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minor blood group antibodies

Alexey A Leontovich, Roxana S Dronca, Wendy K Nevala, Michael A Thompson, Lisa A Kottschade, Leonid V Ivanov, Svetomir N Markovic
Skin cancer affects more individuals in the USA than any other malignancy and malignant melanoma is particularly deadly because of its metastatic potential. Melanoma has been recognized as one of the most immunogenic malignancies; therefore, understanding the mechanisms of tumor-immune interaction is key for developing more efficient treatments. As the tumor microenvironment shows an immunosuppressive action, immunotherapeutic agents promoting endogenous immune response to cancer have been tested (interleukin-2, anticytotoxic-T-lymphocyte-associated antigen 4, and antiprogrammed cell death protein 1 monoclonal antibodies) as well as combinations of cytotoxic chemotherapy agents and inhibitors of angiogenesis (taxol/carboplatin/avastin)...
February 2017: Melanoma Research
Kshitija Mittal, Tanvi Sood, Naveen Bansal, Ravneet Kaur Bedi, Paramjit Kaur, Gagandeep Kaur
We hereby report a rare case of HDFN because of antibody to Kidd (Jk) blood group system-anti Jk(a). An EDTA sample of a baby along with mother's sample was received in the Department for Direct Antiglobulin Test (DAT) alongwith blood requisition for double volume exchange transfusion. On blood grouping, baby's and mother's blood group was found to be B Rh D positive. DAT with polyspecific anti human globulin (AHG) was positive. Screening of mother's serum for irregular antibodies showed anti-Jk(a) antibody...
December 2016: Indian Journal of Hematology & Blood Transfusion
Cecilia González, Rosa Esteban, Carme Canals, Eduardo Muñiz-Díaz, Núria Nogués
BACKGROUND: The identification of erythrocyte antibodies in the serum of patients rely on panels of human red blood cells (RBCs), which coexpress many antigens and are not easily available for low-incidence blood group phenotypes. These problems have been addressed by generating cell lines expressing unique blood group antigens, which may be used as an alternative to human RBCs. However, the use of cell lines implies several drawbacks, like the requirement of cell culture facilities and the high cost of cryopreservation...
2016: PloS One
Motoo Araki, Koichiro Wada, Yosuke Mitsui, Risa Kubota, Takashi Yoshioka, Yuichi Ariyoshi, Yasuyuki Kobayashi, Masashi Kitagawa, Katsuyuki Tanabe, Hiroshi Sugiyama, Jun Wada, Masami Watanabe, Toyohiko Watanabe, Katsuyuki Hotta, Yasutomo Nasu
Although graft survival following renal transplantation (RTx) has improved, outcomes following highrisk RTx are variable. Preexisting antibodies, including donor-specific antibodies (DSA), play an important role in graft dysfunction and survival. We have designed a study to investigate the safety and efficacy of anti-CD20 monoclonal antibodies (rituximab) in high-risk RTx recipients. Major eligibility criteria include: 1) major and minor ABO blood group mismatch, 2) positive DSA. Thirty-five patients will receive 200 mg/body of rituximab...
August 2016: Acta Medica Okayama
Hiromi Nakao, Shogo Matsumoto, Yuko Nagai, Aya Kojima, Hidenao Toyoda, Noritaka Hashii, Daisuke Takakura, Nana Kawasaki, Tomoko Yamaguchi, Kenji Kawabata, Nobuko Kawasaki, Toshisuke Kawasaki
Recently, we established two mouse monoclonal antibodies (R-10G and R-17F). The R-17F antibody (IgG1 subtype) exhibited a strong cytotoxic effect on hiPS/ES cells. The R-17F antigen isolated from a total lipid extract of hiPS (Tic) cells was identified as LNFP I (Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1-4Glc). In the present study, R-17F binding proteins were isolated from hiPS (Tic) cell lysates with an affinity column of R-17F. They gave one major R-17F positive band around 250 kDa, and several minor bands between 150 kDa and 25 kDa...
July 19, 2016: Glycoconjugate Journal
Mohammad Reza Younesi, Samira Louni Aligoudarzi, Razieh Bigdeli, Marzieh Lashgari, Hoda Mazaheri, Vahid Asgary
BACKGROUND: This study aims at alloantibody screening, determination of the types of these antibodies in multiple-transfused patients with chronic hematologic diseases. PATIENTS AND METHODS: This descriptive study was performed on 240 patients with chronic hematological diseases referred to public hospitals in Iran. Single blood sample was taken and tested for the presence of antibodies. In case of a positive antibody screening, antibody identification was performed using granulocyte agglutination test (GAT), granulocyte indirect immunofluorescence test (GIIFT), platelet indirect immunofluorescence test (PIIFT), monoclonal antibody-specific immobilization of platelet antigen (MAIPA) and panel cells...
October 2016: Transfusion and Apheresis Science
Kirsten Maertens, Raïssa Nadège Caboré, Kris Huygen, Sandra Vermeiren, Niel Hens, Pierre Van Damme, Elke Leuridan
Vaccination of pregnant women with a pertussis containing vaccine is a recommended strategy in some industrialized countries, to protect young infants from severe disease. One of the effects of the presence of high titers of passively acquired maternal antibodies in young infants is blunting of immune responses to infant vaccination. We present infant immune responses to a fourth pertussis containing vaccine dose at 15 months of age, as a follow-up of previously presented data. In a prospective cohort study, women were either vaccinated with an acellular pertussis vaccine (Boostrix(®)) during pregnancy (vaccine group) or received no vaccine (control group)...
June 30, 2016: Vaccine
Xue-Jiao Cai, Bi-Le Chen, Zuo-Ting Xie, Yin-Cai Ye
OBJECTIVE: To explore the regularity of serological conversion of blood group in BM empty phase of ABO-incompatible allogeneic stem cell transplantation so as to provide the basis for selecting the blood components in blood transfusion. METHODS: Before hematpoietic stem cell transplantation (HSCT), the ABO and RhD blood groups of recipients and donors were identified by salt medium tube method and microcolumn gel method; after transplantation the changes of antigen intensity and antibody titer of ABO blood group in patients were periodically detected...
February 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
Andreas Thomas, Paul Brinkkötter, Wilhelm Schänzer, Mario Thevis
The misuse of insulin for performance enhancement in sport or as toxic agent has frequently been reported in the past. In contrast to synthetic insulin analogues, the administration of recombinant human insulin is hardly recognized by mass spectrometry. The present study was designed to uncover the misuse of recombinant human insulin for doping control purposes as well as for forensic applications. It is hypothesized that an altered metabolite profile of circulating insulin prevails after subcutaneous administration due to exposure of insulin to epidermal proteases...
October 15, 2015: Analytica Chimica Acta
Hooshang Lahooti, Daniele Cultrone, Senarath Edirimanne, John P Walsh, Leigh Delbridge, Patrick Cregan, Bernard Champion, Jack R Wall
BACKGROUND: The eye disorder associated with Graves' disease, called Graves' ophthalmopathy (GO), greatly reduces the quality of life in affected patients. Expression of the calsequestrin (CASQ1) protein in thyroid tissue may be the trigger for the development of eye muscle damage in patients with GO. We determined the prevalence of rs74123279, rs3747673, and rs2275703 single-nucleotide polymorphism (SNPs) in patients with autoimmune thyroid disorders, GO, Graves' hyperthyroidism (GH), or Hashimoto's thyroiditis (HT) and control subjects with no personal or family history of autoimmune thyroid disorders...
2015: Clinical Ophthalmology
Sabita Basu, Supriya Dhar, Deepak Mishra, Mammen Chandy
BACKGROUND: The ABO blood group system is of prime significance in red cell transfusion and organ transplantation. However, ABO compatibility is not critical in allogenic hemopoietic stem cell transplantation (HSCT) and approximately 40-50% of hemopoietic stem cell transplants are ABO incompatible. This incompatibility may be major, minor or bi-directional. Though there are descriptions of transfusion practice and protocols in ABO incompatible HSCT, there are considerable variations and transfusion support in these patients can be very challenging...
July 2015: Asian Journal of Transfusion Science
Tamima Al-Dughaishi, Ikhlass S Al-Rubkhi, Maymoona Al-Duhli, Yusra Al-Harrasi, Vaidyanathan Gowri
OBJECTIVE: This study is aimed to determine the prevalence of alloimmunization due to antibodies to red blood cell (RBC) antigens (other than rhesus [Rh] antigen) and report the maternal, perinatal, and neonatal outcomes. MATERIALS AND METHODS: A retrospective review of medical records of all patients with minor RBCs antibodies alloimmunization who were followed and delivered at Sultan Qaboos University Hospital, Oman from June 2011 to June 2013. Maternal characteristics, antibody type, antibody titer in addition to perinatal and neonatal outcomes were reviewed...
July 2015: Asian Journal of Transfusion Science
Hani Boshra, Thang Truong, Charles Nfon, Timothy R Bowden, Volker Gerdts, Suresh Tikoo, Lorne A Babiuk, Pravesh Kara, Arshad Mather, David B Wallace, Shawn Babiuk
Sheep and goat pox continue to be important livestock diseases that pose a major threat to the livestock industry in many regions in Africa and Asia. Currently, several live attenuated vaccines are available and used in endemic countries to control these diseases. One of these is a partially attenuated strain of lumpy skin disease virus (LSDV), KS-1, which provides cross-protection against both sheep pox and goat pox. However, when used in highly stressed dairy cattle to protect against lumpy skin disease (LSD) the vaccine can cause clinical disease...
November 2015: Antiviral Research
Donald R Branch
Intravenous immunoglobulin (IVIG) is made from thousands of donors having a variety of blood groups. All of the donors being used for IVIG production, with the exception of group AB donors, have in their plasma antibodies of variable titer commonly known as isohemagglutinins or ABO antibodies. As blood groups O and A are the most commonly found in the world population, most of the plasma used in IVIG production is from donors having these blood groups, with group B and group AB donors being fewer in number...
July 2015: Transfusion
Jun Li, Jian-Li Shi, Xiao-Yan Wu, Fang Fu, Jiang Yu, Xiao-Yuan Yuan, Zhe Peng, Xiao-Yan Cong, Shao-Jian Xu, Wen-Bo Sun, Kai-Hui Cheng, Yi-Jun Du, Jia-Qiang Wu, Jin-Bao Wang, Bao-Hua Huang
Nowadays, adjuvant is still important for boosting immunity and improving resistance in animals. In order to boost the immunity of porcine circovirus type 2 (PCV2) DNA vaccine, CpG motifs were inserted. In this study, the dose-effect was studied, and the immunity of PCV2 DNA vaccines by recombinant open reading frame 2 (ORF2) gene and CpG motifs was evaluated. Three-week-old Changbai piglets were inoculated intramuscularly with 200 μg, 400 μg, and 800 μg DNA vaccines containing 14 and 18 CpG motifs, respectively...
June 2015: Viral Immunology
Guiliang Hao, Libin Wang, Dongmei Chen, Xueyun Liang, Qiong Wang, Yongzhao Zhu, Xiaona Ma, Xiaoming Liu, Yukui Li
OBJECTIVE: To compare the immunosuppressive effects of maternal and fetal placental mesenchymal stem cells (mPMSCs and fPMSCs, respectively) on the rejection of allogenic skin transplants in mice, and further to investigate the mechanism underlying this suppression. METHODS: The mPMSCs and fPMSCs were isolated from human term placentas. The expressions of cell surface markers were detected by flow cytometry. Cell proliferation capacity was characterized by MTT colorimetric assay...
May 2015: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Nobuo Miyazaki, Norihiko Kiyose, Yoko Akazawa, Mizuki Takashima, Yosihisa Hagihara, Naokazu Inoue, Tomonari Matsuda, Ryu Ogawa, Seiya Inoue, Yuji Ito
The antigen-binding domain of camelid dimeric heavy chain antibodies, known as VHH or Nanobody, has much potential in pharmaceutical and industrial applications. To establish the isolation process of antigen-specific VHH, a VHH phage library was constructed with a diversity of 8.4 × 10(7) from cDNA of peripheral blood mononuclear cells of an alpaca (Lama pacos) immunized with a fragment of IZUMO1 (IZUMO1PFF) as a model antigen. By conventional biopanning, 13 antigen-specific VHHs were isolated. The amino acid sequences of these VHHs, designated as N-group VHHs, were very similar to each other (>93% identity)...
September 2015: Journal of Biochemistry
Mervat ElAnsary, Mariam Onsy F Hanna, Gamal Saadi, Mostafa ElShazly, Fatina I Fadel, Hanan AbdElAziz Ahmed, Amr Mostafa Aziz, Amal ElSharnouby, Mona MohiElDin T Kandeel
The increasing demand for solid organs has necessitated the use of ABO and Rhesus (Rh) D minor mismatched transplants. The passenger lymphocyte syndrome (PLS) occurs when donor lymphocytes produce antibodies that react with host red blood cell (RBC) antigens and result in hemolysis. Our aim was to evaluate prospectively the role of PLS in post transplant anemia and hemolysis in ABO and RhD minor mismatched recipients of liver and kidney grafts and to study the association of PLS with donor lymphocyte microchimerism...
June 2015: Human Immunology
M Monfort, P Honoré, A Gothot, C Gérard
BACKGROUND: If 'passenger lymphocyte syndrome' (PLS) is a well-recognized complication in ABO-mismatched solid organ transplantation, the coexistence of this reaction with recipient's alloimmunization against multiple antigens expressed on the residual red blood cells in the graft is less common and unpredictable. METHODS: The receiver of an ABO minor-mismatch liver graft from a cadaveric donor developed haemolytic anaemia within 2 weeks after transplantation. The organ donor was of blood group O D+C+c+E+e+ K+k+ Le(a+b-) and the recipient, A1 D-C-c+E-e+ K-k+ Le(a-b-)...
July 2015: Vox Sanguinis
Małgorzata Leźnicka, Krzysztof Gierlotkal, Tomasz Prycel
BACKGROUND: The objective of the study was to collect the data on undetected hepatitis C virus (HCV) in the frequently hospitalized residents of the Kujawsko-Pomorskie Voivodeship. MATERIAL AND METHODS: The analysis focused on empirical data. The research was conducted in 2013 among 6332 citizens of the Kujawsko-Pomorskie Voivodeship who had been hospitalized at least twice. The blood was tested for the presence of anti-HCV antibodies. The diagnostic survey with an anonymous questionnaire was developed and used for the purpose of the study...
2014: Medycyna Pracy
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