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https://www.readbyqxmd.com/read/29770615/isotropic-differential-phase-contrast-microscopy-for-quantitative-phase-bio-imaging
#1
Hsi-Hsun Chen, Yu-Zi Lin, Yuan Luo
Quantitative phase imaging has been investigated to retrieve optical phase information of an object, and applied to biological microscopy and related medical studies. In recent examples, differential phase contrast (DPC) microscopy can recover phase image of thin sample under multi-axis intensity measurements in wide field scheme. Unlike conventional DPC, based on theoretical approach under partially coherent condition, we propose a new method to achieve isotropic differential phase contrast (iDPC) with high accuracy and stability for phase recovery in simple and high-speed fashion...
May 16, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/29764508/adipose-derived-stem-cells-for-treatment-of-chronic-ulcers-current-status
#2
REVIEW
Jens Selch Holm, Navid Mohamadpour Toyserkani, Jens Ahm Sorensen
Chronic ulcers remain a difficult challenge in healthcare systems. While treatment options are limited, stem cells may be a novel alternative. Adipose-derived stem cells (ADSC) have become increasingly popular compared with bone marrow-derived stem cells as they are far easier to harvest. To summarize the current status of treating chronic ulcers with ADSC, this systematic review includes all clinical trials on the subject from PubMed and EmBase, as well as all registered clinical trials on ClinicalTrials.Gov...
May 15, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29763507/effect-of-titanium-surfaces-on-the-osteogenic-differentiation-of-human-adipose-derived-stem-cells
#3
Diogo Godoy Zanicotti, Warwick John Duncan, Gregory John Seymour, Dawn Elizabeth Coates
PURPOSE: To investigate whether human adipose-derived stem cells will form a functional bone-like matrix on titanium substrates. The behavior of human adipose-derived stem cells was examined when grown in either serum-free, xeno-free stem cell growth medium or osteogenic differentiation medium and cultured on either machined titanium (MTi) or on roughened alumina-blasted titanium (ABTi) discs. MATERIALS AND METHODS: Cellular proliferation, extracellular mineralized matrix production, osteogenic-related protein production (RUNX2 and osteocalcin), and gene expression for pluripotency and self-renewal (TERT and OCT4) and osteogenic-related (MSX2, RUNX2, and BGLAP) genes were performed...
May 2018: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/29756519/adipose-derived-stem-cells-stimulated-with-n-butylidenephthalide-exhibit-therapeutic-effects-in-a-mouse-model-of-parkinson-s-disease
#4
Kang Chi, Ru-Huei Fu, Yu-Chuen Huang, Shih-Yin Chen, Ching-Ju Hsu, Shinn-Zong Lin, Chi-Tang Tu, Li-Hsun Chang, Ping-An Wu, Shih-Ping Liu
Parkinson's disease (PD) causes motor dysfunction and dopaminergic cell death. Drug treatments can effectively reduce symptoms but often cause unwanted side effects. Stem cell therapies using cell replacement or indirect beneficial secretomes have recently emerged as potential therapeutic strategies. Although various types of stem cells have been proposed as possible candidates, adipose-derived stem cells (ADSCs) are easily obtainable, more abundant, less ethically disputed, and able to differentiate into multiple cell lineages...
January 1, 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29752466/mir-5591-5p-regulates-the-effect-of-adscs-in-repairing-diabetic-wound-via-targeting-ages-ager-jnk-signaling-axis
#5
Qiang Li, Sizhan Xia, Yating Yin, Yanping Guo, Feifei Chen, Peisheng Jin
Advanced glycation end products/advanced glycation end products receptor (AGEs/AGER) interaction triggers reactive oxygen species (ROS) generation and activates downstream signal pathways and induces apoptosis in endothelial progenitor cells. A number of studies have revealed the involvement of microRNAs (miRNAs) in regulating intracellular ROS production and apoptosis. However, few studies explore the role of miRNAs in regulating the effect of adipose tissue-derived stem cells (ADSCs) in repairing diabetic wound and the associated cellular mechanisms remain unclear...
May 11, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29751779/essential-role-of-atp6ap2-enrichment-in-caveolae-lipid-raft-microdomains-for-the-induction-of-neuronal-differentiation-of-stem-cells
#6
Nehman Makdissy, Katia Haddad, Jeanne D'arc AlBacha, Diana Chaker, Bassel Ismail, Albert Azar, Ghada Oreibi, David Ayoub, Ibrahim Achkar, Didier Quilliot, Ziad Fajloun
BACKGROUND: The subcellular distribution of prorenin receptor and adaptor protein ATP6AP2 may affect neurogenesis. In this study, we hypothesized that ATP6AP2 expression and subcellular relocalization from caveolae/lipid raft microdomains (CLR-Ms) to intracellular sites may correlate with neuronal differentiation (Neu-Dif) of adipose-derived mesenchymal stem cells (ADSCs). METHODS: Human ADSCs isolated from 24 healthy donors and 24 patients with neurological disorders (ND) were cultured and induced for Neu-Dif...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29745446/effects-of-vegf-ang-1-pla-nano-sustained-release-microspheres-on-proliferation-and-differentiation-of-adscs
#7
Yucang He, Zihao Li, Zhuojie Chen, Xiaofang Yu, Ziwan Ji, Jingping Wang, Yao Qian, Liqun Li
The improvement of fat graft viability might depend on the presence of multipotent resident adipose derived stem cells (ADSCs) which is the important component of stromal vascular fraction (SVF). Vascular endothelial growth factor (VEGF) and angiogenin-1 (Ang-1) are responsible for neovascularization. However, their half-life is too short to produce a biological effect. We thus investigated whether VEGF-ANG-1-polylactic acid (PLA) microspheres could enhance the angiogenic properties of ADSCs. PLA microspheres containing VEGF and ANG-1 were prepared by in vitro ultrasonic emulsification and characterized according to their encapsulation efficiency (EE), drug-loading rate (DL), particle size and drug release...
May 10, 2018: Cell Biology International
https://www.readbyqxmd.com/read/29744414/human-adipose-derived-stem-cells-are-superior-to-human-osteoblasts-hob-in-bone-tissue-engineering-on-a-collagen-fibroin-elr-blend
#8
Esen Sayin, Rosti Hama Rashid, José Carlos Rodríguez-Cabello, Ahmed Elsheikh, Erkan Türker Baran, Vasif Hasirci
The ultrastructure of the bone provides a unique mechanical strength against compressive, torsional and tensional stresses. An elastin-like recombinamer (ELR) with a nucleation sequence for hydroxyapatite was incorporated into films prepared from a collagen - silk fibroin blend carrying microchannel patterns to stimulate anisotropic osteogenesis. SEM and fluorescence microscopy showed the alignment of adipose-derived stem cells (ADSCs) and the human osteoblasts (HOBs) on the ridges and in the grooves of microchannel patterned collagen-fibroin-ELR blend films...
June 2017: Bioactive Materials
https://www.readbyqxmd.com/read/29737401/transplantation-of-adipose-tissue-derived-stem-cells-ameliorates-autoimmune-pathogenesis-in-mrl-lpr-mice-modulation-of-the-balance-between-th17-and-treg
#9
W Zhang, Y-L Feng, C-Y Pang, F-A Lu, Y-F Wang
BACKGROUND: Mesenchymal stem cells (MSCs) are multipotent cells characterized by immunomodulatory properties and are therefore considered a promising tool for the treatment of autoimmune diseases. In this study, we aimed to investigate whether transplantation of adipose tissue-derived stem cells (ADSCs) affects the autoimmune pathogenesis in MRL/lpr mice. METHODS: Fifteen 12-week-old MRL/lpr mice were randomly divided into three groups: ADSC, cyclophosphamide (CTX), and control groups, with five mice in each group...
May 8, 2018: Zeitschrift Für Rheumatologie
https://www.readbyqxmd.com/read/29731771/undifferentiated-adipose-tissue-stem-cell-transplantation-promotes-hepatic-regeneration-ameliorates-histopathologic-damage-of-the-liver-and-upregulates-the-expression-of-liver-regeneration-and-liver-specific-genes-in-a-rat-model-of-partial-hepatectomy
#10
Konstantinos G Apostolou, Ioannis G Papanikolaou, Charalampos Katselis, Themistoklis Feretis, Dimitrios Kletsas, Manousos M Konstadoulakis, Maria Lymperi, Angelica A Saetta, Spiros Tsikalakis, George Agrogiannis, Efstratios Patsouris, George C Zografos, Apostolos E Papalois
Objective: Adipose tissue stem cells (ADSCs) present a promising therapeutic method to alleviate liver failure (LF). The purpose of this prospective study was to evaluate the efficacy of undifferentiated ADSC transplantation on liver regeneration and on the expression of liver regeneration- and liver-specific genes, following 60% partial hepatectomy (PHx). Methods: Sixty female rats were subjected to PHx and were transplanted with 106 or 2 × 106 ADSCs, either into the portal vein (PV) or into the hepatic parenchyma...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29725380/tgf-%C3%AE-3-reduces-apoptosis-in-ischemia-induced-adipose-derived-stem-cells-by-enhancing-dna-repair
#11
Fan Wu, Haiwen Ye, Junfeng Lin, Yaodong Xu, Zhuasong Zhang, Hao Xiong, Maojin Laing, Yiqing Zhen, Suijun Chen
Adipose-derived stem cells (ADSCs) possess good proliferative and differentiative abilities, making then a promising candidate for the treatment of cartilage defects. However, local ischemia often causes apoptosis in ADSCs. Transforming growth factor-β3 (TGF-β3) is often used as a chondrogenic differentiation cytokine whose function in apoptosis is unclear. The aim of the present study was to investigate the role of TGF-β3 in ischemia-induced ADSC apoptosis. In the present study, the phenotypes and multipotent differentiation properties of human ADSCs at passage 3 were analyzed using flow cytometry and cytochemical staining...
May 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29722950/-adipose-derived-stem-cells-for-the-treatment-of-penile-erectile-dysfunction-an-update
#12
REVIEW
Yi Wang, Ya-Min Wang, Chen Chen, Yi-Chun Wang, Ning-Hong Song
Adipose-derived stem cells (ADSCs) are pluripotent stem cells isolated from the adipose tissue and have the potential for self-renewal and multi-directional differentiation into neurogenic cells, smooth muscle cells, endothelial cells, and so on. Erectile dysfunction (ED) is a common male sexual dysfunction that has a negative impact on the lives of the patients and their partners. Current treatments of ED include surgery and medication, with oral 5-phosphodiesterase inhibitors as the first-line drugs. However, a small number of the patients are not sensitive to these therapies and cannot be improved or cured pathologically...
June 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29722292/angiogenic-potential-of-spheroids-from-umbilical-cord-and-adipose-derived-multipotent-mesenchymal-stromal-cells-within-fibrin-gel
#13
Anastasiya Gorkun, Anastasia Shpichka, Irina Zurina, Anastasia V Koroleva, Nastasia V Kosheleva, Denis Aleksandrovich Nikishin, Denis Butnaru, Peter S Timashev, Vadim S Repin, Irina Saburina
One of the essential goals in regenerative medicine is microvascularization which enables an effective blood supply within de novo constructed tissues and organs. In our study, we used two common multipotent mesenchymal stromal cell sources (subcutaneous adipose tissue and Wharton's jelly of the umbilical cord) where is a subpopulation of endothelial precursors. In the medium supplemented with VEGF, the 3D cultures of UC MMSCs and ADSCs promoted the endothelial cell differentiation. To evaluate their ability to form a capillary-like network, we encapsulated spheroids within non-modified and PEGylated fibrin hydrogels...
May 3, 2018: Biomedical Materials
https://www.readbyqxmd.com/read/29719691/injectable-hyperbranched-poly-%C3%AE-amino-ester-hydrogels-with-on-demand-degradation-profiles-to-match-wound-healing-processes
#14
Qian Xu, Linru Guo, Sigen A, Yongsheng Gao, Dezhong Zhou, Udo Greiser, Jack Creagh-Flynn, Hong Zhang, Yixiao Dong, Lara Cutlar, Fagang Wang, Wenguang Liu, Wei Wang, Wenxin Wang
Adjusting biomaterial degradation profiles to match tissue regeneration is a challenging issue. Herein, biodegradable hyperbranched poly(β-amino ester)s (HP-PBAEs) were designed and synthesized via "A2 + B4" Michael addition polymerization, and displayed fast gelation with thiolated hyaluronic acid (HA-SH) via a "click" thiol-ene reaction. HP-PBAE/HA-SH hydrogels showed tunable degradation profiles both in vitro and in vivo using diamines with different alkyl chain lengths and poly(ethylene glycol) diacrylates with varied PEG spacers...
February 28, 2018: Chemical Science
https://www.readbyqxmd.com/read/29713784/limited-evidence-for-adipose-derived-stem-cell-therapy-on-the-treatment-of-osteoarthritis
#15
REVIEW
Eoghan T Hurley, Youichi Yasui, Arianna L Gianakos, Dexter Seow, Yoshiharu Shimozono, Gino M M J Kerkhoffs, John G Kennedy
PURPOSE: The purpose of this systematic review is to evaluate the effects of adipose derived mesenchymal stem cells (ADSCs) in the treatment of osteoarthritis (OA) in the clinical setting. METHODS: A literature search was performed in the MEDLINE, EMBASE, and The Cochrane Library Database up to January 2017 for inclusion and exclusion criteria. Criteria for inclusion were clinical studies demonstrating the effects of ADSCs on OA, and written in English. The following variables were analyzed: donor site, volume of adipose tissue, preparation of ADSCs, clinical outcomes, and complication rate...
April 30, 2018: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/29705934/exosomes-secreted-by-adipose-derived-stem-cells-contribute-to-angiogenesis-of-brain-microvascular-endothelial-cells-following-oxygen-glucose-deprivation-in-vitro-through-microrna-181b-trpm7-axis
#16
Yujia Yang, Yue Cai, Yuan Zhang, Juan Liu, Zhiqiang Xu
Adipose-derived stem cells (ADSCs) have been demonstrated to promote cerebral vascular remodeling processes after stroke. However, the exact molecular mechanism by which ADSCs exert protective roles in ischemic stroke is still poorly understood. In this study, we identified the role of exosomal microRNA-181b-5p (181b-Exos) in regulating post-stroke angiogenesis. The results of migration assay and capillary network formation assay showed that exosomes secreted by ADSCs (ADSCs-Exos) promoted the mobility and angiogenesis of brain microvascular endothelial cells (BMECs) after oxygen-glucose deprivation (OGD)...
April 29, 2018: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/29704575/the-glutamate-clearance-function-of-adipose-stromal-cells-derived-astrocytes
#17
Zanzan Cheng, Ya Ou, Lili Zhang, Pingshu Zhang, Xiaodong Yuan, Wei Peng, Shujuan Wang, Xuhong Zhu, Liping Zhang, Yan Meng
ADSCs-derived astrocytes qualify the morphology, ultrastructure and membrane electrical potential, which are all unique to astrocytes. But whether they have the glutamate clearance function like mature astrocytes is under exploration. ADSCs were extracted, cultured and induced into astrocytes for 48 h, 7d, 14d and 21d in vitro. Inverted phase contrast microscope was used to observe the morphology of the cells in each group. Immunocytochemistry assay, immunofluorescence assay and Western blotting were used to detect the expression of GFAP, EAAT2 and GS of the cells in each group...
April 25, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29699385/local-mesenchymal-stem-cell-therapy-in-experimentally-induced-colitis-in-the-rat
#18
Fernando de la Portilla, Yaiza Yuste, Sheila Pereira, Carolina Olano, Maria Victoria Maestre, Francisco Javier Padillo
Background: Multipotent mesenchymal stem cells (MSCs) have been used in inflammatory bowel diseases because of their immunomodulatory and regenerative properties. We investigated their local use in an experimental model of colitis in the rat. Materials and Methods: Colitis was induced into 20 Wistar rats with local TNBS instillation. Allogeneic stem cells were derived from rat adipose tissue and labeled with PKH2 linker dye with creation of a control and a second group treated by a local injection into the rectal wall of 2×10⁶ allogeneic adipose tissue-derived stem cells (ADSCs)...
April 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/29688701/nanocomposite-porous-microcarriers-based-on-strontium-substituted-ha-g-poly-%C3%AE-benzyl-l-glutamate-for-bone-tissue-engineering
#19
Shifeng Yan, Pengfei Xia, Shenghua Xu, Kunxi Zhang, Guifei Li, Lei Cui, Jingbo Yin
Porous microcarriers have aroused increasing attention recently, which can create a protected environment for sufficient cell seeding density, facilitate oxygen and nutrient transfer, and well support the cell attachment and growth. In this study, porous microcarriers fabricated from the strontium-substituted hydroxyapatite-graft-poly(γ-benzyl-L-glutamate) (Sr10-HA-g-PBLG) hybrid nanocomposite were developed. The surface grating of PBLG, the micromorphology and element distribution, mechanical strength, in vitro degradation and Sr2+ ion release of the obtained Sr10-HA-g-PBLG porous microcarriers were investigated, respectively...
April 24, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29687384/adipose-derived-stromal-vascular-fraction-cells-and-platelet-rich-plasma-basic-and-clinical-implications-for-tissue-engineering-therapies-in-regenerative-surgery
#20
Pietro Gentile, Valerio Cervelli
Cell-based therapy and regenerative medicine offer a paradigm shift in regard to various diseases causing loss of substance or volume and tissue or organ damage. Recently, many authors have focused their attention on mesenchymal stem cells for their capacity to differentiate into many cell lineages. The most widely studied types are bone marrow mesenchymal stem cells and adipose derived stem cells (ADSCs), which display similar results. Based on the literature, we believe that the ADSCs offer advantages because of lower morbidity during the harvesting procedure...
2018: Methods in Molecular Biology
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