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Olivier Sallin, Luc Reymond, Corentin Gondrand, Fabio Raith, Birgit Koch, Kai Johnsson
We introduce a new class of semisynthetic fluorescent biosensors for the quantification of free nicotinamide adenine dinucleotide (NAD+ ) and ratios of reduced to oxidized nicotinamide adenine dinucleotide phosphate (NADPH/NADP+ ) in live cells. Sensing is based on controlling the spatial proximity of two synthetic fluorophores by binding of NAD(P) to the protein component of the sensor. The sensors possess a large dynamic range, can be excited at long wavelengths, are pH-insensitive, have tunable response range and can be localized in different organelles...
May 29, 2018: ELife
Sandra Martin-Brevet, Borja Rodríguez-Herreros, Jared A Nielsen, Clara Moreau, Claudia Modenato, Anne M Maillard, Aurélie Pain, Sonia Richetin, Aia E Jønch, Abid Y Qureshi, Nicole R Zürcher, Philippe Conus, Wendy K Chung, Elliott H Sherr, John E Spiro, Ferath Kherif, Jacques S Beckmann, Nouchine Hadjikhani, Alexandre Reymond, Randy L Buckner, Bogdan Draganski, Sébastien Jacquemont
BACKGROUND: 16p11.2 breakpoint 4 to 5 copy number variants (CNVs) increase the risk for developing autism spectrum disorder, schizophrenia, and language and cognitive impairment. In this multisite study, we aimed to quantify the effect of 16p11.2 CNVs on brain structure. METHODS: Using voxel- and surface-based brain morphometric methods, we analyzed structural magnetic resonance imaging collected at seven sites from 78 individuals with a deletion, 71 individuals with a duplication, and 212 individuals without a CNV...
March 27, 2018: Biological Psychiatry
Thissa Siriwardena, Alice Capecchi, Bee-Ha Gan, Xian Jin, Runze He, Dengwen Wei, Lan Ma, Thilo Köhler, Christian van Delden, Sacha Javor, Jean-Louis Reymond
Here we used nearest neighbor searches in chemical space to improve the activity of antimicrobial peptide dendrimer (AMPD) G3KL and identified dendrimer T7 with an expanded activity range against Gram-negative pathogenic bacteria including Klebsiellae pneumoniae, increased serum stability and promising activity in an in vivo infection model against a multidrug resistant strain of Acinetobacter baumannii. Imaging, spectroscopic studies and a structural model from molecular dynamics simulations suggest that T7 acts by membrane disruption...
May 16, 2018: Angewandte Chemie
Sue Healy, Fiona Israel, Margaret Charles, Liz Reymond
BACKGROUND: Palliative care patients consistently nominate home as their preferred care environment. This is challenging without support from laycarers, especially if patients require subcutaneously administered symptom relief. Laycarers typically lack confidence with this task and request professional guidance. AIM: To explore differences in laycarers' confidence in administering subcutaneous injections depending upon whether a laycarer, registered nurse or pharmacist prepared injections for subsequent administration by laycarers...
May 1, 2018: Palliative Medicine
Ivan Di Bonaventura, Stéphane Baeriswyl, Alice Capecchi, Bee-Ha Gan, Xian Jin, Thissa N Siriwardena, Runze He, Thilo Köhler, Arianna Pompilio, Giovanni Di Bonaventura, Christian van Delden, Sacha Javor, Jean-Louis Reymond
We used the concept of chemical space to explore a virtual library of bicyclic peptides formed by double thioether cyclization of a precursor linear peptide, and identified an antimicrobial bicyclic peptide (AMBP) with remarkable activity against several MDR strains of Acinetobacter baumannii and Pseudomonas aeruginosa.
May 2, 2018: Chemical Communications: Chem Comm
Rahul Dhandapani, Cynthia Mary Arokiaraj, Francisco J Taberner, Paola Pacifico, Sruthi Raja, Linda Nocchi, Carla Portulano, Federica Franciosa, Mariano Maffei, Ahmad Fawzi Hussain, Fernanda de Castro Reis, Luc Reymond, Emerald Perlas, Simone Garcovich, Stefan Barth, Kai Johnsson, Stefan G Lechner, Paul A Heppenstall
Mechanical allodynia is a major symptom of neuropathic pain whereby innocuous touch evokes severe pain. Here we identify a population of peripheral sensory neurons expressing TrkB that are both necessary and sufficient for producing pain from light touch after nerve injury in mice. Mice in which TrkB-Cre-expressing neurons are ablated are less sensitive to the lightest touch under basal conditions, and fail to develop mechanical allodynia in a model of neuropathic pain. Moreover, selective optogenetic activation of these neurons after nerve injury evokes marked nociceptive behavior...
April 24, 2018: Nature Communications
F El Hage, D Legland, N Borrega, M-P Jacquemot, Y Griveau, S Coursol, V Méchin, M Reymond
Water supply and valorization are two urgent issues in the utilization of maize biomass in the context of climate change and replacement of fossil resources. Maximizing maize biomass valorization is of interest to make biofuel conversion competitive, and to increase forage energetic value for animal fodder. One way to estimate biomass valorization is to quantify cell wall degradability. In this study, we evaluated the impact of water supply on cell wall degradability, cell wall contents and structure, and distribution of lignified cell types in maize internodes using dedicated high-throughput tools to effectively phenotype maize internodes from 11 inbred lines under two contrasting irrigation scenarios in field trials over three years...
May 16, 2018: Journal of Agricultural and Food Chemistry
RudÁ Amorim Lucena, Martin Lindsey Christoffersen
The Brazilian coast is over 7000 km long. It is comprised of diverse environments such as reef, lagoons and mangroves. These diverse habitats support great biological diversity but, due to the extension of the coast, knowledge of the pycnogonid fauna is poor and fragmented. Records from Brazil began with Krøyer in the 1840s. Collections from Brazilian waters by the "H.M.S. Challenger" and "Vettor Pisani" expeditions were published by Hoek (1881) and Schimkewitsch (1888). For almost 50 years there were no further publications, until Marcus (1940c) published his comprehensive paper on the South American fauna...
January 9, 2018: Zootaxa
Alexander Kettner, Gilles DellaCorte, Fréderic de Blay, Lars Jacobsen, Marek Jutel, Margitta Worm, Vincent Charlon, Kim Simonsen, Christophe Reymond, François Spertini
No abstract text is available yet for this article.
April 17, 2018: Journal of Allergy and Clinical Immunology
F X Reymond Sutandy, Stefanie Ebersberger, Lu Huang, Anke Busch, Maximilian Bach, Hyun-Seo Kang, Jörg Fallmann, Daniel Maticzka, Rolf Backofen, Peter F Stadler, Kathi Zarnack, Michael Sattler, Stefan Legewie, Julian König
Alternative splicing generates distinct mRNA isoforms and is crucial for proteome diversity in eukaryotes. The RNA-binding protein (RBP) U2AF2 is central to splicing decisions, as it recognizes 3' splice sites and recruits the spliceosome. We establish "in vitro iCLIP" experiments, in which recombinant RBPs are incubated with long transcripts, to study how U2AF2 recognizes RNA sequences and how this is modulated by trans -acting RBPs. We measure U2AF2 affinities at hundreds of binding sites and compare in vitro and in vivo binding landscapes by mathematical modeling...
May 2018: Genome Research
Lijo Cherian Ozhathil, Clémence Delalande, Beatrice Bianchi, Gabor Nemeth, Sven Kappel, Urs Thomet, Daniela Ross-Kaschitza, Céline Simonin, Matthias Rubin, Jürg Gertsch, Martin Lochner, Christine Peinelt, Jean-Louis Reymond, Hugues Abriel
BACKGROUND AND PURPOSE: TRPM4 is a calcium-activated non-selective cation channel expressed in many tissues and implicated in several diseases, and has not yet been validated as a therapeutic target due to the lack of potent and selective inhibitors. We sought to discover a novel series of small-molecule inhibitors by combining in silico methods and cell-based screening assay, with sub-micromolar potency and improved selectivity from previously reported TRPM4 inhibitors. EXPERIMENTAL APPROACH: Here, we developed a high throughput screening compatible assay to record TRPM4-mediated Na+ influx in cells using a Na+ -sensitive dye and used this assay to screen a small set of compounds selected by ligand-based virtual screening using previously known weakly active and non-selective TRPM4 inhibitors as seed molecules...
March 26, 2018: British Journal of Pharmacology
Caragh Whitehead, Francisco J Ostos Garrido, Matthieu Reymond, Rachael Simister, Assaf Distelfeld, Sergio G Atienza, Fernando Piston, Leonardo D Gomez, Simon J McQueen-Mason
The recalcitrance of secondary plant cell walls to digestion constrains biomass use for the production of sustainable bioproducts and for animal feed. We screened a population of Brachypodium recombinant inbred lines (RILs) for cell wall digestibility using commercial cellulases and detected a quantitative trait locus (QTL) associated with this trait. Examination of the chromosomal region associated with this QTL revealed a candidate gene that encodes a putative glycosyl transferase family (GT) 43 protein, orthologue of IRX14 in Arabidopsis, and hence predicted to be involved in the biosynthesis of xylan...
May 2018: New Phytologist
Maciej Nowacki, Mohammad Alyami, Laurent Villeneuve, Frederic Mercier, Martin Hubner, Wouter Willaert, Wim Ceelen, Marc Reymond, Denis Pezet, Catherine Arvieux, Vladimir Khomyakov, Laura Lay, Sergio Gianni, Wojciech Zegarski, Naoual Bakrin, Olivier Glehen
BACKGROUND: Pressurized intraperitoneal aerosol chemotherapy (PIPAC) is a new drug delivery method offered in selected patients suffering from non-resectable peritoneal carcinomatosis (PC). As reported experience is still limited, we conducted a survey among active PIPAC centers aiming to report their technical approach and clinical findings. METHODS: An online survey was sent to active PIPAC centers worldwide. The questionnaire consisted of 34 closed questions and was conducted over a period of 3 months beginning in March 2017...
February 22, 2018: European Journal of Surgical Oncology
(no author information available yet)
No abstract text is available yet for this article.
April 2018: Plant Physiology
Julio Dueñas, James Sulzer, Philipp Stämpfli, Marie-Claude Hepp-Reymond, Spyros Kollias, Erich Seifritz, Roger Gassert
Peripheral encoding of movement kinematics has been well-characterized, but there is little understanding of the relationship between movement kinematics and associated brain activation. We hypothesized that kinematics of passive movement is differentially represented in the sensorimotor network, reflecting the well-studied afferent responses to movement. A robotic forefinger manipulandum was used to induce passive kinematic stimuli and monitor interaction force in 41 healthy participants during whole-brain functional magnetic resonance imaging (fMRI)...
June 2018: NeuroImage
Josep Arús-Pous, Daniel Probst, Jean-Louis Reymond
No abstract text is available yet for this article.
February 1, 2018: Chimia
J G Cohen, E Reymond, M Medici, M Lederlin, S Lantuejoul, F Laurent, A C Toffart, A Moreau-Gaudry, A Jankowski, G R Ferretti
PURPOSE: The purpose of this study was to evaluate the usefulness of computed tomography-texture analysis (CTTA) in differentiating between in-situ and minimally-invasive from invasive adenocarcinomas in subsolid lung nodules (SSLNs). MATERIAL AND METHODS: Two radiologists retrospectively reviewed 49 SSLNs in 44 patients. There were 27 men and 17 women with a mean age of 63±7 (SD) years (range: 47-78years). For each SSLN, type (pure ground-glass or part-solid) was assessed by consensus and CTTA was conducted independently by each observer using a filtration-histogram technique...
May 2018: Diagnostic and Interventional Imaging
Marleen Stuhr, Bernhard Blank-Landeshammer, Claire E Reymond, Laxmikanth Kollipara, Albert Sickmann, Michal Kucera, Hildegard Westphal
The proliferation of key marine ecological engineers and carbonate producers often relies on their association with photosymbiotic algae. Evaluating stress responses of these organisms is important to predict their fate under future climate projections. Physiological approaches are limited in their ability to resolve the involved molecular mechanisms and attribute stress effects to the host or symbiont, while probing and partitioning of proteins cannot be applied in organisms where the host and symbiont are small and cannot be physically separated...
February 23, 2018: Scientific Reports
Pierre Baillet, Guillaume Cadiot, Marion Goutte, Felix Goutorbe, Hedia Brixi, Christine Hoeffel, Christophe Allimant, Maud Reymond, Hélène Obritin-Guilhen, Benoit Magnin, Gilles Bommelaer, Bruno Pereira, Constance Hordonneau, Anthony Buisson
AIM: To assess magnetic resonance imaging (MRI) and faecal calprotectin to detect endoscopic postoperative recurrence in patients with Crohn's disease (CD). METHODS: From two tertiary centers, all patients with CD who underwent ileocolonic resection were consecutively and prospectively included. All the patients underwent MRI and endoscopy within the first year after surgery or after the restoration of intestinal continuity [median = 6 mo (5.0-9.3)]. The stools were collected the day before the colonoscopy to evaluate faecal calprotectin level...
February 7, 2018: World Journal of Gastroenterology: WJG
Ana S Ramírez, Jérémy Boilevin, Ahmad Reza Mehdipour, Gerhard Hummer, Tamis Darbre, Jean-Louis Reymond, Kaspar P Locher
The membrane-associated, processive and retaining glycosyltransferase PglH from Campylobacter jejuni is part of the biosynthetic pathway of the lipid-linked oligosaccharide (LLO) that serves as the glycan donor in bacterial protein N-glycosylation. Using an unknown counting mechanism, PglH catalyzes the transfer of exactly three α1,4 N-acetylgalactosamine (GalNAc) units to the growing LLO precursor, GalNAc-α1,4-GalNAc-α1,3-Bac-α1-PP-undecaprenyl. Here, we present crystal structures of PglH in three distinct states, including a binary complex with UDP-GalNAc and two ternary complexes containing a chemo-enzymatically generated LLO analog and either UDP or synthetic, nonhydrolyzable UDP-CH2 -GalNAc...
January 31, 2018: Nature Communications
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