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https://www.readbyqxmd.com/read/28106476/quantification-of-hepcidin-25-in-human-cerebrospinal-fluid-using-lc-ms-ms
#1
Constance Delaby, Pauline Bros, Jérôme Vialaret, Amandine Moulinier, Vincent Delatour, Audrey Gabelle, Sylvain Lehmann, Christophe Hirtz
AIM: Hepcidin, the main iron metabolism regulator, can be detected in various biological fluids. Here, we describe a quantitative method of LC-MS/MS to quantify the 25 amino acid isoform of hepcidin (hepcidin-25) in human cerebrospinal fluid (CSF). Results & methodology: Samples were prepared through protein precipitation followed by solid phase extraction (SPE) and injected into a triple-quadrupole mass spectrometer. Validation of our method included determination of LOQ (0.1 ng/ml), repeatability, intermediate precision, recovery and linearity (up to 25 ng/ml)...
January 20, 2017: Bioanalysis
https://www.readbyqxmd.com/read/28103409/zea-mays-fe-deficiency-related-4-zmfdr4-functions-as-an-iron-transporter-in-the-plastids-of-monocots
#2
Xiu-Yue Zhang, Xi Zhang, Qi Zhang, Xiao-Xi Pan, Luo-Chen Yan, Xiao-Juan Ma, Wei-Zhong Zhao, Xiao-Ting Qi, Li-Ping Yin
Iron (Fe)-homeostasis in the plastids is closely associated with Fe transport proteins that prevent Fe from occurring in its toxic free ionic forms. However, the number of known protein families related to Fe transport in the plastids (about 5) and the function of iron in non-green plastids is limited. In the present study, we report the functional characterization of Zea mays Fe deficiency-related 4 (ZmFDR4), which was isolated from a differentially expressed clone of a cDNA library of Fe deficiency-induced maize roots...
January 19, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28058666/analysis-of-trace-elements-in-rat-bronchoalveolar-lavage-fluid-by-inductively-coupled-plasma-mass-spectrometry
#3
Wajhul Qamar, Abdul Rahman Al-Ghadeer, Raisuddin Ali, Hatem A Abuelizz
The main objective was to determine the elemental profile of the lung lining fluid of rats which are used as model animals in various experiments. Lung lining fluid elemental constitution obtained after bronchoalveolar lavage fluid (BALF) was analyzed by inductively coupled plasma mass spectrometry (ICP-MS) to determine the biological trace elements along with calcium and magnesium. BALF was collected from healthy rats using a tracheal cannula. However, cells in BALF were counted to monitor any underlying inflammatory lung condition...
January 5, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28050844/microbial-nitrate-removal-by-waste-iron-shavings-from-the-biological-and-catalytic-ozonation-treated-dyeing-and-finishing-wastewater
#4
Jieting Ma, Yunlu Chen, Gang Luo, Jianxin Nie, Zhigang Guo, Yan Liu, Luming Ma
The concentration of total nitrogen (TN) (between 40 and 60 mg/L, mainly nitrate) in the biological and catalytic ozonation treated dyeing and finishing wastewater needs to be reduced before discharge. The present study investigated the feasibility of using waste iron shavings as electron donor for nitrogen removal by biological denitrification. Two anoxic sequencing batch reactors (AnSBR) were continuously operated for more than 100 days. The results showed that the TN removal efficiency increased from 12% in the control reactor (AnSBR-C) to 20% in the reactor with waste iron shavings (AnSBR-Fe)...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28038965/inversion-recovery-ultrashort-echo-time-magnetic-resonance-imaging-a-method-for-simultaneous-direct-detection-of-myelin-and-high-signal-demonstration-of-iron-deposition-in-the-brain-a-feasibility-study
#5
Vipul R Sheth, Shujuan Fan, Qun He, Yajun Ma, Jacopo Annese, Robert Switzer, Jody Corey-Bloom, Graeme M Bydder, Jiang Du
Multiple sclerosis (MS) causes demyelinating lesions in the white matter and increased iron deposition in the subcortical gray matter. Myelin protons have an extremely short T2* (<1ms) and are not directly detected with conventional clinical magnetic resonance (MR) imaging sequences. Iron deposition also reduces T2*, leading to reduced signal on clinical sequences. In this study we tested the hypothesis that the inversion recovery ultrashort echo time (IR-UTE) pulse sequence can directly and simultaneously image myelin and iron deposition using a clinical 3T scanner...
December 28, 2016: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/28035604/decomplexation-efficiency-and-mechanism-of-cu-ii-edta-by-h2o2-coupled-internal-micro-electrolysis-process
#6
Dongfang Zhou, Yongyou Hu, Qian Guo, Weiguang Yuan, Jiefan Deng, Yapan Dang
Internal micro-electrolysis (IE) coupled with Fenton oxidation (IEF) was a very effective technology for copper (Cu)-ethylenediaminetetraacetic acid (EDTA) wastewater treatment. However, the mechanisms of Cu(2+) removal and EDTA degradation were scarce and lack persuasion in the IEF process. In this paper, the decomplexation and removal efficiency of Cu-EDTA and the corresponding mechanisms during the IEF process were investigated by batch test. An empirical equation and the oxidation reduction potential (ORP) index were proposed to flexibly control IE and the Fenton process, respectively...
December 29, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28011255/the-%C3%AE-parameter-of-anomalous-diffusion-quantified-in-human-brain-by-mri-depends-on-local-magnetic-susceptibility-differences
#7
A Caporale, M Palombo, E Macaluso, M Guerreri, M Bozzali, S Capuani
Motivated by previous results obtained in vitro, we investigated the dependence of the anomalous diffusion (AD) MRI technique on local magnetic susceptibility differences (Δχ) driven by magnetic field inhomogeneity in human brains. The AD-imaging contrast investigated here is quantified by the stretched-exponential parameter γ, extracted from diffusion weighted (DW) data collected by varying diffusion gradient strengths. We performed T2* and DW experiments in eight healthy subjects at 3.0T. T2*-weighted images at different TEs=(10,20,35,55)ms and DW-EPI images with fourteen b-values from 0 to 5000s/mm(2) were acquired...
December 21, 2016: NeuroImage
https://www.readbyqxmd.com/read/28010747/using-on-line-solid-phase-extraction-for-in%C3%A2-vivo-speciation-of-diffusible-ferrous-and-ferric-iron-in-living-rat-brain-extracellular-fluid
#8
Cheng-Kuan Su, Yi-Ting Chen, Yuh-Chang Sun
Exploration of brain extracellular non-protein-bound/diffusible iron species remains a critically important issue in investigations of free radical biology and neurodegenerative diseases. In this study, a facile sample pretreatment scheme, involving poly(vinyl chloride)-metal ion interactions as a selective extraction procedure, was optimized in conjunction with microdialysis (MD) sampling and inductively coupled plasma mass spectrometry (ICP-MS) in cool-plasma mode for in vivo online monitoring of rat brain extracellular Fe(II) and Fe(III) species...
February 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28007423/rgms-structural-insights-molecular-regulation-and-downstream-signaling
#9
REVIEW
Christian Siebold, Toshihide Yamashita, Philippe P Monnier, Bernhard K Mueller, R Jeroen Pasterkamp
Although originally discovered as neuronal growth cone-collapsing factors, repulsive guidance molecules (RGMs) are now known as key players in many fundamental processes, such as cell migration, differentiation, iron homeostasis, and apoptosis, during the development and homeostasis of many tissues and organs, including the nervous, skeletal, and immune systems. Furthermore, three RGMs (RGMa, RGMb/DRAGON, and RGMc/hemojuvelin) have been linked to the pathogenesis of various disorders ranging from multiple sclerosis (MS) to cancer and juvenile hemochromatosis (JHH)...
December 19, 2016: Trends in Cell Biology
https://www.readbyqxmd.com/read/27997280/betel-nut-chewing-in-iron-age-vietnam-detection-of-areca-catechu-alkaloids-in-dental-enamel
#10
Simone Krais, Miriam Klima, Laura M Huppertz, Volker Auwärter, Markus J Altenburger, Merja A Neukamm
The betel quid is one of the most commonly consumed psychoactive substances in the world. By archaeological evidence like the occurrence of areca nuts in archaeological sites, the typical overall reddish-brown staining on prehistoric human teeth or specific artifacts linked with the habit, it is assumed that this tradition reaches back to prehistoric times. Since this kind of evidence is indirect, it is frequently doubted. The present study provides the earliest direct analytical indication of betel nut chewing in human history...
December 20, 2016: Journal of Psychoactive Drugs
https://www.readbyqxmd.com/read/27981899/high-hydrostatic-pressure-assisted-enzymatic-treatment-improves-antioxidant-and-anti-inflammatory-properties-of-phosvitin
#11
Heejoo Yoo, Fatemeh Bamdad, Naiyana Gujral, Joo-Won Suh, Hoon Sunwoo
BACKGROUND: Phosvitin (PV) is a highly-phosphorylated metal-binding protein in egg yolk. Phosphoserine clusters make PV resistant to enzymatic digestion, which might be nutritionally undesirable. OBJECTIVE: This study was designed to determine the effects of high hydrostatic pressure and enzymatic hydrolysis (HHP-EH) on the antioxidant and anti-inflammatory properties of PV hydrolysates (PVHs). METHODS: PV was hydrolyzed by alcalase, elastase, savinase, thermolysin, and trypsin at 0...
December 16, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/27978916/-analysis-of-virulence-factors-of-porphyromonas-endodontalis-based-on-comparative-proteomics-technique
#12
H Li, H Ji, S S Wu, B X Hou
Objective: To analyze the protein expression profile and the potential virulence factors of Porphyromonas endodontalis (Pe) via comparison with that of two strains of Porphyromonas gingivalis (Pg) with high and low virulences, respectively. Methods: Whole cell comparative proteomics of Pe ATCC35406 was examined and compared with that of high virulent strain Pg W83 andlow virulent strain Pg ATCC33277, respectively. Isobaric tags for relative and absolute quantitation (iTRAQ) combined with nano liquid chromatography-tandem mass spectrometry (Nano-LC-MS/MS) were adopted to identify and quantitate the proteins of Pe and two strains of Pg with various virulences by using the methods of isotopically labeled peptides, mass spectrometric detection and bioinformatics analysis...
December 9, 2016: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/27976873/molecular-fractionation-of-dissolved-organic-matter-in-a-shallow-subterranean-estuary-the-role-of-the-iron-curtain
#13
Annika Linkhorst, Thorsten Dittmar, Hannelore Waska
Iron that precipitates under aerobic conditions in natural aquatic systems scavenges dissolved organic matter (DOM) from solution. Subterranean estuaries (STEs) are of major importance for land-ocean biogeochemical fluxes. Their specific redox boundaries, coined the "iron curtain" due to the abundance of precipitated iron(III) (oxy)hydroxides, are hot spots for the removal and redissolution of iron, associated nutrients, and DOM. We used ultra-high-resolution electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry to molecularly characterize the iron-coagulating fractions of 32 groundwater and seawater DOM samples along a salinity gradient from a shallow STE on Spiekeroog Island, North Sea, Germany, and linked our findings to trace metal and nutrient concentrations...
January 19, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/27943581/reverse-biosynthesis-generating-combinatorial-pools-of-drug-leads-from-enzyme-mediated-fragmentation-of-natural-products
#14
Tomas Richardson-Sanchez, William Tieu, Rachel Codd
A combinatorial pool of hydroxamic acid fragments as potential metalloprotein drug leads was generated from the enzyme-mediated hydrolysis of the natural product desferrioxamine B (DFOB). DFOB is a metabolite produced by Streptomyces pilosus for iron acquisition that can be selectively catabolized by Niveispirillum irakense to access carbon for growth. The supernatant of a DFOB-supplemented culture of N. irakense was analyzed by LC-MS at 7-8 intervals to 168 h and a mixture of endo-hydroxamic acid fragments were identified that contained reactive terminal groups...
December 11, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27936592/multicore-magnetic-nanoparticles-coated-with-oligomeric-micelles-characterization-and-potential-for-the-extraction-of-contaminants-over-a-wide-polarity-range
#15
Mohamed Naous, Diego García-Gómez, Francisco José López-Jiménez, Farida Bouanani, María Loreto Lunar, Soledad Rubio
Oligomeric micelles from sodium undecylenate (oSUD) were chemisorbed to magnetic iron oxide nanoparticles (MNPs) through a single-step synthetic route involving the simultaneous nanoparticle formation and functionalization in an aqueous medium. The resulting spherical nanoparticles (MNPs-oSUD) consisted of a concatenation of iron oxides cores, with an average size of 7.7 nm, bound by oSUD micelles (particle average diameter ca. 200 nm). Micellar coverage was around 50% by weight of the MNP-oSUD and offered multiple retention mechanisms (e...
December 12, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27933069/accumulation-and-secretion-of-coumarinolignans-and-other-coumarins-in-arabidopsis-thaliana-roots-in-response-to-iron-deficiency-at-high-ph
#16
Patricia Sisó-Terraza, Adrián Luis-Villarroya, Pierre Fourcroy, Jean-François Briat, Anunciación Abadía, Frédéric Gaymard, Javier Abadía, Ana Álvarez-Fernández
Root secretion of coumarin-phenolic type compounds has been recently shown to be related to Arabidopsis thaliana tolerance to Fe deficiency at high pH. Previous studies revealed the identity of a few simple coumarins occurring in roots and exudates of Fe-deficient A. thaliana plants, and left open the possible existence of other unknown phenolics. We used HPLC-UV/VIS/ESI-MS(TOF), HPLC/ESI-MS(ion trap) and HPLC/ESI-MS(Q-TOF) to characterize (identify and quantify) phenolic-type compounds accumulated in roots or secreted into the nutrient solution of A...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27931173/cosmetic-wastewater-treatment-by-the-zvi-h2o2-process
#17
Jan Bogacki, Piotr Marcinowski, Ewa Zapałowska, Justyna Maksymiec, Jeremi Naumczyk
The ZVI/H2O2 process was applied for cosmetic wastewater treatment. Two commercial zero-valent iron (ZVI) types with different granulations were chosen: Hepure Ferrox PRB and Hepure Ferrox Target. In addition, the pH and stirring method influence on ZVI/H2O2 process efficiency was studied. During the ZVI and ZVI/H2O2 processes, linear Fe ions concentration increase was observed. The addition of H2O2 significantly accelerated the iron dissolution process. The highest COD removal was obtained using finer ZVI (Hepure Ferrox Target) for doses of reagents ZVI/H2O2 1500/1600 mg/L, in a H2O2/COD weight ratio 2:1, at pH 3...
December 28, 2016: Environmental Technology
https://www.readbyqxmd.com/read/27908408/protective-effect-of-magnesium-acetyltaurate-against-nmda-induced-retinal-damage-involves-restoration-of-minerals-and-trace-elements-homeostasis
#18
Azliana Jusnida Ahmad Jafri, Natasha Najwa Nor Arfuzir, Lidawani Lambuk, Igor Iezhitsa, Renu Agarwal, Puneet Agarwal, Norhafiza Razali, Anna Krasilnikova, Maria Kharitonova, Vasily Demidov, Evgeny Serebryansky, Anatoly Skalny, Alexander Spasov, Ahmad Pauzi Md Yusof, Nafeeza Mohd Ismail
Glutamate-mediated excitotoxicity involving N-methyl-d-aspartate (NMDA) receptors has been recognized as a final common outcome in pathological conditions involving death of retinal ganglion cells (RGCs). Overstimulation of NMDA receptors results in influx of calcium (Ca) and sodium (Na) ions and efflux of potassium (K). NMDA receptors are blocked by magnesium (Mg). Such changes due to NMDA overstimulation are also associated with not only the altered levels of minerals but also that of trace elements and redox status...
January 2017: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/27906525/the-clinical-utility-of-qsm-disease-diagnosis-medical-management-and-surgical-planning
#19
REVIEW
Sarah Eskreis-Winkler, Yan Zhang, Jingwei Zhang, Zhe Liu, Alexey Dimov, Ajay Gupta, Yi Wang
Quantitative susceptibility mapping (QSM) is an MR technique that depicts and quantifies magnetic susceptibility sources. Mapping iron, the dominant susceptibility source in the brain, has many important clinical applications. Herein, we review QSM applications in the diagnosis, medical management, and surgical treatment of disease. To assist in early disease diagnosis, QSM can identify elevated iron levels in the motor cortex of amyotrophic lateral sclerosis patients, in the substantia nigra of Parkinson's disease (PD) patients, in the globus pallidus, putamen, and caudate of Huntington's disease patients, and in the basal ganglia of Wilson's disease patients...
December 1, 2016: NMR in Biomedicine
https://www.readbyqxmd.com/read/27905613/unambiguous-identification-and-discovery-of-bacterial-siderophores-by-direct-injection-21-tesla-fourier-transform-ion-cyclotron-resonance-mass-spectrometry
#20
Lawrence R Walker, Malak M Tfaily, Jared B Shaw, Nancy J Hess, Ljiljana Paša-Tolić, David W Koppenaal
Under iron-limiting conditions, bacteria produce low molecular mass Fe(iii) binding molecules known as siderophores to sequester the Fe(iii), along with other elements, increasing their bioavailability. Siderophores are thought to influence iron cycling and biogeochemistry in both marine and terrestrial ecosystems and hence the need for rapid, confident characterization of these compounds has increased. In this study, the type of siderophores produced by two marine bacterial species, Synechococcus sp. PCC 7002 and Vibrio cyclitrophicus 1F53, were characterized by use of a newly developed 21 T Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (FTICR MS) with direct injection electrospray ionization...
December 1, 2016: Metallomics: Integrated Biometal Science
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