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https://www.readbyqxmd.com/read/29331900/a-catalytic-and-dual-recycling-amplification-atp-sensor-based-on-target-driven-allosteric-structure-switching-of-aptamer-beacons
#1
Ying Peng, Daxiu Li, Ruo Yuan, Yun Xiang
Abnormal concentrations of ATP are associated with many diseases and cancers, and quantitative detection of ATP is thus of great importance for disease diagnosis and prognosis. In the present work, we report a new dual recycling amplification sensor integrated with catalytic hairpin assembly (CHA) to achieve high sensitivity for fluorescent detection of ATP. The association of the target ATP with the aptamer beacons causes the allosteric structure switching of the aptamer beacons to expose the toehold regions, which hybridize with and unfold the fluorescently quenched hairpin signal probes (HP1) to recycle the target ATP and to trigger CHA between HP1 and the secondary hairpin probes (HP2) to form HP1/HP2 duplexes...
January 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29328390/investigation-into-the-underlying-molecular-mechanisms-of-hypertensive-nephrosclerosis-using-bioinformatics-analyses
#2
Tianlong Liu, Minna Liu, Peijin Shang, Xin Jin, Wenxing Liu, Yikai Zhang, Xinfang Li, Yi Ding, Yuwen Li, Aidong Wen
Hypertensive nephrosclerosis (HNS) is a major risk factor for end-stage renal disease. However, the underlying pathogenesis of HNS remains to be fully determined. The gene expression profile of GSE20602, which consists of 14 glomeruli samples from patients with HNS and 4 normal glomeruli control samples, was obtained from the Gene Expression Omnibus database. Gene ontology (GO) and pathway enrichment analyses were performed in order to investigate the functions and pathways of differentially expressed genes (DEGs)...
January 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29327123/text-mining-of-rheumatoid-arthritis-and-diabetes-mellitus-to-understand-the-mechanisms-of-chinese-medicine-in-different-diseases-with-same-treatment
#3
Ning Zhao, Guang Zheng, Jian Li, Hong-Yan Zhao, Cheng Lu, Miao Jiang, Chi Zhang, Hong-Tao Guo, Ai-Ping Lu
OBJECTIVE: To identify the commonalities between rheumatoid arthritis (RA) and diabetes mellitus (DM) to understand the mechanisms of Chinese medicine (CM) in different diseases with the same treatment. METHODS: A text mining approach was adopted to analyze the commonalities between RA and DM according to CM and biological elements. The major commonalities were subsequently verifified in RA and DM rat models, in which herbal formula for the treatment of both RA and DM identifified via text mining was used as the intervention...
January 9, 2018: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/29319258/familial-lecithin-cholesterol-acyl-transferase-deficiency-with-chronic-kidney-disease
#4
N Gopalakrishnan, R Arul, J Dhanapriya, T Dinesh Kumar, R Sakthirajan, T Balasubramaniyan
Familial lecithin-cholesterol acyltransferase (LCAT) deficiency is a rare autosomal recessive (AR) disease caused by mutation in the LCAT gene. LCAT enzyme esterifies cholesterol molecules in high-density lipoprotein(HDL) and low density-lipoprotein (LDL) particles. This enzyme deficiency is characterised by progressive corneal opacification, glomerulopathy, mild - moderate haemolytic anaemia and very low plasma levels of HDL. We here report a 34 year-old lady who presented with hypertension, nephrotic proteinuria, renal failure, corneal ring opacities, anemia and dyslipidemia...
October 2017: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/29315246/recent-advances-in-electrochemical-biosensors-based-on-enzyme-inhibition-for-clinical-and-pharmaceutical-applications
#5
REVIEW
Loubna El Harrad, Ilhame Bourais, Hasna Mohammadi, Aziz Amine
A large number of enzyme inhibitors are used as drugs to treat several diseases such as gout, diabetes, AIDS, depression, Parkinson's and Alzheimer's diseases. Electrochemical biosensors based on enzyme inhibition are useful devices for an easy, fast and environment friendly monitoring of inhibitors like drugs. In the last decades, electrochemical biosensors have shown great potentials in the detection of different drugs like neostigmine, ketoconazole, donepezil, allopurinol and many others. They attracted increasing attention due to the advantage of being high sensitive and accurate analytical tools, able to reach low detection limits and the possibility to be performed on real samples...
January 9, 2018: Sensors
https://www.readbyqxmd.com/read/29313945/endocrine-dysfunction-induced-by-immune-checkpoint-inhibitors-practical-recommendations-for-diagnosis-and-clinical-management
#6
REVIEW
Romualdo Barroso-Sousa, Patrick A Ott, F Stephen Hodi, Ursula B Kaiser, Sara M Tolaney, Le Min
Immune checkpoint inhibitors (ICIs) have revolutionized cancer therapy. However, because ICIs block coinhibitory molecules on T cells and other immune cells, unleashing them to mediate tumor cell killing, they also can disrupt the maintenance of immunological tolerance to self-antigens. Compared with chemotherapy, ICIs have a different toxicity profile, especially the occurrence of autoimmune-like manifestations against multiple organ systems, including endocrine glands, commonly referred to as immune-related adverse events...
January 3, 2018: Cancer
https://www.readbyqxmd.com/read/29313524/multichannel-spectrometers-in-animals
#7
Thomas Wells Cronin, Missael Garcia, Viktor Gruev
Multispectral, hyperspectral, polarimetric, and other types of multichannel imaging spectrometers are coming into common use for a variety of applications, including remote sensing, material identification, forensics, and medical diagnosis. These instruments are often bulky and intolerant of field abuse, so designing compact, reliable, portable, and robust devices is a priority. In contrast to most engineering designs, animals have been building compact and robust multichannel imaging systems for millennia - their eyes...
January 9, 2018: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/29313325/development-of-a-mirna-surface-enhanced-raman-scattering-assay-using-benchtop-and-handheld-raman-systems
#8
Monika Schechinger, Haley Marks, Andrea Locke, Mahua Choudhury, Gerard Cote
DNA-functionalized nanoparticles, when paired with surface-enhanced Raman spectroscopy (SERS), can rapidly detect microRNA. However, widespread use of this approach is hindered by drawbacks associated with large and expensive benchtop Raman microscopes. MicroRNA-17 (miRNA-17) has emerged as a potential epigenetic indicator of preeclampsia, a condition that occurs during pregnancy. Biomarker detection using an SERS point-of-care device could enable prompt diagnosis and prevention as early as the first trimester...
January 2018: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29311761/plasma-vascular-non-inflammatory-molecule-3-is-associated-with-gastrointestinal-acute-graft-versus-host-disease-in-mice
#9
Na Wang, Xiaoyi Qin, Yigeng Cao, Bin Liang, Kang Yu, Haige Ye
Background: Gastrointestinal acute graft-versus-host disease (GI aGVHD) is a lethal complication following allogeneic hematopoietic stem cell transplantation (HSCT). However, it is still very difficult to make a diagnosis of GI aGVHD in practice. To date, no consensus plasma biomarker of GI aGVHD can be used to help make a diagnosis. Here, we attempted to identify GI aGVHD associated plasma proteins in murine model, which can help make a diagnosis of GI aGVHD. Methods: We used 8-plex isobaric tags for relative and absolute quantitation (8-plex iTRAQ) to screen out proteins in plasma samples taken from murine models before and after allogeneic HSCT...
2018: Journal of Inflammation
https://www.readbyqxmd.com/read/29306436/activity-based-probes-for-glycosidases-profiling-and-other-applications
#10
Chi-Lin Kuo, Eline van Meel, Kassiani Kytidou, Wouter Willem Kallemeijn, Martin Witte, Herman Stephen Overkleeft, Marta Elena Artola, Johannes Maria Aerts
Glycosidases mediate the fragmentation of glycoconjugates in the body, including the vital recycling of endogenous molecules. Several inherited diseases in man concern deficiencies in lysosomal glycosidases degrading glycosphingolipids. Prominent is Gaucher disease caused by an impaired lysosomal β-glucosidase (glucocerebrosidase, GBA) and resulting in pathological lysosomal storage of glucosylceramide (glucocerebroside) in tissue macrophages. GBA is a retaining glucosidase with a characteristic glycosyl-enzyme intermediate formed during catalysis...
2018: Methods in Enzymology
https://www.readbyqxmd.com/read/29305015/alternative-splicing-in-genetic-diseases-improved-diagnosis-and-novel-treatment-options
#11
Atze J Bergsma, Erik van der Wal, Mike Broeders, Ans T van der Ploeg, W W M Pim Pijnappel
Alternative splicing is an important mechanism to regulate gene expression and to expand the repertoire of gene products in order to accommodate an increase in complexity of multicellular organisms. It needs to be precisely regulated, which is achieved via RNA structure, splicing factors, transcriptional regulation, and chromatin. Changes in any of these factors can lead to disease. These may include the core spliceosome, splicing enhancer/repressor sequences and their interacting proteins, the speed of transcription by RNA polymerase II, and histone modifications...
2018: International Review of Cell and Molecular Biology
https://www.readbyqxmd.com/read/29301363/current-advances-in-aptamers-for-cancer-diagnosis-and-therapy
#12
REVIEW
Shin-Ichiro Hori, Alberto Herrera, John J Rossi, Jiehua Zhou
Nucleic acid aptamers are single-stranded oligonucleotides that interact with target molecules with high affinity and specificity in unique three-dimensional structures. Aptamers are generally isolated by a simple selection process called systematic evolution of ligands by exponential enrichment (SELEX) and then can be chemically synthesized and modified. Because of their high affinity and specificity, aptamers are promising agents for biomarker discovery, as well as cancer diagnosis and therapy. In this review, we present recent progress and challenges in aptamer and SELEX technology and highlight some representative applications of aptamers in cancer therapy...
January 3, 2018: Cancers
https://www.readbyqxmd.com/read/29299639/b7-h3-in-tumors-friend-or-foe-for-tumor-immunity
#13
REVIEW
Gen Li, Yanchun Quan, Fengyuan Che, Lijuan Wang
B7-H3 is a type I transmembrane co-stimulatory molecule of the B7 family. B7-H3 mRNA is widely distributed in most tissues; however, B7-H3 protein is not constitutively expressed. Few molecules have been shown to mediate the regulation of B7-H3 expression, and their regulatory mechanisms remain unexplored. Recently, TREM-like transcript 2 (TLT-2) has been identified as a potential receptor of B7-H3. However, TLT-2 may not be the only receptor of B7-H3, as B7-H3 has many contradictory roles. As a co-stimulatory molecule, B7-H3 increases the proliferation of both CD4+ and CD8+ T-cells and enhances cytotoxic T-cell activity...
January 3, 2018: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/29297294/combining-agent-based-models-and-virtual-screening-techniques-to-predict-the-best-citrus-derived-vaccine-adjuvants-against-human-papilloma-virus
#14
Marzio Pennisi, Giulia Russo, Silvia Ravalli, Francesco Pappalardo
BACKGROUND: Human papillomavirus infection is a global social burden that, every year, leads to thousands new diagnosis of cancer. The introduction of a protocol of immunization, with Gardasil and Cervarix vaccines, has radically changed the way this infection easily spreads among people. Even though vaccination is only preventive and not therapeutic, it is a strong tool capable to avoid the consequences that this pathogen could cause. Gardasil vaccine is not free from side effects and the duration of immunity is not always well determined...
December 28, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/29295693/novel-homeodomain-transcription-factor-nkx2-2-in-the-brain-tumor-development
#15
Mubeena Pm Mariyath, Mehdi Hayat Shahi, Shirin Farheen, Mohd Tayyab, Nabeela Khanam, Asif Ali
BACKGROUND: Complex central nervous system (CNS) is made of neuronal cells and glial cells. Cells of central nervous system are able to regenerate after injury and during repairing. Sonic hedgehog pathway initiated by Shh-N a glycoprotein plays vital role in CNS patterning growth, development and now tumorigenesis. Nkx2.2 homeodomain transcription factor is an effecter molecule, which is positively regulated by Shh during normal growth. Nkx2.2 is essential for V3 domain specification during neural tube patterning at embryonic stage...
January 1, 2018: Current Cancer Drug Targets
https://www.readbyqxmd.com/read/29291225/application-of-viromics-a-new-approach-to-the-understanding-of-viral-infections-in-humans
#16
REVIEW
Mageshbabu Ramamurthy, Sathish Sankar, Rajesh Kannangai, Balaji Nandagopal, Gopalan Sridharan
This review is focused at exploring the strengths of modern technology driven data compiled in the areas of virus gene sequencing, virus protein structures and their implication to viral diagnosis and therapy. The information for virome analysis (viromics) is generated by the study of viral genomes (entire nucleotide sequence) and viral genes (coding for protein). Presently, the study of viral infectious diseases in terms of etiopathogenesis and development of newer therapeutics is undergoing rapid changes...
December 2017: Virusdisease
https://www.readbyqxmd.com/read/29291166/two-color-based-nanoflares-for-multiplexed-micrornas-imaging-in-live-cells
#17
Jing Li, Jin Huang, Xiaohai Yang, Yanjing Yang, Ke Quan, Nuli Xie, Yanan Wu, Changbei Ma, Kemin Wang
MicroRNAs (miRNAs) have become an ideal biomarker candidate for early diagnosis of diseases. But various diseases involve changes in the expression of different miRNAs. Therefore, multiplexed assay of miRNAs in live cells can provide critical information for our better understanding of their roles in cells and further validating of their function in clinical diagnoses. Simultaneous detection of multiple biomarkers could effectively improve the accuracy of early cancer diagnosis. Here, we develop the two-color-based nanoflares for simultaneously detecting two distinct miRNA targets inside live cells...
2018: Nanotheranostics
https://www.readbyqxmd.com/read/29290774/ncrnas-and-cholangiocarcinoma
#18
REVIEW
Zheng Wangyang, Ji Daolin, Xu Yi, Li Zhenglong, Huang Lining, Cui Yunfu, Jiang Xingming
Cholangiocarcinoma (CCA) is the most common primary biliary malignancy with poor prognosis. Less understanding of its etiology and pathogenesis makes the diagnosis and therapy difficult. Recently, accumulating evidences have demonstrated that deregulated expression of non-coding RNAs (ncRNAs) is closely associated with the etiopathogenesis of CCA. NcRNAs which lack open reading frame are a heterogeneous class of transcribed RNA molecules, including microRNAs, long non-coding RNAs and circular RNAs. Several studies have shown ncRNAs dysregulation is a common central event occurring in CCA and has the potential of being therapy targets...
2018: Journal of Cancer
https://www.readbyqxmd.com/read/29288091/the-importance-of-accounting-for-sex-in-the-search-of-proteomic-signatures-of-mycotoxin-exposure
#19
L Soler, I P Oswald
Mycotoxins are natural food and feed contaminants that are toxic to human and animals. Proteomics is an adequate toolbox to investigate the mode of action and the effects of mycotoxins, as these toxicants often alter protein synthesis and degradation, as well as induce changes of important post-translational modifications. For instance, the contaminant deoxynivalenol induces a severe ribosomal stress that affects protein production, whereas the toxin Fumonisin B1 can alter the phosphorylation of a large number of proteins, and patulin is a potent proteotoxic molecule...
December 26, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29287020/state-of-the-art-diagnosis-and-treatment-of-melanoma-optimal-multidetector-computed-tomographic-practice-to-identify-metastatic-disease-and-review-of-innovative-therapeutic-agents
#20
Blake C Jones, Evan J Lipson, Brandon Childers, Elliot K Fishman, Pamela T Johnson
The incidence of melanoma has risen dramatically over the past several decades. Oncologists rely on the ability of radiologists to identify subtle radiographic changes representing metastatic and recurrent melanoma in uncommon locations on multidetector computed tomography (MDCT) as the front-line imaging surveillance tool. To accomplish this goal, MDCT acquisition and display must be optimized and radiologist interpretation and search patterns must be tailored to identify the unique and often subtle metastatic lesions of melanoma...
December 28, 2017: Journal of Computer Assisted Tomography
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