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Biomaterials vocal fold

Linqing Li, Jeanna M Stiadle, Hang K Lau, Aidan B Zerdoum, Xinqiao Jia, Susan L Thibeault, Kristi L Kiick
Vocal folds are soft laryngeal connective tissues with distinct layered structures and complex multicomponent matrix compositions that endow phonatory and respiratory functions. This delicate tissue is easily damaged by various environmental factors and pathological conditions, altering vocal biomechanics and causing debilitating vocal disorders that detrimentally affect the daily lives of suffering individuals. Modern techniques and advanced knowledge of regenerative medicine have led to a deeper understanding of the microstructure, microphysiology, and micropathophysiology of vocal fold tissues...
November 2016: Biomaterials
Neda Latifi, Hossein K Heris, Scott L Thomson, Rani Taher, Siavash Kazemirad, Sara Sheibani, Nicole Y K Li-Jessen, Hojatollah Vali, Luc Mongeau
The human vocal folds (VFs) undergo complex biomechanical stimulation during phonation. The aim of the present study was to develop and validate a phono-mimetic VF flow perfusion bioreactor, which mimics the mechanical microenvironment of the human VFs in vitro. The bioreactor uses airflow-induced self-oscillations, which have been shown to produce mechanical loading and contact forces that are representative of human phonation. The bioreactor consisted of two synthetic VF replicas within a silicone body. A cell-scaffold mixture (CSM) consisting of human VF fibroblasts, hyaluronic acid, gelatin, and a polyethylene glycol cross-linker was injected into cavities within the replicas...
September 2016: Tissue Engineering. Part C, Methods
Yeji Kim, Emily E Gill, Julie C Liu
Protein-based biomaterials have received significant attention for tissue engineering applications. For example, resilin-based protein gels have been produced with different cross-linking chemistries for applications in cartilage, cardiovascular, and vocal fold engineering. In this study, we investigate an alternative cross-linking chemistry to form resilin-based protein hydrogels and demonstrate the versatility of the gels for investigating cell response to matrix stiffness. Specifically, transglutaminase was used to cross-link proteins and resulted in gel surfaces more suitable for long-term cell attachment compared to those formed by a Mannich-type condensation reaction...
August 8, 2016: Biomacromolecules
Emily A Wrona, Robert Peng, Milan R Amin, Ryan C Branski, Donald O Freytes
The vocal folds (VFs) are exposed to a number of injurious stimuli that frequently lead to aberrant structural alterations and altered biomechanical properties that clinically manifest as voice disorders. Therapies to restore both structure and function of this delicate tissue are ideal. However, such methods have not been adequately developed. Our group and others hypothesize that tissue engineering and regenerative medicine approaches, previously described for other tissue systems, hold significant promise for the VFs...
December 2016: Tissue Engineering. Part B, Reviews
Joo Hyun Woo, Suzanne N King, Henry Hoffman, Seth Dailey, Sarah Wang, Michael B Christensen, Susan L Thibeault
OBJECTIVE: Cells, scaffold, and surgical approaches are important for regeneration of the lamina propria of the scarred vocal fold (VF). Microendoscopy of Reinke's space (MERS) is a surgical approach used to access the lamina propria. The present study evaluated MERS in the treatment of VF scarring as compared with standardized approaches for the treatment of VF scarring with adipose stem cell constructs. STUDY DESIGN: Animal study. SETTING: Academic center...
October 2016: Otolaryngology—Head and Neck Surgery
Behnaz Aghaei-Ghareh-Bolagh, Suzanne M Mithieux, Anthony S Weiss
The elastomeric proteins elastin and resilin have been used extensively in the fabrication of biomaterials for tissue engineering applications due to their unique mechanical and biological properties. Tropoelastin is the soluble monomer component of elastin. Tropoelastin and resilin are both highly elastic with high resilience, substantial extensibility, high durability and low energy loss, which makes them excellent candidates for the fabrication of elastic tissues that demand regular and repetitive movement like the skin, lung, blood vessels, muscles and vocal folds...
June 2016: Current Opinion in Biotechnology
Hossein K Heris, Jamal Daoud, Sara Sheibani, Hojatollah Vali, Maryam Tabrizian, Luc Mongeau
On page 255, M. Tabrizian, L. Mongeau, and co-workers show how human fibroblast cells establish effective adhesion to a scaffold biomaterial consisting of crosslinked hyaluronic acid-gelatin composite microgels. The higher magnification image shows the connection of β1 integrin with the cells' actin filaments and with the microgels' gelatin molecules. Integrins used in the cover art were made from modifications to 2VDO and 3FCS structures from protein data bank.
January 2016: Advanced Healthcare Materials
Linqing Li, Atsushi Mahara, Zhixiang Tong, Eric A Levenson, Christopher L McGann, Xinqiao Jia, Tetsuji Yamaoka, Kristi L Kiick
The outstanding elasticity, excellent resilience at high-frequency, and hydrophilic capacity of natural resilin have motivated investigations of recombinant resilin-based biomaterials as a new class of bio-elastomers in the engineering of mechanically active tissues. Accordingly, here the comprehensive characterization of modular resilin-like polypeptide (RLP) hydrogels is presented and their suitability as a novel biomaterial for in vivo applications is introduced. Oscillatory rheology confirmed that a full suite of the RLPs can be rapidly cross-linked upon addition of the tris(hydroxymethyl phosphine) cross-linker, achieving similar in situ shear storage moduli (20 k ± 3...
January 21, 2016: Advanced Healthcare Materials
Hossein K Heris, Jamal Daoud, Sara Sheibani, Hojatollah Vali, Maryam Tabrizian, Luc Mongeau
The potential use of a novel scaffold biomaterial consisting of cross-linked hyaluronic acid (HA)-gelatin (Ge) composite microgels is investigated for use in treating vocal fold injury and scarring. Cell adhesion integrins and kinematics of cell motion are investigated in 2D and 3D culture conditions, respectively. Human vocal fold fibroblast (hVFF) cells are seeded on HA-Ge microgels attached to a HA hydrogel thin film. The results show that hVFF cells establish effective adhesion to HA-Ge microgels through the ubiquitous expression of β1 integrin in the cell membrane...
January 21, 2016: Advanced Healthcare Materials
(no author information available yet)
Gaston J, Bartlett S, Klemuk SA, Thibeault SL. Formulation and characterization of a porous, elastomeric biomaterial for vocal fold tissue engineering research. Ann Otol Rhinol Laryngol. 2014;123(12):866-874. (Original DOI: 10.1177/0003489414539131) In the "Conclusion" section of the abstract, the sentence "Twenty-µm diameter pores are ideal for cell seeding, and a range of mechanical properties indicates that the higher mass concentration PEU formulations are best suited for mimicking the viscoelastic properties of vocal fold tissue for in vitro research...
August 2015: Annals of Otology, Rhinology, and Laryngology
Aaron M Kosinski, M Preeti Sivasankar, Alyssa Panitch
The impact of RGD integrin binding-peptide concentration and cell phenotype on directing extracellular matrix (ECM) gene expression in vocal fold fibroblasts is little understood. Less is known about cell response to RGD concentration on a biomaterial when fibroblasts are in a scar-like environment compared to a healthy environment. We investigated the effects of varying RGD integrin-binding peptide surface concentration on ECM gene expression of elastin, collagen type 3 alpha 1, decorin, fibronectin, hyaluronan synthase 2, and collagen type 1 alpha 2 in scarred and unscarred immortalized human vocal fold fibroblasts (I-HVFFs)...
September 2015: Journal of Biomedical Materials Research. Part A
Siavash Kazemirad, Hossein K Heris, Luc Mongeau
Crosslinked injectable hyaluronic acid (HA)-gelatin (Ge) hydrogels have remarkable viscoelastic and biological properties for vocal fold tissue engineering. Patient-specific tuning of the viscoelastic properties of this injectable biomaterial could improve tissue regeneration. The frequency-dependent viscoelasticity of crosslinked HA-Ge hydrogels was measured as a function of the concentration of HA, Ge, and crosslinker. Synthetic extracellular matrix hydrogels were fabricated using thiol-modified HA and Ge, and crosslinked by poly(ethylene glycol) diacrylate...
February 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Jeong-Seok Choi, Songyi Lee, Da Yeon Kim, Young-Mo Kim, Moon Suk Kim, Jae-Yol Lim
A biomaterial derived from porcine small intestinal submucosa (SIS) was used in smart drug delivery and tissue remodeling. SIS suspensions were easily formulated by simple mixing with the drug of choice and formed an in situ gel upon injection into tissues, enabling them to act as protein drug depots. This study was conducted to determine whether functional remodeling of an injured vocal fold (VF) could be achieved by hepatocyte growth factor (HGF)-containing SIS in situ-forming gel after VF injury in a rabbit model...
February 2015: Biomaterials
Amir K Miri
Human vocal folds undergo self-sustaining oscillations during phonation. The phonatory functions of the vocal folds depend on their mechanical properties. This article presents a review of various mechanical testing methods and constitutive models that are currently in use for the characterization of mechanical properties of the vocal fold tissue. Special attention is given to tissue deformation under mechanical loading at different length scales. The wide range of elastic modulus values reported in the literature is discussed and justified using a multiscale perspective...
November 2014: Journal of Voice: Official Journal of the Voice Foundation
Joel Gaston, Rebecca S Bartlett, Sarah A Klemuk, Susan L Thibeault
OBJECTIVE: Biomaterials able to mimic the mechanical properties of vocal fold tissue may be particularly useful for furnishing a 3-dimensional microenvironment allowing for in vitro investigation of cell and molecular responses to vibration. Motivated by the dearth of biomaterials available for use in an in vitro model for vocal fold tissue, we investigated polyether polyurethane (PEU) matrices, which are porous, mechanically tunable biomaterials that are inexpensive and require only standard laboratory equipment for fabrication...
December 2014: Annals of Otology, Rhinology, and Laryngology
Jae Won Choi, Ju Kyeong Park, Jae Won Chang, Da Yeon Kim, Moon Suk Kim, Yoo Seob Shin, Chul-Ho Kim
The purpose of this study is to demonstrate scarless vocal fold (VF) regeneration by using a composite gel composed of small intestine submucosa (SIS) and mesenchymal stem cells (MSCs). A scar was made with an electrocoagulator on both VFs in 24 rabbits, followed by injection of either MSCs, SIS, or MSCs-SIS composite gel in the right side VF, while the left side VF was left untreated. VF scars were evaluated with in vivo fluorescence live imaging system (IFLIS), endoscopy, histology, and videokymography (VKG) after eight weeks...
June 2014: Biomaterials
Amir K Miri, Hossein K Heris, Luc Mongeau, Farhad Javid
It is hypothesized that the bulk viscoelasticity of soft tissues is determined by two length-scale-dependent mechanisms: the time-dependent response of the extracellular matrix (ECM) proteins at the nanometer scale and the biophysical interactions between the ECM solid structure and interstitial fluid at the micrometer scale. The latter is governed by poroelasticity theory assuming free motion of the interstitial fluid within the porous ECM structure. In a recent study (Heris, H.K., Miri, A.K., Tripathy, U...
February 2014: Journal of the Mechanical Behavior of Biomedical Materials
Murat Yildirim, Onur Ferhanoglu, James Kobler, Steven M Zeitels, Adela Ben-Yakar
Toward developing a new method for restoring tissue viscoelasticity in scarred vocal folds, we previously proposed a method to localize biomaterials within subepithelial voids ablated using ultrafast laser pulses. The clinical implementation of this method necessitates the quantification of the laser parameters for ablating scarred tissue. Here, we present a comprehensive study of these parameters including ablation threshold and bubble lifetime in healthy and scarred tissues. We also present a new method for extracting tissue-specific ablation threshold and scattering lengths of different tissue layers...
November 2013: Journal of Biomedical Optics
Aaron Cunningham, Hunter Faircloth, Matthew Jones, Christopher Johnson, Tiffany Coleman, George Wicks, Gregory Postma, Paul Weinberger
OBJECTIVES/HYPOTHESIS: The primary objective was to design a reporter assay to measure molecular release kinetics from a new porous-wall hollow glass microsphere biomaterial with great potential in regenerative medicine and drug delivery. Second, future avenues for research will be discussed specifically in regard to potential clinical uses in laryngology. STUDY DESIGN: Basic science data report. METHODS: We developed an assay using fluorescent nanocrystals or quantum dots (Qdot 605) as a reporter...
June 2014: Laryngoscope
Young-Mo Kim, Se H Oh, Jeong-Seok Choi, Songyi Lee, Jeong C Ra, Jin H Lee, Jae-Yol Lim
OBJECTIVES/HYPOTHESIS: The purpose of this study was to investigate the regenerative efficacy of an injectable hyaluronic acid/mildly cross-linked alginate hydrogel (HA/ALG hydrogel) containing human adipose-derived mesenchymal stem cells (hAdMSCs) for vocal fold (VF) wound healing. STUDY DESIGN: Animal research. METHODS: HA/ALG hydrogel containing hAdMSCs was injected into the VFs of rabbits immediately after direct injury. Endoscopic evaluations were performed at 1 and 3 months after injury, and functional evaluations of mucosal vibration and viscoelastic properties were carried out posteuthanization at 3 months after injury...
March 2014: Laryngoscope
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