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https://www.readbyqxmd.com/read/27913088/effect-of-suction-pressures-on-cell-yield-and-functionality-of-the-adipose-derived-stromal-vascular-fraction
#1
Yuan-Wen Chen, Jing-Ru Wang, Xuan Liao, Sheng-Hong Li, Li-Ling Xiao, Biao Cheng, Guang-Hui Xie, Jian-Xing Song, Hong-Wei Liu
OBJECTIVE: The present study was performed to explore the effect of suction pressures on the cell yield of adipose-derived stromal vascular fraction (SVF) and the functionality of adipose-derived stem cells (ADSCs), situated in the SVF, and to develop optimal parameters of harvesting SVF for clinical use. METHODS: Adipose tissue was harvested from the lower abdomen of 10 patients by suction-assisted lipoplasty. Suction pressure was either -30 ± 5 kPa or -55 ± 5 kPa...
November 11, 2016: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/27902739/degenerative-suspensory-ligament-desmitis-dsld-in-peruvian-paso-horses-is-characterized-by-altered-expression-of-tgf%C3%AE-signaling-components-in-adipose-derived-stromal-fibroblasts
#2
Wei Luo, John Sandy, Katie Trella, Daniel Gorski, Shuguang Gao, Jun Li, Sabrina Brounts, Jorge Galante, Anna Plaas
Equine degenerative suspensory ligament desmitis (DSLD) in Peruvian Paso horses typically presents at 7-15 years and is characterized by lameness, focal disorganization of collagen fibrils, and chondroid deposition in the body of the ligament. With the aim of developing a test for disease risk (that can be used to screen horses before breeding) we have quantified the expression of 76 TGFβ-signaling target genes in adipose-derived stromal fibroblasts (ADSCs) from six DSLD-affected and five unaffected Paso horses...
2016: PloS One
https://www.readbyqxmd.com/read/27893580/the-effects-of-adipose-derived-stem-cell-differentiated-adipocytes-on-skin-burn-wound-healing-in-rats
#3
Yu-Wen Chen, Tabita Timeea Scutaru, Nicolae Ghetu, Eugen Carasevici, Cristian Dumitru Lupascu, Dan Ferariu, Dragos Pieptu, Corneliu-George Coman, Mihai Danciu
Both adipose-derived stem cells (ADSCs) and fat grafting promote burn wound healing, but whether adipogen-derived cells using various inducers such as 3-isobutyl-1-methylxanthine (IBMX) and insulin affect wound healing is unknown. Herein, ADSC-differentiated adipogenic lineages were used in rat burn wounds to evaluate wound healing potential. ADSCs were cultivated using six different adipogenic differentiation conditions (IBMX ± insulin, IBMX for 5 days, high and low Dulbecco's modified Eagle's medium) and in vitro morphological changes and cell proliferations during adipogenic differentiation were recorded...
November 11, 2016: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/27893514/improved-bone-regeneration-with-multiporous-plga-scaffold-and-bmp-2-transduced-human-adipose-derived-stem-cells-by-cell-permeable-peptide
#4
Suhyun Park, Hyun-A Heo, Kwang-Bae Lee, Han-Goo Kim, Sung-Woon Pyo
OBJECTIVE: Currently, much work has focused on the engineering of bone using adipose-derived stem cells (ADSCs), which differentiate into osteogenic cells. This study was conducted to assess the bone-regenerating capacity of ADSCs with genetic modification. MATERIALS AND METHODS: ADSCs were cultured and transduced with recombinant adenovirus-expressing bone morphogenetic protein-2 (rAd/BMP-2). Two 5-mm full-thickness bone defects were created on the parietal bones of 24 rats...
November 23, 2016: Implant Dentistry
https://www.readbyqxmd.com/read/27887732/expressions-of-pathologic-markers-in-prp-based-chondrogenic-differentiation-of-human-adipose-derived-stem-cells
#5
Arezou Pakfar, Shiva Irani, Hana Hanaee-Ahvaz
BACKGROUND: Optimization of the differentiation medium through using autologous factors such as PRP is of great consideration, but due to the complex, variable and undefined composition of PRP on one hand and lack of control over the absolute regulatory mechanisms in in vitro conditions or disrupted and different mechanisms in diseased tissue microenvironments in in vivo conditions on the other hand, it is complicated and rather unpredictable to get the desired effects of PRP making it inevitable to monitor the possible pathologic or undesired differentiation pathways and therapeutic effects of PRP...
November 12, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/27878248/immunomodulatory-effects-of-ox40ig-gene-modified-adipose-tissue-derived-mesenchymal-stem-cells-on-rat-kidney-transplantation
#6
Tao Liu, Yue Zhang, Zhongyang Shen, Xunfeng Zou, Xiaobo Chen, Li Chen, Yuliang Wang
Recent studies have suggested that adipose tissue-derived mesenchymal stem cell (ADSC) therapy and OX40 costimulation blockade are two immunomodulatory strategies used to suppress the immune response to alloantigens. However, relatively little has been reported regarding the immunomodulatory potential of the abilityof these two strategies to synergize. Thus, in the present study, we aimed to investigate OX40-Ig fusion protein (OX40Ig) expression in ADSCs and to validate their more potent immunosuppressive activity in preventing renal allograft rejection...
November 21, 2016: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27819724/histone-h3k9-acetylation-influences-growth-characteristics-of-goat-adipose-derived-stem-cells-in-vitro
#7
X Wang, F X Zhang, Z M Wang, Q Wang, H F Wang, Y Ren, D P Tai, H Liang, D J Liu
Adipose-derived stem cells (ADSCs) show nearly unlimited potential in medical and animal science. Currently, understanding of the biological mechanisms regulating ADSC growth in vitro remains very limited. Histone acetylation, an epigenetic modification, plays a key role in maintaining stem cell properties. To further study its effect on ADSC growth characteristics in vitro, we treated goat ADSCs with the histone deacetylase inhibitors trichostatin A (TSA) and vorinostat (SAHA). This inhibited SIRT1 expression and increased histone H3K9 acetylation, leading to decreased cell viability, cell cycle arrest, and apoptosis...
November 3, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27804241/towards-reconstruction-of-epithelialized-cartilages-from-autologous-adipose-tissue-derived-stem-cells
#8
Sophie E P New, Amel Ibrahim, Leonardo Guasti, Eleonora Zucchelli, Martin Birchall, Neil W Bulstrode, Alexander M Seifalian, Patrizia Ferretti
Deformities of the upper airways, including those of the nose and throat, are typically corrected by reconstructive surgery. The use of autologous somatic stem cells for repair of defects could improve quality and outcomes of such operations. The present study explored the ability of paediatric adipose-derived stem cells (pADSCs), a readily available source of autologous stem cells, to generate a cartilage construct with a functional epithelium. Paediatric ADSCs seeded on the biodegradable nanocomposite polymer, polyhedral oligomeric silsesquioxane poly(ϵ-caprolactone-urea) urethane (POSS-PCL), proliferated and differentiated towards mesenchymal lineages...
November 1, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27803921/use-of-adipose-derived-stem-cells-to-support-topical-skin-adhesive-for-wound-closure-a-preliminary-report-from-animal-in-vivo-study
#9
Maciej Nowacki, Katarzyna Pietkun, Arkadiusz Jundziłł, Tomasz Kloskowski, Dariusz Grzanka, Joanna Skopinska-Wisniewska, Kinga Scibior, Maciej Gagat, Marta Pokrywczyńska, Alina Grzanka, Wojciech Zegarski, Rafał Czajkowski, Tomasz Drewa, Barbara Zegarska
The aim of this study was to determine the local and systemic effects of adipose-derived stem cells (ADSCs) as a component of topical skin adhesive in an animal artificial wound closure model. In presented study the cosmetic effects, histological analysis, mechanical properties, and cell migration have been assessed to evaluate the usefulness of ADSCs as supporting factor for octyl blend cyanoacrylate adhesive. The total of 40 rats were used and divided into six groups. In the Study Group, ADSCs were administered by multipoint injection of the six surrounding intrawound areas with additional freely leaving procedure of the cells between the skin flaps just before applying adhesive to close the wound...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27791278/conditioned-media-from-adipose-tissue-derived-mesenchymal-stem-cells-reverse-insulin-resistance-in-cellular-models
#10
Nitya Shree, Ramesh R Bhonde
The link between insulin resistance (IR) and type 2 diabetes has been recognized for a long time. Type 2 diabetes is often associated with basal hyperinsulinemia, reduced sensitivity to insulin, and disturbances in insulin release. There are evidences showing the reversal of IR by mesenchymal stem cells. However, the effect of conditioned media from adipose derived mesenchymal stem cells (ADSCs-CM) in reversal of IR has not been established. We established an insulin resistant model of 3T3L1 and C2C12 cells and treated with ADSCs-CM...
October 28, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27790299/effect-of-adipose-tissue-derived-stem-cell-injection-in-a-rat-model-of-urethral-fibrosis
#11
Premsant Sangkum, Faysal A Yafi, Hogyoung Kim, Mostafa Bouljihad, Manish Ranjan, Amrita Datta, Sree Harsha Mandava, Suresh C Sikka, Asim B Abdel-Mageed, Wayne J G Hellstrom
INTRODUCTION: We sought to evaluate the therapeutic effect of adi-pose tissue-derived stem cells (ADSCs) in a rat model of urethral fibrosis. METHODS: Eighteen (18) male Sprague-Dawley rats (300‒350 g) were divided into three groups: (1) sham (saline injection); (2) urethral fibrosis group (10 μg transforming growth factor beta 1 (TGF-β1) injection); and (3) ADSCs group (10 μg TGF-β1 injection plus 2 × 10(5) ADSCs). Rat ADSCs were harvested from rat inguinal fat pads...
May 2016: Canadian Urological Association Journal, Journal de L'Association des Urologues du Canada
https://www.readbyqxmd.com/read/27775060/adipose-tissue-hyperplasia-with-enhanced-adipocyte-derived-stem-cell-activity-in-tc1-c8orf4-deleted-mice
#12
Hayoung Jang, Minsung Kim, Soyoung Lee, Jungtae Kim, Dong-Cheol Woo, Kyung Won Kim, Kyuyoung Song, Inchul Lee
Adipose tissue hyperplasia with increased number of adipocytes is implicated in a protective rather than deleterious effect on obesity-associated metabolic disorder. It is poorly understood how the adipose tissue cellularity is regulated. Tc1 is a gene of vertebrates that regulates diverse downstream genes. Young Tc1-deleted mice fed on standard chow diet show expanded adipose tissue with smaller adipocytes in size compared to wild type controls, representing adipose tissue hyperplasia. Tc1(-/-) mice show enhanced glucose tolerance and reduced serum lipids...
October 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27774940/-wnt-%C3%AE-catenin-pathway-involved-in-the-regulation-of-rat-mesangial-cell-proliferation-by-adipose-derived-mesenchymal-stem-cells
#13
Zhi Li, Mengying Zhang, Xueqin Li, Jinming Lu, Liang Xu
Objective To investigate the effect of adipose-derived mesenchymal stem cells (ADSCs) on glomerular mesangial cell proliferation via Wnt/β-catenin pathway. Methods The rat glomerular mesangial cells (HBZY-1) were incubated in conditioned ADSC medium. Cell cycle was analyzed with flow cytometry; the proliferation rate of HBZY-1 and the expression levels of relative genes and proteins of Wnt signaling pathway were measured using RNA interference, quantitative real-time PCR and Western blotting, respectively...
November 2016: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/27770886/characterization-of-human-adipose-tissue-derived-stem-cells-in-vitro-culture-and-in-vivo-differentiation-in-a-temperature-sensitive-chitosan-%C3%AE-glycerophosphate-collagen-hybrid-hydrogel
#14
Kedong Song, Liying Li, Xinyu Yan, Wen Zhang, Yu Zhang, Yiwei Wang, Tianqing Liu
In this study, the interaction of human adipose tissue-derived stem cells (ADSCs) with chitosan/β-glycerophosphate/collagen (C/GP/Co) hybrid hydrogel was test, followed by investigating the capability of engineered adipose tissue formation using this ADSCs seeded hydrogel. The ADSCs were harvested and mixed with a C/GP/Co hydrogel followed by a gelation at 37°C and an in vitro culture. The results showed that the ADSCs within C/GP/Co hydrogels achieved a 30% of expansion over 7days in culture medium and encapsulated cell in C/GP/Co hydrogel demonstrated a characteristic morphology with high viability over 5days...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27765088/-effects-of-local-transplantation-of-autologous-adipose-derived-mesenchymal-stem-cells-on-the-formation-of-hyperplastic-scar-on-rabbit-ears
#15
L Chen, D L Wang, Z R Wei, B Wang, J P Qi, G F Sun
Objective: To investigate the effects of local transplantation of autologous adipose-derived mesenchymal stem cells (ADSCs) on the formation of hyperplastic scar on rabbit ears. Methods: ADSCs were isolated from inguinal fat of six New Zealand rabbits and then sub-cultured. ADSCs of the third passage of each rabbit were used in the following experiments. Six full-thickness skin defect wounds with diameter of 6 mm on the ventral surface of every rabbit ear were made. Wound healing and local-tissue proliferation were observed, and complete epithelization time of wounds and formation time of hyperplastic scar were recorded...
October 20, 2016: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/27761667/co2-laser-increases-the-regenerative-capacity-of-human-adipose-derived-stem-cells-by-a-mechanism-involving-the-redox-state-and-enhanced-secretion-of-pro-angiogenic-molecules
#16
Alina Constantin, Madalina Dumitrescu, Maria Cristina Mihai Corotchi, Dana Jianu, Maya Simionescu
CO2 laser has a beneficial effect on stem cells by mechanisms that are not clearly elucidated. We hypothesize that the effect of fractional CO2 laser on human adipose-derived stem cells (ADSC) could be due to changes in redox homeostasis and secretion of factors contributing to cellular proliferation and angiogenic potential. ADSC incubated in medium containing 0.5 or 10 % FBS were exposed to a single irradiation of a 10,600-nm fractional CO2 laser; non-irradiated ADSC were used as control. Viability/proliferation of ADSC was assessed by MTT assay; the intracellular reactive oxygen species (ROS) levels and the mitochondrial membrane potential (∆Ψm) were determined with DCFH-DA and JC-1 fluorescent probes, respectively...
October 20, 2016: Lasers in Medical Science
https://www.readbyqxmd.com/read/27760639/-study-on-the-survival-rate-of-random-flap-using-pre-injection-of-adscs
#17
B S Wang, H Y Yang, Y Han
Objective: To determine the effects of adipose-derived stem cells (ADSCs) on the survival rate of flaps by transplanting ADSCs into the experimental flaps pre-operatively. Methods: ADSCs were isolated from fresh human fat and cultured in vitro. Their morphological characters were observed, and flowcytometry and differentiation test and evaluation were conducted. A total of 40 BALB/c mice were divided into 5 groups randomly with each of 8 mice. 1 cm×4 cm random skin flap was designed on the mice back, ADSCs were injected in advance into subcutaneous tissues of 3 groups, the operation was performed on the 2(nd,) 5(th) and 7(th) day after the ADSCs injection, meanwhile skin flap operation was conducted in the immediate injection group and control group...
September 27, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/27736251/analysis-of-the-effect-of-locally-applied-bovine-collagen-sponge-and-adipose-derived-mesenchymal-stem-cells-on-seroma-development-in-rats-undergone-mastectomy-and-axillary-dissection
#18
Hasan Bakır, Erdal Uysal, Akif Hakan Kurt, Türkay Kirdak
PURPOSE: Seroma is the most frequently seen complication after the mastectomy and axillary dissection. The aim of this study was to analyze the effects of locally applied bovine collagen sponge and adipose-derived mesenchymal stem cells on seroma development in rats that undergone mastectomy and axillary dissection. MATERIALS AND METHODS: Wistar albino rats, were randomly divided into 3 groups (n = 10 per group). For the rats in Group 1, 1 ml 0.09% NaCl was implemented...
October 13, 2016: Journal of Investigative Surgery: the Official Journal of the Academy of Surgical Research
https://www.readbyqxmd.com/read/27735181/the-enhanced-osteogenesis-of-adscs-by-the-synergistic-effect-of-aligned-fibers-containing-collagen-i
#19
HanBang Chen, YunZhu Qian, Yang Xia, Gang Chen, Yun Dai, Na Li, FeiMin Zhang, Ning Gu
The topographical features and material composition of nanoscaffolds have a powerful influence on cell behaviors such as proliferation and differentiation. Here, scaffolds consisting of aligned nanofibers with incorporated bioactive collagen I were tested for their ability to enhance osteogenesis in vitro. Rat adipose-derived mesenchymal stem cells (ADSCs) were seeded on the scaffolds and their morphology, proliferation and osteogenic differentiation were examined. Aligned scaffolds with collagen I showed the best osteogenic properties...
October 13, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27733786/identification-and-characterization-of-pig-adipose-derived-progenitor-cells
#20
Shuang Zhang, Chunyu Bai, Dong Zheng, Yuhua Gao, Yanan Fan, Lu Li, Weijun Guan, Yuehui Ma
Adipose-derived stem cells (ADSCs) are multipotent, and can be differentiated into many cell types in vitro. In this study, tissues from pigs were chosen to identify and characterize ADSCs. Primary ADSCs were sub-cultured to passage 28. The surface markers of ADSCs: CD29, CD71, CD73, CD90, and CD166 were detected by reverse-transcription polymerase chain reaction assays and the markers CD29, CD44, CD105, and vimentin were detected by immunofluorescence. Growth curves and the capacity of clone-forming were performed to test the proliferation of ADSCs...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
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