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https://www.readbyqxmd.com/read/28109190/qtl-mapping-and-candidate-genes-for-resistance-to-fusarium-ear-rot-and-fumonisin-contamination-in-maize
#1
Valentina Maschietto, Cinzia Colombi, Raul Pirona, Giorgio Pea, Francesco Strozzi, Adriano Marocco, Laura Rossini, Alessandra Lanubile
BACKGROUND: Fusarium verticillioides is a common maize pathogen causing ear rot (FER) and contamination of the grains with the fumonisin B1 (FB1) mycotoxin. Resistance to FER and FB1 contamination are quantitative traits, affected by environmental conditions, and completely resistant maize genotypes to the pathogen are so far unknown. In order to uncover genomic regions associated to reduced FER and FB1 contamination and identify molecular markers for assisted selection, an F2:3 population of 188 progenies was developed crossing CO441 (resistant) and CO354 (susceptible) genotypes...
January 21, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28108530/use-of-performance-metrics-for-the-measurement-of-universal-coverage-for-maternal-care-in-mexico
#2
Edson Serván-Mori, David Contreras-Loya, Octavio Gomez-Dantés, Gustavo Nigenda, Sandra G Sosa-Rubí, Rafael Lozano
This study provides evidence for those working in the maternal health metrics and health system performance fields, as well as those interested in achieving universal and effective health care coverage. Based on the perspective of continuity of health care and applying quasi-experimental methods to analyse the cross-sectional 2009 National Demographic Dynamics Survey (n = 14 414 women), we estimated the middle-term effects of Mexico's new public health insurance scheme, Seguro Popular de Salud (SPS) (vs women without health insurance) on seven indicators related to maternal health care (according to official guidelines): (a) access to skilled antenatal care (ANC); (b) timely ANC; (c) frequent ANC; (d) adequate content of ANC; (e) institutional delivery; (f) postnatal consultation and (g) access to standardized comprehensive antenatal and postnatal care (or the intersection of the seven process indicators)...
January 20, 2017: Health Policy and Planning
https://www.readbyqxmd.com/read/28107961/new-approach-of-a-transient-icp-ms-measurement-method-for-samples-with-high-salinity
#3
Christina Hein, Jonas Michael Sander, Ralf Kautenburger
In the near future it is necessary to establish a disposal for high level nuclear waste (HLW) in deep and stable geological formations. In Germany typical host rocks are salt or claystone. Suitable clay formations exist in the south and in the north of Germany. The geochemical conditions of these clay formations show a strong difference. In the northern ionic strengths of the pore water up to 5M are observed. The determination of parameters like Kd values during sorption experiments of metal ions like uranium or europium as homologues for trivalent actinides onto clay stones are very important for long term safety analysis...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107707/uremia-modulates-the-phenotype-of-aortic-smooth-muscle-cells
#4
Marie Madsen, Annemarie Aarup, Sebastian Albinsson, Karsten Hartvigsen, Charlotte M Sørensen, Karolina Turczynska, Lars B Nielsen, Tanja Xenia Pedersen
BACKGROUND AND AIMS: Chronic kidney disease leads to uremia and markedly accelerates atherosclerosis. Phenotypic modulation of smooth muscle cells (SMCs) in the arterial media plays a key role in accelerating atherogenesis. The aim of this study was to investigate whether uremia per se modulates the phenotype of aortic SMCs in vivo. METHODS: Moderate uremia was induced by 5/6 nephrectomy in apolipoprotein E knockout (ApoE(-/-)) and wildtype C57Bl/6 mice. Plasma analysis, gene expression, histology, and myography were used to determine uremia-mediated changes in the arterial wall...
December 23, 2016: Atherosclerosis
https://www.readbyqxmd.com/read/28105505/frankia-discariae-sp-nov-an-infective-and-effective-microsymbiont-isolated-from-the-root-nodule-of-discaria-trinervis
#5
Imen Nouioui, Maria Del Carmen Montero-Calasanz, Faten Ghodhbane-Gtari, Manfred Rohde, Louis S Tisa, Hans-Peter Klenk, Maher Gtari
Strain BCU110501(T) was the first isolate reported to fulfill Koch's postulates by inducing effective nodules on its host plant of origin Discaria trinervis (Rhalmnaceae). Based on 16S rRNA gene sequence similarities, the strain was found to be most closely related to the type strain of Frankia elaeagni DSM 46783(T) (98.6%) followed by F. alni DSM 45986(T) (98.2%), F. casuarinae DSM 45818(T) (97.8%) and F. inefficacies DSM 45817(T) (97.8%). Digital DNA:DNA hybridizations (dDDH) between strain BCU110501(T)and the type strains of other Frankia species were clearly below the cutoff point of 70%...
January 19, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28104596/operationalising-ethical-challenges-in-dementia-research-a-systematic-review-of-current-evidence
#6
Emily West, Astrid Stuckelberger, Sophie Pautex, Janneke Staaks, Marjolein Gysels
BACKGROUND: the worldwide number of dementia cases is increasing, and this is a trend that is expected to continue as a growing proportion of the population ages. However, conducting research with persons suffering from dementia can be fraught due to fears surrounding research risks in vulnerable populations. This can make seeking approval for studies difficult. As research directly involving persons with dementia is key for the development of evidence-based best practice, the development of a coherent ethical strategy to perform such research feasibly and effectively is of paramount importance...
January 18, 2017: Age and Ageing
https://www.readbyqxmd.com/read/28103120/compassionate-options-for-pediatric-ems-cope-addressing-communication-skills
#7
Aaron W Calhoun, Erica R H Sutton, Anita P Barbee, Beth McClure, Carrie Bohnert, Richard Forest, Peter Taillac, Mary E Fallat
INTRODUCTION: Each year, 16,000 children suffer cardiopulmonary arrest, and in one urban study, 2% of pediatric EMS calls were attributed to pediatric arrests. This indicates a need for enhanced educational options for prehospital providers that address how to communicate to families in these difficult situations. In response, our team developed a cellular phone digital application (app) designed to assist EMS providers in self-debriefing these events, thereby improving their communication skills...
January 19, 2017: Prehospital Emergency Care
https://www.readbyqxmd.com/read/28100309/nioella-sediminis-sp-nov-isolated-from-the-surface-sediment-and-emended-description-of-the-genus-nioella
#8
Yang Liu, Juan Du, Qiliang Lai, Chunming Dong, Zongze Shao
A bacterial strain, designated JS7-11T, was isolated from the surface sediment of the Jiulong River and characterized using a polyphasic taxonomic approach. Cells of strain JS7-11T was Gram-staining-negative, non-motile and short-rod-shaped. Growth was observed at 10-40°C (optimum 25-30°C), at pH 6-8 (optimum 7) and in 1-12% NaCl (optimum 3-4%, w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JS7-11T and type strain Nioella nitratireducens SSW136T formed an independent cluster, sharing 97...
January 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28098050/dairy-food-supplementation-may-reduce-malnutrition-risk-in-institutionalised-elderly
#9
Sandra Iuliano, Shirley Poon, Xiaofang Wang, Minh Bui, Ego Seeman
Malnutrition in institutionalised elderly increases morbidity and care costs. Meat and dairy foods are high-quality protein sources so adequate intakes may reduce malnutrition risk. We aimed to determine whether inadequate intakes of meat and dairy foods contribute to malnutrition in institutionalised elderly. This cross-sectional study involved 215 elderly residents (70·2 % females, mean age 85·8 years) from twenty-one aged-care facilities in Melbourne, Australia. Dietary intake was assessed using observed plate waste...
January 18, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28097059/the-complete-chloroplast-genome-sequence-of-an-endemic-monotypic-genus-hagenia-rosaceae-structural-comparative-analysis-gene-content-and-microsatellite-detection
#10
Andrew W Gichira, Zhizhong Li, Josphat K Saina, Zhicheng Long, Guangwan Hu, Robert W Gituru, Qingfeng Wang, Jinming Chen
Hagenia is an endangered monotypic genus endemic to the topical mountains of Africa. The only species, Hagenia abyssinica (Bruce) J.F. Gmel, is an important medicinal plant producing bioactive compounds that have been traditionally used by African communities as a remedy for gastrointestinal ailments in both humans and animals. Complete chloroplast genomes have been applied in resolving phylogenetic relationships within plant families. We employed high-throughput sequencing technologies to determine the complete chloroplast genome sequence of H...
2017: PeerJ
https://www.readbyqxmd.com/read/28096741/a-day-in-the-life-of-third-year-medical-students-using-an-ethnographic-method-to-understand-information-seeking-and-use
#11
Andrea B Twiss-Brooks, Ricardo Andrade, Michelle B Bass, Barbara Kern, Jonna Peterson, Debra A Werner
OBJECTIVE: The authors undertook this project to learn how third-year medical students seek and use information in the course of daily activities, especially activities conducted in clinical settings in a variety of institutions. METHODS: We recruited sixty-eight third-year undergraduate medical school students to create a mapping diary of a day that included clinical activities. We conducted semi-structured interviews based on the mapping diaries. Using content and thematic analyses of the resulting interview transcripts, we developed an ethnographic case study for each participant...
January 2017: Journal of the Medical Library Association: JMLA
https://www.readbyqxmd.com/read/28096737/implementation-of-evidence-on-the-nurse-patient-relationship-in-psychiatric-wards-through-a-mixed-method-design-study-protocol
#12
Antonio R Moreno-Poyato, Pilar Delgado-Hito, Raquel Suárez-Pérez, Juan M Leyva-Moral, Rosa Aceña-Domínguez, Regina Carreras-Salvador, Juan F Roldán-Merino, Teresa Lluch-Canut, Pilar Montesó-Curto
BACKGROUND: Psychiatric nurses are aware of the importance of the therapeutic relationship in psychiatric units. Nevertheless, a review of the scientific evidence indicates that theoretical knowledge alone is insufficient to establish an adequate therapeutic alliance. Therefore, strategies are required to promote changes to enhance the establishment of the working relationship. The aims of the study are to generate changes in how nurses establish the therapeutic relationship in acute psychiatric units, based on participative action research and to evaluate the effectiveness of the implementation of evidence through this method...
2017: BMC Nursing
https://www.readbyqxmd.com/read/28095844/barriers-and-facilitators-of-help-seeking-among-unemployed-persons-with-mental-health-problems-a-qualitative-study
#13
Tobias Staiger, Tamara Waldmann, Nicolas Rüsch, Silvia Krumm
BACKGROUND: Unemployed people with mental health problems often do not use mental health services and therefore do not benefit from available therapies. As unemployed individuals outside the healthcare system are a hard-to-reach group, barriers to and facilitators of mental health service use are poorly understood. The purpose of this study was to identify barriers to and facilitators of help-seeking and service use based on experiences of unemployed people with mental health problems...
January 17, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28095778/the-pangenome-of-antarctic-pseudoalteromonas-bacteria-evolutionary-and-functional-insights
#14
Emanuele Bosi, Marco Fondi, Valerio Orlandini, Elena Perrin, Isabel Maida, Donatella de Pascale, Maria Luisa Tutino, Ermenegilda Parrilli, Angelina Lo Giudice, Alain Filloux, Renato Fani
BACKGROUND: Pseudoalteromonas is a genus of ubiquitous marine bacteria used as model organisms to study the biological mechanisms involved in the adaptation to cold conditions. A remarkable feature shared by these bacteria is their ability to produce secondary metabolites with a strong antimicrobial and antitumor activity. Despite their biotechnological relevance, representatives of this genus are still lacking (with few exceptions) an extensive genomic characterization, including features involved in the evolution of secondary metabolites production...
January 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28095624/-it-s-good-enough-swedish-general-dental-practitioners-on-reasons-for-accepting-sub-standard-root-filling-quality
#15
L Dahlström, O Lindwall, H Rystedt, C Reit
AIM: The concept of "good enough" is central and necessary in the assessment of root filling quality. The aim was to explore the concept by analysing reasons and arguments for the acceptance or rejection of substandard root filling quality as reported by GDPs in Sweden. METHODOLOGY: The study was designed as a qualitative and exploratory study based on seven videotaped focus group interviews analysed by means of qualitative content analysis. Thirty-three general dental practitioners (GDPs) employed in the Public Dental Health Service in Gothenburg, Sweden, participated (4-6 GDPs/interview)...
January 17, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28095034/self-reported-driving-under-the-influence-of-alcohol-and-cannabis-among-ontario-students-associations-with-graduated-licensing-risk-taking-and-substance-abuse
#16
Steven Cook, Danielle Shank, Tara Bruno, Nigel E Turner, Robert E Mann
OBJECTIVE: This paper describes the patterns of self-reported driving under the influence of alcohol (DUIA) and driving under the influence of cannabis (DUIC) among licenced Ontario students in 2009, and examines their associations with graduated licencing, risk taking, and substance use problems for understanding DUIA and DUIC behaviours. Ontario's graduated licencing system requires new drivers to hold a G1 license for a minimum of 8 months, and a G2 licence for a minimum of 12 months before a full and unrestricted G license can be obtained...
January 17, 2017: Traffic Injury Prevention
https://www.readbyqxmd.com/read/28092013/sharpening-the-cutting-edge-additional-considerations-for-the-uk-debates-on-embryonic-interventions-for-mitochondrial-diseases
#17
Erica Haimes, Ken Taylor
In October 2015 the UK enacted legislation to permit the clinical use of two cutting edge germline-altering, IVF-based embryonic techniques: pronuclear transfer and maternal spindle transfer (PNT and MST). The aim is to use these techniques to prevent the maternal transmission of serious mitochondrial diseases. Major claims have been made about the quality of the debates that preceded this legislation and the significance of those debates for UK decision-making on other biotechnologies, as well as for other countries considering similar legislation...
December 2017: Life Sciences, Society and Policy
https://www.readbyqxmd.com/read/28090248/just-change-the-channel-studying-effects-of-age-on-emotion-regulation-using-a-tv-watching-paradigm
#18
Molly Sands, Adam Garbacz, Derek M Isaacowitz
Older adults are theorized to benefit from proactive forms of emotion regulation that allow them to avoid negative stimuli (Charles, 2010). To test this, we examined choices as a form of emotion regulation. In two studies investigating age differences, participants selected affective stimuli using a cable television interface, while choices and mood were recorded. In lab-based Study 1, older adults spent more time watching neutral channels, but younger adults spent more time watching positive ones. Older adults also watched more low-arousal content, while younger adults watched more high-arousal content...
November 2016: Social Psychological and Personality Science
https://www.readbyqxmd.com/read/28088294/suitability-of-small-diagnostic-peripheral-blood-samples-for-cell-therapy-studies
#19
Coralea Stephanou, Panayiota Papasavva, Myria Zachariou, Petros Patsali, Marilena Epitropou, Petros Ladas, Ruba Al-Abdulla, Soteroulla Christou, Michael N Antoniou, Carsten W Lederer, Marina Kleanthous
BACKGROUND AIMS: Primary hematopoietic stem and progenitor cells (HSPCs) are key components of cell-based therapies for blood disorders and are thus the authentic substrate for related research. We propose that ubiquitous small-volume diagnostic samples represent a readily available and as yet untapped resource of primary patient-derived cells for cell- and gene-therapy studies. METHODS: In the present study we compare isolation and storage methods for HSPCs from normal and thalassemic small-volume blood samples, considering genotype, density-gradient versus lysis-based cell isolation and cryostorage media with different serum contents...
February 2017: Cytotherapy
https://www.readbyqxmd.com/read/28087602/novel-application-of-3d-contrast-enhanced-cmr-to-define-fibrotic-structure-of-the-human-sinoatrial-node-in-vivo
#20
Thomas A Csepe, Jichao Zhao, Lidiya V Sul, Yufeng Wang, Brian J Hansen, Ning Li, Anthony J Ignozzi, Anna Bratasz, Kimerly A Powell, Ahmet Kilic, Peter J Mohler, Paul M L Janssen, John D Hummel, Orlando P Simonetti, Vadim V Fedorov
AIMS: The adult human sinoatrial node (SAN) has a specialized fibrotic intramural structure (35-55% fibrotic tissue) that provides mechanical and electrical protection from the surrounding atria. We hypothesize that late gadolinium-enhanced cardiovascular magnetic resonance (LGE-CMR) can be applied to define the fibrotic human SAN structure in vivo. METHODS AND RESULTS: LGE-CMR atrial scans of healthy volunteers (n olu, 23-52 y.o.) using a 3 Tesla magnetic resonance imaging system with a spatial resolution of 1...
January 13, 2017: European Heart Journal Cardiovascular Imaging
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