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https://www.readbyqxmd.com/read/29458012/klf10-gene-expression-modulates-fibrosis-in-dystrophic-skeletal-muscle
#1
Joseph X DiMario
Dystrophic skeletal muscle is characterized by fibrotic accumulation of extracellular matrix components that compromise muscle structure, function, and capacity for regeneration. Tissue fibrosis is often initiated and sustained through transforming growth factor β (TGFβ) signaling, and KLF10 is an immediate early gene that is transcriptionally activated in response to TGFβ signaling. It encodes a transcriptional regulator that mediates the effects of TGFβ signaling in a variety of cell types. This report presents results of investigation of the effects of loss of KLF10 gene expression in wild-type and dystrophic (mdx) skeletal muscle...
February 16, 2018: American Journal of Pathology
https://www.readbyqxmd.com/read/29456676/-cordyceps-sinensis-inhibits-airway-remodeling-in-rats-with-chronic-obstructive-pulmonary-disease
#2
Lei Yang, Xingai Jiao, Jinxiang Wu, Jiping Zhao, Tian Liu, Jianfeng Xu, Xiaohui Ma, Liuzao Cao, Lin Liu, Yahui Liu, Jingyu Chi, Minfang Zou, Shuo Li, Jiawei Xu, Liang Dong
Cordyceps sinensis is a traditional Chinese herbal medicine that has been used for centuries in Asia as a tonic to soothe the lung for the treatment of respiratory diseases. The aim of the present study was to determine the effects of C. sinensi s on airway remodeling in chronic obstructive pulmonary disease (COPD) and investigate the underlying molecular mechanisms. Rats with COPD were orally administered C. sinensis at low, moderate or high doses (2.5, 5 or 7.5 g/kg/day, respectively) for 12 weeks. Airway tissue histopathology, lung inflammation and airway remodeling were evaluated...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29436636/effects-of-bradykinin-on-tgf%C3%A2-%C3%AE-1%C3%A2-induced-epithelial%C3%A2-mesenchymal-transition-in-arpe%C3%A2-19-cells
#3
Qingquan Wei, Qingyu Liu, Chengda Ren, Junling Liu, Wenting Cai, Meijiang Zhu, Huizi Jin, Mengmei He, Jing Yu
The aim of the present study was to investigate the effects of bradykinin (BK) on an epithelial-mesenchymal transition (EMT) model in retinal pigment epithelium (RPE) cells through exposure to transforming growth factor‑β1 (TGF‑β1). The aim was to improve the effect of BK on proliferative vitreoretinopathy (PVR) progression, and to find a novel method of clinical prevention and treatment for PVR. The morphology of ARPE‑19 cells was observed using an inverted phase‑contrast microscope. A Cell Counting Kit‑8 was used to assess the effects of TGF‑β1 on the proliferation of ARPE‑19 cells...
February 2, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29436600/adenovirus-mediated-p311-ameliorates-renal-fibrosis-through-inhibition-of-epithelial-mesenchymal-transition-via-tgf-%C3%AE-1-smad-ilk-pathway-in-unilateral-ureteral-obstruction-rats
#4
Fang-Hua Qi, Ping-Ping Cai, Xiang Liu, Guo-Min Si
Epithelial-mesenchymal transition (EMT) is a critical step and key factor during renal fibrosis. Preventing renal tubular EMT is important for delaying the progression of chronic kidney disease (CKD). P311, a highly conserved 8-kDa intracellular protein, has been indicated as an important factor in myofibroblast transformation and in the progression of fibrosis. However, the related studies on P311 on renal fibrosis are limited and the mechanisms of P311 in the progression of renal tubulointerstitial fibrosis remain largely unknown...
February 12, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29434689/lower-energy-radial-shock-wave-therapy-improves-characteristics-of-hypertrophic-scar-in-a-rabbit-ear-model
#5
Jing-Chun Zhao, Bo-Ru Zhang, Kai Shi, Jian Wang, Qing-Hua Yu, Jia-Ao Yu
The aim of the present study was to investigate the effects of radial extracorporeal shock wave therapy (rESWT) on scar characteristics and transforming growth factor (TGF)-β1/Smad signaling in order to explore a potential modality for the treatment of hypertrophic scars (HS). The HS model was generated in rabbit ears, then rabbits were randomly divided into 3 groups: Lower (L)-ESWT [treated with rESWT with lower energy flux density (EFD) of 0.1 mJ/mm2], higher (H)-ESWT (treated with a higher EFD of 0.18 mJ/mm2) and the sham ESWT group (S-ESWT; no ESWT treatment)...
January 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29433423/microrna-17-5p-aggravates-lipopolysaccharide-induced-injury-in-nasal-epithelial-cells-by-targeting-smad7
#6
Nan Huang, Wenjing Li, Xiaolong Wang, Shanshan Qi
BACKGROUND: Globally, rhinitis is one of the most common chronic disorders. Despite availability of drugs to manage the symptomatology of rhinitis, researchers still focus on identification of novel molecular targets for better management. MicroRNAs are implicated in many biological and pathological processes. However, the role of miR-17-5p in rhinitis remains unexplored. This study aimed to explore the role of miR-17-5p in lipopolysaccharide (LPS)-induced injury of nasal epithelial RPMI2650 cells and to elucidate the possible underlying molecular mechanism...
February 13, 2018: BMC Cell Biology
https://www.readbyqxmd.com/read/29415065/high-smad7-and-p-smad2-3-expression-is-associated-with-environmental-enteropathy-in-children
#7
Sana Syed, Vincenzo Dinallo, Najeeha T Iqbal, Laura Di Iorio, Davide Di Fusco, Shan Guleria, Beatrice C Amadi, Kamran Sadiq, Christopher Moskaluk, S Asad Ali, Paul Kelly, Giovanni Monteleone
Enteropathies such as Crohn's disease are associated with enteric inflammation characterized by impaired TGF-β signaling, decreased expression of phosphorylated (p)-SMAD2,3 and increased expression of SMAD7 (an inhibitor of SMAD3 phosphorylation). Environmental enteropathy (EE) is an acquired inflammatory disease of the small intestine (SI), which is associated with linear growth disruption, cognitive deficits, and reduced oral vaccine responsiveness in children <5 y in resource-poor countries. We aimed to characterize EE inflammatory pathways by determining SMAD7 and p-SMAD2,3 levels (using Western blotting) in EE duodenal biopsies (N = 19 children, 7 from Pakistan, 12 from Zambia) and comparing these with healthy controls (Ctl) and celiac disease (CD) patients from Italy...
February 7, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29381406/infection-with-enteric-pathogens-salmonella-typhimurium-and-citrobacter-rodentium-modulate-tgf-beta-smad-signaling-pathways-in-the-intestine
#8
Yong-Guo Zhang, Megha Singhal, Zhijie Lin, Christopher Manzella, Anoop Kumar, Waddah A Alrefai, Pradeep K Dudeja, Seema Saksena, Jun Sun, Ravinder K Gill
Salmonella and Citrobacter are gram negative, members of Enterobacteriaceae family that are important causative agents of diarrhea and intestinal inflammation. TGF-β1 is a pleiotropic multifunctional cytokine that has been implicated in modulating the severity of microbial infections. How these pathogens alter the TGF-β1 signaling pathways in the intestine is largely unknown. Streptomycin-pretreated C57BL/6J mouse model colonized with S. typhimurium for 8 hours (acute) and 4 days (chronic) infection and FVB/N mice infected with C...
January 30, 2018: Gut Microbes
https://www.readbyqxmd.com/read/29375271/microrna-10b-regulates-epithelial-mesenchymal-transition-by-modulating-klf4-klf11-smads-in-hepatocellular-carcinoma
#9
Gulibaha Hujie, Sheng-Hua Zhou, Hua Zhang, Jie Qu, Xiao-Wei Xiong, Outikuer Hujie, Cheng-Gong Liao, Shun-E Yang
Background: Our previous work showed that miR-10b was overexpressed in hepatocellular carcinoma (HCC) and promoted HCC cell migration and invasion. Epithelial-mesenchymal transition (EMT) is involved in HCC metastasis. So, we suspected that miR-10b might participate in the HCC EMT. Methods: We performed morphological analysis and immunofluorescence to observe the roles of miR-10b in HCC EMT. The expression of KLF11 and EMT markers were detected by real-time RT-PCR and western blot...
2018: Cancer Cell International
https://www.readbyqxmd.com/read/29371237/cell-specific-responses-to-the-cytokine-tgf%C3%AE-are-determined-by-variability-in-protein-levels
#10
Jette Strasen, Uddipan Sarma, Marcel Jentsch, Stefan Bohn, Caibin Sheng, Daniel Horbelt, Petra Knaus, Stefan Legewie, Alexander Loewer
The cytokine TGFβ provides important information during embryonic development, adult tissue homeostasis, and regeneration. Alterations in the cellular response to TGFβ are involved in severe human diseases. To understand how cells encode the extracellular input and transmit its information to elicit appropriate responses, we acquired quantitative time-resolved measurements of pathway activation at the single-cell level. We established dynamic time warping to quantitatively compare signaling dynamics of thousands of individual cells and described heterogeneous single-cell responses by mathematical modeling...
January 25, 2018: Molecular Systems Biology
https://www.readbyqxmd.com/read/29368634/binding-and-entry-of-peste-des-petits-ruminants-virus-into-caprine-endometrial-epithelial-cells-profoundly-affect-early-cellular-gene-expression
#11
Bo Yang, Xuefeng Qi, Zhijie Chen, Shuying Chen, Qinghong Xue, Peilong Jia, Ting Wang, Jingyu Wang
Peste des petits ruminants virus (PPRV), the etiological agent of peste des petits ruminants (PPR), causes an acute or subacute disease in small ruminants. Although abortion is observed in an unusually large proportion of pregnant goats during outbreaks of PPR, the pathogenic mechanism underlying remains unclear. Here, the gene expression profile of caprine endometrial epithelial cells (EECs) infected with PPRV Nigeria 75/1 was determined by DNA microarray to investigate the cellular response immediately after viral entry...
January 24, 2018: Veterinary Research
https://www.readbyqxmd.com/read/29366786/smad7-methylation-as-a-novel-marker-in-atherosclerosis
#12
Lihua Wei, Shimei Zhao, Gaoxing Wang, Shiming Zhang, Weibo Luo, Zhengxue Qin, Xiongjie Bi, Yimei Tan, Moke Meng, Jun'an Qin, Huiping Qin, Dan Tian, An'wen Zhang
Atherosclerosis is a complicated process comprising inflammation, accumulation of collagen matrix and aberrant DNA methylation. SMAD7 is known to play an important role in fibrosis and inflammation. In recent years, increasing research has concentrated on the connection between DNA methylation and atherosclerosis. The current study was designed to investigate methylation status of some specific gene with a focus on SMAD7 in atherosclerosis and elucidate their relationship. We found that SMAD7 expression was decreased and its promoter region was markedly methylated in atherosclerotic plaques when compared with normal artery walls...
January 20, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29361889/the-role-and-molecular-mechanism-of-long-nocoding-rna-meg3-in-the-pathogenesis-of-preeclampsia
#13
Lin Yu, Li-Yun Kuang, Fang He, Li-Li Du, Qiu-Lian Li, Wen Sun, Yan-Mei Zhou, Xiao-Mei Li, Xiu-Ying Li, Dun-Jin Chen
A growing body of evidence suggests that the dysregulation of long noncoding RNA is increasingly linked to many human diseases. Maternally expressed gene 3 ( MEG3) is one such gene thought to be affected. In the placenta of patients with preeclampsia, there is reduced expression of MEG3; however, its role and the mechanism involved are not clear. Therefore, we examined the expression of MEG3, epithelial-mesenchymal transition (EMT) markers (E-cadherin and N-cadherin), and TGF-β/smad signaling pathway genes ( TGF-β1, smad3, and smad7) in the placental tissues of 20 patients with preeclampsia and 20 healthy patients...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29361523/the-mir-21-pdcd4-ap-1-feedback-loop-function-as-a-driving-force-for-renal-fibrogenesis
#14
Qi Sun, Jiao Miao, Jing Luo, Qi Yuan, Hongdi Cao, Weifang Su, Yang Zhou, Lei Jiang, Li Fang, Chunsun Dai, Ke Zen, Junwei Yang
Renal fibrosis is a final common pathway of chronic kidney disease. Sustained activation of fibroblast is considered to play a key role in perpetuating renal fibrosis, but the driving force in the perpetuation stage is only partially understood. To date, some investigations have identified specific overexpression of miR-21 in the progression of kidney fibrosis. Nevertheless, the precise role of miR-21 in fibroblast activation remains largely unknown. In this study, we identified miR-21 was significantly upregulated in activated fibroblasts, and maintained itself at constant high level by employing a miR-21/PDCD4/AP-1 auto-regulatory loop...
January 19, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/29350343/syringic-acid-a-phenolic-acid-promotes-osteoblast-differentiation-by-stimulation-of-runx2-expression-and-targeting-of-smad7-by-mir-21-in-mouse-mesenchymal-stem-cells
#15
B Arumugam, K Balagangadharan, N Selvamurugan
Syringic acid (SA), a phenolic acid, has been used in Chinese and Indian medicine for treating diabetes but its role in osteogenesis has not yet been investigated. In the present study, at the molecular and cellular levels, we evaluated the effects of SA on osteoblast differentiation. At the cellular level, there was increased alkaline phosphatase (ALP) activity and calcium deposition by SA treatment in mouse mesenchymal stem cells (mMSCs). At the molecular level, SA treatment of these cells stimulated expression of Runx2, a bone transcription factor, and of osteoblast differentiation marker genes such as ALP, type I collagen, and osteocalcin...
January 19, 2018: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/29330836/pathogenesis-of-non-alcoholic-fatty-liver-disease-mediated-by-yap
#16
Ping Chen, Qihui Luo, Chao Huang, Qi Gao, Like Li, Jingfei Chen, Bing Chen, Wentao Liu, Wen Zeng, Zhengli Chen
OBJECTIVE: This study aimed to investigate the mechanism of the interaction between Yes-associated protein (YAP) and transforming growth factor-β (TGF-β)/Smad signaling pathways in the development of non-alcoholic fatty liver disease (NAFLD). METHODS: Serum samples of monkeys with biopsy-proven NAFLD and healthy normal monkeys were used to measure fasting plasma glucose (FPG), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglyceride (TG) and albumin (ALB) with the BECKMAN CX5 PRO...
January 12, 2018: Hepatology International
https://www.readbyqxmd.com/read/29327472/mesenchymal-stem-cell-deficiency-influences-megakaryocytopoiesis-through-the-tnfaip3-nf-%C3%AE%C2%BAb-smad-pathway-in-patients-with-immune-thrombocytopenia
#17
Yun He, Lin-Lin Xu, Fei-Er Feng, Qian-Ming Wang, Xiao-Lu Zhu, Chen-Cong Wang, Jia-Min Zhang, Hai-Xia Fu, Lan-Ping Xu, Kai-Yan Liu, Xiao-Jun Huang, Xiao-Hui Zhang
Immune thrombocytopenia (ITP) is an autoimmune disease. Mesenchymal stem cells (MSCs) play important roles in the physiology and homeostasis of the haematopoietic system, including supporting megakaryocytic differentiation from CD34+ haematopoietic progenitor cells. Tumour necrosis factor alpha-induced protein 3 (TNFAIP3, also termed A20) plays a key role in terminating NF-κB signalling. Human genetic studies showed that the polymorphisms of the TNFAIP3 gene may contribute to ITP susceptibility. In this study, we showed a significant decrease in TNFAIP3 and increase in NF-κB/SMAD7 in ITP-MSCs...
January 12, 2018: British Journal of Haematology
https://www.readbyqxmd.com/read/29319157/mir-181b-induced-smad7-downregulation-controls-granulosa-cell-apoptosis-through-tgf-%C3%AE-signaling-by-interacting-with-the-tgfbr1-promoter
#18
Wang Yao, Zengxiang Pan, Xing Du, Jinbi Zhang, Qifa Li
SMAD7 disrupts the TGF-β signaling pathway by influencing TGFBR1 stability and by blocking the binding of TGFBR1 to SMAD2/3. In this study, we showed that SMAD7 attenuated the TGF-β signaling pathway in ovarian granulosa cells (GCs) by regulating TGFBR1 transcriptional activity. To function as a transcription factor, SMAD7 downregulated the mRNA levels of TGFBR1 via direct binding to the SMAD-binding elements (SBEs) within the promoter region of pig TGFBR1. We also showed that SMAD7 enhanced porcine GC apoptosis by interrupting TGFBR1 and the TGF-β signaling pathway...
January 10, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29298840/fibronectin-type-iii-and-intracellular-domains-of-toll-like-receptor-4-interactor-with-leucine-rich-repeats-tril-are-required-for-developmental-signaling
#19
Hyung-Seok Kim, Autumn McKnite, Yuanyuan Xie, Jan L Christian
Toll-like receptor 4 interactor with leucine-rich repeats (Tril) functions as a coreceptor for Toll-like receptors (Tlrs) to mediate innate immune responses in adults. In embryos, Tril signals to promote degradation of the Bmp inhibitor, Smad7, to allow for blood formation. It is not known if this function requires, or is independent of Tlrs. In the current studies, we performed a structure-function analysis, which indicated that the fibronectin type III (FN) domain, and the intracellular domain of Tril are required to trigger Smad7 degradation in Xenopus embryos...
January 3, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29297384/upregulation-of-neto2-gene-in-colorectal-cancer
#20
Maria S Fedorova, Anastasiya V Snezhkina, Elena A Pudova, Ivan S Abramov, Anastasiya V Lipatova, Sergey L Kharitonov, Asiya F Sadritdinova, Kirill M Nyushko, Kseniya M Klimina, Mikhail M Belyakov, Elena N Slavnova, Nataliya V Melnikova, Maria A Chernichenko, Dmitry V Sidorov, Marina V Kiseleva, Andrey D Kaprin, Boris Y Alekseev, Alexey A Dmitriev, Anna V Kudryavtseva
BACKGROUND: Neuropilin and tolloid-like 2 (NETO2) is a single-pass transmembrane protein that has been shown primarily implicated in neuron-specific processes. Upregulation of NETO2 gene was also detected in several cancer types. In colorectal cancer (CRC), it was associated with tumor progression, invasion, and metastasis, and seems to be involved in epithelial-mesenchymal transition (EMT). However, the mechanism of NETO2 action is still poorly understood. RESULTS: We have revealed significant increase in the expression of NETO2 gene and deregulation of eight EMT-related genes in CRC...
December 28, 2017: BMC Genetics
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