keyword
MENU ▼
Read by QxMD icon Read
search

ADSC

keyword
https://www.readbyqxmd.com/read/29133512/exosomes-from-adipose-derived-stem-cells-attenuate-adipose-inflammation-and-obesity-through-polarizing-m2-macrophages-and-beiging-in-white-adipose-tissues
#1
Hui Zhao, Qianwen Shang, Zhenzhen Pan, Yang Bai, Zequn Li, Huiying Zhang, Qiu Zhang, Chun Guo, Lining Zhang, Qun Wang
Adipose-derived stem cells (ADSCs) play critical roles in controlling obesity-associated inflammation and metabolic disorders. Exosomes from ADSCs exert protective effects in several diseases, but their roles in obesity and related pathological conditions remain unclear. Here we showed that treatment of obese mice with ADSC-derived exosomes facilitated their metabolic homeostasis including improved insulin sensitivity (27.8% improvement), reduced obesity and alleviated hepatic steatosis. ADSC-derived exosomes drove M2 macrophage polarization, inflammation reduction and beiging in white adipose tissues (WAT) of diet-induced obese mice...
November 13, 2017: Diabetes
https://www.readbyqxmd.com/read/29131080/complete-static-repopulation-of-decellularized-porcine-tissues-for-heart-valve-engineering-an-in-vitro-study
#2
Annelies Roosens, Mahtab Asadian, Nathalie De Geyter, Pamela Somers, Ria Cornelissen
To date, a completely in vitro repopulated tissue-engineered heart valve has not been developed. This study focused on sequentially seeding 2 cell populations onto porcine decellularized heart valve leaflets (HVL) and pericardia (PER) to obtain fully repopulated tissues. For repopulation of the interstitium, porcine valvular interstitial cells (VIC) and bone marrow-derived mesenchymal stem cells (BM-MSC) or adipose tissue-derived stem cells (ADSC) were used. In parallel, the culture medium was supplemented with ascorbic acid 2-phosphate (AA) and its effect on recolonization was investigated...
November 8, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29129620/human-adipose-derived-stem-cells-adsc-and-human-periodontal-ligament-stem-cells-pdlsc-as-cellular-substrates-of-a-toxicity-prediction-assay
#3
Natássia Caroline Resende Corrêa, Crisciele Kuligovski, Ariane Caroline Campos Paschoal, Ana Paula Ressetti Abud, Carmen Lucia Kuniyoshi Rebelatto, Lidiane Maria Boldrini Leite, Alexandra Cristina Senegaglia, Bruno Dallagiovanna, Alessandra Melo de Aguiar
With the increasing need to develop in vitro assays to replace animal use, human stem cell-derived methods are emerging and showing outstanding contributions to the toxicological screening of substances. Adult human stem cells such as adipose-derived stem cells (ADSC) and periodontal ligament stem cells (PDLSC) were used as cell substrates for a cytotoxicity assay and toxicity prediction using the neutral red uptake (NRU) assay. First, primary cell cultures from three independent donors, from each tissue source, were characterized as mesenchymal stem cells (MSC) by plastic adherence and appropriate immunophenotype for MSC markers (positive for CD90, CD73, and CD105 and negative for CD11b, CD34, CD45, HLADR, and CD19)...
November 9, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/29128538/hyaluronan-size-alters-chondrogenesis-of-adipose-derived-stem-cells-via-the-cd44-erk-sox-9-pathway
#4
Shun-Cheng Wu, Chung-Hwan Chen, Jyun-Ya Wang, Yi-Shan Lin, Je-Ken Chang, Mei-Ling Ho
Hyaluronan (HA) is a natural linear polymer that is one of the main types of extracellular matrix during the early stage of chondrogenesis. We found that the chondrogenesis of adipose-derived stem cells (ADSCs) can be initiated and promoted by the application of HA to mimic the chondrogenic niche. The aim of this study is to investigate the optimal HA molecular weight (Mw) for chondrogenesis of ADSCs and the detailed mechanism. In this study, we investigated the relationships among HA Mw, CD44 clustering, and the extracellular signal-regulated kinase (ERK)/SOX-9 pathway during chondrogenesis of ADSCs...
November 8, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29127873/local-angiotensin-ii-promotes-adipogenic-differentiation-of-human-adipose-tissue-mesenchymal-stem-cells-through-type-2-angiotensin-receptor
#5
Veronika Y Sysoeva, Liudmila V Ageeva, Pyotr A Tyurin-Kuzmin, George V Sharonov, Daniyar T Dyikanov, Natalia I Kalinina, Vsevolod A Tkachuk
Obesity is often associated with high systemic and local activity of renin-angiotensin system (RAS). Mesenchymal stem cells of adipose tissue are the main source of adipocytes. The aim of this study was to clarify how local RAS could control adipose differentiation of human adipose tissue derived mesenchymal stem cells (ADSCs). We examined the distribution of angiotensin receptor expressing cells in human adipose tissue and found that type 1 and type 2 receptors are co-expressed in its stromal compartment, which is known to contain mesenchymal stem cells...
November 3, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29115445/mir%C3%A2-126-is-essential-for-endothelial-phenotype-expression-during-endothelial-differentiation-in-adipose%C3%A2-derived-stem-cells
#6
Han Xie, Fang Wang, Xiao Chen, Hao Ying
The endothelial differentiation of stem cells serves an essential role in vascular development, function and disease. Autograft adipose‑derived stem cells (ADSCs) may be a novel source of cell for use in the study of angiogenesis. microRNA‑126 (miR‑126) has been extensively studied in endothelial cells. However, the effect of miR‑126 on the endothelial differentiation of ADSCs has not been demonstrated. In the present study, it was observed that the expression of miR‑126 was markedly increased during the endothelial differentiation of ADSCs in a time‑dependent manner...
October 27, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29115380/combination-treatment-of-adipose-derived-stem-cells-and-adiponectin-attenuates-pulmonary-arterial-hypertension-in-rats-by-inhibiting-pulmonary-arterial-smooth-muscle-cell-proliferation-and-regulating-the-ampk-bmp-smad-pathway
#7
Li Luo, Wuhong Zheng, Guili Lian, Huaning Chen, Ling Li, Changsheng Xu, Liangdi Xie
The present study aimed to assess the effects of therapy with adiponectin (APN) gene-modified adipose-derived stem cells (ADSCs) on pulmonary arterial hypertension (PAH) in rats and the underlying cellular and molecular mechanisms. ADSCs were successfully isolated from the rats and characterized. ADSCs were effectively infected with the green fluorescent protein (GFP)-empty (ADSCs-V) or the APN-GFP (ADSCs-APN) lentivirus and the APN expression was evaluated by ELISA. Sprague-Dawley rats were administered monocrotaline (MCT) to develop PAH...
October 31, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29115374/metabolic-profiles-of-adipose-derived-and-bone-marrow-derived-stromal-cells-from-elderly-coronary-heart-disease-patients-by-capillary-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#8
Jian-Zhong Li, Hui Qu, Jian Wu, Fan Zhang, Zhi-Bo Jia, Jian-Yong Sun, Bo Lv, Yue Kang, Shu-Lin Jiang, Kai Kang
Adipose-tissue derived mesenchymal stem cell (ADSC)-based therapy is a promising option for patients with atherosclerotic conditions, including coronary artery disease. However, the potential differences in the metabolic characteristics between bone marrow-derived mesenchymal stem cells (BMSCs) and ADSCs have remained to be fully elucidated. The present study aimed to compare the metabolic profiles of BMSCs and ADSCs via liquid chromatography quadrupole time-of-flight mass spectrometry. BMSCs and ADSCs obtained from elderly coronary heart disease patients were cultured, and after three passages, supernatants of each cell type were collected and systematically analysed...
October 19, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29110545/serum-from-ccl4-induced-acute-rat-injury-model-induces-differentiation-of-adscs-towards-hepatic-cells-and-reduces-liver-fibrosis
#9
Maria Tayyab Baig, Gibran Ali, Sana Javaid Awan, Umara Shehzad, Azra Mehmood, Sadia Mohsin, Shaheen N Khan, Sheikh Riazuddin
Cellular therapies hold promise to alleviate liver diseases. This study explored the potential of allogenic serum isolated from rat with acute CCl4 injury to differentiate adipose derived stem cells (ADSCs) towards hepatic lineage. Acute liver injury was induced by CCl4 which caused significant increase in serum levels of VEGF, SDF1α and EGF. ADSCs were preconditioned with 3% serum isolated from normal and acute liver injury models. ADSCs showed enhanced expression of hepatic markers (AFP, albumin, CK8 and CK19)...
November 7, 2017: Growth Factors
https://www.readbyqxmd.com/read/29105402/adipose-derived-stem-cells-affect-mir-145-and-p53-expressions-of-co-cultured-hematopoietic-stem-cells
#10
Tahereh Foroutan, Aisan Farhadi, Saeed Abroun, Bahram Mohammad Soltani
OBJECTIVES: Umbilical cord blood is used for transplantation purposes in regenerative medicine of hematological disorders. MicroRNAs are important regulators of gene expression that control both physiological and pathological processes such as cancer development and incidence. There is a new relation between p53 (tumor suppressor gene) and miR-145 (suppressor of cell growth) upregulation. In this study, we have assessed how adipose-derived stem cells (ADSCs) affect the expansion of hematopoietic stem cells (HSCs), as well as miR-145 and p53 expressions...
January 2018: Cell Journal
https://www.readbyqxmd.com/read/29104736/aldehyde-dehydrogenase-activity-helps-identify-a-subpopulation-of-murine-adipose-derived-stem-cells-with-enhanced-adipogenic-and-osteogenic-differentiation-potential
#11
Harumichi Itoh, Shimpei Nishikawa, Tomoya Haraguchi, Yu Arikawa, Shotaro Eto, Masato Hiyama, Toshie Iseri, Yoshiki Itoh, Munekazu Nakaichi, Yusuke Sakai, Kenji Tani, Yasuho Taura, Kazuhito Itamoto
AIM: To identify and characterize functionally distinct subpopulation of adipose-derived stem cells (ADSCs). METHODS: ADSCs cultured from mouse subcutaneous adipose tissue were sorted fluorescence-activated cell sorter based on aldehyde dehydrogenase (ALDH) activity, a widely used stem cell marker. Differentiation potentials were analyzed by utilizing immunocytofluorescece and its quantitative analysis. RESULTS: Approximately 15% of bulk ADSCs showed high ALDH activity in flow cytometric analysis...
October 26, 2017: World Journal of Stem Cells
https://www.readbyqxmd.com/read/29104428/adipose-derived-stem-cells-in-novel-approaches-to-breast-reconstruction-their-suitability-for-tissue-engineering-and-oncological-safety
#12
REVIEW
Niamh O'Halloran, Donald Courtney, Michael J Kerin, Aoife J Lowery
Adipose-derived stem cells (ADSCs) are rapidly becoming the gold standard cell source for tissue engineering strategies and hold great potential for novel breast reconstruction strategies. However, their use in patients with breast cancer is controversial and their oncological safety, particularly in relation to local disease recurrence, has been questioned. In vitro, in vivo, and clinical studies using ADSCs report conflicting data on their suitability for adipose tissue regeneration in patients with cancer...
2017: Breast Cancer: Basic and Clinical Research
https://www.readbyqxmd.com/read/29097870/adipose-derived-stromal-cells-resemble-bone-marrow-stromal-cells-in-hepatocyte-differentiation-potential-in-vitro-and-in-vivo
#13
Li-Juan Xu, Shu-Fang Wang, De-Qing Wang, Lian-Jun Ma, Zheng Chen, Qian-Qian Chen, Jun Wang, Li Yan
AIM: To investigate whether mesenchymal stem cells (MSCs) from adipose-derived stromal cells (ADSCs) and bone marrow stromal cells (BMSCs) have similar hepatic differentiation potential. METHODS: Mouse ADSCs and BMSCs were isolated and cultured. Their morphological and phenotypic characteristics, as well as their multiple differentiation capacity were compared. A new culture system was established to induce ADSCs and BMSCs into functional hepatocytes. Reverse transcription polymerase chain reaction, Western blot, and immunofluorescence analyses were performed to identify the induced hepatocyte-like cells...
October 14, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29097816/anti-inflammatory-and-anti-fibrotic-effects-of-intravenous-adipose-derived-stem-cell-transplantation-in-a-mouse-model-of-bleomycin-induced-interstitial-pneumonia
#14
Takuya Kotani, Ryota Masutani, Takayasu Suzuka, Katsuhiro Oda, Shigeki Makino, Masaaki Ii
Adipose-derived stem cells (AdSCs) have recently been considered a useful treatment tool for autoimmune disease because of their anti-inflammatory and immunosuppressive effects. We investigated the therapeutic effect of intravenous AdSC transplantation in a mouse model of bleomycin-induced lung injury. AdSCs accumulated in the pulmonary interstitium and inhibited both inflammation and fibrosis in the lung, markedly improving the survival rate of mice with bleomycin-induced lung injury in a cell number-dependent manner...
November 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29096855/impact-of-omega-3-fatty-acids-on-expression-of-angiogenic-cytokines-and-angiogenesis-by-adipose-derived-stem-cells
#15
Iryna Parshyna, Susann Lehmann, Katrin Grahl, Claudia Pahlke, Annika Frenzel, Helmut Weidlich, Henning Morawietz
BACKGROUND AND AIMS: Human adipose-tissue derived stem cells (ADSC) are interesting novel targets in tissue engineering and regenerative medicine with pronounced angiogenic capacities. Furthermore, omega-3 fatty acids have been described to mediate cardioprotective effects, but their role in angiogenesis and vascular regeneration is not well-understood. Here, we analyzed the impact of different omega-3 fatty acids on angiogenesis by ADSCs. METHODS: Stem cells were cultured as monolayers or in 3D models, in spheroids embedded in collagen matrix or in co-cultures with human umbilical vein endothelial cells (HUVECs) in the Matrigel™ assay...
November 2017: Atherosclerosis. Supplements
https://www.readbyqxmd.com/read/29090002/adipose-derived-mesenchymal-stem-cells-accelerate-nerve-regeneration-and-functional-recovery-in-a-rat-model-of-recurrent-laryngeal-nerve-injury
#16
Yun Li, Wen Xu, Li-Yu Cheng
Medialization thyroplasty or injection laryngoplasty for unilateral vocal fold paralysis cannot restore mobility of the vocal fold. Recent studies have shown that transplantation of mesenchymal stem cells is effective in the repair of nerve injuries. This study investigated whether adipose-derived stem cell transplantation could repair recurrent laryngeal nerve injury. Rat models of recurrent laryngeal nerve injury were established by crushing with micro forceps. Adipose-derived mesenchymal stem cells (ADSCs; 8 × 10(5)) or differentiated Schwann-like adipose-derived mesenchymal stem cells (dADSCs; 8 × 10(5)) or extracellular matrix were injected at the site of injury...
September 2017: Neural Regeneration Research
https://www.readbyqxmd.com/read/29082503/an-endothelial-cell-mesenchymal-stem-cell-coculture-cord-formation-assay-to-model-vascular-biology-in-vitro
#17
Michelle Swearingen, Beverly Falcon, Sudhakar Chintharlapalli, Mark Uhlik
Blood vessels are crucial components for normal tissue development and homeostasis, so it is not surprising that endothelial dysfunction and dysregulation results in a variety of different pathophysiological conditions. The large number of vascular-related disorders and the emergence of angiogenesis as a major hallmark of cancer has led to significant interest in the development of drugs that target the vasculature. While several in vivo models exist to study developmental and pathological states of blood vessels, few in vitro assays have been developed that capture the significant complexity of the vascular microenvironment...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29082349/ell3-modulates-the-wound-healing-activity-of-conditioned-medium-of-adipose-derived-stem-cells
#18
Jae-Yong Lee, Nuri Oh, Kyung-Soon Park
While adipose-derived stem cell-conditioned medium (ADSC-CM) has been demonstrated to promote skin wound healing, the mechanism regulating this effect remains unelucidated. In this study, we aimed to investigate the role of Ell3 in the wound healing activity of ADSC-CM. In vitro analysis revealed that Ell3 suppression in ADSCs impairs the promotive activity of ADSC-CM on the proliferation and migration of mouse embryonic fibroblasts (MEF) and normal human dermal fibroblasts (NHDF). Consistently, the expression of MMP family genes, which regulate cell proliferation and migration, was significantly suppressed in MEF and NHDF treated with siEll3-transfected ADSC-CM...
September 2017: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/29075508/effect-of-alginate-concentration-on-chondrogenesis-of-co-cultured-human-adipose-derived-stem-cells-and-nasal-chondrocytes-a-biological-study
#19
Y W Ewa-Choy, B Pingguan-Murphy, N A Abdul-Ghani, J Jahendran, K H Chua
BACKGROUND: The three-dimensional (3D) system is one of the important factors to engineer a biocompatible and functional scaffold for the applications of cell-based therapies for cartilage repair. The 3D alginate hydrogels system has previously been shown to potentially promote chondrogenesis. The chondrocytic differentiation of co-cultured adipose-derived stem cells (ADSCs) and nasal chondrocytes (NCs) within alginate constructs are hypothesized to be influenced by concentration of alginate hydrogel...
2017: Biomaterials Research
https://www.readbyqxmd.com/read/29067774/recellularization-of-well-preserved-decellularized-kidney-scaffold-using-adipose-tissue-derived-stem-cells
#20
Aibing Xue, Guangzhu Niu, Yuan Chen, Kailin Li, Zhiying Xiao, Yun Luan, Chao Sun, Xiaoshuai Xie, Denglu Zhang, Xiaohang Du, Feng Kong, Yanxia Guo, Haiyang Zhang, Guanghui Cheng, Qian Xin, Yong Guan, Shengtian Zhao
To establish a recellularization kidney model by using adipose tissue-derived stem cells (ADSCs) as seeding cells and to investigate the growth and differentiation of ADSCs in decellularized kidney scaffolds. ADSCs were isolated using a modified method and then identified using flow cytometry analysis. Osteogenesis and adipogenesis differentiation were performed. Rat kidneys were decellularized using 0.5% sodium dodecyl sulfate (SDS). Immunofluorescence, immunohistochemisry and scanning electron microscope were conducted to examine the scaffold microstructure...
October 25, 2017: Journal of Biomedical Materials Research. Part A
keyword
keyword
99115
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"