing its genomic binding sites is crucial for understanding its gene regulatory function and mechanism in cells. This study characterized the binding sites of NF-κB RelA/p65 in the tumor neurosis factor-α (TNFα) stimulated HeLa cells by a precise chromatin immunoprecipitation-sequencing (ChIP-seq). The results revealed that NF-κB binds nontraditional motifs (nt-motifs) containing conserved GGAA quadruplet. Moreover, nt-motifs mainly distribute in the peaks nearby centromeres that contain a larger number of repetitive elements such as satellite, simple repeats and short interspersed nuclear elements (SINEs)...
April 13, 2018: Journal of Genetics and Genomics, Yi Chuan Xue Bao
https://www.readbyqxmd.com/read/29743984/ingredients-from-litsea-garrettii-as-potential-preventive-agents-against-oxidative-insult-and-inflammatory-response
#11
Yan-Ru Li, Guo-Hui Li, Lin Sun, Lin Li, Yue Liu, De-Gang Kong, Shu-Qi Wang, Dong-Mei Ren, Xiao-Ning Wang, Hong-Xiang Lou, Tao Shen
Oxidative stress and inflammation undoubtedly contribute to the pathogenesis of many human diseases. The nuclear transcription factor erythroid 2-related factor (Nrf2) and the nuclear factor κ B (NF- κ B) play central roles in regulation of oxidative stress and inflammation and thus are targets for developing agents against oxidative stress- and inflammation-related diseases. Our previous study indicated that the EtOH extract of Litsea garrettii protected human bronchial epithelial cells against oxidative insult via the activation of Nrf2...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29741292/microrna-150-restores-endothelial-cell-function-and-attenuates-vascular-remodeling-by-targeting-ptx3-through-the-nf-%C3%AE%C2%BAb-signaling-pathway-in-mice-with-acute-coronary-syndrome
#12
Xian-Yuan Luo, Xiao-Qing Zhu, Ying Li, Xue-Bin Wang, Wei Yin, Yi-Shan Ge, Wei-Min Ji
MicroRNAs have been known to function as important regulators in the vascular system, with various physiopathological effects such as vascular remodeling and hypertension modulation. We aimed to explore whether microRNA-150 (miR-150) regulates endothelial cell function and vascular remodeling in acute coronary syndrome (ACS), and the involvement of PTX3 and NF-κB signaling pathway. Ten normal mice and sixty ApoE-/- mice were chosen, and their coronary artery tissues and endothelial cells were extracted. ApoE-/- mice were injected with a series of inhibitors or mimics for miR-150, or siRNA against PTX3...
May 9, 2018: Cell Biology International
https://www.readbyqxmd.com/read/29738082/suppression-of-ikk-but-not-activation-of-p53-is-responsible-for-cell-death-mediated-by-naturally-occurring-oxidized-tetranortriterpenoid
#13
Pankaj Gupta, Adeel H Zaidi, Sunil K Manna
Tetranortriterpenoids (limonoids) obtained from the neem tree (Azadirachta indica) have gained significant attention due to their anti-proliferative properties. Here we are investigating the role of a highly oxidized tetranortriterpenoid, azadirachtin on induction of the cell death. Using various apoptotic assays, we show that azadirachtin induces cell death independent of cell types. Although azadirachtin-treated cells show increased expression of p53, but no phosphorylation of p53 (at Ser15 and Ser46) is detected...
May 8, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29737211/hepatoprotective-effect-of-wedelolactone-against-concanavalin-a-induced-liver-injury-in-mice
#14
Qingqiong Luo, Jieying Ding, Liping Zhu, Fuxiang Chen, Lili Xu
Eclipta prostrata L. is a traditional Chinese herbal medicine that has been used in the treatment of liver diseases. However, its biological mechanisms remain elusive. The current study aimed to investigate the hepatoprotective effect of wedelolactone, a major coumarin ingredient of Eclipta prostrata L., on immune-mediated liver injury. Using the well-established animal model of Concanavalin A (ConA)-induced hepatitis (CIH), we found that pretreatment of mice with wedelolactone markedly reduced both the serum levels of transaminases and the severity of liver damage...
May 8, 2018: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/29734393/orientia-tsutsugamushi-uses-two-ank-effectors-to-modulate-nf-%C3%AE%C2%BAb-p65-nuclear-transport-and-inhibit-nf-%C3%AE%C2%BAb-transcriptional-activation
#15
Sean M Evans, Kyle G Rodino, Haley E Adcox, Jason A Carlyon
Orientia tsutsugamushi causes scrub typhus, a potentially fatal infection that threatens over one billion people. Nuclear translocation of the transcription factor, NF-κB, is the central initiating cellular event in the antimicrobial response. Here, we report that NF-κB p65 nuclear accumulation and NF-κB-dependent transcription are inhibited in O. tsutsugamushi infected HeLa cells and/or primary macrophages, even in the presence of TNFα. The bacterium modulates p65 subcellular localization by neither degrading it nor inhibiting IκBα degradation...
May 7, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29733843/modulation-of-nuclear-factor-%C3%AE%C2%BAb-signaling-and-reduction-of-neural-tube-defects-by-quercetin-3-glucoside-in-embryos-of-diabetic-mice
#16
Chengyu Tan, Fantong Meng, E Albert Reece, Zhiyong Zhao
BACKGROUND: Diabetes mellitus in early pregnancy increases the risk of birth defects in infants. Maternal hyperglycemia stimulates the expression of nitric oxide (NO) synthase 2 (NOS2), which can be regulated by transcription factors of the nuclear factor-κB (NF-κB) family. Increases in reactive nitrogen species (RNS) generate intracellular stress conditions, including nitrosative, oxidative, and endoplasmic reticulum (ER) stresses, and trigger programmed cell death (or apoptosis) in the neural folds, resulting in neural tube defects (NTDs) in the embryo...
May 4, 2018: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29731901/tetrandrine-suppresses-adhesion-migration-and-invasion-of-human-colon-cancer-sw620-cells-via-inhibition-of-nuclear-factor-%C3%AE%C2%BAb-matrix-metalloproteinase-2-and-matrix-metalloproteinase-9-signaling-pathways
#17
Ta-Kuo Juan, Kuo-Ching Liu, Chao-Lin Kuo, Mei-Due Yang, Yung-Lin Chu, Jiun-Long Yang, Ping-Ping Wu, Yi-Ping Huang, Kuang-Chi Lai, Jing-Gung Chung
Tetrandrine (TET) exhibits biological activities, including anticancer activity. In Chinese medicine, TET has been used to treat hypertensive and arrhythmic conditions and has been demonstrated to induce cytotoxic effects on human cancer cell lines. However, to the best of the author's knowledge, no previous studies have revealed that TET affects cell metastasis in SW620 human colon cancer cells. The present study demonstrated that TET decreased the cell number and inhibited cell adhesion and mobility of SW620 cells...
May 2018: Oncology Letters
https://www.readbyqxmd.com/read/29731900/downregulation-of-frizzled-7-induces-the-apoptosis-of-hepatocellular-carcinoma-cells-through-inhibition-of-nf-%C3%AE%C2%BAb
#18
Yuyang Xue, Cong Chen, Wei Xu, Hao Xu, Junnian Zheng, Yuming Gu
The aim of the present study was to investigate the functional role of Frizzled-7 (FZD7) in the apoptosis of hepatoma cells. HepG2 and Huh-7 hepatocellular carcinoma (HCC) cell lines with FZD7 expression were selected for use in the present study. The small hairpin RNA (shRNA) eukaryotic expression vector specific to FZD7 was constructed using gene recombination, and was then transfected into HepG2 and Huh-7 hepatoma cell lines using Lipofectamine 2000 to assess whether the downregulation of FZD7 could affect the proliferative ability of these cells...
May 2018: Oncology Letters
https://www.readbyqxmd.com/read/29731448/epigallocatechin-gallate-inhibits-corneal-neovascularization-in-ratalkaline-burn-model
#19
Huiyan Li, Bei Ye, Lili Zhu, Lianqun Wu
To evaluate the effectiveness of epigallocatechin gallate (EGCG) in inhibiting corneal neovascularization in rat alkaline burn model. Corneal neovascularization model was induced by sodium hydroxide alkaline burn injury in SD rats. Rats were randomly divided into two groups and were given intraperitoneal injection with EGCG or PBS per day for up to 14 days respectively. Corneal inflammation and neovascularization area were assessed on days 3, 7, and 14 after cauterization with digital photographs. Vascular endothelial growth factor (VEGF) and pigment epithelium derived factor (PEDF) mRNA levels were measured by reverse transcription-polymerase chain reaction (qRT-PCR)...
May 2018: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29727715/microrna-326-aggravates-acute-lung-injury-in-septic-shock-by-mediating-the-nf-%C3%AE%C2%BAb-signaling-pathway
#20
Chun-Ting Wu, Yan Huang, Zhen-Ye Pei, Xi Xin, Guang-Fa Zhu
Previously, several previous studies have demonstrated that the activation of the nuclear factor-kappa B (NF-κB) signaling pathway contributes to the development of lipopolysaccharide (LPS)-induced acute lung injury (ALI) as well as an inflammatory reaction, and its inhibition may provide future therapeutic values. Thereby, this currents study aims to explore the effects of miR-326 on inflammatory response and ALI in mice with septic shock via the NF-κB signaling pathway. The study included normal mice and LPS-induced mouse models of septic shock with ALI...
May 1, 2018: International Journal of Biochemistry & Cell Biology
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