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JOURNAL ARTICLE
Alicia A Lork, Stefania Rabasco, Carl Ernst, André du Toit, Silvio O Rizzoli, Nhu T N Phan
Protein turnover is a critical process for accurate cellular function, in which damaged proteins in the cells are gradually replaced with newly synthesized ones. Many previous studies on cellular protein turnover have used stable isotopic labelling by amino acids in cell culture (SILAC), followed by proteomic bulk analysis. However, this approach does not take into account the heterogeneity observed at the single-cell and subcellular levels. To address this, we investigated the protein turnover of neural progenitor cells at the subcellular resolution, using correlative TEM and NanoSIMS imaging, relying on a pulse-chase analysis of isotopically-labelled protein precusors...
February 28, 2024: Chemical Science
#2
JOURNAL ARTICLE
Emmanuel Berlin, Alicia A Lork, Martin Bornecrantz, Carl Ernst, Nhu T N Phan
Membrane lipids have been known to influence multiple signalling and cellular processes. Dysregulation of lipids at the neuronal membrane is connected to a significant alteration of the brain function and morphology, leading to brain diseases and neurodegeneration. Understanding the lipid composition and turnover of neuronal membrane will provide a significant insight into the molecular events underlying the regulatory effects of these biomolecules in a neuronal system. In this study, we aimed to characterize the composition and turnover of the plasma membrane lipids in human neural progenitor cells (NPCs) at an early differentiation stage into midbrain neurons using ToF-SIMS imaging...
February 14, 2024: Talanta
#3
JOURNAL ARTICLE
Nora Tabea Sibert, Tobias Kurth, Clara Breidenbach, Simone Wesselmann, Günther Feick, Ernst-Günter Carl, Sebastian Dieng, Mohamad Hatem Albarghouth, Atiqullah Aziz, Stefan Baltes, Elisabeth Bartolf, Jens Bedke, Andreas Blana, Marko Brock, Stefan Conrad, Christopher Darr, Florian Distler, Konstantinos Drosos, Gregor Duwe, Amr Gaber, Markus Giessing, Nina Natascha Harke, Axel Heidenreich, Sameh Hijazi, Andreas Hinkel, Björn Theodor Kaftan, Shatlyk Kheiderov, Thomas Knoll, Gerd Lümmen, Inga Peters, Bülent Polat, Valentin Schrodi, Jens-Uwe Stolzenburg, Zoltan Varga, Julius von Süßkind-Schwendi, Vahudin Zugor, Christoph Kowalski
BACKGROUND: Incontinence and sexual dysfunction are long-lasting side effects after surgical treatment (radical prostatectomy, RP) of prostate cancer (PC). For an informed treatment decision, physicians and patients should discuss expected impairments. Therefore, this paper firstly aims to develop and validate prognostic models that predict incontinence and sexual function of PC patients one year after RP and secondly to provide an online decision making tool. METHODS: Observational cohorts of PC patients treated between July 2016 and March 2021 in Germany were used...
2023: PloS One
#4
JOURNAL ARTICLE
Florian G Scurt, Angela Ernst, Carl-Ludwig FischerFröhlich, Anke Schwarz, Jan U Becker, Christos Chatzikyrkou
Several scores have been devised for providing a prognosis of outcomes after kidney transplantation. This study is a comprehensive test of these scores in a cohort of deceased donors with kidneys of lower-than-average quality and procurement biopsies. In total, 15 scores were tested on a retrospective cohort consisting of 221 donors, 223 procurement biopsies, and 223 recipient records for performance on delayed graft function, graft function, or death-censored graft loss. The best-performing score for DGF was the purely clinical Chapal score (AUC 0...
2023: Transplant International
#5
JOURNAL ARTICLE
Florian G Scurt, Carl-Ludwig Fischer-Fröhlich, Tamara Wassermann, Angela Ernst, Anke Schwarz, Jan U Becker, Christos Chatzikyrkou
BACKGROUND: Despite organ shortages, the discard rate of deceased donor kidneys is high. Risk factors for this trend warrant further study. METHODS: We investigated reasons for discard in a cohort of brain death donors with marginal kidneys and procurement biopsies. Paraffin embedded procurement biopsies were systematically reevaluated and graded for the purpose of the study. Assessment included percentage of global glomerulosclerosis, Banff Lesion scores and tubular epithelial damage...
October 19, 2023: Journal of Nephrology
#6
JOURNAL ARTICLE
Livia Parodi, Mary E Comeau, Marios K Georgakis, Ernst Mayerhofer, Jaeyoon Chung, Guido J Falcone, Rainer Malik, Stacie L Demel, Bradford B Worrall, Sebastian Koch, Fernando D Testai, Steven J Kittner, Jacob L McCauley, Christiana E Hall, Douglas J Mayson, Mitchell Sv Elkind, Michael L James, Daniel Woo, Jonathan Rosand, Carl D Langefeld, Christopher D Anderson
OBJECTIVE: Genome-wide association studies have identified 1q22 as a susceptibility locus for cerebral small vessel diseases (CSVDs), including non-lobar intracerebral hemorrhage (ICH) and lacunar stroke. In the present study we performed targeted high-depth sequencing of 1q22 in ICH cases and controls to further characterize this locus and prioritize potential causal mechanisms, which remain unknown. METHODS: 95,000 base pairs spanning 1q22, including SEMA4A, SLC25A44 and PMF1/PMF1-BGLAP were sequenced in 1,055 spontaneous ICH cases (534 lobar and 521 non-lobar) and 1,078 controls...
October 3, 2023: Annals of Neurology
#7
JOURNAL ARTICLE
Christoph Kowalski, Nora Tabea Sibert, Peter Hammerer, Simone Wesselmann, Günter Feick, Ernst-Günther Carl, Theodor Klotz, Hendrik Apel, Sebastian Dieng, Joanne Nyarangi-Dix, Thomas Knoll, Moritz Johannes Reike, Gregor Duwe, Elisabeth Bartolf, Thomas Steiner, Rainer Borowitz, Gerd Lümmen, Anna Katharina Seitz, Jesco Pfitzenmaier, Atiqullah Aziz, Marko Brock, Frank Peter Berger, Björn Theodor Kaftan, Christian Grube, Tim Häfner, Amir Hamza, Hans Schmelz, Jürgen Haas, Sebastian Lenart, Anselm Lafita, Carsten Sippel, Alexander Winter, George Kedia, Boris Hadaschik, Zoltan Varga, Stephan Buse, Matthias Richter, Florian Distler, Jörg Simon, Thomas Wiegel, Stefan Baltes, Andreas Janitzky, Jens Peter Sommer, Sameh Hijazi, Philipp Fülkell, Nina N Harke, Christian Bolenz, Christian Khalil, Clara Breidenbach, Pierre Tennstedt, Martin Burchardt
BACKGROUND: In addition to erectile dysfunction, urinary incontinence is the most common functional limitation after radical prostatectomy (RPE) for prostate cancer (PCa). The German S3 guideline recommends informing patients about possible effects of the therapy options, including incontinence. However, only little data on continence from routine care in German-speaking countries after RPE are currently available, which makes it difficult to inform patients. OBJECTIVE: The aim of this work is to present data on the frequency and severity of urinary incontinence after RPE from routine care...
September 25, 2023: Urologie
#8
JOURNAL ARTICLE
Florian F Hildenbrand, Soenke Boettger, Tobias Spiller, Maria Schubert, Jutta Ernst, Roland von Känel, Carl M Zipser
Delirium incidence and phenotype differ between sexes. Sex differences in the selection of treatment strategies remain elusive. We evaluated sex-specific responses to non- and pharmacological management. In this observational prospective cohort study conducted at the University Hospital Zurich, Switzerland, 602 patients managed for delirium were analyzed. Remission and benefit ratios of treatments were calculated using Cox regression models. Baseline characteristics were similar in both sexes. Overall, 89% of all patients (540/602) received pharmacological management for delirium, most (77%) with one or two different medications...
June 12, 2023: International Clinical Psychopharmacology
#9
REVIEW
Livia Parodi, Ernst Mayerhofer, Kaavya Narasimhalu, Nirupama Yechoor, Mary E Comeau, Jonathan Rosand, Carl D Langefeld, Christopher D Anderson
Cerebral small vessel disease is highly prevalent, particularly in marginalized communities, and its incidence is expected to increase given the aging global population. Cerebral small vessel disease contributes to risk for stroke, vascular cognitive impairment and dementia, late-life depression, and gait disorders. A growing body of evidence suggests that adverse outcomes, including cerebral small vessel disease, caused by traditional cardiovascular risk factors are at least partly mediated by epigenetic changes, some of them already beginning during fetal development...
July 4, 2023: Journal of the American Heart Association
#10
REVIEW
Maie Toomsalu
Today, the University of Tartu (earlier Dorpat) belongs among the 250 best universities of the world. Its international team of pharmacologists uses powerful confocal microscopes to study apoptosis and cell death within an international consortium. Science is working on solutions to fight Alzheimer disease, which is a torture for humankind. For this to happen today, the foundation was laid by scientists of previous centuries who deserve our great respect, all of them together and everyone separately. Johannes Piiper, a well-known professor of physiology, once told me in a conversation that articles should be published in every 10 years about the men who have served as examples for the science of the present-day world and about the conditions in which their research was done...
June 9, 2023: Naunyn-Schmiedeberg's Archives of Pharmacology
#11
JOURNAL ARTICLE
Lionne D F Venderbos, Sebastiaan Remmers, André Deschamps, John Dowling, Ernst-Günter Carl, Nuno Pereira-Azevedo, Monique J Roobol
BACKGROUND: To further strengthen the voice of patients, Europa Uomo initiated the Europa Uomo Patient Reported Outcome Study 2.0 (EUPROMS 2.0) in October 2021. OBJECTIVE: To collect the self-reported perspective of prostate cancer (PCa) patients on physical and mental well-being after PCa treatment outside a clinical trial setting to inform future fellow patients about the impact of PCa treatment. DESIGN, SETTING, AND PARTICIPANTS: Europa Uomo invited PCa patients to complete a cross-sectional survey including the validated EQ-5D-5L, EORTC-QLQ-C30, and the EPIC-26 questionnaires...
May 31, 2023: European Urology Focus
#12
JOURNAL ARTICLE
Nuwan C Hettige, Peter Fleming, Amelia Semenak, Xin Zhang, Huashan Peng, Marc-Daniel Hagel, Jean-François Théroux, Ying Zhang, Anjie Ni, Malvin Jefri, Lilit Antonyan, Shaima Alsuwaidi, Andreas Schuppert, Patrick S Stumpf, Carl Ernst
FOXG1 is a critical transcription factor in human brain where loss-of-function mutations cause a severe neurodevelopmental disorder, while increased FOXG1 expression is frequently observed in glioblastoma. FOXG1 is an inhibitor of cell patterning and an activator of cell proliferation in chordate model organisms but different mechanisms have been proposed as to how this occurs. To identify genomic targets of FOXG1 in human neural progenitor cells (NPCs), we engineered a cleavable reporter construct in endogenous FOXG1 and performed chromatin immunoprecipitation (ChIP) sequencing...
May 22, 2023: Human Molecular Genetics
#13
Livia Parodi, Mary E Comeau, Marios K Georgakis, Ernst Mayerhofer, Jaeyoon Chung, Guido J Falcone, Rainer Malik, Stacie L Demel, Bradford B Worrall, Sebastian Koch, Fernando D Testai, Steven J Kittner, Jacob L McCauley, Christiana E Hall, Douglas J Mayson, Mitchell Sv Elkind, Michael L James, Daniel Woo, Jonathan Rosand, Carl D Langefeld, Christopher D Anderson
OBJECTIVE: Genome-wide association studies have identified 1q22 as a susceptibility locus for cerebral small vessel diseases (CSVDs), including non-lobar intracerebral hemorrhage (ICH) and lacunar stroke. In the present study we performed targeted high-depth sequencing of 1q22 in ICH cases and controls to further characterize this locus and prioritize potential causal mechanisms, which remain unknown. METHODS: 95,000 base pairs spanning 1q22 , including SEMA4A, SLC25A44 and PMF1 / PMF1-BGLAP were sequenced in 1,055 spontaneous ICH cases (534 lobar and 521 non-lobar) and 1,078 controls...
April 24, 2023: medRxiv
#14
JOURNAL ARTICLE
Anna Hinze, Jenny Rinke, Carl C Crodel, Susanne Möbius, Vivien Schäfer, Florian H Heidel, Andreas Hochhaus, Thomas Ernst
Classical myeloproliferative neoplasms (MPNs) are characterized by distinct clinical phenotypes. The discovery of driver mutations in JAK2, CALR and MPL genes provided new insights into their pathogenesis. Next-generation sequencing (NGS) identified additional somatic mutations, most frequently in epigenetic modulator genes. In this study, a cohort of 95 MPN patients was genetically characterized using targeted NGS. Clonal hierarchies of detected mutations were subsequently analysed using colony forming progenitor assays derived from single cells to study mutation acquisition...
July 2023: British Journal of Haematology
#15
JOURNAL ARTICLE
Ulrich Koehler
Johannes Müller was indisputably the most versatile and brilliant physiologist in the mid-nineteenth century. Müller was born in Koblenz in 1801 as the eldest of five children. He received an excellent education in mathematics and the ancient languages and was thus able to read with ease the writings of Aristotle in the original.He served a year with the Pioneers after graduating from high school in 1818. In 1819 he enrolled at the University of Bonn. In 1821, while still a student, he was awarded the scientific university prize for his work on foetal respiration...
April 18, 2023: Pneumologie
#16
JOURNAL ARTICLE
Jung-Ching Liu, Rémy Pawlak, Xing Wang, Hongyan Chen, Philipp D'Astolfo, Carl Drechsel, Ping Zhou, Robert Häner, Silvio Decurtins, Ulrich Aschauer, Shi-Xia Liu, Wulf Wulfhekel, Ernst Meyer
Obtaining a robust superconducting state in atomically precise nanographene (NG) structures by proximity to a superconductor could foster the discovery of topological superconductivity in graphene. On-surface synthesis of such NGs has been achieved on noble metals and metal oxides; however, it is still absent on superconductors. Here, we present a synthetic method to induce superconductivity of polymeric chains and NGs adsorbed on the superconducting Nb(110) substrate covered by thin Ag films. Using atomic force microscopy at low temperature, we characterize the chemical structure of each subproduct formed on the superconducting Ag layer...
April 3, 2023: ACS materials letters
#17
JOURNAL ARTICLE
Andrew J Sawyer, Ellis Patrick, Jarem Edwards, James S Wilmott, Timothy Fielder, Qianting Yang, Daniel L Barber, Joel D Ernst, Warwick J Britton, Umaimainthan Palendira, Xinchun Chen, Carl G Feng
The hallmark of tuberculosis (TB) is the formation of immune cell-enriched aggregates called granulomas. While granulomas are pathologically diverse, their tissue-wide heterogeneity has not been spatially resolved at the single-cell level in human tissues. By spatially mapping individual immune cells in every lesion across entire tissue sections, we report that in addition to necrotizing granulomas, the human TB lung contains abundant non-necrotizing leukocyte aggregates surrounding areas of necrotizing tissue...
June 5, 2023: Journal of Experimental Medicine
#18
MULTICENTER STUDY
Frank Stegelmann, Lino L Teichmann, Florian H Heidel, Carl C Crodel, Thomas Ernst, Sebastian Kreil, Andreas Reiter, Sara Otten, Stefanie Schauer, Ruth-Miriam Körber, Kim Kricheldorf, Susanne Isfort, Hartmut Döhner, Tim H Brümmendorf, Martin Griesshammer, Konstanze Döhner, Steffen Koschmieder
No abstract text is available yet for this article.
April 2023: Leukemia
#19
JOURNAL ARTICLE
Stefania Rabasco, Alicia A Lork, Emmanuel Berlin, Tho D K Nguyen, Carl Ernst, Nicolas Locker, Andrew G Ewing, Nhu T N Phan
Stress granules (SGs) are stress-induced biomolecular condensates which originate primarily from inactivated RNA translation machinery and translation initiation factors. SG formation is an important defensive mechanism for cell survival, while its dysfunction has been linked to neurodegenerative diseases. However, the molecular mechanisms of SG assembly and disassembly, as well as their impacts on cellular recovery, are not fully understood. More thorough investigations into the molecular dynamics of SG pathways are required to understand the pathophysiological roles of SGs in cellular systems...
January 29, 2023: International Journal of Molecular Sciences
#20
JOURNAL ARTICLE
Holger Steinberg
For some years now, the incidence of syphilis and neuroluetic clinical pictures has been increasing. As a result, tabes dorsalis and progressive paralysis are once again gaining relevance in neurology. In the last two decades of the 19th century, there was a heated debate in German neurology about the understanding of the aetiology of these conditions, the so-called 'tabes syphilis controversy'. In 1879, Wilhelm Erb had called on the German neurological community in a much-noticed lecture to finally tackle the problem to unequivocally identify the cause of tabes dorsalis...
January 4, 2023: Fortschritte der Neurologie-Psychiatrie
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