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https://www.readbyqxmd.com/read/28966348/x-rays-from-the-location-of-the-double-humped-transient-asassn-15lh
#1
R Margutti, B D Metzger, R Chornock, D Milisavljevic, E Berger, P K Blanchard, C Guidorzi, G Migliori, A Kamble, R Lunnan, M Nicholl, D L Coppejans, S Dall'Osso, M R Drout, R Perna, B Sbarufatti
We present the detection of persistent soft X-ray radiation with Lx ~ 10(41)-10(42) erg s(-1) at the location of the extremely luminous, double-humped transient ASASSN-15lh as revealed by Chandra and Swift. We interpret this finding in the context of observations from our multiwavelength campaign, which revealed the presence of weak narrow nebular emission features from the host-galaxy nucleus and clear differences with respect to superluminous supernova optical spectra. Significant UV flux variability on short timescales detected at the time of the rebrightening disfavors the shock interaction scenario as the source of energy powering the long-lived UV emission, while deep radio limits exclude the presence of relativistic jets propagating into a low-density environment...
February 10, 2017: Astrophysical Journal
https://www.readbyqxmd.com/read/28963605/sensitive-electrochemical-detection-of-dna-damage-based-on-in-situ-double-strand-growth-via-hybridization-chain-reaction
#2
Misha Liu, Jinjin Xu, Fan Yang, Yifan Gu, Huan Chen, Ying Wang, Fengting Li
Detection of DNA damage caused by ∙OH or radiation has led to rapidly growing interest in the fields of drug development, biochemistry, clinic diagnostics, and environmental evaluation. Electrochemical methods have been applied for DNA damage detection because of their fast and sensitive response. However, most of the electrochemical methods for DNA damage detection commonly require immobilization of the strands on the electrode surface. In the present work, sensitive electrochemical monitoring of DNA damage was realized successfully on the basis of in situ DNA chain growth by use of a hybridization chain reaction (HCR) technique...
September 29, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28949137/selective-excitation-of-cyanophenylalanine-fluorophores-for-multi-site-binding-studies
#3
Natalie R Fetto, Wenqiang Cao, Ian S Wallace, Matthew J Tucker
Recently, it has been shown that nitrile derivatized phenylalanines possess distinct fluorescent properties depending on the position of the cyano- group within the aromatic ring. These fluorophores have potential as probes for studying protein dynamics due to their sensitivity to local environment. Herein, we demonstrate that 2-cyanophenylalanine (Phe2CN) and Phe4CN can independently monitor multiple sites during the Ca2+ dependent binding of a skeletal muscle myosin light chain kinase (MLCK) peptide fragment to the protein calmodulin (CaM)...
September 26, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28936657/whole-mount-rna-fish-on-ovules-and-developing-seeds
#4
Andrea Bleckmann, Thomas Dresselhaus
A key element to understand developmental and reproductive processes like germline development, double fertilization, and embryogenesis is the study of cell-specific gene expression patterns which is best analyzed by RNA in situ hybridization. Different visualization techniques have been established to mark either the region of mRNA production (using the classical chromogenic detection system) or the specific localization of mRNAs (using fluorescent labeled probes). In this chapter, we describe and compare whole mount RNA in situ hybridization techniques on ovules and young developing seeds from Arabidopsis thaliana using three different detection systems...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28924230/two-step-excitation-in-hot-atomic-sodium-vapor
#5
Bernd Docters, Jörg Wrachtrup, Ilja Gerhardt
A two step excitation scheme in hot atomic sodium vapor is experimentally investigated. The observed effects reflect a coupling between the 3(2)S, 3(2)P and the 3(2)D states. We present the relative dependence on detuning of the two utilized lasers around λ = 589 nm and 819 nm. Unlike expected, we achieve a higher detuning dependence of the probe and the coupling laser by a factor of approximately three. The presented work aimed for a Rydberg excitation and quantum light storage. Such schemes are usually implemented with a red laser on the D-line transition and a coupling laser of shorter (typically blue) wavelength...
September 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28920423/self-organization-of-ions-at-the-interface-between-graphene-and-ionic-liquid-deme-tfsi
#6
Guangliang Hu, Gaind P Pandey, Qingfeng Liu, Radhika S Anaredy, Chunrui Ma, Ming Liu, Jun Li, Scott K Shaw, Judy Wu
Electrochemical effects manifest as nonlinear responses to an applied electric field in electrochemical devices, and are linked intimately to the molecular orientation of ions in the electric double layer (EDL). Herein, we probe the origin of the electrochemical effect using a double-gate graphene field effect transistor (GFET) of ionic liquid N,N-diethyl-N-(2-methoxyethyl)-N-methylammonium bis(trifluoromethylsulfonyl)imide (DEME-TFSI) top-gate, paired with a ferroelectric Pb0.92La0.08Zr0.52Ti0.48O3 (PLZT) back-gate of compatible gating efficiency...
October 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28877115/quantitation-of-24-hour-moisturization-by-electrical-measurements-of-skin-hydration
#7
R Randall Wickett, Bronson Damjanovic
PURPOSE: The purpose of this study was to quantify the effects of several moisturizers on hydration of the stratum corneum by measuring their effect on electrical conductance over a 24-hour period. DESIGN: Double-blind, randomized controlled trial. SUBJECTS AND SETTING: Twenty-five healthy female volunteers aged 18 to 65 years with dry skin on the lower legs and no other known dermatologic pathology participated in the study. Additional exclusion criteria were pregnant or taking anti-inflammatory steroids...
September 2017: Journal of Wound, Ostomy, and Continence Nursing
https://www.readbyqxmd.com/read/28864184/reduced-graphene-oxide-as-a-resonance-light-scattering-probe-for-thrombin-detection-using-dual-aptamer-based-dsdna
#8
Feng Chen, Yi Liu, Rong Liao, Hang Gong, Chunyan Chen, Xiaoming Chen, Changqun Cai
This paper describes a reduced graphene oxide (RGO)-based resonance light-scattering (RLS) method for thrombin detection by using double strand DNA (dsDNA) as a binding element. dsDNA is obtained by hybridizing DNA1 and DNA2, which respectively consist of one aptamer of thrombin and the complementary strand of the other aptamer of thrombin. When thrombin is added, the specific binding of two aptamers to thrombin results in a complex (DNA1-thrombin-DNA2) and triggers the release of the complementary strand of two aptamers from dsDNA...
September 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28725027/strong-coherent-light-amplification-with-double-electromagnetically-induced-transparency-coherences
#9
Dan Wang, Chao Liu, Changshun Xiao, Junxiang Zhang, Hessa M M Alotaibi, Barry C Sanders, Li-Gang Wang, Shiyao Zhu
We experimentally demonstrate coherent amplification of probe field in a tripod-type atoms driven by strong coupling, signal and weak probe fields. We suppress linear and nonlinear atomic absorptions for resonant and near resonant probe via double electromagnetically induced transparency (DEIT). Combining these advantages of suppressed absorption along with temperature- or atomic-density-controlled transfer of population(ToP) between hyperfine ground states, we can induce near-resonant amplification of probe through stimulated Raman scattering(SRS) pumped by low-intensity signal field...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28706354/intensities-of-incident-and-transmitted-ultraviolet-a-rays-through-gafchromic-films
#10
Toshizo Katsuda, Rumi Gotanda, Tatsuhiro Gotanda, Takuya Akagawa, Nobuyoshi Tanki, Tadao Kuwano, Atsushi Noguchi, Kouichi Yabunaka
Gafchromic films have been applied to X-ray dosimetry in diagnostic radiology. To correct nonuniformity errors in Gafchromic films, X-rays in the double-exposure technique can be replaced with ultraviolet (UV)-A rays. Intensities of the incident and transmitted UV-A rays were measured. However, it is unclear whether the chemical color change of Gafchromic films affects the UV-A transmission intensity. Gafchromic EBT3 films were suitable to be used in this study because non-UV protection layers are present on both sides of the film...
April 2017: Journal of Medical Physics
https://www.readbyqxmd.com/read/28700536/fully-implantable-battery-free-wireless-optoelectronic-devices-for-spinal-optogenetics
#11
Vijay K Samineni, Jangyeol Yoon, Kaitlyn E Crawford, Yu Ra Jeong, Kajanna C McKenzie, Gunchul Shin, Zhaoqian Xie, Saranya S Sundaram, Yuhang Li, Min Young Yang, Jeonghyun Kim, Di Wu, Yeguang Xue, Xue Feng, Yonggang Huang, Aaron D Mickle, Anthony Banks, Jeong Sook Ha, Judith P Golden, John A Rogers, Robert W Gereau
The advent of optogenetic tools has allowed unprecedented insights into the organization of neuronal networks. Although recently developed technologies have enabled implementation of optogenetics for studies of brain function in freely moving, untethered animals, wireless powering and device durability pose challenges in studies of spinal cord circuits where dynamic, multidimensional motions against hard and soft surrounding tissues can lead to device degradation. We demonstrate here a fully implantable optoelectronic device powered by near-field wireless communication technology, with a thin and flexible open architecture that provides excellent mechanical durability, robust sealing against biofluid penetration and fidelity in wireless activation, thereby allowing for long-term optical stimulation of the spinal cord without constraint on the natural behaviors of the animals...
November 2017: Pain
https://www.readbyqxmd.com/read/28668731/determination-of-thorium-iv-using-isophthalaldehyde-tetrapyrrole-as-probe-by-resonance-light-scattering-second-order-scattering-and-frequency-doubling-scattering-spectra
#12
Jiao Wang, Jinhua Xue, Xilin Xiao, Li Xu, Min Jiang, Pengcheng Peng, Lifu Liao
The coordination reaction of thorium (IV) with a ditopic bidentate ligand to form supramolecular polymer was studied by resonance light scattering (RLS) spectra, second-order scattering (SOS) spectra and frequency-doubling scattering (FDS) spectra, respectively. The ditopic bidentate ligand is isophthalaldehyde-tetrapyrrole (IPTP). It was synthesized through a condensation reaction of isophthalaldehyde with pyrrole. The formation of supramolecular polymer results in remarkable intensity enhancements of the three light scattering signals...
June 27, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28579916/kilonovae
#13
REVIEW
Brian D Metzger
The mergers of double neutron star (NS-NS) and black hole (BH)-NS binaries are promising gravitational wave (GW) sources for Advanced LIGO and future GW detectors. The neutron-rich ejecta from such merger events undergoes rapid neutron capture (r-process) nucleosynthesis, enriching our Galaxy with rare heavy elements like gold and platinum. The radioactive decay of these unstable nuclei also powers a rapidly evolving, supernova-like transient known as a "kilonova" (also known as "macronova"). Kilonovae are an approximately isotropic electromagnetic counterpart to the GW signal, which also provides a unique and direct probe of an important, if not dominant, r-process site...
2017: Living Reviews in Relativity
https://www.readbyqxmd.com/read/28524354/an-aggregating-amphiphilic-squaraine-a-light-up-probe-that-discriminates-parallel-g-quadruplexes
#14
Vincenzo Grande, Filippo Doria, Mauro Freccero, Frank Würthner
G-quadruplexes (G4s) are peculiar DNA or RNA tertiary structures that are involved in the regulation of many biological events within mammalian cells, bacteria, and viruses. Although their role as versatile therapeutic targets has been emphasized for 35 years, G4 selectivity over ubiquitous double-stranded DNA/RNA, as well as G4 differentiation by small molecules, still remains challenging. Here, a new amphiphilic dicyanovinyl-substituted squaraine, SQgl, is reported to act as an NIR fluorescent light-up probe discriminating an extensive panel of parallel G4s while it is non-fluorescent in the aggregated state...
May 19, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28477124/chip-technique-to-study-protein-dynamics-at-defined-dna-double-strand-breaks
#15
Jie Wen, Patrick Concannon
Information about the timing of appearance and composition of protein aggregates, termed foci, that arise in eukaryotic cells at sites of DNA double strand breaks (DSBs) has been mainly obtained through immunostaining and thus is limited by the resolution of light microscopy and the availability of appropriate antibodies. In this chapter, we describe a system using direct protein transduction of homing endonuclease, I-PpoI, into human cells to generate site-specific DSBs, allowing for detection of target proteins using chromatin immunoprecipitation (ChIP), enabling molecular probing of the cellular response to a DNA DSB...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28339208/doubly-resonant-photonic-antenna-for-single-infrared-quantum-dot-imaging-at-telecommunication-wavelengths
#16
Zhihua Xie, Yannick Lefier, Miguel Angel Suarez, Mathieu Mivelle, Roland Salut, Jean-Marc Merolla, Thierry Grosjean
Colloidal quantum dots (CQDs) have drawn strong interest in the past for their high prospects in scientific, medical, and industrial applications. However, the full characterization of these quantum emitters is currently restricted to the visible wavelengths, and it remains a key challenge to optically probe single CQDs operating in the infrared spectral domain, which is targeted by a growing number of applications. Here, we report the first experimental detection and imaging at room temperature of single infrared CQDs operating at telecommunication wavelengths...
March 24, 2017: Nano Letters
https://www.readbyqxmd.com/read/28289094/intersubunit-distances-in-full-length-dimeric-bacterial-phytochrome-agp1-as-measured-by-pulsed-electron-electron-double-resonance-peldor-between-different-spin-label-positions-remain-unchanged-upon-photoconversion
#17
Sylwia Kacprzak, Ibrahim Njimona, Anja Renz, Juan Feng, Edward Reijerse, Wolfgang Lubitz, Norbert Krauss, Patrick Scheerer, Soshichiro Nagano, Tilman Lamparter, Stefan Weber
Bacterial phytochromes are dimeric light-regulated histidine kinases that convert red light into signaling events. Light absorption by the N-terminal photosensory core module (PCM) causes the proteins to switch between two spectrally distinct forms, Pr and Pfr, thus resulting in a conformational change that modulates the C-terminal histidine kinase region. To provide further insights into structural details of photoactivation, we investigated the full-length Agp1 bacteriophytochrome from the soil bacterium Agrobacterium fabrum using a combined spectroscopic and modeling approach...
May 5, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28211680/two-way-gold-nanoparticle-label-free-sensing-of-specific-sequence-and-small-molecule-targets-using-switchable-concatemers
#18
Longjiao Zhu, Xiangli Shao, Yunbo Luo, Kunlung Huang, Wentao Xu
A two-way colorimetric biosensor based on unmodified gold nanoparticles (GNPs) and a switchable double-stranded DNA (dsDNA) concatemer have been demonstrated. Two hairpin probes (H1 and H2) were first designed that provided the fuels to assemble the dsDNA concatemers via hybridization chain reaction (HCR). A functional hairpin (FH) was rationally designed to recognize the target sequences. All the hairpins contained a single-stranded DNA (ssDNA) loop and sticky end to prevent GNPs from salt-induced aggregation...
May 19, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28168258/spectroscopic-and-microscopic-investigations-of-tautomerization-in-porphycenes-condensed-phases-supersonic-jets-and-single-molecule-studies
#19
P Fita, L Grill, A Listkowski, H Piwoński, S Gawinkowski, M Pszona, J Sepioł, E Mengesha, T Kumagai, J Waluk
We describe various experimental approaches that have been used to obtain a detailed understanding of double hydrogen transfer in porphycene, a model system for intramolecular hydrogen bonding and tautomerism. The emerging picture is that of a multidimensional tautomerization coordinate, with several vibrational modes acting as reaction-promoters or inhibitors through anharmonic intermode coupling. Tunnelling processes, coherent in the case of isolated molecules and incoherent in condensed phases, are found to play a major role even at elevated temperatures...
February 7, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28058829/detecting-electric-dipoles-interaction-at-the-interface-of-ferroelectric-and-electrolyte-using-graphene-field-effect-transistors
#20
Chunrui Ma, Rongtao Lu, Guangliang Hu, Jinsheng Han, Ming Liu, Jun Li, Judy Wu
Graphene was inserted into the interface between electric dipole layers from DEME-TFSI ionic liquid (top-gate) and ferroelectric Pb0.92La0.08Zr0.52Ti0.48O3 (PLZT, back-gate) to probe the interface dipole-dipole interaction in response to DC and pulsed gate voltages. A highly complicated behavior of the interface dipole-dipole interaction has been revealed as a combination of electrostatic and electrochemical effects. The interfacial polar molecules in the DEME-TFSI electrical double layer are pinned with assistance from the PLZT back-gate in response to a DC top-gate pump, leading to strong nonlinear electrochemical behavior...
February 1, 2017: ACS Applied Materials & Interfaces
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