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Outer membrane vesicles

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https://www.readbyqxmd.com/read/29240272/amino-acid-deprivation-and-central-carbon-metabolism-regulate-the-production-of-outer-membrane-vesicles-and-tubes-by-francisella
#1
Vinaya Sampath, William D McCaig, David G Thanassi
Francisella tularensis is a highly virulent Gram-negative bacterial pathogen that causes the zoonotic disease tularemia. F. novicida, a model tularemia strain, produces spherical outer membrane vesicles (OMV), as well as novel tubular vesicles and extensions of the cell surface. These OMV and tubes (OMV/T) are produced in a regulated manner and contain known virulence factors. Mechanisms by which bacterial vesicles are produced and regulated are not well understood. We performed a genetic screen in F. novicida to decipher the molecular basis for regulated OMV/T formation, and identified both hypo- and hyper-vesiculating mutants...
December 14, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/29234030/escherichia-coli-outer-membrane-vesicles-can-contribute-to-sepsis-induced-cardiac-dysfunction
#2
Kristina Svennerholm, Kyong-Su Park, Johannes Wikström, Cecilia Lässer, Rossella Crescitelli, Ganesh V Shelke, Su Chul Jang, Shintaro Suzuki, Elga Bandeira, Charlotta S Olofsson, Jan Lötvall
Sepsis induced cardiac dysfunction (SIC) is a severe complication to sepsis which significantly worsens patient outcomes. It is known that bacteria have the capacity to release outer membrane vesicles (OMVs), which are nano-sized bilayered vesicles composed of lipids and proteins, that can induce a fatal inflammatory response. The aim of this study was to determine whether OMVs from a uropathogenic Escherichia coli strain can induce cardiac dysfunction, and to elucidate any mechanisms involved. OMVs induced irregular Ca2+ oscillations with a decreased frequency in cardiomyocytes through recordings of intracellular Ca2+ dynamics...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29232837/a-burkholderia-pseudomallei-outer-membrane-vesicle-vaccine-provides-cross-protection-against-inhalational-glanders-in-mice-and-non-human-primates
#3
Sarah M Baker, Christopher J H Davitt, Natalya Motyka, Nicole L Kikendall, Kasi Russell-Lodrigue, Chad J Roy, Lisa A Morici
Burkholderia mallei is a Gram-negative, non-motile, facultative intracellular bacillus and the causative agent of glanders, a highly contagious zoonotic disease. B. mallei is naturally resistant to multiple antibiotics and there is concern for its potential use as a bioweapon, making the development of a vaccine against B. mallei of critical importance. We have previously demonstrated that immunization with multivalent outer membrane vesicles (OMV) derived from B. pseudomallei provide significant protection against pneumonic melioidosis...
December 9, 2017: Vaccines
https://www.readbyqxmd.com/read/29232134/localisation-of-cholesterol-within-supported-lipid-bilayers-made-of-a-natural-extract-of-tailor-deuterated-phosphatidylcholine
#4
Sarah Waldie, Tania Kjellerup Lind, Kathryn Louise Browning, Martine Moulin, Michael Haertlein, Victor Trevor Forsyth, Alessandra Luchini, Gernot A Strohmeier, Harald Pichler, Selma Maric, Marite Cardenas
Cholesterol is an essential component of mammalian membranes that is known to induce a series of physicochemical changes in the lipid bilayer. Such changes include the formation of liquid ordered phases with an increased thickness and configurational order as compared to liquid disordered phases. For saturated lipid membranes, cholesterol molecules localise close to the lipid head group-tail interface. However, the presence of polyunsaturated lipids was recently shown to promote the relocation of cholesterol towards the inner interface between the two bilayer leaflets...
December 12, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29230043/nanoparticle-orientation-to-control-rna%C3%A2-loading-and-ligand-display-on-extracellular-vesicles-for-cancer-regression
#5
Fengmei Pi, Daniel W Binzel, Tae Jin Lee, Zhefeng Li, Meiyan Sun, Piotr Rychahou, Hui Li, Farzin Haque, Shaoying Wang, Carlo M Croce, Bin Guo, B Mark Evers, Peixuan Guo
Nanotechnology offers many benefits, and here we report an advantage of applying RNA nanotechnology for directional control. The orientation of arrow-shaped RNA was altered to control ligand display on extracellular vesicle membranes for specific cell targeting, or to regulate intracellular trafficking of small interfering RNA (siRNA) or microRNA (miRNA). Placing membrane-anchoring cholesterol at the tail of the arrow results in display of RNA aptamer or folate on the outer surface of the extracellular vesicle...
December 11, 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/29229778/structural-and-functional-insights-into-the-role-of-bamd-and-bame-within-the-%C3%AE-barrel-assembly-machinery-in-neisseria-gonorrhoeae
#6
Aleksandra E Sikora, Igor H Wierzbicki, Ryszard A Zielke, Rachael F Ryner, Konstantin V Korotkov, Susan K Buchanan, Nicholas Noinaj
The β-barrel assembly machinery (BAM) is a conserved multi-component protein complex responsible for the biogenesis of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria. Given its role in the production of OMPs for survival and pathogenesis, BAM represents an attractive target for the development of therapeutic interventions including drugs and vaccines against multi-drug resistant bacteria such as Neisseria gonorrhoeae The first structure of BamA, the central component of BAM, was from N...
December 11, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29208444/characterization-of-outer-membrane-vesicles-of-acetobacter-pasteurianus-nbrc3283
#7
Masahito Hashimoto, Taichi Matsumoto, Miwa Tamura-Nakano, Mami Ozono, Shuhei Hashiguchi, Yasuo Suda
Acetobacter pasteurianus is characterized as a fermenting bacterium of kurozu, which is a common traditional Japanese black vinegar. Recently, we separated acid-resistant and low Toll-like receptor 4 (TLR4)-stimulatory lipopolysaccharides (LPS) from A. pasteurianus. We also showed that their lipid A parts possessed a novel sugar backbone that is responsible for the low TLR4-stimulatory and acid-resistant properties of the LPS. Outer membrane vesicles (OMVs) are nano-sized spherical structures secreted from many gram-negative bacteria...
December 2, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29205766/the-bile-salt-sodium-taurocholate-induces-campylobacter-jejuni-outer-membrane-vesicle-production-and-increases-omv-associated-proteolytic-activity
#8
Abdi Elmi, Amber Dorey, Eleanor Watson, Heena Jagatia, Neil F Inglis, Ozan Gundogdu, Mona Bajaj-Elliott, Brendan W Wren, David G E Smith, Nick Dorrell
Campylobacter jejuni, the leading cause of bacterial acute gastroenteritis worldwide, secretes an arsenal of virulence-associated proteins within outer membrane vesicles (OMVs). C. jejuni OMVs contain three serine proteases (HtrA, Cj0511 and Cj1365c) which cleave the intestinal epithelial cell (IEC) tight and adherens junction proteins occludin and E-cadherin, promoting enhanced C. jejuni adhesion to and invasion of IECs. C. jejuni OMVs also induce IECs innate immune responses. The bile salt sodium taurocholate (ST) is sensed as a host signal to co-ordinate the activation of virulence-associated genes in the enteric pathogen Vibrio cholerae...
December 4, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/29205725/pleckstrin-homology-domain-of-p210-bcr-abl-interacts-with-cardiolipin-to-regulate-its-mitochondrial-translocation-and-subsequent-mitophagy
#9
Kentaro Shimasaki, Miho Watanabe-Takahashi, Masato Umeda, Satoru Funamoto, Yoshiro Saito, Noriko Noguchi, Keigo Kumagai, Kentaro Hanada, Fujiko Tsukahara, Yoshiro Maru, Norihito Shibata, Mikihiko Naito, Kiyotaka Nishikawa
Chronic myeloid leukemia (CML) is caused by the chimeric protein p210 BCR-ABL encoded by a gene on the Philadelphia chromosome. Although the kinase domain of p210 BCR-ABL is an active driver of CML, the pathological role of its pleckstrin homology (PH) domain remains unclear. Here, we carried out phospholipid vesicle-binding assays to show that cardiolipin (CL), a characteristic mitochondrial phospholipid, is a unique ligand of the PH domain. Arg726, a basic amino acid in the ligand-binding region, was crucial for ligand recognition...
December 5, 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/29203497/outer-membrane-vesicles-omv-based-and-proteomics-driven-antigen-selection-identifies-novel-factors-contributing-to-bordetella-pertussis-adhesion-to-epithelial-cells
#10
Gianmarco Gasperini, Massimiliano Biagini, Vanessa Arato, Claudia Gianfaldoni, Alessandro Vadi, Nathalie Norais, Giuliano Bensi, Isabel Delany, Mariagrazia Pizza, Beatrice Aricò, Rosanna Leuzzi
Despite high vaccination coverage world-wide, whooping cough, a highly contagious disease caused by Bordetella pertussis, is recently increasing in occurrence suggesting that novel vaccine formulations targeted at the prevention of colonization and transmission should be investigated. In order to identify new candidates for inclusion in the acellular formulation, we used spontaneously released outer membrane vesicles (OMV) as a potential source of key adhesins. The enrichment of Bvg+ OMV with adhesins and the ability of anti-OMV serum to inhibit the adhesion of B...
December 4, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29191736/immunogenicity-of-vibrio-cholerae-outer-membrane-vesicles-secreted-at-various-environmental-conditions
#11
Rezaei Adriani, Seyed Latif Mousavi Gargari, Shahram Nazarian, Samaneh Sarvary, Nafiseh Noroozi
Cholera is caused by toxigenic Vibrio cholerae. It is a significant health problem and an important cause of mortality of children in developing countries. Annually, about 5-7 million people are being infected worldwide, leading to death of 100,000 to 120,000. Immunization using the currently available cholera vaccines has been recommended by World Health Organization (WHO) in areas where cholera is endemic or at risk of outbreaks. Gram-negative bacteria secrete outer membrane vesicles (OMVs) that play important roles in virulence and host-pathogen interaction...
November 27, 2017: Vaccine
https://www.readbyqxmd.com/read/29186822/venture-from-the-interior-herpesvirus-pul31-escorts-capsids-from-nucleoplasmic-replication-compartments-to-sites-of-primary-envelopment-at-the-inner-nuclear-membrane
#12
REVIEW
(no author information available yet)
Herpesviral capsid assembly is initiated in the nucleoplasm of the infected cell. Size constraints require that newly formed viral nucleocapsids leave the nucleus by an evolutionarily conserved vescular transport mechanism called nuclear egress. Mature capsids released from the nucleoplasm are engaged in a membrane-mediated budding process, composed of primary envelopment at the inner nuclear membrane and de-envelopment at the outer nuclear membrane. Once in the cytoplasm, the capsids receive their secondary envelope for maturation into infectious virions...
November 25, 2017: Cells
https://www.readbyqxmd.com/read/29186191/lipopolysaccharide-structure-impacts-the-entry-kinetics-of-bacterial-outer-membrane-vesicles-into-host-cells
#13
Eloise J O'Donoghue, Natalie Sirisaengtaksin, Douglas F Browning, Ewa Bielska, Mohammed Hadis, Francisco Fernandez-Trillo, Luke Alderwick, Sara Jabbari, Anne Marie Krachler
Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis. However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells. Here, we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe...
November 29, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29180369/membrane-vesicles-derived-from-bordetella-bronchiseptica-active-constituent-of-a-new-vaccine-against-infections-caused-by-this-pathogen
#14
D Bottero, M E Zurita, M E Gaillard, E Bartel, C Vercellini, D Hozbor
Bordetella bronchiseptica, a Gram-negative bacterium, causes chronic respiratory-tract infections in a wide variety of mammalian hosts-humans included, albeit rarely. We recently designed B. pertussis and B. parapertussis experimental vaccines based on outer-membrane vesicles derived from each pathogen and obtained protection against the respective infections in mice. Here, we demonstrate that outer-membrane vesicles derived from virulent-phase B. bronchiseptica (OMVBbvir+) protect mice against sublethal infection from different B...
November 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29180031/will-we-have-new-pertussis-vaccines
#15
Camille Locht
Despite wide vaccination coverage with efficacious vaccines, pertussis is still not under control in any country. Two types of vaccines are available for the primary vaccination series, diphtheria/tetanus/whole-cell pertussis and diphtheria/tetanus/acellular pertussis vaccines, in addition to reduced antigen content vaccines recommended for booster vaccination. Using these vaccines, several strategies are being explored to counter the current pertussis problems, including repeated vaccination, cocoon vaccination and maternal immunization...
November 24, 2017: Vaccine
https://www.readbyqxmd.com/read/29164053/synergistic-protective-activity-of-tumor-specific-epitopes-engineered-in-bacterial-outer-membrane-vesicles
#16
Alberto Grandi, Michele Tomasi, Ilaria Zanella, Luisa Ganfini, Elena Caproni, Laura Fantappiè, Carmela Irene, Luca Frattini, Samine J Isaac, Enrico König, Francesca Zerbini, Simona Tavarini, Chiara Sammicheli, Fabiola Giusti, Ilaria Ferlenghi, Matteo Parri, Guido Grandi
Introduction: Bacterial outer membrane vesicles (OMVs) are naturally produced by all Gram-negative bacteria and, thanks to their plasticity and unique adjuvanticity, are emerging as an attractive vaccine platform. To test the applicability of OMVs in cancer immunotherapy, we decorated them with either one or two protective epitopes present in the B16F10EGFRvIII cell line and tested the protective activity of OMV immunization in C57BL/6 mice challenged with B16F10EGFRvIII. Materials and methods: The 14 amino acid B cell epitope of human epidermal growth factor receptor variant III (EGFRvIII) and the mutation-derived CD4+ T cell neo-epitope of kif18b gene (B16-M30) were used to decorate OMVs either alone or in combination...
2017: Frontiers in Oncology
https://www.readbyqxmd.com/read/29163452/the-opportunistic-pathogen-vibrio-vulnificus-produces-outer-membrane-vesicles-in-a-spatially-distinct-manner-related-to-capsular-polysaccharide
#17
Cheri M Hampton, Ricardo C Guerrero-Ferreira, Rachel E Storms, Jeannette V Taylor, Hong Yi, Paul A Gulig, Elizabeth R Wright
Vibrio vulnificus, a bacterial species that inhabits brackish waters, is an opportunistic pathogen of humans. V. vulnificus infections can cause acute gastroenteritis, invasive septicemia, tissue necrosis, and potentially death. Virulence factors associated with V. vulnificus include the capsular polysaccharide (CPS), lipopolysaccharide, flagellum, pili, and outer membrane vesicles (OMVs). The aims of this study were to characterize the morphology of V. vulnificus cells and the formation and arrangement of OMVs using cryo-electron microscopy (cryo-EM)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163381/functional-characterization-of-acinetobacter-baumannii-lacking-the-rna-chaperone-hfq
#18
Han-Yueh Kuo, Hsuan-Hao Chao, Po-Cheng Liao, Long Hsu, Kai-Chih Chang, Chi-Hua Tung, Chang-Hua Chen, Ming-Li Liou
The RNA chaperone Hfq is involved in the riboregulation of diverse genes via small RNAs. Recent studies have demonstrated that Hfq contributes to the stress response and the virulence of several pathogens, and the roles of Hfq vary among bacterial species. Here, we attempted to elucidate the role of Hfq in Acinetobacter baumannii ATCC 17978. In the absence of hfq, A. baumannii exhibited retarded cell growth and was highly sensitive to environmental stress, including osmotic and oxidative pressure, pH, and temperature...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29152576/meningococcal-antigen-typing-system-mats-based-neisseria-meningitidis-serogroup-b-coverage-prediction-for-the-menb-4c-vaccine-in-the-united-states
#19
Gowrisankar Rajam, Maria Stella, Ellie Kim, Simon Paulos, Giuseppe Boccadifuoco, Laura Serino, George Carlone, Duccio Medini
Neisseria meningitidis is the most common cause of bacterial meningitis in children and young adults worldwide. A 4-component vaccine against N. meningitidis serogroup B (MenB) disease (MenB-4C [Bexsero]; GSK) combining factor H binding protein (fHBP), neisserial heparin binding protein (NHBA), neisserial adhesin A (NadA), and PorA-containing outer membrane vesicles was recently approved for use in the United States and other countries worldwide. Because the public health impact of MenB-4C in the United States is unclear, we used the meningococcal antigen typing system (MATS) to assess the strain coverage in a panel of strains representative of serogroup B (NmB) disease in the United States...
November 2017: MSphere
https://www.readbyqxmd.com/read/29138318/snare-priming-is-essential-for-maturation-of-autophagosomes-but-not-for-their-formation
#20
Adi Abada, Smadar Levin-Zaidman, Ziv Porat, Tali Dadosh, Zvulun Elazar
Autophagy, a unique intracellular membrane-trafficking pathway, is initiated by the formation of an isolation membrane (phagophore) that engulfs cytoplasmic constituents, leading to generation of the autophagosome, a double-membrane vesicle, which is targeted to the lysosome. The outer autophagosomal membrane consequently fuses with the lysosomal membrane. Multiple membrane-fusion events mediated by SNARE molecules have been postulated to promote autophagy. αSNAP, the adaptor molecule for the SNARE-priming enzyme N-ethylmaleimide-sensitive factor (NSF) is known to be crucial for intracellular membrane fusion processes, but its role in autophagy remains unclear...
November 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
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