keyword
MENU ▼
Read by QxMD icon Read
search

ADSC

keyword
https://www.readbyqxmd.com/read/28229424/three-dimensional-printed-scaffolds-with-multipotent-mesenchymal-stromal-cells-for-rabbit-mandibular-reconstruction-and-engineering
#1
Dongdong Fang, Michael Roskies, Mohamed-Nur Abdallah, Mohammed Bakkar, Jack Jordan, Li-Chieh Lin, Faleh Tamimi, Simon D Tran
Multipotent mesenchymal stromal cells (MSC) derived from both the bone marrow and adipose tissue possess the ability to differentiate into multiple cell lineages, regulate the immune function by secreting numerous bioactive paracrine factors, and hold great potential in cell therapy and tissue engineering. When combined with three-dimensional (3D) scaffolds, MSC can be used for bone defect reconstruction and engineering. This protocol describes the isolation of bone marrow mesenchymal stromal cells (BMMSC) and adipose-tissue derived stem cells (ADSC) from rabbits for subsequent seeding on tissue-engineered 3D-printed scaffolds and transplantation into a rabbit-model with the goal of repairing large osseous mandibular defects (one quarter of the lower jaw is removed surgically)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28229407/assessment-of-energy-metabolic-changes-in-adipose-tissue-derived-stem-cells
#2
Ghazaleh Hajmousa, Martin C Harmsen
Adipose tissue-derived stem cells (ADSC) are promising candidates for therapeutic applications in cardiovascular regenerative medicine. By definition, the phenotype ADSCs, e.g., the ubiquitous secretion of growth factors, cytokines, and extracellular matrix components is not met in vivo, which renders ADSC a culture "artefact." The medium constituents therefore impact the efficacy of ADSC. Little attention has been paid to the energy source in medium, i.e., glucose, which feeds the cell's power plants: mitochondria...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28220818/labeling-adipose-derived-stem-cell-sheet-by-ultrasmall-super-paramagnetic-fe3o4-nanoparticles-and-magnetic-resonance-tracking-in-vivo
#3
Shukui Zhou, Ting Yin, Qingsong Zou, Kaile Zhang, Guo Gao, Joseph G Shapter, Peng Huang, Qiang Fu
Cell sheet therapy has emerged as a potential therapeutic option for reparation and reconstruction of damaged tissues and organs. However, an effective means to assess the fate and distribution of transplanted cell sheets in a serial and noninvasive manner is still lacking. To investigate the feasibility of tracking Adipose derived stem cells (ADSCs) sheet in vivo using ultrasmall super-paramagnetic Fe3O4 nanoparticles (USPIO), canine ADSCs were cultured and incubated with USPIO and 0.75 μg/ml Poly-L-Lysine (PLL) for 12 h...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28219809/thermoresponsive-polymer-modified-microfibers-for-cell-separations
#4
Kenichi Nagase, Yoichi Sakurada, Satoru Onizuka, Takanori Iwata, Masayuki Yamato, Naoya Takeda, Teruo Okano
Thermoresponsive polymer-modified microfibers were prepared through electrospinning of poly(4-vinylbenzyl chloride) (PVBC) and subsequent surface-initiated atom transfer radical polymerization for grafting poly(N-isopropylacrylamide) (PIPAAm). Electrospinning conditions were optimized to produce large-diameter (20 μm) PVBC microfibers. The amount of PIPAAm grafted on the microfibers was controlled via the IPAAm monomer concentration. The microfibers exhibited thermally controlled cell separation by selective adhesion of normal human dermal fibroblasts in a mixed cell suspension that also contained human umbilical vein endothelial cells...
February 17, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28218774/osteoblast-differentiation-of-rabbit-adipose-derived-stem-cells-by-polyethylenimine-mediated-bmp-2-gene-transfection-in-vitro
#5
Q F Wang, Y Huang, G C He, H S Wang, Z H Chen, X H Cai, Y H Xie, Q Liu
The aim of this study was to examine the efficiency of polyethylenimine-mediated transfection of the human bone morphogenetic protein-2 (BMP-2) gene into rabbit adipose-derived stem cells (ADSCs), and its effect on osteoblast differentiation. Adipose tissue was isolated from the necks of adult Japanese white rabbits and cultured in vitro to obtain ADSCs. Gene delivery of BMP-2 was mediated by polyethylenimine and stable transformants were selected by G-418. The expression of BMP-2 mRNA was confirmed by reverse transcription-polymerase chain reaction, and of the BMP-2 protein by ELISA...
February 16, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28214341/angiopoietin-2-enhances-osteogenic-differentiation-of-bone-marrow-stem-cells
#6
Mi-Lan Kang, Eun-Ah Kim, Se-Young Jeong, Gun-Il Im
Our previous studies revealed that co-transplantation of bone marrow stem cells (BMSCs) and adipose-derived stem cells (ADSCs) can enhance bone regeneration and angiogenesis. However, it is unclear which genes are involved in the regulation of osteogenesis and/or angiogenesis during the co-culturing of BMSCs and ADSCs. The expression patterns of genes associated with osteogenesis and/or angiogenesis were analyzed in osteogenesis-induced BMSCs and ADSCs using an oligonucleotide microarray. Significant difference in the expression patterns of several genes were identified from hierarchical clustering and analyzed on co-cultured BMSCs and ADSCs...
February 18, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28211933/enhanced-bone-tissue-regeneration-using-a-3d-printed-microstructure-incorporated-with-a-hybrid-nano-hydrogel
#7
Dong Nyoung Heo, Nathan J Castro, Se-Jun Lee, Hanaul Noh, Wei Zhu, Lijie Grace Zhang
Three-dimensional (3D) functional constructs with biomimetic mechanical and chemical properties are ideal for various regenerative medicine applications. These properties of 3D fabricated constructs mainly depend on the intrinsic characteristics of the materials and fabrication method. In this respect, the current use of hydrogels for musculoskeletal tissue repair is not ideal due to the lack of suitable mechanical properties, as well as the high biomimetic requirement for success. To overcome this limitation, we developed a novel functionalized hydrogel with bioactive gold nanoparticles (GNPs), reinforcing a 3D printed microstructure via fused deposition modeling (FDM) for bone tissue regeneration...
February 17, 2017: Nanoscale
https://www.readbyqxmd.com/read/28205638/effects-of-mir-146a-on-the-osteogenesis-of-adipose-derived-mesenchymal-stem-cells-and-bone-regeneration
#8
Qing Xie, Wei Wei, Jing Ruan, Yi Ding, Ai Zhuang, Xiaoping Bi, Hao Sun, Ping Gu, Zi Wang, Xianqun Fan
Increasing evidence has indicated that bone morphogenetic protein 2 (BMP2) coordinates with microRNAs (miRNAs) to form intracellular networks regulating mesenchymal stem cells (MSCs) osteogenesis. This study aimed to identify specific miRNAs in rat adipose-derived mesenchymal stem cells (ADSCs) during BMP2-induced osteogenesis, we selected the most significantly down-regulated miRNA, miR-146a, to systematically investigate its role in regulating osteogenesis and bone regeneration. Overexpressing miR-146a notably repressed ADSC osteogenesis, whereas knocking down miR-146a greatly promoted this process...
February 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28202074/exendin-4-in-combination-with-adipose-derived-stem-cells-promotes-angiogenesis-and-improves-diabetic-wound-healing
#9
Eunhui Seo, Jae Soo Lim, Jin-Bum Jun, Woohyuk Choi, In-Sun Hong, Hee-Sook Jun
BACKGROUND: Diminished wound healing is a major complication of diabetes mellitus and can lead to foot ulcers. However, there are limited therapeutic methods to treat this condition. Exendin-4 (Ex-4), a glucagon-like peptide-1 receptor agonist, is known to have many beneficial effects on diabetes. In addition, mesenchymal stem cells are known to have wound healing effects. We investigated the effects of Ex-4 in combination with human adipose tissue-derived stem cells (ADSCs) on diabetic wound healing in a diabetic animal model...
February 15, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28201959/differentiation-potential-of-mesenchymal-stem-cells-derived-from-adipose-tissue-vs-bone-marrow-toward-annulus-fibrosus-cells-in-vitro
#10
Yifei Zhou, Xuqi Hu, Xuhao Zheng, Yaosen Wu, Naifeng Tian, Huazi Xu, Xiaolei Zhang
This study was to compare differentiation potential of bone marrow stem cells (BMSCs) and adipose-derived stem cells (ADSCs) from Sprague-Dawley rats towards annulus fibrosus (AF)-like cells. Using coculture plates with 0.4μm pore size polyethylene terephthalate (PET) track-etched inserts (Corning,USA), MSCs and AF cells were cocultured with direct cell-to-cell contact up till 21 days. We evaluated the AF markers expression at the histological level and molecular level. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) results showed up regulation in AF marker gene expressions of both two MSCs after coculture...
February 13, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28198165/vascular-endothelial-growth-factor-transfected-adipose-derived-stromal-cells-enhance-bone-regeneration-and-neovascularization-from-bone-marrow-stromal-cells
#11
Mi-Lan Kang, Ji-Eun Kim, Gun-Il Im
Vascular endothelial growth factor (VEGF)-transfected adipose-derived stromal cells (ADSCs(VEGF) ) were devised to promote bone regeneration and neovascularization of bone marrow stromal cells (BMSCs). ADSCs(VEGF) were added to BMSCs and cocultured in variable proportions. ADSCs(VEGF) alone or ADSCs(VEGF) with BMSCs (BMSCs:ADSCs(VEGF) ratio of 1:0.025-0.5) induced significantly greater tube formation in human umbilical vein endothelial cells than untransfected ADSCs. The cocultures of BMSCs and ADSCs(VEGF) at ratios of 1: 0...
February 15, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28192115/substance-p-enhances-proliferation-and-paracrine-potential-of-adipose-derived-stem-cells-in%C3%A2-vitro
#12
Suna Kim, Jiyuan Piao, Youngsook Son, Hyun Sook Hong
Stem cells have tremendous promise to treat intractable diseases. Notably, adipose-derived stem cells (ADSCs) are actively being investigated because of ease of sampling and high repopulation capacity in vitro. ADSCs can exert a therapeutic effect through differentiation and paracrine potential, and these actions have been proven in many diseases, including cutaneous and inflammatory diseases. Transplantation of ADSCs necessitates therapeutic quantities and thus, long term ex vivo culture of ADSCs. However, this procedure can impair the activity of ADSCs and provoke cellular senescence, leading to low efficacy in vivo...
February 10, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28187495/differential-hpv16-variant-distribution-in-squamous-cell-carcinoma-adenocarcinoma-and-adenosquamous-cell-carcinoma
#13
S Nicolás-Párraga, L Alemany, S de Sanjosé, F X Bosch, I G Bravo
Human Papillomavirus 16 (HPV16) causes 70% of invasive cervical cancers (ICC) worldwide. Interaction between HPV16 genetic diversity, host genetics and target tissue largely determine the chances to trigger carcinogenesis. We have analysed the differential prevalence of viral variants in 233 HPV16-monoinfected squamous (SCC), glandular (ADC) and mixed (ADSC) ICCs from four continents, assessing the contribution of geographical origin and cancer histology. We have further quantified the contribution of viral variants and cancer histology to differences in age at tumour diagnosis...
February 10, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28185267/regulation-of-adipose-tissue-derived-stromal-cell-orientation-and-motility-in-2d-and-3d-cultures-by-direct-current-electrical-field
#14
Gang Yang, Haiyan Long, Xiaomei Ren, Kunlong Ma, Zhenghua Xiao, Ying Wang, Yingqiang Guo
Cell alignment and motility play a critical role in a variety of cell behaviors, including cytoskeleton reorganization, membrane-protein relocation, nuclear gene expression, and extracellular matrix remodeling. Direct current electric field (EF) in vitro can direct many types of cells to align vertically to EF vector. In this work, we investigated the effects of EF stimulation on rat adipose-tissue-derived stromal cells (ADSCs) in 2D-culture on plastic culture dishes and in 3D-culture on various scaffold materials, including collagen hydrogels, chitosan hydrogels and poly(L-lactic acid)/gelatin electrospinning fibers...
February 10, 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28183632/a-potential-bioactive-wound-dressing-based-on-carboxymethyl-cellulose-zno-impregnated-mcm-41-nanocomposite-hydrogel
#15
Rasul Rakhshaei, Hassan Namazi
Lack of antibacterial activity, deficient water vapor and oxygen permeability, and insufficient mechanical properties are disadvantages of existing wound dressings. Hydrogels could absorb wound exudates due to their strong swelling ratio and give a cooling sensation and a wet environment. To overcome these shortcomings, flexible nanocomposite hydrogel films was prepared through combination of zinc oxide impregnated mesoporous silica (ZnO-MCM-41) as a nano drug carrier with carboxymethyl cellulose (CMC) hydrogel...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28173869/characteristics-of-human-adipose-derived-stem-cells-in-scleroderma-in-comparison-to-sex-and-age-matched-normal-controls-implications-for-regenerative-medicine
#16
Michelle Griffin, Caroline M Ryan, Omar Pathan, David Abraham, Christopher P Denton, Peter E M Butler
BACKGROUND: Adipose-derived stem cells (ADSCs) are emerging as an alternative stem cell source for cell-based therapies. Recent data suggest that autologous ADSC-enriched micrografting improves the effects of facial involvement in systemic sclerosis (SSc). We have extensively characterised ADSCs from SSc patients and compared their phenotype and function to healthy age- and sex-matched control ADSCs. METHODS: ADSCs were isolated and characterised from a cohort of six SSc patients (ADSC-SSc) and were compared to six healthy age- and sex-matched controls (ADSC-N)...
February 7, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28170200/glyoxalase-1-overexpression-reverses-defective-proangiogenic-function-of-diabetic-adipose-derived-stem-cells-in-streptozotocin-induced-diabetic-mice-model-of-critical-limb-ischemia
#17
Zhiyou Peng, Xinrui Yang, Jinbao Qin, Kaichuang Ye, Xin Wang, Huihua Shi, Mier Jiang, Xiaobing Liu, Xinwu Lu
Adipose-derived stem cell (ADSC)-based therapy is promising for critical limb ischemia (CLI) treatment, especially in patients with diabetes. However, the therapeutic effects of diabetic ADSCs (D-ADSCs) are impaired by the diabetes, possibly through intracellular reactive oxygen species (ROS) accumulation. The objective of the present study was to detect whether overexpression of methylglyoxal-metabolizing enzyme glyoxalase-1 (GLO1), which reduces ROS in D-ADSCs, can restore their proangiogenic function in a streptozotocin-induced diabetic mice model of CLI...
January 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28170198/differential-mechanisms-of-myocardial-conduction-slowing-by-adipose-tissue-derived-stromal-cells-derived-from-different-species
#18
Judith N Ten Sande, Nicoline W Smit, Mojtaba Parvizi, Shirley C M van Amersfoorth, Josée A Plantinga, Pascal F H M van Dessel, Jacques M T de Bakker, Marco C Harmsen, Ruben Coronel
Stem cell therapy is a promising therapeutic option to treat patients after myocardial infarction. However, the intramyocardial administration of large amounts of stem cells might generate a proarrhythmic substrate. Proarrhythmic effects can be explained by electrotonic and/or paracrine mechanisms. The narrow therapeutic time window for cell therapy and the presence of comorbidities limit the application of autologous cell therapy. The use of allogeneic or xenogeneic stem cells is a potential alternative to autologous cells, but differences in the proarrhythmic effects of adipose-derived stromal cells (ADSCs) across species are unknown...
January 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28170194/human-adipose-derived-stem-cells-expanded-under-ambient-oxygen-concentration-accumulate-oxidative-dna-lesions-and-experience-procarcinogenic-dna-replication-stress
#19
Rémy Bétous, Marie-Laure Renoud, Claire Hoede, Ignacio Gonzalez, Natalie Jones, Michel Longy, Luc Sensebé, Christophe Cazaux, Jean-Sébastien Hoffmann
Adipose-derived stem cells (ADSCs) have led to growing interest in cell-based therapy because they can be easily harvested from an abundant tissue. ADSCs must be expanded in vitro before transplantation. This essential step causes concerns about the safety of adult stem cells in terms of potential transformation. Tumorigenesis is driven in its earliest step by DNA replication stress, which is characterized by the accumulation of stalled DNA replication forks and activation of the DNA damage response. Thus, to evaluate the safety of ADSCs during ex vivo expansion, we monitored DNA replication under atmospheric (21%) or physiologic (1%) oxygen concentration...
January 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28167681/mir-450a-5p-within-rat-adipose-tissue-exosome-like-vesicles-promotes-adipogenic-differentiation-by-targeting-wisp2
#20
Yan Zhang, Mei Yu, Minjia Dai, Chang Chen, Qi Tang, Wei Jing, Hang Wang, Weidong Tian
Adipose tissue is an active endocrine organ that could secrete a wide number of factors to regulate adipogenesis via paracrine signals. In addition to soluble proteins in adipose tissue, microRNAs (miRNAs) enriched in extracellular vesicles (EVs), such as exosomes or microvesicles, could modulate intercellular communications. In this study, we demonstrated that exosome-like vesicles derived from adipose tissue (Exo-AT) were internalized by adipose tissue-derived stem cells (ADSCs), then induced adipogenesis...
February 6, 2017: Journal of Cell Science
keyword
keyword
99115
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"