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Stimulation for IVF

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https://www.readbyqxmd.com/read/28796038/clomiphene-citrate-is-associated-with-favorable-cycle-characteristics-but-impaired-outcomes-of-obese-women-with-polycystic-ovarian-syndrome-undergoing-ovarian-stimulation-for-in-vitro-fertilization
#1
Shutian Jiang, Yanping Kuang
The aim of this study was to explore the effect of clomiphene citrate (CC) on the cycle characteristics and outcomes of obese women with polycystic ovarian syndrome (PCOS) undergoing ovarian stimulation for in vitro fertilization (IVF).This is a retrospective cohort study, and it was conducted at the tertiary-care academic medical center.This study included 174 obese PCOS patients undergoing IVF.In the study group (n = 90), CC and human menopausal gonadotropin (HMG) were administered simultaneously beginning on cycle day 3, while in control group (n = 84) HMG was used only...
August 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28795044/n-glycoproteomic-analysis-of-human-follicular-fluid-during-natural-and-stimulated-cycles-in-patients-undergoing-in-vitro-fertilization
#2
Hee-Joung Lim, Ae Eun Seok, Jiyou Han, Jiyeong Lee, Sungeun Lee, Hee-Gyoo Kang, Byung Heun Cha, Yunseok Yang
OBJECTIVE: Hyperstimulation methods are broadly used for in vitro fertilization (IVF) in patients with infertility; however, the side effects associated with these therapies, such as ovarian hyperstimulation syndrome (OHSS), have not been well studied. N-glycoproteomes are subproteomes used for the remote sensing of ovarian stimulation in follicular growth. Glycoproteomic variation in human follicular fluid (hFF) has not been evaluated. In this study, we aimed to identify and quantify the glycoproteomes and N-glycoproteins (N-GPs) in natural and stimulated hFF using label-free nano-liquid chromatography/electrospray ionization-quad time-of-flight mass spectrometry...
June 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28791974/experience-with-the-bonanno-catheter-in-the-management-of-ohss-from-ivf-et-cycles
#3
J E Okohue, V K Oriji, J I Ikimalo
OBJECTIVE: To document our experience with the use of the Bonanno catheter as a closed abdominal drain for OHSS Methods: A retrospective study of all IVF embryo transfer (ET) treatment cycles carried out between May 2006 and April 2009 at a dedicated IVF centre. Case notes of patients with OHSS were retrieved and the outcome of the continuous closed abdominal drain with Bonanno catheter documented. RESULT: Within the period under review, 234 patients had controlled ovarian stimulation with ultrasound guided egg retrieval...
July 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/28776117/gnrh-antagonist-administered-twice-the-day-before-hcg-trigger-combined-with-a-step-down-protocol-may-prevent-ohss-in-ivf-icsi-antagonist-cycles-at-risk-for-ohss-without-affecting-the-reproductive-outcomes-a-prospective-randomized-control-trial
#4
Yannis Prapas, Konstantinos Ravanos, Stamatios Petousis, Yannis Panagiotidis, Achilleas Papatheodorou, Chrysoula Margioula-Siarkou, Assunta Iuliano, Giuseppe Gullo, Nikos Prapas
PURPOSE: The purpose this study is to investigate whether a double antagonist dose (0.25 mg/12 h) administered the day before hCG trigger is effective in preventing ovarian hyperstimulation syndrome (OHSS) in GnRH antagonist IVF/intracytoplasmic sperm injection (ICSI) cycles at risk for OHSS. METHODS: This is a prospective randomized control study, conducted from November 2012 to January 2016. A total of 194 patients undergoing a IVF/ICSI GnRH antagonist cycle that were at risk of OHSS and chose to proceed with embryo transfer and avoid cycle cancellation or embryo cryopreservation were allocated into two groups...
August 3, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28774548/safety-and-efficiency-of-oocyte-vitrification
#5
REVIEW
Neelke De Munck, Gábor Vajta
As the oocyte is the starting point for a new life, artificial reproductive technology (ART) techniques should not affect the (ultra) structural and functional integrity, or the developmental competence. Oocyte vitrification -one of the most significant achievements in human ART during the past decade- should therefore be a safe and efficient technique. This review discusses the principles and developments of the existing and future techniques, applications possibilities and safety concerns. The broad range of vitrification media and devices that are currently available, show differences in their effects on the oocyte ultrastructure and preimplantation development...
August 1, 2017: Cryobiology
https://www.readbyqxmd.com/read/28750299/prolonged-activation-of-the-coagulation-system-during-in-vitro-fertilization-cycles
#6
Yoni Cohen, Togas Tulandi, Benny Almog, Eyal Zohav, Varda Deutsch, Ariel Many, Ami Amit, Aviad Cohen
OBJECTIVE: To investigate coagulation system changes during an in-vitro fertilization (IVF) cycle using Thromboelastogram (TEG) that enables analysis of the elastic properties of whole blood samples and provides a global assessment of the hemostatic function. STUDY DESIGN: A prospective study. TEG indices were evaluated in 23 women who underwent controlled ovarian stimulation for IVF at four points in time: 1. At the beginning of the cycle (corresponding to the lowest levels of E2), 2...
July 17, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28738699/caspase-3-gene-expression-in-human-luteinized-granulosa-cells-is-inversely-correlated-with-the-number-of-oocytes-retrieved-after-controlled-ovarian-stimulation
#7
Verônica N Lobach, Maíra Casalechi, Cynthia Dela Cruz, Maria T Pereira, Helen L Del Puerto, Fernando M Reis
Granulosa cells control oocyte maturation through paracrine signalling and changes to the microenvironment around the oocyte. Apoptosis occurs as a physiological mechanism of granulosa cell renewal, but how it relates with the ovarian response to induced ovulation is still unclear. Therefore, this study evaluated apoptosis-related gene expression levels in granulosa cells of patients undergoing controlled ovarian stimulation. We enrolled prospectively 59 consecutive IVF patients referred to a tertiary academic hospital for couple infertility treatment...
July 25, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28736153/in-vitro-study-of-gonadotrophin-signaling-pathways-in-human-granulosa-cells-in-relation-to-progesterone-receptor-expression
#8
Soledad Henríquez, Paulina Kohen, Alex Muñoz, Ana Godoy, Felipe Orge, Jerome F Strauss, Luigi Devoto
In humans, data on gonadotrophin-activated (LH, HCG and FSH) progesterone receptor expression and signalling pathways involved in matrix metalloproteinases (MMPs) expression presumably linked to the follicle rupture, are limited. Our hypothesis is LH, HCG and FSH increase progesterone receptor expression in granulosa cells through different signalling pathways, leading to an increased expression of ADAMTS-1 and MMP3/10, which may mediate follicular rupture through the transcription factor, HIF1A. Human granulosa cells were isolated from follicular aspirates obtained from 22 healthy women participating in our IVF programme for male-factor infertility...
June 23, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28721178/global-reproductive-health-why-do-we-persist-in-neglecting-the-undeniable-problem-of-childlessness-in-resource-poor-countries
#9
EDITORIAL
W Ombelet, J Goossens
The consequences of involuntary childlessness in developing countries create more wide-ranging societal problems compared to Western societies, particularly for women. Negative psychosocial and economical consequences for childless couples are often severe and underestimated by the local and international society. Infertility treatment is often limited to certain procedures and certain costumers. Most common arguments against supporting infertility care in resource-poor countries are the "overpopulation" and the "limited resources" argument, but they totally neglect the reproductive rights and systematic exclusion of millions of women from the right to reproduce...
March 2017: Facts, Views & Vision in ObGyn
https://www.readbyqxmd.com/read/28715965/dual-triggering-with-gonadotropin-releasing-hormone-agonist-and-standard-dose-human-chorionic-gonadotropin-in-patients-with-a-high-immature-oocyte-rate
#10
Alberta Maria Fabris, M Cruz, V Legidos, C Iglesias, M Muñoz, J A García-Velasco
In in vitro fertilization (IVF) cycles, some patients show a high rate of immature oocytes retrieved after controlled ovarian stimulation. In vitro oocyte maturation is an experimental technique, with poorer results than conventional IVF. For this reason, improving in vivo maturation could meliorate the reproductive outcome of these patients. We performed a retrospective, not interventional, study analyzing the difference in the number and percentage of mature oocytes retrieved in patients with more than 50% immature oocytes in a previous IVF cycle triggered with human chorionic gonadotropin (hCG) compared to the number and rate of mature oocytes retrieved in subsequent cycles, triggered with both gonadotropin-releasing hormone agonist (GnRH-a) and hCG...
August 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28706366/gnrh-antagonist-cetrorelix-administration-before-hcg-for-protection-of-ovarian-hyperstimulation-syndrome
#11
Sherif A Hebisha, Banan A Aboelazm, H N Sallam
OBJECTIVE: Studying the effect of GnRH antagonist administration on the day of hCG to cases of IVF/ICSI with estradiol level above 5000 ng/dl for protection of ovarian hyperstimulation syndrome. DESIGN: Prospective study. MATERIALS AND METHODS: Sixty patients undergoing controlled hyperstimulation COH, for IVF/ICSI using long agonist and E2 level on the day of hCG, are above 5000 ng/dl, 52 patients received single dose of cetrorelix 0.25 mg on the day of hCG, and 8 patients received two doses of 0...
August 2017: Journal of Obstetrics and Gynaecology of India
https://www.readbyqxmd.com/read/28695963/differential-regulation-of-cumulus-cell-transcription-during-oocyte-maturation-in-vivo-and-in-vitro
#12
Giovanni Coticchio, Libby Ophir, Yuval Yung, Micha Baum, Mariabeatrice Dal Canto, Mario Mignini-Renzini, Fausta Brambillasca, Rubens Fadini, Ariel Hourvitz
Differences in cumulus cell gene expression after oocyte maturation in vitro (IVM) or in vivo have been described in previous studies. However, the possible impact of follicle stage on gene expression deregulation during human oocyte IVM remains unknown. Expression of selected genes of interest was compared in cumulus cell of three classes of human cumulus cell-oocyte complexes (COCs): a) COCs derived from human chorionic gonadotropin (hCG)-triggered IVM cycles, collected at the germinal vesicle (GV) stage from mid-sized follicles (4-12 mm) and matured in vitro (IVM-GV); b) COCs derived from hCG-triggered IVM cycles, collected from mid-sized follicles (4-12 mm) and matured in vivo (IVM-MII); c) COCs derived from controlled ovarian stimulation in vitro fertilization (IVF) cycles, collected from large/preovulatory follicles and matured in vivo (IVF-MII)...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/28674502/treatment-of-abnormal-vaginal-microbiota-before-frozen-embryo-transfer-case-report-and-minireview-to-discuss-the-longitudinal-treatment-efficacy-of-oral-clindamycin
#13
Thor Haahr, Helle O Elbaek, Rita J Laursen, Birgit Alsbjerg, Jørgen S Jensen, Peter Humaidan
Abnormal vaginal microbiota (AVM) or bacterial vaginosis (BV) might negatively impact reproductive outcomes of in vitro fertilization (IVF). However, before randomized controlled trials are initiated to investigate cause and effect, it is necessary to establish the optimal treatment for AVM. Metronidazole seems ineffective to treat the biofilm in AVM; thus, clindamycin could be suggested as a relevant antibiotic agent for future intervention based studies. In the present case report, we present the first longitudinal follow-up of the vaginal microbiota with molecular methods during and after oral clindamycin treatment...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28670425/vitrification-of-human-germinal-vesicle-oocytes-before-or-after-in-vitro-maturation
#14
Evangelia Kasapi, Byron Asimakopoulos, Katerina Chatzimeletiou, Stamatios Petousis, Yannis Panagiotidis, Nikos Prapas, Nikos Nikolettos
BACKGROUND: The use of immature oocytes derived from stimulated cycles could be of great importance, particularly for urgent fertility preservation cases. The current study aimed to determine whether in vitro maturation (IVM) was more successful before or after vitrification of these oocytes. MATERIALS AND METHODS: This prospective study was performed in a private in vitro fertilization (IVF) center. We collected 318 germinal vesicle (GV) oocytes from 104 stimulated oocyte donation cycles...
July 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28670424/a-comparison-of-outcomes-from-in-vitro-fertilization-cycles-stimulated-with-follicle-stimulating-hormone-plus-either-recombinant-luteinizing-hormone-or-human-menopausal-gonadotropins-in-subjects-treated-with-long-gonadotropin-releasing-hormone-agonist-protocols
#15
Nathalie Bleau, Mohammed Agdi, WeonYoung Son, SeangLin Tan, Michael H Dahan
BACKGROUND: This study compared rates of pregnancy and in vitro fertilization (IVF) parameters in subjects stimulated with follicle stimulating hormone (FSH) plus either recombinant human luteinizing hormone (r-LH) or human menopausal gonadotropin (hMG) in a long gonadotropin releasing hormone (GnRH) agonist IVF protocol. MATERIALS AND METHODS: This cohort study enrolled patients who underwent IVF stimu- lation with a long GnRH agonist protocol and received FSH plus r-LH or hMG...
July 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28666459/towards-a-better-understanding-of-preimplantation-genetic-screening-for-aneuploidy-insights-from-a-virtual-trial-for-women-under-the-age-of-40-when-transferring-embryos-one-at-a-time
#16
Paul N Scriven
BACKGROUND: The aim of this theoretical study is to explore the cost-effectiveness of aneuploidy screening in a UK setting for every woman aged under the age of 40 years when fresh and vitrified-warmed embryos are transferred one at a time in a first full cycle of assisted conception. METHODS: It is envisaged that a 24-chromosome genetic test for aneuploidy could be used to exclude embryos with an abnormal test result from transfer, or used only to rank embryos with the highest potential to be viable; the effect on cumulative outcome is assessed...
June 30, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28645286/the-myths-surrounding-mild-stimulation-in-vitro-fertilization-ivf
#17
REVIEW
Raoul Orvieto, Valeria Stella Vanni, Norbert Gleicher
So-called mild controlled ovarian hyperstimulation (mCOH) has in recent years increased in popularity, claiming to be safer and more patient-friendly, while also improving in vitro fertilization (IVF) outcomes. We here challenge the International Society for Mild Approaches in Assisted Reproduction (ISMAAR) definition of mild stimulation, and especially address four fundamental issues, where our review found conventional COH (cCOH) advantageous over mCOH. They are: prevalence of severe ovarian hyperstimulation syndrome (OHSS), oocyte/embryo quality, pregnancy/live birth rates, and cost...
June 24, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28639180/initial-maternal-serum-human-chorionic-gonadotropin-levels-in-pregnancies-achieved-after-assisted-reproductive-technology-are-higher-after-preimplantation-genetic-screening-and-after-frozen-embryo-transfer-a-retrospective-cohort
#18
Elie Hobeika, Sonali Singh, Shaveta Malik, Eric S Knochenhauer, Michael L Traub
PURPOSE: Few published articles have compared initial hCG values across all different types of ART cycles, including cycles with fresh or frozen embryo transfer. No articles have compared initial hCG values in cycles utilizing preimplantation genetic screening (PGS). The purpose of this study is to compare initial hCG values after fresh embryo transfer, frozen embryo transfer, and after PGS. METHODS: This was a single-center retrospective cohort study at an academically affiliated private IVF center...
June 21, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28635413/the-high-responder-a-review-of-pathophysiology-and-outcomes-during-ivf-treatment
#19
Mariano Mascarenhas, Adam H Balen
This review attempts to summarize the known literature on high responders to ovarian stimulation during assisted reproductive techniques (ART). Response to gonadotrophins is subject to significant interindividual and intercycle variation, thus carrying a risk of high response or poor response to ovarian stimulation regimens. The main risk for high responders is the development of ovarian hyperstimulation syndrome (OHSS) which is associated with significant morbidity. Hence, the definition of high responders in the literature has primarily focussed on risk factors for OHSS...
September 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28632990/shortening-gametes-co-incubation-time-improves-live-birth-rate-for-couples-with-a-history-of-fragmented-embryos
#20
Anne Le Bras, Laetitia Hesters, Vanessa Gallot, Cathie Tallet, Gerard Tachdjian, Nelly Frydman
Short gamete co-incubation (SGCO) consists in decreasing the duration of contact between oocytes and sperm from the standard overnight insemination (SOI) toward 2 hours. However, the effectiveness of this technique to improve in vitro fertilization and embryo transfer (IVF-ET) outcomes remains controversial. Our study was designed to evaluate the efficiency of SGCO in a poor prognosis population with a history of fragmented embryos defined by the presence of at least 50% of the embryos with more than 25% of cytoplasmic fragments...
June 20, 2017: Systems Biology in Reproductive Medicine
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