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https://www.readbyqxmd.com/read/28626479/the-allele-frequency-of-cyp2c9-and-vkorc1-in-the-southern-khorasan-population
#1
Fariba Emadian Razavi, Asghar Zarban, Fatemeh Hajipoor, Mohsen Naseri
The genetic factors are determinants in required dosage changes of warfarin among which are polymorphisms of CYP2C9 and VKORC1 genes. The present study aimed to determine the allele and genotype frequency of CYP2C9 and VKORC1 genes in Birjand population. This study was conducted on 120 individuals who referred to Imam Reza and Vali-Asr hospitals for PT/INR test. After extracting the genomic DNA, the considered sequences were amplified by PCR, and restriction fragment length polymorphism analysis was done by AvaII and KpnI enzymes to determine allele polymorphisms...
June 2017: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28624217/efficacy-of-postnatal-in%C3%A2-vivo-nonsense-suppression-therapy-in-a-pax6-mouse-model-of-aniridia
#2
Xia Wang, Kevin Gregory-Evans, Kishor M Wasan, Olena Sivak, Xianghong Shan, Cheryl Y Gregory-Evans
Nonsense mutations leading to premature stop codons are common occurring in approximately 12% of all human genetic diseases. Thus, pharmacological nonsense mutation suppression strategies would be beneficial to a large number of patients if the drugs could be targeted to the affected tissues at the appropriate time. Here, we used nonsense suppression to manipulate Pax6 dosage at different developmental times in the eye of the small eye (Pax6(Sey/+); G194X) mouse model of aniridia. Efficacy was assessed by functional assays for visual capacity, including electroretinography and optokinetic tracking (OKT), in addition to histological and biochemical studies...
June 16, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28622617/determination-and-pharmacokinetics-of-engeletin-in-rat-plasma-by-ultra-high-performance-liquid-chromatography-with-tandem-mass-spectrometry
#3
Weijian Ye, Ruijie Chen, Wei Sun, Chengke Huang, Xixi Lin, Yaoyao Dong, Congcong Wen, Xianqin Wang
Engeletin, a bioactive flavonoid, has attracted much attention recently by virtue of its multiple biological (anti-diabetic and anti-inflammatory) activities. Despite signifying many therapeutic applications researches indicating quantification or pharmacokinetics of engeletin in biological matrix are still lacking. Here, a simple, sensitive, accurate and reliable ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) approach for the quantification of engeletin in rat plasma was developed and fully validated for the first time...
June 10, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28622067/opioid-involved-overdose-among-male-afghanistan-iraq-era-u-s-military-veterans-a-multidimensional-perspective
#4
Alex S Bennett, Luther Elliott, Andrew Golub, Brett Wolfson-Stofko, Honoria Guarino
BACKGROUND: Mirroring nationwide trends in a broad range of U.S. populations, an alarming number of Afghanistan/Iraq-era U.S. Military veterans have experienced opioid-related overdoses. A growing body of research has examined the proximal behaviors that can precipitate an overdose; considerably less is known about more distal physiological, psychosocial and structural influences on these risk behaviors. OBJECTIVES: This study adopts a multidimensional approach to better understand opioid-related overdose among U...
June 16, 2017: Substance Use & Misuse
https://www.readbyqxmd.com/read/28622024/comparison-of-radiation-exposure-from-fixed-table-fluoroscopy-to-a-portable-c-arm-during-ureteroscopy
#5
Fernando Cabrera, Richard Shin, Kyle Waisanen, Giao Nguyen, Charles D Scales, Michael N Ferrandino, Glenn M Preminger, Terry T Yoshizumi, Michael Eric Lipkin
INTRODUCTION: Fluoroscopy is widely used during endourologic procedures and is associated with treatment required radiation exposure. Our objective was to estimate patient radiation exposure during fluoroscopy with either a fixed table or portable C-arm using a validated anthropomorphic adult male model. MATERIALS AND METHODS: A validated male anthropomorphic model was placed supine on both a cystoscopy operating room table and a fixed fluoroscopy table unit. Metal oxide semiconductor field effect transistor dosimeters were placed at 20 organ-specific locations in the model to measure organ specific dosages...
June 16, 2017: Journal of Endourology
https://www.readbyqxmd.com/read/28621385/electroanalytical-determination-of-escitalopram-oxalate-using-nickel-nanoparticles-modified-carbon-paste-sensor
#6
Kamal Attia, A Mona Mohamed, M Amany Fekry
A sensitive voltammetric method was described for the determination of escitalopram oxalate based on electrocatalytic oxidation at nickel nanoparticles modified chloranil carbon paste sensor in Britton-Robinson buffer (pH range from 2 to 10). The modified electrode was characterized by scanning electron microscopy, electrochemical impedance and cyclic voltammetry. The investigation of electrochemical behavior of escitalopram oxalate was performed using cyclic voltammetry and differential pulse voltammetry. The anodic peak current showed a linear range from 1...
June 2017: Acta Chimica Slovenica
https://www.readbyqxmd.com/read/28611661/discovery-and-development-of-calcium-channel-blockers
#7
REVIEW
Théophile Godfraind
In the mid 1960s, experimental work on molecules under screening as coronary dilators allowed the discovery of the mechanism of calcium entry blockade by drugs later named calcium channel blockers. This paper summarizes scientific research on these small molecules interacting directly with L-type voltage-operated calcium channels. It also reports on experimental approaches translated into understanding of their therapeutic actions. The importance of calcium in muscle contraction was discovered by Sidney Ringer who reported this fact in 1883...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28603124/validation-and-application-of-high-performance-liquid-chromatographic-method-for-the-estimation-of-metoclopramide-hydrochloride-in-plasma
#8
Ahmad Khan, Jallat Khan, Muhammad Irfan, Syed Baqir Shyum Naqvi, Gul Majid Khan, Muhammad Harris Shoaib, Rabia Ismail Yousaf, Amjad Khan
The objective of this study was validation of a reverse phase HPLC method for the estimation of metoclopramide HCl in plasma already validated for determination of metoclopramide HCl in tablets dosage form. A reverse chromatographic method was used for estimation of metoclopramide HCl with the mobile phase of acetonitrile, 20mM potassium dihydrogen phosphate buffer solution (pH 3.0 adjusted with orthophosphoric acid) in the ratio of 40: 60. The column used was Waters C18 3.9×300mm µBondapak (RP). The flow rate of the mobile phase was 2ml/ minute...
January 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28603108/a-validated-stability-indicating-rp-hplc-method-for-estimation-of-armodafinil-in-pharmaceutical-dosage-forms-and-characterization-of-its-base-hydrolytic-product
#9
Kambham Venkateswarlu, Ardhgeri Rangareddy, Kanaka Narasimhaiah, Hemraj Sharma, Naga Mallikarjuna Raja Bandi
The main objective of present study was to develop a RP-HPLC method for estimation of Armodafinil in pharmaceutical dosage forms and characterization of its base hydrolytic product. The method was developed for Armodafinil estimation and base hydrolytic products were characterized. The separation was carried out on C18 column by using mobile phase as mixture of water and methanol (45:55%v/v). Eluents were detected at 220nm at 1ml/min. Stress studies were performed with milder conditions followed by stronger conditions so as to get sufficient degradation around 20%...
January 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28599392/the-laboratory-control-of-anticoagulant-thromboprophylaxis-during-the-early-postpartum-period-after-cesarean-delivery
#10
Ekaterina M Koltsova, Anna N Balandina, Konstantin I Grischuk, Margarita A Shpilyuk, Elena A Seregina, Natalia M Dashkevich, Alexander V Poletaev, Alexey V Pyregov, Gennady T Sukhih, Ilya I Serebriyskiy, Fazly I Ataullakhanov
INTRODUCTION: The incidence of venous thromboembolism (VTE) after cesarean section is up to 0.6%, and the widespread use of cesarean section draws attention to this group. The dosage and duration of low-molecular-weight heparin (LMWH) prophylaxis after delivery is estimated by anamnestic risk-scales; however, the predictive potency for an individual patient's risk can be low. Laboratory hemostasis assays are expected to solve this problem. The aim of this study was to estimate the potency of tests to reflect the coagulation state of patients receiving LMWH in the early postpartum period...
May 25, 2017: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/28599149/an-optimized-lentiviral-vector-system-for-conditional-rnai-and-efficient-cloning-of-microrna-embedded-short-hairpin-rna-libraries
#11
Felix F Adams, Dirk Heckl, Thomas Hoffmann, Steven R Talbot, Arnold Kloos, Felicitas Thol, Michael Heuser, Johannes Zuber, Axel Schambach, Adrian Schwarzer
RNA interference (RNAi) and CRISPR-Cas9-based screening systems have emerged as powerful and complementary tools to unravel genetic dependencies through systematic gain- and loss-of-function studies. In recent years, a series of technical advances helped to enhance the performance of virally delivered RNAi. For instance, the incorporation of short hairpin RNAs (shRNAs) into endogenous microRNA contexts (shRNAmiRs) allows the use of Tet-regulated promoters for synchronous onset of gene knockdown and precise interrogation of gene dosage effects...
September 2017: Biomaterials
https://www.readbyqxmd.com/read/28599099/clinical-and-molecular-genetic-characterization-of-two-siblings-with-trisomy-2p24-3-pter-and-monosomy-5p14-3-pter
#12
Daisuke Fukushi, Kenji Kurosawa, Yasuyo Suzuki, Kaoru Suzuki, Kenichiro Yamada, Seiji Watanabe, Kenji Yokochi, Nobuaki Wakamatsu
Partial trisomy 2p syndrome is occasionally associated with neural tube defects (NTDs), such as anencephaly, encephalocele, and spina bifida, in addition to common features of intellectual disability, developmental delay, and characteristic facial appearance. The 2p24 region has been reported to be associated with NTDs. Here, we report the cases of 2 siblings with trisomy 2p24.3-pter and monosomy 5p14.3-pter caused by the paternal translocation t(2;5)(p24.3;p14.3). Of the two siblings, the elder sister had spina bifida...
June 9, 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/28592174/at-light-speed-advances-in-optogenetic-systems-for-regulating-cell-signaling-and-behavior
#13
Nicole A Repina, Alyssa Rosenbloom, Abhirup Mukherjee, David V Schaffer, Ravi S Kane
Cells are bombarded by extrinsic signals that dynamically change in time and space. Such dynamic variations can exert profound effects on behaviors, including cellular signaling, organismal development, stem cell differentiation, normal tissue function, and disease processes such as cancer. Although classical genetic tools are well suited to introduce binary perturbations, new approaches have been necessary to investigate how dynamic signal variation may regulate cell behavior. This fundamental question is increasingly being addressed with optogenetics, a field focused on engineering and harnessing light-sensitive proteins to interface with cellular signaling pathways...
June 7, 2017: Annual Review of Chemical and Biomolecular Engineering
https://www.readbyqxmd.com/read/28590371/cocaine-abuse-traumatic-brain-injury-and-preexisting-brain-lesions-as-risk-factors-for-bupropion-associated-psychosis
#14
Rajdip Barman, Sanjeev Kumar, Bhuvaneshwar Pagadala, Mark B Detweiler
BACKGROUND AND OBJECTIVE: Bupropion is generally considered safe and is widely used both as a monotherapy and as an augmentation agent for the treatment of major depression. Concerns have been raised about bupropion's propensity to precipitate new psychosis and worsen existing psychotic symptoms, although the mechanism is poorly understood. Three cases are reported in which bupropion use was associated with psychosis. The aim of the study was to explore the risk factors and possible mechanisms of psychosis in each case...
June 5, 2017: Journal of Clinical Psychopharmacology
https://www.readbyqxmd.com/read/28583224/anti-parasitic-effect-of-the-diuretic-and-na-atpase-inhibitor-furosemide-in-cutaneous-leishmaniasis
#15
N Arruda-Costa, D Escrivani, E E Almeida-Amaral, J R Meyer-Fernandes, B Rossi-Bergmann
Leishmania amazonensis promastigotes are known to express furosemide (Lasix®)-sensitive P-type membrane Na+-ATPase. In the present study, furosemide activity was studied in intracellular amastigotes and infected BALB/c mice to investigate its efficacy in cutaneous leishmaniasis (CL). Intracellular parasites, but not macrophages, were found to be sensitive to killing by furosemide (IC50 = 87 µ m vs CC50 ≫ 1000 µ m, respectively). Although furosemide did not induce nitric oxide production or intracellular pH changes in infected macrophages, it led to a significant reactive oxygen species (ROS) burst...
June 6, 2017: Parasitology
https://www.readbyqxmd.com/read/28578848/study-on-pharmacokinetics-and-bioequivalence-of-vonoprazan-pyroglutamate-in-rats-by-liquid-chromatography-with-tandem-mass-spectrometry
#16
Yuan Qiao, Jun Zhao, Xuanfeng Yue, Yang Zhang, Ruitao Zhang, Yuan Xu, Xiuling Tang, Xueying Liu, Qingwei Wang
Vonoprazan Fumarate (TAK-438F) is a new and effective drug approved in Japan in 2014 for treatment and prevention of acid-related diseases (ARDs), which exhibits many advantages compared with traditional proton-pump inhibitors (PPIs). However, the clinical applications of TAK0-438F suffers limitation due to the lack of injection dosage form. Efforts to overcome this limitation lead to the systhesis of Vonoprazan pyroglutamate (TAK-438P) for its high water solubility and more potent antisecretory effect. This was the first report to establish and validate a reliable and sensitive LC-MS/MS method for the quantification of TAK-438P in rat plasma and tissues (heart, liver, spleen, liver, kidney, rain, stomach and small intestine)...
August 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28577599/molecular-inversion-probes-equipped-with-discontinuous-rolling-cycle-amplification-for-targeting-nucleotide-variants-determining-smn1-and-smn2-genes-in-diagnosis-of-spinal-muscular-atrophy
#17
Hwang-Shang Kou, Chun-Chi Wang
The novel techniques of molecular inversion probes (MIPs) combined with discontinuous rolling cycle amplification (DRCA) was developed for determination of the multi-nucleotide variants at single base. The different-length MIPs, a padlock-probe based technology, are designed to simultaneously recognize the identical nucleotide variants. After ligation and DRCA, the different-length genetic products representing the certain genotypes could be simply determined by the short-end capillary electrophoresis (CE) method...
July 18, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28577223/correspondence-between-negative-symptoms-and-potential-sources-of-secondary-negative-symptoms-over-time
#18
Aida Farreny, Mark Savill, Stefan Priebe
There has been a debate in the literature about the distinction between primary and secondary negative symptoms of schizophrenia. Our aim was to study the associations between negative symptoms and potential sources of secondary negative symptoms over time. A sample of 275 participants with at least mid-moderate negative symptoms was randomized into body psychotherapy or Pilates class in a previous study. No significant differences were found between groups over time and changes in the symptom domains were modest...
June 2, 2017: European Archives of Psychiatry and Clinical Neuroscience
https://www.readbyqxmd.com/read/28570428/combined-detection-of-nudt15-variants-could-highly-predict-thiopurine-induced-leukopenia-in-chinese-patients-with-inflammatory-bowel-disease-a-multicenter-analysis
#19
Kang Chao, Xueding Wang, Qian Cao, Jiaming Qian, Kaichun Wu, Xia Zhu, Hong Yang, Jie Liang, Lang Lin, Zicheng Huang, Yu Zhang, Yibiao Huang, Yinghao Sun, Xianmin Xue, Min Huang, Pinjin Hu, Ping Lan, Xiang Gao
BACKGROUND: NUDT15 c.415C>T was a novel genetic marker confirmed in our center for thiopurine-induced leukopenia in Chinese inflammatory bowel disease (IBD). For validation, a large cohort study is needed. Meanwhile, the newly discovered NUDT15 coding variants (c.36_37insGGAGTC and c.52 G>A) have not been studied in patients with IBD. We aimed to further confirm the influence of 3 NUDT15 variants (c.415C>T, c.36_37insGGAGTC, and c.52G>A) on thiopurine-induced leukopenia in Chinese patients with IBD...
May 24, 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28569350/cost-effectiveness-of-posaconazole-tablets-versus-fluconazole-as-prophylaxis-for-invasive-fungal-diseases-in-patients-with-graft-versus-host-disease-after-allogeneic-hematopoietic-stem-cell-transplantation
#20
Santiago Grau, Rafael Cámara, Manuel Jurado, Jaime Sanz, Belén Aragón, Irmina Gozalbo
BACKGROUND: The cost-effectiveness of posaconazole oral suspension versus fluconazole capsules for the prophylaxis of invasive fungal diseases (IFDs) in immunosuppressed allogeneic hematopoietic stem cell transplantation (HSCT) recipients has already been proven. Now, a new solid oral tablet formulation for posaconazole has been developed with improved bioavailability, allowing a reduced daily dosage that can be taken independently of food intake. However, the efficacy of this new formulation should be evaluated since it is associated with a higher cost than the posaconazole oral suspension...
May 31, 2017: European Journal of Health Economics: HEPAC: Health Economics in Prevention and Care
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