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ribosome inactivating protein

Lei Feng, Li Chen, Junwen Yun, Zhixiang Bi, Yinghua Tang, Peipei Wu, Jibo Hou
Inclusion body hepatitis and hydropericardium-hepatitis syndrome caused by serotype 4 fowl adenovirus (FAdV-4) have emerged in China since 2013. FAdV is usually propagated in primary chicken embryonic liver cells or embryo yolk sac. The aim of this work was to develop an immortalized CEL cell line by stable expression of human mitochondrial ribosomal protein 18S-2, named CEL-hMRP18S-2 cells, for the propagation of FAdV-4. The maximum cell density of CEL-hMRP18S-2 cells could reach 2.65 × 106  cells/ml in four-days culture...
May 8, 2018: Biologicals: Journal of the International Association of Biological Standardization
Elton Chaves, Itácio Queiroz de Mello Padilha, Demetrius Antônio Machado Araújo, Gerd Bruno Rocha
Ricin is a ribosome-inactivating protein (RIP-type2) consisting of two subunits, Ricin Toxin A (RTA) and Ricin Toxin B (RTB). Due to its cytotoxicity, ricin has worried world authorities for its potential use as a chemical weapon; therefore, its inhibition is of great biotechnological interest. RTA is the target for inhibitor synthesis and pterin derivatives are promising candidates to inhibit it. In this study, we used a combination of molecular docking approach and fast steered molecular dynamics in order to assess the correlation between non-equilibrium work, 〈W〉, and IC50 of six RTA inhibitors...
May 11, 2018: Journal of Chemical Information and Modeling
Bin Zhang, Bang-Ce Ye
5-Aminolevulinic acid (ALA) is a non-protein amino acid with a significant potential for cancer treatment and plant stress resistance. Microbial fermentation has gradually replaced the traditional chemical-based method for ALA production, thus increasing the need for high-ALA-producing strains. In this study, we engineered the glutamate producing strain, Corynebacterium glutamicum S9114, for ALA production. To efficiently convert l-glutamate to ALA, hemA and hemL from Salmonella typhimurium and Escherichia coli were tandemly overexpressed...
May 2018: 3 Biotech
Stephanie Biedka, Jelena Micic, Daniel Wilson, Hailey Brown, Luke Diorio-Toth, John L Woolford
Ribosome biogenesis involves numerous preribosomal RNA (pre-rRNA) processing events to remove internal and external transcribed spacer sequences, ultimately yielding three mature rRNAs. Removal of the internal transcribed spacer 2 spacer RNA is the final step in large subunit pre-rRNA processing and begins with endonucleolytic cleavage at the C2 site of 27SB pre-rRNA. C2 cleavage requires the hierarchical recruitment of 11 ribosomal proteins and 14 ribosome assembly factors. However, the function of these proteins in C2 cleavage remained unclear...
April 24, 2018: Journal of Cell Biology
Lingyu Zhu, Suisui Zhang, Xuejie Huan, Yan Mei, Huawei Yang
Ribosomal S6 protein kinase 4 (RSK4) was known as a novel tumor suppressor gene, and the tumor necrosis factor receptor-associated factor 4 (TRAF4) was linked to carcinogenesis. The purpose of this study is to further investigate the effect of the TRAF4 gene on cell proliferation, invasion and metastasis in vivo and explore whether there is an interaction between TRAF4 and RSK4 in breast cancer. MDA-MB-231 cells were transfected with lentivirus TRAF4-shRNA to specifically block the expression of TRAF4, or transfected with lentivirus negative-shRNA as a negative control...
April 20, 2018: Biochemical and Biophysical Research Communications
Elio Pizzo, Katia Pane, Andrea Bosso, Nicola Landi, Sara Ragucci, Rosita Russo, Rosa Gaglione, Marcelo D T Torres, Cesar de la Fuente-Nunez, Angela Arciello, Alberto Di Donato, Eugenio Notomista, Antimo Di Maro
Antimicrobial peptides, also called Host Defence Peptides (HDPs), are effectors of innate immune response found in all living organisms. In a previous report, we have identified by chemical fragmentation, and characterized the first cryptic antimicrobial peptide in PD-L4, a type 1 ribosome-inactivating protein (RIP) from leaves of Phytolacca dioica L. We applied a recently developed bioinformatic approach to a further member of the differently expressed pool of type 1 RIPs from P. dioica (PD-L1/2), and identified two novel putative cryptic HDPs in its N-terminal domain...
April 20, 2018: Biochimica et Biophysica Acta
Manuel Garrosa, Pilar Jiménez, Damián Córdoba-Díaz, Verónica García-Recio, Sara Gayoso, María Ángeles Rojo, Manuel J Gayoso, Tomás Girbés
Ebulin f is a ribosome-inactivating protein (RIP) present in green fruits of the dwarf elder (Sambucus ebulus L). Since dwarf elder fruits are used for food and as a medicine, we assessed the study of toxicological effects and safety of ebulin f in elderly mice, comparing these results with those reported in young animals and with other RIPs. Female Swiss mice aged 6 and 12 months of age were intraperitoneally injected with a single dose from 1.4 to 4.5 mg/kg ebulin f. Heart, stomach, intestines, lung, kidney, liver, spleen, pancreas, adrenal gland, uterus, ovary and brain were studied...
April 23, 2018: Histology and Histopathology
Julien Barbier, Daniel Gillet
Ribosome inactivating proteins (RIPs) form a vast family of hundreds of toxins from plants, fungi, algae, and bacteria. RIP activities have also been detected in animal tissues. They exert an N-glycosydase catalytic activity that is targeted to a single adenine of a ribosomal RNA, thereby blocking protein synthesis and leading intoxicated cells to apoptosis. In many cases, they have additional depurinating activities that act against other nucleic acids, such as viral RNA and DNA, or genomic DNA. Although their role remains only partially understood, their functions may be related to plant defense against predators and viruses, plant senescence, or bacterial pathogenesis...
April 18, 2018: Toxins
José M Andrade, Ricardo F Dos Santos, Irina Chelysheva, Zoya Ignatova, Cecília M Arraiano
Ribosome biogenesis is a complex process involving multiple factors. Here, we show that the widely conserved RNA chaperone Hfq, which can regulate sRNA-mRNA basepairing, plays a critical role in rRNA processing and ribosome assembly in Escherichia coli Hfq binds the 17S rRNA precursor and facilitates its correct processing and folding to mature 16S rRNA Hfq assists ribosome assembly and associates with pre-30S particles but not with mature 30S subunits. Inactivation of Hfq strikingly decreases the pool of mature 70S ribosomes...
April 18, 2018: EMBO Journal
Tian Jiang, Jing Zhang, Liwei Rong, Yanjiang Feng, Qi Wang, Qiulai Song, Lixin Zhang, Min Ouyang
Chloroplast development is a highly complex process and the regulatory mechanism has not been fully characterized. Identification of the corresponding regulators will shed light on the intricacies of chloroplast development. Here, we identified ECD1 (Early Chloroplast Development 1), a chloroplast-localized pentatricopeptide repeat protein (PPR) belonging to the PLS subfamily. Inactivation of ECD1 leads to embryo lethality, and abnormal embryogenesis occurs in ecd1/+ heterozygous plants. A decrease of ECD1 induced by RNA interference (RNAi) results in seedlings with albino cotyledons but normal true leaves...
April 10, 2018: Journal of Experimental Botany
Sugandh Saxena, Shatrunajay Shukla, Poonam Kakkar
Pleckstrin homology domain leucine-rich repeat protein phosphatase 2 (PHLPP2) has been known to exert tumor suppressive activity for long without much knowledge about its regulation and implications. Protein kinase B (Akt), Protein kinase C (PKC) and Ribosomal protein S6 Kinase (S6K) are known downtargets of PHLPP2, regulating a plethora of life processes viz. cell growth, survival and evasion from apoptosis. Present study decoded the crucial role of PHLPP2 in inducing apoptosis by its interaction with the newly found binding partner Mammalian sterile 20-like kinase 1 (Mst1) in berberine (BBR)-treated human hepatoma cells...
April 4, 2018: Toxicology and Applied Pharmacology
Deasy Pertiwi, Ronny Martien, - Sismindari, Hilda Ismail
Ribosome Inactivating Proteins (RIPs) isolated from Mirabilis jalapa L. (MJ protein) leaves showed high cytotoxic effect on malignant. Chitosan nanoparticles have frequently been used in protein delivery applications. The aim of this study was to develop targeted drug delivery system of RIP MJ for breast cancer therapy with chitosan nanoparticles conjugated antiEpCAM antibody. RIP MJ nanoparticles were prepared using low viscous chitosan and pectin using polyelectrolit complex method, followed by conjugation process with antiEpCAM antibody...
March 2018: Pakistan Journal of Pharmaceutical Sciences
Cuiping Zhang, Ying Liang
PURPOSE OF REVIEW: Hematopoietic stem cells (HSCs) produce mature blood cells throughout lifetime. Natural genetic diversity offers an important yet largely untapped reservoir for deciphering regulatory mechanisms of HSCs and hematopoiesis. In this review, we explore the role of latexin, identified by natural variation, in regulating homeostatic and stress hematopoiesis, unravel the underlying signaling pathways, and propose its therapeutic implication. RECENT FINDINGS: Latexin acts endogenously in HSCs to negatively regulate their population size by enhancing apoptosis and by decreasing self-renewal...
March 31, 2018: Current Opinion in Hematology
Ting Yang, Li-Sha Zhu, Yao Meng, Rui Lv, Zhuo Zhou, Lin Zhu, Hong-Hui Lin, De-Hui Xi
Alpha-momorcharin (α-MMC) is a type-I ribosome inactivating protein (RIP) with a molecular weight of 29 kDa found in plants. This protein has been shown to be effective against a broad range of human viruses and also has anti-tumor activities. However, the mechanism by which α-MMC induces plant defense responses and regulates the N gene to promote resistance to the Tobacco mosaic virus (TMV) is still not clear. By using pharmacological and infection experiments, we found that α-MMC enhances TMV resistance of tobacco plants containing the N gene (tobaccoNN )...
April 29, 2017: Journal of Plant Physiology
Jieyu Wu, Xue Jiang, Yamei Li, Tingting Zhu, Jingjing Zhang, Zhiguo Zhang, Linqiang Zhang, Yuru Zhang, Yanli Wang, Xiaoju Zou, Bin Liang
The primary function of the nucleolus is ribosome biogenesis, which is an extremely energetically expensive process. Failures in ribosome biogenesis cause nucleolar stress with an altered energy status. However, little is known about the underlying mechanism linking nucleolar stress to energy metabolism. Here we show that nucleolar stress is triggered by inactivation of RSKS-1 (ribosomal protein S6 kinase), RRP-8 (ribosomal RNA processing 8), and PRO-2/3 (proximal proliferation), all of which are involved in ribosomal RNA processing or inhibition of rDNA transcription by actinomycin D (AD), leading to excessive lipid accumulation in Caenorhabditis elegans...
March 22, 2018: Nature Communications
Bei Wang, Shu-Sen Zheng, Tan-Yang Zhou, Chun-Hui Nie, Da-Long Wan
Bigelovin (BigV) is a sesquiterpene lactone, isolated from Inula helianthus aquatica, which has been reported to induce apoptosis and show anti-inflammatory and anti-angiogenic activities. Nevertheless, the effects of BigV on liver cancer and the underlying mechanisms have not been investigated. In the study, we found that BigV exhibited potential anti-tumor activities against human liver cancer in vitro and in vivo. BigV reduced the cell proliferation and colony formation. BigV induced apoptosis through improving the cleavage of Caspase-3 and poly (ADP-ribose) polymerase 1 (PARP-1)...
March 13, 2018: Biochemical and Biophysical Research Communications
Ziling Fang, Bo Cao, Jun-Ming Liao, Jun Deng, Kevin D Plummer, Peng Liao, Tao Liu, Wensheng Zhang, Kun Zhang, Li Li, David Margolin, Shelya X Zeng, Jianping Xiong, Hua Lu
Ribosomal proteins (RPs) play important roles in modulating the MDM2-p53 pathway. However, less is known about the upstream regulators of the RPs. Here we identify SPIN1 (Spindlin 1) as a novel binding partner of human RPL5/uL18 that is important for this pathway. SPIN1 ablation activates p53, suppresses cell growth, reduces clonogenic ability, and induces apoptosis of human cancer cells. Mechanistically, SPIN1 sequesters uL18 in the nucleolus, preventing it from interacting with MDM2, and thereby alleviating uL18-mediated inhibition of MDM2 ubiquitin ligase activity towards p53...
March 16, 2018: ELife
Stephanie C Casey, Virginie Baylot, Dean W Felsher
The MYC proto-oncogene is a gene product that coordinates the transcriptional regulation of a multitude of genes that are essential to cellular programs required for normal as well as neoplastic cellular growth and proliferation, including cell cycle, self-renewal, survival, cell growth, metabolism, protein and ribosomal biogenesis, and differentiation. Here, we propose that MYC regulates these programs in a manner that is coordinated with a global influence on the host immune response. MYC had been presumed to contribute to tumorigenesis through tumor cell-intrinsic influences...
May 3, 2018: Blood
Tung-Chen Yeh, Ching-Sen Shin, Hsin-Hung Chen, Chi-Cheng Lai, Gwo-Ching Sun, Ching-Jiunn Tseng, Pei-Wen Cheng
Resveratrol is a polyphenol with pleiotropic effects against oxidative damage that has been widely implicated in the lowering of hypertension risk. The purpose of this study was to determine whether improve NO release in the brain either through the activation of AMPK or reduced Rac1-induced ROS generation, thereby reducing BP in rats with fructose-induced hypertension. The rats were fed with 10% fructose or crestor (rosuvastatin; 1.5 mg/kg/day) and resveratrol (10 mg/kg/day) treatment for 1 week, the systolic blood pressure (SBP) of the rats were measured by tail-cuff method...
March 1, 2018: Journal of Applied Physiology
Bee Gek Ng, Jae Woo Han, Dong Wan Lee, Gyung Ja Choi, Beom Seok Kim
Hahella chejuensis MB-1084 is a Gram-negative marine bacterial strain that produces unusual 17-membered carbocyclic tetraenes, chejuenolide A and B. Two fosmid clones responsible for chejuenolide production were identified from the genomic DNA library of the MB-1084 strain. Systematic inactivation of the open reading frames (ORFs) in the sequenced region defines the boundaries of the chejuenolide (che) biosynthetic gene cluster (24.9 kbp) that encodes one non-ribosomal peptide synthase (NRPS)-polyketide synthase (PKS) hybrid protein, three modular PKSs, two PKS domains, and an amine oxidase homolog...
May 2018: Journal of Antibiotics
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