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https://www.readbyqxmd.com/read/28920853/flavobacterium-chuncheonense-sp-nov-and-flavobacterium-luteum-sp-nov-isolated-from-a-freshwater-lake
#1
Miri Park, Gi Gyun Nam, Suhyun Kim, Hyoung Tae Jeon, Yochan Joung, Jang-Cheon Cho
Two Gram-staining-negative, orange coloured, non-motile, rod-shaped bacterial strains, designated strains IMCC26013T and IMCC26026T, were isolated from a freshwater sample collected from Lake Soyang in Korea. The 16S rRNA gene-based phylogenetic analyses showed that both strains belonged to the genus Flavobacterium and that strains IMCC26013T and IMCC26026T were most closely related to Flavobacterium psychrophilum (96.5 %) and Flavobacterium myungsuense (97.7 %), respectively. DNA G+C contents of strains IMCC26013T and IMCC26026T were 37...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920848/dyella-agri-sp-nov-isolated-from-reclaimed-grassland-soil
#2
Dhiraj Kumar Chaudhary, Jaisoo Kim
A novel strain, DKC-1T, was isolated from reclaimed grassland soil and was characterized taxonomically by a polyphasic approach. Strain DKC-1T was a Gram-staining-negative, light-yellow-coloured and rod shaped bacterium, motile with polar flagellum. It was able to grow at 20-37 °C, at pH 4.5-9.0 and with 0-3 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain DKC-1T formed a clade within the members of the genus Dyella and showed highest sequence similarities to Dyella japonica XD53T (98...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920840/flavobacterium-dispersum-sp-nov-isolated-from-a-freshwater-spring
#3
Wen-Ming Chen, Ching-Ling Su, Shih-Yi Sheu
A novel bacterial strain MVW-23T was isolated from a freshwater spring in Taiwan. The strain was Gram-staining-negative, strictly aerobic, motile by gliding, rod-shaped and formed translucent yellow colonies. Optimal growth occurred at 20-30 °C, pH 7.0, and in the presence of 0.5-1 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MVW-23T belonged to the genus Flavobacterium and showed the highest levels of sequence similarity with respect to Flavobacterium denitrificans ED5T (97...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920838/edaphobaculum-flavum-gen-nov-sp-nov-a-member-of-family-chitinophagaceae-isolated-from-grassland-soil
#4
Min Cao, Jing Huang, Jingxin Li, Zixu Qiao, Gejiao Wang
A yellow-coloured, Gram-stain-negative, non-motile, rod-shaped, strictly aerobic bacterium, designated 1-116T, was isolated from Erdos grassland soil of Inner Mongolia, PR China. Phylogenetic analysis based on 16S rRNA genes showed that strain 1-116T was a member of family Chitinophagaceae and exhibited the highest similarities to Taibaiella koreensis THG-DT86T (90.1 %) and Flavihumibacter solisivae 3-3T (90.0 %), while the similarities to the other Chitinophagaceae type strains were lower than 90.0 %...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920829/allobranchiibius-huperziae-gen-nov-sp-nov-a-member-of-dermacoccaceae-isolated-from-the-root-of-a-medicinal-plant-huperzia-serrata-thunb
#5
Meng-Jie Ai, Ye Sun, Hong-Min Sun, Hong-Yu Liu, Li-Yan Yu, Yu-Qin Zhang
A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077T, was isolated from the surface-sterilized root of a medicinal plant Huperzia serrata (Thunb.) collected from Sichuan Province, south-west China. The peptidoglycan type of strain CPCC 204077T was detected as A4α with an l-Lys-l-Ser-d-Asp interpeptide bridge. Galactose, glucose, rhamnose and ribose were the sugar compositions in the whole-cell hydrolysates. MK-8(H4) was the only menaquinone. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, one unidentified phospholipid and one unidentified glycolipid...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920828/paenibacillus-arcticus-sp-nov-isolated-from-arctic-soil
#6
In-Tae Cha, Eui-Sang Cho, Yesol Yoo, Yoon Ji Seok, Inhye Park, Hee Seon Lim, Jung-Min Park, Seong Woon Roh, Young-Do Nam, Hak-Jong Choi, Yoo Kyung Lee, Myung-Ji Seo
A Gram-positive, endospore-forming, strictly aerobic and rod-shaped bacterium, designated strain MME2_R6T, was isolated from Arctic soil, and it was identified by using a polyphasic taxonomic approach. This strain was psychrotolerant, growing at 4‒24 °C. 16S rRNA gene sequence analysis showed that strain MME2_R6T was closest to Paenibacillus swuensis DY6T, with 93.9 % similarity. However, in phylogenetic analysis based on the 16S rRNA gene sequence, strain MME2_R6T showed that it clustered with Paenibacillus contaminans CKOBP-6T and the sequencing similarity between the two species was 93...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28919838/adherence-to-precautions-for-preventing-the-transmission-of-microorganisms-in-primary-health-care-a-qualitative-study
#7
Michely Aparecida Cardoso Maroldi, Adriana Maria da Silva Felix, Ana Angélica Lima Dias, Julia Yaeko Kawagoe, Maria Clara Padoveze, Sílvia Alice Ferreira, Sílvia Helena Zem-Mascarenhas, Stephen Timmons, Rosely Moralez Figueiredo
BACKGROUND: Health care associated infections (HAIs) are a source of concern worldwide. No health service in any country can be considered HAI risk-free. However, there is scarcity of data on the risks to which both patients and health workers are subject in non-hospital settings. The aim of this study was to identify issues that determine the adherence of professionals to precautions for preventing transmission of microorganisms in primary health care. METHOD: This was a qualitative study, using focus groups of primary health care staff, in two Brazilian municipalities...
2017: BMC Nursing
https://www.readbyqxmd.com/read/28919459/genome-content-metabolic-pathways-and-biotechnological-potential-of-the-psychrophilic-arctic-bacterium-psychrobacter-sp-dab_al43b-a-source-and-a-host-of-novel-psychrobacter-specific-vectors
#8
Robert Lasek, Lukasz Dziewit, Anna Ciok, Przemyslaw Decewicz, Krzysztof Romaniuk, Zuzanna Jedrys, Daniel Wibberg, Andreas Schlüter, Alfred Pühler, Dariusz Bartosik
Psychrobacter sp. DAB_AL43B, isolated from ornithogenic soil collected on the Arctic island of Spitsbergen, is a newly sequenced psychrophilic strain susceptible to conjugation and electrotransformation. Its genome consists of a circular chromosome (3.3 Mb) and four plasmids (4.4-6.4kb). In silico genome mining and microarray-based phenotypic analysis were performed to describe the metabolic potential of this strain and identify possible biotechnological applications. Metabolic reconstruction indicated that DAB_AL43B prefers low-molecular-weight carboxylates and amino acids as carbon and energy sources...
September 14, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28918630/assessing-the-reliability-of-mfa-results-the-cases-of-rhenium-gallium-and-germanium-in-the-us-economy
#9
Grégoire Meylan, Barbara K Reck, Helmut Rechberger, Thomas E Graedel, Oliver Schwab
Decision-makers traditionally expect "hard facts" from scientific inquiry, an expectation the results of Material Flow Analyses (MFAs) can hardly meet. MFA limitations are attributable to incompleteness of flowcharts, limited data quality, and model assumptions. Moreover, MFA results are mostly less based on empirical observation and rather on social knowledge construction processes. Developing, applying, and improving means for evaluating and communicating reliability of MFA results is imperative. We apply two recently proposed approaches for making quantitative statements on MFA reliability to national minor metals systems: rhenium, gallium, and germanium in the US in 2012...
September 18, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28918434/improved-dna-purification-with-quality-assurance-for-evaluation-of-the-microbial-genetic-content-of-constructed-wetlands
#10
Wenda Huang, Jingjing Guo, Ran Tao, Ying Man, Yunv Dai, Yang Yang
Efficient isolation of target DNA is a crucial first step of DNA-based metagenomic analyses of environmental samples. Insufficient quantity and purity of DNA isolated using commercial kits result in missing genetic information, especially for large-diameter substrates in constructed wetlands (CWs). Here, we addressed this problem by devising a cost-effective calcium chloride lysozyme-sodium dodecyl sulfate (SDS) method (CCLS), with key improvements in the steps of humic acid removal and cell lysis. The buffer comprising Tris, EDTA, Na2O2P7 and PVPP (TENP), and skim milk, could reduce adsorption between microorganisms and substrates, and calcium chloride precipitated and removed over 94% of humic acid...
September 16, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28916908/capsular-contracture-rate-after-breast-augmentation-with-periareolar-versus-other-two-inframammary-and-transaxillary-incisions-a-meta-analysis
#11
REVIEW
Shangshan Li, Lin Chen, Wenyue Liu, Dali Mu, Jie Luan
BACKGROUND: Capsular contracture has been the most common complication of cosmetic breast augmentation. The effect of incision pattern on capsular contracture is still unclear. This meta-analysis demonstrates current evidence with regard to the comparison of capsular contracture rate between periareolar and other two (transaxillary and inframammary) incisions. METHODS: PubMed, EMBASE, and Cochrane databases were searched up to January 2017. The results of selected studies were meta-analyzed to obtain a pooled odds ratio of the effect of periareolar versus other two incision patterns (transaxillary or inframammary incision) of breast augmentation on capsular contracture rates...
September 15, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28916228/cellular-therapy-injections-in-today-s-orthopedic-market-a-social-media-analysis
#12
Prem N Ramkumar, Sergio M Navarro, Heather S Haeberle, Morad Chughtai, Christopher Demetriades, Nicolas S Piuzzi, Michael A Mont, Thomas W Bauer, George F Muschler
BACKGROUND: The current state of cellular therapy for musculoskeletal conditions is at a crossroads. Marketing efforts are often outpacing clinical evidence and regulatory control. QUESTIONS/PURPOSES: This study was an effort to describe the marketing of cellular therapy in musculoskeletal medicine by evaluating the content in popular social media channels. Specifically, media posts were evaluated for the following: (1) perspective, (2) tone, (3) content and (4) visibility...
September 12, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28915890/barriers-and-enablers-for-the-development-and-implementation-of-allied-health-clinical-practice-guidelines-in-south-african-primary-healthcare-settings-a-qualitative-study
#13
J M Dizon, K Grimmer, Q Louw, S Machingaidze, H Parker, H Pillen
BACKGROUND: The South African allied health (AH) primary healthcare (PHC) workforce is challenged with the complex rehabilitation needs of escalating patient numbers. The application of evidence-based care using clinical practice guidelines (CPGs) is one way to make efficient and effective use of resources. Although CPGs are common for AH in high-income countries, there is limited understanding of how to do this in low- to middle-income countries. This paper describes barriers and enablers for AH CPG uptake in South African PHC...
September 15, 2017: Health Research Policy and Systems
https://www.readbyqxmd.com/read/28914971/extending-data-worth-analyses-to-select-multiple-observations-targeting-multiple-forecasts
#14
Troels N Vilhelmsen, Ty P A Ferré
Hydrological models are often set up to provide specific forecasts of interest. Owing to the inherent uncertainty in data used to derive model structure and used to constrain parameter variations, the model forecasts will be uncertain. Additional data collection is often performed to minimize this forecast uncertainty. Given our common financial restrictions, it is critical that we identify data with maximal information content with respect to forecast of interest. In practice, this often devolves to qualitative decisions based on expert opinion...
September 15, 2017: Ground Water
https://www.readbyqxmd.com/read/28914501/andrology-of-male-to-female-transsexuals-influence-of-cross-sex-hormone-therapy-on-testicular-function
#15
REVIEW
F Schneider, S Kliesch, S Schlatt, N Neuhaus
Patients with gender dysphoria are offered cross-sex hormone therapy and sex reassignment surgery to achieve the transition between the sex assigned at birth and gender identity. According to international guidelines, cross-sex hormone therapy in trans-women should lead to a psychologically and physiologically healthy body with feminized serum hormone levels, resulting in suppression of spermatogenesis. However, in a recently published multi-center study, we discovered a high proportion of patients with male serum hormone levels and qualitatively intact spermatogenesis on the day of sex reassignment surgery...
September 2017: Andrology
https://www.readbyqxmd.com/read/28913828/diet-and-body-shape-changes-of-p%C3%A4-roko-kelloggella-disalvoi-gobiidae-from-intertidal-pools-of-easter-island
#16
J Vera-Duarte, C A Bustos, M F Landaeta
This study assesses seasonal variation in the morphology and diet of juveniles and adults of the Easter Island endemic goby Kelloggella disalvoi from intertidal pools during September-October 2015 (spring) and June-July 2016 (winter), utilizing geometric morphometric and gut-content analyses. A set of 16 landmarks was digitized in 128 individuals. Shape changes related to size changes (i.e. allometry) were low (18·6%) and were seasonally similar. Body shape changes were mainly dorsoventral (44·2% of variance) and comprised posteroventral displacement of the premaxilla and bending of the body...
September 15, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28912544/development-of-chloroplast-genome-resources-for-peanut-arachis-hypogaea-l-and-other-species-of-arachis
#17
Dongmei Yin, Yun Wang, Xingguo Zhang, Xingli Ma, Xiaoyan He, Jianhang Zhang
Peanut (Arachis hypogaea L.) is an important oilseed and cash crop worldwide. Wild Arachis spp. are potental sources of novel genes for the genetic improvement of cultivated peanut. Understanding the genetic relationships with cultivated peanut is important for the efficient use of wild species in breeding programmes. However, for this genus, only a few genetic resources have been explored so far. In this study, new chloroplast genomic resources have been developed for the genus Arachis based on whole chloroplast genomes from seven species that were sequenced using next-generation sequencing technologies...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28911881/tailoring-the-orthopaedic-consultation-how-perceived-patient-characteristics-influence-surgeons-communication
#18
Tessa Dekkers, Marijke Melles, Nina M C Mathijssen, Stephan B W Vehmeijer, Huib de Ridder
OBJECTIVE: To investigate whether and how orthopaedic surgeons tailor communication during medical consultations based on perceived patient characteristics. METHODS: Seven orthopaedic surgeons were repeatedly interviewed following an approach based on ecological momentary assessment. Qualitative content analysis was used to analyse the eighty short interviews. The association between patient characteristics and tailoring approaches was explored in a correspondence analysis of the counted codes...
September 1, 2017: Patient Education and Counseling
https://www.readbyqxmd.com/read/28911272/exploring-health-navigating-design-momentary-contentment-in-a-cancer-context
#19
Ulrika Sandén, Lars Harrysson, Hans Thulesius, Fredrik Nilsson
PURPOSE: The technocratic and medicalized model of healthcare is rarely optimal for patients. By connecting two different studies we explore the possibilities of increasing quality of life in cancer care. METHODS: The first study captures survival strategies in a historically isolated Arctic village in Norway resulting in Momentary contentment theory, which emerged from analysing four years of participant observation and interview data. The second study conceptualizes everyday life of cancer patients based on in-depth interviews with 19 cancer patients; this was conceptualized as Navigating a new life situation...
2017: International Journal of Qualitative Studies on Health and Well-being
https://www.readbyqxmd.com/read/28906545/adipose-tissue-content-of-saturated-fatty-acids-and-atrial-fibrillation-a-case-cohort-study
#20
Pia Thisted Dinesen, Thomas Andersen Rix, Albert Marni Joensen, Anne Tjønneland, Søren Lundbye-Christensen, Kim Overvad, Erik Berg Schmidt
OBJECTIVES: The aim of the present study was to investigate the association between adipose tissue content of total saturated fatty acids including myristic (C14:0), palmitic (C16:0) and stearic (C18:0) acid, as a measure of exposure to saturated fatty acids and the risk of incident atrial fibrillation. MATERIALS AND METHODS: A total of 57,053 Danish men and women aged 50-64 years participating in the Diet, Cancer and Health cohort had an adipose tissue biopsy taken at baseline and this was analysed for saturated fatty acids content by gas chromatography...
September 14, 2017: European Journal of Clinical Investigation
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