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minor blood group antibodies

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https://www.readbyqxmd.com/read/29387320/relationship-between-%C3%AE-thalassemia-minor-and-helicobacter-pylori-infection
#1
Mohammad Zamani, Amin Vahedi, Ahmad Tamaddoni, Ali Bijani, Mojgan Bagherzade, Javad Shokri-Shirvani
Background: Until now, no study has been reported investigating the association between β-thalassemia minor and Helicobacter pylori (H. pylori) infection. This study was designed to compare H. pylori infection rate between β-thalassemia minor patients and healthy controls. Methods: A number of 100 β-thalassemia minor patients (50 males, 50 females) and 100 gender-matched healthy controls were prospectively recruited in this study in a period of 3 months. The study population consisted of the people who referred to a health center in Babol, North of Iran, for premarital counseling...
2018: Caspian Journal of Internal Medicine
https://www.readbyqxmd.com/read/29295661/neonatal-hyperbilirubinemia-secondary-to-combined-anti-e-and-anti-c-isoimmunisation-a-literature-review
#2
Deepak Sharma, Nazanin Farahbakhsh
A term male infant was admitted at 48 hours of postnatal life to the neonatal unit for jaundice. The investigation showed total serum bilirubin (TSB) of 17.1 mg/dl, haemoglobin of 11 g/dl, reticulocyte count of 9.5% and peripheral smear was suggestive of macrocytic, normochromic red blood cell (RBC) with target cells and multiple spherocytes with occasional nucleated RBC. The infant's blood group was B positive. Direct antiglobulin test was strongly positive by gel method (3+). Mother's blood group was B positive and indirect antiglobulin test was positive when tested postnatally...
January 2, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29274773/nerve-growth-factor-plays-a-role-in-the-neurotherapeutic-effect-of-a-cd45-pan-hematopoietic-subpopulation-derived-from-human-umbilical-cord-blood-in-a-traumatic-brain-injury-model
#3
Galit Gincberg, Esther Shohami, Victoria Trembovler, Alexander G Alexandrovich, Philip Lazarovici, Uriel Elchalal
BACKGROUND AIMS: Human umbilical cord blood (HUCB) is an important source of stem cells for therapy of hematopoietic disorders and is a potential therapy for various neurological disorders, including traumatic brain injury (TBI). The expression of nerve growth factor (NGF) and its receptors TrkA, p75NTR and α9β1 integrin on an HUCB CD45+ pan-hematopoietic subpopulation was investigated in the context of its neurotherapeutic potential after TBI. METHODS: NGF and its receptors were detected on CD45+ cells by reverse transcriptase polymerase chain reaction, flow cytometry analysis and confocal microscopy...
February 2018: Cytotherapy
https://www.readbyqxmd.com/read/29153948/rh-blood-phenotyping-d-e-e-c-c-microarrays-using-multichannel-surface-plasmon-resonance-imaging
#4
Chinnawut Pipatpanukul, Sasaki Takeya, Akira Baba, Ratthasart Amarit, Armote Somboonkaew, Boonsong Sutapun, Pimpun Kitpoka, Mongkol Kunakorn, Toemsak Srikhirin
The application of Surface Plasmon Resonance Imaging (SPRi) for the detection of transmembrane antigen of the Rhesus (Rh) blood group system is demonstrated. Clinically significant Rh blood group system antigens, including D, C, E, c, and e, can be simultaneously identified via solid phase immobilization assay, which offers significant time savings and assay simplification. Red blood cells (RBCs) flowed through the micro-channel, where a suitable condition for Rh blood group detection was an RBC dilution of 1:10 with a stop-flow condition...
November 2, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28991900/intra-individual-variability-and-circadian-rhythm-of-vascular-endothelial-growth-factors-in-subjects-with-normal-glucose-tolerance-and-type-2-diabetes
#5
Markolf Hanefeld, Katrin Engelmann, Dieter Appelt, Dirk Sandner, Ingo Weigmann, Xenia Ganz, Frank Pistrosch, Carsta Köhler, Antje Gasparic, Andreas L Birkenfeld
Increased levels of systemic vascular endothelial growth factors (VEGFs) in patients with diabetes are associated with increased risk of microvessel disease. On the other hand, low VEGF levels after intravitreal antibody application may be associated with acute cardiovascular complications and treatment failure. Individual levels of systemic VEGF vary in a wide range depending on analytical methods and quality of diabetes control. So far only limited information exists on intraindividual fluctuations over longer periods and circadian rhythms...
2017: PloS One
https://www.readbyqxmd.com/read/28942078/tau-protein-mapt-as-a-possible-biochemical-marker-of-traumatic-brain-injury-in-postmortem-examination
#6
Mieszko Olczak, Justyna Niderla-Bielińska, Magdalena Kwiatkowska, Dorota Samojłowicz, Sylwia Tarka, Teresa Wierzba-Bobrowicz
MAPT is a neuronal protein that plays an important role in axonal stabilization, neuronal development, and neuronal polarity. MAPT release into the CSF and blood has been interpreted as indicative of axonal injury as its elevated levels were observed in olympic boxers even after a mild head trauma suggesting minor CNS injuries. In our study we wanted to check the potential relevance of MAPT examination for forensic purposes. The study was carried out using cases of head injury group and cases of sudden death (cardiopulmonary failure, no injuries of the head - control group) provided by forensic pathologists at the Department of Forensic Medicine, Medical University of Warsaw...
November 2017: Forensic Science International
https://www.readbyqxmd.com/read/28834647/clinical-and-pathological-features-of-anca-associated-vasculitis-in-patients-with-minor-urinary-abnormalities
#7
Jumpei Hasegawa, Junichi Hoshino, Akinari Sekine, Noriko Hayami, Tatsuya Suwabe, Keiichi Sumida, Koki Mise, Toshiharu Ueno, Masayuki Yamanouchi, Ryo Hazue, Naoki Sawa, Kenichi Ohashi, Takeshi Fujii, Kenmei Takaichi, Yoshifumi Ubara
BACKGROUND: Kidney biopsy is a gold standard for diagnosis of anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV), but it is unknown whether vasculitis can be detected from AAV patients with minor urinary abnormalities. METHODS: Ninety ANCA-positive patients undergoing kidney biopsy were evaluated retrospectively after being divided into two groups, which were group A (minor urinary abnormalities with both proteinuria <0.5 g/day and red blood cells ≤5 /high power field) and group B (major urinary abnormalities except group A)...
August 23, 2017: Nephrology
https://www.readbyqxmd.com/read/28708892/cross-recognition-of-a-pit-viper-crotalinae-polyspecific-antivenom-explored-through-high-density-peptide-microarray-epitope-mapping
#8
Mikael Engmark, Bruno Lomonte, José María Gutiérrez, Andreas H Laustsen, Federico De Masi, Mikael R Andersen, Ole Lund
Snakebite antivenom is a 120 years old invention based on polyclonal mixtures of antibodies purified from the blood of hyper-immunized animals. Knowledge on antibody recognition sites (epitopes) on snake venom proteins is limited, but may be used to provide molecular level explanations for antivenom cross-reactivity. In turn, this may help guide antivenom development by elucidating immunological biases in existing antivenoms. In this study, we have identified and characterized linear elements of B-cell epitopes from 870 pit viper venom protein sequences by employing a high-throughput methodology based on custom designed high-density peptide microarrays...
July 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28556615/characteristics-of-germinal-center-like-structures-in-patients-with-sj%C3%A3-gren-s-syndrome
#9
Jing He, Yuebo Jin, Xia Zhang, Yunshan Zhou, Ru Li, Yijun Dai, Xiaolin Sun, Jingzhong Zhao, Jianping Guo, Zhanguo Li
AIM: To analyze the relationship between ectopic germinal centers (GCs) in the salivary glands and the clinical/laboratory characteristics of patients with Sjögren's syndrome (SS). METHODS: Retrospectively, 126 patients with primary SS (pSS) and 16 patients with secondary SS (sSS) were analyzed. Minor salivary gland biopsies were evaluated for the presence of GC-like morphology by hematoxylin and eosin (H&E) and immunohistochemical (IHC) staining for CD21. Clinical and serological data were obtained from medical records...
February 2017: International Journal of Rheumatic Diseases
https://www.readbyqxmd.com/read/28474625/immunogenicity-safety-of-a-single-dose-of-live-attenuated-japanese-encephalitis-vaccine-sa-14-14-2-in-adults
#10
Siraj Ahmed Khan, Sanjeeb Kakati, Prafulla Dutta, Purvita Chowdhury, Jani Borah, Rashmee Topno, Santoshkumar M Jadhav, Pradyumna K Mohapatra, Jagadish Mahanta, Mohan D Gupte
BACKGROUND & OBJECTIVES: Japanese encephalitis (JE) caused by mosquito-borne Flavivirus is one of the leading causes of viral encephalitis in Asia. Control strategies include vector control and human vaccination. Due to lack of immunization programmes in endemic regions, there are still high mortality and morbidity. A live-attenuated SA 14-14-2 JE vaccine (LAJEV) has been licensed and used in Asian countries, including India. We report the assessment of immunogenicity and safety of the vaccine in adults during the first mass adult vaccination campaign carried out in Assam, India...
December 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/28333830/minimal-variation-in-anti-a-and-b-titers-among-healthy-volunteers-over-time-implications-for-the-use-of-out-of-group-blood-components
#11
Ulrik Sprogøe, Mark H Yazer, Mads Hvidkjær Rasmussen, Berit Antonsen, Claus Bistrup, Kristian Assing
BACKGROUND: Using potentially out-of-group blood components, like low titer A plasma and O whole blood, in the resuscitation of trauma patients is becoming increasingly popular. However, very little is known whether the donors' anti-A and/or anti-B titers change over time and whether repeated titer measurements on the same donor are required to ensure that each donation produces a low titer product. METHODS: The anti-A and/or anti-B titers were measured on 56 healthy adult volunteers (47 blood donors; nine blood center personnel) every 3 months for 12 consecutive months using an automated solid phase analyzer...
June 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28285160/ethanol-dispersed-and-antibody-conjugated-polymer-nanofibers-for-the-selective-capture-and-3-dimensional-culture-of-epcam-positive-cells
#12
Junghyo Yoon, Hee-Sook Yoon, Yoojin Shin, Sanghyun Kim, Youngjun Ju, Jungbae Kim, Seok Chung
Electrospun and ethanol-dispersed polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers (NFs) were used as a platform for the selective capture and three-dimensional culture of EpCAM-positive cells in cell culture medium and whole blood. The NFs were treated with streptavidin to facilitate bond formation between the amino groups of streptavidin and the maleic anhydride groups of the NFs. A biotinylated anti-EpCAM monoclonal antibody (mAb) was attached to the streptavidin-conjugated NFs via the selective binding of streptavidin and biotin...
July 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/27824739/effect-of-the-lymphocyte-to-monocyte-ratio-on-the-clinical-outcome-of-chemotherapy-administration-in-advanced-melanoma-patients
#13
Alexey A Leontovich, Roxana S Dronca, Wendy K Nevala, Michael A Thompson, Lisa A Kottschade, Leonid V Ivanov, Svetomir N Markovic
Skin cancer affects more individuals in the USA than any other malignancy and malignant melanoma is particularly deadly because of its metastatic potential. Melanoma has been recognized as one of the most immunogenic malignancies; therefore, understanding the mechanisms of tumor-immune interaction is key for developing more efficient treatments. As the tumor microenvironment shows an immunosuppressive action, immunotherapeutic agents promoting endogenous immune response to cancer have been tested (interleukin-2, anticytotoxic-T-lymphocyte-associated antigen 4, and antiprogrammed cell death protein 1 monoclonal antibodies) as well as combinations of cytotoxic chemotherapy agents and inhibitors of angiogenesis (taxol/carboplatin/avastin)...
February 2017: Melanoma Research
https://www.readbyqxmd.com/read/27812263/clinical-significance-of-rare-maternal-anti-jk-a-antibody
#14
Kshitija Mittal, Tanvi Sood, Naveen Bansal, Ravneet Kaur Bedi, Paramjit Kaur, Gagandeep Kaur
We hereby report a rare case of HDFN because of antibody to Kidd (Jk) blood group system-anti Jk(a). An EDTA sample of a baby along with mother's sample was received in the Department for Direct Antiglobulin Test (DAT) alongwith blood requisition for double volume exchange transfusion. On blood grouping, baby's and mother's blood group was found to be B Rh D positive. DAT with polyspecific anti human globulin (AHG) was positive. Screening of mother's serum for irregular antibodies showed anti-Jk(a) antibody...
December 2016: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/27603310/stabilization-of-transfected-cells-expressing-low-incidence-blood-group-antigens-novel-methods-facilitating-their-use-as-reagent-cells
#15
Cecilia González, Rosa Esteban, Carme Canals, Eduardo Muñiz-Díaz, Núria Nogués
BACKGROUND: The identification of erythrocyte antibodies in the serum of patients rely on panels of human red blood cells (RBCs), which coexpress many antigens and are not easily available for low-incidence blood group phenotypes. These problems have been addressed by generating cell lines expressing unique blood group antigens, which may be used as an alternative to human RBCs. However, the use of cell lines implies several drawbacks, like the requirement of cell culture facilities and the high cost of cryopreservation...
2016: PloS One
https://www.readbyqxmd.com/read/27549676/the-efficacy-of-rituximab-in-high-risk-renal-transplant-recipients
#16
Motoo Araki, Koichiro Wada, Yosuke Mitsui, Risa Kubota, Takashi Yoshioka, Yuichi Ariyoshi, Yasuyuki Kobayashi, Masashi Kitagawa, Katsuyuki Tanabe, Hiroshi Sugiyama, Jun Wada, Masami Watanabe, Toyohiko Watanabe, Katsuyuki Hotta, Yasutomo Nasu
Although graft survival following renal transplantation (RTx) has improved, outcomes following highrisk RTx are variable. Preexisting antibodies, including donor-specific antibodies (DSA), play an important role in graft dysfunction and survival. We have designed a study to investigate the safety and efficacy of anti-CD20 monoclonal antibodies (rituximab) in high-risk RTx recipients. Major eligibility criteria include: 1) major and minor ABO blood group mismatch, 2) positive DSA. Thirty-five patients will receive 200 mg/body of rituximab...
August 2016: Acta Medica Okayama
https://www.readbyqxmd.com/read/27431816/characterization-of-glycoproteins-expressing-the-blood-group-h-type-1-epitope-on-human-induced-pluripotent-stem-hips-cells
#17
Hiromi Nakao, Shogo Matsumoto, Yuko Nagai, Aya Kojima, Hidenao Toyoda, Noritaka Hashii, Daisuke Takakura, Nana Kawasaki, Tomoko Yamaguchi, Kenji Kawabata, Nobuko Kawasaki, Toshisuke Kawasaki
Recently, we established two mouse monoclonal antibodies (R-10G and R-17F). The R-17F antibody (IgG1 subtype) exhibited a strong cytotoxic effect on hiPS/ES cells. The R-17F antigen isolated from a total lipid extract of hiPS (Tic) cells was identified as LNFP I (Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1-4Glc). In the present study, R-17F binding proteins were isolated from hiPS (Tic) cell lysates with an affinity column of R-17F. They gave one major R-17F positive band around 250 kDa, and several minor bands between 150 kDa and 25 kDa...
July 19, 2016: Glycoconjugate Journal
https://www.readbyqxmd.com/read/27318433/alloimmunization-against-platelets-granulocytes-and-erythrocytes-in-multi-transfused-patients-in-iranian-population
#18
MULTICENTER STUDY
Mohammad Reza Younesi, Samira Louni Aligoudarzi, Razieh Bigdeli, Marzieh Lashgari, Hoda Mazaheri, Vahid Asgary
BACKGROUND: This study aims at alloantibody screening, determination of the types of these antibodies in multiple-transfused patients with chronic hematologic diseases. PATIENTS AND METHODS: This descriptive study was performed on 240 patients with chronic hematological diseases referred to public hospitals in Iran. Single blood sample was taken and tested for the presence of antibodies. In case of a positive antibody screening, antibody identification was performed using granulocyte agglutination test (GAT), granulocyte indirect immunofluorescence test (GIIFT), platelet indirect immunofluorescence test (PIIFT), monoclonal antibody-specific immobilization of platelet antigen (MAIPA) and panel cells...
October 2016: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/27142328/pertussis-vaccination-during-pregnancy-in-belgium-follow-up-of-infants-until-1-month-after-the-fourth-infant-pertussis-vaccination-at-15-months-of-age
#19
Kirsten Maertens, Raïssa Nadège Caboré, Kris Huygen, Sandra Vermeiren, Niel Hens, Pierre Van Damme, Elke Leuridan
Vaccination of pregnant women with a pertussis containing vaccine is a recommended strategy in some industrialized countries, to protect young infants from severe disease. One of the effects of the presence of high titers of passively acquired maternal antibodies in young infants is blunting of immune responses to infant vaccination. We present infant immune responses to a fourth pertussis containing vaccine dose at 15 months of age, as a follow-up of previously presented data. In a prospective cohort study, women were either vaccinated with an acellular pertussis vaccine (Boostrix(®)) during pregnancy (vaccine group) or received no vaccine (control group)...
June 30, 2016: Vaccine
https://www.readbyqxmd.com/read/26913424/-study-on-serological-blood-group-conversion-in-bm-empty-phase-of-abo-incompatible-allogeneic-stem-cell-transplantation
#20
Xue-Jiao Cai, Bi-Le Chen, Zuo-Ting Xie, Yin-Cai Ye
OBJECTIVE: To explore the regularity of serological conversion of blood group in BM empty phase of ABO-incompatible allogeneic stem cell transplantation so as to provide the basis for selecting the blood components in blood transfusion. METHODS: Before hematpoietic stem cell transplantation (HSCT), the ABO and RhD blood groups of recipients and donors were identified by salt medium tube method and microcolumn gel method; after transplantation the changes of antigen intensity and antibody titer of ABO blood group in patients were periodically detected...
February 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
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