keyword
MENU ▼
Read by QxMD icon Read
search

Molecule diagnosis

keyword
https://www.readbyqxmd.com/read/29147508/chiral-nanoprobes-for-targeting-and-long-term-imaging-of-the-golgi-apparatus
#1
Rong Sheng Li, Peng Fei Gao, Hong Zhi Zhang, Lin Ling Zheng, Chun Mei Li, Jian Wang, Yuan Fang Li, Feng Liu, Na Li, Cheng Zhi Huang
The Golgi apparatus is an essential subcellular organelle. Targeting and monitoring the Golgi change at the single-cell level over a long time scale are critical but are challenges that have not yet been tackled. Inspired by the precise Golgi positioning ability of galactosyltransferase and protein kinase D, due to their cysteine residues, we developed a method for long-term Golgi imaging. Fluorescent molecules, carbon quantum dots (CQDs) and silica nanoparticles could target the Golgi when they are modified with l-cysteine...
October 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/29146581/long-noncoding-rnas-emerging-players-in-thyroid-cancer-pathogenesis
#2
Avaniyapuram Kannan Murugan, Munirajan Arasambattu Kannan, Ali S Alzahrani
Thyroid cancer continues to be the most common malignancy of endocrine glands. The incidence of thyroid cancer rises significantly over the past 4 decades and emerges as a major health issue. In recent years, a significant progress has been achieved in our understanding of molecular mechanisms of thyroid carcinogenesis, resulting in significant diagnostic, prognostic and therapeutic implications; yet, it has not reached a satisfactory level. Identifying novel molecular therapeutic targets and molecules for diagnosis and prognosis are expected to advance the overall management of this common malignancy...
November 16, 2017: Endocrine-related Cancer
https://www.readbyqxmd.com/read/29144729/an-optical-nanoantenna-for-single-molecule-based-detection-of-zika-virus-nucleic-acids-without-molecular-multiplication
#3
Sarah Elisabeth Ochmann, Carolin Vietz, Kateryna Trofymchuk, Guillermo P Acuna, Birka Lalkens, Philip Tinnefeld
Due to the limited signal-to-background ratio, molecular diagnostics requires molecular amplification of the target molecules or molecular signal amplification after target recognition. For direct molecular detection, we demonstrate a purely physical fluorescence enhancement process which can elevate the fluorescence signal of single fluorescent dyes by several orders of magnitude. To this end, DNA origami-based optical antennas with a height of around 125 nm are used, which utilize metallic nanoparticles to create a hotspot where fluorescence signals are enhanced by plasmonic effects...
November 16, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29144726/physalis-mottle-virus-like-particles-as-nanocarriers-for-imaging-reagents-and-drugs
#4
Hema Masarapu, Bindi K Patel, Paul L Chariou, He Hu, Neetu M Gulati, Bradley L Carpenter, Reza A Ghiladi, Sourabh Shukla, Nicole F Steinmetz
Platform technologies based on plant virus nanoparticles (VNPs) and virus-like particles (VLPs) are attracting the attention of researchers and clinicians because the particles are biocompatible, biodegradable, noninfectious in mammals, and can readily be chemically and genetically engineered to carry imaging agents and drugs. When the Physalis mottle virus (PhMV) coat protein is expressed in Escherichia coli, the resulting VLPs are nearly identical to the viruses formed in vivo. Here, we isolated PhMV-derived VLPs from ClearColi cells and carried out external and internal surface modification with fluorophores using reactive lysine-N-hydroxysuccinimide ester and cysteine-maleimide chemistries, respectively...
November 16, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/29143447/investigation-of-the-interactions-between-aptamer-and-misfolded-proteins-from-monomer-and-oligomer-to-fibril-by-single-molecule-force-spectroscopy
#5
Yan Zheng, Qing Wang, Xiaohai Yang, Zhiping Li, Lei Gao, Hua Zhang, Wenyan Nie, Xiuhua Geng, Kemin Wang
Increasing knowledge on the understanding interactions of aptamer with misfolded proteins (including monomer, oligomer, and amyloid fibril) is crucial for development of aggregation inhibitors and diagnosis of amyloid diseases. Herein, the interactions of lysozyme monomer-, oligomer-, and amyloid fibril-aptamer were investigated using single-molecule force spectroscopy. The results revealed that the aptamer screened against lysozyme monomer could also bind to oligomer and amyloid fibril, in spite of the recognition at a lower binding probability...
November 16, 2017: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/29142596/role-and-underlying-mechanisms-of-the-interstitial-protein-periostin-in-the-diagnosis-and-treatment-of-malignant-tumors
#6
Dong Ye, Zhi Sen Shen, Shi Jie Qiu, Qun Li, Guo Li Wang
Invasion and metastasis are the major characteristics of malignant tumors and are complex processes involving multiple genes. Gene regulation is a precise, large and complex biological control system, and its underlying mechanisms remain to be elucidated. Mesenchymal-specific genes are expressed primarily by mesenchymal cells, and the expression products of these genes are molecules with various structures and functions, including secreted proteins and extracellular matrix proteins. The periostin gene has been newly identified as a mesenchymal-specific gene and an extracellular-matrix secreted protein...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29140482/estimating-autoantibody-signatures-to-detect-autoimmune-disease-patient-subsets
#7
Zhenke Wu, Livia Casciola-Rosen, Ami A Shah, Antony Rosen, Scott L Zeger
Autoimmune diseases are characterized by highly specific immune responses against molecules in self-tissues. Different autoimmune diseases are characterized by distinct immune responses, making autoantibodies useful for diagnosis and prediction. In many diseases, the targets of autoantibodies are incompletely defined. Although the technologies for autoantibody discovery have advanced dramatically over the past decade, each of these techniques generates hundreds of possibilities, which are onerous and expensive to validate...
November 13, 2017: Biostatistics
https://www.readbyqxmd.com/read/29140403/klhl6-is-preferentially-expressed-in-germinal-center-derived-b-cell-lymphomas
#8
Christian A Kunder, Giovanna Roncador, Ranjana H Advani, Gabriela Gualco, Carlos E Bacchi, Jean M Sabile, Izidore S Lossos, Kexin Nie, Robert John Tibshirani, Michael R Green, Ash A Alizadeh, Yasodha Natkunam
Objectives: KLHL6 is a recently described BTB-Kelch protein with selective expression in lymphoid tissues and is most strongly expressed in germinal center B cells. Methods: Using gene expression profiling as well as immunohistochemistry with an anti-KLHL6 monoclonal antibody, we have characterized the expression of this molecule in normal and neoplastic tissues. Protein expression was evaluated in 1,058 hematopoietic neoplasms. Results: Consistent with its discovery as a germinal center marker, KLHL6 was positive mainly in B-cell neoplasms of germinal center derivation, including 95% of follicular lymphomas (106/112)...
November 11, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29138804/cucurbitacin-b-and-cancer-intervention-chemistry-biology-and-mechanisms-review
#9
Sukant Garg, Sunil C Kaul, Renu Wadhwa
Cancer is one of the most important healthcare matters, with the worst prognosis but the best possibilities for scientific development. It is likely to increase in the future and cause global havoc designating it as an epidemic. Cancer development requires urgent intervention. Past few decades have witnessed extensive research to challenge carcinogenesis. Treatment involving synthetic discipline is often associated with severe adverse effects, or even worsened prognosis. Accordingly, newer economic and patient friendly molecules are warranted...
November 10, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29137858/human-papillomavirus-e6-and-e7-oncoproteins-affect-the-cell-microenvironment-by-classical-secretion-and-extracellular-vesicles-delivery-of-inflammatory-mediators
#10
Marco Iuliano, Giorgio Mangino, Maria Vincenza Chiantore, Maria Simona Zangrillo, Rosita Accardi, Massimo Tommasino, Gianna Fiorucci, Giovanna Romeo
The connection between chronic inflammation and risk of cancer has been supported by several studies. The development of cancer might be a process driven by the presence of a specific combination of inflammatory mediators, including cytokines, chemokines and enzymes, in the tumor microenvironment. Virus-induced tumors, like HPV-induced Squamous Cell Carcinomas, represent a paradigmatic example of the interplay between inflammation, as integral part of the innate antiviral response, and malignant transformation...
November 11, 2017: Cytokine
https://www.readbyqxmd.com/read/29137675/human-hyaluronic-acid-synthase-1-promotes-malignant-transformation-via-epithelial-to-mesenchymal-transition-micronucleation-and-centrosome-abnormalities
#11
Nguyet Nguyen, Awanit Kumar, Simi Chacko, Rodney J Ouellette, Anirban Ghosh
BACKGROUND: Human hyaluronic acid (HA) molecules are synthesized by three membrane spanning Hyaluronic Acid Synthases (HAS1, HAS2 and HAS3). Of the three, HAS1 is found to be localized more into the cytoplasmic space where it synthesizes intracellular HA. HA is a ubiquitous glycosaminoglycan, mainly present in the extracellular matrix (ECM) and on the cell surface, but are also detected intracellularly. Accumulation of HA in cancer cells, the cancer-surrounding stroma, and ECM is generally considered an independent prognostic factors for patients...
November 14, 2017: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/29136911/one-step-detection-for-two-serological-biomarker-species-to-improve-the-diagnostic-accuracy-of-hepatocellular-carcinoma
#12
Tao Gao, Jun Zhi, Chaoli Mu, Shiyu Gu, Jing Xiao, Jie Yang, Zhaoxia Wang, Yang Xiang
At present, the accuracy of clinical hepatocellular carcinoma (HCC) diagnosis needs to be further improved. In this work, two kinds of serological biomarker species, microRNA and protein biomarker, have been detected simultaneously to identify HCC. Herein, a dual-aptamer hairpin DNA oligonucleotide is designed as the electrochemical sensing probe (ESP) to achieve this goal. The hairpin-structured DNA probe consists microRNA-16 (miR-16) complementary sequence and alpha fetoprotein (AFP) aptamer sequence, so it can both capture miR-16 and AFP...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29135009/bacterial-species-identifiable-magnetic-nanosystems-for-early-sepsis-diagnosis-and-extracorporeal-photodynamic-blood-disinfection
#13
Jianhao Wang, Hao Wu, Yanmei Yang, Rong Yan, Yuan Zhao, Yanhao Wang, Aihong Chen, Shilong Shao, Pengju Jiang, Yong-Qiang Li
Despite the numerous bacteria detection and elimination techniques available nowadays, sensitive diagnosis and treatment of sepsis (caused by the presence of bacteria in the bloodstream), especially at the early stage, remain big challenges. Here we report a nanosystem for early sepsis diagnosis and complete extracorporeal blood disinfection, based on iron oxide magnetic nanoparticles functionalized with chlorin e6 molecules and bacterial species-identifiable aptamers (Fe3O4-Ce6-Apt). We demonstrate that the Fe3O4-Ce6-Apt nanosystem can achieve simultaneous blood bacterial species identification and enrichment in a single step, and the enriched bacteria can be easily detected with the assistance of fluorescence microscopic determination...
November 14, 2017: Nanoscale
https://www.readbyqxmd.com/read/29132694/unrelated-immunodeficiency-states-may-impact-outcomes-and-immune-checkpoint-molecule-expression-in-patients-with-mycosis-fungoides-a-clinicopathologic-case-control-study
#14
Shay Warren, Meenal Kheterpal, Patricia L Myskowski, Alison Moskowitz, Steven M Horwitz, Melissa P Pulitzer
BACKGROUND: Immunodeficiency (ID) correlates with worse outcomes and decreased immune checkpoint molecule expression in melanoma. The impact of ID in mycosis fungoides (MF) is unknown. OBJECTIVE: Our goal was to evaluate the impact of ID in MF. METHODS: We conducted a case-control study of 17 patients with MF and ID versus age-, stage-, and race-matched controls as a subset of a comparative analysis of 23 patients with MF with ID (prior lymphoma, recent/current pregnancy, HIV, hypogammaglobulinemia, and prior chemotherapy) versus without ID...
November 10, 2017: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/29128425/using-sweat-to-measure-cytokines-in-older-adults-compared-to-younger-adults-a-pilot-study
#15
Melissa Hladek, Sarah L Szanton, Young-Eun Cho, Chen Lai, Caroline Sacko, Laken Roberts, Jessica Gill
BACKGROUND/OBJECTIVES: Current measures of cytokines involve urine, blood or saliva which have drawbacks including circadian rhythm variations and complicated collection methods. Sweat has been used to measure cytokines in young and middle-aged adults, but not older adults. We sought to determine the feasibility of using sweat to measure cytokines in older adults compared to younger adults. DESIGN: Two visit cross-sectional pilot study stratified by age group. SETTING: Independent living facility and Johns Hopkins University both in Maryland...
November 8, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29128078/applications-of-mass-cytometry-in-clinical-medicine-the-promise-and-perils-of-clinical-cytof
#16
REVIEW
Gregory K Behbehani
Mass cytometry is a novel technology similar to flow cytometry in which antibodies are tagged with heavy metal molecules rather than fluorophores and then detected with time-of-flight mass spectrometry. This enables measurement of up to 50 simultaneous parameters with no autofluorescent background and little or no spillover or required compensation. Mass cytometry has tremendous potential for the analysis of highly complex clinical samples for the diagnosis and monitoring of malignant and autoimmune disorders...
December 2017: Clinics in Laboratory Medicine
https://www.readbyqxmd.com/read/29125888/circular-rna-hsa_circ_0003221-circptk2-promotes-the-proliferation-and-migration-of-bladder-cancer-cells
#17
Zhen-Qiang Xu, Ming-Gen Yang, Hong-Jie Liu, Chen-Qiang Su
Emerging studies have shown that circular RNAs could be ideal biomarkers and even potential therapeutic targets for some tumors, including bladder cancer. However, only a few studies have investigated the circular RNAs in human bladder cancer. The key circular RNA molecules are closely related to bladder cancer and their roles remain largely unknown. Here, we investigated a novel circular RNA molecule, hsa-circ-0003221(circPTK2), which is differentially expressed in bladder carcinoma. Significant differential expression levels of circPTK2 were confirmed with quantitative PCR in 40 pairs of tissue and blood samples from patients with bladder carcinoma...
November 10, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29124837/synthetic-ion-channels-and-dna-logic-gates-as-components-of-molecular-robots
#18
Ryuji Kawano
A molecular robot is a next-generation biological robot consisting of biomaterials such as DNA, proteins, and lipids that imitates the actions of microorganisms. Three prerequisites have been proposed for the construction of such a robot: sensor, intelligence, and actuator. This minireview focuses on recent research on synthetic ion channels and DNA computing technologies, which are viewed as potential candidate components of molecular robots. Synthetic ion channels, which are embedded in an artificial cell membrane (lipid bilayer), sense ambient ions or chemicals and incorporate the molecules...
November 9, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/29124082/is-osteoporosis-an-autoimmune-mediated-disorder
#19
REVIEW
Rosebella A Iseme, Mark Mcevoy, Brian Kelly, Linda Agnew, Frederick R Walker, John Attia
The last two decades have marked a growing understanding of the interaction occurring between bone and immune cells. The chronic inflammation and immune system dysfunction commonly observed to occur during the ageing process and as part of a range of other pathological conditions, commonly associated with osteoporosis has led to the recognition of these processes as important determinants of bone disease. This is further supported by the recognition that the immune and bone systems in fact share regulatory mechanisms and progenitor molecules...
December 2017: Bone Reports
https://www.readbyqxmd.com/read/29123978/deep-sequencing-and-comprehensive-expression-analysis-identifies-several-molecules-potentially-related-to-human-poorly-differentiated-hepatocellular-carcinoma
#20
Ping Shao, Deguang Sun, Liming Wang, Rong Fan, Zhenming Gao
Hepatocellular carcinoma (HCC) that is graded histologically as poorly differentiated has a high recurrence, metastasis and poor prognosis. We sought to determine the regulatory mechanisms of HCC tumorigenesis and to identify molecules closely related to poorly differentiated HCC. High-throughput sequencing was used to construct microRNA (miRNA) and mRNA expression profiles for poorly differentiated HCC tissues and adjacent tissues. Network analysis was carried out to study miRNA-target interactions. Integrating the miRNA and mRNA data of HCC with four tumor grades from The Cancer Genome Atlas (TCGA) portal enabled the identification of potential closely related molecules for early diagnosis of poorly differentiated HCC...
November 2017: FEBS Open Bio
keyword
keyword
99073
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"