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https://www.readbyqxmd.com/read/29163425/features-of-crispr-cas-regulation-key-to-highly-efficient-and-temporally-specific-crrna-production
#1
Andjela Rodic, Bojana Blagojevic, Magdalena Djordjevic, Konstantin Severinov, Marko Djordjevic
Bacterial immune systems, such as CRISPR-Cas or restriction-modification (R-M) systems, affect bacterial pathogenicity and antibiotic resistance by modulating horizontal gene flow. A model system for CRISPR-Cas regulation, the Type I-E system from Escherichia coli, is silent under standard laboratory conditions and experimentally observing the dynamics of CRISPR-Cas activation is challenging. Two characteristic features of CRISPR-Cas regulation in E. coli are cooperative transcription repression of cas gene and CRISPR array promoters, and fast non-specific degradation of full length CRISPR transcripts (pre-crRNA)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163021/schizophrenia-shows-disrupted-links-between-brain-volume-and-dynamic-functional-connectivity
#2
Anees Abrol, Barnaly Rashid, Srinivas Rachakonda, Eswar Damaraju, Vince D Calhoun
Studies featuring multimodal neuroimaging data fusion for understanding brain function and structure, or disease characterization, leverage the partial information available in each of the modalities to reveal data variations not exhibited through the independent analyses. Similar to other complex syndromes, the characteristic brain abnormalities in schizophrenia may be better understood with the help of the additional information conveyed by leveraging an advanced modeling method involving multiple modalities...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/29162202/epidemiological-information-is-key-when-interpreting-whole-genome-sequence-data-lessons-learned-from-a-large-legionella-pneumophila-outbreak-in-warstein-germany-2013
#3
Markus Petzold, Karola Prior, Jacob Moran-Gilad, Dag Harmsen, Christian Lück
IntroductionWhole genome sequencing (WGS) is increasingly used in Legionnaires' disease (LD) outbreak investigations, owing to its higher resolution than sequence-based typing, the gold standard typing method for Legionella pneumophila, in the analysis of endemic strains. Recently, a gene-by-gene typing approach based on 1,521 core genes called core genome multilocus sequence typing (cgMLST) was described that enables a robust and standardised typing of L. pneumophila. Methods: We applied this cgMLST scheme to isolates obtained during the largest outbreak of LD reported so far in Germany...
November 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/29158863/south-south-cooperation-as-a-mechanism-to-strengthen-public-health-services-in-africa-experiences-challenges-and-a-call-for-concerted-action
#4
Olushayo Olu, Amos Petu, Martin Ovberedjo, Diane Muhongerwa
Implementation of new models of development cooperation have been on the increase lately. Coupled with this are calls for use of horizontal development cooperation mechanisms such as South-South Cooperation (SSC) as a way to enhance aid effectiveness in the health sector of developing countries. In this case series, we review recent experiences in the application of SSC initiatives to two public health situations in Africa to demonstrate the veracity of this new paradigm. Our review highlight the immense benefits associated with the use of SSC for health and provide evidence for increasing use of horizontal development coordination mechanisms to strengthen public health services delivery and socioeconomic development among African countries...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/29153400/deciphering-pathogenicity-of-fusarium-oxysporum-from-a-phylogenomics-perspective
#5
Yong Zhang, Li-Jun Ma
Fusarium oxysporum is a large species complex of both plant and human pathogens that attack a diverse array of species in a host-specific manner. Comparative genomic studies have revealed that the host-specific pathogenicity of the F. oxysporum species complex (FOSC) was determined by distinct sets of supernumerary (SP) chromosomes. In contrast to common vertical transfer, where genetic materials are transmitted via cell division, SP chromosomes can be transmitted horizontally between phylogenetic lineages, explaining the polyphyletic nature of the host-specific pathogenicity of the FOSC...
2017: Advances in Genetics
https://www.readbyqxmd.com/read/29149188/orienting-the-causal-relationship-between-imprecisely-measured-traits-using-gwas-summary-data
#6
Gibran Hemani, Kate Tilling, George Davey Smith
Inference about the causal structure that induces correlations between two traits can be achieved by combining genetic associations with a mediation-based approach, as is done in the causal inference test (CIT). However, we show that measurement error in the phenotypes can lead to the CIT inferring the wrong causal direction, and that increasing sample sizes has the adverse effect of increasing confidence in the wrong answer. This problem is likely to be general to other mediation-based approaches. Here we introduce an extension to Mendelian randomisation, a method that uses genetic associations in an instrumentation framework, that enables inference of the causal direction between traits, with some advantages...
November 17, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29149178/drivers-of-genetic-diversity-in-secondary-metabolic-gene-clusters-within-a-fungal-species
#7
Abigail L Lind, Jennifer H Wisecaver, Catarina Lameiras, Philipp Wiemann, Jonathan M Palmer, Nancy P Keller, Fernando Rodrigues, Gustavo H Goldman, Antonis Rokas
Filamentous fungi produce a diverse array of secondary metabolites (SMs) critical for defense, virulence, and communication. The metabolic pathways that produce SMs are found in contiguous gene clusters in fungal genomes, an atypical arrangement for metabolic pathways in other eukaryotes. Comparative studies of filamentous fungal species have shown that SM gene clusters are often either highly divergent or uniquely present in one or a handful of species, hampering efforts to determine the genetic basis and evolutionary drivers of SM gene cluster divergence...
November 17, 2017: PLoS Biology
https://www.readbyqxmd.com/read/29147547/multi-dimensional-charge-transport-in-supramolecular-helical-foldamer-assemblies
#8
Alejandro Méndez-Ardoy, Nagula Markandeya, Xuesong Li, Yu-Tang Tsai, Gilles Pecastaings, Thierry Buffeteau, Victor Maurizot, Luca Muccioli, Frédéric Castet, Ivan Huc, Dario M Bassani
Aromatic foldamers are bioinspired architectures whose potential use in materials remains largely unexplored. Here we report our investigation of vertical and horizontal charge transport over long distances in helical oligo-quinolinecarboxamide foldamers organized as single monolayers on Au or SiO2. Conductive atomic force microscopy showed that vertical conductivity is efficient and that it displays a low attenuation with foldamer length (0.06 Å(-1)). In contrast, horizontal charge transport is found to be negligible, demonstrating the strong anisotropy of foldamer monolayers...
October 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/29140903/cleft-related-orthognathic-surgery-based-on-maxillary-vertical-lengthening-of-the-anterior-facial-height
#9
Woo Shik Jeong, Hyung Hwa Jeong, Soon Man Kwon, Kyung S Koh, Jong Woo Choi
BACKGROUND: The conventional approach during orthognathic surgery for cleft-related deformities has largely focused on restoration of maxilla retrusion, using the maxillary advancement as a standard treatment objective. However, we thought the maxillary vertical shortening and deficient incisor show could be the additional key elements of cleft-related deformities. Although slight vertical lengthening can be obtained with only maxillary advancement, it would not be enough to obtain satisfactory aesthetical results in terms of the anterior facial height...
November 11, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29138630/proteomic-analysis-of-coprinopsis-cinerea-under-conditions-of-horizontal-and-perpendicular-gravity
#10
Ji-Su Kim, Young-Sang Kwon, Dong-Won Bae, Youn-Sig Kwak, Yong-Bum Kwack
Coprinopsis cinerea was employed to investigate the fungal response to gravity. Mycelium growth revealed a consistent growth pattern, irrespective of the direction of gravity (i.e., horizontal vs. perpendicular). However, the fruiting body grew in the direction opposite to that of gravity once the primordia had formed. For the proteomic analysis, only curved-stem samples were used. Fifty-one proteins were identified and classified into 13 groups according to function. The major functional groups were hydrolases and transferases (16%), signal transduction (15%), oxidoreductases and isomerases (11%), carbohydrate metabolism (9%), and transport (5%)...
September 2017: Mycobiology
https://www.readbyqxmd.com/read/29135257/field-theoretic-simulations-of-the-distribution-of-nanorods-in-diblock-copolymer-thin-films
#11
Huikuan Chao, Benjamin J Lindsay, Robert A Riggleman
Using block copolymer microphases to guide the self-assembly of nanorods in thin films can give rise to polymeric materials with unique optical, thermal and mechanical properties beyond those found in neat block copolymers. Often the design and manufacture of the materials require exquisite control of the nanorod distribution, which is experimentally challenging due to the large parameter space spanned by this class of materials. Simulation approaches, on the other hand, can access the thermodynamics that contribute to the nanorod distribution and hence offer valuable aide upon the design and manufacture of the materials...
November 14, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29134153/hgtsim-a-simulator-for-horizontal-gene-transfer-hgt-in-microbial-communities
#12
Weizhi Song, Kerrin Steensen, Torsten Thomas
The development and application of metagenomic approaches have provided an opportunity to study and define horizontal gene transfer (HGT) on the level of microbial communities. However, no current metagenomic data simulation tools offers the option to introduce defined HGT within a microbial community. Here, we present HgtSIM, a pipeline to simulate HGT event among microbial community members with user-defined mutation levels. It was developed for testing and benchmarking pipelines for recovering HGTs from complex microbial datasets...
2017: PeerJ
https://www.readbyqxmd.com/read/29133973/duane-retraction-syndrome-causes-effects-and-management-strategies
#13
REVIEW
Ramesh Kekunnaya, Mithila Negalur
Duane retraction syndrome (DRS) is a congenital eye movement anomaly characterized by variable horizontal duction deficits, with narrowing of the palpebral fissure and globe retraction on attempted adduction, occasionally accompanied by upshoot or down-shoot. The etiopathogenesis of this condition can be explained by a spectrum of mechanical, innervational, neurologic and genetic abnormalities occurring independently or which influence each other giving rise to patterns of clinical presentations along with a complex set of ocular and systemic anomalies...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/29130377/negotiating-horizontality-in-medical-south-south-cooperation-the-cuban-mission-in-rio-de-janeiro-s-urban-peripheries
#14
Maria Lidola, Fabiano Tonaco Borges
For more than 50 years, Cuba has been one of the most important players in the field of international medical cooperation in the Global South. Between 2013 and 2016, Cuba maintained one of its largest cooperations with Brazil: nearly 11,400 Cuban physicians were sent to work within the framework of the Brazilian health programme 'More-Doctors-for-Brazil', which was implemented to improve Brazil's precarious public health sector. This paper inquires into the manifold challenges of horizontal connectivity in this medical South-South cooperation...
November 13, 2017: Global Public Health
https://www.readbyqxmd.com/read/29122122/scaphoid-screw-placement-under-minimal-radiation-exposure
#15
Gloria M Hohenberger, Uldis Berzins, Bore Bakota, Patrick Holweg, Bernhard Clement, Stephan Grechenig
BACKGROUND: Percutaneous scaphoid fixation through either a volar or dorsal approach has the advantage of minor soft tissue damage compared with ORIF, and faster fracture union compared with conservative treatment. However, this technique demands highly intraoperative reliance on X-ray control, including increased radiation exposure and all associated side effects. PURPOSE: To test the possibility and efficacy of volar percutaneous scaphoid screw placement under minimalradiation exposure...
November 2017: Injury
https://www.readbyqxmd.com/read/29121424/numerical-time-domain-modelling-of-hoof-ground-interaction-during-the-stance-phase
#16
R Behnke
BACKGROUND: Hoof-ground interaction impacts on the health and performance characteristics of horses. Due to complex interactions between hoof and ground during the stance phase, previous experimentally dominated studies concentrated on subproblems of the phenomena observed. A multidisciplinary methodology with mathematical modelling, material testing and in-vivo experimental measurements seems promising. OBJECTIVES: With the help of a mathematical approach, this contribution aims to explain from a biomechanical point of view the phenomena observed during experimental investigations (hoof acceleration, interacting forces) and aims to contribute to an overall experimental-mathematical multidisciplinary approach...
November 9, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/29118163/assessment-of-cwd-prion-shedding-in-deer-saliva-with-occupancy-modeling
#17
Kristen A Davenport, Brittany A Mosher, Brian M Brost, Davin M Henderson, Nathaniel D Denkers, Amy V Nalls, Erin McNulty, Candace K Mathiason, Edward A Hoover
Detection of prions is difficult due to the peculiarity of the pathogen, which is a misfolded form of a normal protein. The specificity and sensitivity of detection methods are imperfect in complex samples, including excreta. Here, we combined optimized prion amplification procedures with a statistical method that accounts for false positive and false negative errors to test deer saliva for chronic wasting disease (CWD) prions. This approach enabled us to discriminate shedding of prions in saliva and detection of prions in saliva -- a distinction crucial to understanding the role of prion shedding in disease transmission and for diagnosis...
November 8, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29112886/uncontrolled-manifold-analysis-of-joint-angle-variability-during-table-tennis-forehand
#18
Yoichi Iino, Shinsuke Yoshioka, Senshi Fukashiro
This study was conducted with the objective of evaluating the variance structure of the trunk and racket arm joint angles in table tennis topspin forehand using the uncontrolled manifold (UCM) approach, regarding racket orientation as the task variable. Nine advanced and eight intermediate male collegiate table tennis players performed the topspin strokes against backspin balls. The trunk, upper limb, and racket were modeled as six rigid-link segments with a total of 16 rotation degrees of freedom. The UCM analysis was conducted using 30 trial datasets per participant to quantify the degree of redundancy exploitation needed to stabilize the vertical and horizontal angles of the racket...
November 4, 2017: Human Movement Science
https://www.readbyqxmd.com/read/29111474/the-middle-temporal-artery-surgical-anatomy-and-exposure-for-cerebral-revascularization
#19
Roberto Rodriguez Rubio, Michael T Lawton, Olivia Kola, Halima Tabani, Sonia Yousef, Ali Tayebi Meybodi, Jan-Karl Burkhardt, Ivan El-Sayed, Arnau Benet
BACKGROUND: The middle temporal artery (MTA) is the proximal medial branch of the superficial temporal artery (STA), supplying the temporalis muscle along with deep temporal arteries. Its use in vascularized flaps for reconstructive and otologic procedures has been described, yet its potential use in neurosurgery is not studied. We report a novel technique for exposing the MTA and evaluated its characteristics for extracranial-intracranial (EC-IC) cerebrovascular bypass. METHODS: After a curvilinear frontotemporal incision in 10 cadaveric specimens, the STA was dissected from distal to proximal...
October 27, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/29100147/modelling-hydrodynamics-of-horizontal-flow-steel-slag-filters-designed-to-upgrade-phosphorus-removal-in-small-wastewater-treatment-plants
#20
Cristian Barca, Nicolas Roche, Stéphane Troesch, Yves Andrès, Florent Chazarenc
Steel slag filters, if well designed and operated, may upgrade phosphorus removal in small wastewater treatment plants such as stabilization ponds and constructed wetlands. The main objective of this study was to develop a systemic modelling approach to describe changes in the hydraulic performances and internal hydrodynamics of steel slag filters under real dynamic operating conditions. The experimental retention time distribution curves (RTD curves) determined from tracer experiments performed at different times during the first year of operation of two field-scale steel slag filters were analyzed through a three stage process...
October 31, 2017: Journal of Environmental Management
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