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Xiaoqun Zhang, Ioannis Mantas, Alexandra Alvarsson, Takashi Yoshitake, Mohammadreza Shariatgorji, Marcela Pereira, Anna Nilsson, Jan Kehr, Per E Andrén, Mark J Millan, Karima Chergui, Per Svenningsson
The trace amine-associated receptor 1 (TAAR1) is expressed by dopaminergic neurons, but the precise influence of trace amines upon their functional activity remains to be fully characterized. Here, we examined the regulation of tyrosine hydroxylase (TH) by tyramine and beta-phenylethylamine (β-PEA) compared to 3-iodothyronamine (T1 AM). Immunoblotting and amperometry were performed in dorsal striatal slices from wild-type (WT) and TAAR1 knockout (KO) mice. T1 AM increased TH phosphorylation at both Ser19 and Ser40 , actions that should promote functional activity of TH...
2018: Frontiers in Pharmacology
Iracema Caballero, Massimo Sammito, Claudia Millán, Andrey Lebedev, Nicolas Soler, Isabel Usón
ARCIMBOLDO solves the phase problem by combining the location of small model fragments using Phaser with density modification and autotracing using SHELXE. Mainly helical structures constitute favourable cases, which can be solved using polyalanine helical fragments as search models. Nevertheless, the solution of coiled-coil structures is often complicated by their anisotropic diffraction and apparent translational noncrystallographic symmetry. Long, straight helices have internal translational symmetry and their alignment in preferential directions gives rise to systematic overlap of Patterson vectors...
March 1, 2018: Acta Crystallographica. Section D, Structural Biology
Ginetta Collo, Laura Cavalleri, Federica Bono, Cristina Mora, Stefania Fedele, Roberto William Invernizzi, Massimo Gennarelli, Giovanna Piovani, Tilo Kunath, Mark J Millan, Emilio Merlo Pich, PierFranco Spano
The antiparkinsonian ropinirole and pramipexole are D3 receptor- (D3R-) preferring dopaminergic (DA) agonists used as adjunctive therapeutics for the treatment resistant depression (TRD). While the exact antidepressant mechanism of action remains uncertain, a role for D3R in the restoration of impaired neuroplasticity occurring in TRD has been proposed. Since D3R agonists are highly expressed on DA neurons in humans, we studied the effect of ropinirole and pramipexole on structural plasticity using a translational model of human-inducible pluripotent stem cells (hiPSCs)...
2018: Neural Plasticity
Fernando Bril, Laura Millán, Srilaxmi Kalavalpalli, Michael J McPhaul, Michael P Caulfield, Ibon Martinez-Arranz, Cristina Alonso, Pablo Ortiz Betes, José M Mato, Kenneth Cusi
AIMS: While several non-invasive clinical/metabolic scores have been designed to predict the presence of nonalcoholic steatohepatitis (NASH), none was specifically validated in patients with T2DM. We aimed to assess the utility of existing metabolomics scores to classify liver disease in patients with T2DM. MATERIALS AND METHODS: A total of 220 patients with T2DM were recruited. Patients underwent routine lab tests, a liver proton magnetic resonance spectroscopy (1 H-MRS), a 75-gram OGTT, and a liver biopsy if they had NAFLD by1 H-MRS...
March 12, 2018: Diabetes, Obesity & Metabolism
María Del Mar Roca-Rodríguez, María Carmen Ayala-Ortega, Ana Isabel Jiménez-Millán, María Concepción García Calzado, María Soledad Ruiz de Adana, Florentino Carral-San Laureano
INTRODUCTION: MODY diabetes encompasses heterogeneous group of monogenic forms of diabetes with low prevalence. It is not easily diagnosed because of the increase in obesity and family history of diabetes in the general population. PATIENTS AND METHODS: We present a clinical case with cardinal symptoms, diabetes,renal insufficiency with no acidosis and with a family history of diabetes and renal agenesis. RESULTS: Distinguishing MODY diabetes from DM1 and DM2 is very important to ensure optimal treatment, and because the risk of complications depends on each genetic defect...
March 7, 2018: Medicina Clínica
Alberto Ramírez Mata, César Millán Pacheco, José F Cruz Pérez, Martha Minjárez Sáenz, Beatriz E Baca
BACKGROUND: The cyclic-di-GMP (c-di-GMP) second messenger exemplifies a signaling system that regulates many bacterial behaviors of key importance; among them, c-di-GMP controls the transition between motile and sessile life-styles in bacteria. Cellular c-di-GMP levels in bacteria are regulated by the opposite enzymatic activities of diguanylate cyclases and phosphodiesterases, which are proteins that have GGDEF and EAL domains, respectively. Azospirillum is a genus of plant-growth-promoting bacteria, and members of this genus have beneficial effects in many agronomically and ecologically essential plants...
March 9, 2018: BMC Microbiology
Sandra M Londoño-Restrepo, Natalia Rincón-Londoño, Margarita Contreras-Padilla, Beatriz M Millan-Malo, Mario E Rodriguez-Garcia
This work is focused on the chemical, structural, morphological, thermal, IR vibrational, and pasting characterization of isolated white, yellow, and purple Arracacha starches from Colombia. Inductive couple plasma showed that these starches are rich in potassium. Scanning Electron Microscopy (SEM) images show that the starch granules are formed by ovoid fully filled Lego-like starch microparticles, the circular cross-section has a diameter between 9 and 15 μm and mayor axis between 20 and 30 μm. Each one of these ovoids is formed by irregular wedge-shaped 6 to 10 isolated starch granules with an average size between 4 and 12 μm...
March 6, 2018: International Journal of Biological Macromolecules
Carmen de Labra, Ana Maseda, Laura Lorenzo-López, Rocío López-López, Ana Buján, José L Rodríguez-Villamil, José Carlos Millán-Calenti
BACKGROUND: Frailty is a multidimensional clinical geriatric syndrome that may be reversed in its early stages. Most studies have paid attention to its physical or phenotypic boundaries, however, little is known about the social aspects surrounding this geriatric syndrome. The study examined the relationship between socio-demographic factors, social resources, quality of life and frailty in older adults. METHODS: This cross-sectional study included a representative sample (n = 749) of adults aged ≥65 years enrolled in forty-three senior centers located in North-West Spain...
March 7, 2018: BMC Geriatrics
Juan Girbés Borrás, Javier Escalada San Martín, Manel Mata Cases, Fernando Gomez-Peralta, Sara Artola Menéndez, Diego Fernández García, Carlos Ortega Millán, Fernando Alvarez Guisasola, Juan Carlos Ferrer García, Patxi Ezkurra Loiola, Fernando Escobar Jiménez, José Antonio Fornos Pérez, Mercedes Galindo Rubio, Itxaso Rica Echevarría, Edelmiro Menéndez Torre
No abstract text is available yet for this article.
March 1, 2018: Endocrinología, Diabetes y Nutrición
Georgios Kellaris, Kamal Khan, Shahid M Baig, I-Chun Tsai, Francisca Millan Zamora, Paul Ruggieri, Marvin R Natowicz, Nicholas Katsanis
BACKGROUND: Intellectual disability (ID) is a common condition with a population prevalence frequency of 1-3% and an enrichment for males, driven in part by the contribution of mutant alleles on the X-chromosome. Among the more than 500 genes associated with ID, DDX3X represents an outlier in sex specificity. Nearly all reported pathogenic variants of DDX3X are de novo, affect mostly females, and appear to be loss of function variants, consistent with the hypothesis that haploinsufficiency at this locus on the X-chromosome is likely to be lethal in males...
March 1, 2018: Human Genomics
R Correa, J Gómez-Millán, M Lobato, A Fernández, R Ordoñez, C Castro, Y Lupiañez, J A Medina
Sarcomas are an infrequent and heterogeneous group of neoplasia. Surgery with or without associated radiotherapy (RT) is the basic treatment for this type of tumour. To increase the therapeutic ratio (the index between cytotoxic effects in tumours and normal tissue complications with a certain dose of radiation), new advances are being investigated to increase local and distant control and to decrease the morbidity of the treatment. The aim of this review was to analyse the different strategies, based on technology and biology, which are being investigated to increase the therapeutic ratio of this disease...
February 23, 2018: Clinical & Translational Oncology
Josefina Atrisco-Morales, Verónica I Martínez-Santos, Adolfo Román-Román, Judit Alarcón-Millán, José De Sampedro-Reyes, Iván Cruz-Del Carmen, Dinorah N Martínez-Carrillo, Gloria Fernández-Tilapa
PURPOSE: Virulent genotypes of Helicobacter pylori vacA s1m1/cagA+ /babA2+ have been associated with severe gastric diseases. VacA, CagA and BabA are polymorphic proteins, and their association with the disease is allele-dependent. The aims of this work were: (i) to determine the prevalence of H. pylori by type of chronic gastritis; (ii) to describe the frequency of cagA, babA2 and vacA genotypes in strains from patients with different types of chronic gastritis; (iii) to characterize the variable region of cagA alleles...
January 10, 2018: Journal of Medical Microbiology
Dele Raheem, Conrado Carrascosa, Oluwatoyin Bolanle Oluwole, Maaike Nieuwland, Ariana Saraiva, Rafael Millán, António Raposo
The traditional consumption of edible insects is common in one third of the world's population, mostly in Latin America, Africa and Asia. There are over one thousand identified species of insects eaten in some stage of their life cycle; and they play important roles in ensuring food security. The most common way to collect insects are from the wild, which is seasonal with limited availability and has an increasing demand resulting in a disruption to the ecosystem. There is a growing interest shown in rearing insects for commercial purposes, and an industrial scale production will be required to ensure steady supplies...
February 15, 2018: Critical Reviews in Food Science and Nutrition
Anh Hoang, Susan M Goodman, Iris Y Navarro-Millán, Lisa A Mandl, Mark P Figgie, Mathias P Bostrom, Douglas E Padgett, Peter K Sculco, Alexander S McLawhorn, Jasvinder A Singh
Unfortunately, after publication of this article [1], it was noticed that Table 2 was not correctly formatted during the production process. The corrected Table 2 can be seen below and the original article has been updated to match.
February 14, 2018: Arthritis Research & Therapy
Isidoro Cobo, Paola Martinelli, Marta Flández, Latifa Bakiri, Mingfeng Zhang, Enrique Carrillo-de-Santa-Pau, Jinping Jia, Víctor J Sánchez-Arévalo Lobo, Diego Megías, Irene Felipe, Natalia Del Pozo, Irene Millán, Liv Thommesen, Torunn Bruland, Sara H Olson, Jill Smith, Kristina Schoonjans, William R Bamlet, Gloria M Petersen, Núria Malats, Laufey T Amundadottir, Erwin F Wagner, Francisco X Real
Chronic inflammation increases the risk of developing one of several types of cancer. Inflammatory responses are currently thought to be controlled by mechanisms that rely on transcriptional networks that are distinct from those involved in cell differentiation. The orphan nuclear receptor NR5A2 participates in a wide variety of processes, including cholesterol and glucose metabolism in the liver, resolution of endoplasmic reticulum stress, intestinal glucocorticoid production, pancreatic development and acinar differentiation...
February 14, 2018: Nature
Alessandra Altamirano, Andreas Naschberger, Barbara G Furnrohr, Radka Saldova, Weston B Struwe, Patrick M Jennings, Silvia Millán Martín, Suzana Malic, Immanuel Plangger, Stefan Lechner, Reina Pisano, Nicole Peretti, Bernd Linke, Mario M Aguiar, Friedrich Fresser, Andreas Ritsch, Tihana Lenac Rovis, Christina Goode, Pauline M Rudd, Klaus Scheffzek, Bernhard Rupp, Hans Dieplinger
Afamin is an 87 kDa glycoprotein with five predicted N-glycosylation sites. Afamin's glycan abundance contributes to conformational and chemical inhomogeneity presenting great challenges for molecular structure determination. For the purpose of studying the structure of afamin, various forms of recombinantly expressed human afamin (rhAFM) with different glycosylation patterns were thus created. Wild-type rhAFM and various hypo-glycosylated forms were expressed in CHO, CHO-Lec1 and HEK293T cells. Fully non-glycosylated rhAFM was obtained by transfection of point-mutated cDNA to delete all N-glycosylation sites of afamin...
February 14, 2018: Journal of Proteome Research
Francesca Gattino, Lorella Maniscalco, Selina Iussich, Ilaria Biasato, Marina Martano, Emanuela Morello, Cecilia Gola, Yolanda Millán Ruiz, Nobuo Saeki, Paolo Buracco, Juana Martín de Las Mulas, Raffaella De Maria
Canine mammary tumours (CMTs) are one of the most common malignancies in bitches. Platelet-derived growth factor receptor (PDGFR) α and β, vascular endothelial growth factor receptor-2 (VEGFR-2) and CD117 are tyrosine kinase receptors involved in several tumours and represent suitable targets for specific therapy with toceranib phosphate. The purpose of this study was to evaluate the expression of these receptors in the pathogenesis and progression of CMTs. PDGFRα, PDGFRβ, VEGFR-2 and CD117 were expressed in 46/83 (55...
February 9, 2018: Veterinary Record
Margherita Maranesi, Linda Petrucci, Leonardo Leonardi, Federica Piro, Pilar García Rebollar, Pilar Millán, Paolo Cocci, Cecilia Vullo, Francesco Parillo, Arlindo Moura, Gabriela Gonzalez Mariscal, Cristiano Boiti, Massimo Zerani
To investigate the ovulatory mechanisms triggered by raw semen in rabbits, we examined the expression of nerve growth factor (NGF)-a supposed ovulation-inducing factor (OIF)-and cognate receptors in anterior pituitary, ovary, and cervix as well as plasma NGF and LH concentrations. Six does/group were: sham-inseminated with sterile saline (PBS), naturally mated (NM), inseminated with raw semen alone (RS) or after lumbar anesthesia (ARS) or treatment with COX inhibitors (CIRS). Immunohistochemistry revealed positive signals for NGF and receptors in all tissues...
February 9, 2018: Biology of Reproduction
Libertad Adaya, Modesto Millán, Carlos Peña, Dieter Jendrossek, Guadalupe Espín, Raunel Tinoco-Valencia, Josefina Guzmán, Daniel Pfeiffer, Daniel Segura
A novel poly-3-hydroxybutyrate depolymerase was identified in Azotobacter vinelandii. This enzyme, now designated PhbZ1, is associated to the poly-3-hydroxybutyrate (PHB) granules and when expressed in Escherichia coli, it showed in vitro PHB depolymerizing activity on native or artificial PHB granules, but not on crystalline PHB. Native PHB (nPHB) granules isolated from a PhbZ1 mutant had a diminished endogenous in vitro hydrolysis of the polyester, when compared to the granules of the wild-type strain. This in vitro degradation was also tested in the presence of free coenzyme A...
February 12, 2018: Applied Microbiology and Biotechnology
Maite Calucho, Sara Bernal, Laura Alías, Francesca March, Adoración Venceslá, Francisco J Rodríguez-Álvarez, Elena Aller, Raquel M Fernández, Salud Borrego, José M Millán, Concepción Hernández-Chico, Ivon Cuscó, Pablo Fuentes-Prior, Eduardo F Tizzano
Spinal muscular atrophy (SMA) is a neuromuscular disorder caused by loss or mutations in SMN1. According to age of onset, achieved motor abilities, and life span, SMA patients are classified into type I (never sit), II (never walk unaided) or III (achieve independent walking abilities). SMN2, the highly homologous copy of SMN1, is considered the most important phenotypic modifier of the disease. Determination of SMN2 copy number is essential to establish careful genotype-phenotype correlations, predict disease evolution, and to stratify patients for clinical trials...
January 11, 2018: Neuromuscular Disorders: NMD
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