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https://www.readbyqxmd.com/read/28109150/-isolation-culture-and-identification-of-adipose-derived-stem-cells-from-sd-rat-adipose-tissues-subjected-to-long-term-cryopreservation
#1
Qin Liu, Liping Wang, Fang Chen, Yi Zhang
Objective To study the feasibility of isolation and culture of adipose-derived stem cells (ADSCs) from SD rat adipose tissues subjected to long-term cryopreservation. Methods We took inguinal fat pads from healthy SD rats. Adipose tissues were stored with 100 mL/L dimethyl sulfoxide (DMSO) combined with 900 mL/L fetal bovine serum (FBS) in liquid nitrogen. Three months later, the adipose tissues were resuscitated for the isolation and culture of ADSCs. The growth status and morphology were observed. The growth curve and cell surface markers CD29, CD45, CD90 of the 3rd passage cells were analyzed respectively by CCK-8 assay and immunocytochemistry...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109058/in-vivo-construction-of-liver-tissue-by-implantation-of-a-hepatic-non-parenchymal-adipose-derived-stem-cell-sheet
#2
Mio Fujii, Kosho Yamanouchi, Yusuke Sakai, Zhassulan Baimakhanov, Izumi Yamaguchi, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Tamotsu Kuroki, Susumu Eguchi
Subcutaneous hepatocyte sheet implantation is an attractive therapeutic option for various liver diseases. However, this technique is limited by the availability of hepatocytes. Thus, the use of hepatic non-parenchymal cells (NPCs) containing small hepatocytes, which have the ability to proliferate more rapidly than mature hepatocytes, for transplantation has been suggested. The aim of our study was to construct liver tissue subcutaneously in rats by implanting NPC sheets co-cultivated with adipose-derived stem cells (ADSCs), which produce certain angiogenic factors...
January 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28107865/adipose-tissue-derived-stem-cells-ameliorates-dermal-fibrosis-in-a-mouse-model-of-scleroderma
#3
Wei Chen, Zhi-Kuan Xia, Man-Hui Zhang, Gui-Chun Ding, Xiao-Yan Zhang, Zheng-Xu Wang, Rong-Ya Yang
OBJECTIVE: To investigate the therapeutic potential of adipose-derived stem cells (ADSCs) for limited cutaneous scleroderma (LS) in mouse models. METHODS: ADSCs were isolated from pathogen-free female C57BL/6 mice and LS was induced in wild type (WT) C57BL/6 mice via daily injection of bleomycin (0.1 mL × 300 μg/mL) for 4 weeks; then the ADSCs were subcutaneously injected into the dorsal area in the model treatment group, and 100 μL of phosphate-buffered saline (PBS) solution was injected into the same site in the model control group...
January 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28107808/in-situ-release-of-vegf-enhances-osteogenesis-in-3d-porous-scaffolds-engineered-with-osterix-modified-adipose-derived-stem-cells
#4
Wan-Lin Xu, Hui-Shan Ong, Yun Zhu, Sheng-Wen Liu, Li-Min Liu, Kai-Hua Zhou, Zeng-Qi Xu, Jun Gao, Yan Zhang, Jin-Hai Ye, Wen-Jun Yang
Adipose-derived stem cells (ADSCs) can differentiate into various cell types and thus have great potential for regenerative medicine. Herein, rat ADSCs were isolated; transduced with lentiviruses expressing Osterix (Osx), a transcriptional factor essential for osteogenesis. Osx overexpression up-regulated key osteogenesis-related genes, such as special AT-rich binding protein 2, alkaline phosphatase, osteocalcin and osteopontin, at both the mRNA and protein levels. In addition, mineral nodule formation and alkaline phosphatase activity were enhanced in Osx-overexpressing ADSCs...
January 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28107665/a-%C3%AF-%C3%AF-conjugation-containing-soft-and-conductive-injectable-polymer-hydrogel-highly-efficiently-rebuilds-cardiac-function-after-myocardial-infarction
#5
Rui Bao, Baoyu Tan, Shuang Liang, Ning Zhang, Wei Wang, Wenguang Liu
Previous studies suggested that a stiffer hydrogel system exhibited a better performance to promote heart function after myocardial infarction (MI). However, the nature of myocardium, a tissue that alternately contracts and relaxes with electrical impulses, leads us to hypothesize that a soft and conductive hydrogel may be in favor of mechanical and electrical signals transmission to enhance heart function after MI. In this work, π-π conjugation interaction was first employed to produce a soft injectable hydrogel with conductive property...
January 12, 2017: Biomaterials
https://www.readbyqxmd.com/read/28092863/adipose-derived-stem-cells-ameliorate-renal-interstitial-fibrosis-through-inhibition-of-emt-and-inflammatory-response-via-tgf-%C3%AE-1-signaling-pathway
#6
Yan Song, Changliang Peng, Shasha Lv, Jing Cheng, Shanshan Liu, Qing Wen, Guangju Guan, Gang Liu
Adipose-derived stem cells (ADSCs) have been successfully used to treat acute kidney injury or acute renal failure. However, the effect of ADSCs on treating renal interstitial fibrosis remains unknown. Here, we assessed the therapeutic efficacy of ADSCs on renal interstitial fibrosis induced by unilateral ureter obstruction (UUO) and explored the potential mechanisms. After 7days of UUO, rats were injected with ADSCs (5×10(6)) or vehicle via tail vein. We found that ADSCs administration significantly ameliorated renal interstitial fibrosis, the occurrence of epithelial-mesenchymal transition (EMT) and inflammatory response...
January 13, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28086781/effects-of-the-intradiscal-implantation-of-stromal-vascular-fraction-plus-platelet-rich-plasma-in-patients-with-degenerative-disc-disease
#7
Comella Kristin, Silbert Robert, Parlo Michelle
BACKGROUND: Stromal vascular fraction (SVF) can easily be obtained from a mini-lipoaspirate procedure of fat tissue and platelet rich plasma (PRP) can be obtained from peripheral blood. The SVF contains a mixture of cells including ADSCs and growth factors and has been depleted of the adipocyte (fat cell) population. We evaluated the safety and efficacy of administering SVF and PRP intra-discally into patients with degenerative disc disease. METHODS: A total of 15 patients underwent a local tumescent liposuction procedure to remove approximately 60 ml of fat tissue...
January 13, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28056548/l-carnitine-contributes-to-enhancement-of-neurogenesis-from-mesenchymal-stem-cells-through-wnt-%C3%AE-catenin-and-pka-pathway
#8
Ezzatollah Fathi, Raheleh Farahzadi, Hojjatollah N Charoudeh
The identification of factors capable of enhancing neurogenesis has great potential for cellular therapies in neurodegenerative diseases. Multiple studies have shown the neuroprotective effects of L-carnitine (LC). This study determined whether neuronal differentiation of rat adipose tissue-derived mesenchymal stem cells (ADSCs) can be activated by LC. In this study, protein kinase A (PKA) and Wnt/β-catenin pathways were detected to show if this activation was due to these pathways. The expression of LC-induced neurogenesis markers in ADSCs was characterized using real-time PCR...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28054934/evaluation-of-the-potential-of-chitosan-%C3%AE-1-3-glucan-ha-material-as-scaffold-for-living-bone-graft-production-i-in-vitro-i-by-comparison-of-adsc-and-bmdsc-behaviour-on-its-surface
#9
Agata Przekora, Marta Vandrovcova, Martina Travnickova, Julia Pajorova, Martin Molitor, Grazyna Ginalska, Lucie Bacakova
The opinion regarding the origin of adult stem cells that should be used for living bone construct generation is strongly divided in the scientific community. In this study, the potential of chitosan/β-1,3-glucan/HA material as scaffold for bone regeneration applications was evaluated by behaviour comparison of adult stem cells derived from both origins - adipose tissue (ADSCs) and bone marrow (BMDSCs). In the case of ADSC isolation, low and high negative pressures were applied during liposuction procedure in order to determine if negative pressure settings may have impact on subsequent cell behaviour <i>in vitro</i>...
January 5, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28052725/paracrine-activity-from-adipose-derived-stem-cells-on-in-vitro-wound-healing-in-human-tympanic-membrane-keratinocytes
#10
Huan Ting Ong, Sharon Redmond, Robert Marano, Marcus Atlas, Magnus von Unge, Peder Aabel, Rodney J Dilley
Stem cell therapies for tympanic membrane repair have shown initial experimental success using mesenchymal stem cells in rat models to promote healing, however the mechanisms providing this benefit are not known. We investigated in vitro the paracrine effects of human adipose-derived stem cells (ADSC) on wound healing mechanisms for human tympanic membrane-derived keratinocytes (hTM) and immortalised human keratinocytes (HaCat). ADSC conditioned media (CMADSC) were assessed for paracrine activity on keratinocyte proliferation and migration, with hypoxic conditions for ADSC culture used to generate contrasting effects on cytokine gene expression...
January 4, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28039619/labeling-of-adipose-derived-stem-cells-with-quantum-dots-provides-stable-and-long-term-fluorescent-signal-for-ex-vivo-cell-tracking
#11
Clautina R M Costa, Matheus L T Feitosa, Dayseanny O Bezerra, Yulla K P Carvalho, Rodrigo F G Olivindo, Pablo B Fernando, Gustavo C Silva, Mirna L G Silva, Carlos E Ambrósio, Airton M Conde Júnior, Napoleão M Argolo Neto, Laís M Costa Silva, Maria A M Carvalho
Stem cells derived from adipose tissue (ADSC) have been used in cell therapy as an alternative to treat chronic and degenerative diseases. Using biomedical and image trials to track the cells when infused in the target tissue is essential to control cell migration and adhesion. The objective of the present study was to label and assess the adhesion of goat adipose tissue-derived stem cells (g-ADSC) after cell infusion in animal models by tracking luminescent intracytoplasmatic nanocrystals. The cells were labeled by using Qdots...
December 30, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28031116/-adipose-derived-stem-cell-transplantation-promotes-the-expression-of-netrin-1-in-the-rat-cortex-after-focal-cerebral-ischemia
#12
Jiehua Wang, Zhuquan Hong, Ying Pan, Guoqian Li
Objective To observe the effect of adipose-derived stem cells (ADSCs) transplantation on the expression of netrin-1 in rats after focal cerebral ischemia. Methods Male SD rats were randomly divided into control group, model group and ADSC group. ADSCs were harvested and purified. Focal cerebral ischemia models were established in rats by the suture method. ADSCs were injected into the lateral ventricle of ADSC group rats and the same does of PBS was given to model group rats. At day 4, 7 and 14 after reperfusion, six rats were sacrificed to remove the brain tissues at each time point...
January 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28017506/insulin-producing-cells-generation-by-overexpression-of-mir-375-in-adipose-derived-mesenchymal-stem-cells-from-diabetic-patients
#13
Mehran Piran, Seyed Ehsan Enderami, Mehrdad Piran, Hadis Soltani Sedeh, Ehsan Seyedjafari, Abdolreza Ardeshirylajimi
Diabetes Mellitus (DM) is a systematic disease, which happens because of destruction of islets of Langerhans in the pancreas and systematic resistance to insulin. The lack of donor for pancreas transplantation and risk of transplant rejection is the main challenge in the treatment of this disease. Stem cells are proper and sufficient source for creating insulin-producing cells (IPC). In this study adipose tissue was provided from diabetic patients operated for liposuction and then adipose derived stem cells (ADSCs) were isolated, characterized and then treated by lentiviruses containing miR-375, after 7, 14 and 21 days of induction, islet-like clusters (ILC) specific genes including insulin and PDX1 were evaluated by Real Time RT-PCR...
December 22, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28000114/evaluation-of-a-laminin-alginate-biomaterial-adipocytes-and-adipocyte-derived-stem-cells-interaction-in-animal-autologous-fat-grafting-model-using-7-tesla-magnetic-resonance-imaging
#14
Yo-Shen Chen, Yu-Sheng Hsueh, Yen-Yu Chen, Cheng-Yu Lo, Hao-Chih Tai, Feng-Huei Lin
Biomaterials are often added to autologous fat grafts both as supporting matrices for the grafted adipocytes and as cell carrier for adipose-derived stem cells (ADSCs). This in vivo study used an autologous fat graft model to test a lamininalginate biomaterial, adipocytes, and ADSCs in immune-competent rats. We transplanted different combinations of shredded autologous adipose tissue [designated "A" for adipose tissue]), laminin-alginate beads [designated "B" for bead], and ADSCs [designated "C" for cell]) into the backs of 15 Sprague-Dawley rats...
January 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/27992438/simultaneous-administration-of-adscs-based-therapy-and-gene-therapy-using-ad-hupa-reduces-experimental-liver-fibrosis
#15
Alejandra Meza-Ríos, Leonel García-Benavides, Jesus García-Bañuelos, Adriana Salazar-Montes, Juan Armendáriz-Borunda, Ana Sandoval-Rodríguez
BACKGROUND AND AIMS: hADSCs transplantation in cirrhosis models improves liver function and reduces fibrosis. In addition, Ad-huPA gene therapy diminished fibrosis and increased hepatocyte regeneration. In this study, we evaluate the combination of these therapies in an advanced liver fibrosis experimental model. METHODS: hADSCs were expanded and characterized before transplantation. Ad-huPA was simultaneously administrated via the ileac vein. Animals were immunosuppressed by CsA 24 h before treatment and until sacrifice at 10 days post-treatment...
2016: PloS One
https://www.readbyqxmd.com/read/27990180/the-effect-of-rgd-peptide-on-2d-and-miniaturized-3d-culture-of-hepm-cells-mscs-and-adscs-with-alginate-hydrogel
#16
Jenna Dumbleton, Pranay Agarwal, Haishui Huang, Nathaniel Hogrebe, Renzhi Han, Keith J Gooch, Xiaoming He
Advancements in tissue engineering require the development of new technologies to study cell behavior in vitro. This study focuses on stem cell behavior within various miniaturized three-dimensional (3D) culture conditions of alginate biomaterials modified with the Arg-Gly-Asp (RGD) peptide known for its role in cell adhesion/attachment. Human embryonic palatal mesenchyme (HEPM) cells, bone marrow derived mesenchymal stem cells (MSCs), and human adipose derived stem cells (ADSCs) were cultured on a flat hydrogel of different concentrations of alginate-RGD, and in the miniaturized 3D core of microcapsules with either a 2% alginate or 2% alginate-RGD shell...
June 2016: Cellular and Molecular Bioengineering
https://www.readbyqxmd.com/read/27987499/-therapeutic-effect-of-adipose-tissue-derived-stem-cells-on-bleomycin-induced-mice-of-scleroderma
#17
J L Dou, L Bai, C Y Pang, W L Zhang, W Zhang, Y F Wang
OBJECTIVE: To investigate the effects and mechanisms of adipose-derived stem cells (ADSCs) on bleomycin-induced mice of scleroderma. METHODS: In the study, 24 C57BL/6J female mice were randomly divided into control group, bleomycin(BLM)group, ADSCs (hypodermic injection) group and ADSCs (intravenous injection) group . BLM [2 mg/(kg×d)] was injected into the mice to establish the model of scleroderma. There were 6 mice in each group .The control group mice were injected with normal saline 2 mL/(kg×d) by subcutaneously...
December 18, 2016: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/27956359/chemical-group-dependent-plasma-polymerisation-preferentially-directs-adipose-stem-cell-differentiation-towards-osteogenic-or-chondrogenic-lineages
#18
M F Griffin, A Ibrahim, A M Seifalian, P E M Butler, D M Kalaskar, P Ferretti
: Human adipose derived stem cells (ADSCs) are being explored for the repair of craniofacial defects due to their multi-differentiation potential and ease of isolation and expansion. Crucial to using ADSCs for craniofacial repair is the availability of materials with appropriate biomechanical properties that can support their differentiation into bone and cartilage. We tested the hypothesis that different modifications of chemical groups on the surface of a nanocomposite polymer could increase human ADSC adhesion and selectively enhance their osteogenic and chondrogenic differentiation...
December 9, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27941495/evaluation-of-2-purification-methods-for-isolation-of-human-adipose-derived-stem-cells-based-on-red-blood-cell-lysis-with-ammonium-chloride-and-hypotonic-sodium-chloride-solution
#19
Sheng-Hong Li, Xuan Liao, Tian-En Zhou, Li-Ling Xiao, Yuan-Wen Chen, Fan Wu, Jing-Ru Wang, Biao Cheng, Jian-Xing Song, Hong-Wei Liu
BACKGROUND: The present study was conducted to compare 2 purification methods for isolation of human adipose-derived stromal vascular fraction or stem cells (ADSCs) based on red blood cell (RBC) lysis with 155 mM ammonium chloride (NH4Cl) and hypotonic sodium chloride (NaCl) solution, and try to develop a safe, convenient, and cost-effective purification method for clinical applications. METHODS: Adipose-derived stem cells and RBC were harvested from the fatty and fluid portions of liposuction aspirates, respectively...
January 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/27938473/are-the-adipose-derived-stem-cells-from-liver-falciform-ligaments-another-possible-source-of-mesenchymal-stem-cells
#20
Sang Lee, Jae Chong, Seon Min, Seon Bak, Kyung Kim
<p>Falciform ligaments in the liver are surrounded by adipose tissue. We investigated the capability of adipose derived stem cells from human liver falciform ligaments (hLF-ADSCs) to differentiate into hepatic type cells and confirm the functional capacity.</p><p>Mesenchymal stem cells (MSCs) were isolated from the liver falciform ligament and abdominal subcutaneous adipose tissue in patients undergoing partial hepatectomy for liver disease. Cells were cultivated in MSCs culture medium. Properties of MSCs were confirmed by flow cytometry, RT-PCR analysis, immunocytochemistry assays, and multi-lineage differentiation...
24, 2016: Cell Transplantation
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