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https://www.readbyqxmd.com/read/29028118/elevated-serum-adipsin-may-predict-unsuccessful-treatment-for-cows-milk-allergy-but-other-biomarkers-do-not
#1
Susanna Salmivesi, Marita Paassilta, Heini Huhtala, Riina Nieminen, Eeva Moilanen, Matti Korppi
AIM: This study evaluated whether 15 allergy, immunology or inflammatory markers predicted the long-term use of cows' milk or milk products seven years after the start of oral immunotherapy (OIT) for cows' milk allergy in children. METHODS: The following laboratory parameters were measured before the OIT at Tampere University Hospital, Finland, and after the six-month escalation phase: serum total immunoglobulin (Ig) E, milk-specific IgG and IgG4, eosinophil cationic protein, eosinophil-derived neurotoxin, interleukins 4, 5, 6,10 and 12p70 and serum adipokines adiponectin, adipsin, leptin and resistin...
October 13, 2017: Acta Paediatrica
https://www.readbyqxmd.com/read/29026733/safety-of-a-new-synbiotic-starter-formula
#2
Yvan Vandenplas, Antonis Analitis, Chara Tziouvara, Athina Kountzoglou, Anastasia Drakou, Manos Tsouvalas, Antigoni Mavroudi, Ioannis Xinias
PURPOSE: Breastfeeding is the best way to feed all infants, but not all infants can be (exclusively) breastfed. Cow's milk based infant formula is the second choice infant feeding. METHODS: The safety of a new synbiotic infant formula, supplemented with Bifidobacterium lactis and fructo-oligosaccharides, with lactose and a whey/casein 60/40 protein ratio was tested in 280 infants during 3 months. RESULTS: The median age of the infants at inclusion was 0...
September 2017: Pediatric Gastroenterology, Hepatology & Nutrition
https://www.readbyqxmd.com/read/29025431/specific-ige-and-skin-prick-tests-to-diagnose-allergy-to-fresh-and-baked-cow-s-milk-according-to-age-a-systematic-review
#3
REVIEW
Barbara Cuomo, Giovanni Cosimo Indirli, Annamaria Bianchi, Stefania Arasi, Davide Caimmi, Arianna Dondi, Stefania La Grutta, Valentina Panetta, Maria Carmen Verga, Mauro Calvani
BACKGROUND: The diagnosis of IgE-mediated cow's milk allergy is often based on anamnesis, and on specific IgE (sIgE) levels and/or Skin Prick Tests (SPT), which have both a good sensitivity but a low specificity, often causing positive results in non-allergic subjects. Thus, oral food challenge is still the gold standard test for diagnosis, though being expensive, time-consuming and possibly at risk for severe allergic reactions. AIM: The aim of the present study was to perform a systematic review of the studies that have so far analyzed the positive predictive values for sIgE and SPT in the diagnosis of allergy to fresh and baked cow's milk according to age, and to identify possible cut-offs that may be useful in clinical practice...
October 12, 2017: Italian Journal of Pediatrics
https://www.readbyqxmd.com/read/29024972/effect-of-metabolic-status-on-conceptus-maternal-interactions-on-day-19-in-dairy-cattle-ii-effects-on-the-endometrial-transcriptome
#4
S Bauersachs, C A Simintiras, R G Sturmey, S Krebs, J Bick, H Blum, E Wolf, P Lonergan, N Forde
The aim of this study was to test the hypothesis that the metabolic stresses associated with lactation alter the ability of the endometrium to respond appropriately to the conceptus by examining endometrial gene expression on Day 19 of pregnancy. Immediately after calving, primiparous Holstein cows with similar production and fertility estimated breeding values (EBVs) were randomly divided into two groups and either dried off (i.e. never milked) immediately or milked twice daily. Approximately 65-75 days postpartum, grade 1 blastocysts recovered from superovulated Holstein heifer donors (n = 5) were transferred (1 per recipient) into lactating (n = 11) and non-lactating (n = 11) recipients...
August 31, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29024632/quantitative-proteome-analysis-of-bovine-mammary-gland-reveals-protein-dynamic-changes-involved-in-peak-and-late-lactation-stages
#5
Xianrui Zheng, Chao Ning, Yichun Dong, Pengju Zhao, Junhui Li, Ziyao Fan, Jiang Li, Ying Yu, Raphael Mrode, Jian-Feng Liu
Mammary gland is an important organ for milk synthesis and secretion. It undergoes dramatic physiological changes to adapt the shift from peak to late lactation stage. Protein plays a final very vital role in many life functions, and the protein changes during different lactation stages potentially reflect the biology of lactation and the functions of mammary gland in cows. In current study, we adopted tandem mass tags label-based quantitative analysis technique and to investigate proteome changes occurring in bovine mammary gland from peak to late lactation stages...
October 9, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29024131/the-effect-of-dietary-supplementation-with-aurantiochytrium-limacinum-on-lactating-dairy-cows-in-terms-of-animal-health-productivity-and-milk-composition
#6
C A Moran, M Morlacchini, J D Keegan, G Fusconi
The aim of this study was to investigate the effect of dietary supplementation with the docosahexaenoic acid (DHA)-rich microalgae, Aurantiochytrium limacinum (AURA), on a variety of health and productivity parameters in lactating cows. Twenty-four cows were blocked by parity and number of days in milk and then randomly assigned to a control (CON; n = 12) group with no algal supplementation, or a treatment group (AURA; n = 12) provided with 100 g AURA cow(-1)  day(-1) or 16 g DHA cow(-1)  day(-1) ...
October 10, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29021893/the-efficacy-of-oral-and-subcutaneous-antigen-specific-immunotherapy-in-murine-cow-s-milk-and-peanut-allergy-models
#7
Marlotte M Vonk, Laura Wagenaar, Raymond H H Pieters, Leon M J Knippels, Linette E M Willemsen, Joost J Smit, Betty C A M van Esch, Johan Garssen
BACKGROUND: Antigen-specific immunotherapy (AIT) is a promising therapeutic approach for both cow's milk allergy (CMA) and peanut allergy (PNA), but needs optimization in terms of efficacy and safety. AIM: Compare oral immunotherapy (OIT) and subcutaneous immunotherapy (SCIT) in murine models for CMA and PNA and determine the dose of allergen needed to effectively modify parameters of allergy. METHODS: Female C3H/HeOuJ mice were sensitized intragastrically (i...
2017: Clinical and Translational Allergy
https://www.readbyqxmd.com/read/29019855/bovine-colostrum-for-preterm-infants-in-the-first-days-of-life-a-randomized-controlled-pilot-trial
#8
Sandra Meinich Juhl, Xuqiang Ye, Ping Zhou, Yanqi Li, Elisabeth Omolabake Iyore, Lixia Zhang, Pingping Jiang, Johannes B van Goudoever, Gorm Greisen, Per Torp Sangild
OBJECTIVES: Mother's own milk (MM) is the best nutrition for newborn preterm infants, but is often limited in supply just after birth. Pasteurized human donor milk (DM), and especially preterm infant formula (IF) are less optimal diets than MM. We hypothesized that it is feasible to use bovine colostrum (BC), the first milk from cows, as a supplement to MM, during the first weeks after preterm birth. METHODS: In an open-label, randomized, controlled pilot safety trial, supplementation of MM with BC was compared with DM supplementation (Danish unit) or IF supplementation (Chinese unit)...
October 10, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/29018530/benchmarking-welfare-indicators-in-73-free-stall-dairy-farms-in-north-western-spain
#9
Yolanda Trillo, Luis Angel Quintela, Mónica Barrio, Juan José Becerra, Ana Isabel Peña, Marcos Vigo, Pedro Garcia Herradon
The aim of this study was to describe the status of body condition score (BCS), hock injuries prevalence, locomotion and body hygiene score as animal welfare measures in 73 free-stall dairy cattle farms in Lugo (Spain). A benchmarking process was established across farms: (1) the animal-based indicators were ordered from low to high values; (2) The farms were classified into three categories based on the number of indicators within less than the 25th percentile, 25th to 75th percentile and above the 75th percentile...
2017: Veterinary Record Open
https://www.readbyqxmd.com/read/29018412/a-novel-lactobacilli-based-teat-disinfectant-for-improving-bacterial-communities-in-the-milks-of-cow-teats-with-subclinical-mastitis
#10
Jie Yu, Yan Ren, XiaoXia Xi, Weiqiang Huang, Heping Zhang
Teat disinfection pre- and post-milking is important for the overall health and hygiene of dairy cows. The objective of this study was to evaluate the efficacy of a novel probiotic lactobacilli-based teat disinfectant based on changes in somatic cell count (SCC) and profiling of the bacterial community. A total of 69 raw milk samples were obtained from eleven Holstein-Friesian dairy cows over 12 days of teat dipping in China. Single molecule, real-time sequencing technology (SMRT) was employed to profile changes in the bacterial community during the cleaning protocol and to compare the efficacy of probiotic lactic acid bacteria (LAB) and commercial teat disinfectants...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29017902/small-percentage-of-anaphylactic-reactions-treated-with-epinephrine-during-food-challenges-in-dutch-children
#11
Johanna P M van der Valk, Irene Berends, Roy Gerth van Wijk, Nicolette J T Arends, Maurits S van Maaren, Hans de Groot, Harry J Wichers, Joyce A M Emons, Anthony E J Dubois, Nicolette W de Jong
BACKGROUND: Severe allergic reactions, including anaphylaxis, occur during oral food challenges (OFCs) and the first-line treatment of anaphylaxis is epinephrine. OBJECTIVE: To evaluate the percentage of anaphylactic reactions treated with epinephrine during OFCs and to identify associated factors for the administration of epinephrine. METHODS: Children who underwent an OFC with peanut, hazelnut, cow's milk, hen's egg, or cashew nut from 2005 through 2015 in the Netherlands were evaluated...
October 7, 2017: Annals of Allergy, Asthma & Immunology
https://www.readbyqxmd.com/read/29016916/sensitization-to-cow-s-milk-protein-in-a-dairy-worker
#12
B Quirantes Sierra, A Lara Jiménez, M Skodova
Background: Repeated skin exposure and inhalation of milk proteins may lead to IgE-mediated sensitization, normally manifested at the site of contact. Although this is a phenomenon predominantly found in children, it has also been observed in adults. Aims: To describe a case of a worker who, after being exposed to milk proteins for many years, eventually experienced episodes of anaphylaxis. Case report: A 62-year-old worker experienced severe episodes of anaphylaxis following the intake of dairy products...
September 2, 2017: Occupational Medicine
https://www.readbyqxmd.com/read/29016691/generation-of-beta-lactoglobulin-knock-out-goats-using-crispr-cas9
#13
Wenjun Zhou, Yongjie Wan, Rihong Guo, Mingtian Deng, Kaiping Deng, Zhen Wang, Yanli Zhang, Feng Wang
Goat's milk, considered a substitute for cow's milk, has a high nutritional value. However, goat's milk contains various allergens, predominantly β-lactoglobulin (BLG). In this study, we employed the CRISPR/Cas9 system to target the BLG locus in goat fibroblasts for sgRNA optimization and generate BLG knock-out goats through co-injection of Cas9 mRNA and small guide RNAs (sgRNAs) into goat embryos at the one-cell stage. We firstly tested sgRNA editing efficiencies in goat fibroblast cells, and approximately 8...
2017: PloS One
https://www.readbyqxmd.com/read/28994453/effects-of-two-different-domestic-boiling-practices-on-the-allergenicity-of-cow-milk-proteins
#14
Cristina Lamberti, Cristina Baro, Marzia Giribaldi, Lorenzo Napolitano, Laura Cavallarin, Maria Gabriella Giuffrida
BACKGROUND: the sale of raw drinking milk through automatic dispensers is permitted in some European Member States, but consumers are usually advised to boil the milk before consumption. The present study has been conducted to evaluate the effects of two common domestic boiling techniques on the proteins of raw milk, and, in particular, on their potential allergenicity. RESULTS: Native 1-D electrophoresis, N-terminal amino acid sequencing and immunoblotting have been used to characterize the protein pattern, and to evaluate the possible changes in the allergenic properties of the processed milk...
October 10, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28992929/antibiotic-use-by-farmers-to-control-mastitis-as-influenced-by-health-advice-and-dairy-farming-systems
#15
A Poizat, F Bonnet-Beaugrand, A Rault, C Fourichon, N Bareille
Mastitis is a bacterial disease common in dairy farms. Although knowledge about mastitis and its optimal technical management and treatment is now available, some dairy farmers still use antibiotics in inappropriate ways. Antibiotic use by farmers can be influenced by personal restraints and motivations, but it can be assumed that external drivers are also influential. The main purpose of this article is thus to analyse the choices of antibiotic and alternative medicine use for mastitis treatment and investigate the possible influence of two unexplored external drivers in dairy farms: (i) the health advice offered to farmers by farm advisors and veterinarians, (ii) the dairy farming system, as defined by combining the market valuation chosen for the milk, the level of intensification, and the perceived pressure related to investments...
October 1, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/28992916/association-of-clinical-signs-after-acute-schmallenberg-virus-infection-with-milk-production-and-fertility-in-swiss-dairy-cows
#16
Isabel Lechner, Marianne Wüthrich, Mireille Meylan, Bart H P van den Borne, Gertraud Schüpbach-Regula
Since its first occurrence in August 2011 in Germany and the Netherlands, the Schmallenberg virus (SBV) spread rapidly across Europe, where it caused production losses and abortions in ruminants as well as congenital malformations in the offspring of affected animals. Several studies have investigated the impact of SBV on fertility and production parameters in dairy cows at herd level. However, the impact of clinical disease at the animal level remained undetermined. This study aimed at estimating the impact of clinical disease during and after an infection with SBV on production and fertility parameters in individual Swiss dairy cows...
October 1, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/28992005/characterization-of-exosomes-from-body-fluids-of-dairy-cows
#17
Y Q Koh, H N Peiris, K Vaswani, S Meier, C R Burke, K A Macdonald, J R Roche, F Almughlliq, B J Arachchige, S Reed, M D Mitchell
Exosomes are a specific subpopulation of extracellular vesicles that are widely released by cells of different origins with divergent functions that make their way into body fluids that can be conveniently sampled. In the current study, we isolated and evaluated exosomes from concurrently collected samples of milk, plasma, saliva, and urine from a group of 6 pregnant Holstein-Friesian dairy cows (aged 7 mo, 174 to 203 d of gestation). The cows had BCS of 3.5 to 5.25 (on a scale of 1 to 10), and the milk production for the season to the time of sampling ranged between 5,118 and 6,959 kg...
September 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28990226/milk-fatty-acid-profile-from-cows-fed-with-mixed-rations-and-different-access-time-to-pastureland-during-early-lactation
#18
J Barca, M Carriquiry, L Olazabal, M Fajardo, P Chilibroste, A Meikle
Milk fatty acid (FA) profiles were determined in Holstein cows (n = 27) fed total mixed rations (TMR) ad libitum (G0) or diet composed by TMR (50% dry matter [DM] offered) plus grazing of pasture with 6 hr of access time to paddock in one session (G1) or 9 hr in two sessions (G2) at 45 days in milk (DIM). Moreover, milk FA was determined at 65 DIM when G0 cows turned out to G1 diet without adaptation period (Post-G0), G1 remained as controls. Milk FA was quantified using gas chromatography and mass spectrometry...
October 8, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28987717/protective-effect-of-rumenic-acid-rich-cow-s-milk-against-colitis-is-associated-with-the-activation-of-nrf2-pathway-in-a-murine-model
#19
P Bergamo, E Cocca, A Monaco, V Cozzolino, F Boscaino, I Ferrandino, F Maurano, M Rossi
Dietary supplementation with pure cis9, trans11 isomer of Conjugated Linoleic Acid -known as Rumenic Acid (RA)- improves cytoprotective defenses downstream through the activation of nuclear factor-E2-related factor-2(Nrf2). This capability, when Rumenic Acid is consumed in the form of foods, is still unknown. The ability of standard (St) or cow milk naturally-enriched in RA (En) to activate Nrf2 pathway and its impact on dextran sodium sulfate (DSS)-induced colitis was comparatively evaluated. Activity of Nrf2 pathway was investigated in colonic tissue of BALB/c mice, receiving 4-week supplement with skimmed milk (SK), St or St reinforced with pure RA (RSt) providing increasing RA dose (0, 124 or 404mg RA/kg(-1) b...
October 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
https://www.readbyqxmd.com/read/28987591/a-dynamic-model-to-simulate-potassium-balance-in-dairy-cows
#20
M Berg, J Plöntzke, S Leonhard-Marek, K E Müller, S Röblitz
High-performing dairy cows require a particular composition of nutritional ingredients, adapted to their individual requirements and depending on their production status. The optimal dimensioning of minerals in the diet, one being potassium, is indispensable for the prevention of imbalances. Potassium balance in cows is the result of potassium intake, distribution in the organism, and excretion, and it is closely related to glucose and electrolyte metabolism. In this paper, we present a dynamical model for potassium balance in lactating and nonlactating dairy cows based on ordinary differential equations...
October 4, 2017: Journal of Dairy Science
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