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https://www.readbyqxmd.com/read/28437187/synergy-between-choroid-plexus-epithelial-cells-conditioned-medium-and-knockout-serum-replacement-converts-human-adipose-derived-stem-cells-to-dopamine-secreting-neurons
#1
Mahdi Eskandarian Boroujeni, Mossa Gardaneh, Mehrnoosh Hasan Shahriari, Abbas Aliaghaei, Sanaz Hasani
Human adipose-derived stem cells (hADSCs) have great capacity to differentiate into mesodermal origins as well as non-mesodermal lineages including neural cells. This valuable feature paves the way for the therapeutic application of hADSCs for neurodegenerative maladies such as Parkinson's disease (PD). We tested the capacity of Choroid Plexus epithelial cells-conditioned medium (CPEC-CM) alone or cocktailed with knock-out serum (KS) to induce dopaminergic differentiation of hADSCs. To this end, hADSCs from lipoaspirate were phenotypically characterized and shown to maintain mesodermal multipotency so selected media easily differentiated them into osteoblasts, chondrocytes and adipocytes...
March 2, 2017: Rejuvenation Research
https://www.readbyqxmd.com/read/28429362/wnt11-overexpression-promote-adipose-derived-stem-cells-differentiating-to-the-nucleus-pulposus-like-phenotype
#2
H-T Chen, A-B Huang, Y-L He, J Bian, H-J Li
OBJECTIVE: Our present study aimed to evaluate the effects of Wnt11 overexpression on the adipose-derived stem (ADSCs) cells differentiation to the nucleus pulposus (NP) cells and its function in the ADSCs cells growth, proliferation and induction of the NP cells markers. MATERIALS AND METHODS: The cell growth was detected using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) (MTT) assay and the cell cycle was assessed by the flow cytometry...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28423603/adipose-derived-mesenchymal-stem-cells-promote-osteosarcoma-proliferation-and-metastasis-by-activating-the-stat3-pathway
#3
Yan Wang, Yijing Chu, Bin Yue, Xuexiao Ma, Guoqing Zhang, Hongfei Xiang, Yong Liu, Tianrui Wang, Xiaolin Wu, Bohua Chen
Osteosarcoma is the most common primary bone malignancy in children and young adults, but the role of adipose-derived mesenchymal stem cells (ADSCs) in the rapid progression of osteosarcoma is still unclear. Here, we found that ADSCs promoted tumour growth and invasion by increasing matrix metalloproteinase 2/9 (MMP2/9) expression in tumour cells. The persistent activation of signal transducer and activator of transcription 3 (STAT3) has been shown to directly promote tumour growth by mediating a wide spectrum of cellular responses, and STAT3 activation was detected in osteosarcoma cells co-cultured with ADSCs or treated with ADSC-conditioned medium...
April 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28419760/bone-marrow-derived-stem-stromal-cells-and-adipose-tissue-derived-stem-stromal-cells-their-comparative-efficacies-and-synergistic-effects
#4
REVIEW
Gun-Il Im
Mesenchymal stem cells (MSCs) are heterogeneous cell populations that serve as reserves for tissue regeneration in the presence of disease or injury. Although MSCs are found in various tissues, bone marrow-derived stem/stromal cells (BMSCs) and adipose tissue-derived stem/stromal cells (ADSCs) have been most thoroughly investigated. Furthermore, ADSCs have recently emerged as an attractive source of MSCs due to their abundance and availability. BMSCs and ADSCs demonstrate similar morphological characteristics, but their in vitro characteristics and differentiation abilities appear to differ...
April 17, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28419435/osteogenic-differentiation-potential-of-mesenchymal-stem-cells-cultured-on-nanofibrous-scaffold-improved-in-the-presence-of-pulsed-electromagnetic-field
#5
Monireh Arjmand, Abdolreza Ardeshirylajimi, Hossein Maghsoudi, Esmaeel Azadian
Nowadays, tissue engineering by using stem cells in combination with scaffolds and bioactive molecules has made significant contributions to the regeneration of damaged bone tissues. Since the usage of bioactive molecules including, growth factors to induce differentiation is safety limited in clinical applications, and it has also been previously observed that extremely low frequency pulsed electromagnetic fields (PEMF) can be effective in the enhancement of proliferation rate and osteogenic differentiation of stem cells, the aim of this study was investigating the osteoinductive potential of PEMF in combination with Poly(caprolactone) (PCL) nanofibrous scaffold...
April 17, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28419155/culture-expanded-allogenic-adipose-tissue-derived-stem-cells-attenuate-cartilage-degeneration-in-an-experimental-rat-osteoarthritis-model
#6
Li Mei, Bojiang Shen, Peixue Ling, Shaoying Liu, Jiajun Xue, Fuyan Liu, Huarong Shao, Jianying Chen, Aibin Ma, Xia Liu
Mesenchymal stem cell (MSC)-based cell therapy is a promising avenue for osteoarthritis (OA) treatment. In the present study, we evaluated the efficacy of intra-articular injections of culture-expanded allogenic adipose tissue-derived stem cells (ADSCs) for the treatment of anterior cruciate ligament transection (ACLT) induced rat OA model. The paracrine effects of major histocompatibility complex (MHC)-unmatched ADSCs on chondrocytes were investigated in vitro. Rats were divided into an OA group that underwent ACLT surgery and a sham-operated group that did not undergo ACLT surgery...
2017: PloS One
https://www.readbyqxmd.com/read/28417342/adipose-tissue-derived-stem-cell-therapy-for-erectile-dysfunction-in-rats-a-systematic-review-and-meta-analysis
#7
REVIEW
Quan-Liang Hou, Meng-Ying Ge, Cheng-da Zhang, Dan-Dan Tian, Lian-Ke Wang, Hui-Zi Tian, Wen-Hua Wang, Wei-Dong Zhang
OBJECTIVE: We aimed to systematically assess the effect of adipose tissue-derived stem cell (ADSC) therapy and its influential factors on the treatment of erectile dysfunction (ED) in rats. METHODS: Two authors independently searched for published studies through PubMed and EMBASE from study inception until August 31, 2016. A meta-analysis was used to combine the effect estimate from the published studies. A subgroup analysis was performed to identify the effect of some influential factors...
April 17, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28415420/in-vitro-cell-adhesion-and-proliferation-of-adipose-derived-stem-cell-on-hydroxyapatite-composite-surfaces
#8
Praneetha Pulyala, Akshay Singh, Marcela Ferreira Dias-Netipanyj, Sheron Compos Cogo, Luciane S Santos, Paulo Soares, Vasanth Gopal, V Suganthan, Geetha Manivasagam, Ketul C Popat
The goal of this work was to enhance the mechanical strength and fracture toughness of brittle hydroxyapatite (HAP) by reinforcing it with nanocomposites such as graphene oxide (GO), carbon nanotubes (CNT) and Titania. The goal was also to evaluate the cytotoxicity and the cellular adhesion/proliferation of these composites. The composites were characterized for their crystallinity, functionality, morphology and mechanical properties. Altering the composition by adding 1wt% GO and CNT significantly altered the wettability, hardness and roughness...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28409921/mesenchymal-stem-cell-seeded-regenerated-silk-fibroin-complex-matrices-for-liver-regeneration-in-an-animal-model-of-acute-liver-failure
#9
Lijuan Xu, Shufang Wang, Xiang Sui, Yu Wang, Yinan Su, Li Huang, Yunwei Zhang, Zheng Chen, Qianqian Chen, Haitao Du, Yaopeng Zhang, Li Yan
The main limitation of liver transplantation as a treatment for end-stage liver disease or acute liver failure is the scarcity of liver organ donors. To develop an alternative therapy for acute liver failure, mesenchymal stem cell (MSC)-seeded regenerated silk fibroin (RSF) matrices were evaluated in vitro and in vivo. Adipose-derived mesenchymal stem cells (ADSCs) and bone marrow-derived mesenchymal stem cells (BMSCs) were planted and grown on RSF scaffolds to form a scaffold complex. The RSF-MSC scaffold complex (the experimental group) and neat RSF scaffolds (the control group) were then placed onto the liver surface of mice induced by CCl4 and detected after 5, 7, 14, 28, and 60 days...
April 24, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28396222/activin-b-regulates-adipose-derived-mesenchymal-stem-cells-to-promote-skin-wound-healing-via-activation-of-the-mapk-signaling-pathway
#10
Lei Zhang, Pengcheng Xu, Xueer Wang, Min Zhang, Yuan Yan, Yinghua Chen, Lu Zhang, Lin Zhang
Adipose-derived stem cells (ADSCs) are multipotent stromal cells that can differentiate into a variety of cell types, including skin cells, and they can provide an abundant source of cells for skin tissue engineering and skin wound healing. The purpose of this study is to explore the therapeutic effects of activin B in combination with ADSCs and the possible signaling mechanism. In this study, we found that activin B was able to promote ADSC migration by inducing actin stress fiber formation in vitro. In vivo, activin B in combination with ADSCs was capable of enhancing α-SMA expression and wound closure...
April 8, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28382720/exosomes-derived-from-mir-181-5p-modified-adipose-derived-mesenchymal-stem-cells-prevent-liver-fibrosis-via-autophagy-activation
#11
Ying Qu, Qidi Zhang, Xiaobo Cai, Fei Li, Zhenzeng Ma, Mingyi Xu, Lungen Lu
Proliferating hepatic stellate cells (HSCs) respond to liver damage by secreting collagens that form fibrous scar tissue, which can lead to cirrhosis if in appropriately regulated. Advancement of microRNA (miRNA) hepatic therapies has been hampered by difficulties in delivering miRNA to damaged tissue. However, exosomes secreted by adipose-derived mesenchymal stem cells (ADSCs) can be exploited to deliver miRNAs to HSCs. ADSCs were engineered to overexpress miRNA-181-5p (miR-181-5p-ADSCs) to selectively home exosomes to mouse hepatic stellate (HST-T6) cells or a CCl4-induced liver fibrosis murine model and compared with non-targeting control Caenorhabditis elegans miR-67 (cel-miR-67)-ADSCs...
April 6, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28382066/adipose-tissue-derived-stem-cells-for-myocardial-regeneration
#12
REVIEW
Hyung Joon Joo, Jong-Ho Kim, Soon Jun Hong
Over the past decade, stem cell therapy has been extensively studied for clinical application for heart diseases. Among various stem cells, adipose tissue-derived stem cell (ADSC) is still an attractive stem cell resource due to its abundance and easy accessibility. In vitro studies showed the multipotent differentiation potentials of ADSC, even differentiation into cardiomyocytes. Many pre-clinical animal studies have also demonstrated promising therapeutic results of ADSC. Furthermore, there were several clinical trials showing the positive results in acute myocardial infarction using ADSC...
March 2017: Korean Circulation Journal
https://www.readbyqxmd.com/read/28380641/-development-of-an-innovative-cell-isolation-method-for-the-investigation-of-breast-cancer-pathogenesis-and-angiogenesis-for-experimental-in-vitro-and-in-vivo-assays
#13
Annika Weigand, Kereshmeh Tasbihi, Pamela L Strissel, Reiner Strick, Raymund E Horch, Anja M Boos
Breast cancer is the world's most common cancer among women. Autologous lipotransfer is increasingly used for breast reconstruction following surgical removal of the tumour. In cell-assisted lipotransfer, the transplant is enriched with stem cells from adipose tissue (ADSC). Despite positive clinical results, there are some concerns regarding oncological safety due to transplanted stem cells. To date there are only a few breast cancer studies using primary cells from the same patient to enable further investigation into the complexity of cell-cell interactions in breast cancer in an experimental setting...
April 5, 2017: Handchirurgie, Mikrochirurgie, Plastische Chirurgie
https://www.readbyqxmd.com/read/28376257/the-stiffness-of-a-crosslinked-hyaluronan-hydrogel-affects-its-chondro-induction-activity-on-hadscs
#14
Benjamin Teong, Shun-Cheng Wu, Chien-Mei Chang, Jhen-Wei Chen, Hui-Ting Chen, Chung-Hwan Chen, Je-Ken Chang, Mei-Ling Ho
Matrix stiffness plays an important role in stem cell differentiation. This study reports the synthesis of methacrylated hyaluronan (MeHA) with different degrees of methacrylation, ranging from 15 to 140% per disaccharide unit, which corresponds to a matrix stiffness ranging from 1.5 to 8 KPa. The swelling ratio was inversely proportional to the matrix stiffness, but the water content remained constant at >97% of the hydrogel mass. A fibril-like surface morphology and larger pore size were observed in lyophilized MeHA hydrogel with a lower stiffness...
April 4, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28364139/exercise-affects-biological-characteristics-of-mesenchymal-stromal-cells-derived-from-bone-marrow-and-adipose-tissue
#15
Sheng-Yao Liu, Yong-Bin He, Song-Yun Deng, Wen-Ting Zhu, Shao-Yong Xu, Guo-Xin Ni
Both bone marrow mesenchymal stromal cells (BMSCs) and adipose-derived mesenchymal stromal cells (ADSCs) are good sources for tissue engineering. To maximize therapeutic efficacy of MSCs, an appropriate source of MSCs should be selected according to their own inherent characteristics for future clinical application. Hence, this study was conducted to compare proliferative, differential and antiapoptosis abilities of both MSCs derived from exercised and sedentary rats under normal and hypoxia/serum deprivation conditions (H/SD)...
March 31, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28360526/human-omental-adipose-derived-mesenchymal-stem-cell-conditioned-medium-alters-the-proteomic-profile-of-epithelial-ovarian-cancer-cell-lines-in-vitro
#16
Yanling Zhang, Weihong Dong, Junjie Wang, Jing Cai, Zehua Wang
Mesenchymal stem cells (MSCs) have been reported to participate in the formation of supportive tumor stroma. The abilities of proliferation and invasion of human epithelial ovarian cancer (EOC) cells were significantly enhanced when indirectly cocultured with human omental adipose-derived MSCs (O-ADSCs) in vitro. However, the underlying mechanisms remain poorly understood. In this study, EOC cells were cultured with conditioned medium (CM) from O-ADSCs (O-ADSC), and the effect of O-ADSC CM on the proteomic profile of EOC cells was assessed by two-dimensional gel electrophoresis (2-DE), followed by liquid chromatography and tandem mass spectrometry...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28350842/combination-of-mesenchymal-stem-cell-injection-with-icariin-for-the-treatment-of-diabetes-associated-erectile-dysfunction
#17
Xiyou Wang, Chuanhai Liu, Yong Xu, Ping Chen, Yue Shen, Yansheng Xu, Yubo Zhao, Weihao Chen, Xinyu Zhang, Yun Ouyang, Yi Wang, Changliang Xie, Maojun Zhou, Cuilong Liu
The present study was aimed to examine whether icariin, a traditional Chinese medicine, could improve therapeutic effects of adipose derived mesenchymal stem cells (ADSCs) for diabetes-associated erectile dysfunction (DMED). DMED were induced in rats by intraperitoneal injection of streptozotocin and confirmed by erectile function measurement. Then, rats of diabetic ED were randomly divided to receive the treatment of saline, ADSCs, icariin or ADSCs combined with icariin respectively. Compared with the treatment by ADSCs or icariin alone, intracavernosum injection of ADSCs combined with the following daily gastric gavage of icariin significantly augmented the value of ICP and ICP/MAP (p<0...
2017: PloS One
https://www.readbyqxmd.com/read/28347699/effect-of-adipose-derived-stem-cells-on-acellular-dermal-matrix-engraftment-in-a-rabbit-model-of-breast-reconstruction
#18
Ung Sik Jin, Ki Yong Hong, Young-Il Hwang
Acellular dermal matrix (ADM) is frequently used in implant-based breast reconstruction. Although there are several advantages, ADM implantation also increases the risk of certain complications. Recently, ADM seeded with adipose-derived stem cells (ADSCs) were shown to induce angiogenesis and improve wound healing. This study aimed to investigate the effects of ADSCs on ADM engraftment in a rabbit model of implant-based breast reconstruction. Silicone implants were inserted to submuscular pocket of 16 female New Zealand rabbits using ADM with or without seeding of fluorescent PKH26-labelled rabbit ADSCs...
February 28, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/28343378/adipose-derived-stem-cells-suppress-inflammation-induced-by-il-1%C3%AE-through-down-regulation-of-p2x7r-mediated-by-mir-373-in-chondrocytes-of-osteoarthritis
#19
Rilong Jin, Miaoda Shen, Liedao Yu, Xuanwei Wang, Xiangjin Lin
Adipose-derived stem cells (ADSCs) were previously considered to have an anti-inflammatory effect, and Interleukin-1β (IL-1β) was found to be a pro-inflammatory factor in chondrocytes, but the mechanism underlying ADSCs and IL-1β is unclear. In this study, we investigate whether P2X7 receptor (P2X7R) signalling, regulated by microRNA 373 (miR-373), was involved in the ADSCs and IL-1β mediated inflammation in osteoarthritis (OA). Chondrocytes were collected from 20 OA patients and 20 control participants, and ADSCs were collected from patients who had undergone abdominal surgery...
March 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28343005/development-of-a-uv-crosslinked-biodegradable-hydrogel-containing-adipose-derived-stem-cells-to-promote-vascularization-for-skin-wounds-and-tissue-engineering
#20
Gozde Eke, Naside Mangir, Nesrin Hasirci, Sheila MacNeil, Vasif Hasirci
The aim of this study was to design a dermal substitute containing adipose derived stem cells (ADSC) that can be used to improve the regeneration of skin on difficult wound beds by stimulating rapid neovascularization. This was achieved by first synthesizing methacrylated gelatin (GelMA) and methacrylated hyaluronic acid (HAMA) precursors which could be stored at -80 (o)C after lyophilisation. Polymer precursors were then dissolved in media (in 15:1 ratio), ADSCs added together with the photoinitiator and crosslinked with 40 s of UV...
March 18, 2017: Biomaterials
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