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Outer membrane vesicles

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https://www.readbyqxmd.com/read/28803609/comic-the-hidden-dynamics-of-mitochondrial-inner-compartments
#1
Bongki Cho, Woong Sun
Mitochondria have evolutionarily, functionally and structurally distinct outer- (OMM) and inner-membranes (IMM). Thus, mitochondrial morphology is controlled by independent but coordinated activity of fission and fusion of the OMM and IMM. Constriction and division of the OMM are mediated by endocytosis-like machineries, which include dynamin-related protein 1 with additional cytosolic vesicle scissoring machineries such as actin filament and Dynamin 2. However, structural alteration of the IMM during mitochondrial division has been poorly understood...
August 14, 2017: BMB Reports
https://www.readbyqxmd.com/read/28794055/effect-of-vaccine-elicited-antibodies-on-colonization-of-neisseria-meningitidis-serogroup-b-and-c-strains-in-a-human-bronchial-epithelial-cell-culture-model
#2
Vianca Vianzon, Beate Illek, Gregory R Moe
Capsular polysaccharide-protein conjugate vaccines protect individuals from invasive disease and decrease carriage, which reduces spread of the organism in the population. In contrast, antibodies elicited by plain polysaccharide or protein antigen-based meningococcal (Men) vaccines have little or no effect on decreasing carriage. In this study, we investigated the mechanism by which vaccine-induced human IgG antibodies affect colonization by serogroup B (MenB) or C (MenC) strains using a human bronchial epithelial cell culture model (16HBE14o-)...
August 9, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28790210/membrane-distribution-of-the-pseudomonas-quinolone-signal-modulates-outer-membrane-vesicle-production-in-pseudomonas%C3%A2-aeruginosa
#3
Catalina Florez, Julie E Raab, Adam C Cooke, Jeffrey W Schertzer
The Pseudomonas quinolone signal (PQS) is an important quorum-sensing molecule in Pseudomonas aeruginosa that also mediates its own packaging and transport by stimulating outer membrane vesicle (OMV) formation. Because OMVs have been implicated in many virulence-associated behaviors, it is critical that we understand how they are formed. Our group proposed the bilayer-couple model for OMV biogenesis, where PQS intercalates into the outer membrane, causing expansion of the outer leaflet and consequently inducing curvature...
August 8, 2017: MBio
https://www.readbyqxmd.com/read/28776689/charmm-gui-martini-maker-for-modeling-and-simulation-of-complex-bacterial-membranes-with-lipopolysaccharides
#4
Pin-Chia Hsu, Bart M H Bruininks, Damien Jefferies, Paulo Cesar Telles de Souza, Jumin Lee, Dhilon S Patel, Siewert J Marrink, Yifei Qi, Syma Khalid, Wonpil Im
A complex cell envelope, composed of a mixture of lipid types including lipopolysaccharides, protects bacteria from the external environment. Clearly, the proteins embedded within the various components of the cell envelope have an intricate relationship with their local environment. Therefore, to obtain meaningful results, molecular simulations need to mimic as far as possible this chemically heterogeneous system. However, setting up such systems for computational studies is far from trivial, and consequently the vast majority of simulations of outer membrane proteins still rely on oversimplified phospholipid membrane models...
August 3, 2017: Journal of Computational Chemistry
https://www.readbyqxmd.com/read/28770026/neisseria-lactamica-y92-1009-complete-genome-sequence
#5
Anish K Pandey, David W Cleary, Jay R Laver, Martin C J Maiden, Xavier Didelot, Andrew Gorringe, Robert C Read
We present the high quality, complete genome assembly of Neisseria lactamica Y92-1009 used to manufacture an outer membrane vesicle (OMV)-based vaccine, and a member of the Neisseria genus. The strain is available on request from the Public Health England Meningococcal Reference Unit. This Gram negative, dipplococcoid bacterium is an organism of worldwide clinical interest because human nasopharyngeal carriage is related inversely to the incidence of meningococcal disease, caused by Neisseria meningitidis. The organism sequenced was isolated during a school carriage survey in Northern Ireland in 1992 and has been the subject of a variety of laboratory and clinical studies...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28768669/immunogenicity-of-nontypeable-haemophilus-influenzae-outer-membrane-vesicles-and-protective-ability-in-the-chinchilla-model-of-otitis-media
#6
Linda E Winter, Stephen J Barenkamp
Outer membrane vesicles (OMVs) produced by Gram-negative bacteria are enriched in several outer membrane components, including major and minor outer membrane proteins and lipooligosaccharide. We assessed the functional activity of nontypeable Haemophilus influenzae (NTHi) OMV-specific antisera and the protective ability of NTHi OMVs as vaccine antigens in the chinchilla otitis media model. OMVs were purified from three HMW1/HMW2-expressing NTHi strains, two of which were also engineered to overexpress Hia proteins...
August 2, 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/28765539/bacterial-membrane-vesicles-transport-their-dna-cargo-into-host-cells
#7
Natalie J Bitto, Ross Chapman, Sacha Pidot, Adam Costin, Camden Lo, Jasmine Choi, Tanya D'Cruze, Eric C Reynolds, Stuart G Dashper, Lynne Turnbull, Cynthia B Whitchurch, Timothy P Stinear, Katryn J Stacey, Richard L Ferrero
Bacterial outer membrane vesicles (OMVs) are extracellular sacs containing biologically active products, such as proteins, cell wall components and toxins. OMVs are reported to contain DNA, however, little is known about the nature of this DNA, nor whether it can be transported into host cells. Our work demonstrates that chromosomal DNA is packaged into OMVs shed by bacteria during exponential phase. Most of this DNA was present on the external surfaces of OMVs, with smaller amounts located internally. The DNA within the internal compartments of Pseudomonas aeruginosa OMVs were consistently enriched in specific regions of the bacterial chromosome, encoding proteins involved in virulence, stress response, antibiotic resistance and metabolism...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28752705/-bacterial-outer-membrane-vesicles-as-nano-carriers-to-study-immunological-activities
#8
Chen Qi, W U Min, Bai Hongzhen, Guo Zeling, Zhou Jun, Wang Qingqing, Tang Guping
Objective: To prepare a nano-carrier based on combining bacterial outer membrane vesicles (OMV) with three block polymer pluronic F127 (PEO100-PPO65-PEO100) (OMV-F127) and to investigate its immunological activity. Methods: Attenuated salmonella (sal) was cultivated. OMV were separated by centrifugal ultrafiltration or ultrasonication, and OMV-F127 was prepared by mechanical extrudation method. The protein contents and compositions were tested with BCA and SDS-PAGE; the morphology of OMV, F127 and OMV-F127 were observed with FM and TEM; the particle sizes and their zeta potential were determined with DLS...
March 25, 2017: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
https://www.readbyqxmd.com/read/28747507/roles-of-cellular-nsf-protein-in-entry-and-nuclear-egress-of-budded-virions-of-autographa-californica-multiple-nucleopolyhedrovirus
#9
Ya Guo, Qi Yue, Jinli Gao, Zhe Wang, Yun-Ru Chen, Gary W Blissard, Tong-Xian Liu, Zhaofei Li
In eukaryotic cells, the soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) proteins comprise the minimal machinery that triggers fusion of transport vesicles with their target membranes. Comparative studies revealed that genes encoding the components of the SNARE system are highly conserved in yeast, insect, and human genomes. Upon infection of insect cells by the virus AcMNPV, the transcript levels of most SNARE genes were initially up-regulated. We found that overexpression of dominant-negative (DN) forms of NSF or knock-down of the expression of NSF, the key regulator of the SNARE system, significantly affected the infectious AcMNPV production...
July 26, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28745825/salmonella-choleraesuis-outer-membrane-vesicles-proteomics-and-immunogenicity
#10
Qiong Liu, Jie Yi, Kang Liang, Xiangmin Zhang, Qing Liu
Salmonella enterica serotype Choleraesuis (S. Choleraesuis), Gram-negative facultative intracellular pathogen is capable of inducing the cholera in pigs whose symptoms manifest as fever, depression, septicemia, arthritis, and diarrhea. Infections with S. Choleraesuis has resulted in great economic loss for the swine breeding operations. Bacterial outer membrane vesicles (OMVs) play an important role in pathogenicity and host-pathogen interaction. In this study, we purified OMVs released by S. Choleraesuis strain χ3545 and characterized their lipopolysaccharide (LPS) profile...
July 26, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28745311/outer-membrane-vesicle-associated-lipase-ftla-enhances-cellular-invasion-and-virulence-in-francisella-tularensis-lvs
#11
Fei Chen, Guolin Cui, Shuxia Wang, Manoj Kumar Mohan Nair, Lihong He, Xinyi Qi, Xiangmin Han, Hanqi Zhang, Jing-Ren Zhang, Jingliang Su
Francisella tularensis is a highly infectious intracellular pathogen that infects a wide range of host species and causes fatal pneumonic tularemia in humans. ftlA was identified as a potential virulence determinant of the F. tularensis live vaccine strain (LVS) in our previous transposon screen, but its function remained undefined. Here, we show that an unmarked deletion mutant of ftlA was avirulent in a pneumonia mouse model with a severely impaired capacity to infect host cells. Consistent with its sequence homology with GDSL lipase/esterase family proteins, the FtlA protein displayed lipolytic activity in both E...
July 26, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28744268/intestinal-anti-inflammatory-effects-of-outer-membrane-vesicles-from-escherichia-coli-nissle-1917-in-dss-experimental-colitis-in-mice
#12
María-José Fábrega, Alba Rodríguez-Nogales, José Garrido-Mesa, Francesca Algieri, Josefa Badía, Rosa Giménez, Julio Gálvez, Laura Baldomà
Escherichia coli Nissle 1917 (EcN) is a probiotic strain with proven efficacy in inducing and maintaining remission of ulcerative colitis. However, the microbial factors that mediate these beneficial effects are not fully known. Gram-negative bacteria release outer membrane vesicles (OMVs) as a direct pathway for delivering selected bacterial proteins and active compounds to the host. In fact, vesicles released by gut microbiota are emerging as key players in signaling processes in the intestinal mucosa. In the present study, the dextran sodium sulfate (DSS)-induced colitis mouse model was used to investigate the potential of EcN OMVs to ameliorate mucosal injury and inflammation in the gut...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28742972/stimuli-responsive-directional-vesicular-assembly-with-tunable-surface-functionality-and-impact-on-enzyme-inhibition
#13
Amrita Sikder, Debes Ray, Vinod K Aswal, Suhrit Ghosh
The article describes the self-assembly of a series of unsymmetrical bola-shaped π-amphiphiles (NDI-1, NDI-1a, NDI-2, NDI-3, and NDI-4) consisting of a hydrophobic naphthalene-diimide (NDI) chromophore attached to a nonionic hydrophilic wedge and an anionic headgroup in the two opposite arms of the central NDI. By design, only a single hydrazide group is linked either on the ionic or nonionic arm of the NDI. NDI-1 and NDI-1a are regioisomers differing only in the location of the hydrazide group, placed in the nonionic or ionic arm, respectively...
August 8, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28742866/contribution-of-factor-h-binding-protein-sequence-to-the-cross-reactivity-of-meningococcal-native-outer-membrane-vesicle-vaccines-with-over-expressed-fhbp-variant-group-1
#14
Arianna Marini, Omar Rossi, Maria Grazia Aruta, Francesca Micoli, Simona Rondini, Serafina Guadagnuolo, Isabel Delany, Ian R Henderson, Adam F Cunningham, Allan Saul, Calman A MacLennan, Oliver Koeberling
Factor H-binding protein (fHbp) is an important meningococcal vaccine antigen. Native outer membrane vesicles with over-expressed fHbp (NOMV OE fHbp) have been shown to induce antibodies with broader functional activity than recombinant fHbp (rfHbp). Improved understanding of this broad coverage would facilitate rational vaccine design. We performed a pair-wise analysis of 48 surface-exposed amino acids involved in interacting with factor H, among 383 fHbp variant group 1 sequences. We generated isogenic NOMV-producing meningococcal strains from an African serogroup W isolate, each over-expressing one of four fHbp variant group 1 sequences (ID 1, 5, 9, or 74), including those most common among invasive African meningococcal isolates...
2017: PloS One
https://www.readbyqxmd.com/read/28741379/tiny-rnas-and-their-voyage-via-extracellular-vesicles-secretion-of-bacterial-small-rna-and-eukaryotic-microrna
#15
Ji-Woong Choi, Jee-Hyun Um, Jin-Hyun Cho, Heon-Jin Lee
MicroRNAs are small non-coding RNAs that bind to the 3'-untranslated region of target mRNAs and have transcriptional or translational inhibitory function in eukaryotes. Before microRNAs were widely known, bacterial non-coding small RNAs around 50-200 nt in length were discovered whose mechanism of action resembled that of microRNAs. Recently, RNAs that are of similar size to or smaller than microRNAs have been discovered in bacteria and indeed, this class of small RNAs have been found throughout all domains of life...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28740179/legionella-pneumophila-infection-activates-bystander-cells-differentially-by-bacterial-and-host-cell-vesicles
#16
Anna Lena Jung, Christina Elena Herkt, Christine Schulz, Kathrin Bolte, Kerstin Seidel, Nicoletta Scheller, Alexandra Sittka-Stark, Wilhelm Bertrams, Bernd Schmeck
Extracellular vesicles from eukaryotic cells and outer membrane vesicles (OMVs) released from gram-negative bacteria have been described as mediators of pathogen-host interaction and intercellular communication. Legionella pneumophila (L. pneumophila) is a causative agent of severe pneumonia. The differential effect of bacterial and host cell vesicles in L. pneumophila infection is unknown so far. We infected THP-1-derived or primary human macrophages with L. pneumophila and isolated supernatant vesicles by differential centrifugation...
July 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28721849/effective-protection-of-mice-against-shigella-flexneri-with-a-new-self-adjuvant-multicomponent-vaccine
#17
Yadira Pastor, Ana Camacho, Ana Gloria Gil, Rocío Ramos, Adela López de Ceráin, Iván Peñuelas, Juan M Irache, Carlos Gamazo
PURPOSE: The aim of this study was to develop an immunogenic protective product against Shigella flexneri by employing a simple and safe heat treatment-based strategy. METHODOLOGY: The physicochemical characteristics of naturally produced (OMV) and heat-induced (HT) outer-membrane vesicles from S. flexneri were examined, including a comparison of the protein content of the products. Toxicological and biodistribution studies, and a preliminary experiment to examine the protective effectiveness of HT in a murine model of S...
July 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28717425/high-throughput-nanoparticle-tracking-analysis-for-monitoring-outer-membrane-vesicle-production
#18
Matthias J H Gerritzen, Dirk E Martens, René H Wijffels, Michiel Stork
Outer membrane vesicles (OMVs) are spherical membrane nanoparticles released by Gram-negative bacteria. OMVs can be quantified in complex matrices by nanoparticle tracking analysis (NTA). NTA can be performed in static mode or with continuous sample flow that results in analysis of more particles in a smaller time-frame. Flow measurements must be performed manually despite the availability of a sample changer on the NanoSight system. Here we present a method for automated measurements in flow mode. OMV quantification in flow mode results in lower variance in particle quantification (coefficient of variation (CV) of 6%, CV static measurements of 14%)...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28717032/th17-mediated-cross-protection-against-pneumococcal-carriage-by-vaccination-with-a-variable-antigen
#19
Kirsten Kuipers, Wouter S P Jong, Christa E van der Gaast-de Jongh, Diane Houben, Fred van Opzeeland, Elles Simonetti, Saskia van Selm, Ronald de Groot, Marije I Koenders, Taj Azarian, Elder Pupo, Peter van der Ley, Jeroen D Langereis, Aldert Zomer, Joen Luirink, Marien I de Jonge
Serotype-specific protection against a Streptococcus pneumoniae is an important limitation of the current polysaccharide-based vaccines. To prevent serotype replacement, reduce transmission and limit emergence of new variants, it is essential to induce broad protection and restrict pneumococcal colonization. In this study, we used a prototype vaccine formulation consisting of LPS-detoxified outer membrane vesicles (OMVs) from Salmonella Typhimurium displaying the variable N-terminus of PspA (α1α2) for intranasal vaccination, which induced strong Th17 immunity associated with substantial reduction of pneumococcal colonization...
July 17, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28705462/effectiveness-of-a-group-b-outer-membrane-vesicle-meningococcal-vaccine-against-gonorrhoea-in-new-zealand-a-retrospective-case-control-study
#20
Helen Petousis-Harris, Janine Paynter, Jane Morgan, Peter Saxton, Barbara McArdle, Felicity Goodyear-Smith, Steven Black
BACKGROUND: Gonorrhoea is a major global public health problem that is exacerbated by drug resistance. Effective vaccine development has been unsuccessful, but surveillance data suggest that outer membrane vesicle meningococcal group B vaccines affect the incidence of gonorrhoea. We assessed vaccine effectiveness of the outer membrane vesicle meningococcal B vaccine (MeNZB) against gonorrhoea in young adults aged 15-30 years in New Zealand. METHODS: We did a retrospective case-control study of patients at sexual health clinics aged 15-30 years who were born between Jan 1, 1984, and Dec 31, 1998, eligible to receive MeNZB, and diagnosed with gonorrhoea or chlamydia, or both...
July 7, 2017: Lancet
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