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https://www.readbyqxmd.com/read/28165155/performance-of-anti-hcv-testing-in-dried-blood-spots-and-saliva-according-to-hiv-status
#1
Geane Lopes Flores, Helena Medina Cruz, Vanessa Alves Marques, Cristiane Alves Villela-Nogueira, Denise Vigo Potsch, Silvia Beatriz May, Carlos Eduardo Brandão-Mello, Marcia Maria Amendola Pires, Jose Henrique Pilotto, Priscila Pollo-Flores, Eliane Bordalo Cathalá Esberard, Claudia Ivantes, Lia Laura Lewis-Ximenez, Elisabeth Lampe, Livia Melo Villar
The use of saliva and dried blood spots (DBS) could increase access to HCV diagnosis for high-risk populations, such as HIV-infected individuals, but the performance of these assays has not been well established in this group. This study aims to evaluate HIV status, particularly TCD4(+) cell count and viral load, in the performance of anti-HCV testing using DBS and saliva. A total of 961 individuals classified as HCV + , HIV+ or HIV/HCV + , as well as negative controls, donated serum, DBS and saliva samples for anti-HCV testing using a commercial enzyme immunoassay...
February 6, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28146158/poor-sensitivity-of-rapid-tests-for-the-detection-of-antibodies-to-the-hepatitis-b-virus-implications-for-field-studies
#2
Helena Medina Cruz, Leticia de Paula Scalioni, Vanessa Salete de Paula, Juliana Custódio Miguel, Kycia Maria Rodrigues do Ó, Flavio Augusto Pádua Milagres, Marcelo Santos Cruz, Francisco Inácio Bastos, Priscila Pollo Flores, Erotildes Leal, Ana Rita Coimbra Motta-Castro, Lia Laura Lewis-Ximenez, Elisabeth Lampe, Livia Melo Villar
Rapid tests (RTs) can be used as an alternative method for the conventional diagnosis of hepatitis B virus (HBV). This study aims to evaluate antibodies to HBsAg (anti-HBs) and antibodies to HBeAg (anti-HBe) RTs under different Brazilian settings. The following three groups were included: GI: viral hepatitis outpatient services; GII: low resource areas; and GIII: crack users and beauticians. Imuno-rápido anti-HBsAg™ and Imuno-rápido anti-HBeAg™ RTs were evaluated and showed specificities greater than 95% in all groups...
March 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28108401/constitutive-expression-and-characterization-of-a-surface-srs-ncsrs67-protein-of-neospora-caninum-with-no-orthologue-in-toxoplasma-gondii
#3
Marcos Alexandre Bezerra, Luiz Miguel Pereira, Aline Bononi, Carla Agostino Biella, Luciana Baroni, Leticia Pollo-Oliveira, Ana Patrícia Yatsuda
Neospora caninum is a parasite of the Apicomplexa phylum responsible for abortion and losses of fertility in cattle. As part of its intracellular cycle, the first interaction of the parasite with the target cell is performed with the surface proteins known as the SRS superfamily (Surface Antigen Glycoprotein - Related Sequences). SAG related or SRS proteins have been a target of intense research due to its immunodominant pattern, exhibiting potential as diagnostic and/or vaccine candidates. The aim of this study was the cloning, expression and characterization of the gene NcSRS67 of N...
April 2017: Parasitology International
https://www.readbyqxmd.com/read/28099876/antitumoral-activity-of-sesquiterpene-lactone-diacethylpiptocarphol-in-mice
#4
Fronza Alezandre, Sabel Ricardo, Carrenho Luise Zozula Blind, Pollo Luis, Bratti Letícia de Oliveira Souza, Monteiro Fabíola Branco Filippin, Biavatti Maique Weber, Kreuger Maria Regina Orofino
ETHNOPHARMACOLOGICAL RELEVANCE: Sesquiterpene lactones are organic compounds derived from plants that exhibit anti-inflammatory and antitumor activities being one of the locking mechanisms of action of NF-kB pathway and synthesis of cytokines such as IL-1 and TNF- α. AIM OF THE STUDY: The overall objective of the present study was to evaluate the antitumor activity of the sesquiterpene lactone diacethylpiptocarphol (DPC) from Vernonia scorpioides (Lam.) Pers. in animal models Ehrlich tumors that has shown antitumor activity...
January 15, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27997922/continuous-intraoperative-monitoring-of-temporal-lobe-epilepsy-surgery
#5
Rémi Tyrand, Shahan Momjian, Claudio Pollo, Christopher Lysakowski, Agustina M Lascano, Serge Vulliémoz, Karl Schaller, Colette Boëx
BACKGROUND/AIMS: The monitoring of interictal epileptiform discharge rates (IEDRs) all along anterior temporal lobe resections (ATLRs) has never been reported. Here the effect of ATLR on continuous IEDR monitoring is described. METHODS: IEDRs computed automatically during entire interventions were recorded in 34 patients (38.2%, 13/34 depth; 61.8%, 21/34 scalp electrodes only). Monitorings were invalidated when burst suppression occurred or if initial IEDRs were <5...
2016: Stereotactic and Functional Neurosurgery
https://www.readbyqxmd.com/read/27986944/survival-and-development-of-hermetia-illucens-diptera-stratiomyidae-a-biodegradation-agent-of-organic-waste
#6
Ana Clariza Samayoa, Wei-Ting Chen, Shaw-Yhi Hwang
The black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), was reared on artificial diet (wheat bran and chicken feed) in the laboratory at 28ºC (immature stages) and under a greenhouse set at 28ºC (adults). Data were collected and analyzed based on an age-stage, two-sex life table. The intrinsic rate of increase (r), finite rate of increase (λ), net reproduction rate (R0), and mean generation time (T) were 0.0759 (d(-1)), 1.0759 (d(-1)), 68.225 offspring, and 55.635 d, respectively. The maximum reproductive value of females occurred at 54 d...
December 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/27976470/esi-ms-2-and-anti-inflammatory-studies-of-cyclopropanic-triterpenes-uplc-esi-ms-and-ms-2-search-of-related-metabolites-from-donella-ubanguiensis
#7
Louis P Sandjo, Marcus V P Dos Santos Nascimento, Layzon A L da Silva, Antonio C M Munhoz, Luiz A E Pollo, Maique W Biavatti, Bonaventure T Ngadjui, Till Opatz, Tania S Fröde
INTRODUCTION: Triterpenes are one of the largest secondary metabolites groups spread in the plant kingdom with various skeletons. These metabolites have showed various bioactivities including anti-inflammatory activity. OBJECTIVE: The study aims to explore the mass spectrometry fragmentation of donellanic acids A-C (DA A-C), three compounds identified from Donella ubanguiensis; in addition, the fragmentation behaviour of these metabolites will serve as a fingerprint to search and characterise triterpenes congeners in fruits, bark and wood crude extracts of D...
January 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27966027/combined-thalamic-and-subthalamic-deep-brain-stimulation-for-tremor-dominant-parkinson-s-disease
#8
Markus F Oertel, W Michael M Schüpbach, Joseph-André Ghika, Lennart H Stieglitz, Michael Fiechter, Alain Kaelin-Lang, Andreas Raabe, Claudio Pollo
Deep brain stimulation (DBS) in the thalamic ventral intermediate (Vim) or the subthalamic nucleus (STN) reportedly improves medication-refractory Parkinson's disease (PD) tremor. However, little is known about the potential synergic effects of combined Vim and STN DBS. We describe a 79-year-old man with medication-refractory tremor-dominant PD. Bilateral Vim DBS electrode implantation produced insufficient improvement. Therefore, the patient underwent additional unilateral left-sided STN DBS. Whereas Vim or STN stimulation alone led to partial improvement, persisting tremor resolution occurred after simultaneous stimulation...
February 2017: Acta Neurochirurgica
https://www.readbyqxmd.com/read/27843765/t2-relaxometry-predicts-outcome-of-dbs-in-idiopathic-parkinson-s-disease
#9
Tarja Lönnfors-Weitzel, Thilo Weitzel, Johannes Slotboom, Claus Kiefer, Claudio Pollo, Michael Schüpbach, Markus Oertel, Alain Kaelin, Roland Wiest
INTRODUCTION: Deep brain stimulation (DBS) nowadays is a well-established treatment of motor symptoms in Parkinson's disease. The subthalamic nucleus (STN) is a common target for DBS, because motor improvements have been shown to be superior to best medical therapy, if DBS electrodes have been appropriately positioned. DBS target identification can be assisted by MRI beyond structural imaging by spatially resolved measurement of T2-relaxation times (T2r). AIM: We pose the question, whether T2r of the STN is linked to the severity of the disease and whether outcome of DBS may be correlated to an asymmetric manifestation of the disease...
2016: NeuroImage: Clinical
https://www.readbyqxmd.com/read/27761101/measures-of-kindergarten-spelling-and-their-relations-to-later-spelling-performance
#10
Rebecca Treiman, Brett Kessler, Tatiana Cury Pollo, Brian Byrne, Richard K Olson
Learning the orthographic forms of words is important for both spelling and reading. To determine whether some methods of scoring children's early spellings predict later spelling performance better than do other methods, we analyzed data from 374 U.S. and Australian children who took a 10-word spelling test at the end of kindergarten (mean age 6 years, 2 months) and a standardized spelling test approximately two years later. Surprisingly, scoring methods that took account of phonological plausibility did not outperform methods that were based only on orthographic correctness...
2016: Scientific Studies of Reading
https://www.readbyqxmd.com/read/27631560/intraoperative-acceleration-measurements-to-quantify-improvement-in-tremor-during-deep-brain-stimulation-surgery
#11
Ashesh Shah, Jérôme Coste, Jean-Jacques Lemaire, Ethan Taub, W M Michael Schüpbach, Claudio Pollo, Erik Schkommodau, Raphael Guzman, Simone Hemm-Ode
Deep brain stimulation (DBS) surgery is extensively used in the treatment of movement disorders. Nevertheless, methods to evaluate the clinical response during intraoperative stimulation tests to identify the optimal position for the implantation of the chronic DBS lead remain subjective. In this paper, we describe a new, versatile method for quantitative intraoperative evaluation of improvement in tremor with an acceleration sensor that is mounted on the patient's wrist during surgery. At each anatomical test position, the improvement in tremor compared to the initial tremor is estimated on the basis of extracted outcome measures...
September 8, 2016: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/27601248/first-ex-vivo-validation-of-a-radioguided-surgery-technique-with-%C3%AE-radiation
#12
E Solfaroli Camillocci, M Schiariti, V Bocci, A Carollo, G Chiodi, M Colandrea, F Collamati, M Cremonesi, R Donnarumma, M E Ferrari, P Ferroli, F Ghielmetti, C M Grana, C Mancini Terracciano, M Marafini, S Morganti, M Patanè, G Pedroli, B Pollo, L Recchia, A Russomando, M Toppi, G Traini, R Faccini
PURPOSE: A radio-guided surgery technique with β(-)-emitting radio-tracers was suggested to overcome the effect of the large penetration of γ radiation. The feasibility studies in the case of brain tumors and abdominal neuro-endocrine tumors were based on simulations starting from PET images with several underlying assumptions. This paper reports, as proof-of-principle of this technique, an ex vivo test on a meningioma patient. This test allowed to validate the whole chain, from the evaluation of the SUV of the tumor, to the assumptions on the bio-distribution and the signal detection...
September 2016: Physica Medica: PM
https://www.readbyqxmd.com/read/27582543/tumor-initiating-cell-frequency-is-relevant-for-glioblastoma-aggressiveness
#13
Cristina Richichi, Daniela Osti, Massimiliano Del Bene, Lorenzo Fornasari, Monica Patanè, Bianca Pollo, Francesco DiMeco, Giuliana Pelicci
Glioblastoma (GBM) is maintained by a small subpopulation of tumor-initiating cells (TICs). The arduous assessment of TIC frequencies challenges the prognostic role of TICs in predicting the clinical outcome in GBM patients. We estimated the TIC frequency in human GBM injecting intracerebrally in mice dissociated cells without any passage in culture.All GBMs contained rare TICsand were tumorigenic in vivo but only 54% of them grew in vitro as neurospheres. We demonstrated that neurosphere formation in vitro did not foretell tumorigenic ability in vivo and frequencies calculated in vitro overestimated the TIC content...
November 1, 2016: Oncotarget
https://www.readbyqxmd.com/read/27579735/psychometrican-analysis-and-dimensional-structure-of-the-brazilian-version-of-melasma-quality-of-life-scale-melasqol-bp
#14
Camila Fernandes Pollo Maranzatto, Hélio Amante Miot, Luciane Donida Bartoli Miot, Silmara Meneguin
BACKGROUND: Although asymptomatic, melasma inflicts significant impact on quality of life. MELASQoL is the main instrument used to assess quality of life associated with melasma, it has been validated in several languages, but its latent dimensional structure and psychometric properties haven´t been fully explored. OBJECTIVES: To evaluate psychometric characteristics, information and dimensional structure of the Brazilian version of MELASQoL. METHODS: Survey with patients with facial melasma through socio-demographic questionnaire, DLQI-BRA, MASI and MELASQoL-BP, exploratory and confirmatory factor analysis, internal consistency of MELASQoL and latent dimensions (Cronbach's alpha)...
July 2016: Anais Brasileiros de Dermatologia
https://www.readbyqxmd.com/read/27524249/potential-risks-of-the-residue-from-samarco-s-mine-dam-burst-bento-rodrigues-brazil
#15
Fabiana Roberta Segura, Emilene Arusievicz Nunes, Fernanda Pollo Paniz, Ana Carolina Cavalheiro Paulelli, Gabriela Braga Rodrigues, Gilberto Úbida Leite Braga, Walter Dos Reis Pedreira Filho, Fernando Barbosa, Giselle Cerchiaro, Fábio Ferreira Silva, Bruno Lemos Batista
On November 5th, 2015, Samarco's iron mine dam - called Fundão - spilled 50-60 million m(3) of mud into Gualaxo do Norte, a river that belongs to Rio Doce Basin. Approximately 15 km(2) were flooded along the rivers Gualaxo do Norte, Carmo and Doce, reaching the Atlantic Ocean on November 22nd, 2015. Six days after, our group collected mud, soil and water samples in Bento Rodrigues (Minas Gerais, Brazil), which was the first impacted area. Overall, the results, water samples - potable and surface water from river - presented chemical elements concentration according to Brazilian environmental legislations, except silver concentration in surface water that ranged from 1...
November 2016: Environmental Pollution
https://www.readbyqxmd.com/read/27522316/assessment-in-situ-of-genotoxicity-in-tadpoles-and-adults-of-frog-hypsiboas-cordobae-barrio-1965-inhabiting-aquatic-ecosystems-associated-to-fluorite-mine
#16
Favio E Pollo, Pablo R Grenat, Manuel A Otero, Nancy E Salas, Adolfo L Martino
Non-lethal biological techniques such as blood biomarkers have gained attention due to their value as early signals of anthropic effects of contamination representing significant tools to evaluate ecosystems health. We evaluate and characterize in situ genotoxicity of water samples collected from aquatic ecosystems around a fluorite mine using amphibian frogs Hypsiboas cordobae as bioindicator species complemented with 16 physicochemical parameters. Four stations associated with fluorite mine sampling were sampled: a stream running on granitic rock with natural high fluorite content; two streams both running on metamorphic rock with low fluorite content; and an artificial decantation pond containing sediments produced by fluorite flotation process with high variation in physicochemical parameters...
November 2016: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27472540/ictal-time-irreversible-intracranial-eeg-signals-as-markers-of-the-epileptogenic-zone
#17
Kaspar Schindler, Christian Rummel, Ralph G Andrzejak, Marc Goodfellow, Frédéric Zubler, Eugenio Abela, Roland Wiest, Claudio Pollo, Andreas Steimer, Heidemarie Gast
OBJECTIVE: To show that time-irreversible EEG signals recorded with intracranial electrodes during seizures can serve as markers of the epileptogenic zone. METHODS: We use the recently developed method of mapping time series into directed horizontal graphs (dHVG). Each node of the dHVG represents a time point in the original intracranial EEG (iEEG) signal. Statistically significant differences between the distributions of the nodes' number of input and output connections are used to detect time-irreversible iEEG signals...
September 2016: Clinical Neurophysiology: Official Journal of the International Federation of Clinical Neurophysiology
https://www.readbyqxmd.com/read/27329275/a-case-of-medulloblastoma-in-adult-patient-affected-by-anaplastic-oligoastrocytoma
#18
G Simonetti, Paola Gaviani, E Lamperti, A Innocenti, A Botturi, M Saini, B Pollo, A Silvani
Medulloblastomas and high-grade gliomas (HGG) are two distinct brain tumor, with different peculiarities in terms of age of onset, localizations and prognosis. The coexistence of the two neoplasms in the same adult patient is an extremely rare event. We present the case of a woman treated with radio-chemotherapy for an HGG, who developed a cerebellar medulloblastoma 7 years later. Considering the poor prognosis of these tumors, the lack of knowledge about the mechanisms of onset as well as effective therapies, it is necessary to determine the exact role of irradiation and the presence of any potential molecular genetic abnormalities in the developing of the two tumors...
October 2016: Neurological Sciences
https://www.readbyqxmd.com/read/27313308/draft-genome-sequences-of-kosmotoga-sp-strain-du53-and-kosmotoga-arenicorallina-s304
#19
Stephen M J Pollo, Rhianna Charchuk, Camilla L Nesbø
Here, we announce the draft genome sequences of two thermophilic Thermotogae bacteria: Kosmotoga sp. strain DU53, isolated from a continental oil reservoir, and Kosmotoga arenicorallina, isolated from hydrothermal sediments. The sequences will provide further insight into evolution of the Kosmotogales.
2016: Genome Announcements
https://www.readbyqxmd.com/read/27194148/final-results-of-the-second-prospective-aieop-protocol-for-pediatric-intracranial-ependymoma
#20
Maura Massimino, Rosalba Miceli, Felice Giangaspero, Luna Boschetti, Piergiorgio Modena, Manila Antonelli, Paolo Ferroli, Daniele Bertin, Emilia Pecori, Laura Valentini, Veronica Biassoni, Maria Luisa Garrè, Elisabetta Schiavello, Iacopo Sardi, Armando Cama, Elisabetta Viscardi, Giovanni Scarzello, Silvia Scoccianti, Maurizio Mascarin, Lucia Quaglietta, Giuseppe Cinalli, Barbara Diletto, Lorenzo Genitori, Paola Peretta, Anna Mussano, Annamaria Buccoliero, Giuseppina Calareso, Salvina Barra, Angela Mastronuzzi, Carlo Giussani, Carlo Efisio Marras, Rita Balter, Patrizia Bertolini, Ermanno Giombelli, Milena La Spina, Francesca R Buttarelli, Bianca Pollo, Lorenza Gandola
BACKGROUND: This prospective study stratified patients by surgical resection (complete = NED vs incomplete = ED) and centrally reviewed histology (World Health Organization [WHO] grade II vs III). METHODS: WHO grade II/NED patients received focal radiotherapy (RT) up to 59.4 Gy with 1.8 Gy/day. Grade III/NED received 4 courses of VEC (vincristine, etoposide, cyclophosphamide) after RT. ED patients received 1-4 VEC courses, second-look surgery, and 59.4 Gy followed by an 8-Gy boost in 2 fractions on still measurable residue...
October 2016: Neuro-oncology
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