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https://www.readbyqxmd.com/read/28820118/arcobacter-haliotis-sp-nov-isolated-from-abalone-species-haliotis-gigantea
#1
Reiji Tanaka, Ilse Cleenwerck, Yukino Mizutani, Shunpei Iehata, Peter Bossier, Peter Vandamme
A Gram-negative, aerobic, polar-flagellated and rod-shaped, sometimes slightly curved bacterium, designated MA5T, was isolated from the gut of an abalone of the species Haliotis gigantea collected in Japan. Phylogenetic analyses based on 16S rRNA, gyrB, hsp60 and rpoB gene sequences placed strain MA5T in the genus Arcobacter in an independent phylogenetic line. Comparison of the 16S rRNA gene sequence of this strain with those of the type strains of the established Arcobacter species revealed A. nitrofigilis (95...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820117/mucilaginibacter-rubeus-sp-nov-isolated-from-rhizosphere-soil
#2
Jie-Chao Wei, Le-Ni Sun, Zhuo-Xin Yuan, Xue-Ting Hou, En-Dong Yang, Yuan-Yuan Cao
A Gram-stain-negative, aerobic, non-motile, rod-shaped and non-spore-forming bacterium, designated EF23T, was isolated from rhizosphere soil of watermelon. Growth of strain EF23T was observed at 10-37 °C, at pH 5.0-9.0 and in the presence of 0-0.5 % (w/v) NaCl. Strain EF23T contained menaquinone 7 (MK-7) as the major isoprenoid quinone, and summed feature 3 (C16:1ω7c and/or iso-C15 : 0 2-OH), iso-C15 : 0, C16 : 0 and iso-C17 : 0 3-OH as the major fatty acids. Phosphatidylethanolamine was identified as the major polar lipid...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820116/sphingobium-naphthae-sp-nov-with-the-ability-to-degrade-aliphatic-hydrocarbons-isolated-from-oil-contaminated-soil
#3
Dhiraj Kumar Chaudhary, Seung-Woo Jeong, Jaisoo Kim
A light yellow-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, designated strain K-3-6T, capable of degrading aliphatic hydrocarbons was isolated from oil-contaminated soil of Biratnagar, Morang, Nepal. It was able to grow at 15-45 °C, at pH 5.0-9.5 and with 0-6 % (w/v) NaCl. Based on 16S rRNA gene sequence analysis, strain K-3-6T belongs to the genus Sphingobium and is closely related to Sphingobium olei IMMIB HF-1T (98.4 % similarity), Sphingobium abikonense NBRC 16140T (98.3 %), Sphingobium rhizovicinum CC-FH12-1T (97...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820099/hymenobacter-pallidus-sp-nov-isolated-from-a-freshwater-fish-culture-pond
#4
Shih-Yi Sheu, Yi-Shu Li, Chiu-Chung Young, Wen-Ming Chen
A bacterial strain designated LYH-12T was isolated from a freshwater fish culture pond in Taiwan, ROC and characterized by taking a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain LYH-12T belonged to the genus Hymenobacter and was most closely related to Hymenobacter xinjiangensis X2-1gT and Hymenobacter rigui WPCB131T with a sequence similarity of 96.6 % and less than 96.5 % with other members of the genus. Cells of strain LYH-12T were Gram-stain-negative, aerobic, non-motile rods that were covered by large capsules and formed light pink-coloured colonies...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820096/lactobacillus-caviae-sp-nov-an-obligately-heterofermentative-bacterium-isolated-from-the-oral-cavity-of-a-guinea-pig-cavia-aperea-f-porcellus
#5
Jiri Killer, Radko Pechar, Pavel Švec, Hana Salmonová, Roman Švejstil, Martina Geigerová, Vojtěch Rada, Eva Vlková, Chahrazed Mekadim
A Gram-stain-positive, facultatively anaerobic, and catalase- and oxidase-negative bacterial strain designated MOZM2T, having 98.4 % 16S rRNA gene sequence identity with Lactobacillus reuteri DSM 20016T, was isolated from a swab of the oral cavity of a home-bred guinea pig. Comparative analyses based on the hsp60, pheS and tuf genes confirmed L. reuteri as its closest relative species, with calculated sequence similarities of 92.8, 88.8 and 96.9 %, respectively. DNA-DNA hybridisation revealed a 42 % degree of genetic similarity between the novel strain and L...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820091/alloscardovia-venturai-sp-nov-a-fructose-6-phosphate-phosphoketolase-positive-species-isolated-from-the-oral-cavity-of-a-guinea-pig-cavia-aperea-f-porcellus
#6
Hana Sechovcová, Jiri Killer, Radko Pechar, Martina Geigerová, Roman Švejstil, Hana Salmonová, Chahrazed Mekadim, Vojtěch Rada, Eva Vlková, Olga Kofroňová, Oldřich Benada
A slightly irregular, short rod-shaped bacterial strain, MOZIV/2T, showing activity of fructose 6-phosphate phosphoketolase was isolated from the oral cavity of a home-bred guinea-pig. Based on comparative 16S rRNA gene sequence analyses, its closest relatives were Alloscardovia omnicolens DSM 21503T and Alloscardovia criceti DSM 17774T with 96.0 and 95.6 % pairwise similarities, respectively. Completeness of the compared sequences was 97.3 and 96.9 %, respectively. Growth was found only under anaerobic conditions...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28820088/bacillus-praedii-sp-nov-isolated-from-purplish-paddy-soil
#7
Bo Liu, Guo-Hong Liu, Cetin Sengonca, Peter Schumann, Jie-Ping Wang, Yu-Jing Zhu, Qin-Ying Liu, Ming-Kuang Wang
A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium, designated strain FJAT-25547T, was isolated from the purplish paddy soil collected from Linshan Township, Yanting Prefecture of Sichuan Province in PR China (31° 16' N 105° 27' E). Growth was achieved aerobically at temperatures between 15 and 40 °C (optimum 30 °C), with between 0 and 10.0 % NaCl (w/v) (optimum 4 %) and in the range of pH 5.0-12.0 (optimum pH 9.0). The cell-wall peptidoglycan contained meso-diaminopimelic acid, and the main isoprenoid quinone was MK-7...
August 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28818650/recombinant-vs-native-anisakis-haemoglobin-ani-s-13-its-appraisal-as-a-new-gold-standard-for-the-diagnosis-of-allergy
#8
Juan González-Fernández, Luis Rivas, Juan Román Luque-Ortega, Rafael Núñez-Ramírez, Pamela Campioli, Teresa Gárate, María J Perteguer, Alvaro Daschner, Carmen Cuéllar
Recombinant allergens are currently the best option for serodiagnosis of human anisakiasis in terms of sensitivity and specificity. However, previous reports showed high rates of anisakiasis patients who were negative to Ani s 7 and especially to Ani s 1. Recently, Anisakis haemoglobin was described as a major allergen (Ani s 13). Although Ani s 13 belongs to a conserved protein family, it seems not to be a cross-reacting antigen because of the absence of IgE recognition against Ascaris haemoglobin in Anisakis patients...
August 14, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28817082/omega-3-long-chain-fatty-acids-in-the-heart-kidney-liver-and-plasma-metabolite-profiles-of-australian-prime-lambs-supplemented-with-pelleted-canola-and-flaxseed-oils
#9
Don V Nguyen, Van H Le, Quang V Nguyen, Bunmi S Malau-Aduli, Peter D Nichols, Aduli E O Malau-Aduli
The objective of the study was to ascertain whether human health beneficial omega-3 long-chain (≥C20) polyunsaturated fatty acid (n-3 LC-PUFA) content in heart, kidney and liver can be enhanced by supplementing prime lambs with graded levels of canola and flaxseed oil. Health status of the lambs, as a consequence of the supplementation, was also investigated by examining their plasma metabolites. Sixty purebred and first-cross lambs were allocated to one of five treatments of lucerne hay basal diet supplemented with isocaloric and isonitrogenous wheat-based pellets without oil inclusion (Control) or graded levels of canola oil at 2...
August 17, 2017: Nutrients
https://www.readbyqxmd.com/read/28814974/time-resolved-transcriptome-analysis-and-lipid-pathway-reconstruction-of-the-oleaginous-green-microalga-monoraphidium-neglectum-reveal-a-model-for-triacylglycerol-and-lipid-hyperaccumulation
#10
Daniel Jaeger, Anika Winkler, Jan H Mussgnug, Jörn Kalinowski, Alexander Goesmann, Olaf Kruse
BACKGROUND: Oleaginous microalgae are promising production hosts for the sustainable generation of lipid-based bioproducts and as bioenergy carriers such as biodiesel. Transcriptomics of the lipid accumulation phase, triggered efficiently by nitrogen starvation, is a valuable approach for the identification of gene targets for metabolic engineering. RESULTS: An explorative analysis of the detailed transcriptional response to different stages of nitrogen availability was performed in the oleaginous green alga Monoraphidium neglectum...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28814265/a-genome-wide-transcriptome-map-of-pistachio-pistacia-vera-l-provides-novel-insights-into-salinity-related-genes-and-marker-discovery
#11
Maryam Moazzzam Jazi, Seyed Mahdi Seyedi, Esmaeil Ebrahimie, Mansour Ebrahimi, Gianluca De Moro, Christopher Botanga
BACKGROUND: Pistachio (Pistacia vera L.) is one of the most important commercial nut crops worldwide. It is a salt-tolerant and long-lived tree, with the largest cultivation area in Iran. Climate change and subsequent increased soil salt content have adversely affected the pistachio yield in recent years. However, the lack of genomic/global transcriptomic sequences on P. vera impedes comprehensive researches at the molecular level. Hence, whole transcriptome sequencing is required to gain insight into functional genes and pathways in response to salt stress...
August 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28813175/qualitative-analysis-of-registered-nurses-perceptions-of-lactation-assessment-tools-why-and-how-they-are-completed
#12
Joan Esper Kuhnly, Donna J Chapman
BACKGROUND: Although lactation assessment tools are consistently used in clinical practice, there is no evidence describing registered nurses' perspectives regarding the purpose and thought processes involved when conducting a breastfeeding assessment. Research aim: This study aimed to explore registered nurses' perceptions on the purpose of lactation assessment tools and the thought processes involved in completing one. METHODS: Seven focus groups were held from April 2015 through July 2015, in coordination with regional and international lactation and perinatal conferences...
August 1, 2017: Journal of Human Lactation: Official Journal of International Lactation Consultant Association
https://www.readbyqxmd.com/read/28812844/clinician-perspectives-of-barriers-to-effective-implementation-of-a-rapid-response-system-in-an-academic-health-centre-a-focus-group-study
#13
John Rihari-Thomas, Michelle DiGiacomo, Jane Phillips, Phillip Newton, Patricia M Davidson
BACKGROUND: Systemic and structural issues of rapid response system (RRS) models can hinder implementation. This study sought to understand the ways in which acute care clinicians (physicians and nurses) experience and negotiate care for deteriorating patients within the RRS. METHODS: Physicians and nurses working within an Australian academic health centre within a jurisdictional-based model of clinical governance participated in focus group interviews. Verbatim transcripts were analysed using thematic content analysis...
January 1, 2017: International Journal of Health Policy and Management
https://www.readbyqxmd.com/read/28811619/comparative-mitogenomic-analysis-of-aposthonia-borneensis-and-aposthonia-japonica-embioptera-oligotomidae-reveals-divergent-evolution-of-webspinners
#14
Zhi-Teng Chen, Liang Lü, Ming-Xing Lu, Yu-Zhou Du
In this study, we report the complete mitochondrial genome (mitogenome, mtDNA) of Aposthonia borneensis and compare it with another sequenced webspinner, Aposthonia japonica. The A. borneensis mitogenome is smaller than A. japonica, but the size of each gene and the A + T content of protein-coding genes (PCGs) are almost identical in the two mitogenomes. Among the PCGs, atp6 shows the highest evolutionary rate and cox1 the lowest. The mtDNA map in A. borneensis is similar to Drosophila yakuba, but distinctly different from A...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28809147/flavobacterium-flaviflagrans-sp-nov-a-bacterium-of-the-family-flavobacteriaceae-isolated-from-forest-soil
#15
Ram Hari Dahal, Dhiraj Kumar Chaudhary, Jaisoo Kim
Two strains of bacteria designated CB-3T and CB-31 were isolated from Kyonggi University forest soil. Cells were aerobic, Gram-stain-negative, oxidase-positive, non-motile, non-spore-forming, rod-shaped and yellow-pigmented. They were able to grow at 15-42 °C, pH 5.5-9.5 and with 0-1.5 % (w/v) NaCl concentration. Flexirubin-type pigments were absent. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains CB-3T and CB-31 formed a lineage within the family Flavobacteriaceae of the phylum Bacteroidetes that was distinct from various species of the genus Flavobacterium, including Flavobacterium humicola UCM-46T (99...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28809144/defluviimonas-nitratireducens-sp-nov-isolated-from-surface-seawater
#16
Yang Liu, Qiliang Lai, Wanpeng Wang, Zongze Shao
A bacterial strain, designated DL5-4T, was isolated from the surface seawater of Dalian Bay and characterized using a polyphasic taxonomy approach. Cells of DL5-4T were Gram-staining-negative, non-motile and short-rod-shaped. Growth was observed at 8-40 °C (optimum 28-30 °C), at pH 6-9 (optimum pH 7) and in 0-7 % NaCl (optimum 1-3 %, w/v). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that DL5-4T formed an independent branch with members of the genus Defluviimonas, sharing high similarities with five related type strains, Defluviimonas aquaemixtae CDM-7T (96...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28809143/bacillus-swezeyi-sp-nov-and-bacillus-haynesii-sp-nov-isolated-from-desert-soil
#17
Christopher A Dunlap, David A Schisler, Elizabeth B Perry, Nora Connor, Frederick M Cohan, Alejandro P Rooney
Two isolates of Gram-reaction-positive, facultatively anaerobic, motile, rod-shaped, endospore-forming bacteria were identified during a survey of the diversity of strains belonging to the genus Bacillus deposited in the Agriculture Research Service Culture Collection. These strains were originally isolated from soil in Evolution Canyon III (Israel) in a survey of ecological diversification. Phylogenetic analysis of the 16S rRNA gene of strains NRRL B-41294T and NRRL B-41327T determined they were closely related to members of the Bacillus licheniformis clade...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28809142/massilia-agri-sp-nov-isolated-from-reclaimed-grassland-soil
#18
Dhiraj Kumar Chaudhary, Jaisoo Kim
A light yellow-coloured, Gram-stain-negative, motile and rod-shaped bacterium, designated strain K-3-1T, was isolated from reclaimed grassland soils of Belbari, Morang, Nepal. It was able to grow at 4-45 °C, at pH 5.0-10.0, and at 0-2 % (w/v) NaCl concentrations. This strain was taxonomically characterized by a polyphasic approach. Based on the 16S rRNA gene sequence analysis, strain K-3-1T belongs to the genus Massilia and is closely related to Massilia consociata CCUG 58010T (98.3 % sequence similarity), Massilia tieshanensis TS3T (98...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28809137/paracoccus-mangrovi-sp-nov-isolated-from-a-mangrove
#19
Wen-Ming Chen, Yi-Shu Li, Chiu-Chung Young, Shih-Yi Sheu
A bacterial strain, designated gyp-1T, was isolated from a mangrove in Taiwan and characterized using the polyphasic taxonomic approach. Cells of gyp-1T were Gram-staining-negative, aerobic, poly-β-hydroxybutyrate-accumulating, non-motile, coccoid or short-rod-shaped and formed cream-coloured colonies. Growth occurred at 15-37 °C (optimum, 25-30 °C), at pH 5.5-7.0 (optimum, pH 6.0) and with 0-4 % NaCl (optimum, 1-2 %). Phylogenetic analyses based on 16S rRNA gene sequences indicated that gyp-1T represented a member of the genus Paracoccus and showed the highest levels of sequence similarity with respect to Paracoccus lutimaris HDM-25T (97...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28808326/the-importance-of-the-nutritive-value-of-old-bones-in-the-diet-of-bearded-vultures-gypaetus-barbatus
#20
Antoni Margalida, Daniel Villalba
Vultures are central-place foragers and need to optimize their foraging behaviour to offset travel costs by increasing their energy gain. This process is more obvious in certain vulture species that do not feed their young by regurgitation and so must carry food items back to the nest. The Bearded Vulture Gypaetus barbatus is the only species with a bone-diet based. We analysed the chemical composition of bones and the age-related changes in their nutritive value to assess the differences in energy content between bones of differing age, body part and species...
August 14, 2017: Scientific Reports
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