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Lin Shen, Yinghua Wu, Liang Han, Haiying Zhang
Low back pain (LBP) is one of the most common musculoskeletal diseases in the world. The incidence is ~70% in adults and many of them suffer from disability. Recently, intervertebral disc degeneration (IDD) has been deemed as a main cause of LBP. The present study aimed to investigate the potentials of growth and differentiation factor-5 (GDF-5) in IDD. The protein levels of prostaglandin-E2 (PGE2), tumor necrosis factor (TNF)-α and interleukin (IL)-1β in culture medium were evaluated by ELISA. mRNA and protein expression levels in nucleus pulposus (NP) cells were evaluated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting, respectively...
April 2018: Experimental and Therapeutic Medicine
Ming Ma, Xingxiao Yang, Lianmei Zhao, Xuexiao Wang, Lihua Liu, Wenjing Jiao, Yuanyuan Wei, Baoen Shan
Celecoxib is a newly-identified nonsteroidal anti-inflammatory drug, which has been used to treat fever in clinical practice. Celecoxib has been demonstrated to suppress the viability of various human tumor cells. However, the effect of celecoxib on response of T-cell lymphoma to chemotherapy agents remains unclear. The aim of the present study was to investigate the effect of celecoxib on chemosensitivity of human T-cell lymphoma, and to address the underlying mechanism of action. The cytotoxicity of CDDP, epirubicin and VCR on Jurkat and Hut-78 cells treated with celecoxib was assessed by MTT assay, and the half-maximal inhibitory concentration (IC50 ) value was calculated by Origin 75 software...
April 2018: Oncology Letters
Meysam Nasiri, Mohammad-Hossein Karimi, Negar Azarpira, Iraj Saadat
OBJECTIVES: Toll-like receptors and downstream signal transduction pathways play pivotal roles in induction of inflammation, which is crucial for liver injury and regeneration. MATERIALS AND METHODS: Using a mouse model of partial hepatic ischemia-reperfusion injury followed by a 28-day time course for liver repair and regeneration, we assessed gene expression levels for Toll-like receptors, myeloid differentiation primary response 88, TIR-domain-containing adapter-inducing interferon-β, nuclear factor κB, interferon regulatory factors, tumor necrosis factor-α, and interleukins 1β and 6 at days 1, 4, 7, 14, and 28 after reperfusion in liver and blood cells by quantitative polymerase chain reaction...
March 9, 2018: Experimental and Clinical Transplantation
Hisashi Kanemaru, Fumihiro Yamane, Hiroki Tanaka, Kazuhiko Maeda, Takashi Satoh, Shizuo Akira
Growing evidence has revealed that the transcription factor basic leucine zipper transcription factor ATF-like 2 (BATF2) has unique transcriptional activities, including regulating cytokines via TLR signals in macrophages, which affect mortality due to infection and cancer. Based on genome-wide analyses using the chromatin immunoprecipitation-sequencing technique, we found that dual-specificity phosphatase 2 (Dusp2) had a significantly lower acetyl-histone status in Batf2-/- bone marrow-derived macrophages (BMDMs) compared with wild-type (WT) BMDMs...
March 9, 2018: International Immunology
Ping Fan, Amit K Tyagi, Fadeke A Agboke, Rohit Mathur, Niranjana Pokharel, V Craig Jordan
Stress responses are critical for estrogen (E2 )-induced apoptosis in E2 -deprived breast cancer cells. Nuclear factor-kappa B (NF-κB) is an important therapeutic target to prevent stress responses in chronic inflammatory diseases including cancer. However, whether E2 activates NF-κB to participate in stress-associated apoptosis in E2 -deprived breast cancer cells is unknown. Here, we demonstrated that E2 differentially modulates NF-κB activity according to treatment time. E2 initially has significant potential to suppress NF-κB activation; it completely blocks tumor necrosis factor alpha (TNFα)-induced activation of NF-κB...
December 2018: Cell Death Discovery
Isabella Faraoni, Francesca Aloisio, Antonio De Gabrieli, Maria Irno Consalvo, Serena Lavorgna, Maria Teresa Voso, Francesco Lo-Coco, Grazia Graziani
Olaparib is a potent orally bioavailable poly(ADP-ribose) polymerase inhibitor (PARPi), approved for BRCA-mutated ovarian and breast cancers. We recently showed that olaparib at clinically achievable concentrations exerts anti-proliferative and pro-apoptotic effects in vitro as monotherapy against primary acute myeloid leukemia (AML) blasts, while sparing normal bone marrow (BM) hematopoietic cells. Since AML expresses low levels of death receptors that may contribute to apoptosis resistance, in this study we investigated whether the anti-leukemia activity of olaparib involves modulation of FAS and TRAIL receptors DR5 and DR4...
March 8, 2018: Cancer Letters
Dong-Mei Wu, Yong-Jian Wang, Xin-Rui Han, Xin Wen, Lei Li, Lan Xu, Jun Lu, Yuan-Lin Zheng
In this study, we aim to investigate the role of tanshinone IIA in myocardial infarction (MI), especially in left ventricular remodelling (VR) and the underlying mechanism involving the TLR4/MyD88/NF-κB signalling pathway. Sprague-Dawley (SD) rats (n = 96) were selected, and 12 of them underwent sham surgery. The remaining 84 rats were subjected to MI modelling. HE and MT staining were carried out to estimate infract size, histopathological changes and fibrosis degree. Macrophage infiltration and cardiomyocyte apoptosis were evaluated by immunohistochemistry and TUNEL staining...
March 10, 2018: Journal of Cellular and Molecular Medicine
Xianyao Li, Yaqin Tang, Binbin Ma, Zheng Wang, Jinying Jiang, Shengjie Hou, Shuhang Wang, Jie Zhang, Meichun Deng, Zhigui Duan, Xing Tang, Alex F Chen, Liping Jiang
OBJECTIVE: The peptide lycosin-I has anti-bacterial and anti-cancer capacities. However, the anti-inflammatory activity of lycosin-I remains unknown. We investigated whether lycosin-I could attenuate inflammation. MATERIALS AND METHODS: Human umbilical vein endothelial cells (HUVECs) were treated with lycosin-I before exposure to tumor necrosis factor-α (TNF-α). The expression of intercellular cell adhesion molecule-1 (ICAM-1), nuclear transcription factor-kappa B (NF-κB) p65 and inhibitory subunit of NF-κB alpha (IκBα) was evaluated by western blot...
March 10, 2018: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
Yuejun Wang, Yunsong Liu, Min Zhang, Longwei Lv, Xiao Zhang, Ping Zhang, Yongsheng Zhou
BACKGROUND: Mesenchymal stem cells (MSCs) are a reliable resource for bone regeneration and tissue engineering, but the molecular mechanisms of differentiation remain unclear. The tumor antigen 15-leucine-rich repeat containing membrane protein (LRRC15) is a transmembrane protein demonstrated to play important roles in cancer. However, little is known about its role in osteogenesis. This study was to evaluate the functions of LRRC15 in osteogenic differentiation of MSCs. METHODS: Osteogenic-induction treatment and the ovariectomized (OVX) model were performed to investigate the potential relationship between LRRC15 and MSC osteogenesis...
March 9, 2018: Stem Cell Research & Therapy
Tzenge-Lien Shih, Ming-Hwa Liu, Chia-Wai Li, Chia-Feng Kuo
A series of B-ring, halo-substituted chalcones and azachalcones were synthesized to evaluate and compare their anti-inflammatory activity. Mouse BALB/c macrophage RAW 264.7 were pre-treated with 10 μg/mL of each compound for one hour before induction of inflammation by lipopolysaccharide (1 μg/mL) for 6 h. Some halo-chalcones and -azachalcones suppressed expression of pro-inflammatory factors toll-like receptor 4 (TLR4), IκB-α, transcription factor p65, interleukine 1β (IL-1β), IL-6, tumor necrosis factor α (TNF-α), and cyclooxygenase 2 (COX-2)...
March 7, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Tugba Soydas, Elif Yaprak Sarac, Suzan Cinar, Sibel Dogan, Seyhun Solakoglu, Matem Tuncdemir, Gonul Kanigur Sultuybek
Metformin is the most widely used anti-diabetic drug in the world. It reduces advanced glycation end product (AGEs)-induced ROS generation in high glucose condition. Protein glycation contributes to skin aging as it deteriorates the existing collagen by crosslinking. The progressive increase of AGE during aging not only causes oxidative damage to cellular macromolecules but also modulates the activation of transcription factors nuclear factor kappa-B(NF-kB). However, it is still unclear whether metformin can change collagen production and NF-kB activity induced by high glucose conditions in 3T3 fibroblast...
March 6, 2018: Journal of Physiology and Biochemistry
Valentina Caracciolo, Jeanette Young, Donna Gonzales, Yinchun Ni, Stephen J Flowers, Ross Summer, Scott A Waldman, Jason K Kim, Dae Young Jung, Hye Lim Noh, Taekyoon Kim, Perry J Blackshear, Danielle O'Connell, Robert C Bauer, Caleb B Kallen
Obesity is associated with adipose tissue inflammation that contributes to insulin resistance. Zinc Finger Protein 36 (Zfp36) is an mRNA-binding protein that reduces inflammation by binding to cytokine transcripts and promoting their degradation. We hypothesized that myeloid-specific deficiency of Zfp36 would lead to increased adipose tissue inflammation and reduced insulin sensitivity in diet-induced obese mice. As expected, wild type (Control) mice became obese and diabetic on a high-fat diet and obese mice with myeloid-specific loss of Zfp36 (KO) demonstrated increased adipose tissue and liver cytokine mRNA expression compared to Control mice...
March 6, 2018: American Journal of Physiology. Endocrinology and Metabolism
Na Liang, Yaxin Sang, Weihua Liu, Wenlong Yu, Xianghong Wang
Gingerol was the main functional substance of Zingiberaceous plant which has been known as traditional medicine for thousands of years. The purpose of this experiment was to explore anti-inflammatory effects of gingerol and study the possible mechanism in lipopolysaccharide (LPS)-stimulated RAW246.7 cells. The cells were treated with 10 μg/mL LPS and 300, 200, 100, and 50 μg/mL gingerol for 24 h. The cytotoxicity of gingerol was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zoliumbromide (MTT) method...
March 5, 2018: Inflammation
Ritu Mishra, Houda Benlhabib, Wei Guo, Connie B Lerma Cervantes, Carole R Mendelson
The major surfactant protein, SP-A (product of SFTPA gene), serves as a marker of type II pneumocyte differentiation and surfactant synthesis. SFTPA expression in cultured human fetal lung (HFL) epithelial cells is upregulated by hormones that increase cAMP and activate TTF-1/NKX2.1 and NF-κB. To further define mechanisms for type II cell differentiation and induction of SP-A, we investigated roles of miRNAs. Using microarray to identify differentially expressed miRNAs in HFL epithelial cells during type II cell differentiation in culture, we observed that members of the miR-199a/-214 cluster were significantly downregulated during differentiation...
March 5, 2018: Molecular and Cellular Biology
Amit Katiyar, Sujata Sharma, Tej P Singh, Punit Kaur
Women with endometriosis (EMS) appear to be at a higher risk of developing other autoimmune diseases predominantly multiple sclerosis (MS). Though EMS and MS are evidently diverse in their phenotype, they are linked by a common autoimmune condition or immunodeficiency which could play a role in the expansion of endometriosis and possibly increase the risk of developing MS in women with EMS. However, the common molecular links connecting EMS with MS are still unclear. We conducted a meta-analysis of microarray experiments focused on EMS and MS with their respective controls...
2018: Frontiers in Genetics
Ru Chen, Yanqiu Yang, Jikai Xu, Yingni Pan, Wenqiang Zhang, Yachao Xing, Hui Ni, Yu Sun, Yue Hou, Ning Li
BACKGROUND: Tamarix species are well known as the main host plants of Herba Cistanches, a valuable Traditional Chinese Medicine. They are also traditional medicinal plants themselves and are used to treat spleen problems, leucoderma and ocular conditions. PURPOSE: The aim of the present study was to investigate the anti-inflammatory effect of Tamarix hohenackeri Bunge. METHODS: In the present study, BV-2 microglial cells were used and stimulated with lipopolysaccharide (LPS)...
February 1, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Jooeun Bae, Teru Hideshima, Yu-Tzu Tai, Yan Song, Paul Richardson, Noopur Raje, Nikhil C Munshi, Kenneth C Anderson
Histone deacetylases (HDAC) are therapeutic targets in multiple cancers. ACY241, an HDAC6 selective inhibitor, has shown anti-multiple myeloma (MM) activity in combination with immunomodulatory drugs and proteasome inhibitors. Here we show ACY241 significantly reduces the frequency of CD138+ MM cells, CD4+ CD25+ FoxP3+ regulatory T cells, and HLA-DRLow/- CD11b+ CD33+ myeloid-derived suppressor cells; and decreases expression of PD1/PD-L1 on CD8+ T cells and of immune checkpoints in bone marrow cells from myeloma patients...
February 22, 2018: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
Linda Xiaoyan Li, Julie Xia Zhou, James P Calvet, Andrew K Godwin, Roy A Jensen, Xiaogang Li
We identified SMYD2, a SMYD (SET and MYND domain) family protein with lysine methyltransferase activity, as a novel breast cancer oncogene. SMYD2 was expressed at significantly higher levels in breast cancer cell lines and in breast tumor tissues. Silencing of SMYD2 by RNAi in triple-negative breast cancer (TNBC) cell lines or inhibition of SMYD2 with its specific inhibitor, AZ505, significantly reduced tumor growth in vivo. SMYD2 executes this activity via methylation and activation of its novel non-histone substrates, including STAT3 and the p65 subunit of NF-κB, leading to increased TNBC cell proliferation and survival...
February 27, 2018: Cell Death & Disease
Zhe Qu, Santosh R D'Mello
A defining feature of neurodegenerative diseases is the abnormal and excessive loss of neurons. One molecule that is particularly important in promoting neuronal death in a variety of cell culture and in vivo models of neurodegeneration is histone deacetylase-3 (HDAC3), a member of the histone deacetylase family of proteins. As a step towards understanding how HDAC3 promotes neuronal death, we conducted a proteomic screen aimed at identifying proteins that were regulated by HDAC3. HDAC3 was overexpressed in cultured rat cerebellar granule neurons (CGNs) and protein lysates were analyzed by mass spectrometry...
January 1, 2018: Experimental Biology and Medicine
Juan Li, Ao Guo, Qinying Wang, Yuanyuan Li, Jian Zhao, Jing Lu, Gang Pei
β-Arrestins (β-arrestin-1 and -2) are multifunctional proteins that play important roles in the regulation of inflammation and cell survival that need to be tightly controlled; however, the mechanism that underlies their gene expression is largely unclear. Here, we demonstrate that β-arrestin-1 is a transcriptional target of NF-κB. mRNA and protein levels of β-arrestin-1 were up-regulated by NF-κB inducers. Inhibition of NF-κB prevented the up-regulation of β-arrestin-1 mRNA, whereas activation of NF-κB led to increased β-arrestin-1 expression...
February 27, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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