Read by QxMD icon Read


Marcus Geissler, Jascha Volk, Felix Stehle, Oliver Kayser, Heribert Warzecha
OBJECTIVE: Through heterologous expression of the tetrahydrocannabinolic acid synthase (THCAS) coding sequence from Cannabis sativa L. in Nicotiana benthamiana, we evaluated a transient plant-based expression system for the production of enzymes involved in cannabinoid biosynthesis. RESULTS: Thcas was modularized according to the GoldenBraid grammar and its expression tested upon alternative subcellular localization of the encoded catalyst with and without fusion to a fluorescent protein...
April 4, 2018: Biotechnology Letters
Jeremy R Etzkorn, Scott D Tuttle, Ilya Lim, Elea M Feit, Joseph F Sobanko, Thuzar M Shin, Donald E Neal, Christopher J Miller
BACKGROUND: Surgical treatment options for facial melanomas include conventional excision with postoperative margin assessment, Mohs micrographic surgery (MMS) with immunostains (MMS-I), and slow MMS. Patient preferences for these surgical options have not been studied. OBJECTIVES: To evaluate patient preferences for surgical treatment of facial melanoma and to determine how patients value the relative importance of different surgical attributes. METHODS: Participants completed a 2-part study consisting of a stated preference survey and a choice-based conjoint analysis experiment...
March 2, 2018: Journal of the American Academy of Dermatology
Valerie Hauch, Siegfried Ludwig Sporer, Jaume Masip, Iris Blandón-Gitlin
This meta-analysis synthesizes research on interrater reliability of Criteria-Based Content Analysis (CBCA). CBCA is an important component of Statement Validity Assessment (SVA), a forensic procedure used in many countries to evaluate whether statements (e.g., of sexual abuse) are based on experienced or fabricated events. CBCA contains 19 verbal content criteria, which are frequently adapted for research on detecting deception. A total of k = 82 hypothesis tests revealed acceptable interrater reliabilities for most CBCA criteria, as measured with various indices (except Cohen's kappa)...
June 2017: Psychological Assessment
Jorun Sivertsen, Birgitte Graverholt, Birgitte Espehaug
BACKGROUND: Dysphagia is common after stroke and represents a major risk factor for developing aspiration pneumonia. Early detection can reduce the risk of pulmonary complications and death. Despite the fact that evidence-based guidelines recommend screening for swallowing deficit using a standardized screening tool, national audits has identified a gap between practice and this recommendation. The aim was to determine the level of adherence to an evidence-based recommendation on swallow assessment and to take actions to improve practice if necessary...
2017: BMC Nursing
Anna Szabelska, Marcin R Tatara, Witold Krupski
BACKGROUND: Among bones building the axial and appendicular skeleton, the mandible is characterized by unique morphological and functional traits. The aim of the study was to evaluate morphological, densitometric and mechanical properties of mandible in 5-month-old Polish Merino sheep. Using quantitative computed tomography, volumetric bone mineral density (vBMD) and calcium hydroxyapatite density of the cortical bone (CbCa-HA), mean vBMD (MvBMD) and total bone volume were determined...
January 5, 2017: BMC Veterinary Research
Ida Welle, Michel Berclaz, Marie-Josée Lacasa, Gérard Niveau
The growing awareness of sexually-abused children has led to a major shift: previously considered untrustworthy, children are now regarded as competent in providing medico-legal evidence. Professionals undertaking the challenging task of assessing the child's credibility need to rely upon approved evaluation methods. The Criteria-Based Content Analysis is a tool developed to assess the truthfulness of a child's verbal statement. This field-based study explores its validity and its limitations. Three independent experts rated the verbatim statements of 60 real-life alleged victims of sexual abuse...
October 2016: Journal of Forensic and Legal Medicine
Glynis Bogaard, Ewout H Meijer, Aldert Vrij, Harald Merckelbach
The present study investigated the beliefs of students and police officers about cues to deception. A total of 95 police officers and 104 undergraduate students filled out a questionnaire addressing beliefs about cues to deception. Twenty-eight verbal cues were included in the questionnaire, all extracted from verbal credibility assessment tools (i.e., CBCA, RM, and SCAN). We investigated to what extent beliefs about nonverbal and verbal cues of deception differed between lay people (students) and police officers, and whether these beliefs were in agreement with objective cues known from research...
2016: PloS One
Verena A Oberlader, Christoph Naefgen, Judith Koppehele-Gossel, Laura Quinten, Rainer Banse, Alexander F Schmidt
Within the scope of judicial decisions, approaches to distinguish between true and fabricated statements have been of particular importance since ancient times. Although methods focusing on "prototypical" deceptive behavior (e.g., psychophysiological phenomena, nonverbal cues) have largely been rejected with regard to validity, content-based techniques constitute a promising approach and are well established within the applied forensic context. The basic idea of this approach is that experience-based and nonexperience-based statements differ in their content-related quality...
August 2016: Law and Human Behavior
Gérard Niveau, Marie-Josée Lacasa, Michel Berclaz, Michèle Germond
The evaluation of children's statements of sexual abuse cases in forensic cases is critically important and must and reliable. Criteria-based content analysis (CBCA) is the main component of the statement validity assessment (SVA), which is the most frequently used approach in this setting. This study investigated the inter-rater reliability (IRR) of CBCA in a forensic context. Three independent raters evaluated the transcripts of 95 statements of sexual abuse. IRR was calculated for each criterion, total score, and overall evaluation...
September 2015: Journal of Forensic Sciences
Janet H Sanders, Tedd Karr
PURPOSE: Lean and Six Sigma are continuous improvement methodologies that have garnered international fame for improving manufacturing and service processes. Increasingly these methodologies are demonstrating their power to also improve healthcare processes. The purpose of this paper is to discuss a case study for the application of Lean and Six Sigma tools in the reduction of turnaround time (TAT) for Emergency Department (ED) specimens. This application of the scientific methodologies uncovered opportunities to improve the entire ED to lab system for the specimens...
2015: International Journal of Health Care Quality Assurance
Da-Wei Li, Dan Meng, Rafael Brüschweiler
A robust NMR resonance assignment method is introduced for proteins whose 3D structure has previously been determined by X-ray crystallography. The goal of the method is to obtain a subset of correct assignments from a parsimonious set of 3D NMR experiments of (15)N, (13)C labeled proteins. Chemical shifts of sequential residue pairs are predicted from static protein structures using PPM_One, which are then compared with the corresponding experimental shifts. Globally optimized weighted matching identifies the assignments that are robust with respect to small changes in NMR cross-peak positions...
May 2015: Journal of Magnetic Resonance
Elena Schmidt, Teppei Ikeya, Mitsuhiro Takeda, Frank Löhr, Lena Buchner, Yutaka Ito, Masatsune Kainosho, Peter Güntert
The automated chemical shift assignment algorithm FLYA has been extended for use with stereo-array isotope labeled (SAIL) proteins to determine the sequence-specific resonance assignments of large proteins. Here we present the assignment of the backbone and sidechain chemical shifts of the 21kDa thioldisulfide oxidoreductase DsbA from Escherichia coli that were determined with the SAIL-FLYA algorithm in conjunction with automated peak picking. No manual corrections of peak lists or assignments were applied...
December 2014: Journal of Magnetic Resonance
Quynh Doan, Paul Enarson, Niranjan Kissoon, Terry P Klassen, David W Johnson
BACKGROUND: Pediatric acute respiratory infections (ARIs) represent a significant burden on pediatric Emergency Departments (EDs) and families. Most of these illnesses are due to viruses. However, investigations (radiography, blood, and urine testing) to rule out bacterial infections and antibiotics are often ordered because of diagnostic uncertainties. This results in prolonged ED visits and unnecessary antibiotic use. The risk of concurrent bacterial infection has been reported to be negligible in children over three months of age with a confirmed viral infection...
September 15, 2014: Cochrane Database of Systematic Reviews
Ahmed Abbas, Xianrong Guo, Bing-Yi Jing, Xin Gao
Despite significant advances in automated nuclear magnetic resonance-based protein structure determination, the high numbers of false positives and false negatives among the peaks selected by fully automated methods remain a problem. These false positives and negatives impair the performance of resonance assignment methods. One of the main reasons for this problem is that the computational research community often considers peak picking and resonance assignment to be two separate problems, whereas spectroscopists use expert knowledge to pick peaks and assign their resonances at the same time...
June 2014: Journal of Biomolecular NMR
Peter Refsing Andersen, Michal Domanski, Maiken S Kristiansen, Helena Storvall, Evgenia Ntini, Celine Verheggen, Aleks Schein, Jakob Bunkenborg, Ina Poser, Marie Hallais, Rickard Sandberg, Anthony Hyman, John LaCava, Michael P Rout, Jens S Andersen, Edouard Bertrand, Torben Heick Jensen
Nuclear processing and quality control of eukaryotic RNA is mediated by the RNA exosome, which is regulated by accessory factors. However, the mechanism of exosome recruitment to its ribonucleoprotein (RNP) targets remains poorly understood. Here we report a physical link between the human exosome and the cap-binding complex (CBC). The CBC associates with the ARS2 protein to form CBC-ARS2 (CBCA) and then further connects, together with the ZC3H18 protein, to the nuclear exosome targeting (NEXT) complex, thus forming CBC-NEXT (CBCN)...
December 2013: Nature Structural & Molecular Biology
Bin-Bin Li, Chuan-Xiang Zhou, Sheng-Nan Jia
Cribriform type of salivary basal cell adenoma (cBCA) is relatively rare and problematic in distinction from adenoid cystic carcinoma (AdCC). The aim of this study was to investigate the clinicopathology and immunoprofile of cBCA. Nineteen cases of cBCA with at least a 30% area of cribriform structure under microscope were analyzed by the description of their histopathologic and immunohistochemical features using the antibodies of matrix metalloproteinase-9 (MMP9), CK8&18, calponin, SMA, S100, P63, CD117, and laminin...
February 2014: Annals of Diagnostic Pathology
Kaustubh Sinha, Linda Jen-Jacobson, Gordon S Rule
The HMCM [CG]CBCA experiment (Tugarinov and Kay in J Am Chem Soc 125:13868-13878, 2003) correlates methyl carbon and proton shifts to Cγ, Cβ, and Cα resonances for the purpose of resonance assignments. The relative sensitivity of the HMCM[CG]CBCA sequence experiment is compared to a divide-and-conquer approach to assess whether it is best to collect all of the methyl correlations at once, or to perform separate experiments for each correlation. A straightforward analysis shows that the divide-and-conquer approach is intrinsically more sensitive, and should always be used to obtain methyl-Cγ, Cβ, and Cα correlations...
August 2013: Journal of Biomolecular NMR
Catherine M Pirkle, Alexandre Dumont, Mamadou Traoré, Maria-Victoria Zunzunegui
BACKGROUND: Maternal mortality in referral hospitals in Mali and Senegal surpasses 1% of obstetrical admissions. Poor quality obstetrical care contributes to high maternal mortality; however, poor care is often linked to insufficient hospital resources. One promising method to improve obstetrical care is maternal death review. With a cluster randomized trial, we assessed whether an intervention, based on maternal death review, could improve obstetrical quality of care. METHODS: The trial began with a pre-intervention year (2007), followed by two years of intervention activities and a post-intervention year...
2013: BMC Pregnancy and Childbirth
Nizar Happyana, Sara Agnolet, Remco Muntendam, Annie Van Dam, Bernd Schneider, Oliver Kayser
Trichomes, especially the capitate-stalked glandular hairs, are well known as the main sites of cannabinoid and essential oil production of Cannabis sativa. In this study the distribution and density of various types of Cannabis sativa L. trichomes, have been investigated by scanning electron microscopy (SEM). Furthermore, glandular trichomes were isolated over the flowering period (8 weeks) by laser microdissection (LMD) and the cannabinoid profile analyzed by LCMS. Cannabinoids were detected in extracts of 25-143 collected cells of capitate-sessile and capitate stalked trichomes and separately in the gland (head) and the stem of the latter...
March 2013: Phytochemistry
Catherine M Pirkle, Alexandre Dumont, Mamadou Traore, Maria-Victoria Zunzunegui
BACKGROUND: In Mali and Senegal, over 1% of women die giving birth in hospital. At some hospitals, over a third of infants are stillborn. Many deaths are due to substandard medical practices. Criterion-based clinical audits (CBCA) are increasingly used to measure and improve obstetrical care in resource-limited settings, but their measurement properties have not been formally evaluated. In 2011, we published a systematic review of obstetrical CBCA highlighting insufficient considerations of validity and reliability...
2012: BMC Pregnancy and Childbirth
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"