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ribosome inactivating protein

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https://www.readbyqxmd.com/read/28301598/regulation-of-anti-apoptotic-bcl-2-family-protein-mcl-1-by-s6-kinase-2
#1
Alakananda Basu, Savitha Sridharan
The anti-apoptotic Bcl-2 family protein myeloid cell leukemia-1 (Mcl-1) plays an important role in breast cancer cell survival and chemoresistance. We have previously shown that knockdown of the 40S ribosomal protein S6 kinase-2 (S6K2), which acts downstream of the mechanistic target of rapamycin complex 1 (mTORC1), enhanced breast cancer cell death by apoptotic stimuli. The increase in cell death by S6K2 depletion was partly due to inactivation of Akt. In the present study, we investigated if S6K2 regulates Mcl-1, which acts downstream of Akt...
2017: PloS One
https://www.readbyqxmd.com/read/28288935/evaluation-of-abrin-induced-nephrotoxicity-by-using-novel-renal-injury-markers
#2
Bhavana Sant, P V Lakshmana Rao, D P Nagar, S C Pant, A S B Bhasker
Abrin is a potent plant toxin analogous to ricin that is derived from the seeds of Abrus precatorius plant. It belongs to the family of type II ribosome-inactivating proteins and causes cell death by irreversibly inactivating ribosomes through site-specific depurination. In this study we examined the in vivo nephrotoxicity potential of abrin toxin in terms of oxidative stress, inflammation, histopathological changes and biomarkers of kidney injury. Animals were exposed to 0.5 and 1.0 LD50 dose of abrin by intraperitoneal route and observed for 1, 3, and 7 day post-toxin exposure...
March 10, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28264936/deletion-of-ribosomal-protein-genes-is-a-common-vulnerability-in-human-cancer-especially-in-concert-with-tp53-mutations
#3
Ram Ajore, David Raiser, Marie McConkey, Magnus Jöud, Bernd Boidol, Brenton Mar, Gordon Saksena, David M Weinstock, Scott Armstrong, Steven R Ellis, Benjamin L Ebert, Björn Nilsson
Heterozygous inactivating mutations in ribosomal protein genes (RPGs) are associated with hematopoietic and developmental abnormalities, activation of p53, and altered risk of cancer in humans and model organisms. Here we performed a large-scale analysis of cancer genome data to examine the frequency and selective pressure of RPG lesions across human cancers. We found that hemizygous RPG deletions are common, occurring in about 43% of 10,744 cancer specimens and cell lines. Consistent with p53-dependent negative selection, such lesions are underrepresented in TP53-intact tumors (P ≪ 10(-10)), and shRNA-mediated knockdown of RPGs activated p53 in TP53-wild-type cells...
March 6, 2017: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/28264932/zinc-starvation-induces-autophagy-in-yeast
#4
Tomoko Kawamata, Tetsuro Horie, Miou Matsunami, Michiko Sasaki, Yoshinori Ohsumi
Zinc is an essential nutrient for all forms of life. Within cells, most zinc is bound to protein. Because zinc serves as a catalytic or structural cofactor for many proteins, cells must maintain zinc homeostasis under severely zinc-deficient conditions. In yeast, the transcription factor Zap1 controls the expression of genes required for uptake and mobilization of zinc, but to date the fate of existing zinc-binding proteins under zinc starvation remains poorly understood. Autophagy is an evolutionarily conserved cellular degradation/recycling process in which cytoplasmic proteins and organelles are sequestered for degradation in the vacuole/lysosome...
March 6, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28260497/improved-protein-toxin-delivery-based-on-attempts-systems
#5
Yongzhuo Huang, Victor C Yang, Feng Li, Meong Cheol Shin, Huiyuan Wang, Kyoung Ah Min, Meng Zhang, Yingzhi Chen
Ribosome-inactivating proteins (RIPs) are wildly found in multiple species of plants, bacteria and fungi. As a special family of protein toxins, RIPs can inhibit protein synthesis and induce cell death via inactivating ribosome in eukaryotic cells. Thus, RIPs have been applied for anti-tumor therapy in the past two decades. However, because of poor cell permeability, nonselective mode of action for tumor cells, poor pharmacokinetic profiles and immunogenicity, their clinical application has been severely constrained...
March 1, 2017: Current Drug Targets
https://www.readbyqxmd.com/read/28218711/special-issue-ribosome-inactivating-proteins-commemorative-issue-in-honor-of-professor-fiorenzo-stirpe
#6
EDITORIAL
Els J M Van Damme
The family of ribosome-inactivating proteins (RIPs) groups all enzymes (EC.3.2.2.22) with a so-called RIP domain which comprises N-glycosidase activity and enables these proteins to catalytically inactivate ribosomes.[...].
February 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28147343/the-ribosomal-protein-gene-rpl5-is-a-haploinsufficient-tumor-suppressor-in-multiple-cancer-types
#7
Laura Fancello, Kim R Kampen, Isabel Jf Hofman, Jelle Verbeeck, Kim De Keersmaecker
For many years, defects in the ribosome have been associated to cancer. Recently, somatic mutations and deletions affecting ribosomal protein genes were identified in a few leukemias and solid tumor types. However, systematic analysis of all 81 known ribosomal protein genes across cancer types is lacking. We screened mutation and copy number data of respectively 4926 and 7322 samples from 16 cancer types and identified six altered genes (RPL5, RPL11, RPL23A, RPS5, RPS20 and RPSA). RPL5 was located at a significant peak of heterozygous deletion or mutated in 11% of glioblastoma, 28% of melanoma and 34% of breast cancer samples...
January 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28134797/hyperuricaemia-%C3%A2-xanthine%C3%A2-oxidoreductase%C3%A2-and-ribosome-inactivating%C3%A2-proteins%C3%A2-from%C3%A2-plants-the%C3%A2-contributions%C3%A2-of%C3%A2-fiorenzo%C3%A2-stirpe%C3%A2-to-frontline%C3%A2-research
#8
REVIEW
Andrea Bolognesi, Massimo Bortolotti, Maria Giulia Battelli, Letizia Polito
The enzymes called ribosome-inactivating proteins (RIPs) that are able to depurinate  nucleic acids and arrest vital cellular functions, including protein synthesis, are still a frontline  research field, mostly because of their promising medical applications. The contributions of Stirpe  to the development of these studies has been one of the most relevant. After a short biographical  introduction, an overview is offered of the main results obtained by his investigations during last  55 years on his main research lines: hyperuricaemia, xanthine oxidoreductase and RIPs...
January 27, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28109079/silencing-of-ribosomal-protein-l34-rpl34-inhibits-the-proliferation-and-invasion-of-esophageal-cancer-cells
#9
Huijie Fan, Jing Li, Yongxu Jia, Jingjing Wu, Long Yuan, Mingjun Li, Jiangqi Wei, Benling Xu
Ribosomal protein L34 (RPL34) belongs to the L34E family of ribosomal proteins and contains a zinc finger motif. Aberrant expression of RPL34 has been reported in several human malignancies. However, the precise role and potential underlying mechanisms of RPL34 in human esophageal cancer remain largely unknown. Thus, the objective of this study was to investigate the role of RPL34 in esophageal cancer progression. Our results showed that the expression of RPL34 at both mRNA and protein levels was frequently up-regulated in esophageal cancer cell lines...
January 20, 2017: Oncology Research
https://www.readbyqxmd.com/read/28103015/antimicrobial-activity-ame-resistance-and-a-site-binding-studies-of-anthraquinone-neomycin-conjugates
#10
Natalya N Degtyareva, Changjun Gong, Sandra Story, Nathanael S Levinson, Adegboyega K Oyelere, Keith D Green, Sylvie Garneau-Tsodikova, Dev P Arya
The antibacterial effects of aminoglycosides are based on their association with the A-site of bacterial rRNA and interference with the translational process in the bacterial cell, causing cell death. The clinical use of aminoglycosides is complicated by resistance and side effects, some of which arise from their interactions with the human mitochondrial 12S rRNA and its deafness-associated mutations, C1494U and A1555G. We report a rapid assay that allows screening of aminoglycoside compounds to these classes of rRNAs...
February 17, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28090686/effectively-enhancing-cytotoxic-and-apoptotic-effects-of-alpha-momorcharin-by-integrating-a-heparin-binding-peptide
#11
Meng-Jie Tan, Xue-Wei Cao, Peng-Fei Li, Yi-Zhou Zhai, Yu Zhou, Ye-Jun Liu, Jian Zhao, Fu-Jun Wang
Alpha-momorcharin (α-MMC), a type I ribosome inactivating protein, has attracted a great deal attention because of its antitumor activity. However, the cytotoxicity of α-MMC is limited due to insufficient cellular internalization in cancer cells. To enhance the cytotoxicity of α-MMC, a heparin-binding domain derived from HB-EGF (named HBP) was used as a cell-penetrating peptide and fused to the C-terminus of α-MMC. This novel α-MMC-HBP fusion protein was expressed and purified with a Ni(2+) -resin. The N-glycosidase activity and DNase activity assay indicated that the introduction of HBP did not interfere with the intrinsic bioactivities of α-MMC...
January 16, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28088476/a-chimeric-protein-of-abrin-and-abrus-precatorius-agglutinin-that-neutralizes-abrin-mediated-lethality-in-mice
#12
Vinita Tiwari, Shradha Bagaria, Anjali A Karande
Abrin, a type II ribosome inactivating protein from the Abrus precatorius plant, is extremely toxic. It has been shown to be 75 times more potent than its infamous sister toxin, ricin and their potential use in bio-warfare is a cause of major concern. Although several vaccine candidates are under clinical trials for ricin, none are available against abrin. The present study proposes a chimeric protein, comprising of 1-123 amino acids taken from the A chain of abrin and 124-175 amino acids from Abrus precatorius agglutinin A chain, as a vaccine candidate against abrin intoxication...
January 11, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28067841/lectin-digestibility-and-stability-of-elderberry-antioxidants-to-heat-treatment-in-vitro
#13
Pilar Jiménez, Patricia Cabrero, Damian Cordoba-Diaz, Manuel Cordoba-Diaz, Manuel Garrosa, Tomás Girbés
Elderberry contains healthy low molecular weight nutraceuticals and lectins which are sequence-related to the elderberry allergen Sam n1. Some of these lectins are type II ribosome-inactivating proteins. The sensitivity of native lectins present in elderberry fruits and bark to the proteolysis triggered by in vitro simulated gastric and duodenal fluids has been investigated. It was found that these lectins are refractory to proteolysis. Nonetheless, incubation for 5-10 min in a boiling water bath completely sensitized them to the hydrolytic enzymes in vitro...
January 6, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28029105/cathepsin-b-degradable-nir-responsive-nanoparticulate-platform-for-target-specific-cancer-therapy
#14
Sam P Tarassoli, Alejandra Martinez de Pinillos Bayona, Hayley Pye, C Alexander Mosse, John F Callan, Alexander MacRobert, Anthony P McHale, Nikolitsa Nomikou
Stimuli-responsive anticancer formulations can promote drug release and activation within the target tumour, facilitate cellular uptake, as well as improve the therapeutic efficacy of drugs and reduce off-target effects. In the present work, indocyanine green (ICG)-containing polyglutamate (PGA) nanoparticles were developed and characterized. Digestion of nanoparticles with cathepsin B, a matrix metalloproteinase overexpressed in the microenvironment of advanced tumours, decreased particle size and increased ICG cellular uptake...
February 3, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28028039/draft-genome-sequence-of-bitter-gourd-momordica-charantia-a-vegetable-and-medicinal-plant-in-tropical-and-subtropical-regions
#15
Naoya Urasaki, Hiroki Takagi, Satoshi Natsume, Aiko Uemura, Naoki Taniai, Norimichi Miyagi, Mai Fukushima, Shouta Suzuki, Kazuhiko Tarora, Moritoshi Tamaki, Moriaki Sakamoto, Ryohei Terauchi, Hideo Matsumura
Bitter gourd (Momordica charantia) is an important vegetable and medicinal plant in tropical and subtropical regions globally. In this study, the draft genome sequence of a monoecious bitter gourd inbred line, OHB3-1, was analyzed. Through Illumina sequencing and de novo assembly, scaffolds of 285.5 Mb in length were generated, corresponding to ∼84% of the estimated genome size of bitter gourd (339 Mb). In this draft genome sequence, 45,859 protein-coding gene loci were identified, and transposable elements accounted for 15...
December 27, 2016: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28009354/rsk-mediated-nuclear-accumulation-of-the-cold-shock-y-box-protein-1-controls-proliferation-of-t-cells-and-t-all-blasts
#16
Steffi Gieseler-Halbach, Stefan Meltendorf, Mandy Pierau, Soenke Weinert, Florian H Heidel, Thomas Fischer, Juliane Handschuh, Ruediger C Braun-Dullaeus, Martin Schrappe, Jonathan A Lindquist, Peter R Mertens, Ulrich Thomas, Monika C Brunner-Weinzierl
Deregulated proliferation is key to tumor progression. Although unrestricted proliferation of solid tumor cells correlates with the cold-shock protein Y-box (YB)-binding protein-1 accumulation in the nuclei, little is known about its expression and function in hematopoietic malignancies, such as T-cell acute lymphoblastic leukemia (T-ALL). Here we show that YB-1 protein is highly enriched in the nuclei of activated T cells and malignant human T-ALL cell lines but not in resting T cells. YB-1 S(102) mutations that either mimic (S102D) or prevent phosphorylation (S102N) led to accumulation of YB-1 in the nucleus of T cells or strictly excluded it, respectively...
February 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28003804/golden-needle-mushroom-a-culinary-medicine-with-evidenced-based-biological-activities-and-health-promoting-properties
#17
REVIEW
Calyn Tang, Pearl Ching-Xin Hoo, Loh Teng-Hern Tan, Priyia Pusparajah, Tahir Mehmood Khan, Learn-Han Lee, Bey-Hing Goh, Kok-Gan Chan
Flammulina velutipes (enoki, velvet shank, golden needle mushroom or winter mushroom), one of the main edible mushrooms on the market, has long been recognized for its nutritional value and delicious taste. In recent decades, research has expanded beyond detailing its nutritional composition and delved into the biological activities and potential health benefits of its constituents. Many bioactive constituents from a range of families have been isolated from different parts of the mushroom, including carbohydrates, protein, lipids, glycoproteins, phenols, and sesquiterpenes...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27999336/debouganin-diabody-fusion-protein-overcomes-drug-resistance-to-adcs-comprised-of-anti-microtubule-agents
#18
Shilpa Chooniedass, Rachelle L Dillon, Arjune Premsukh, Peter J Hudson, Gregory P Adams, Glen C MacDonald, Jeannick Cizeau
Antibody drug conjugates (ADC), comprised of highly potent small molecule payloads chemically conjugated to a full-length antibody, represent a growing class of therapeutic agents. The targeting of cytotoxic payloads via the specificity and selectivity of the antibody has led to substantial clinical benefits. However, ADC potency can be altered by mechanisms of resistance such as overexpression of efflux pumps or anti-apoptotic proteins. DeBouganin is a de-immunized variant of bouganin, a ribosome-inactivating protein (RIP) that blocks protein synthesis, thereby leading to apoptosis...
December 17, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27993682/trastuzumab-stimulation-of-ribosomal-protein-s6-kinase-1-s6k1-predicts-de-novo-trastuzumab-resistance
#19
Felicia C Huynh, Daniel Nguyen, Frank E Jones
Breast cancer is a complex disease with at least five different molecular subtypes identified. The breast tumor molecular subtypes guide stratification of patients for specific targeted therapy regimens and each subtype is associated with significantly different patient outcomes. For example, patients with the HER2 positive molecular subtype benefit from the HER2 targeted therapy trastuzumab. Unfortunately, women with the HER2 positive molecular subtype have the worst overall prognosis and nearly 70% of women with HER2 positive breast cancer exhibit de novo or acquired resistance to trastuzumab...
January 29, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27959964/proteomic-analysis-of-eif5b-silencing-modulated-proteostasis
#20
Xu Jiang, Xiaoyong Jiang, Yun Feng, Renhua Xu, Qingtao Wang, Haiteng Deng
Protein translational machinery is an important component of the proteostasis network that maintains cellular proteostasis and regulates aging and other cellular processes. Ample evidence indicates that inhibition of translation initiation factor activities enhances stress resistance in model organisms. Eukaryotic translation initiation factor 5B (eIF5B) acts by joining the pre-40S subunit with the 60S ribosomal unit to form an 80S-like complex during protein translational initiation. Reduced eIF5B expression may disrupt proteostasis and trigger cellular processes associated with stress responses...
2016: PloS One
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