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Validity concepts performance

Peng Yu, Hannes Hahne, Mathias Wilhelm, Bernhard Kuster
Liquid chromatography coupled online to nano-electrospray ionization (nESI) tandem mass spectrometry is the analytical workhorse in the field of proteome research. Dimethyl sulfoxide (DMSO) was recently shown to improve nESI efficiency by a factor of three to ten thus improving the sensitivity and coverage of proteomic experiments. However, relatively few investigations into which solvent additives promote nESI response have been performed at a proteomic scale. Here, we systematically evaluated the concept by screening about 30 compounds with various physico-chemical properties...
October 20, 2016: Analytical and Bioanalytical Chemistry
Kazunari Sasaki, Daisuke Morioka, Simone Conci, Georgios A Margonis, Yu Sawada, Andrea Ruzzenente, Takafumi Kumamoto, Calogero Iacono, Nikolaos Andreatos, Alfredo Guglielmi, Itaru Endo, Timothy M Pawlik
OBJECTIVE: To apply the principles of the Metro-ticket paradigm to develop a prognostic model for patients undergoing hepatic resection of colorectal liver metastasis (CRLM). BACKGROUND: Whereas the hepatocellular "Metro-ticket" prognostic tool utilizes a continuum of tumor size and number, a similar concept of a CRLM Metro-ticket paradigm has not been investigated. METHODS: Tumor Burden Score (TBS) was defined using distance from the origin on a Cartesian plane incorporating maximum tumor size (x-axis) and number of lesions (y-axis)...
October 19, 2016: Annals of Surgery
Sonia Fieulaine, Rodolphe Alves de Sousa, Laure Maigre, Karim Hamiche, Mickael Alimi, Jean-Michel Bolla, Abbass Taleb, Alexis Denis, Jean-Marie Pagès, Isabelle Artaud, Thierry Meinnel, Carmela Giglione
Peptide deformylase (PDF) is considered an excellent target to develop antibiotics. We have performed an extensive characterization of a new PDF from the pathogen Streptococcus agalactiae, showing properties similar to other known PDFs. S. agalactiae PDF could be used as PDF prototype as it allowed to get complete sets of 3-dimensional, biophysical and kinetic data with virtually any inhibitor compound. Structure-activity relationship analysis with this single reference system allowed us to reveal distinct binding modes for different PDF inhibitors and the key role of a hydrogen bond in potentiating the interaction between ligand and target...
October 20, 2016: Scientific Reports
Ammar M Killu, Niyada Naksuk, Christopher V Desimone, Prakriti Gaba, Scott Suddendorf, Joanne Powers, Dejae Ladewig, Lilach O Lerman, Barry A Borlaug, Samuel J Asirvatham
INTRODUCTION: Epicardial procedures frequently require pericardial manipulation. We aimed to develop a nonsurgical percutaneous pericardial modification tool that may (1) facilitate epicardial-based procedures by enabling adhesiolysis or (2) attenuate the myocardial constraining effect of the pericardium. METHODS: Three novel devices were developed to enable pericardiotomy, all of which can be deployed in over-the-wire fashion following percutaneous epicardial access...
October 19, 2016: Journal of Cardiovascular Electrophysiology
Dominique Van de Velde, Ank Eijkelkamp, Wim Peersman, Patricia De Vriendt
BACKGROUND: Over the past decades, there has been a paradigm shift from a purely biomedical towards a bio-psycho-social (BPS) conception of disability and illness, which has led to a change in contemporary healthcare. However, there seems to be a gap between the rhetoric and reality of working within a BPS model. It is not clear whether healthcare professionals show the necessary skills and competencies to act according to the BPS model. OBJECTIVE: The aim of this study was (1) to develop a scale to monitor the BPS competencies of healthcare professionals, (2) to define its factor-structure, (3) to check internal consistency, (4) test-retest reliability and (5) feasibility...
2016: PloS One
Shuai-Bing He, Man-Man Li, Bai-Xia Zhang, Xiao-Tong Ye, Ran-Feng Du, Yun Wang, Yan-Jiang Qiao
During the past decades, there have been continuous attempts in the prediction of metabolism mediated by cytochrome P450s (CYP450s) 3A4, 2D6, and 2C9. However, it has indeed remained a huge challenge to accurately predict the metabolism of xenobiotics mediated by these enzymes. To address this issue, microsomal metabolic reaction system (MMRS)-a novel concept, which integrates information about site of metabolism (SOM) and enzyme-was introduced. By incorporating the use of multiple feature selection (FS) techniques (ChiSquared (CHI), InfoGain (IG), GainRatio (GR), Relief) and hybrid classification procedures (Kstar, Bayes (BN), K-nearest neighbours (IBK), C4...
October 9, 2016: International Journal of Molecular Sciences
Karin S Coyne, Ahmed M Soliman, Mary Kay Margolis, Christine L Thompson, Kristof Chwalisz
OBJECTIVE: To assess the psychometric performance of the 4-week recall version of the Uterine Fibroid Symptom and Health-related Quality of Life Questionnaire (UFS-QoL), a patient measure of the severity of uterine fibroid (UF) symptoms and their impact on health-related quality of life (HRQL). METHODS: This was a retrospective analysis of Phase 2a data from pre-menopausal women with heavy menstrual bleeding associated with UF. Participants completed the UFS-QoL at Baseline, Treatment Month 3, and Follow-up Month 3 and a daily diary with a Menstrual Bleeding Scale and the UF Daily Symptom Scale throughout the study duration...
October 13, 2016: Current Medical Research and Opinion
Kayode K Ojo, Sriveny Dangoudoubiyam, Shiv K Verma, Suzanne Scheele, Amy E DeRocher, Michelle Yeargan, Ryan Choi, Tess R Smith, Kasey L Rivas, Matthew A Hulverson, Lynn K Barrett, Erkang Fan, Dustin J Maly, Marilyn Parsons, Jitender P Dubey, Daniel K Howe, Wesley C Van Voorhis
Sarcocystis neurona is the most frequent cause of equine protozoal myeloencephalitis, a debilitating neurological disease of horses that can be difficult to treat. We identified SnCDPK1, the S. neurona homologue of calcium-dependent protein kinase 1 (CDPK1), a validated drug target in Toxoplasma gondii. SnCDPK1 shares the glycine "gatekeeper" residue of the well-characterized T. gondii enzyme, which allows the latter to be targeted by bumped kinase inhibitors. This study presents detailed molecular and phenotypic evidence that SnCDPK1 can be targeted for rational drug development...
October 8, 2016: International Journal for Parasitology
Yue Zhang, Xueying Zhang, Jingtong Hu, Jiang Nan, Zhenyi Zheng, Zhizhong Zhang, Youguang Zhang, Nicolas Vernier, Dafine Ravelosona, Weisheng Zhao
Racetrack memory (RM) has sparked enormous interest thanks to its outstanding potential for low-power, high-density and high-speed data storage. However, since it requires bi-directional domain wall (DW) shifting process for outputting data, the mainstream stripe-shaped concept certainly suffers from the data overflow issue. This geometrical restriction leads to increasing complexity of peripheral circuits or programming as well as undesirable reliability issue. In this work, we propose and study ring-shaped RM, which is based on an alternative mechanism, spin orbit torque (SOT) driven chiral DW motions...
October 11, 2016: Scientific Reports
Shibing Zhou, Zhenyuan Xu, Fei Liu
It is crucial to determine the optimal number of clusters for the clustering quality in cluster analysis. From the standpoint of sample geometry, two concepts, i.e., the sample clustering dispersion degree and the sample clustering synthesis degree, are defined, and a new clustering validity index is designed. Moreover, a method for determining the optimal number of clusters based on an agglomerative hierarchical clustering (AHC) algorithm is proposed. The new index and the method can evaluate the clustering results produced by the AHC and determine the optimal number of clusters for multiple types of datasets, such as linear, manifold, annular, and convex structures...
October 5, 2016: IEEE Transactions on Neural Networks and Learning Systems
Mikael L Soucisse, Kerianne Boulva, Lucas Sideris, Pierre Drolet, Michel Morin, Pierre Dubé
BACKGROUND: As surgical training is evolving and operative exposure is decreasing, new, effective, and experiential learning methods are needed to ensure surgical competency and patient safety. Video coaching is an emerging concept in surgery that needs further investigation. DESIGN: In this randomized controlled trial conducted at a single teaching hospital, participating residents were filmed performing a side-to-side intestinal anastomosis on cadaveric dog bowel for baseline assessment...
October 5, 2016: Journal of Surgical Education
Mohammad Mahdi Moein, Aziza El Beqqali, Mohamed Abdel-Rehim
Bioanalysis is an essential part in drug discovery and development. Bioanalysis is related to the analysis of analytes (drugs, metabolites, biomarkers) in biological samples and it involves several steps from sample collection to sample analysis and data reporting. The first step is sample collection from clinical or preclinical studies; then sending the samples to laboratory for analysis. Second step is sample clean-up (sample preparation) and it is very important step in bioanalysis. In order to reach reliable results, a robust and stable sample preparation method should be applied...
September 20, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Lucia K Gonzales, Dale Glaser, Lois Howland, Mary Jo Clark, Susie Hutchins, Karen Macauley, Jacqueline F Close, Noelle Lipkin Leveque, Kim Reina Failla, Raelene Brooks, Jillian Ward
BACKGROUND: A number of studies across different disciplines have investigated students' learning styles. Differences are known to exist between graduate and baccalaureate nursing students. However, few studies have investigated the learning styles of students in graduate entry nursing programs. . OBJECTIVES: Study objective was to describe graduate entry nursing students' learning styles. DESIGN/SETTING/PARTICIPANTS/METHODS: A descriptive design was used for this study...
September 28, 2016: Nurse Education Today
Nab Raj Roshyara, Katrin Horn, Holger Kirsten, Peter Ahnert, Markus Scholz
A variety of modern software packages are available for genotype imputation relying on advanced concepts such as pre-phasing of the target dataset or utilization of admixed reference panels. In this study, we performed a comprehensive evaluation of the accuracy of modern imputation methods on the basis of the publicly available POPRES samples. Good quality genotypes were masked and re-imputed by different imputation frameworks: namely MaCH, IMPUTE2, MaCH-Minimac, SHAPEIT-IMPUTE2 and MaCH-Admix. Results were compared to evaluate the relative merit of pre-phasing and the usage of admixed references...
October 4, 2016: Scientific Reports
Christina Theodore-Oklota, Louise Humphrey, Christof Wiesner, Gabriel Schnetzler, Stacie Hudgens, Alicyn Campbell
BACKGROUND: A subcutaneous (SC) formulation of rituximab (MabThera(®)/Rituxan(®)) has been developed that could reduce administration time and improve patient satisfaction with treatment. The Rituximab Administration Satisfaction Questionnaire (RASQ) was created to assess patients' perceptions and satisfaction with rituximab SC (RASQ-SC) or rituximab intravenous (RASQ-IV). We assessed the content validity and psychometric properties of RASQ in patients with non-Hodgkin lymphoma. METHODS: Face and content validity of RASQ-SC and RASQ-IV were qualitatively assessed using 60-minute combined concept elicitation and cognitive debriefing interviews...
2016: Patient Preference and Adherence
Victor Gomel, Philippe R Koninckx
"Microsurgery" is a set of principles developed to improve fertility surgery outcomes. These principles were developed progressively based on common sense and available evidence, under control of clinical feedback obtained with the use of second-look laparoscopy. Fertility outcome was the end point; significant improvement in fertility rates validated the concept clinically. Postoperative adhesion formation being a major cause of failure in fertility surgery, the concept of microsurgery predominantly addresses prevention of postoperative adhesions...
October 2016: Fertility and Sterility
Kathleen S J Preston, Allen W Gottfried, Pamella H Oliver, Adele Eskeles Gottfried, Danielle E Delany, Sirena M Ibrahim
The construct of positive family relationships (PFR), defined as family members getting along well and supporting each other, was investigated in a long-term prospective study. A newly constructed scale of positive family relationships developed using the nominal response model of item-response theory, was subject to a longitudinal network of relations analysis. The conceptualization for this research was founded on a positive psychology framework. Data derived from the Fullerton Longitudinal Study and spanned 20 years from middle childhood (age 9 years) to early adulthood (age 29 years)...
October 2016: Journal of Family Psychology: JFP
Aliaksei Laureshyn, Tim De Ceunynck, Christoffer Karlsson, Åse Svensson, Stijn Daniels
Most existing traffic conflict indicators do not sufficiently take into account the severity of the injuries resulting from a collision had it occurred. Thus far, most of the indicators that have been developed express the severity of a traffic encounter as their proximity to a collision in terms of time or space. This paper presents the theoretical framework and the first implementation of Extended Delta-V as a measure of traffic conflict severity in site-based observations. It is derived from the concept of Delta-V as it is applied in crash reconstructions, which refers to the change of velocity experienced by a road user during a crash...
September 27, 2016: Accident; Analysis and Prevention
Maxime Huet, Myriam Cubizolles, Arnaud Buhot
The process of agglutination is commonly used for the detection of biomarkers like proteins or viruses. The multiple bindings between micrometer sized particles, either latex beads or red blood cells (RBCs), create aggregates that are easily detectable and give qualitative information about the presence of the biomarkers. In most cases, the detection is made by simple naked-eye observation of agglutinates without any access to the kinetics of agglutination. In this study, we address the development of a real-time time observation of RBCs agglutination...
September 20, 2016: Biosensors & Bioelectronics
Panpan Wu, Kewen Xia, Hengyong Yu
BACKGROUND AND OBJECTIVE: Dimensionality reduction techniques are developed to suppress the negative effects of high dimensional feature space of lung CT images on classification performance in computer aided detection (CAD) systems for pulmonary nodule detection. METHODS: An improved supervised locally linear embedding (SLLE) algorithm is proposed based on the concept of correlation coefficient. The Spearman's rank correlation coefficient is introduced to adjust the distance metric in the SLLE algorithm to ensure that more suitable neighborhood points could be identified, and thus to enhance the discriminating power of embedded data...
November 2016: Computer Methods and Programs in Biomedicine
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